AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0597000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0597000 MtrunA17_Chr4g0019091 100.000 86 0 0 1 86 313 398 3.67e-57 181
Msa0597000 MtrunA17_Chr1g0211041 90.698 86 8 0 1 86 315 400 6.94e-52 168
Msa0597000 MtrunA17_Chr4g0019071 84.884 86 13 0 1 86 190 275 6.71e-51 162
Msa0597000 MtrunA17_Chr4g0064541 69.767 86 26 0 1 86 312 397 2.87e-37 129
Msa0597000 MtrunA17_Chr3g0121411 51.190 84 30 1 2 85 366 438 2.11e-20 83.6
Msa0597000 MtrunA17_Chr6g0453731 48.780 82 31 1 1 82 365 435 3.07e-20 83.2
Msa0597000 MtrunA17_Chr3g0121431 51.190 84 30 1 2 85 421 493 1.61e-19 80.9
Msa0597000 MtrunA17_Chr7g0236041 48.810 84 32 1 2 85 365 437 1.84e-19 80.9
Msa0597000 MtrunA17_Chr7g0266131 48.235 85 33 1 1 85 298 371 2.37e-19 80.5
Msa0597000 MtrunA17_Chr1g0178831 46.512 86 33 2 1 85 290 363 1.58e-17 75.5
Msa0597000 MtrunA17_Chr3g0098721 49.412 85 32 1 1 85 286 359 6.01e-17 73.6
Msa0597000 MtrunA17_Chr5g0443881 49.412 85 32 2 1 85 292 365 1.78e-16 72.4
Msa0597000 MtrunA17_Chr7g0266151 47.727 88 33 2 1 86 290 366 1.19e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0597000 AT2G16430.1 91.860 86 7 0 1 86 197 282 2.86e-54 171
Msa0597000 AT2G16430.2 91.860 86 7 0 1 86 317 402 3.26e-53 172
Msa0597000 AT3G46120.1 75.294 85 21 0 1 85 237 321 8.60e-46 150
Msa0597000 AT2G27190.1 79.070 86 18 0 1 86 318 403 6.00e-45 150
Msa0597000 AT2G18130.1 72.941 85 23 0 1 85 294 378 1.60e-44 148
Msa0597000 AT1G56360.1 71.765 85 24 0 1 85 313 397 4.17e-42 142
Msa0597000 AT4G36350.1 72.941 85 23 0 1 85 313 397 7.66e-42 142
Msa0597000 AT5G34850.1 68.235 85 27 0 1 85 311 395 8.14e-35 123
Msa0597000 AT4G13700.1 52.439 82 28 1 1 82 359 429 3.73e-21 85.9
Msa0597000 AT3G52820.1 49.412 85 32 1 1 85 291 364 2.07e-19 80.9
Msa0597000 AT3G20500.1 49.412 85 32 1 1 85 290 363 4.46e-19 80.1
Msa0597000 AT3G52810.1 48.235 85 33 1 1 85 295 368 6.37e-19 79.7
Msa0597000 AT3G52780.1 50.000 88 31 2 1 86 290 366 2.32e-18 78.2
Msa0597000 AT3G07130.1 47.619 84 33 1 2 85 359 431 1.90e-17 75.5
Msa0597000 AT2G32770.2 46.053 76 30 1 4 79 300 364 6.30e-15 68.2
Msa0597000 AT2G32770.1 46.053 76 30 1 4 79 352 416 6.98e-15 68.2
Msa0597000 AT2G32770.3 46.053 76 30 1 4 79 381 445 7.29e-15 68.2
Msa0597000 AT3G07130.2 34.188 117 33 2 2 85 359 464 1.64e-11 58.5

Find 29 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTGTCCTGTTAACTTT+TGG 0.327184 4_2:+28772423 None:intergenic
GTTGCATATAATGTTGTAAA+TGG 0.349337 4_2:-28772019 Msa0597000:CDS
ACTTGGTTGCTAAGCCTTCA+AGG 0.359090 4_2:+28771933 None:intergenic
AGGAGCTACCAAAAGTTAAC+AGG 0.410375 4_2:-28772431 Msa0597000:five_prime_UTR
AGCACTTACAGAACGTTCAT+AGG 0.420944 4_2:+28772257 None:intergenic
TCTCCAATGGTTATGTATAC+AGG 0.430699 4_2:+28771965 None:intergenic
GGAGATGGAGGGAACCTTGA+AGG 0.431924 4_2:-28771947 Msa0597000:CDS
AATATACACCACAATACAAA+TGG 0.460805 4_2:-28772461 Msa0597000:five_prime_UTR
ACAATACAAATGGCTTGAAC+AGG 0.479670 4_2:-28772451 Msa0597000:five_prime_UTR
TACAACATTATATGCAACAT+TGG 0.493313 4_2:+28772024 None:intergenic
TACAATTATCATTACATGGA+AGG 0.507625 4_2:-28772357 Msa0597000:CDS
TTAACAGGACAGAAACTCCT+TGG 0.513520 4_2:-28772416 Msa0597000:five_prime_UTR
GCCATGGTTTGTTAAGTACA+AGG 0.515158 4_2:-28772310 Msa0597000:CDS
AAGGTTGATGTTGTGTATGC+TGG 0.518804 4_2:-28772291 Msa0597000:CDS
ACAATTATCATTACATGGAA+GGG 0.525105 4_2:-28772356 Msa0597000:CDS
TGTTCAAGCCATTTGTATTG+TGG 0.526598 4_2:+28772453 None:intergenic
TACATAACCATTGGAGATGG+AGG 0.550025 4_2:-28771959 Msa0597000:CDS
GCTCCTGTATACATAACCAT+TGG 0.553714 4_2:-28771968 Msa0597000:CDS
AAGGTTCCCTCCATCTCCAA+TGG 0.563747 4_2:+28771952 None:intergenic
CAATTATCATTACATGGAAG+GGG 0.564593 4_2:-28772355 Msa0597000:CDS
TTGTTCTCATGCATTCACCT+TGG 0.582761 4_2:-28772389 Msa0597000:exon
TAGCTACAATTATCATTACA+TGG 0.588515 4_2:-28772361 Msa0597000:CDS
GTATACATAACCATTGGAGA+TGG 0.590351 4_2:-28771962 Msa0597000:CDS
ACCTTGTACTTAACAAACCA+TGG 0.616531 4_2:+28772309 None:intergenic
TGAGAGTAATGTATGAGCCA+TGG 0.618209 4_2:-28772326 Msa0597000:CDS
AACGTTCATAGGCATGCACA+TGG 0.651957 4_2:+28772268 None:intergenic
ACATAACCATTGGAGATGGA+GGG 0.652593 4_2:-28771958 Msa0597000:CDS
TGCATGAGAACAATCAACCA+AGG 0.682000 4_2:+28772399 None:intergenic
TAATTGTAGCTATTATACCA+AGG 0.727202 4_2:+28772372 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAATTTA+TGG + chr4_2:28772254-28772273 None:intergenic 0.0%
!! AAAAAAAAAAAAAAAATTTA+TGG + chr4_2:28772254-28772273 None:intergenic 0.0%
!! AATTTGATTATAACGAAAAT+TGG + chr4_2:28772176-28772195 None:intergenic 15.0%
!!! AATTGATTTATTATTTCGTT+TGG - chr4_2:28772307-28772326 Msa0597000:CDS 15.0%
!! AATTTGATTATAACGAAAAT+TGG + chr4_2:28772176-28772195 None:intergenic 15.0%
!!! AATTGATTTATTATTTCGTT+TGG - chr4_2:28772307-28772326 Msa0597000:CDS 15.0%
!!! TTCAGATTTTTTCCATTTAA+TGG - chr4_2:28772195-28772214 Msa0597000:intron 20.0%
!!! TTCAGATTTTTTCCATTTAA+TGG - chr4_2:28772195-28772214 Msa0597000:intron 20.0%
! AATATACACCACAATACAAA+TGG - chr4_2:28771940-28771959 Msa0597000:CDS 25.0%
! TAATTGTAGCTATTATACCA+AGG + chr4_2:28772032-28772051 None:intergenic 25.0%
! TAGCTACAATTATCATTACA+TGG - chr4_2:28772040-28772059 Msa0597000:intron 25.0%
! TACAATTATCATTACATGGA+AGG - chr4_2:28772044-28772063 Msa0597000:intron 25.0%
! ACAATTATCATTACATGGAA+GGG - chr4_2:28772045-28772064 Msa0597000:intron 25.0%
! TACAACATTATATGCAACAT+TGG + chr4_2:28772380-28772399 None:intergenic 25.0%
! GTTGCATATAATGTTGTAAA+TGG - chr4_2:28772382-28772401 Msa0597000:CDS 25.0%
!! TGTATTGTGGTGTATATTTT+CGG + chr4_2:28771938-28771957 None:intergenic 25.0%
! AATATACACCACAATACAAA+TGG - chr4_2:28771940-28771959 Msa0597000:CDS 25.0%
! TAATTGTAGCTATTATACCA+AGG + chr4_2:28772032-28772051 None:intergenic 25.0%
! TAGCTACAATTATCATTACA+TGG - chr4_2:28772040-28772059 Msa0597000:intron 25.0%
! TACAATTATCATTACATGGA+AGG - chr4_2:28772044-28772063 Msa0597000:intron 25.0%
! ACAATTATCATTACATGGAA+GGG - chr4_2:28772045-28772064 Msa0597000:intron 25.0%
! TACAACATTATATGCAACAT+TGG + chr4_2:28772380-28772399 None:intergenic 25.0%
! GTTGCATATAATGTTGTAAA+TGG - chr4_2:28772382-28772401 Msa0597000:CDS 25.0%
CAATTATCATTACATGGAAG+GGG - chr4_2:28772046-28772065 Msa0597000:intron 30.0%
! TTTTGCAAGTTGCCATTAAA+TGG + chr4_2:28772210-28772229 None:intergenic 30.0%
ACAAACAAATCAGTTGTTAC+AGG + chr4_2:28772342-28772361 None:intergenic 30.0%
CAATTATCATTACATGGAAG+GGG - chr4_2:28772046-28772065 Msa0597000:intron 30.0%
! TTTTGCAAGTTGCCATTAAA+TGG + chr4_2:28772210-28772229 None:intergenic 30.0%
ACAAACAAATCAGTTGTTAC+AGG + chr4_2:28772342-28772361 None:intergenic 30.0%
TGTTCAAGCCATTTGTATTG+TGG + chr4_2:28771951-28771970 None:intergenic 35.0%
ACAATACAAATGGCTTGAAC+AGG - chr4_2:28771950-28771969 Msa0597000:CDS 35.0%
! GTTTCTGTCCTGTTAACTTT+TGG + chr4_2:28771981-28772000 None:intergenic 35.0%
ACCTTGTACTTAACAAACCA+TGG + chr4_2:28772095-28772114 None:intergenic 35.0%
!! ACAACTGATTTGTTTGTGTC+AGG - chr4_2:28772345-28772364 Msa0597000:CDS 35.0%
! GGAGCTGATTGATCTTTTAT+GGG + chr4_2:28772418-28772437 None:intergenic 35.0%
! AGGAGCTGATTGATCTTTTA+TGG + chr4_2:28772419-28772438 None:intergenic 35.0%
TCTCCAATGGTTATGTATAC+AGG + chr4_2:28772439-28772458 None:intergenic 35.0%
GTATACATAACCATTGGAGA+TGG - chr4_2:28772439-28772458 Msa0597000:five_prime_UTR 35.0%
TGTTCAAGCCATTTGTATTG+TGG + chr4_2:28771951-28771970 None:intergenic 35.0%
ACAATACAAATGGCTTGAAC+AGG - chr4_2:28771950-28771969 Msa0597000:CDS 35.0%
! GTTTCTGTCCTGTTAACTTT+TGG + chr4_2:28771981-28772000 None:intergenic 35.0%
ACCTTGTACTTAACAAACCA+TGG + chr4_2:28772095-28772114 None:intergenic 35.0%
!! ACAACTGATTTGTTTGTGTC+AGG - chr4_2:28772345-28772364 Msa0597000:CDS 35.0%
! GGAGCTGATTGATCTTTTAT+GGG + chr4_2:28772418-28772437 None:intergenic 35.0%
! AGGAGCTGATTGATCTTTTA+TGG + chr4_2:28772419-28772438 None:intergenic 35.0%
TCTCCAATGGTTATGTATAC+AGG + chr4_2:28772439-28772458 None:intergenic 35.0%
GTATACATAACCATTGGAGA+TGG - chr4_2:28772439-28772458 Msa0597000:five_prime_UTR 35.0%
AGGAGCTACCAAAAGTTAAC+AGG - chr4_2:28771970-28771989 Msa0597000:CDS 40.0%
TTAACAGGACAGAAACTCCT+TGG - chr4_2:28771985-28772004 Msa0597000:CDS 40.0%
TGCATGAGAACAATCAACCA+AGG + chr4_2:28772005-28772024 None:intergenic 40.0%
TTGTTCTCATGCATTCACCT+TGG - chr4_2:28772012-28772031 Msa0597000:CDS 40.0%
TGAGAGTAATGTATGAGCCA+TGG - chr4_2:28772075-28772094 Msa0597000:intron 40.0%
!! GCCATGGTTTGTTAAGTACA+AGG - chr4_2:28772091-28772110 Msa0597000:intron 40.0%
!! AAGGTTGATGTTGTGTATGC+TGG - chr4_2:28772110-28772129 Msa0597000:intron 40.0%
AGCACTTACAGAACGTTCAT+AGG + chr4_2:28772147-28772166 None:intergenic 40.0%
GCTCCTGTATACATAACCAT+TGG - chr4_2:28772433-28772452 Msa0597000:five_prime_UTR 40.0%
TACATAACCATTGGAGATGG+AGG - chr4_2:28772442-28772461 Msa0597000:five_prime_UTR 40.0%
ACATAACCATTGGAGATGGA+GGG - chr4_2:28772443-28772462 Msa0597000:five_prime_UTR 40.0%
AGGAGCTACCAAAAGTTAAC+AGG - chr4_2:28771970-28771989 Msa0597000:CDS 40.0%
TTAACAGGACAGAAACTCCT+TGG - chr4_2:28771985-28772004 Msa0597000:CDS 40.0%
TGCATGAGAACAATCAACCA+AGG + chr4_2:28772005-28772024 None:intergenic 40.0%
TTGTTCTCATGCATTCACCT+TGG - chr4_2:28772012-28772031 Msa0597000:CDS 40.0%
TGAGAGTAATGTATGAGCCA+TGG - chr4_2:28772075-28772094 Msa0597000:intron 40.0%
!! GCCATGGTTTGTTAAGTACA+AGG - chr4_2:28772091-28772110 Msa0597000:intron 40.0%
!! AAGGTTGATGTTGTGTATGC+TGG - chr4_2:28772110-28772129 Msa0597000:intron 40.0%
AGCACTTACAGAACGTTCAT+AGG + chr4_2:28772147-28772166 None:intergenic 40.0%
GCTCCTGTATACATAACCAT+TGG - chr4_2:28772433-28772452 Msa0597000:five_prime_UTR 40.0%
TACATAACCATTGGAGATGG+AGG - chr4_2:28772442-28772461 Msa0597000:five_prime_UTR 40.0%
ACATAACCATTGGAGATGGA+GGG - chr4_2:28772443-28772462 Msa0597000:five_prime_UTR 40.0%
AACGTTCATAGGCATGCACA+TGG + chr4_2:28772136-28772155 None:intergenic 45.0%
AACGTTCATAGGCATGCACA+TGG + chr4_2:28772136-28772155 None:intergenic 45.0%
AAGGTTCCCTCCATCTCCAA+TGG + chr4_2:28772452-28772471 None:intergenic 50.0%
AAGGTTCCCTCCATCTCCAA+TGG + chr4_2:28772452-28772471 None:intergenic 50.0%
GGAGATGGAGGGAACCTTGA+AGG - chr4_2:28772454-28772473 Msa0597000:five_prime_UTR 55.0%
GGAGATGGAGGGAACCTTGA+AGG - chr4_2:28772454-28772473 Msa0597000:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 28771937 28772486 28771937 ID=Msa0597000;Name=Msa0597000
chr4_2 mRNA 28771937 28772486 28771937 ID=Msa0597000-mRNA-1;Parent=Msa0597000;Name=Msa0597000-mRNA-1;_AED=0.11;_eAED=0.11;_QI=83|1|0|1|1|1|2|0|86
chr4_2 exon 28771937 28772056 28771937 ID=Msa0597000-mRNA-1:exon:7353;Parent=Msa0597000-mRNA-1
chr4_2 exon 28772266 28772486 28772266 ID=Msa0597000-mRNA-1:exon:7352;Parent=Msa0597000-mRNA-1
chr4_2 five_prime_UTR 28772404 28772486 28772404 ID=Msa0597000-mRNA-1:five_prime_utr;Parent=Msa0597000-mRNA-1
chr4_2 CDS 28772266 28772403 28772266 ID=Msa0597000-mRNA-1:cds;Parent=Msa0597000-mRNA-1
chr4_2 CDS 28771937 28772056 28771937 ID=Msa0597000-mRNA-1:cds;Parent=Msa0597000-mRNA-1
Gene Sequence

>Msa0597000

ATGCATTCACCTTGGTATAATAGCTACAATTATCATTACATGGAAGGGGAATCAATGAGAGTAATGTATGAGCCATGGTTTGTTAAGTACAAGGTTGATGTTGTGTATGCTGGCCATGTGCATGCCTATGAACGTTCTGAACGTGTGTCCAATGTTGCATATAATGTTGTAAATGGTATTTGCACTCCCATAAAAGATCAATCAGCTCCTGTATACATAACCATTGGAGATGGAGGGAACCTTGAAGGCTTAGCAACC

Protein sequence

>Msa0597000

MHSPWYNSYNYHYMEGESMRVMYEPWFVKYKVDVVYAGHVHAYERSERVSNVAYNVVNGICTPIKDQSAPVYITIGDGGNLEGLAT