AlfalfaGEDB | Msa0353540

M. sativa cultivar zhongmu-4 / Msa0353540

KEGG: -
GO: GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016567 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0032392 GO:0032446 GO:0032508 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0070647 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363 GO:1901564 GO:1901576
PFAM: PF03478.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0353540	RHN73858.1	61.299	385	121	11	1	373	1	369	1.43e-144	424

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0353540	sp\|Q3EBZ2\|SKI23_ARATH	33.976	415	207	16	6	375	2	394	5.01e-51	178

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0353540	A0A396JBS2	61.299	385	121	11	1	373	1	369	6.81e-145	424

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0031520	Msa0353540	0.806175	9.343958e-50	-8.615850e-47
Msa0035150	Msa0353540	0.801003	1.105228e-48	-8.615850e-47
Msa0251640	Msa0353540	0.801571	8.455962e-49	-8.615850e-47
Msa0343850	Msa0353540	0.803376	3.590188e-49	-8.615850e-47
Msa0353540	Msa0372340	0.806173	9.352614e-50	-8.615850e-47
Msa0353540	Msa0402790	0.810137	1.338681e-50	-8.615850e-47
Msa0353540	Msa0512750	0.806792	6.927091e-50	-8.615850e-47
Msa0353540	Msa0523940	0.803411	3.530565e-49	-8.615850e-47
Msa0353540	Msa0529040	0.805383	1.370647e-49	-8.615850e-47
Msa0353540	Msa0539530	-0.808008	3.825414e-50	-8.615850e-47
Msa0353540	Msa0682680	0.800848	1.188562e-48	-8.615850e-47
Msa0353540	Msa0738250	0.802947	4.405054e-49	-8.615850e-47
Msa0353540	Msa0769980	0.825286	5.091227e-54	-8.615850e-47
Msa0353540	Msa0849030	0.806126	9.569022e-50	-8.615850e-47
Msa0353540	Msa0927380	0.808183	3.510923e-50	-8.615850e-47
Msa0353540	Msa0973100	0.802170	6.371585e-49	-8.615850e-47
Msa0353540	Msa1137570	0.811957	5.399549e-51	-8.615850e-47
Msa0353540	Msa1152100	0.800383	1.479108e-48	-8.615850e-47
Msa0353540	Msa1232740	0.815259	1.013402e-51	-8.615850e-47
Msa0353540	Msa1286800	0.806644	7.442607e-50	-8.615850e-47
Msa0353540	Msa1310400	0.800837	1.194894e-48	-8.615850e-47
Msa0353540	Msa1313320	0.823350	1.451957e-53	-8.615850e-47
Msa0353540	Msa1366970	0.807091	5.988428e-50	-8.615850e-47
Msa0353540	Msa1410690	0.807384	5.189469e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0353540	MtrunA17_Chr2g0303111	61.299	385	121	11	1	373	1	369	1.31e-148	424
Msa0353540	MtrunA17_Chr1g0146401	60.892	381	122	11	1	367	16	383	3.56e-142	409
Msa0353540	MtrunA17_Chr1g0157711	60.160	374	130	9	5	372	4	364	1.34e-136	394
Msa0353540	MtrunA17_Chr3g0088841	55.270	389	146	8	1	375	1	375	1.75e-136	394
Msa0353540	MtrunA17_Chr3g0088751	58.247	388	134	12	4	375	2	377	6.03e-135	390
Msa0353540	MtrunA17_Chr6g0457501	56.186	388	147	11	4	374	2	383	1.15e-130	380
Msa0353540	MtrunA17_Chr6g0457531	57.407	378	133	12	6	371	4	365	5.98e-128	372
Msa0353540	MtrunA17_Chr6g0460451	51.928	389	157	11	4	374	2	378	2.21e-121	355
Msa0353540	MtrunA17_Chr6g0460421	50.900	389	161	11	4	374	2	378	5.06e-118	347
Msa0353540	MtrunA17_Chr6g0457541	52.618	382	148	11	6	371	4	368	1.66e-116	343
Msa0353540	MtrunA17_Chr3g0088601	50.990	404	150	14	2	373	12	399	3.62e-111	330
Msa0353540	MtrunA17_Chr3g0088551	50.743	404	157	15	4	373	2	397	5.85e-111	330
Msa0353540	MtrunA17_Chr3g0088631	50.995	402	149	14	4	373	2	387	4.58e-109	325
Msa0353540	MtrunA17_Chr3g0088721	47.704	392	164	11	5	373	7	380	1.81e-106	318
Msa0353540	MtrunA17_Chr3g0127321	49.644	421	143	16	1	373	29	428	2.70e-106	319
Msa0353540	MtrunA17_Chr3g0088951	50.777	386	145	14	4	373	2	358	4.08e-105	313
Msa0353540	MtrunA17_Chr3g0089001	50.653	383	143	15	4	371	32	383	6.67e-103	309
Msa0353540	MtrunA17_Chr1g0151721	48.312	385	135	12	2	373	16	349	2.18e-101	304
Msa0353540	MtrunA17_Chr3g0088931	50.142	353	142	12	5	342	3	336	2.36e-98	297
Msa0353540	MtrunA17_Chr3g0127311	47.160	405	164	13	1	373	1	387	4.33e-98	297
Msa0353540	MtrunA17_Chr3g0088921	45.390	423	153	16	1	373	1	395	4.91e-96	291
Msa0353540	MtrunA17_Chr3g0088621	45.592	397	171	14	3	371	12	391	3.71e-93	284
Msa0353540	MtrunA17_Chr1g0170781	44.743	409	162	16	1	371	14	396	3.34e-90	277
Msa0353540	MtrunA17_Chr3g0088541	46.494	385	165	14	6	372	3	364	8.94e-90	275
Msa0353540	MtrunA17_Chr3g0088531	45.501	389	175	14	6	373	2	374	2.09e-89	274
Msa0353540	MtrunA17_Chr3g0089041	45.501	389	176	15	5	373	2	374	2.41e-89	274
Msa0353540	MtrunA17_Chr3g0088671	47.493	379	166	16	4	373	2	356	4.74e-87	267
Msa0353540	MtrunA17_Chr3g0088641	44.737	380	173	14	6	373	3	357	7.27e-86	264
Msa0353540	MtrunA17_Chr3g0088831	40.618	421	190	12	2	372	11	421	2.16e-85	265
Msa0353540	MtrunA17_Chr3g0089051	44.697	396	171	15	6	372	57	433	9.75e-85	264
Msa0353540	MtrunA17_Chr3g0088741	45.524	391	168	16	4	373	2	368	4.52e-84	260
Msa0353540	MtrunA17_Chr3g0088991	46.615	384	144	18	3	371	2	339	2.61e-83	257
Msa0353540	MtrunA17_Chr3g0088661	43.188	389	130	15	7	373	9	328	2.44e-75	236
Msa0353540	MtrunA17_Chr3g0088701	42.967	391	132	15	5	373	7	328	1.79e-74	234
Msa0353540	MtrunA17_Chr7g0214521	42.092	392	176	15	1	373	6	365	1.61e-72	230
Msa0353540	MtrunA17_Chr3g0096401	43.617	376	157	15	7	372	30	360	2.72e-68	219
Msa0353540	MtrunA17_Chr3g0088561	60.221	181	57	4	201	373	1	174	2.19e-61	195
Msa0353540	MtrunA17_Chr1g0146371	55.000	200	53	5	168	364	2	167	3.31e-56	181
Msa0353540	MtrunA17_Chr5g0417881	58.378	185	63	5	186	362	12	190	8.89e-55	179
Msa0353540	MtrunA17_Chr1g0151701	51.892	185	72	3	201	373	1	180	1.11e-50	167
Msa0353540	MtrunA17_Chr8g0392121	50.811	185	72	5	198	373	1	175	1.61e-48	162
Msa0353540	MtrunA17_Chr3g0088571	59.740	154	51	5	4	147	2	154	1.76e-46	156
Msa0353540	MtrunA17_Chr3g0092331	49.451	182	75	5	207	374	5	183	2.13e-45	154
Msa0353540	MtrunA17_Chr3g0088711	51.667	180	69	5	195	373	22	184	2.98e-45	154
Msa0353540	MtrunA17_Chr3g0088581	44.762	210	106	6	166	371	2	205	3.92e-44	151
Msa0353540	MtrunA17_Chr6g0457511	68.807	109	31	3	201	309	1	106	3.17e-40	138
Msa0353540	MtrunA17_Chr3g0088681	49.730	185	63	7	201	373	1	167	6.70e-39	136
Msa0353540	MtrunA17_Chr3g0088591	49.351	154	61	7	2	138	16	169	2.72e-35	127
Msa0353540	MtrunA17_Chr8g0371031	27.990	393	235	14	6	371	24	395	4.34e-34	130
Msa0353540	MtrunA17_Chr3g0088691	43.258	178	87	7	4	175	2	171	3.04e-31	117
Msa0353540	MtrunA17_Chr2g0300541	28.500	400	208	15	5	365	24	384	6.28e-31	121
Msa0353540	MtrunA17_Chr1g0146381	62.069	87	32	1	1	87	3	88	7.21e-29	107
Msa0353540	MtrunA17_Chr8g0371101	28.354	395	232	16	5	371	4	375	1.31e-28	114
Msa0353540	MtrunA17_Chr1g0146361	71.250	80	21	1	294	371	2	81	1.90e-27	103
Msa0353540	MtrunA17_Chr3g0088651	38.571	210	93	9	4	203	2	185	6.21e-27	105
Msa0353540	MtrunA17_Chr6g0457521	52.885	104	41	3	1	97	52	154	1.98e-25	101
Msa0353540	MtrunA17_Chr5g0435201	26.331	338	210	11	6	329	5	317	5.68e-24	101
Msa0353540	MtrunA17_Chr4g0010441	50.588	85	37	1	293	372	2	86	2.80e-20	84.7
Msa0353540	MtrunA17_Chr8g0351561	52.475	101	37	5	210	308	1	92	8.96e-20	85.1
Msa0353540	MtrunA17_Chr6g0488491	23.739	337	226	8	1	329	2	315	1.21e-18	86.3
Msa0353540	MtrunA17_Chr3g0101901	40.940	149	59	8	7	144	26	156	1.53e-18	82.0
Msa0353540	MtrunA17_Chr8g0349911	63.077	65	22	1	156	220	86	148	4.56e-18	81.6
Msa0353540	MtrunA17_Chr6g0449121	59.740	77	22	2	249	325	2	69	1.30e-17	76.6
Msa0353540	MtrunA17_Chr3g0143621	25.564	399	251	16	6	372	22	406	5.89e-15	75.9
Msa0353540	MtrunA17_Chr6g0457551	70.000	40	12	0	4	43	2	41	2.87e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0353540	AT2G17030.1	33.976	415	207	16	6	375	2	394	5.09e-52	178
Msa0353540	AT2G17036.2	33.418	392	226	15	7	372	2	384	4.97e-50	173
Msa0353540	AT2G17036.1	33.418	392	226	15	7	372	3	385	5.83e-50	173
Msa0353540	AT5G24040.1	25.521	384	248	11	8	371	4	369	2.68e-22	97.4
Msa0353540	AT5G60060.1	28.402	338	204	15	6	322	9	329	1.61e-20	92.4
Msa0353540	AT2G26160.1	24.363	353	208	12	7	335	5	322	6.32e-16	78.6
Msa0353540	AT1G65770.1	25.895	363	241	15	6	358	2	346	8.72e-16	78.2
Msa0353540	AT3G25750.2	26.113	337	197	13	5	320	3	308	1.88e-14	74.3

Find 104 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTAGAATATTCAGGATATT+TGG	0.162233	3_1:-23653761	None:intergenic
TCTTGCAAGTATTAATTTCT+TGG	0.210109	3_1:+23653516	Msa0353540:CDS
GAGGGAGAATCTGTTAAAAT+TGG	0.249452	3_1:-23653080	None:intergenic
TTTCAATGGGCGGCCTTATC+TGG	0.262250	3_1:+23653327	Msa0353540:CDS
CATAACAATTTAGAATATTC+AGG	0.267633	3_1:-23653769	None:intergenic
GGGTGAAGTTGGCGAATTTA+GGG	0.273559	3_1:+23653581	Msa0353540:CDS
TGAAATGCGGCTGTGATAAT+TGG	0.282252	3_1:+23653260	Msa0353540:CDS
AAAATGTTACAACGGTGATT+TGG	0.286056	3_1:-23652830	None:intergenic
GTGGCTGAACCTTTGGTTGA+TGG	0.316763	3_1:+23653406	Msa0353540:CDS
AAAGGTTTGGGTGGGAGATA+TGG	0.318929	3_1:-23653040	None:intergenic
TGGGTGAAGTTGGCGAATTT+AGG	0.324049	3_1:+23653580	Msa0353540:CDS
TTCGATCAATTTGTTCCAAT+TGG	0.330753	3_1:+23652792	Msa0353540:CDS
AAATGTTACAACGGTGATTT+GGG	0.340210	3_1:-23652829	None:intergenic
CTGAATATTCTAAATTGTTA+TGG	0.344183	3_1:+23653770	Msa0353540:CDS
AGGGAATTGTGTGATCATTA+TGG	0.349524	3_1:+23653672	Msa0353540:CDS
CAGATTCTCCCTCCTTCATT+TGG	0.350995	3_1:+23653090	Msa0353540:CDS
CCAGTTAGTGGCTGAACCTT+TGG	0.355761	3_1:+23653399	Msa0353540:CDS
TCCTGACATGTCGCCTTGCT+CGG	0.359645	3_1:+23653291	Msa0353540:CDS
CCAAAGGTTCAGCCACTAAC+TGG	0.369613	3_1:-23653399	None:intergenic
GGAGAAGAAGTGGGTGAAGT+TGG	0.400739	3_1:+23653570	Msa0353540:CDS
GATAATATATCCACTTTATG+TGG	0.411499	3_1:+23653694	Msa0353540:CDS
TGTGTTTAAGCTTAATGAAA+AGG	0.411651	3_1:+23653549	Msa0353540:CDS
CCTTATCTGGTAGACAAAAC+TGG	0.413089	3_1:+23653340	Msa0353540:CDS
GATGAGGAAGAAACTGTGTT+TGG	0.423904	3_1:-23652930	None:intergenic
ATCAGATGAAGAAGATCTTT+CGG	0.425766	3_1:-23652731	None:intergenic
GGCACAGGATTGAAAGGAAA+AGG	0.428670	3_1:-23653058	None:intergenic
AGGATTGAAAGGAAAAGGTT+TGG	0.428997	3_1:-23653053	None:intergenic
TGGCGGGAAAAGAAAATTCT+TGG	0.433576	3_1:+23653429	Msa0353540:CDS
GGATTGAAAGGAAAAGGTTT+GGG	0.436408	3_1:-23653052	None:intergenic
CAGCCGCATTTCAAGAGTGC+TGG	0.443260	3_1:-23653250	None:intergenic
TCAACCAAGGACGGTGAACT+AGG	0.444951	3_1:-23652973	None:intergenic
AAACCCTAGTTCACCGTCCT+TGG	0.446765	3_1:+23652969	Msa0353540:CDS
ACTAGGGTTTGTTGTGGTGG+TGG	0.450063	3_1:-23652956	None:intergenic
CTAAGCGTACCGAGTATGAT+AGG	0.451050	3_1:-23653208	None:intergenic
GAATGAAGTATTGTTGTTGA+GGG	0.456327	3_1:-23652897	None:intergenic
CCCGAGCAAGGCGACATGTC+AGG	0.457783	3_1:-23653292	None:intergenic
GAACCTTTGGTTGATGGTGG+CGG	0.460372	3_1:+23653412	Msa0353540:CDS
GTCTGCCTCCGCCGTCGCCA+TGG	0.460636	3_1:-23652696	None:intergenic
AAAACAGCTCCCACATAAAG+TGG	0.468733	3_1:-23653704	None:intergenic
GAGATATGGATGTAAGAGTT+GGG	0.470914	3_1:-23653026	None:intergenic
TGGCGACGGCGGAGGCAGAC+TGG	0.472179	3_1:+23652699	Msa0353540:CDS
CCTGACATGTCGCCTTGCTC+GGG	0.472811	3_1:+23653292	Msa0353540:CDS
CAGATTTGTGTGTTGCCAAA+GGG	0.473760	3_1:+23653653	Msa0353540:CDS
CAAGTATTAATTTCTTGGTA+CGG	0.473932	3_1:+23653521	Msa0353540:CDS
AATGATCACACAATTCCCTT+TGG	0.477126	3_1:-23653668	None:intergenic
AGGGAGAATCTGTTAAAATT+GGG	0.477711	3_1:-23653079	None:intergenic
TTGGCGATCCACTCTGGAGG+TGG	0.481012	3_1:-23653793	None:intergenic
GATAAGGCCGCCCATTGAAA+AGG	0.491553	3_1:-23653324	None:intergenic
TCGAATGGGTATCTGAGTAG+TGG	0.491826	3_1:-23652866	None:intergenic
AACCTTTGGTTGATGGTGGC+GGG	0.492093	3_1:+23653413	Msa0353540:CDS
TTCATCCATGGCGACGGCGG+AGG	0.494300	3_1:+23652691	None:intergenic
CGACTCCACTGTCCAGTTAG+TGG	0.503077	3_1:+23653387	Msa0353540:CDS
AGCTTCATCCATGGCGACGG+CGG	0.503486	3_1:+23652688	None:intergenic
AGAATGAAGTATTGTTGTTG+AGG	0.504807	3_1:-23652898	None:intergenic
GATTGAGGAAGAGCGCCAAT+TGG	0.508882	3_1:-23652807	None:intergenic
GGAGATATGGATGTAAGAGT+TGG	0.511354	3_1:-23653027	None:intergenic
TATGAGTCTTTAGAGTTCGA+TGG	0.511537	3_1:+23653487	Msa0353540:CDS
AAAATTCTTGGTGGAGAGTG+AGG	0.513659	3_1:+23653441	Msa0353540:CDS
TGAGGGAATCAATTTCGAAT+GGG	0.514429	3_1:-23652880	None:intergenic
AACTGGACAGTGGAGTCGTC+CGG	0.522830	3_1:-23653382	None:intergenic
TTGAGGGAATCAATTTCGAA+TGG	0.524973	3_1:-23652881	None:intergenic
ATCTGAGTAGTGGGTACTCG+AGG	0.526011	3_1:-23652856	None:intergenic
ACAACGGTGATTTGGGATTG+AGG	0.530478	3_1:-23652822	None:intergenic
TGAAGTTAGTTACCAAATGA+AGG	0.536638	3_1:-23653102	None:intergenic
TCATCTGATATCACAACGAA+TGG	0.553104	3_1:+23652745	Msa0353540:CDS
TTAATGAAAAGGAGAAGAAG+TGG	0.562031	3_1:+23653560	Msa0353540:CDS
GTTGTGGTGGTGGTTTGATG+AGG	0.562035	3_1:-23652946	None:intergenic
GTACATTTCGGTATAGTGCA+AGG	0.562073	3_1:-23653146	None:intergenic
AAATTGGGCACAGGATTGAA+AGG	0.564433	3_1:-23653064	None:intergenic
CGAATGGGTATCTGAGTAGT+GGG	0.565433	3_1:-23652865	None:intergenic
CGGGAAAAGAAAATTCTTGG+TGG	0.567651	3_1:+23653432	Msa0353540:CDS
TGAAAGGAAAAGGTTTGGGT+GGG	0.569335	3_1:-23653048	None:intergenic
TTCCCGCCACCATCAACCAA+AGG	0.571866	3_1:-23653415	None:intergenic
TGAACTAGGGTTTGTTGTGG+TGG	0.571982	3_1:-23652959	None:intergenic
AATCTGTTAAAATTGGGCAC+AGG	0.577223	3_1:-23653073	None:intergenic
TACATTTCGGTATAGTGCAA+GGG	0.578026	3_1:-23653145	None:intergenic
AGGCAGATGTCTCCCGAGCA+AGG	0.579393	3_1:-23653304	None:intergenic
CAACCAAGGACGGTGAACTA+GGG	0.579911	3_1:-23652972	None:intergenic
AAATTCTTGGTGGAGAGTGA+GGG	0.579999	3_1:+23653442	Msa0353540:CDS
ACTGGAAGGACGGTTACTGT+TGG	0.582168	3_1:+23653358	Msa0353540:CDS
TAATGAAAAGGAGAAGAAGT+GGG	0.584827	3_1:+23653561	Msa0353540:CDS
TTGAAAGGAAAAGGTTTGGG+TGG	0.586561	3_1:-23653049	None:intergenic
ATAATATATCCACTTTATGT+GGG	0.595924	3_1:+23653695	Msa0353540:CDS
TCAGATTTGTGTGTTGCCAA+AGG	0.597712	3_1:+23653652	Msa0353540:CDS
GGAAGAAACTGTGTTTGGAA+AGG	0.598979	3_1:-23652925	None:intergenic
CGGTGAACTAGGGTTTGTTG+TGG	0.608078	3_1:-23652962	None:intergenic
GGTGAAGTTGGCGAATTTAG+GGG	0.608400	3_1:+23653582	Msa0353540:CDS
AAGGACGGTTACTGTTGGAC+CGG	0.609138	3_1:+23653363	Msa0353540:CDS
AGAATTACCCAAAATTCTAG+TGG	0.610467	3_1:+23652998	Msa0353540:CDS
AGTTAGTTACCAAATGAAGG+AGG	0.618591	3_1:-23653099	None:intergenic
TGGGTAATTCTGATCAACCA+AGG	0.622582	3_1:-23652986	None:intergenic
AACAGCTTCATCCATGGCGA+CGG	0.626295	3_1:+23652685	None:intergenic
GTTAGTTACCAAATGAAGGA+GGG	0.627616	3_1:-23653098	None:intergenic
TGAGGGTGAGTTATTGCTAA+CGG	0.631275	3_1:+23653459	Msa0353540:CDS
GCTGAACCTTTGGTTGATGG+TGG	0.633853	3_1:+23653409	Msa0353540:CDS
TAATTCTGATCAACCAAGGA+CGG	0.636208	3_1:-23652982	None:intergenic
GGTAGACAAAACTGGAAGGA+CGG	0.644700	3_1:+23653348	Msa0353540:CDS
TCTGAGTAGTGGGTACTCGA+GGG	0.648300	3_1:-23652855	None:intergenic
ATCTGGTAGACAAAACTGGA+AGG	0.649259	3_1:+23653344	Msa0353540:CDS
AAACCAGCACTCTTGAAATG+CGG	0.661445	3_1:+23653247	Msa0353540:CDS
ATGCGGCTGTGATAATTGGA+AGG	0.663891	3_1:+23653264	Msa0353540:CDS
TGTTATGGCCACCTCCAGAG+TGG	0.665853	3_1:+23653785	Msa0353540:CDS
TTCAGCCACTAACTGGACAG+TGG	0.686028	3_1:-23653392	None:intergenic
CGAGGGTTAAAATGTTACAA+CGG	0.694522	3_1:-23652838	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATATATCCACTTTATGT+GGG	+	chr3_1:23653695-23653714	Msa0353540:CDS	20.0%
!!!	TTTAGAATATTCAGGATATT+TGG	-	chr3_1:23653764-23653783	None:intergenic	20.0%
!!!	CATAACAATTTAGAATATTC+AGG	-	chr3_1:23653772-23653791	None:intergenic	20.0%
!!	CTGAATATTCTAAATTGTTA+TGG	+	chr3_1:23653770-23653789	Msa0353540:CDS	20.0%
!!	ATAATATATCCACTTTATGT+GGG	+	chr3_1:23653695-23653714	Msa0353540:CDS	20.0%
!!!	TTTAGAATATTCAGGATATT+TGG	-	chr3_1:23653764-23653783	None:intergenic	20.0%
!!!	CATAACAATTTAGAATATTC+AGG	-	chr3_1:23653772-23653791	None:intergenic	20.0%
!!	CTGAATATTCTAAATTGTTA+TGG	+	chr3_1:23653770-23653789	Msa0353540:CDS	20.0%
!	TCTTGCAAGTATTAATTTCT+TGG	+	chr3_1:23653516-23653535	Msa0353540:CDS	25.0%
!	CAAGTATTAATTTCTTGGTA+CGG	+	chr3_1:23653521-23653540	Msa0353540:CDS	25.0%
!	TGTGTTTAAGCTTAATGAAA+AGG	+	chr3_1:23653549-23653568	Msa0353540:CDS	25.0%
!	GATAATATATCCACTTTATG+TGG	+	chr3_1:23653694-23653713	Msa0353540:CDS	25.0%
!	TCTTGCAAGTATTAATTTCT+TGG	+	chr3_1:23653516-23653535	Msa0353540:CDS	25.0%
!	CAAGTATTAATTTCTTGGTA+CGG	+	chr3_1:23653521-23653540	Msa0353540:CDS	25.0%
!	TGTGTTTAAGCTTAATGAAA+AGG	+	chr3_1:23653549-23653568	Msa0353540:CDS	25.0%
!	GATAATATATCCACTTTATG+TGG	+	chr3_1:23653694-23653713	Msa0353540:CDS	25.0%
	ATCAGATGAAGAAGATCTTT+CGG	-	chr3_1:23652734-23652753	None:intergenic	30.0%
!	TTCGATCAATTTGTTCCAAT+TGG	+	chr3_1:23652792-23652811	Msa0353540:CDS	30.0%
	AAATGTTACAACGGTGATTT+GGG	-	chr3_1:23652832-23652851	None:intergenic	30.0%
	AAAATGTTACAACGGTGATT+TGG	-	chr3_1:23652833-23652852	None:intergenic	30.0%
!!	GAATGAAGTATTGTTGTTGA+GGG	-	chr3_1:23652900-23652919	None:intergenic	30.0%
!!	AGAATGAAGTATTGTTGTTG+AGG	-	chr3_1:23652901-23652920	None:intergenic	30.0%
	AGAATTACCCAAAATTCTAG+TGG	+	chr3_1:23652998-23653017	Msa0353540:CDS	30.0%
	AGGGAGAATCTGTTAAAATT+GGG	-	chr3_1:23653082-23653101	None:intergenic	30.0%
	TGAAGTTAGTTACCAAATGA+AGG	-	chr3_1:23653105-23653124	None:intergenic	30.0%
	GACAAAAAACCTATCATACT+CGG	+	chr3_1:23653199-23653218	Msa0353540:CDS	30.0%
	TTAATGAAAAGGAGAAGAAG+TGG	+	chr3_1:23653560-23653579	Msa0353540:CDS	30.0%
	TAATGAAAAGGAGAAGAAGT+GGG	+	chr3_1:23653561-23653580	Msa0353540:CDS	30.0%
	ATCAGATGAAGAAGATCTTT+CGG	-	chr3_1:23652734-23652753	None:intergenic	30.0%
!	TTCGATCAATTTGTTCCAAT+TGG	+	chr3_1:23652792-23652811	Msa0353540:CDS	30.0%
	AAATGTTACAACGGTGATTT+GGG	-	chr3_1:23652832-23652851	None:intergenic	30.0%
	AAAATGTTACAACGGTGATT+TGG	-	chr3_1:23652833-23652852	None:intergenic	30.0%
!!	GAATGAAGTATTGTTGTTGA+GGG	-	chr3_1:23652900-23652919	None:intergenic	30.0%
!!	AGAATGAAGTATTGTTGTTG+AGG	-	chr3_1:23652901-23652920	None:intergenic	30.0%
	AGAATTACCCAAAATTCTAG+TGG	+	chr3_1:23652998-23653017	Msa0353540:CDS	30.0%
	AGGGAGAATCTGTTAAAATT+GGG	-	chr3_1:23653082-23653101	None:intergenic	30.0%
	TGAAGTTAGTTACCAAATGA+AGG	-	chr3_1:23653105-23653124	None:intergenic	30.0%
	GACAAAAAACCTATCATACT+CGG	+	chr3_1:23653199-23653218	Msa0353540:CDS	30.0%
	TTAATGAAAAGGAGAAGAAG+TGG	+	chr3_1:23653560-23653579	Msa0353540:CDS	30.0%
	TAATGAAAAGGAGAAGAAGT+GGG	+	chr3_1:23653561-23653580	Msa0353540:CDS	30.0%
	TCATCTGATATCACAACGAA+TGG	+	chr3_1:23652745-23652764	Msa0353540:CDS	35.0%
	CGAGGGTTAAAATGTTACAA+CGG	-	chr3_1:23652841-23652860	None:intergenic	35.0%
	TGAGGGAATCAATTTCGAAT+GGG	-	chr3_1:23652883-23652902	None:intergenic	35.0%
	TTGAGGGAATCAATTTCGAA+TGG	-	chr3_1:23652884-23652903	None:intergenic	35.0%
	TAATTCTGATCAACCAAGGA+CGG	-	chr3_1:23652985-23653004	None:intergenic	35.0%
!	GGTTTTGCCACTAGAATTTT+GGG	-	chr3_1:23653008-23653027	None:intergenic	35.0%
	GAGATATGGATGTAAGAGTT+GGG	-	chr3_1:23653029-23653048	None:intergenic	35.0%
	GGATTGAAAGGAAAAGGTTT+GGG	-	chr3_1:23653055-23653074	None:intergenic	35.0%
	AGGATTGAAAGGAAAAGGTT+TGG	-	chr3_1:23653056-23653075	None:intergenic	35.0%
	AATCTGTTAAAATTGGGCAC+AGG	-	chr3_1:23653076-23653095	None:intergenic	35.0%
	GAGGGAGAATCTGTTAAAAT+TGG	-	chr3_1:23653083-23653102	None:intergenic	35.0%
	GTTAGTTACCAAATGAAGGA+GGG	-	chr3_1:23653101-23653120	None:intergenic	35.0%
	AGTTAGTTACCAAATGAAGG+AGG	-	chr3_1:23653102-23653121	None:intergenic	35.0%
	TACATTTCGGTATAGTGCAA+GGG	-	chr3_1:23653148-23653167	None:intergenic	35.0%
!!!	GACTTTTTCAGGGTACATTT+CGG	-	chr3_1:23653161-23653180	None:intergenic	35.0%
!!	TATGAGTCTTTAGAGTTCGA+TGG	+	chr3_1:23653487-23653506	Msa0353540:CDS	35.0%
!!!	AGGGGATAGAGTTTTGTTTT+TGG	+	chr3_1:23653600-23653619	Msa0353540:CDS	35.0%
!!!	GGGGATAGAGTTTTGTTTTT+GGG	+	chr3_1:23653601-23653620	Msa0353540:CDS	35.0%
	AATGATCACACAATTCCCTT+TGG	-	chr3_1:23653671-23653690	None:intergenic	35.0%
	AGGGAATTGTGTGATCATTA+TGG	+	chr3_1:23653672-23653691	Msa0353540:CDS	35.0%
!!!	TTTATGTGGGAGCTGTTTTT+TGG	+	chr3_1:23653708-23653727	Msa0353540:CDS	35.0%
!!!	TGTTTTTTGGACTTGGATGA+TGG	+	chr3_1:23653721-23653740	Msa0353540:CDS	35.0%
	TCATCTGATATCACAACGAA+TGG	+	chr3_1:23652745-23652764	Msa0353540:CDS	35.0%
	CGAGGGTTAAAATGTTACAA+CGG	-	chr3_1:23652841-23652860	None:intergenic	35.0%
	TGAGGGAATCAATTTCGAAT+GGG	-	chr3_1:23652883-23652902	None:intergenic	35.0%
	TTGAGGGAATCAATTTCGAA+TGG	-	chr3_1:23652884-23652903	None:intergenic	35.0%
	TAATTCTGATCAACCAAGGA+CGG	-	chr3_1:23652985-23653004	None:intergenic	35.0%
!	GGTTTTGCCACTAGAATTTT+GGG	-	chr3_1:23653008-23653027	None:intergenic	35.0%
	GAGATATGGATGTAAGAGTT+GGG	-	chr3_1:23653029-23653048	None:intergenic	35.0%
	GGATTGAAAGGAAAAGGTTT+GGG	-	chr3_1:23653055-23653074	None:intergenic	35.0%
	AGGATTGAAAGGAAAAGGTT+TGG	-	chr3_1:23653056-23653075	None:intergenic	35.0%
	AATCTGTTAAAATTGGGCAC+AGG	-	chr3_1:23653076-23653095	None:intergenic	35.0%
	GAGGGAGAATCTGTTAAAAT+TGG	-	chr3_1:23653083-23653102	None:intergenic	35.0%
	GTTAGTTACCAAATGAAGGA+GGG	-	chr3_1:23653101-23653120	None:intergenic	35.0%
	AGTTAGTTACCAAATGAAGG+AGG	-	chr3_1:23653102-23653121	None:intergenic	35.0%
	TACATTTCGGTATAGTGCAA+GGG	-	chr3_1:23653148-23653167	None:intergenic	35.0%
!!!	GACTTTTTCAGGGTACATTT+CGG	-	chr3_1:23653161-23653180	None:intergenic	35.0%
!!	TATGAGTCTTTAGAGTTCGA+TGG	+	chr3_1:23653487-23653506	Msa0353540:CDS	35.0%
!!!	AGGGGATAGAGTTTTGTTTT+TGG	+	chr3_1:23653600-23653619	Msa0353540:CDS	35.0%
!!!	GGGGATAGAGTTTTGTTTTT+GGG	+	chr3_1:23653601-23653620	Msa0353540:CDS	35.0%
	AATGATCACACAATTCCCTT+TGG	-	chr3_1:23653671-23653690	None:intergenic	35.0%
	AGGGAATTGTGTGATCATTA+TGG	+	chr3_1:23653672-23653691	Msa0353540:CDS	35.0%
!!!	TTTATGTGGGAGCTGTTTTT+TGG	+	chr3_1:23653708-23653727	Msa0353540:CDS	35.0%
!!!	TGTTTTTTGGACTTGGATGA+TGG	+	chr3_1:23653721-23653740	Msa0353540:CDS	35.0%
	GGAAGAAACTGTGTTTGGAA+AGG	-	chr3_1:23652928-23652947	None:intergenic	40.0%
	GATGAGGAAGAAACTGTGTT+TGG	-	chr3_1:23652933-23652952	None:intergenic	40.0%
	TGGGTAATTCTGATCAACCA+AGG	-	chr3_1:23652989-23653008	None:intergenic	40.0%
!	GGGTTTTGCCACTAGAATTT+TGG	-	chr3_1:23653009-23653028	None:intergenic	40.0%
	GGAGATATGGATGTAAGAGT+TGG	-	chr3_1:23653030-23653049	None:intergenic	40.0%
	TGAAAGGAAAAGGTTTGGGT+GGG	-	chr3_1:23653051-23653070	None:intergenic	40.0%
	TTGAAAGGAAAAGGTTTGGG+TGG	-	chr3_1:23653052-23653071	None:intergenic	40.0%
	AAATTGGGCACAGGATTGAA+AGG	-	chr3_1:23653067-23653086	None:intergenic	40.0%
!!!	TGCAAGGGTTCTTTTTTGTC+GGG	-	chr3_1:23653133-23653152	None:intergenic	40.0%
!!!	GTGCAAGGGTTCTTTTTTGT+CGG	-	chr3_1:23653134-23653153	None:intergenic	40.0%
	GTACATTTCGGTATAGTGCA+AGG	-	chr3_1:23653149-23653168	None:intergenic	40.0%
!!!	TGACAGCTACGACTTTTTCA+GGG	-	chr3_1:23653171-23653190	None:intergenic	40.0%
!!!	TTTCAAGAGTGCTGGTTTTG+AGG	-	chr3_1:23653245-23653264	None:intergenic	40.0%
	AAACCAGCACTCTTGAAATG+CGG	+	chr3_1:23653247-23653266	Msa0353540:CDS	40.0%
	TGAAATGCGGCTGTGATAAT+TGG	+	chr3_1:23653260-23653279	Msa0353540:CDS	40.0%
!	GAGACATCTGCCTTTTCAAT+GGG	+	chr3_1:23653314-23653333	Msa0353540:CDS	40.0%
!!	CCAGTTTTGTCTACCAGATA+AGG	-	chr3_1:23653343-23653362	None:intergenic	40.0%
	CCTTATCTGGTAGACAAAAC+TGG	+	chr3_1:23653340-23653359	Msa0353540:CDS	40.0%
	ATCTGGTAGACAAAACTGGA+AGG	+	chr3_1:23653344-23653363	Msa0353540:CDS	40.0%
	TGGCGGGAAAAGAAAATTCT+TGG	+	chr3_1:23653429-23653448	Msa0353540:CDS	40.0%
	CGGGAAAAGAAAATTCTTGG+TGG	+	chr3_1:23653432-23653451	Msa0353540:CDS	40.0%
	AAAATTCTTGGTGGAGAGTG+AGG	+	chr3_1:23653441-23653460	Msa0353540:CDS	40.0%
	AAATTCTTGGTGGAGAGTGA+GGG	+	chr3_1:23653442-23653461	Msa0353540:CDS	40.0%
	TGAGGGTGAGTTATTGCTAA+CGG	+	chr3_1:23653459-23653478	Msa0353540:CDS	40.0%
	TCAGATTTGTGTGTTGCCAA+AGG	+	chr3_1:23653652-23653671	Msa0353540:CDS	40.0%
	CAGATTTGTGTGTTGCCAAA+GGG	+	chr3_1:23653653-23653672	Msa0353540:CDS	40.0%
	AAAACAGCTCCCACATAAAG+TGG	-	chr3_1:23653707-23653726	None:intergenic	40.0%
	GGAAGAAACTGTGTTTGGAA+AGG	-	chr3_1:23652928-23652947	None:intergenic	40.0%
	GATGAGGAAGAAACTGTGTT+TGG	-	chr3_1:23652933-23652952	None:intergenic	40.0%
	TGGGTAATTCTGATCAACCA+AGG	-	chr3_1:23652989-23653008	None:intergenic	40.0%
!	GGGTTTTGCCACTAGAATTT+TGG	-	chr3_1:23653009-23653028	None:intergenic	40.0%
	GGAGATATGGATGTAAGAGT+TGG	-	chr3_1:23653030-23653049	None:intergenic	40.0%
	TGAAAGGAAAAGGTTTGGGT+GGG	-	chr3_1:23653051-23653070	None:intergenic	40.0%
	TTGAAAGGAAAAGGTTTGGG+TGG	-	chr3_1:23653052-23653071	None:intergenic	40.0%
	AAATTGGGCACAGGATTGAA+AGG	-	chr3_1:23653067-23653086	None:intergenic	40.0%
!!!	TGCAAGGGTTCTTTTTTGTC+GGG	-	chr3_1:23653133-23653152	None:intergenic	40.0%
!!!	GTGCAAGGGTTCTTTTTTGT+CGG	-	chr3_1:23653134-23653153	None:intergenic	40.0%
	GTACATTTCGGTATAGTGCA+AGG	-	chr3_1:23653149-23653168	None:intergenic	40.0%
!!!	TGACAGCTACGACTTTTTCA+GGG	-	chr3_1:23653171-23653190	None:intergenic	40.0%
!!!	TTTCAAGAGTGCTGGTTTTG+AGG	-	chr3_1:23653245-23653264	None:intergenic	40.0%
	AAACCAGCACTCTTGAAATG+CGG	+	chr3_1:23653247-23653266	Msa0353540:CDS	40.0%
	TGAAATGCGGCTGTGATAAT+TGG	+	chr3_1:23653260-23653279	Msa0353540:CDS	40.0%
!	GAGACATCTGCCTTTTCAAT+GGG	+	chr3_1:23653314-23653333	Msa0353540:CDS	40.0%
!!	CCAGTTTTGTCTACCAGATA+AGG	-	chr3_1:23653343-23653362	None:intergenic	40.0%
	CCTTATCTGGTAGACAAAAC+TGG	+	chr3_1:23653340-23653359	Msa0353540:CDS	40.0%
	ATCTGGTAGACAAAACTGGA+AGG	+	chr3_1:23653344-23653363	Msa0353540:CDS	40.0%
	TGGCGGGAAAAGAAAATTCT+TGG	+	chr3_1:23653429-23653448	Msa0353540:CDS	40.0%
	CGGGAAAAGAAAATTCTTGG+TGG	+	chr3_1:23653432-23653451	Msa0353540:CDS	40.0%
	AAAATTCTTGGTGGAGAGTG+AGG	+	chr3_1:23653441-23653460	Msa0353540:CDS	40.0%
	AAATTCTTGGTGGAGAGTGA+GGG	+	chr3_1:23653442-23653461	Msa0353540:CDS	40.0%
	TGAGGGTGAGTTATTGCTAA+CGG	+	chr3_1:23653459-23653478	Msa0353540:CDS	40.0%
	TCAGATTTGTGTGTTGCCAA+AGG	+	chr3_1:23653652-23653671	Msa0353540:CDS	40.0%
	CAGATTTGTGTGTTGCCAAA+GGG	+	chr3_1:23653653-23653672	Msa0353540:CDS	40.0%
	AAAACAGCTCCCACATAAAG+TGG	-	chr3_1:23653707-23653726	None:intergenic	40.0%
!	ACAACGGTGATTTGGGATTG+AGG	-	chr3_1:23652825-23652844	None:intergenic	45.0%
	CGAATGGGTATCTGAGTAGT+GGG	-	chr3_1:23652868-23652887	None:intergenic	45.0%
	TCGAATGGGTATCTGAGTAG+TGG	-	chr3_1:23652869-23652888	None:intergenic	45.0%
!!	TGAACTAGGGTTTGTTGTGG+TGG	-	chr3_1:23652962-23652981	None:intergenic	45.0%
	AAAGGTTTGGGTGGGAGATA+TGG	-	chr3_1:23653043-23653062	None:intergenic	45.0%
	GGCACAGGATTGAAAGGAAA+AGG	-	chr3_1:23653061-23653080	None:intergenic	45.0%
	CAGATTCTCCCTCCTTCATT+TGG	+	chr3_1:23653090-23653109	Msa0353540:CDS	45.0%
!!	GTGACAGCTACGACTTTTTC+AGG	-	chr3_1:23653172-23653191	None:intergenic	45.0%
	CTAAGCGTACCGAGTATGAT+AGG	-	chr3_1:23653211-23653230	None:intergenic	45.0%
!!!	AGAGTGCTGGTTTTGAGGTA+CGG	-	chr3_1:23653240-23653259	None:intergenic	45.0%
	ATGCGGCTGTGATAATTGGA+AGG	+	chr3_1:23653264-23653283	Msa0353540:CDS	45.0%
!	GGAGACATCTGCCTTTTCAA+TGG	+	chr3_1:23653313-23653332	Msa0353540:CDS	45.0%
!	ACATCTGCCTTTTCAATGGG+CGG	+	chr3_1:23653317-23653336	Msa0353540:CDS	45.0%
	GGTAGACAAAACTGGAAGGA+CGG	+	chr3_1:23653348-23653367	Msa0353540:CDS	45.0%
	TGGGTGAAGTTGGCGAATTT+AGG	+	chr3_1:23653580-23653599	Msa0353540:CDS	45.0%
	GGGTGAAGTTGGCGAATTTA+GGG	+	chr3_1:23653581-23653600	Msa0353540:CDS	45.0%
	GGTGAAGTTGGCGAATTTAG+GGG	+	chr3_1:23653582-23653601	Msa0353540:CDS	45.0%
!!!	TGGGAGCTGTTTTTTGGACT+TGG	+	chr3_1:23653714-23653733	Msa0353540:CDS	45.0%
!	ACAACGGTGATTTGGGATTG+AGG	-	chr3_1:23652825-23652844	None:intergenic	45.0%
	CGAATGGGTATCTGAGTAGT+GGG	-	chr3_1:23652868-23652887	None:intergenic	45.0%
	TCGAATGGGTATCTGAGTAG+TGG	-	chr3_1:23652869-23652888	None:intergenic	45.0%
!!	TGAACTAGGGTTTGTTGTGG+TGG	-	chr3_1:23652962-23652981	None:intergenic	45.0%
	AAAGGTTTGGGTGGGAGATA+TGG	-	chr3_1:23653043-23653062	None:intergenic	45.0%
	GGCACAGGATTGAAAGGAAA+AGG	-	chr3_1:23653061-23653080	None:intergenic	45.0%
	CAGATTCTCCCTCCTTCATT+TGG	+	chr3_1:23653090-23653109	Msa0353540:CDS	45.0%
!!	GTGACAGCTACGACTTTTTC+AGG	-	chr3_1:23653172-23653191	None:intergenic	45.0%
	CTAAGCGTACCGAGTATGAT+AGG	-	chr3_1:23653211-23653230	None:intergenic	45.0%
!!!	AGAGTGCTGGTTTTGAGGTA+CGG	-	chr3_1:23653240-23653259	None:intergenic	45.0%
	ATGCGGCTGTGATAATTGGA+AGG	+	chr3_1:23653264-23653283	Msa0353540:CDS	45.0%
!	GGAGACATCTGCCTTTTCAA+TGG	+	chr3_1:23653313-23653332	Msa0353540:CDS	45.0%
!	ACATCTGCCTTTTCAATGGG+CGG	+	chr3_1:23653317-23653336	Msa0353540:CDS	45.0%
	GGTAGACAAAACTGGAAGGA+CGG	+	chr3_1:23653348-23653367	Msa0353540:CDS	45.0%
	TGGGTGAAGTTGGCGAATTT+AGG	+	chr3_1:23653580-23653599	Msa0353540:CDS	45.0%
	GGGTGAAGTTGGCGAATTTA+GGG	+	chr3_1:23653581-23653600	Msa0353540:CDS	45.0%
	GGTGAAGTTGGCGAATTTAG+GGG	+	chr3_1:23653582-23653601	Msa0353540:CDS	45.0%
!!!	TGGGAGCTGTTTTTTGGACT+TGG	+	chr3_1:23653714-23653733	Msa0353540:CDS	45.0%
!!	GATTGAGGAAGAGCGCCAAT+TGG	-	chr3_1:23652810-23652829	None:intergenic	50.0%
!!	TCTGAGTAGTGGGTACTCGA+GGG	-	chr3_1:23652858-23652877	None:intergenic	50.0%
!!	ATCTGAGTAGTGGGTACTCG+AGG	-	chr3_1:23652859-23652878	None:intergenic	50.0%
!!	GTTGTGGTGGTGGTTTGATG+AGG	-	chr3_1:23652949-23652968	None:intergenic	50.0%
!!	ACTAGGGTTTGTTGTGGTGG+TGG	-	chr3_1:23652959-23652978	None:intergenic	50.0%
!!	CGGTGAACTAGGGTTTGTTG+TGG	-	chr3_1:23652965-23652984	None:intergenic	50.0%
	AAACCCTAGTTCACCGTCCT+TGG	+	chr3_1:23652969-23652988	Msa0353540:CDS	50.0%
	CAACCAAGGACGGTGAACTA+GGG	-	chr3_1:23652975-23652994	None:intergenic	50.0%
	TCAACCAAGGACGGTGAACT+AGG	-	chr3_1:23652976-23652995	None:intergenic	50.0%
	GATAAGGCCGCCCATTGAAA+AGG	-	chr3_1:23653327-23653346	None:intergenic	50.0%
!	TTTCAATGGGCGGCCTTATC+TGG	+	chr3_1:23653327-23653346	Msa0353540:CDS	50.0%
	ACTGGAAGGACGGTTACTGT+TGG	+	chr3_1:23653358-23653377	Msa0353540:CDS	50.0%
	AAGGACGGTTACTGTTGGAC+CGG	+	chr3_1:23653363-23653382	Msa0353540:CDS	50.0%
	TTCAGCCACTAACTGGACAG+TGG	-	chr3_1:23653395-23653414	None:intergenic	50.0%
	CCAAAGGTTCAGCCACTAAC+TGG	-	chr3_1:23653402-23653421	None:intergenic	50.0%
	CCAGTTAGTGGCTGAACCTT+TGG	+	chr3_1:23653399-23653418	Msa0353540:CDS	50.0%
	GTGGCTGAACCTTTGGTTGA+TGG	+	chr3_1:23653406-23653425	Msa0353540:CDS	50.0%
!!	GCTGAACCTTTGGTTGATGG+TGG	+	chr3_1:23653409-23653428	Msa0353540:CDS	50.0%
!!	GAACCTTTGGTTGATGGTGG+CGG	+	chr3_1:23653412-23653431	Msa0353540:CDS	50.0%
!!	AACCTTTGGTTGATGGTGGC+GGG	+	chr3_1:23653413-23653432	Msa0353540:CDS	50.0%
	GGAGAAGAAGTGGGTGAAGT+TGG	+	chr3_1:23653570-23653589	Msa0353540:CDS	50.0%
!	CTTCTTTTGGCGATCCACTC+TGG	-	chr3_1:23653802-23653821	None:intergenic	50.0%
!!	GATTGAGGAAGAGCGCCAAT+TGG	-	chr3_1:23652810-23652829	None:intergenic	50.0%
!!	TCTGAGTAGTGGGTACTCGA+GGG	-	chr3_1:23652858-23652877	None:intergenic	50.0%
!!	ATCTGAGTAGTGGGTACTCG+AGG	-	chr3_1:23652859-23652878	None:intergenic	50.0%
!!	GTTGTGGTGGTGGTTTGATG+AGG	-	chr3_1:23652949-23652968	None:intergenic	50.0%
!!	ACTAGGGTTTGTTGTGGTGG+TGG	-	chr3_1:23652959-23652978	None:intergenic	50.0%
!!	CGGTGAACTAGGGTTTGTTG+TGG	-	chr3_1:23652965-23652984	None:intergenic	50.0%
	AAACCCTAGTTCACCGTCCT+TGG	+	chr3_1:23652969-23652988	Msa0353540:CDS	50.0%
	CAACCAAGGACGGTGAACTA+GGG	-	chr3_1:23652975-23652994	None:intergenic	50.0%
	TCAACCAAGGACGGTGAACT+AGG	-	chr3_1:23652976-23652995	None:intergenic	50.0%
	GATAAGGCCGCCCATTGAAA+AGG	-	chr3_1:23653327-23653346	None:intergenic	50.0%
!	TTTCAATGGGCGGCCTTATC+TGG	+	chr3_1:23653327-23653346	Msa0353540:CDS	50.0%
	ACTGGAAGGACGGTTACTGT+TGG	+	chr3_1:23653358-23653377	Msa0353540:CDS	50.0%
	AAGGACGGTTACTGTTGGAC+CGG	+	chr3_1:23653363-23653382	Msa0353540:CDS	50.0%
	TTCAGCCACTAACTGGACAG+TGG	-	chr3_1:23653395-23653414	None:intergenic	50.0%
	CCAAAGGTTCAGCCACTAAC+TGG	-	chr3_1:23653402-23653421	None:intergenic	50.0%
	CCAGTTAGTGGCTGAACCTT+TGG	+	chr3_1:23653399-23653418	Msa0353540:CDS	50.0%
	GTGGCTGAACCTTTGGTTGA+TGG	+	chr3_1:23653406-23653425	Msa0353540:CDS	50.0%
!!	GCTGAACCTTTGGTTGATGG+TGG	+	chr3_1:23653409-23653428	Msa0353540:CDS	50.0%
!!	GAACCTTTGGTTGATGGTGG+CGG	+	chr3_1:23653412-23653431	Msa0353540:CDS	50.0%
!!	AACCTTTGGTTGATGGTGGC+GGG	+	chr3_1:23653413-23653432	Msa0353540:CDS	50.0%
	GGAGAAGAAGTGGGTGAAGT+TGG	+	chr3_1:23653570-23653589	Msa0353540:CDS	50.0%
!	CTTCTTTTGGCGATCCACTC+TGG	-	chr3_1:23653802-23653821	None:intergenic	50.0%
!	CAGCCGCATTTCAAGAGTGC+TGG	-	chr3_1:23653253-23653272	None:intergenic	55.0%
	TCCTGACATGTCGCCTTGCT+CGG	+	chr3_1:23653291-23653310	Msa0353540:CDS	55.0%
	AACTGGACAGTGGAGTCGTC+CGG	-	chr3_1:23653385-23653404	None:intergenic	55.0%
	CGACTCCACTGTCCAGTTAG+TGG	+	chr3_1:23653387-23653406	Msa0353540:CDS	55.0%
	TTCCCGCCACCATCAACCAA+AGG	-	chr3_1:23653418-23653437	None:intergenic	55.0%
	TGTTATGGCCACCTCCAGAG+TGG	+	chr3_1:23653785-23653804	Msa0353540:CDS	55.0%
!	CTTTTGGCGATCCACTCTGG+AGG	-	chr3_1:23653799-23653818	None:intergenic	55.0%
!	CAGCCGCATTTCAAGAGTGC+TGG	-	chr3_1:23653253-23653272	None:intergenic	55.0%
	TCCTGACATGTCGCCTTGCT+CGG	+	chr3_1:23653291-23653310	Msa0353540:CDS	55.0%
	AACTGGACAGTGGAGTCGTC+CGG	-	chr3_1:23653385-23653404	None:intergenic	55.0%
	CGACTCCACTGTCCAGTTAG+TGG	+	chr3_1:23653387-23653406	Msa0353540:CDS	55.0%
	TTCCCGCCACCATCAACCAA+AGG	-	chr3_1:23653418-23653437	None:intergenic	55.0%
	TGTTATGGCCACCTCCAGAG+TGG	+	chr3_1:23653785-23653804	Msa0353540:CDS	55.0%
!	CTTTTGGCGATCCACTCTGG+AGG	-	chr3_1:23653799-23653818	None:intergenic	55.0%
	CCTGACATGTCGCCTTGCTC+GGG	+	chr3_1:23653292-23653311	Msa0353540:CDS	60.0%
	AGGCAGATGTCTCCCGAGCA+AGG	-	chr3_1:23653307-23653326	None:intergenic	60.0%
!	TTGGCGATCCACTCTGGAGG+TGG	-	chr3_1:23653796-23653815	None:intergenic	60.0%
	CCTGACATGTCGCCTTGCTC+GGG	+	chr3_1:23653292-23653311	Msa0353540:CDS	60.0%
	AGGCAGATGTCTCCCGAGCA+AGG	-	chr3_1:23653307-23653326	None:intergenic	60.0%
!	TTGGCGATCCACTCTGGAGG+TGG	-	chr3_1:23653796-23653815	None:intergenic	60.0%
	CCCGAGCAAGGCGACATGTC+AGG	-	chr3_1:23653295-23653314	None:intergenic	65.0%
	CCCGAGCAAGGCGACATGTC+AGG	-	chr3_1:23653295-23653314	None:intergenic	65.0%
	TGGCGACGGCGGAGGCAGAC+TGG	+	chr3_1:23652699-23652718	Msa0353540:CDS	75.0%
	TGGCGACGGCGGAGGCAGAC+TGG	+	chr3_1:23652699-23652718	Msa0353540:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	23652698	23653825	23652698	ID=Msa0353540;Name=Msa0353540
chr3_1	mRNA	23652698	23653825	23652698	ID=Msa0353540-mRNA-1;Parent=Msa0353540;Name=Msa0353540-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|375
chr3_1	exon	23652698	23653825	23652698	ID=Msa0353540-mRNA-1:exon:5280;Parent=Msa0353540-mRNA-1
chr3_1	CDS	23652698	23653825	23652698	ID=Msa0353540-mRNA-1:cds;Parent=Msa0353540-mRNA-1

Gene Sequence

>Msa0353540

ATGGCGACGGCGGAGGCAGACTGGTCTCAACTGCCGAAAGATCTTCTTCATCTGATATCACAACGAATGGACAACGAAATCGATCTTATTCGTTTTCGATCAATTTGTTCCAATTGGCGCTCTTCCTCAATCCCAAATCACCGTTGTAACATTTTAACCCTCGAGTACCCACTACTCAGATACCCATTCGAAATTGATTCCCTCAACAACAATACTTCATTCTCTTACCTTTCCAAACACAGTTTCTTCCTCATCAAACCACCACCACAACAAACCCTAGTTCACCGTCCTTGGTTGATCAGAATTACCCAAAATTCTAGTGGCAAAACCCAACTCTTACATCCATATCTCCCACCCAAACCTTTTCCTTTCAATCCTGTGCCCAATTTTAACAGATTCTCCCTCCTTCATTTGGTAACTAACTTCATCATTCCCGACAAAAAAGAACCCTTGCACTATACCGAAATGTACCCTGAAAAAGTCGTAGCTGTCACATGTCATGACAAAAAACCTATCATACTCGGTACGCTTAGCTTTAGCCGTACCTCAAAACCAGCACTCTTGAAATGCGGCTGTGATAATTGGAAGGCTATTCCTGACATGTCGCCTTGCTCGGGAGACATCTGCCTTTTCAATGGGCGGCCTTATCTGGTAGACAAAACTGGAAGGACGGTTACTGTTGGACCGGACGACTCCACTGTCCAGTTAGTGGCTGAACCTTTGGTTGATGGTGGCGGGAAAAGAAAATTCTTGGTGGAGAGTGAGGGTGAGTTATTGCTAACGGACTTTTATGAGTCTTTAGAGTTCGATGGTGATGATCTTGCAAGTATTAATTTCTTGGTACGGATTCATGTGTTTAAGCTTAATGAAAAGGAGAAGAAGTGGGTGAAGTTGGCGAATTTAGGGGATAGAGTTTTGTTTTTGGGATCGCTGTGTTCATTCTCTGCTTCTGCTTCAGATTTGTGTGTTGCCAAAGGGAATTGTGTGATCATTATGGATAATATATCCACTTTATGTGGGAGCTGTTTTTTGGACTTGGATGATGGTCAGCTTTCGCTTTTATCCAAATATCCTGAATATTCTAAATTGTTATGGCCACCTCCAGAGTGGATCGCCAAAAGAAGCTGA

Protein sequence

>Msa0353540

MATAEADWSQLPKDLLHLISQRMDNEIDLIRFRSICSNWRSSSIPNHRCNILTLEYPLLRYPFEIDSLNNNTSFSYLSKHSFFLIKPPPQQTLVHRPWLIRITQNSSGKTQLLHPYLPPKPFPFNPVPNFNRFSLLHLVTNFIIPDKKEPLHYTEMYPEKVVAVTCHDKKPIILGTLSFSRTSKPALLKCGCDNWKAIPDMSPCSGDICLFNGRPYLVDKTGRTVTVGPDDSTVQLVAEPLVDGGGKRKFLVESEGELLLTDFYESLEFDGDDLASINFLVRIHVFKLNEKEKKWVKLANLGDRVLFLGSLCSFSASASDLCVAKGNCVIIMDNISTLCGSCFLDLDDGQLSLLSKYPEYSKLLWPPPEWIAKRS*