AlfalfaGEDB | Msa0322680

M. sativa cultivar zhongmu-4 / Msa0322680

KEGG: K12191
GO: GO:0000815 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0006810 GO:0006900 GO:0006996 GO:0007032 GO:0008104 GO:0008150 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0036452 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0051179 GO:0051234 GO:0061024 GO:0070676 GO:0071702 GO:0071705 GO:0071840 GO:0097708 GO:0098588 GO:0098796 GO:0098805
PFAM: PF03357.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0322680	XP_013463507.1	91.928	223	3	2	1	208	1	223	1.18e-138	397

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0322680	sp\|Q9SKI2\|VPS2A_ARATH	82.143	224	24	4	1	208	2	225	9.81e-110	316

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002560	Msa0322680	0.809453	1.878209e-50	-8.615850e-47
Msa0003630	Msa0322680	0.832587	8.682742e-56	-8.615850e-47
Msa0005270	Msa0322680	0.815656	8.268906e-52	-8.615850e-47
Msa0007990	Msa0322680	0.824163	9.367948e-54	-8.615850e-47
Msa0008220	Msa0322680	0.836907	7.107586e-57	-8.615850e-47
Msa0010770	Msa0322680	0.812079	5.077868e-51	-8.615850e-47
Msa0012710	Msa0322680	0.842000	3.376897e-58	-8.615850e-47
Msa0015140	Msa0322680	0.801661	8.105391e-49	-8.615850e-47
Msa0017320	Msa0322680	0.845090	5.043401e-59	-8.615850e-47
Msa0022550	Msa0322680	0.807997	3.845841e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0322680	MtrunA17_Chr2g0300321	91.928	223	3	2	1	208	1	223	1.08e-142	397
Msa0322680	MtrunA17_Chr8g0343561	75.446	224	39	2	1	208	1	224	2.77e-94	274
Msa0322680	MtrunA17_Chr1g0183621	41.232	211	113	5	4	206	7	214	3.68e-43	144
Msa0322680	MtrunA17_Chr2g0303251	37.850	214	124	3	4	208	3	216	3.15e-36	126
Msa0322680	MtrunA17_Chr7g0261511	42.105	209	114	4	4	206	3	210	6.23e-32	115
Msa0322680	MtrunA17_Chr4g0028231	29.530	149	100	3	4	148	7	154	4.84e-15	71.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0322680	AT2G06530.1	82.143	224	24	4	1	208	2	225	9.97e-111	316
Msa0322680	AT2G06530.2	81.373	204	22	4	21	208	1	204	2.11e-96	279
Msa0322680	AT1G03950.1	37.915	211	120	5	4	206	3	210	1.91e-41	140
Msa0322680	AT5G44560.4	40.230	174	101	1	4	174	3	176	2.92e-39	133
Msa0322680	AT5G44560.1	40.230	174	101	1	4	174	3	176	7.50e-39	133
Msa0322680	AT5G44560.2	41.007	139	82	0	36	174	18	156	5.82e-33	117
Msa0322680	AT5G44560.3	38.686	137	81	1	4	137	3	139	1.15e-26	100
Msa0322680	AT5G22950.1	28.251	223	139	8	4	206	7	228	7.43e-13	65.5

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Find 73 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCGTGACAAAGGCCAT+GGG	0.584059	2_4:+25968393	Msa0322680:CDS
AACAGACGAATTAGTGAACC+AGG	0.607314	2_4:+25968560	Msa0322680:CDS
AACGCCAAATAATCACTTCT+CGG	0.425655	2_4:+25964488	Msa0322680:five_prime_UTR
AACTTAATGATCTACGATCG+TGG	0.641387	2_4:-25964514	None:intergenic
AAGCTAAAATCACAGCTCCA+AGG	0.625189	2_4:+25968105	Msa0322680:CDS
AAGGCGTGACAAAGGCCATG+GGG	0.748829	2_4:+25968394	Msa0322680:CDS
AAGTGCCAAACAAGGCCAGA+TGG	0.593007	2_4:+25965422	Msa0322680:CDS
AATCAATTAGGGAAATTGAG+CGG	0.682677	2_4:+25965347	Msa0322680:CDS
AATGTTGGATAAATCAATTA+GGG	0.485724	2_4:+25965336	Msa0322680:CDS
ACAACTTGTCAATCATAGCA+TGG	0.653495	2_4:-25968926	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATGAAAACGAATTATTTCG+TGG	+	chr2_4:25964394-25964413	Msa0322680:five_prime_UTR	30.0%
GATCTATTCGATGAGAAAAA+CGG	-	chr2_4:25964540-25964559	None:intergenic	30.0%
TAAATCTTCCAGTTAAGACT+TGG	-	chr2_4:25965118-25965137	None:intergenic	30.0%
AATCAATTAGGGAAATTGAG+CGG	+	chr2_4:25965347-25965366	Msa0322680:CDS	30.0%
ACAAGAGAAGAAATTGATTG+CGG	+	chr2_4:25965389-25965408	Msa0322680:CDS	30.0%
GCTACAACAAATAACTTCAT+AGG	-	chr2_4:25965479-25965498	None:intergenic	30.0%
ATTACCAGAATAATAGTGGT+GGG	-	chr2_4:25965563-25965582	None:intergenic	30.0%
TATTACCAGAATAATAGTGG+TGG	-	chr2_4:25965564-25965583	None:intergenic	30.0%
ACAACCTGATGTAAAACTAT+GGG	-	chr2_4:25966509-25966528	None:intergenic	30.0%
AACAACCTGATGTAAAACTA+TGG	-	chr2_4:25966510-25966529	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	25964336	25969180	25964336	ID=Msa0322680;Name=Msa0322680
chr2_4	mRNA	25964336	25969180	25964336	ID=Msa0322680-mRNA-1;Parent=Msa0322680;Name=Msa0322680-mRNA-1;_AED=0.05;_eAED=0.05;_QI=248\|0.6\|0.5\|1\|0.4\|0.5\|6\|319\|208
chr2_4	exon	25964336	25964620	25964336	ID=Msa0322680-mRNA-1:exon:9065;Parent=Msa0322680-mRNA-1
chr2_4	exon	25965314	25965378	25965314	ID=Msa0322680-mRNA-1:exon:9066;Parent=Msa0322680-mRNA-1
chr2_4	exon	25965387	25965443	25965387	ID=Msa0322680-mRNA-1:exon:9067;Parent=Msa0322680-mRNA-1
chr2_4	exon	25968042	25968146	25968042	ID=Msa0322680-mRNA-1:exon:9068;Parent=Msa0322680-mRNA-1
chr2_4	exon	25968357	25968581	25968357	ID=Msa0322680-mRNA-1:exon:9069;Parent=Msa0322680-mRNA-1
chr2_4	exon	25968724	25969180	25968724	ID=Msa0322680-mRNA-1:exon:9070;Parent=Msa0322680-mRNA-1
chr2_4	five_prime_UTR	25964336	25964583	25964336	ID=Msa0322680-mRNA-1:five_prime_utr;Parent=Msa0322680-mRNA-1
chr2_4	CDS	25964584	25964620	25964584	ID=Msa0322680-mRNA-1:cds;Parent=Msa0322680-mRNA-1
chr2_4	CDS	25965314	25965378	25965314	ID=Msa0322680-mRNA-1:cds;Parent=Msa0322680-mRNA-1
chr2_4	CDS	25965387	25965443	25965387	ID=Msa0322680-mRNA-1:cds;Parent=Msa0322680-mRNA-1
chr2_4	CDS	25968042	25968146	25968042	ID=Msa0322680-mRNA-1:cds;Parent=Msa0322680-mRNA-1
chr2_4	CDS	25968357	25968581	25968357	ID=Msa0322680-mRNA-1:cds;Parent=Msa0322680-mRNA-1
chr2_4	CDS	25968724	25968861	25968724	ID=Msa0322680-mRNA-1:cds;Parent=Msa0322680-mRNA-1
chr2_4	three_prime_UTR	25968862	25969180	25968862	ID=Msa0322680-mRNA-1:three_prime_utr;Parent=Msa0322680-mRNA-1

Gene Sequence

>Msa0322680

ATGAGTTTCATCTTCGGAAAGAGAAAAACACCCGCAGAGCTTCTGCGGGAAAACAAGAGAATGTTGGATAAATCAATTAGGGAAATTGAGCGGAGAGGCAAGACACAAGAGAAGAAATTGATTGCGGAGATAAAGAAAAGTGCCAAACAAGGCCAGATGGGAGCTGTTAGAGTCATGGCAAAAGATCTTGTTAGAACAAGGCATCAGATTGAAAAGTTTTACAAGCTAAAATCACAGCTCCAAGGTGTATCACTCAGAATTCAGACATTGAAATCAACACAAGCTATGGGTGAGGCGATGAAAGGCGTGACAAAGGCCATGGGGCAAATGAATAGGCAGATGAATTTGCCAAATTTGCAGAAAATTATGCAAGATTTCGAGAGACAGAATGAAAAGATGGAATTGACTATGGAAGTGATGGGAGATGCCATAGACGATGCTTTGGAAGGGGATGAAGAAGAAGAGGAAACAGACGAATTAGTGAACCAGCTTGTAAATGCACCATCATCAGCCGTTGCTGCCCCTGCTGCAAAGACGAAGGTACCTCAAGCTGAAACTGCTGCGACCGATGATAGTGGTATAGACAGTGATTTACAGGCAAGATTAGACAACTTAAGAAAAATGTAG

Protein sequence

>Msa0322680

MSFIFGKRKTPAELLRENKRMLDKSIREIERRGKTQEKKLIAEIKKSAKQGQMGAVRVMAKDLVRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPNLQKIMQDFERQNEKMELTMEVMGDAIDDALEGDEEEEETDELVNQLVNAPSSAVAAPAAKTKVPQAETAATDDSGIDSDLQARLDNLRKM*