AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0518540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518540 MtrunA17_Chr3g0130641 95.597 477 21 0 1 477 1 477 0.0 947
Msa0518540 MtrunA17_Chr3g0130651 71.767 464 129 1 11 474 4 465 0.0 674
Msa0518540 MtrunA17_Chr3g0125081 63.410 481 170 3 1 477 1 479 0.0 652
Msa0518540 MtrunA17_Chr8g0384661 67.627 451 146 0 27 477 44 494 0.0 652
Msa0518540 MtrunA17_Chr3g0125091 63.693 482 168 4 1 477 1 480 0.0 643
Msa0518540 MtrunA17_Chr3g0125101 62.241 482 175 4 1 477 1 480 0.0 632
Msa0518540 MtrunA17_Chr4g0037201 59.794 485 183 3 5 477 28 512 0.0 619
Msa0518540 MtrunA17_Chr1g0159761 63.113 469 165 3 13 477 11 475 0.0 607
Msa0518540 MtrunA17_Chr1g0146491 52.790 466 213 4 18 476 6 471 0.0 533
Msa0518540 MtrunA17_Chr3g0140761 51.452 482 227 5 1 476 1 481 0.0 530
Msa0518540 MtrunA17_Chr1g0206821 47.510 522 209 5 20 476 9 530 5.42e-170 489
Msa0518540 MtrunA17_Chr1g0206871 50.109 457 214 4 33 476 15 470 1.88e-168 483
Msa0518540 MtrunA17_Chr1g0146411 47.589 477 240 4 1 474 1 470 2.17e-164 473
Msa0518540 MtrunA17_Chr3g0103231 49.324 444 220 2 26 466 16 457 3.04e-164 472
Msa0518540 MtrunA17_Chr3g0103211 49.550 444 219 2 26 466 16 457 1.35e-160 462
Msa0518540 MtrunA17_Chr3g0140531 47.598 458 227 5 24 473 15 467 7.00e-156 451
Msa0518540 MtrunA17_Chr3g0103221 47.500 440 226 2 30 466 27 464 8.92e-155 448
Msa0518540 MtrunA17_Chr5g0447221 45.842 469 244 5 2 466 1 463 2.28e-153 444
Msa0518540 MtrunA17_Chr3g0103271 48.043 460 233 3 10 465 4 461 3.82e-149 433
Msa0518540 MtrunA17_Chr3g0140541 48.690 458 228 4 18 473 14 466 2.38e-148 432
Msa0518540 MtrunA17_Chr1g0146331 44.592 453 243 4 27 474 14 463 2.44e-147 429
Msa0518540 MtrunA17_Chr1g0146391 46.374 455 231 5 27 474 6 454 2.82e-147 428
Msa0518540 MtrunA17_Chr2g0293331 45.197 458 243 5 21 474 1 454 4.19e-146 425
Msa0518540 MtrunA17_Chr5g0447231 43.836 438 240 4 33 466 25 460 2.32e-127 378
Msa0518540 MtrunA17_Chr1g0206851 48.634 366 177 4 114 474 19 378 1.26e-126 373
Msa0518540 MtrunA17_Chr5g0402151 60.339 295 84 3 36 330 3 264 1.60e-115 341
Msa0518540 MtrunA17_Chr1g0206831 49.107 336 160 3 138 468 6 335 4.04e-112 334
Msa0518540 MtrunA17_Chr3g0103251 45.739 352 157 3 147 466 21 370 2.87e-109 328
Msa0518540 MtrunA17_Chr3g0130671 64.935 231 81 0 16 246 22 252 8.92e-108 321
Msa0518540 MtrunA17_Chr3g0130661 66.854 178 59 0 297 474 1 178 1.80e-83 255
Msa0518540 MtrunA17_Chr6g0458931 29.801 453 268 10 33 474 42 455 5.36e-49 174
Msa0518540 MtrunA17_Chr2g0331721 29.464 448 263 11 4 438 18 425 5.80e-48 171
Msa0518540 MtrunA17_Chr5g0422741 32.334 467 254 16 20 470 15 435 8.25e-47 167
Msa0518540 MtrunA17_Chr8g0376991 28.728 456 282 12 28 474 15 436 2.05e-45 164
Msa0518540 MtrunA17_Chr1g0184721 27.313 454 291 13 27 473 16 437 1.15e-44 162
Msa0518540 MtrunA17_Chr4g0057851 28.728 456 261 14 34 471 11 420 1.73e-43 158
Msa0518540 MtrunA17_Chr1g0198531 30.238 463 286 11 13 470 3 433 8.83e-43 157
Msa0518540 MtrunA17_Chr6g0459031 30.217 460 279 12 24 474 2 428 5.10e-40 149
Msa0518540 MtrunA17_Chr7g0230011 28.664 464 294 10 17 474 11 443 9.95e-40 149
Msa0518540 MtrunA17_Chr6g0458921 29.268 451 272 10 34 474 15 428 1.18e-39 148
Msa0518540 MtrunA17_Chr1g0198541 28.665 457 268 10 29 470 20 433 5.13e-39 146
Msa0518540 MtrunA17_Chr4g0027241 27.004 474 284 14 27 475 25 461 1.81e-37 142
Msa0518540 MtrunA17_Chr3g0103261 49.600 125 63 0 26 150 16 140 4.50e-37 134
Msa0518540 MtrunA17_Chr3g0077651 29.754 447 278 10 34 474 53 469 1.53e-36 140
Msa0518540 MtrunA17_Chr6g0459021 27.615 478 304 13 5 474 4 447 4.69e-36 138
Msa0518540 MtrunA17_Chr1g0206841 62.105 95 36 0 20 114 8 102 9.36e-36 128
Msa0518540 MtrunA17_Chr2g0328081 28.696 460 286 10 24 474 2 428 1.87e-35 136
Msa0518540 MtrunA17_Chr3g0122921 26.471 476 293 14 4 474 5 428 3.34e-35 135
Msa0518540 MtrunA17_Chr6g0459011 29.343 426 261 11 49 468 1 392 1.64e-34 133
Msa0518540 MtrunA17_Chr8g0387941 26.501 483 304 17 7 476 6 450 6.12e-34 132
Msa0518540 MtrunA17_Chr1g0206861 52.500 120 56 1 20 138 8 127 1.19e-33 124
Msa0518540 MtrunA17_Chr6g0459001 27.383 493 295 12 27 474 2 476 1.04e-32 129
Msa0518540 MtrunA17_Chr1g0198081 26.966 445 280 16 44 476 70 481 1.49e-32 129
Msa0518540 MtrunA17_Chr1g0146351 54.369 103 46 1 43 145 13 114 1.95e-30 114
Msa0518540 MtrunA17_Chr4g0038711 25.104 482 316 16 11 472 45 501 2.27e-29 120
Msa0518540 MtrunA17_Chr3g0143701 25.216 464 282 14 27 464 75 499 1.54e-28 118
Msa0518540 MtrunA17_Chr5g0406351 23.810 462 306 12 19 465 93 523 2.69e-23 102
Msa0518540 MtrunA17_Chr5g0436281 25.962 416 267 14 64 475 4 382 6.40e-23 100
Msa0518540 MtrunA17_Chr5g0406331 23.341 437 286 12 20 440 73 476 7.23e-21 95.5
Msa0518540 MtrunA17_Chr2g0281341 24.845 483 290 18 19 472 81 519 4.24e-18 87.0
Msa0518540 MtrunA17_Chr4g0058161 22.353 425 271 12 64 475 71 449 6.89e-16 79.7
Msa0518540 MtrunA17_Chr1g0159751 42.683 82 40 1 276 357 1 75 2.46e-15 70.9
Msa0518540 MtrunA17_Chr3g0112971 24.059 478 290 18 22 475 7 435 1.02e-14 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518540 AT5G09220.1 64.819 469 161 2 13 477 25 493 0.0 656
Msa0518540 AT1G77380.1 65.517 464 157 1 17 477 13 476 0.0 649
Msa0518540 AT5G63850.1 64.912 456 156 2 26 477 11 466 0.0 642
Msa0518540 AT1G44100.1 59.158 475 184 4 12 477 7 480 0.0 610
Msa0518540 AT5G49630.1 56.798 456 191 4 26 475 25 480 0.0 528
Msa0518540 AT1G10010.1 53.642 453 206 2 27 475 21 473 0.0 526
Msa0518540 AT1G10010.2 53.642 453 206 2 27 475 22 474 0.0 526
Msa0518540 AT1G58360.1 53.145 461 211 3 22 477 24 484 0.0 524
Msa0518540 AT5G23810.1 47.748 444 216 6 7 442 5 440 8.23e-137 402
Msa0518540 AT5G23810.2 48.538 342 165 5 7 345 5 338 4.37e-101 307
Msa0518540 AT5G23810.4 46.586 249 124 4 18 263 14 256 1.54e-67 218
Msa0518540 AT5G23810.5 45.972 211 105 4 7 214 5 209 1.47e-53 180
Msa0518540 AT5G23810.3 45.972 211 105 4 7 214 5 209 1.47e-53 180
Msa0518540 AT1G48640.1 29.325 474 294 12 7 474 15 453 2.74e-47 170
Msa0518540 AT5G40780.1 29.663 445 277 10 34 474 34 446 2.60e-46 167
Msa0518540 AT1G67640.1 29.736 454 280 11 28 474 20 441 1.33e-42 157
Msa0518540 AT5G40780.3 29.535 430 267 10 49 474 1 398 1.95e-42 155
Msa0518540 AT5G40780.2 29.535 430 267 10 49 474 1 398 1.95e-42 155
Msa0518540 AT1G61270.1 27.193 456 293 12 22 469 19 443 7.11e-41 152
Msa0518540 AT1G24400.1 28.510 463 274 15 28 474 20 441 9.89e-41 152
Msa0518540 AT3G01760.1 25.214 468 306 11 16 468 3 441 8.92e-40 149
Msa0518540 AT1G25530.1 28.477 453 270 11 32 471 26 437 6.93e-37 141
Msa0518540 AT1G71680.1 27.423 485 291 14 7 474 8 448 9.68e-35 135
Msa0518540 AT1G08230.2 29.231 455 279 14 33 475 27 450 1.63e-32 129
Msa0518540 AT1G71680.2 28.532 361 211 12 7 350 8 338 3.04e-30 121
Msa0518540 AT5G41800.1 25.219 456 297 12 33 476 26 449 4.72e-26 110
Msa0518540 AT1G08230.4 30.745 322 193 9 163 475 31 331 2.63e-24 103
Msa0518540 AT1G08230.6 30.745 322 193 9 163 475 31 331 2.63e-24 103
Msa0518540 AT1G08230.5 30.745 322 193 9 163 475 31 331 2.63e-24 103
Msa0518540 AT1G08230.3 30.745 322 193 9 163 475 50 350 3.18e-24 103
Msa0518540 AT2G39890.2 24.519 416 273 14 64 475 63 441 2.44e-21 96.7
Msa0518540 AT2G39890.1 24.519 416 273 14 64 475 63 441 2.44e-21 96.7
Msa0518540 AT2G39890.3 24.519 416 273 14 64 475 4 382 3.17e-21 95.9
Msa0518540 AT2G36590.1 24.632 475 309 17 9 475 2 435 5.83e-21 95.5
Msa0518540 AT3G55740.1 24.940 417 270 13 64 475 60 438 1.03e-20 94.7
Msa0518540 AT3G55740.2 25.120 418 268 14 64 475 4 382 1.27e-20 94.0
Msa0518540 AT3G55740.3 25.120 418 268 14 64 475 4 382 1.27e-20 94.0
Msa0518540 AT1G47670.1 24.895 474 301 15 19 472 79 517 2.93e-18 87.8
Msa0518540 AT4G35180.1 23.529 459 292 13 34 473 58 476 2.59e-16 81.6
Msa0518540 AT4G35180.2 25.271 277 187 5 33 305 57 317 2.73e-13 71.2
Msa0518540 AT2G36590.2 24.332 337 224 12 9 340 2 312 9.82e-12 66.6

Find 113 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCATTTAGAACTCCATTT+TGG 0.108522 3_4:+70333222 None:intergenic
GTTCAGTACTTGTATCTTTA+TGG 0.129160 3_4:-70335082 Msa0518540:CDS
TCCTTTCTTTAATGATGTTC+TGG 0.137749 3_4:-70333322 Msa0518540:CDS
TTCTTGGAGCAGTGGGATTT+TGG 0.180772 3_4:-70333296 Msa0518540:CDS
AATGATGTTCTGGGATTTCT+TGG 0.212555 3_4:-70333312 Msa0518540:CDS
ACTGGATAATAAACTGTTAA+AGG 0.224075 3_4:+70333273 None:intergenic
TCAAATTCTCATTGAAATTC+AGG 0.231508 3_4:-70333847 Msa0518540:intron
CCATCGTCGTCGAAGTATTC+AGG 0.273974 3_4:+70335951 None:intergenic
TAAATCTATTTAGATTAGTT+TGG 0.281769 3_4:-70333389 Msa0518540:CDS
CCTTTCTTTAATGATGTTCT+GGG 0.306108 3_4:-70333321 Msa0518540:CDS
TCCCAAAATGGAGTTCTAAA+TGG 0.320619 3_4:-70333224 Msa0518540:CDS
TGAATGGCTTGTAATGCTTT+AGG 0.330057 3_4:+70333118 None:intergenic
TCCAGCTTGGCTCATCGATT+TGG 0.332874 3_4:-70333562 Msa0518540:CDS
TATCTTTATGGCAGTGCAAT+AGG 0.337033 3_4:-70335070 Msa0518540:CDS
ATAGCTGCAATAATGTCTTT+TGG 0.346313 3_4:-70334334 Msa0518540:CDS
AGCATGGGCTACGGCACAAT+TGG 0.349061 3_4:-70335570 Msa0518540:CDS
TCTAGATGTCTCCATTTATC+AGG 0.357700 3_4:-70334469 Msa0518540:CDS
AACGACGATAACCTGCTGTT+TGG 0.358251 3_4:+70334278 None:intergenic
AACCCATACATGATCAGTTT+TGG 0.359942 3_4:-70334418 Msa0518540:CDS
ATCAACTCACATCATGCTAA+TGG 0.372993 3_4:+70335030 None:intergenic
TTCTGAAGGGACACCATCTA+TGG 0.373125 3_4:+70335980 None:intergenic
TAATGTGTACCTGTTCGTTT+AGG 0.376426 3_4:+70335924 None:intergenic
TGCATGGGCTATGCTGCATT+TGG 0.377828 3_4:-70333630 Msa0518540:CDS
CTAAATAGATTTAGATTGTA+AGG 0.384304 3_4:+70333399 None:intergenic
GATGCAGTTCGTAGCATTCT+TGG 0.385053 3_4:-70335422 Msa0518540:intron
CATATAATAACAGCTGTGAT+TGG 0.385307 3_4:-70335611 Msa0518540:CDS
GTATCAGTTGTGGCTGCTCT+TGG 0.389248 3_4:-70333165 Msa0518540:CDS
CTTTAGGTCCTCTATAATAC+TGG 0.396377 3_4:+70333134 None:intergenic
GGGTTGGATTGGTGGTCCTT+TGG 0.399149 3_4:-70335549 Msa0518540:CDS
ATAACAGCTGTGATTGGGTC+TGG 0.401374 3_4:-70335605 Msa0518540:CDS
GTTATGAGTCAGGAACAATA+TGG 0.405471 3_4:-70335646 Msa0518540:intron
ACTCCAAAACTGATCATGTA+TGG 0.410066 3_4:+70334415 None:intergenic
CTGGGATTTCTTGGAGCAGT+GGG 0.412618 3_4:-70333303 Msa0518540:CDS
TGCAAAGGAAGATCCCAAAA+TGG 0.417168 3_4:-70333236 Msa0518540:CDS
ACGACGATAACCTGCTGTTT+GGG 0.418197 3_4:+70334279 None:intergenic
ACAAAGTGACGAGGCTGAAA+AGG 0.428865 3_4:+70335519 None:intergenic
TTATTTGCTTTGTGGCTGCA+TGG 0.440989 3_4:-70333646 Msa0518540:CDS
ACCTTCAAGGGTACTCTCAC+TGG 0.442148 3_4:-70333957 Msa0518540:CDS
AACAGAAAATGGTACCTTCA+AGG 0.444400 3_4:-70333970 Msa0518540:intron
AACCGGAGCCATTCCATAGA+TGG 0.448209 3_4:-70335993 Msa0518540:CDS
CTGGGGTGCTATCCTTAGCA+TGG 0.450709 3_4:-70335586 Msa0518540:CDS
GTCTTTGCCTTCGTAGAGAA+AGG 0.451734 3_4:-70333483 Msa0518540:CDS
TCAACTCACATCATGCTAAT+GGG 0.455025 3_4:+70335031 None:intergenic
AAGAAGAAATAGAAAGAAAC+CGG 0.458125 3_4:-70336010 Msa0518540:CDS
ACATTGCAACAAAAGTAGTT+AGG 0.463138 3_4:+70333349 None:intergenic
CTATTCCACAAACCGTGTAT+TGG 0.465036 3_4:+70335104 None:intergenic
GCATGGGCTACGGCACAATT+GGG 0.465627 3_4:-70335569 Msa0518540:CDS
TCCAGTGAGAGTACCCTTGA+AGG 0.465923 3_4:+70333956 None:intergenic
GCACAATTGGGTTGGATTGG+TGG 0.466018 3_4:-70335557 Msa0518540:CDS
TAAGGACGAAAACCCGGGAT+TGG 0.471537 3_4:+70333417 None:intergenic
GTCAACAACAACAAAATTGT+GGG 0.475137 3_4:-70333916 Msa0518540:CDS
GCCAAATCGATGAGCCAAGC+TGG 0.477067 3_4:+70333561 None:intergenic
GGGCGCCAATACACGGTTTG+TGG 0.479067 3_4:-70335109 Msa0518540:CDS
TATTTGCTTTGTGGCTGCAT+GGG 0.481138 3_4:-70333645 Msa0518540:CDS
TAGCTTCTCTTTCCATGCAC+AGG 0.488301 3_4:+70335455 None:intergenic
ATATAATAACAGCTGTGATT+GGG 0.488499 3_4:-70335610 Msa0518540:CDS
GGGCTACGGCACAATTGGGT+TGG 0.489975 3_4:-70335565 Msa0518540:CDS
TGCTGCTCCTTTCTCTACGA+AGG 0.498588 3_4:+70333476 None:intergenic
GCCGTCGTAATCCACCTTGT+GGG 0.501113 3_4:-70333531 Msa0518540:CDS
CCCAGAACATCATTAAAGAA+AGG 0.503059 3_4:+70333321 None:intergenic
GCACCAGGGAACTTGCTCAC+TGG 0.503377 3_4:-70333600 Msa0518540:CDS
TTCTCTACGAAGGCAAAGAC+AGG 0.507161 3_4:+70333486 None:intergenic
CTCAACCGCCAGTATTATAG+AGG 0.511484 3_4:-70333142 Msa0518540:CDS
ATGCTTTATTGTATCAGTTG+TGG 0.512651 3_4:-70333175 Msa0518540:CDS
GATGGTCGTCCTAAACGAAC+AGG 0.514219 3_4:-70335933 Msa0518540:intron
TTAGCAATTTCCCAAACAGC+AGG 0.515563 3_4:-70334289 Msa0518540:intron
TAACAGCTGTGATTGGGTCT+GGG 0.516920 3_4:-70335604 Msa0518540:CDS
ACAGAAAATGGTACCTTCAA+GGG 0.518566 3_4:-70333969 Msa0518540:intron
TGTCAACAACAACAAAATTG+TGG 0.522475 3_4:-70333917 Msa0518540:CDS
GATTGTAAGGACGAAAACCC+GGG 0.523575 3_4:+70333412 None:intergenic
GGAACACAAAATTCCAATCC+CGG 0.528501 3_4:-70333430 Msa0518540:CDS
GCATTTGGAGAACAAGCACC+AGG 0.529176 3_4:-70333615 Msa0518540:CDS
CACCATCTATGGAATGGCTC+CGG 0.534833 3_4:+70335991 None:intergenic
TGCCGTCGTAATCCACCTTG+TGG 0.547983 3_4:-70333532 Msa0518540:CDS
ACGGCACAATTGGGTTGGAT+TGG 0.551307 3_4:-70335560 Msa0518540:CDS
TATTCAGGATCAAGTTCTGA+AGG 0.553591 3_4:+70335966 None:intergenic
AAGATGGCCCCAAACAAAAG+TGG 0.554048 3_4:-70333451 Msa0518540:CDS
GCTCAATACTCTGAGCTGAA+TGG 0.555896 3_4:+70333102 None:intergenic
AGACAGGTTGAACATATACT+TGG 0.557340 3_4:+70333502 None:intergenic
AGATTGTAAGGACGAAAACC+CGG 0.560505 3_4:+70333411 None:intergenic
CTCCAAAACTGATCATGTAT+GGG 0.563692 3_4:+70334416 None:intergenic
TCTGGGATTTCTTGGAGCAG+TGG 0.566045 3_4:-70333304 Msa0518540:CDS
AATCCAGTGAGCAAGTTCCC+TGG 0.566335 3_4:+70333597 None:intergenic
TTTAACAGTTTATTATCCAG+TGG 0.568524 3_4:-70333271 Msa0518540:CDS
TGCAGGCGGGCGCCAATACA+CGG 0.575732 3_4:-70335116 Msa0518540:intron
TGCAAGATATACATCTCCAC+TGG 0.575990 3_4:+70333255 None:intergenic
TGGGGTGCTATCCTTAGCAT+GGG 0.576138 3_4:-70335585 Msa0518540:CDS
TTAGTATGTACAATCCAGCT+TGG 0.584076 3_4:-70333575 Msa0518540:CDS
AACAGCTGTGATTGGGTCTG+GGG 0.588270 3_4:-70335603 Msa0518540:CDS
GAACACAAAATTCCAATCCC+GGG 0.593016 3_4:-70333429 Msa0518540:CDS
AAGGGACACCATCTATGGAA+TGG 0.597405 3_4:+70335985 None:intergenic
CCTGAATACTTCGACGACGA+TGG 0.603286 3_4:-70335951 Msa0518540:CDS
TTGTGCCGTAGCCCATGCTA+AGG 0.606269 3_4:+70335574 None:intergenic
TGGAGATGTATATCTTGCAA+AGG 0.629124 3_4:-70333251 Msa0518540:CDS
TTGAGAGTATGAATAAGCAA+AGG 0.634614 3_4:+70333866 None:intergenic
AGATGTCTCCATTTATCAGG+AGG 0.634869 3_4:-70334466 Msa0518540:CDS
TAGGTCCTCTATAATACTGG+CGG 0.635831 3_4:+70333137 None:intergenic
CTAGCTGAATGTTATCGTTG+TGG 0.636182 3_4:-70335482 Msa0518540:CDS
CGTTGTGGAGATCCTGTGCA+TGG 0.641437 3_4:-70335467 Msa0518540:CDS
TTCCAGAGTTTCATAATACG+TGG 0.645176 3_4:-70334369 Msa0518540:CDS
CACCACGTATTATGAAACTC+TGG 0.648752 3_4:+70334367 None:intergenic
ATTCAGGATCAAGTTCTGAA+GGG 0.648974 3_4:+70335967 None:intergenic
TTCAAGGGTACTCTCACTGG+AGG 0.649496 3_4:-70333954 Msa0518540:CDS
GGGTTGCTTGACATGTGACA+TGG 0.650556 3_4:+70334436 None:intergenic
CTGAAAAGGATCATCACCAA+AGG 0.653603 3_4:+70335533 None:intergenic
TCAACAACAACAAAATTGTG+GGG 0.655949 3_4:-70333915 Msa0518540:CDS
GCTATCCTTAGCATGGGCTA+CGG 0.662463 3_4:-70335579 Msa0518540:CDS
CAGAGTTTCATAATACGTGG+TGG 0.670066 3_4:-70334366 Msa0518540:CDS
CTTGGTATGCTCCCACAAGG+TGG 0.675365 3_4:+70333520 None:intergenic
ATACTTGGTATGCTCCCACA+AGG 0.692310 3_4:+70333517 None:intergenic
ATGAAGTATACAAAGTGACG+AGG 0.719582 3_4:+70335510 None:intergenic
TCCCACAAGGTGGATTACGA+CGG 0.722373 3_4:+70333530 None:intergenic
CATTTGGAGAACAAGCACCA+GGG 0.723647 3_4:-70333614 Msa0518540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAATTAATT+CGG - chr3_4:70335094-70335113 Msa0518540:CDS 0.0%
!!! TTATATAATTTTACGTTAAA+TGG - chr3_4:70333230-70333249 Msa0518540:CDS 10.0%
!!! TTTTTTATGTCTAATTTTTA+TGG - chr3_4:70333406-70333425 Msa0518540:CDS 10.0%
!!! TTTTTATGTCTAATTTTTAT+GGG - chr3_4:70333407-70333426 Msa0518540:CDS 10.0%
!! AAAAAAAAAAAATTAATTCG+GGG - chr3_4:70335096-70335115 Msa0518540:CDS 10.0%
!!! TTTTAATTAAGATAAAGAAA+GGG + chr3_4:70335316-70335335 None:intergenic 10.0%
!!! ATTTTAATTAAGATAAAGAA+AGG + chr3_4:70335317-70335336 None:intergenic 10.0%
!!! AAAGCATGTATATTATTTTT+TGG - chr3_4:70333885-70333904 Msa0518540:CDS 15.0%
!! ATATATTAATTACCATTAGT+GGG - chr3_4:70334286-70334305 Msa0518540:intron 15.0%
!! AAAAAAAAAAAAACCATTGA+TGG - chr3_4:70334473-70334492 Msa0518540:CDS 15.0%
!!! TTGATGAGTTTATTTTAATT+TGG - chr3_4:70334609-70334628 Msa0518540:intron 15.0%
!!! TGATGAGTTTATTTTAATTT+GGG - chr3_4:70334610-70334629 Msa0518540:intron 15.0%
!!! TAAATCTATTTAGATTAGTT+TGG - chr3_4:70335732-70335751 Msa0518540:intron 15.0%
!!! TTAAATTTTCATTACTAGGA+GGG + chr3_4:70333441-70333460 None:intergenic 20.0%
!!! TGTGTTAAATTTTCATTACT+AGG + chr3_4:70333445-70333464 None:intergenic 20.0%
!!! TTTTTTTTTTGTTATGAGTC+AGG - chr3_4:70333465-70333484 Msa0518540:CDS 20.0%
!!! AATGTGTTTGTATTTAAGAT+CGG - chr3_4:70333818-70333837 Msa0518540:intron 20.0%
!! ATGCTTGTAAAAGATAATTA+CGG + chr3_4:70334125-70334144 None:intergenic 20.0%
!! GATATATTAATTACCATTAG+TGG - chr3_4:70334285-70334304 Msa0518540:intron 20.0%
!! ATAAGTATAACCTAAAAAGA+AGG - chr3_4:70334430-70334449 Msa0518540:CDS 20.0%
!! TCTTACTTCTATTATTGATA+GGG - chr3_4:70334567-70334586 Msa0518540:intron 20.0%
!!! ACTGATTTTTAAACAGAAAA+TGG - chr3_4:70335140-70335159 Msa0518540:intron 20.0%
!!! TATATATTTTTACTACACAC+AGG - chr3_4:70335373-70335392 Msa0518540:intron 20.0%
!!! ATTCTTCATCGTTTTTATTT+CGG + chr3_4:70335420-70335439 None:intergenic 20.0%
!! CTAAATAGATTTAGATTGTA+AGG + chr3_4:70335725-70335744 None:intergenic 20.0%
! AAGAAGAAATAGAAAGAAAC+CGG - chr3_4:70333111-70333130 Msa0518540:CDS 25.0%
! GAATTTCACAAGTTTACATT+TGG - chr3_4:70333324-70333343 Msa0518540:CDS 25.0%
!! GTTAAATTTTCATTACTAGG+AGG + chr3_4:70333442-70333461 None:intergenic 25.0%
! ATATAATAACAGCTGTGATT+GGG - chr3_4:70333511-70333530 Msa0518540:CDS 25.0%
! GATGTAATATTCTTAAGAGT+TGG - chr3_4:70333944-70333963 Msa0518540:CDS 25.0%
! TAGTTTCAAACATAATATGC+AGG - chr3_4:70333988-70334007 Msa0518540:intron 25.0%
! TGTAAAAGATAATTACGGTA+TGG + chr3_4:70334120-70334139 None:intergenic 25.0%
!! CAATATCAATCCTTCTTTTT+AGG + chr3_4:70334443-70334462 None:intergenic 25.0%
!!! ACATATTTTTGATCCATCAA+TGG + chr3_4:70334489-70334508 None:intergenic 25.0%
!! ACAAATGCTAATAGGTATTT+TGG + chr3_4:70334548-70334567 None:intergenic 25.0%
! GTCTTACTTCTATTATTGAT+AGG - chr3_4:70334566-70334585 Msa0518540:intron 25.0%
!!! TTTATTTTAATTTGGGTGAC+AGG - chr3_4:70334617-70334636 Msa0518540:intron 25.0%
!!! TTATTTTAATTTGGGTGACA+GGG - chr3_4:70334618-70334637 Msa0518540:intron 25.0%
! ATTCTTGATCATGTACTTTA+CGG - chr3_4:70335009-70335028 Msa0518540:intron 25.0%
! TCAAATTCTCATTGAAATTC+AGG - chr3_4:70335274-70335293 Msa0518540:intron 25.0%
! ACTGGATAATAAACTGTTAA+AGG + chr3_4:70335851-70335870 None:intergenic 25.0%
! TTTAACAGTTTATTATCCAG+TGG - chr3_4:70335850-70335869 Msa0518540:intron 25.0%
CATATAATAACAGCTGTGAT+TGG - chr3_4:70333510-70333529 Msa0518540:CDS 30.0%
! AAAGAGAAGCTATACTTTTG+TGG - chr3_4:70333677-70333696 Msa0518540:CDS 30.0%
! TTGCTTGTATTTAAGAGCAT+GGG + chr3_4:70333764-70333783 None:intergenic 30.0%
TTTGCTTGTATTTAAGAGCA+TGG + chr3_4:70333765-70333784 None:intergenic 30.0%
TTTCAAACATAATATGCAGG+CGG - chr3_4:70333991-70334010 Msa0518540:intron 30.0%
GTTCAGTACTTGTATCTTTA+TGG - chr3_4:70334039-70334058 Msa0518540:intron 30.0%
GAAGTAAGACAAATGCTAAT+AGG + chr3_4:70334556-70334575 None:intergenic 30.0%
! ATAGCTGCAATAATGTCTTT+TGG - chr3_4:70334787-70334806 Msa0518540:intron 30.0%
ATCAAACAATTGTACTCAAG+TGG - chr3_4:70334934-70334953 Msa0518540:intron 30.0%
! AGAACTTGGTTCTCTAAATT+TGG + chr3_4:70334973-70334992 None:intergenic 30.0%
TTACATCCAAAAGTAGAACT+TGG + chr3_4:70334987-70335006 None:intergenic 30.0%
!!! TGTTAACAGTCTCGTTTTTT+GGG + chr3_4:70335068-70335087 None:intergenic 30.0%
TGTCAACAACAACAAAATTG+TGG - chr3_4:70335204-70335223 Msa0518540:intron 30.0%
GTCAACAACAACAAAATTGT+GGG - chr3_4:70335205-70335224 Msa0518540:intron 30.0%
TCAACAACAACAAAATTGTG+GGG - chr3_4:70335206-70335225 Msa0518540:intron 30.0%
! TTGAGAGTATGAATAAGCAA+AGG + chr3_4:70335258-70335277 None:intergenic 30.0%
!! TTCATCGTTTTTATTTCGGA+TGG + chr3_4:70335416-70335435 None:intergenic 30.0%
!! ACAGCATTTTATTTGCTTTG+TGG - chr3_4:70335467-70335486 Msa0518540:CDS 30.0%
!! TTTGTGTTCCACTTTTGTTT+GGG + chr3_4:70335681-70335700 None:intergenic 30.0%
!! TTTTGTGTTCCACTTTTGTT+TGG + chr3_4:70335682-70335701 None:intergenic 30.0%
ACATTGCAACAAAAGTAGTT+AGG + chr3_4:70335775-70335794 None:intergenic 30.0%
TCCTTTCTTTAATGATGTTC+TGG - chr3_4:70335799-70335818 Msa0518540:intron 30.0%
CCTTTCTTTAATGATGTTCT+GGG - chr3_4:70335800-70335819 Msa0518540:intron 30.0%
!! TCCATTTAGAACTCCATTTT+GGG + chr3_4:70335901-70335920 None:intergenic 30.0%
!! ATCCATTTAGAACTCCATTT+TGG + chr3_4:70335902-70335921 None:intergenic 30.0%
!!! GAGTTCTAAATGGATTTTGT+TGG - chr3_4:70335907-70335926 Msa0518540:intron 30.0%
! ATGCTTTATTGTATCAGTTG+TGG - chr3_4:70335946-70335965 Msa0518540:CDS 30.0%
!! ATTCAGGATCAAGTTCTGAA+GGG + chr3_4:70333157-70333176 None:intergenic 35.0%
! TATTCAGGATCAAGTTCTGA+AGG + chr3_4:70333158-70333177 None:intergenic 35.0%
TAATGTGTACCTGTTCGTTT+AGG + chr3_4:70333200-70333219 None:intergenic 35.0%
GTTATGAGTCAGGAACAATA+TGG - chr3_4:70333475-70333494 Msa0518540:CDS 35.0%
ATGAAGTATACAAAGTGACG+AGG + chr3_4:70333614-70333633 None:intergenic 35.0%
TTCAAACATAATATGCAGGC+GGG - chr3_4:70333992-70334011 Msa0518540:intron 35.0%
TATCTTTATGGCAGTGCAAT+AGG - chr3_4:70334051-70334070 Msa0518540:intron 35.0%
TCAACTCACATCATGCTAAT+GGG + chr3_4:70334093-70334112 None:intergenic 35.0%
ATCAACTCACATCATGCTAA+TGG + chr3_4:70334094-70334113 None:intergenic 35.0%
!! TTGTTCTAAAGACCCACTAA+TGG + chr3_4:70334301-70334320 None:intergenic 35.0%
TCTAGATGTCTCCATTTATC+AGG - chr3_4:70334652-70334671 Msa0518540:intron 35.0%
! GATCTTTTCCTCCTGATAAA+TGG + chr3_4:70334666-70334685 None:intergenic 35.0%
! AACCCATACATGATCAGTTT+TGG - chr3_4:70334703-70334722 Msa0518540:intron 35.0%
CTCCAAAACTGATCATGTAT+GGG + chr3_4:70334708-70334727 None:intergenic 35.0%
ACTCCAAAACTGATCATGTA+TGG + chr3_4:70334709-70334728 None:intergenic 35.0%
TTCCAGAGTTTCATAATACG+TGG - chr3_4:70334752-70334771 Msa0518540:intron 35.0%
! AGAGAACCAAGTTCTACTTT+TGG - chr3_4:70334978-70334997 Msa0518540:intron 35.0%
!!! GTTAACAGTCTCGTTTTTTG+GGG + chr3_4:70335067-70335086 None:intergenic 35.0%
!!! GTGTTAACAGTCTCGTTTTT+TGG + chr3_4:70335069-70335088 None:intergenic 35.0%
AACAGAAAATGGTACCTTCA+AGG - chr3_4:70335151-70335170 Msa0518540:intron 35.0%
ACAGAAAATGGTACCTTCAA+GGG - chr3_4:70335152-70335171 Msa0518540:intron 35.0%
!! ATCGTTTTTATTTCGGATGG+TGG + chr3_4:70335413-70335432 None:intergenic 35.0%
TTAGTATGTACAATCCAGCT+TGG - chr3_4:70335546-70335565 Msa0518540:CDS 35.0%
! AGACAGGTTGAACATATACT+TGG + chr3_4:70335622-70335641 None:intergenic 35.0%
!! TTGTGTTCCACTTTTGTTTG+GGG + chr3_4:70335680-70335699 None:intergenic 35.0%
CCCAGAACATCATTAAAGAA+AGG + chr3_4:70335803-70335822 None:intergenic 35.0%
!! AATGATGTTCTGGGATTTCT+TGG - chr3_4:70335809-70335828 Msa0518540:intron 35.0%
TGGAGATGTATATCTTGCAA+AGG - chr3_4:70335870-70335889 Msa0518540:intron 35.0%
!! TCCCAAAATGGAGTTCTAAA+TGG - chr3_4:70335897-70335916 Msa0518540:intron 35.0%
CTTTAGGTCCTCTATAATAC+TGG + chr3_4:70335990-70336009 None:intergenic 35.0%
! TGAATGGCTTGTAATGCTTT+AGG + chr3_4:70336006-70336025 None:intergenic 35.0%
!! GTGTCGATCTTTTGTCTTCT+TGG - chr3_4:70333346-70333365 Msa0518540:CDS 40.0%
CTGAAAAGGATCATCACCAA+AGG + chr3_4:70333591-70333610 None:intergenic 40.0%
CTAGCTGAATGTTATCGTTG+TGG - chr3_4:70333639-70333658 Msa0518540:CDS 40.0%
CTATTCCACAAACCGTGTAT+TGG + chr3_4:70334020-70334039 None:intergenic 40.0%
AGATGTCTCCATTTATCAGG+AGG - chr3_4:70334655-70334674 Msa0518540:intron 40.0%
CACCACGTATTATGAAACTC+TGG + chr3_4:70334757-70334776 None:intergenic 40.0%
CAGAGTTTCATAATACGTGG+TGG - chr3_4:70334755-70334774 Msa0518540:intron 40.0%
!! TCTTTTGGCTATTCCACAGT+TGG - chr3_4:70334802-70334821 Msa0518540:intron 40.0%
TTAGCAATTTCCCAAACAGC+AGG - chr3_4:70334832-70334851 Msa0518540:intron 40.0%
!!! TGGGGAATTTTTCAAGCTCT+TGG - chr3_4:70335224-70335243 Msa0518540:intron 40.0%
TTATTTGCTTTGTGGCTGCA+TGG - chr3_4:70335475-70335494 Msa0518540:CDS 40.0%
TATTTGCTTTGTGGCTGCAT+GGG - chr3_4:70335476-70335495 Msa0518540:CDS 40.0%
AGAAAGGAGCAGCAAAAAGA+TGG - chr3_4:70335654-70335673 Msa0518540:intron 40.0%
GGAACACAAAATTCCAATCC+CGG - chr3_4:70335691-70335710 Msa0518540:intron 40.0%
GAACACAAAATTCCAATCCC+GGG - chr3_4:70335692-70335711 Msa0518540:intron 40.0%
AGATTGTAAGGACGAAAACC+CGG + chr3_4:70335713-70335732 None:intergenic 40.0%
TGCAAGATATACATCTCCAC+TGG + chr3_4:70335869-70335888 None:intergenic 40.0%
TGCAAAGGAAGATCCCAAAA+TGG - chr3_4:70335885-70335904 Msa0518540:intron 40.0%
TAGGTCCTCTATAATACTGG+CGG + chr3_4:70335987-70336006 None:intergenic 40.0%
AAGGGACACCATCTATGGAA+TGG + chr3_4:70333139-70333158 None:intergenic 45.0%
! TTCTGAAGGGACACCATCTA+TGG + chr3_4:70333144-70333163 None:intergenic 45.0%
ATAACAGCTGTGATTGGGTC+TGG - chr3_4:70333516-70333535 Msa0518540:CDS 45.0%
TAACAGCTGTGATTGGGTCT+GGG - chr3_4:70333517-70333536 Msa0518540:CDS 45.0%
ACAAAGTGACGAGGCTGAAA+AGG + chr3_4:70333605-70333624 None:intergenic 45.0%
TAGCTTCTCTTTCCATGCAC+AGG + chr3_4:70333669-70333688 None:intergenic 45.0%
GATGCAGTTCGTAGCATTCT+TGG - chr3_4:70333699-70333718 Msa0518540:CDS 45.0%
TGCTAAAAAGACGCCAACTG+TGG + chr3_4:70334818-70334837 None:intergenic 45.0%
! ACGACGATAACCTGCTGTTT+GGG + chr3_4:70334845-70334864 None:intergenic 45.0%
AACGACGATAACCTGCTGTT+TGG + chr3_4:70334846-70334865 None:intergenic 45.0%
!!! CAGTCTCGTTTTTTGGGGAA+AGG + chr3_4:70335062-70335081 None:intergenic 45.0%
CATTTGGAGAACAAGCACCA+GGG - chr3_4:70335507-70335526 Msa0518540:CDS 45.0%
ATACTTGGTATGCTCCCACA+AGG + chr3_4:70335607-70335626 None:intergenic 45.0%
TTCTCTACGAAGGCAAAGAC+AGG + chr3_4:70335638-70335657 None:intergenic 45.0%
GTCTTTGCCTTCGTAGAGAA+AGG - chr3_4:70335638-70335657 Msa0518540:intron 45.0%
AAGATGGCCCCAAACAAAAG+TGG - chr3_4:70335670-70335689 Msa0518540:intron 45.0%
GATTGTAAGGACGAAAACCC+GGG + chr3_4:70335712-70335731 None:intergenic 45.0%
! TTCTTGGAGCAGTGGGATTT+TGG - chr3_4:70335825-70335844 Msa0518540:intron 45.0%
CTCAACCGCCAGTATTATAG+AGG - chr3_4:70335979-70335998 Msa0518540:CDS 45.0%
!! AAAAAAAAAAAAATTAATTC+GGG - chr3_4:70335095-70335114 Msa0518540:CDS 5.0%
AACCGGAGCCATTCCATAGA+TGG - chr3_4:70333128-70333147 Msa0518540:CDS 50.0%
CACCATCTATGGAATGGCTC+CGG + chr3_4:70333133-70333152 None:intergenic 50.0%
! CCATCGTCGTCGAAGTATTC+AGG + chr3_4:70333173-70333192 None:intergenic 50.0%
CCTGAATACTTCGACGACGA+TGG - chr3_4:70333170-70333189 Msa0518540:CDS 50.0%
GATGGTCGTCCTAAACGAAC+AGG - chr3_4:70333188-70333207 Msa0518540:CDS 50.0%
AACAGCTGTGATTGGGTCTG+GGG - chr3_4:70333518-70333537 Msa0518540:CDS 50.0%
! TGGGGTGCTATCCTTAGCAT+GGG - chr3_4:70333536-70333555 Msa0518540:CDS 50.0%
! GCTATCCTTAGCATGGGCTA+CGG - chr3_4:70333542-70333561 Msa0518540:CDS 50.0%
!! ACGGCACAATTGGGTTGGAT+TGG - chr3_4:70333561-70333580 Msa0518540:CDS 50.0%
!! GCACAATTGGGTTGGATTGG+TGG - chr3_4:70333564-70333583 Msa0518540:CDS 50.0%
GGGTTGCTTGACATGTGACA+TGG + chr3_4:70334688-70334707 None:intergenic 50.0%
ACGGTAACTCGAACGAACTC+AGG - chr3_4:70335028-70335047 Msa0518540:intron 50.0%
!! ACCTTCAAGGGTACTCTCAC+TGG - chr3_4:70335164-70335183 Msa0518540:intron 50.0%
!! TCCAGTGAGAGTACCCTTGA+AGG + chr3_4:70335168-70335187 None:intergenic 50.0%
!! TTCAAGGGTACTCTCACTGG+AGG - chr3_4:70335167-70335186 Msa0518540:intron 50.0%
! TGCATGGGCTATGCTGCATT+TGG - chr3_4:70335491-70335510 Msa0518540:CDS 50.0%
GCATTTGGAGAACAAGCACC+AGG - chr3_4:70335506-70335525 Msa0518540:CDS 50.0%
AATCCAGTGAGCAAGTTCCC+TGG + chr3_4:70335527-70335546 None:intergenic 50.0%
! TCCAGCTTGGCTCATCGATT+TGG - chr3_4:70335559-70335578 Msa0518540:CDS 50.0%
!! TCCCACAAGGTGGATTACGA+CGG + chr3_4:70335594-70335613 None:intergenic 50.0%
TGCTGCTCCTTTCTCTACGA+AGG + chr3_4:70335648-70335667 None:intergenic 50.0%
TAAGGACGAAAACCCGGGAT+TGG + chr3_4:70335707-70335726 None:intergenic 50.0%
! TCTGGGATTTCTTGGAGCAG+TGG - chr3_4:70335817-70335836 Msa0518540:intron 50.0%
CTGGGATTTCTTGGAGCAGT+GGG - chr3_4:70335818-70335837 Msa0518540:intron 50.0%
GTATCAGTTGTGGCTGCTCT+TGG - chr3_4:70335956-70335975 Msa0518540:CDS 50.0%
! CTGGGGTGCTATCCTTAGCA+TGG - chr3_4:70333535-70333554 Msa0518540:CDS 55.0%
TTGTGCCGTAGCCCATGCTA+AGG + chr3_4:70333550-70333569 None:intergenic 55.0%
! AGCATGGGCTACGGCACAAT+TGG - chr3_4:70333551-70333570 Msa0518540:CDS 55.0%
! GCATGGGCTACGGCACAATT+GGG - chr3_4:70333552-70333571 Msa0518540:CDS 55.0%
!! GGGTTGGATTGGTGGTCCTT+TGG - chr3_4:70333572-70333591 Msa0518540:CDS 55.0%
CGTTGTGGAGATCCTGTGCA+TGG - chr3_4:70333654-70333673 Msa0518540:CDS 55.0%
GCCAAATCGATGAGCCAAGC+TGG + chr3_4:70335563-70335582 None:intergenic 55.0%
TGCCGTCGTAATCCACCTTG+TGG - chr3_4:70335589-70335608 Msa0518540:CDS 55.0%
GCCGTCGTAATCCACCTTGT+GGG - chr3_4:70335590-70335609 Msa0518540:CDS 55.0%
CTTGGTATGCTCCCACAAGG+TGG + chr3_4:70335604-70335623 None:intergenic 55.0%
GGGCTACGGCACAATTGGGT+TGG - chr3_4:70333556-70333575 Msa0518540:CDS 60.0%
GGGCGCCAATACACGGTTTG+TGG - chr3_4:70334012-70334031 Msa0518540:intron 60.0%
! GCACCAGGGAACTTGCTCAC+TGG - chr3_4:70335521-70335540 Msa0518540:CDS 60.0%
TGCAGGCGGGCGCCAATACA+CGG - chr3_4:70334005-70334024 Msa0518540:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 70333104 70336039 70333104 ID=Msa0518540;Name=Msa0518540
chr3_4 mRNA 70333104 70336039 70333104 ID=Msa0518540-mRNA-1;Parent=Msa0518540;Name=Msa0518540-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|6|0|477
chr3_4 exon 70335934 70336039 70335934 ID=Msa0518540-mRNA-1:exon:19043;Parent=Msa0518540-mRNA-1
chr3_4 exon 70335423 70335656 70335423 ID=Msa0518540-mRNA-1:exon:19042;Parent=Msa0518540-mRNA-1
chr3_4 exon 70335040 70335133 70335040 ID=Msa0518540-mRNA-1:exon:19041;Parent=Msa0518540-mRNA-1
chr3_4 exon 70334290 70334504 70334290 ID=Msa0518540-mRNA-1:exon:19040;Parent=Msa0518540-mRNA-1
chr3_4 exon 70333848 70333987 70333848 ID=Msa0518540-mRNA-1:exon:19039;Parent=Msa0518540-mRNA-1
chr3_4 exon 70333104 70333748 70333104 ID=Msa0518540-mRNA-1:exon:19038;Parent=Msa0518540-mRNA-1
chr3_4 CDS 70335934 70336039 70335934 ID=Msa0518540-mRNA-1:cds;Parent=Msa0518540-mRNA-1
chr3_4 CDS 70335423 70335656 70335423 ID=Msa0518540-mRNA-1:cds;Parent=Msa0518540-mRNA-1
chr3_4 CDS 70335040 70335133 70335040 ID=Msa0518540-mRNA-1:cds;Parent=Msa0518540-mRNA-1
chr3_4 CDS 70334290 70334504 70334290 ID=Msa0518540-mRNA-1:cds;Parent=Msa0518540-mRNA-1
chr3_4 CDS 70333848 70333987 70333848 ID=Msa0518540-mRNA-1:cds;Parent=Msa0518540-mRNA-1
chr3_4 CDS 70333104 70333748 70333104 ID=Msa0518540-mRNA-1:cds;Parent=Msa0518540-mRNA-1
Gene Sequence

>Msa0518540

ATGTTGAAAGAAGAAATAGAAAGAAACCGGAGCCATTCCATAGATGGTGTCCCTTCAGAACTTGATCCTGAATACTTCGACGACGATGGTCGTCCTAAACGAACAGGAACAATATGGACAACAAGTTCTCATATAATAACAGCTGTGATTGGGTCTGGGGTGCTATCCTTAGCATGGGCTACGGCACAATTGGGTTGGATTGGTGGTCCTTTGGTGATGATCCTTTTCAGCCTCGTCACTTTGTATACTTCATCAATGCTAGCTGAATGTTATCGTTGTGGAGATCCTGTGCATGGAAAGAGAAGCTATACTTTTGTGGATGCAGTTCGTAGCATTCTTGGCGGGCGCCAATACACGGTTTGTGGAATAGTTCAGTACTTGTATCTTTATGGCAGTGCAATAGGATACTCGATTGCAGCTCCCATTAGCATGATGGAAATAAACAAGTCTAGATGTCTCCATTTATCAGGAGGAAAAGATCCATGTCACATGTCAAGCAACCCATACATGATCAGTTTTGGAGTAATCGAAATTTTCGTTTCTCAAATTCCAGAGTTTCATAATACGTGGTGGCTCTCAGTAATAGCTGCAATAATGTCTTTTGGCTATTCCACAGTTGGCGTCTTTTTAGCAATTTCCCAAACAGCAGAAAATGGTACCTTCAAGGGTACTCTCACTGGAGGAAGCACTGAAGATGTGTCAACAACAACAAAATTGTGGGGAATTTTTCAAGCTCTTGGTGACATAGCCTTTGCTTATTCATACTCTCAAATTCTCATTGAAATTCAGGACACTATAAAAAGTCCACCATCCGAAATAAAAACGATGAAGAATGCAGCAGCTTTAAGTGTTGCTGTCACTACAGCATTTTATTTGCTTTGTGGCTGCATGGGCTATGCTGCATTTGGAGAACAAGCACCAGGGAACTTGCTCACTGGATTTAGTATGTACAATCCAGCTTGGCTCATCGATTTGGCGAATGCTGCCGTCGTAATCCACCTTGTGGGAGCATACCAAGTATATGTTCAACCTGTCTTTGCCTTCGTAGAGAAAGGAGCAGCAAAAAGATGGCCCCAAACAAAAGTGGAACACAAAATTCCAATCCCGGGTTTTCGTCCTTACAATCTAAATCTATTTAGATTAGTTTGGAGAACAGTATTTATGATCCTAACTACTTTTGTTGCAATGTTGATTCCTTTCTTTAATGATGTTCTGGGATTTCTTGGAGCAGTGGGATTTTGGCCTTTAACAGTTTATTATCCAGTGGAGATGTATATCTTGCAAAGGAAGATCCCAAAATGGAGTTCTAAATGGATTTTGTTGGAAATCATAAGTGTTATATGCTTTATTGTATCAGTTGTGGCTGCTCTTGGCTCAACCGCCAGTATTATAGAGGACCTAAAGCATTACAAGCCATTCAGCTCAGAGTATTGA
Protein sequence

>Msa0518540

MLKEEIERNRSHSIDGVPSELDPEYFDDDGRPKRTGTIWTTSSHIITAVIGSGVLSLAWATAQLGWIGGPLVMILFSLVTLYTSSMLAECYRCGDPVHGKRSYTFVDAVRSILGGRQYTVCGIVQYLYLYGSAIGYSIAAPISMMEINKSRCLHLSGGKDPCHMSSNPYMISFGVIEIFVSQIPEFHNTWWLSVIAAIMSFGYSTVGVFLAISQTAENGTFKGTLTGGSTEDVSTTTKLWGIFQALGDIAFAYSYSQILIEIQDTIKSPPSEIKTMKNAAALSVAVTTAFYLLCGCMGYAAFGEQAPGNLLTGFSMYNPAWLIDLANAAVVIHLVGAYQVYVQPVFAFVEKGAAKRWPQTKVEHKIPIPGFRPYNLNLFRLVWRTVFMILTTFVAMLIPFFNDVLGFLGAVGFWPLTVYYPVEMYILQRKIPKWSSKWILLEIISVICFIVSVVAALGSTASIIEDLKHYKPFSSEY*