Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0093400 | XP_003597750.1 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 635 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0093400 | G7II20 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 635 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001590 | Msa0093400 | 0.815817 | 7.611386e-52 | -8.615850e-47 |
| Msa0017510 | Msa0093400 | 0.829258 | 5.693033e-55 | -8.615850e-47 |
| Msa0025330 | Msa0093400 | 0.804565 | 2.032165e-49 | -8.615850e-47 |
| Msa0034270 | Msa0093400 | 0.804637 | 1.963321e-49 | -8.615850e-47 |
| Msa0046100 | Msa0093400 | 0.813942 | 1.982829e-51 | -8.615850e-47 |
| Msa0093400 | Msa0094850 | 0.819468 | 1.143370e-52 | -8.615850e-47 |
| Msa0093400 | Msa0108160 | 0.831975 | 1.230397e-55 | -8.615850e-47 |
| Msa0093400 | Msa0110970 | 0.813019 | 3.163725e-51 | -8.615850e-47 |
| Msa0093400 | Msa0152430 | 0.811974 | 5.353148e-51 | -8.615850e-47 |
| Msa0093400 | Msa0157290 | 0.829361 | 5.374109e-55 | -8.615850e-47 |
| Msa0093400 | Msa0160130 | 0.801799 | 7.593075e-49 | -8.615850e-47 |
| Msa0093400 | Msa0165430 | 0.805461 | 1.320235e-49 | -8.615850e-47 |
| Msa0093400 | Msa0212990 | 0.810211 | 1.290141e-50 | -8.615850e-47 |
| Msa0093400 | Msa0223690 | 0.805546 | 1.267035e-49 | -8.615850e-47 |
| Msa0093400 | Msa0225320 | 0.956041 | 7.484663e-114 | -8.615850e-47 |
| Msa0093400 | Msa0251050 | 0.821969 | 3.042406e-53 | -8.615850e-47 |
| Msa0093400 | Msa0283450 | 0.804946 | 1.691949e-49 | -8.615850e-47 |
| Msa0093400 | Msa0297740 | 0.804692 | 1.911856e-49 | -8.615850e-47 |
| Msa0093400 | Msa0306300 | 0.834483 | 2.920137e-56 | -8.615850e-47 |
| Msa0093400 | Msa0327750 | 0.806491 | 8.017834e-50 | -8.615850e-47 |
| Msa0093400 | Msa0330340 | 0.803083 | 4.129457e-49 | -8.615850e-47 |
| Msa0093400 | Msa0332000 | 0.804478 | 2.118926e-49 | -8.615850e-47 |
| Msa0093400 | Msa0334220 | 0.826981 | 2.012963e-54 | -8.615850e-47 |
| Msa0093400 | Msa0335040 | 0.803773 | 2.970780e-49 | -8.615850e-47 |
| Msa0093400 | Msa0341910 | 0.988191 | 4.619859e-173 | -8.615850e-47 |
| Msa0093400 | Msa0369850 | 0.802909 | 4.485602e-49 | -8.615850e-47 |
| Msa0093400 | Msa0388960 | 0.800782 | 1.226474e-48 | -8.615850e-47 |
| Msa0093400 | Msa0389340 | 0.804638 | 1.962434e-49 | -8.615850e-47 |
| Msa0093400 | Msa0410650 | 0.801534 | 8.607143e-49 | -8.615850e-47 |
| Msa0093400 | Msa0417270 | 0.807254 | 5.529697e-50 | -8.615850e-47 |
| Msa0093400 | Msa0417880 | 0.823516 | 1.328076e-53 | -8.615850e-47 |
| Msa0093400 | Msa0452960 | 0.819250 | 1.281854e-52 | -8.615850e-47 |
| Msa0093400 | Msa0461730 | 0.810772 | 9.761465e-51 | -8.615850e-47 |
| Msa0093400 | Msa0467430 | 0.803490 | 3.400131e-49 | -8.615850e-47 |
| Msa0093400 | Msa0469160 | 0.818926 | 1.518733e-52 | -8.615850e-47 |
| Msa0093400 | Msa0488560 | 0.800253 | 1.571581e-48 | -8.615850e-47 |
| Msa0093400 | Msa0495320 | 0.826001 | 3.447107e-54 | -8.615850e-47 |
| Msa0093400 | Msa0507680 | 0.815320 | 9.822763e-52 | -8.615850e-47 |
| Msa0093400 | Msa0509610 | 0.817202 | 3.725757e-52 | -8.615850e-47 |
| Msa0093400 | Msa0513160 | 0.808020 | 3.802911e-50 | -8.615850e-47 |
| Msa0093400 | Msa0545210 | 0.809992 | 1.438323e-50 | -8.615850e-47 |
| Msa0093400 | Msa0546680 | 0.812530 | 4.048627e-51 | -8.615850e-47 |
| Msa0093400 | Msa0567020 | 0.801488 | 8.793303e-49 | -8.615850e-47 |
| Msa0093400 | Msa0626450 | 0.820379 | 7.077004e-53 | -8.615850e-47 |
| Msa0093400 | Msa0626490 | 0.822185 | 2.712047e-53 | -8.615850e-47 |
| Msa0093400 | Msa0633700 | 0.814285 | 1.665261e-51 | -8.615850e-47 |
| Msa0093400 | Msa0657970 | 0.801850 | 7.411882e-49 | -8.615850e-47 |
| Msa0093400 | Msa0658570 | 0.804900 | 1.730013e-49 | -8.615850e-47 |
| Msa0093400 | Msa0664600 | 0.824918 | 6.221435e-54 | -8.615850e-47 |
| Msa0093400 | Msa0693380 | 0.805935 | 1.049864e-49 | -8.615850e-47 |
| Msa0093400 | Msa0703490 | 0.800760 | 1.238877e-48 | -8.615850e-47 |
| Msa0093400 | Msa0705870 | 0.807002 | 6.251438e-50 | -8.615850e-47 |
| Msa0093400 | Msa0712590 | 0.814666 | 1.371476e-51 | -8.615850e-47 |
| Msa0093400 | Msa0713000 | 0.800028 | 1.746050e-48 | -8.615850e-47 |
| Msa0093400 | Msa0759120 | 0.809398 | 1.929795e-50 | -8.615850e-47 |
| Msa0093400 | Msa0767300 | 0.802676 | 5.011048e-49 | -8.615850e-47 |
| Msa0093400 | Msa0795390 | 0.816728 | 4.761558e-52 | -8.615850e-47 |
| Msa0093400 | Msa0844240 | 0.825851 | 3.741470e-54 | -8.615850e-47 |
| Msa0093400 | Msa0870530 | 0.814456 | 1.526579e-51 | -8.615850e-47 |
| Msa0093400 | Msa0875290 | 0.803778 | 2.963684e-49 | -8.615850e-47 |
| Msa0093400 | Msa0880140 | 0.828113 | 1.076484e-54 | -8.615850e-47 |
| Msa0093400 | Msa0903700 | 0.818058 | 2.390019e-52 | -8.615850e-47 |
| Msa0093400 | Msa0909120 | 0.830815 | 2.374534e-55 | -8.615850e-47 |
| Msa0093400 | Msa0973150 | 0.816358 | 5.761546e-52 | -8.615850e-47 |
| Msa0093400 | Msa0996680 | 0.813069 | 3.084716e-51 | -8.615850e-47 |
| Msa0093400 | Msa1007270 | 0.805630 | 1.216811e-49 | -8.615850e-47 |
| Msa0093400 | Msa1024740 | 0.814026 | 1.899720e-51 | -8.615850e-47 |
| Msa0093400 | Msa1031930 | 0.822097 | 2.842316e-53 | -8.615850e-47 |
| Msa0093400 | Msa1043920 | 0.823442 | 1.382222e-53 | -8.615850e-47 |
| Msa0093400 | Msa1082500 | 0.819073 | 1.406445e-52 | -8.615850e-47 |
| Msa0093400 | Msa1085200 | 0.804249 | 2.365265e-49 | -8.615850e-47 |
| Msa0093400 | Msa1085300 | 0.802069 | 6.683799e-49 | -8.615850e-47 |
| Msa0093400 | Msa1104000 | 0.819647 | 1.040795e-52 | -8.615850e-47 |
| Msa0093400 | Msa1108160 | 0.806269 | 8.928629e-50 | -8.615850e-47 |
| Msa0093400 | Msa1108790 | 0.818201 | 2.217575e-52 | -8.615850e-47 |
| Msa0093400 | Msa1151910 | 0.826432 | 2.722137e-54 | -8.615850e-47 |
| Msa0093400 | Msa1152510 | 0.807078 | 6.026080e-50 | -8.615850e-47 |
| Msa0093400 | Msa1179940 | 0.817603 | 3.027145e-52 | -8.615850e-47 |
| Msa0093400 | Msa1179970 | 0.818210 | 2.207597e-52 | -8.615850e-47 |
| Msa0093400 | Msa1182860 | 0.821142 | 4.725679e-53 | -8.615850e-47 |
| Msa0093400 | Msa1183120 | 0.829109 | 6.187964e-55 | -8.615850e-47 |
| Msa0093400 | Msa1205290 | 0.822910 | 1.839647e-53 | -8.615850e-47 |
| Msa0093400 | Msa1211150 | -0.825347 | 4.924952e-54 | -8.615850e-47 |
| Msa0093400 | Msa1229500 | 0.831473 | 1.636504e-55 | -8.615850e-47 |
| Msa0093400 | Msa1236100 | 0.807918 | 3.997542e-50 | -8.615850e-47 |
| Msa0093400 | Msa1242220 | 0.807996 | 3.847451e-50 | -8.615850e-47 |
| Msa0093400 | Msa1270610 | 0.834135 | 3.571347e-56 | -8.615850e-47 |
| Msa0093400 | Msa1271300 | 0.811163 | 8.032718e-51 | -8.615850e-47 |
| Msa0093400 | Msa1271990 | 0.844469 | 7.414736e-59 | -8.615850e-47 |
| Msa0093400 | Msa1272900 | 0.813253 | 2.811066e-51 | -8.615850e-47 |
| Msa0093400 | Msa1278840 | 0.802749 | 4.840085e-49 | -8.615850e-47 |
| Msa0093400 | Msa1283860 | 0.825520 | 4.483482e-54 | -8.615850e-47 |
| Msa0093400 | Msa1299300 | -0.800122 | 1.671513e-48 | -8.615850e-47 |
| Msa0093400 | Msa1308380 | 0.827945 | 1.181554e-54 | -8.615850e-47 |
| Msa0093400 | Msa1322890 | 0.826738 | 2.300783e-54 | -8.615850e-47 |
| Msa0093400 | Msa1327050 | 0.814730 | 1.327320e-51 | -8.615850e-47 |
| Msa0093400 | Msa1335770 | 0.804861 | 1.762666e-49 | -8.615850e-47 |
| Msa0093400 | Msa1355800 | 0.989534 | 1.552699e-178 | -8.615850e-47 |
| Msa0093400 | Msa1376890 | 0.801933 | 7.127291e-49 | -8.615850e-47 |
| Msa0093400 | Msa1409870 | 0.806921 | 6.503753e-50 | -8.615850e-47 |
| Msa0093400 | Msa1423880 | 0.805507 | 1.291076e-49 | -8.615850e-47 |
| Msa0093400 | Msa1431430 | 0.829325 | 5.484290e-55 | -8.615850e-47 |
| Msa0093400 | Msa1440960 | 0.817981 | 2.487443e-52 | -8.615850e-47 |
| Msa0093400 | Msa1445190 | 0.807835 | 4.163029e-50 | -8.615850e-47 |
| Msa0093400 | Msa1452120 | 0.808736 | 2.675203e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0093400 | MtrunA17_Chr2g0331671 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 635 |
| Msa0093400 | MtrunA17_Chr6g0486001 | 52.885 | 312 | 135 | 6 | 2 | 308 | 1 | 305 | 4.85e-103 | 303 |
| Msa0093400 | MtrunA17_Chr6g0483441 | 38.699 | 292 | 153 | 5 | 22 | 309 | 25 | 294 | 9.74e-62 | 197 |
| Msa0093400 | MtrunA17_Chr8g0392551 | 41.667 | 312 | 162 | 7 | 2 | 309 | 1 | 296 | 9.92e-61 | 195 |
| Msa0093400 | MtrunA17_Chr4g0053001 | 40.994 | 322 | 169 | 9 | 4 | 309 | 14 | 330 | 1.15e-59 | 194 |
| Msa0093400 | MtrunA17_Chr4g0038691 | 36.050 | 319 | 177 | 10 | 6 | 309 | 12 | 318 | 1.35e-49 | 167 |
| Msa0093400 | MtrunA17_Chr3g0091821 | 31.138 | 167 | 99 | 4 | 128 | 293 | 26 | 177 | 2.93e-18 | 81.3 |
| Msa0093400 | MtrunA17_Chr8g0379281 | 37.755 | 98 | 55 | 2 | 137 | 234 | 106 | 197 | 6.43e-16 | 75.1 |
| Msa0093400 | MtrunA17_Chr3g0090061 | 22.977 | 309 | 198 | 8 | 27 | 297 | 29 | 335 | 9.45e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0093400 | AT1G45688.1 | 43.614 | 321 | 147 | 7 | 2 | 289 | 1 | 320 | 1.69e-69 | 219 |
| Msa0093400 | AT5G42860.1 | 40.645 | 310 | 146 | 5 | 2 | 287 | 1 | 296 | 3.01e-65 | 208 |
| Msa0093400 | AT3G24605.1 | 40.129 | 309 | 160 | 7 | 5 | 306 | 6 | 296 | 3.63e-64 | 204 |
| Msa0093400 | AT3G24600.1 | 41.613 | 310 | 168 | 9 | 1 | 306 | 1 | 301 | 1.63e-56 | 185 |
| Msa0093400 | AT1G45688.2 | 45.382 | 249 | 119 | 8 | 2 | 235 | 1 | 247 | 2.67e-52 | 172 |
| Msa0093400 | AT4G35170.1 | 35.789 | 285 | 159 | 9 | 14 | 287 | 6 | 277 | 2.39e-42 | 148 |
| Msa0093400 | AT2G41990.1 | 35.117 | 299 | 170 | 8 | 2 | 293 | 1 | 282 | 1.97e-39 | 140 |
| Msa0093400 | AT3G08490.1 | 22.932 | 266 | 179 | 4 | 27 | 291 | 11 | 251 | 1.82e-13 | 69.7 |
Find 78 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAGTGCTTTGAAATTAAA+AGG | 0.150017 | 1_2:-76151068 | None:intergenic |
| GGTAATAAGATTCCCCTTTA+TGG | 0.167603 | 1_2:+76153036 | Msa0093400:CDS |
| GAATTCTTCTTGAATTTAAT+AGG | 0.271825 | 1_2:-76153222 | None:intergenic |
| TCTCCTTCACATCCTTCTTT+TGG | 0.272863 | 1_2:+76151621 | Msa0093400:CDS |
| TCCATAGGACTGTTTGATAT+TGG | 0.301627 | 1_2:-76151597 | None:intergenic |
| AGTAGAAAGAGGAATGATAA+AGG | 0.322695 | 1_2:+76151714 | Msa0093400:CDS |
| ACAATACGGTGACTCTTTCT+TGG | 0.322732 | 1_2:-76152997 | None:intergenic |
| ACATAGTATACAGGTCTCTT+TGG | 0.336582 | 1_2:-76151516 | None:intergenic |
| AATGGAAATAAATGAGATAA+TGG | 0.364937 | 1_2:-76151173 | None:intergenic |
| GGGTGCTAGCAGGCCTTTCA+AGG | 0.389621 | 1_2:+76151875 | Msa0093400:CDS |
| AGGTGATGTGGTGGGGAAAC+TGG | 0.391294 | 1_2:+76153137 | Msa0093400:CDS |
| TAAGGAAATCACTTGTCCTT+TGG | 0.400348 | 1_2:+76153176 | Msa0093400:CDS |
| TAACAAGAAACTCAAAGAAA+TGG | 0.408295 | 1_2:-76151191 | None:intergenic |
| GGTCTCTTTGGAGATCTTGA+TGG | 0.410924 | 1_2:-76151504 | None:intergenic |
| CCTTTGGTTATCAATTCTGC+TGG | 0.414114 | 1_2:+76153192 | Msa0093400:CDS |
| CCATCATGTGAATCTCTTGA+AGG | 0.414191 | 1_2:-76151546 | None:intergenic |
| TTTCAGCAGCCAGTGATGTT+TGG | 0.414699 | 1_2:+76150991 | Msa0093400:five_prime_UTR |
| CAGTACATAATCTATATGTT+TGG | 0.415741 | 1_2:+76152662 | Msa0093400:CDS |
| TCAGAAACTTCTCTTGCAAA+TGG | 0.415938 | 1_2:-76153360 | None:intergenic |
| TGATCATTTGGGGTGCTAGC+AGG | 0.416591 | 1_2:+76151865 | Msa0093400:CDS |
| AGTACATAATCTATATGTTT+GGG | 0.418371 | 1_2:+76152663 | Msa0093400:CDS |
| TCCGGTTTGTGAAACTGTTA+TGG | 0.424945 | 1_2:-76153070 | None:intergenic |
| GTTTGCACTTTGCAAGGAAA+TGG | 0.429304 | 1_2:-76151116 | None:intergenic |
| TGGTTGATCCAGCTCCATAA+AGG | 0.434697 | 1_2:-76153050 | None:intergenic |
| AAGATTCCCCTTTATGGAGC+TGG | 0.446069 | 1_2:+76153042 | Msa0093400:CDS |
| GGTTGATCCAGCTCCATAAA+GGG | 0.452029 | 1_2:-76153049 | None:intergenic |
| ACCATAACAGTTTCACAAAC+CGG | 0.452618 | 1_2:+76153069 | Msa0093400:CDS |
| AAGTGCTACTTCAACACTTC+CGG | 0.453891 | 1_2:-76153088 | None:intergenic |
| CCGTATTGTATCAGTGAACT+TGG | 0.454362 | 1_2:+76153011 | Msa0093400:CDS |
| TCCAATATCAAACAGTCCTA+TGG | 0.455908 | 1_2:+76151596 | Msa0093400:CDS |
| AGGCTTGGAACAAAACATAA+AGG | 0.468103 | 1_2:-76151023 | None:intergenic |
| GGAGATCTTGATGGTGAAGA+TGG | 0.477710 | 1_2:-76151495 | None:intergenic |
| GTAGAAAGAGGAATGATAAA+GGG | 0.480419 | 1_2:+76151715 | Msa0093400:CDS |
| GGTAAGGACAAGGCATCATA+AGG | 0.484890 | 1_2:+76153158 | Msa0093400:CDS |
| CTAGTGCTTTGAAATTAAAA+GGG | 0.488026 | 1_2:-76151067 | None:intergenic |
| GGACTTTGTACATAGTATAC+AGG | 0.503085 | 1_2:-76151525 | None:intergenic |
| CTCGAGTGACGGCCAAAAGA+AGG | 0.504004 | 1_2:-76151633 | None:intergenic |
| CCTTCAAGAGATTCACATGA+TGG | 0.519036 | 1_2:+76151546 | Msa0093400:CDS |
| GAAGGAAAGGTAGTAGAAAG+AGG | 0.523236 | 1_2:+76151703 | Msa0093400:CDS |
| ACAGTAACTTCTGCCTTGAA+AGG | 0.531838 | 1_2:-76151888 | None:intergenic |
| AAGTAAAGTAAGTGATTGAA+AGG | 0.534784 | 1_2:-76151043 | None:intergenic |
| TCAGATATCAGTATCGCAAC+CGG | 0.536332 | 1_2:+76152814 | Msa0093400:CDS |
| AAGTAAGTGATTGAAAGGCT+TGG | 0.546484 | 1_2:-76151038 | None:intergenic |
| CCAAGTTCACTGATACAATA+CGG | 0.554928 | 1_2:-76153011 | None:intergenic |
| CAAGGCAGAAGTTACTGTCA+AGG | 0.562919 | 1_2:+76151893 | Msa0093400:CDS |
| GATCGCGAGGTGATGTGGTG+GGG | 0.563802 | 1_2:+76153130 | Msa0093400:CDS |
| CGATCGCGAGGTGATGTGGT+GGG | 0.564068 | 1_2:+76153129 | Msa0093400:CDS |
| TGATCGGTGAAGTGTGGACA+TGG | 0.570257 | 1_2:-76152780 | None:intergenic |
| AGGTCATCTTCAGGAAGGAA+AGG | 0.572600 | 1_2:+76151690 | Msa0093400:CDS |
| CGAGAAACTCTTCTGCTAGT+CGG | 0.574468 | 1_2:+76151652 | Msa0093400:CDS |
| TAAGAATGATCTTACCTCAC+CGG | 0.575350 | 1_2:-76152833 | None:intergenic |
| ATGTGGTGGGGAAACTGGTA+AGG | 0.575803 | 1_2:+76153142 | Msa0093400:CDS |
| TGTGGTGTTATACTTGAAGA+AGG | 0.580795 | 1_2:+76151747 | Msa0093400:CDS |
| GAGTTTGTTTGCACTTTGCA+AGG | 0.586534 | 1_2:-76151122 | None:intergenic |
| TCTGAGTAAACAAGATTGAT+CGG | 0.590368 | 1_2:-76152796 | None:intergenic |
| CTTTAAGAACCAAACATCAC+TGG | 0.592949 | 1_2:-76151000 | None:intergenic |
| CAGAAGAGTTTCTCGAGTGA+CGG | 0.597006 | 1_2:-76151644 | None:intergenic |
| AATCCGATCGCGAGGTGATG+TGG | 0.597666 | 1_2:+76153125 | Msa0093400:CDS |
| ATTGTATCAGTGAACTTGGA+AGG | 0.600518 | 1_2:+76153015 | Msa0093400:CDS |
| GGATGTGAAGGAGACTCCAT+AGG | 0.604242 | 1_2:-76151612 | None:intergenic |
| CGGCCAAAAGAAGGATGTGA+AGG | 0.605614 | 1_2:-76151624 | None:intergenic |
| ACATAATCTATATGTTTGGG+AGG | 0.606954 | 1_2:+76152666 | Msa0093400:CDS |
| TGATATTGGAGTAGCTTGCA+TGG | 0.609840 | 1_2:-76151583 | None:intergenic |
| CAAGATTGATCGGTGAAGTG+TGG | 0.610457 | 1_2:-76152786 | None:intergenic |
| CCACCACATCACCTCGCGAT+CGG | 0.610908 | 1_2:-76153128 | None:intergenic |
| AGGTTCTTATCGTGAATTCG+AGG | 0.611273 | 1_2:+76151767 | Msa0093400:CDS |
| CCGATCGCGAGGTGATGTGG+TGG | 0.611667 | 1_2:+76153128 | Msa0093400:CDS |
| TGGGGAAACTGGTAAGGACA+AGG | 0.623482 | 1_2:+76153148 | Msa0093400:CDS |
| CCAGCAGAATTGATAACCAA+AGG | 0.632275 | 1_2:-76153192 | None:intergenic |
| AAGTTTGAAATCCGATCGCG+AGG | 0.634220 | 1_2:+76153117 | Msa0093400:CDS |
| GTTGATCCAGCTCCATAAAG+GGG | 0.651471 | 1_2:-76153048 | None:intergenic |
| TCAAGTATAACACCACACTC+AGG | 0.656313 | 1_2:-76151741 | None:intergenic |
| GATAAAGGGTGGCCTGAGTG+TGG | 0.658555 | 1_2:+76151729 | Msa0093400:CDS |
| CATAATCTATATGTTTGGGA+GGG | 0.659908 | 1_2:+76152667 | Msa0093400:CDS |
| TATCAGTATCGCAACCGGTG+AGG | 0.671558 | 1_2:+76152819 | Msa0093400:CDS |
| GAAAGAGGAATGATAAAGGG+TGG | 0.678134 | 1_2:+76151718 | Msa0093400:CDS |
| TCACTGGCTGCTGAAAGTGT+CGG | 0.729310 | 1_2:-76150984 | None:intergenic |
| TGGGAGGGTACAGACCACAC+AGG | 0.759316 | 1_2:+76152682 | Msa0093400:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACAATTATAAAATCAGTT+TGG | + | chr1_2:76152084-76152103 | Msa0093400:intron | 15.0% |
| !! | AATGGAAATAAATGAGATAA+TGG | - | chr1_2:76151176-76151195 | None:intergenic | 20.0% |
| !! | AGACTTAAGAATAACAAAAT+TGG | - | chr1_2:76151922-76151941 | None:intergenic | 20.0% |
| !! | ATTAAACACTTAATGCATAA+AGG | - | chr1_2:76152152-76152171 | None:intergenic | 20.0% |
| !!! | AATAGTTTAACTTTTCTTTG+TGG | + | chr1_2:76152203-76152222 | Msa0093400:intron | 20.0% |
| !!! | AACTGTATTTTTAACTTTCA+AGG | - | chr1_2:76152370-76152389 | None:intergenic | 20.0% |
| !! | TTGAAAGTTAAAAATACAGT+TGG | + | chr1_2:76152369-76152388 | Msa0093400:intron | 20.0% |
| !! | AGAATAATATAGAAACATAC+AGG | - | chr1_2:76152476-76152495 | None:intergenic | 20.0% |
| !!! | AATTTTGTTTCAATCACTTT+GGG | + | chr1_2:76152517-76152536 | Msa0093400:intron | 20.0% |
| !! | TTAATTAATAATGCAGAGTT+TGG | + | chr1_2:76152639-76152658 | Msa0093400:intron | 20.0% |
| !! | AGTACATAATCTATATGTTT+GGG | + | chr1_2:76152663-76152682 | Msa0093400:CDS | 20.0% |
| !!! | TAATTGTCAGAATTTTTGTT+GGG | - | chr1_2:76152711-76152730 | None:intergenic | 20.0% |
| !!! | TTAATTGTCAGAATTTTTGT+TGG | - | chr1_2:76152712-76152731 | None:intergenic | 20.0% |
| !!! | AATTCAAGTAACTTTTTTCT+TGG | + | chr1_2:76152912-76152931 | Msa0093400:intron | 20.0% |
| !! | GAATTCTTCTTGAATTTAAT+AGG | - | chr1_2:76153225-76153244 | None:intergenic | 20.0% |
| ! | AAGTAAAGTAAGTGATTGAA+AGG | - | chr1_2:76151046-76151065 | None:intergenic | 25.0% |
| !!! | CTAGTGCTTTGAAATTAAAA+GGG | - | chr1_2:76151070-76151089 | None:intergenic | 25.0% |
| ! | TAACAAGAAACTCAAAGAAA+TGG | - | chr1_2:76151194-76151213 | None:intergenic | 25.0% |
| !!! | TGTTTTCTAGTACTATTTAC+TGG | + | chr1_2:76151302-76151321 | Msa0093400:five_prime_UTR | 25.0% |
| !!! | TGTGTTTTGTTTGATCATTT+GGG | + | chr1_2:76151854-76151873 | Msa0093400:CDS | 25.0% |
| ! | TTAATATCAGCCAACTAAAA+TGG | - | chr1_2:76152325-76152344 | None:intergenic | 25.0% |
| ! | CAAAGTGATTGAAACAAAAT+TGG | - | chr1_2:76152518-76152537 | None:intergenic | 25.0% |
| !!! | CAATTTTGTTTCAATCACTT+TGG | + | chr1_2:76152516-76152535 | Msa0093400:intron | 25.0% |
| !!! | TTCTTTTTTGGTACAAAAGA+GGG | - | chr1_2:76152548-76152567 | None:intergenic | 25.0% |
| !!! | TTTCTTTTTTGGTACAAAAG+AGG | - | chr1_2:76152549-76152568 | None:intergenic | 25.0% |
| !! | CTTTTGTACCAAAAAAGAAA+TGG | + | chr1_2:76152549-76152568 | Msa0093400:intron | 25.0% |
| !! | CAATACTACTTAATTTTCCA+AGG | - | chr1_2:76152603-76152622 | None:intergenic | 25.0% |
| ! | CAGTACATAATCTATATGTT+TGG | + | chr1_2:76152662-76152681 | Msa0093400:CDS | 25.0% |
| !!! | TTTGAGTATTTTTGAGATTG+AGG | + | chr1_2:76153333-76153352 | Msa0093400:three_prime_UTR | 25.0% |
| !! | GCTAGTGCTTTGAAATTAAA+AGG | - | chr1_2:76151071-76151090 | None:intergenic | 30.0% |
| AGTAGAAAGAGGAATGATAA+AGG | + | chr1_2:76151714-76151733 | Msa0093400:CDS | 30.0% | |
| GTAGAAAGAGGAATGATAAA+GGG | + | chr1_2:76151715-76151734 | Msa0093400:CDS | 30.0% | |
| !!! | CTGTGTTTTGTTTGATCATT+TGG | + | chr1_2:76151853-76151872 | Msa0093400:CDS | 30.0% |
| !!! | GTGTTTTGTTTGATCATTTG+GGG | + | chr1_2:76151855-76151874 | Msa0093400:CDS | 30.0% |
| !! | TTATTTCTACAAGCTCTCTT+GGG | + | chr1_2:76152039-76152058 | Msa0093400:intron | 30.0% |
| AACTAAAATGGATGCATAGT+AGG | - | chr1_2:76152313-76152332 | None:intergenic | 30.0% | |
| !! | TACTATGCATCCATTTTAGT+TGG | + | chr1_2:76152312-76152331 | Msa0093400:intron | 30.0% |
| TGATATTAACACTGACAGAA+AGG | + | chr1_2:76152336-76152355 | Msa0093400:intron | 30.0% | |
| !!! | TGCTACTACCATTTCTTTTT+TGG | - | chr1_2:76152560-76152579 | None:intergenic | 30.0% |
| !! | TTGCTATTTATAACGTTCCT+TGG | + | chr1_2:76152583-76152602 | Msa0093400:intron | 30.0% |
| ACATAATCTATATGTTTGGG+AGG | + | chr1_2:76152666-76152685 | Msa0093400:CDS | 30.0% | |
| CATAATCTATATGTTTGGGA+GGG | + | chr1_2:76152667-76152686 | Msa0093400:CDS | 30.0% | |
| TCTGAGTAAACAAGATTGAT+CGG | - | chr1_2:76152799-76152818 | None:intergenic | 30.0% | |
| ! | CTTTAAGAACCAAACATCAC+TGG | - | chr1_2:76151003-76151022 | None:intergenic | 35.0% |
| AGGCTTGGAACAAAACATAA+AGG | - | chr1_2:76151026-76151045 | None:intergenic | 35.0% | |
| AAGTAAGTGATTGAAAGGCT+TGG | - | chr1_2:76151041-76151060 | None:intergenic | 35.0% | |
| ACATAGTATACAGGTCTCTT+TGG | - | chr1_2:76151519-76151538 | None:intergenic | 35.0% | |
| GGACTTTGTACATAGTATAC+AGG | - | chr1_2:76151528-76151547 | None:intergenic | 35.0% | |
| TCCAATATCAAACAGTCCTA+TGG | + | chr1_2:76151596-76151615 | Msa0093400:CDS | 35.0% | |
| TCCATAGGACTGTTTGATAT+TGG | - | chr1_2:76151600-76151619 | None:intergenic | 35.0% | |
| !!! | TTTTTAGGTCATCTTCAGGA+AGG | + | chr1_2:76151685-76151704 | Msa0093400:CDS | 35.0% |
| !! | TGTGGTGTTATACTTGAAGA+AGG | + | chr1_2:76151747-76151766 | Msa0093400:CDS | 35.0% |
| !! | GATTGCTGTGTTTACTTTTC+TGG | + | chr1_2:76151821-76151840 | Msa0093400:CDS | 35.0% |
| !! | CTTATTTCTACAAGCTCTCT+TGG | + | chr1_2:76152038-76152057 | Msa0093400:intron | 35.0% |
| TGATGCCTTCTTCAAAAAGA+TGG | - | chr1_2:76152230-76152249 | None:intergenic | 35.0% | |
| !!! | TACAACCCTGCTACATTTTT+TGG | + | chr1_2:76152754-76152773 | Msa0093400:CDS | 35.0% |
| TAAGAATGATCTTACCTCAC+CGG | - | chr1_2:76152836-76152855 | None:intergenic | 35.0% | |
| CCAAGTTCACTGATACAATA+CGG | - | chr1_2:76153014-76153033 | None:intergenic | 35.0% | |
| ATTGTATCAGTGAACTTGGA+AGG | + | chr1_2:76153015-76153034 | Msa0093400:CDS | 35.0% | |
| GGTAATAAGATTCCCCTTTA+TGG | + | chr1_2:76153036-76153055 | Msa0093400:CDS | 35.0% | |
| ACCATAACAGTTTCACAAAC+CGG | + | chr1_2:76153069-76153088 | Msa0093400:CDS | 35.0% | |
| TAAGGAAATCACTTGTCCTT+TGG | + | chr1_2:76153176-76153195 | Msa0093400:CDS | 35.0% | |
| !!! | GAGTATTTTTGAGATTGAGG+AGG | + | chr1_2:76153336-76153355 | Msa0093400:three_prime_UTR | 35.0% |
| TCAGAAACTTCTCTTGCAAA+TGG | - | chr1_2:76153363-76153382 | None:intergenic | 35.0% | |
| GTTTGCACTTTGCAAGGAAA+TGG | - | chr1_2:76151119-76151138 | None:intergenic | 40.0% | |
| ! | GAGTTTGTTTGCACTTTGCA+AGG | - | chr1_2:76151125-76151144 | None:intergenic | 40.0% |
| CCATCATGTGAATCTCTTGA+AGG | - | chr1_2:76151549-76151568 | None:intergenic | 40.0% | |
| CCTTCAAGAGATTCACATGA+TGG | + | chr1_2:76151546-76151565 | Msa0093400:CDS | 40.0% | |
| ! | TGATATTGGAGTAGCTTGCA+TGG | - | chr1_2:76151586-76151605 | None:intergenic | 40.0% |
| ! | TCTCCTTCACATCCTTCTTT+TGG | + | chr1_2:76151621-76151640 | Msa0093400:CDS | 40.0% |
| !!! | GGGATTTTTAGGTCATCTTC+AGG | + | chr1_2:76151681-76151700 | Msa0093400:CDS | 40.0% |
| GAAGGAAAGGTAGTAGAAAG+AGG | + | chr1_2:76151703-76151722 | Msa0093400:CDS | 40.0% | |
| GAAAGAGGAATGATAAAGGG+TGG | + | chr1_2:76151718-76151737 | Msa0093400:CDS | 40.0% | |
| TCAAGTATAACACCACACTC+AGG | - | chr1_2:76151744-76151763 | None:intergenic | 40.0% | |
| AGGTTCTTATCGTGAATTCG+AGG | + | chr1_2:76151767-76151786 | Msa0093400:CDS | 40.0% | |
| ACAGTAACTTCTGCCTTGAA+AGG | - | chr1_2:76151891-76151910 | None:intergenic | 40.0% | |
| !! | GTGGACCATCTTTTTGAAGA+AGG | + | chr1_2:76152222-76152241 | Msa0093400:intron | 40.0% |
| ATAGTAGGTGCAAGTGCAAA+TGG | - | chr1_2:76152298-76152317 | None:intergenic | 40.0% | |
| GGATGCCAAAAAATGTAGCA+GGG | - | chr1_2:76152762-76152781 | None:intergenic | 40.0% | |
| TGGATGCCAAAAAATGTAGC+AGG | - | chr1_2:76152763-76152782 | None:intergenic | 40.0% | |
| TCAGATATCAGTATCGCAAC+CGG | + | chr1_2:76152814-76152833 | Msa0093400:CDS | 40.0% | |
| ACAATACGGTGACTCTTTCT+TGG | - | chr1_2:76153000-76153019 | None:intergenic | 40.0% | |
| CCGTATTGTATCAGTGAACT+TGG | + | chr1_2:76153011-76153030 | Msa0093400:CDS | 40.0% | |
| ! | TCCGGTTTGTGAAACTGTTA+TGG | - | chr1_2:76153073-76153092 | None:intergenic | 40.0% |
| !! | AAGTGCTACTTCAACACTTC+CGG | - | chr1_2:76153091-76153110 | None:intergenic | 40.0% |
| ! | CCAGCAGAATTGATAACCAA+AGG | - | chr1_2:76153195-76153214 | None:intergenic | 40.0% |
| CCTTTGGTTATCAATTCTGC+TGG | + | chr1_2:76153192-76153211 | Msa0093400:CDS | 40.0% | |
| TTTCAGCAGCCAGTGATGTT+TGG | + | chr1_2:76150991-76151010 | Msa0093400:five_prime_UTR | 45.0% | |
| !! | GGAGATCTTGATGGTGAAGA+TGG | - | chr1_2:76151498-76151517 | None:intergenic | 45.0% |
| ! | GGTCTCTTTGGAGATCTTGA+TGG | - | chr1_2:76151507-76151526 | None:intergenic | 45.0% |
| CAGAAGAGTTTCTCGAGTGA+CGG | - | chr1_2:76151647-76151666 | None:intergenic | 45.0% | |
| CGAGAAACTCTTCTGCTAGT+CGG | + | chr1_2:76151652-76151671 | Msa0093400:CDS | 45.0% | |
| !! | CTCTTCTGCTAGTCGGTTTT+CGG | + | chr1_2:76151659-76151678 | Msa0093400:CDS | 45.0% |
| !! | TCTTCTGCTAGTCGGTTTTC+GGG | + | chr1_2:76151660-76151679 | Msa0093400:CDS | 45.0% |
| !! | GTCGGTTTTCGGGGATTTTT+AGG | + | chr1_2:76151670-76151689 | Msa0093400:CDS | 45.0% |
| AGGTCATCTTCAGGAAGGAA+AGG | + | chr1_2:76151690-76151709 | Msa0093400:CDS | 45.0% | |
| CAAGGCAGAAGTTACTGTCA+AGG | + | chr1_2:76151893-76151912 | Msa0093400:CDS | 45.0% | |
| ! | TTTTTGTTGGGACTCCTGTG+TGG | - | chr1_2:76152699-76152718 | None:intergenic | 45.0% |
| CAAGATTGATCGGTGAAGTG+TGG | - | chr1_2:76152789-76152808 | None:intergenic | 45.0% | |
| AAGATTCCCCTTTATGGAGC+TGG | + | chr1_2:76153042-76153061 | Msa0093400:CDS | 45.0% | |
| GTTGATCCAGCTCCATAAAG+GGG | - | chr1_2:76153051-76153070 | None:intergenic | 45.0% | |
| GGTTGATCCAGCTCCATAAA+GGG | - | chr1_2:76153052-76153071 | None:intergenic | 45.0% | |
| TGGTTGATCCAGCTCCATAA+AGG | - | chr1_2:76153053-76153072 | None:intergenic | 45.0% | |
| ! | AAGTTTGAAATCCGATCGCG+AGG | + | chr1_2:76153117-76153136 | Msa0093400:CDS | 45.0% |
| ! | GGTAAGGACAAGGCATCATA+AGG | + | chr1_2:76153158-76153177 | Msa0093400:CDS | 45.0% |
| ! | TCACTGGCTGCTGAAAGTGT+CGG | - | chr1_2:76150987-76151006 | None:intergenic | 50.0% |
| GGATGTGAAGGAGACTCCAT+AGG | - | chr1_2:76151615-76151634 | None:intergenic | 50.0% | |
| CGGCCAAAAGAAGGATGTGA+AGG | - | chr1_2:76151627-76151646 | None:intergenic | 50.0% | |
| !! | CTTCTGCTAGTCGGTTTTCG+GGG | + | chr1_2:76151661-76151680 | Msa0093400:CDS | 50.0% |
| ! | TGATCATTTGGGGTGCTAGC+AGG | + | chr1_2:76151865-76151884 | Msa0093400:CDS | 50.0% |
| TGATCGGTGAAGTGTGGACA+TGG | - | chr1_2:76152783-76152802 | None:intergenic | 50.0% | |
| TATCAGTATCGCAACCGGTG+AGG | + | chr1_2:76152819-76152838 | Msa0093400:CDS | 50.0% | |
| ! | ATGTGGTGGGGAAACTGGTA+AGG | + | chr1_2:76153142-76153161 | Msa0093400:CDS | 50.0% |
| TGGGGAAACTGGTAAGGACA+AGG | + | chr1_2:76153148-76153167 | Msa0093400:CDS | 50.0% | |
| CTCGAGTGACGGCCAAAAGA+AGG | - | chr1_2:76151636-76151655 | None:intergenic | 55.0% | |
| GATAAAGGGTGGCCTGAGTG+TGG | + | chr1_2:76151729-76151748 | Msa0093400:CDS | 55.0% | |
| AATCCGATCGCGAGGTGATG+TGG | + | chr1_2:76153125-76153144 | Msa0093400:CDS | 55.0% | |
| ! | AGGTGATGTGGTGGGGAAAC+TGG | + | chr1_2:76153137-76153156 | Msa0093400:CDS | 55.0% |
| GGGTGCTAGCAGGCCTTTCA+AGG | + | chr1_2:76151875-76151894 | Msa0093400:CDS | 60.0% | |
| ! | TGGGAGGGTACAGACCACAC+AGG | + | chr1_2:76152682-76152701 | Msa0093400:CDS | 60.0% |
| CCACCACATCACCTCGCGAT+CGG | - | chr1_2:76153131-76153150 | None:intergenic | 60.0% | |
| CGATCGCGAGGTGATGTGGT+GGG | + | chr1_2:76153129-76153148 | Msa0093400:CDS | 60.0% | |
| GATCGCGAGGTGATGTGGTG+GGG | + | chr1_2:76153130-76153149 | Msa0093400:CDS | 60.0% | |
| CCGATCGCGAGGTGATGTGG+TGG | + | chr1_2:76153128-76153147 | Msa0093400:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 76150974 | 76153511 | 76150974 | ID=Msa0093400;Name=Msa0093400 |
| chr1_2 | mRNA | 76150974 | 76153511 | 76150974 | ID=Msa0093400-mRNA-1;Parent=Msa0093400;Name=Msa0093400-mRNA-1;_AED=0.04;_eAED=0.04;_QI=479|1|1|1|1|1|3|251|309 |
| chr1_2 | exon | 76150974 | 76151914 | 76150974 | ID=Msa0093400-mRNA-1:exon:26806;Parent=Msa0093400-mRNA-1 |
| chr1_2 | exon | 76152655 | 76152840 | 76152655 | ID=Msa0093400-mRNA-1:exon:26807;Parent=Msa0093400-mRNA-1 |
| chr1_2 | exon | 76152979 | 76153511 | 76152979 | ID=Msa0093400-mRNA-1:exon:26808;Parent=Msa0093400-mRNA-1 |
| chr1_2 | five_prime_UTR | 76150974 | 76151452 | 76150974 | ID=Msa0093400-mRNA-1:five_prime_utr;Parent=Msa0093400-mRNA-1 |
| chr1_2 | CDS | 76151453 | 76151914 | 76151453 | ID=Msa0093400-mRNA-1:cds;Parent=Msa0093400-mRNA-1 |
| chr1_2 | CDS | 76152655 | 76152840 | 76152655 | ID=Msa0093400-mRNA-1:cds;Parent=Msa0093400-mRNA-1 |
| chr1_2 | CDS | 76152979 | 76153260 | 76152979 | ID=Msa0093400-mRNA-1:cds;Parent=Msa0093400-mRNA-1 |
| chr1_2 | three_prime_UTR | 76153261 | 76153511 | 76153261 | ID=Msa0093400-mRNA-1:three_prime_utr;Parent=Msa0093400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
