AlfalfaGEDB | Msa0428010

M. sativa cultivar zhongmu-4 / Msa0428010

KEGG: -
GO: GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015172 GO:0015175 GO:0015318 GO:0015399 GO:0015711 GO:0015800 GO:0015804 GO:0015849 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039
PFAM: PF01490.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0428010	XP_003602666.1	95.556	90	4	0	1	90	388	477	9.14e-51	176

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0428010	sp\|Q8GUM3\|AAP5_ARATH	60.000	90	36	0	1	90	391	480	4.81e-36	129

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0428010	G7JC68	95.556	90	4	0	1	90	388	477	4.36e-51	176

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0142220	Msa0428010	0.802760	4.815158e-49	-8.615850e-47
Msa0232850	Msa0428010	0.800161	1.640807e-48	-8.615850e-47
Msa0378360	Msa0428010	0.848573	5.621933e-60	-8.615850e-47
Msa0384890	Msa0428010	0.812316	4.506821e-51	-8.615850e-47
Msa0427710	Msa0428010	0.844953	5.493967e-59	-8.615850e-47
Msa0428000	Msa0428010	0.902146	1.306304e-78	-8.615850e-47
Msa0428010	Msa0436780	0.805186	1.507567e-49	-8.615850e-47
Msa0428010	Msa0452900	0.813727	2.211152e-51	-8.615850e-47
Msa0428010	Msa0453120	0.817700	2.878333e-52	-8.615850e-47
Msa0428010	Msa0479340	0.807721	4.401770e-50	-8.615850e-47
Msa0428010	Msa0518540	0.855983	4.372114e-62	-8.615850e-47
Msa0428010	Msa0729800	0.804314	2.292738e-49	-8.615850e-47
Msa0428010	Msa0743810	0.806151	9.454551e-50	-8.615850e-47
Msa0428010	Msa0783160	0.816654	4.948194e-52	-8.615850e-47
Msa0428010	Msa0808010	0.816181	6.312240e-52	-8.615850e-47
Msa0428010	Msa0808020	0.810587	1.070313e-50	-8.615850e-47
Msa0428010	Msa0858980	0.817917	2.571752e-52	-8.615850e-47
Msa0428010	Msa0981370	0.807877	4.078526e-50	-8.615850e-47
Msa0428010	Msa1044840	0.801339	9.434312e-49	-8.615850e-47
Msa0428010	Msa1092990	0.815190	1.049744e-51	-8.615850e-47
Msa0428010	Msa1100980	0.825531	4.454667e-54	-8.615850e-47
Msa0428010	Msa1121200	0.828876	7.045134e-55	-8.615850e-47
Msa0428010	Msa1135740	0.813932	1.993050e-51	-8.615850e-47
Msa0428010	Msa1187950	0.815866	7.422600e-52	-8.615850e-47
Msa0428010	Msa1270230	0.805648	1.206064e-49	-8.615850e-47
Msa0428010	Msa1283330	0.800839	1.194019e-48	-8.615850e-47
Msa0428010	Msa1285930	0.811448	6.965562e-51	-8.615850e-47
Msa0428010	Msa1382690	0.834807	2.420902e-56	-8.615850e-47
Msa0428010	Msa1382700	0.868964	4.413698e-66	-8.615850e-47
Msa0428010	Msa1433120	0.819110	1.379241e-52	-8.615850e-47
Msa0360510	Msa0428010	0.833271	5.869777e-56	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0428010	MtrunA17_Chr3g0130641	95.556	90	4	0	1	90	388	477	8.40e-55	176
Msa0428010	MtrunA17_Chr3g0125081	66.667	90	30	0	1	90	390	479	4.83e-40	137
Msa0428010	MtrunA17_Chr3g0130661	68.966	87	27	0	1	87	92	178	9.46e-40	129
Msa0428010	MtrunA17_Chr3g0125101	65.556	90	31	0	1	90	391	480	7.67e-39	134
Msa0428010	MtrunA17_Chr3g0125091	64.444	90	32	0	1	90	391	480	1.52e-37	130
Msa0428010	MtrunA17_Chr8g0384661	62.500	88	33	0	3	90	407	494	2.00e-36	127
Msa0428010	MtrunA17_Chr4g0037201	58.889	90	37	0	1	90	423	512	1.55e-35	125
Msa0428010	MtrunA17_Chr1g0159761	58.889	90	37	0	1	90	386	475	1.34e-30	112
Msa0428010	MtrunA17_Chr1g0206821	56.180	89	39	0	1	89	442	530	1.08e-29	109
Msa0428010	MtrunA17_Chr1g0206851	52.874	87	41	0	1	87	292	378	1.77e-29	107
Msa0428010	MtrunA17_Chr3g0130651	73.563	87	23	0	1	87	379	465	1.89e-28	105
Msa0428010	MtrunA17_Chr3g0140761	47.191	89	47	0	1	89	393	481	9.84e-27	101
Msa0428010	MtrunA17_Chr1g0206871	49.438	89	45	0	1	89	382	470	1.74e-26	100
Msa0428010	MtrunA17_Chr1g0146491	48.315	89	46	0	1	89	383	471	7.69e-26	99.0
Msa0428010	MtrunA17_Chr1g0206831	54.321	81	37	0	1	81	255	335	9.49e-26	97.4
Msa0428010	MtrunA17_Chr5g0447221	54.430	79	36	0	1	79	385	463	2.53e-25	97.4
Msa0428010	MtrunA17_Chr3g0140531	47.674	86	45	0	1	86	382	467	1.30e-24	95.5
Msa0428010	MtrunA17_Chr3g0103251	51.899	79	38	0	1	79	292	370	6.39e-24	92.8
Msa0428010	MtrunA17_Chr3g0103231	51.899	79	38	0	1	79	379	457	7.79e-24	93.2
Msa0428010	MtrunA17_Chr3g0140541	47.500	80	42	0	7	86	387	466	6.51e-23	90.9
Msa0428010	MtrunA17_Chr3g0103211	48.101	79	41	0	1	79	379	457	1.31e-22	89.7
Msa0428010	MtrunA17_Chr3g0103221	49.367	79	40	0	1	79	386	464	1.38e-22	89.7
Msa0428010	MtrunA17_Chr1g0146391	45.977	87	47	0	1	87	368	454	1.94e-21	86.7
Msa0428010	MtrunA17_Chr1g0146331	42.529	87	50	0	1	87	377	463	3.32e-21	85.9
Msa0428010	MtrunA17_Chr2g0293331	41.379	87	51	0	1	87	368	454	7.28e-20	82.0
Msa0428010	MtrunA17_Chr1g0146411	39.286	84	51	0	3	86	386	469	2.96e-19	80.5
Msa0428010	MtrunA17_Chr3g0103271	46.667	75	40	0	4	78	387	461	1.01e-18	79.0
Msa0428010	MtrunA17_Chr5g0447231	34.211	76	50	0	4	79	385	460	1.55e-15	70.1
Msa0428010	MtrunA17_Chr1g0184721	34.444	90	55	1	1	86	348	437	8.59e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0428010	AT1G44100.1	60.000	90	36	1	90	391	480	4.89e-37	129
Msa0428010	AT5G09220.1	61.111	90	35	1	90	404	493	9.26e-37	129
Msa0428010	AT5G63850.1	57.778	90	38	1	90	377	466	6.12e-35	124
Msa0428010	AT1G77380.1	62.222	90	34	1	90	387	476	4.27e-33	119
Msa0428010	AT5G49630.1	56.818	88	38	1	88	393	480	7.26e-30	110
Msa0428010	AT1G58360.1	53.333	90	42	1	90	395	484	1.95e-29	109
Msa0428010	AT1G10010.1	53.409	88	41	1	88	386	473	5.33e-29	107
Msa0428010	AT1G10010.2	53.409	88	41	1	88	387	474	5.42e-29	107
Msa0428010	AT5G23810.1	55.172	58	26	1	58	386	443	8.88e-17	73.9

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCATTTAGAACTCCATTT+TGG	0.108522	3_2:-78735091	None:intergenic
TCCTTTCTTTAATGATGTTC+TGG	0.137749	3_2:+78734991	Msa0428010:CDS
TTCTTGGAGCAGTGGGATTT+TGG	0.180772	3_2:+78735017	Msa0428010:CDS
AATGATGTTCTGGGATTTCT+TGG	0.212555	3_2:+78735001	Msa0428010:CDS
ACTGGATAATAAACTGTTAA+AGG	0.224075	3_2:-78735040	None:intergenic
CCTTTCTTTAATGATGTTCT+GGG	0.306108	3_2:+78734992	Msa0428010:CDS
TCCCAAAATGGAGTTCTAAA+TGG	0.320619	3_2:+78735089	Msa0428010:CDS
TGAATGGCTTGTAATGCTTT+AGG	0.330057	3_2:-78735195	None:intergenic
GTATCAGTTGTGGCTGCTCT+TGG	0.389248	3_2:+78735148	Msa0428010:CDS
CTTTAGGTCCTCTATAATAC+TGG	0.396377	3_2:-78735179	None:intergenic
CTGGGATTTCTTGGAGCAGT+GGG	0.412618	3_2:+78735010	Msa0428010:CDS
TGCAAAGGAAGATCCCAAAA+TGG	0.417168	3_2:+78735077	Msa0428010:CDS
ACATTGCAACAAAAGTAGTT+AGG	0.463138	3_2:-78734964	None:intergenic
CCCAGAACATCATTAAAGAA+AGG	0.503059	3_2:-78734992	None:intergenic
CTCAACCGCCAGTATTATAG+AGG	0.511484	3_2:+78735171	Msa0428010:CDS
ATGCTTTATTGTATCAGTTG+TGG	0.512651	3_2:+78735138	Msa0428010:CDS
GCTCAATACTCTGAGCTGAA+TGG	0.555896	3_2:-78735211	None:intergenic
TCTGGGATTTCTTGGAGCAG+TGG	0.566045	3_2:+78735009	Msa0428010:CDS
TTTAACAGTTTATTATCCAG+TGG	0.568524	3_2:+78735042	Msa0428010:CDS
TGCAAGATATACATCTCCAC+TGG	0.575990	3_2:-78735058	None:intergenic
TGGAGATGTATATCTTGCAA+AGG	0.629124	3_2:+78735062	Msa0428010:CDS
TAGGTCCTCTATAATACTGG+CGG	0.635831	3_2:-78735176	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTGGATAATAAACTGTTAA+AGG	-	chr3_2:78735043-78735062	None:intergenic	25.0%
!	TTTAACAGTTTATTATCCAG+TGG	+	chr3_2:78735042-78735061	Msa0428010:CDS	25.0%
	ACATTGCAACAAAAGTAGTT+AGG	-	chr3_2:78734967-78734986	None:intergenic	30.0%
	TCCTTTCTTTAATGATGTTC+TGG	+	chr3_2:78734991-78735010	Msa0428010:CDS	30.0%
	CCTTTCTTTAATGATGTTCT+GGG	+	chr3_2:78734992-78735011	Msa0428010:CDS	30.0%
!!	TCCATTTAGAACTCCATTTT+GGG	-	chr3_2:78735093-78735112	None:intergenic	30.0%
!!	ATCCATTTAGAACTCCATTT+TGG	-	chr3_2:78735094-78735113	None:intergenic	30.0%
!!!	GAGTTCTAAATGGATTTTGT+TGG	+	chr3_2:78735099-78735118	Msa0428010:CDS	30.0%
!	ATGCTTTATTGTATCAGTTG+TGG	+	chr3_2:78735138-78735157	Msa0428010:CDS	30.0%
	CCCAGAACATCATTAAAGAA+AGG	-	chr3_2:78734995-78735014	None:intergenic	35.0%
!!	AATGATGTTCTGGGATTTCT+TGG	+	chr3_2:78735001-78735020	Msa0428010:CDS	35.0%
	TGGAGATGTATATCTTGCAA+AGG	+	chr3_2:78735062-78735081	Msa0428010:CDS	35.0%
!!	TCCCAAAATGGAGTTCTAAA+TGG	+	chr3_2:78735089-78735108	Msa0428010:CDS	35.0%
	CTTTAGGTCCTCTATAATAC+TGG	-	chr3_2:78735182-78735201	None:intergenic	35.0%
!	TGAATGGCTTGTAATGCTTT+AGG	-	chr3_2:78735198-78735217	None:intergenic	35.0%
	TGCAAGATATACATCTCCAC+TGG	-	chr3_2:78735061-78735080	None:intergenic	40.0%
	TGCAAAGGAAGATCCCAAAA+TGG	+	chr3_2:78735077-78735096	Msa0428010:CDS	40.0%
	TAGGTCCTCTATAATACTGG+CGG	-	chr3_2:78735179-78735198	None:intergenic	40.0%
!	TTCTTGGAGCAGTGGGATTT+TGG	+	chr3_2:78735017-78735036	Msa0428010:CDS	45.0%
	CTCAACCGCCAGTATTATAG+AGG	+	chr3_2:78735171-78735190	Msa0428010:CDS	45.0%
!	TCTGGGATTTCTTGGAGCAG+TGG	+	chr3_2:78735009-78735028	Msa0428010:CDS	50.0%
	CTGGGATTTCTTGGAGCAGT+GGG	+	chr3_2:78735010-78735029	Msa0428010:CDS	50.0%
	GTATCAGTTGTGGCTGCTCT+TGG	+	chr3_2:78735148-78735167	Msa0428010:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	78734959	78735231	78734959	ID=Msa0428010;Name=Msa0428010
chr3_2	mRNA	78734959	78735231	78734959	ID=Msa0428010-mRNA-1;Parent=Msa0428010;Name=Msa0428010-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|90
chr3_2	exon	78734959	78735231	78734959	ID=Msa0428010-mRNA-1:exon:20964;Parent=Msa0428010-mRNA-1
chr3_2	CDS	78734959	78735231	78734959	ID=Msa0428010-mRNA-1:cds;Parent=Msa0428010-mRNA-1

Gene Sequence	>Msa0428010 ATGATCCTAACTACTTTTGTTGCAATGTTGATTCCTTTCTTTAATGATGTTCTGGGATTTCTTGGAGCAGTGGGATTTTGGCCTTTAACAGTTTATTATCCAGTGGAGATGTATATCTTGCAAAGGAAGATCCCAAAATGGAGTTCTAAATGGATTTTGTTGGAAATCATAAGTGTTATATGCTTTATTGTATCAGTTGTGGCTGCTCTTGGCTCAACCGCCAGTATTATAGAGGACCTAAAGCATTACAAGCCATTCAGCTCAGAGTATTGA
Protein sequence	>Msa0428010 MILTTFVAMLIPFFNDVLGFLGAVGFWPLTVYYPVEMYILQRKIPKWSSKWILLEIISVICFIVSVVAALGSTASIIEDLKHYKPFSSEY*