AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0428000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0428000 MtrunA17_Chr3g0130641 93.699 365 23 0 1 365 1 365 0.0 709
Msa0428000 MtrunA17_Chr3g0130651 71.060 349 99 1 11 359 4 350 0.0 516
Msa0428000 MtrunA17_Chr8g0384661 68.328 341 108 0 27 367 44 384 7.57e-170 483
Msa0428000 MtrunA17_Chr3g0125091 62.943 367 132 3 1 365 1 365 6.32e-168 478
Msa0428000 MtrunA17_Chr3g0125081 61.749 366 137 2 1 365 1 364 1.42e-167 477
Msa0428000 MtrunA17_Chr1g0159761 66.176 340 115 0 26 365 20 359 7.40e-167 475
Msa0428000 MtrunA17_Chr3g0125101 60.763 367 140 3 1 365 1 365 2.76e-162 463
Msa0428000 MtrunA17_Chr4g0037201 60.163 369 138 2 5 364 28 396 1.45e-161 462
Msa0428000 MtrunA17_Chr3g0140761 53.784 370 161 6 1 366 1 364 2.90e-141 410
Msa0428000 MtrunA17_Chr1g0146491 55.085 354 149 5 18 366 6 354 2.42e-140 407
Msa0428000 MtrunA17_Chr1g0146411 49.041 365 184 2 1 365 1 363 3.23e-125 369
Msa0428000 MtrunA17_Chr3g0103231 49.563 343 171 1 26 368 16 356 6.55e-124 365
Msa0428000 MtrunA17_Chr3g0103211 50.877 342 166 1 26 367 16 355 1.60e-121 359
Msa0428000 MtrunA17_Chr1g0206871 50.729 343 158 3 33 365 15 356 2.67e-120 356
Msa0428000 MtrunA17_Chr1g0206821 46.517 402 153 4 26 365 15 416 1.89e-118 353
Msa0428000 MtrunA17_Chr3g0103271 51.282 351 168 2 10 359 4 352 4.46e-118 350
Msa0428000 MtrunA17_Chr5g0402151 60.339 295 84 3 36 330 3 264 3.14e-117 341
Msa0428000 MtrunA17_Chr3g0103221 48.225 338 173 1 30 367 27 362 4.57e-117 348
Msa0428000 MtrunA17_Chr5g0447221 45.628 366 192 4 2 366 1 360 2.64e-113 338
Msa0428000 MtrunA17_Chr3g0130671 64.935 231 81 0 16 246 22 252 2.81e-109 321
Msa0428000 MtrunA17_Chr3g0140531 46.686 347 176 3 24 365 15 357 4.21e-109 327
Msa0428000 MtrunA17_Chr3g0140541 48.851 348 173 2 18 365 14 356 2.19e-108 325
Msa0428000 MtrunA17_Chr1g0146331 45.266 338 181 2 27 362 14 349 6.32e-108 324
Msa0428000 MtrunA17_Chr1g0146391 46.471 340 173 3 27 362 6 340 1.90e-107 323
Msa0428000 MtrunA17_Chr2g0293331 45.087 346 186 3 21 365 1 343 3.70e-106 319
Msa0428000 MtrunA17_Chr5g0447231 47.734 331 170 2 33 362 25 353 2.91e-100 305
Msa0428000 MtrunA17_Chr1g0206851 48.031 254 124 3 114 365 19 266 6.59e-78 244
Msa0428000 MtrunA17_Chr3g0103251 44.622 251 108 2 147 368 21 269 2.72e-70 224
Msa0428000 MtrunA17_Chr1g0206831 47.826 230 112 2 138 365 6 229 5.73e-67 215
Msa0428000 MtrunA17_Chr5g0422741 33.611 360 186 14 20 363 15 337 5.02e-42 152
Msa0428000 MtrunA17_Chr1g0198531 31.105 344 214 8 13 351 3 328 2.48e-39 145
Msa0428000 MtrunA17_Chr3g0103261 49.600 125 63 0 26 150 16 140 8.67e-38 133
Msa0428000 MtrunA17_Chr7g0230011 30.448 335 210 8 28 356 22 339 9.38e-37 138
Msa0428000 MtrunA17_Chr1g0206841 62.105 95 36 0 20 114 8 102 1.06e-36 129
Msa0428000 MtrunA17_Chr2g0331721 29.235 366 220 9 4 356 18 357 1.15e-36 138
Msa0428000 MtrunA17_Chr1g0198541 29.970 337 192 8 29 350 20 327 2.95e-36 137
Msa0428000 MtrunA17_Chr6g0458931 30.746 335 196 8 33 356 42 351 9.90e-36 135
Msa0428000 MtrunA17_Chr4g0057851 29.641 334 199 8 34 356 11 319 1.91e-34 131
Msa0428000 MtrunA17_Chr1g0206861 52.500 120 56 1 20 138 8 127 2.18e-34 124
Msa0428000 MtrunA17_Chr6g0458921 29.573 328 197 8 34 351 15 318 4.80e-34 130
Msa0428000 MtrunA17_Chr6g0459031 30.240 334 204 10 27 351 5 318 6.64e-34 130
Msa0428000 MtrunA17_Chr3g0077651 31.889 323 198 8 34 350 53 359 2.53e-32 126
Msa0428000 MtrunA17_Chr3g0122921 27.273 352 231 9 4 350 5 336 1.28e-31 124
Msa0428000 MtrunA17_Chr1g0146351 56.000 100 43 1 43 142 13 111 4.41e-31 114
Msa0428000 MtrunA17_Chr6g0459001 29.808 312 197 8 33 340 22 315 4.48e-31 123
Msa0428000 MtrunA17_Chr6g0459021 27.401 354 229 11 5 350 4 337 6.14e-31 122
Msa0428000 MtrunA17_Chr2g0328081 28.994 338 203 9 27 351 5 318 9.34e-31 121
Msa0428000 MtrunA17_Chr8g0376991 27.811 338 215 9 28 356 15 332 2.25e-30 120
Msa0428000 MtrunA17_Chr6g0459011 29.936 314 194 9 49 356 1 294 2.50e-27 111
Msa0428000 MtrunA17_Chr1g0184721 26.062 353 235 11 27 375 16 346 1.47e-26 110
Msa0428000 MtrunA17_Chr4g0027241 26.648 349 207 13 27 351 25 348 4.72e-25 105
Msa0428000 MtrunA17_Chr1g0198081 28.221 326 202 14 44 357 70 375 7.99e-24 102
Msa0428000 MtrunA17_Chr3g0130661 69.841 63 19 0 297 359 1 63 6.07e-23 94.7
Msa0428000 MtrunA17_Chr4g0038711 26.331 357 235 12 11 350 45 390 5.14e-22 97.4
Msa0428000 MtrunA17_Chr8g0387941 23.936 376 246 13 2 363 1 350 1.56e-18 86.7
Msa0428000 MtrunA17_Chr3g0143701 24.176 364 224 13 27 362 75 414 7.71e-15 75.9
Msa0428000 MtrunA17_Chr1g0159751 55.769 52 23 0 276 327 1 52 1.16e-14 68.6
Msa0428000 MtrunA17_Chr5g0406351 23.387 372 247 12 19 371 93 445 2.07e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0428000 AT5G09220.1 66.017 359 121 1 13 370 25 383 7.06e-179 506
Msa0428000 AT1G77380.1 66.189 349 118 0 17 365 13 361 1.54e-174 494
Msa0428000 AT5G63850.1 67.647 340 110 0 26 365 11 350 2.55e-174 494
Msa0428000 AT1G44100.1 58.611 360 142 2 12 365 7 365 1.44e-159 457
Msa0428000 AT1G10010.2 53.582 349 160 2 27 373 22 370 1.98e-136 398
Msa0428000 AT1G10010.1 53.582 349 160 2 27 373 21 369 2.02e-136 398
Msa0428000 AT1G58360.1 54.203 345 156 2 22 364 24 368 1.98e-135 395
Msa0428000 AT5G49630.1 57.602 342 142 3 26 364 25 366 1.67e-134 393
Msa0428000 AT5G23810.1 48.209 363 177 5 7 366 5 359 8.21e-109 327
Msa0428000 AT5G23810.2 49.245 331 159 4 18 345 14 338 7.81e-103 308
Msa0428000 AT5G23810.4 46.586 249 124 4 18 263 14 256 2.87e-69 219
Msa0428000 AT5G23810.5 45.972 211 105 4 7 214 5 209 1.20e-54 180
Msa0428000 AT5G23810.3 45.972 211 105 4 7 214 5 209 1.20e-54 180
Msa0428000 AT1G24400.1 28.895 353 215 11 28 369 20 347 8.01e-35 133
Msa0428000 AT5G40780.1 31.153 321 199 8 34 350 34 336 2.22e-34 132
Msa0428000 AT1G67640.1 29.296 355 223 10 28 375 20 353 8.41e-33 127
Msa0428000 AT1G48640.1 30.337 356 221 10 7 356 15 349 1.37e-32 127
Msa0428000 AT1G25530.1 30.330 333 192 9 32 351 26 331 2.13e-32 126
Msa0428000 AT1G71680.2 28.415 366 215 12 7 355 8 343 5.07e-31 121
Msa0428000 AT5G40780.3 30.769 312 194 8 49 356 1 294 2.59e-30 120
Msa0428000 AT5G40780.2 30.769 312 194 8 49 356 1 294 2.59e-30 120
Msa0428000 AT1G71680.1 28.415 366 215 12 7 355 8 343 3.31e-30 120
Msa0428000 AT1G61270.1 28.453 362 228 12 22 375 19 357 1.81e-29 118
Msa0428000 AT3G01760.1 26.171 363 212 9 16 350 3 337 1.87e-29 118
Msa0428000 AT1G08230.2 27.405 343 220 12 33 365 27 350 3.15e-18 86.3
Msa0428000 AT1G47670.1 25.356 351 225 11 19 352 79 409 1.98e-14 75.1
Msa0428000 AT4G35180.1 25.357 280 189 5 34 309 58 321 3.15e-14 74.3
Msa0428000 AT4G35180.2 25.362 276 186 5 34 305 58 317 1.01e-13 72.0
Msa0428000 AT5G41800.1 24.850 334 218 10 33 354 26 338 1.08e-12 69.3
Msa0428000 AT2G39890.2 23.701 308 207 11 64 367 63 346 2.29e-12 68.6
Msa0428000 AT2G39890.1 23.701 308 207 11 64 367 63 346 2.29e-12 68.6
Msa0428000 AT2G36590.2 24.260 338 223 13 9 340 2 312 2.95e-12 67.4
Msa0428000 AT2G39890.3 23.701 308 207 11 64 367 4 287 4.28e-12 67.4

Find 91 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCAGTACTTGTATCTTTA+TGG 0.129160 3_2:+78733242 Msa0428000:CDS
TCAAATTCTCATTGAAATTC+AGG 0.231508 3_2:+78734467 Msa0428000:CDS
CCATCGTCGTCGAAGTATTC+AGG 0.273974 3_2:-78732375 None:intergenic
TCCAGCTTGGCTCATCGATT+TGG 0.332874 3_2:+78734752 Msa0428000:CDS
TATCTTTATGGCAGTGCAAT+AGG 0.337033 3_2:+78733254 Msa0428000:CDS
ATAGCTGCAATAATGTCTTT+TGG 0.346313 3_2:+78733982 Msa0428000:CDS
AGCATGGGCTACGGCACAAT+TGG 0.349061 3_2:+78732754 Msa0428000:CDS
TCTAGATGTCTCCATTTATC+AGG 0.357700 3_2:+78733847 Msa0428000:CDS
AACGACGATAACCTGCTGTT+TGG 0.358251 3_2:-78734038 None:intergenic
AACCCATACATGATCAGTTT+TGG 0.359942 3_2:+78733898 Msa0428000:CDS
ATCAACTCACATCATGCTAA+TGG 0.372993 3_2:-78733294 None:intergenic
TTCTGAAGGGACACCATCTA+TGG 0.373125 3_2:-78732346 None:intergenic
TAATGTGTACCTGTTCGTTT+AGG 0.376426 3_2:-78732402 None:intergenic
TGCATGGGCTATGCTGCATT+TGG 0.377828 3_2:+78734684 Msa0428000:CDS
CTAAATAGATTTAGATTGTA+AGG 0.384304 3_2:-78734914 None:intergenic
GATGCAGTTCGTAGCATTCT+TGG 0.385053 3_2:+78732902 Msa0428000:CDS
CATATAATAACAGCTGTGAT+TGG 0.385307 3_2:+78732713 Msa0428000:CDS
GGGTTGGATTGGTGGTCCTT+TGG 0.399149 3_2:+78732775 Msa0428000:CDS
ATAACAGCTGTGATTGGGTC+TGG 0.401374 3_2:+78732719 Msa0428000:CDS
GTTATGAGTCAGGAACAATA+TGG 0.405471 3_2:+78732678 Msa0428000:intron
ACTCCAAAACTGATCATGTA+TGG 0.410066 3_2:-78733901 None:intergenic
ACGACGATAACCTGCTGTTT+GGG 0.418197 3_2:-78734037 None:intergenic
ACAAAGTGACGAGGCTGAAA+AGG 0.428865 3_2:-78732805 None:intergenic
TTATTTGCTTTGTGGCTGCA+TGG 0.440989 3_2:+78734668 Msa0428000:CDS
ACCTTCAAGGGTACTCTCAC+TGG 0.442148 3_2:+78734357 Msa0428000:CDS
AACAGAAAATGGTACCTTCA+AGG 0.444400 3_2:+78734344 Msa0428000:intron
AACCGGAGCCATTCCATAGA+TGG 0.448209 3_2:+78732333 Msa0428000:CDS
CTGGGGTGCTATCCTTAGCA+TGG 0.450709 3_2:+78732738 Msa0428000:CDS
GTCTTTGCCTTCGTAGAGAA+AGG 0.451734 3_2:+78734831 Msa0428000:CDS
TCAACTCACATCATGCTAAT+GGG 0.455025 3_2:-78733293 None:intergenic
AAGAAGAAATAGAAAGAAAC+CGG 0.458125 3_2:+78732316 Msa0428000:CDS
CTATTCCACAAACCGTGTAT+TGG 0.465036 3_2:-78733220 None:intergenic
GCATGGGCTACGGCACAATT+GGG 0.465627 3_2:+78732755 Msa0428000:CDS
TCCAGTGAGAGTACCCTTGA+AGG 0.465923 3_2:-78734358 None:intergenic
GCACAATTGGGTTGGATTGG+TGG 0.466018 3_2:+78732767 Msa0428000:CDS
TAAGGACGAAAACCCGGGAT+TGG 0.471537 3_2:-78734896 None:intergenic
GTCAACAACAACAAAATTGT+GGG 0.475137 3_2:+78734398 Msa0428000:CDS
GCCAAATCGATGAGCCAAGC+TGG 0.477067 3_2:-78734753 None:intergenic
GGGCGCCAATACACGGTTTG+TGG 0.479067 3_2:+78733215 Msa0428000:CDS
TATTTGCTTTGTGGCTGCAT+GGG 0.481138 3_2:+78734669 Msa0428000:CDS
TAGCTTCTCTTTCCATGCAC+AGG 0.488301 3_2:-78732869 None:intergenic
ATATAATAACAGCTGTGATT+GGG 0.488499 3_2:+78732714 Msa0428000:CDS
GGGCTACGGCACAATTGGGT+TGG 0.489975 3_2:+78732759 Msa0428000:CDS
GAGAAAGGAGCAGCAAAAGA+TGG 0.496824 3_2:+78734846 Msa0428000:CDS
TGCTGCTCCTTTCTCTACGA+AGG 0.498588 3_2:-78734838 None:intergenic
GCCGTCGTAATCCACCTTGT+GGG 0.501113 3_2:+78734783 Msa0428000:CDS
GCACCAGGGAACTTGCTCAC+TGG 0.503377 3_2:+78734714 Msa0428000:CDS
TTCTCTACGAAGGCAAAGAC+AGG 0.507161 3_2:-78734828 None:intergenic
GATGGTCGTCCTAAACGAAC+AGG 0.514219 3_2:+78732393 Msa0428000:CDS
TTAGCAATTTCCCAAACAGC+AGG 0.515563 3_2:+78734027 Msa0428000:CDS
TAACAGCTGTGATTGGGTCT+GGG 0.516920 3_2:+78732720 Msa0428000:CDS
ACAGAAAATGGTACCTTCAA+GGG 0.518566 3_2:+78734345 Msa0428000:intron
TGTCAACAACAACAAAATTG+TGG 0.522475 3_2:+78734397 Msa0428000:CDS
GATTGTAAGGACGAAAACCC+GGG 0.523575 3_2:-78734901 None:intergenic
GGAACACAAAATTCCAATCC+CGG 0.528501 3_2:+78734883 Msa0428000:CDS
GCATTTGGAGAACAAGCACC+AGG 0.529176 3_2:+78734699 Msa0428000:CDS
CACCATCTATGGAATGGCTC+CGG 0.534833 3_2:-78732335 None:intergenic
TGCCGTCGTAATCCACCTTG+TGG 0.547983 3_2:+78734782 Msa0428000:CDS
ACGGCACAATTGGGTTGGAT+TGG 0.551307 3_2:+78732764 Msa0428000:CDS
TATTCAGGATCAAGTTCTGA+AGG 0.553591 3_2:-78732360 None:intergenic
AAGATGGCCCCAAACAAAAG+TGG 0.554048 3_2:+78734862 Msa0428000:CDS
AGACAGGTTGAACATATACT+TGG 0.557340 3_2:-78734812 None:intergenic
TGCAGGCGGGCGCCAATACA+CGG 0.557733 3_2:+78733208 Msa0428000:intron
AGATTGTAAGGACGAAAACC+CGG 0.560505 3_2:-78734902 None:intergenic
CTCCAAAACTGATCATGTAT+GGG 0.563692 3_2:-78733900 None:intergenic
AATCCAGTGAGCAAGTTCCC+TGG 0.566335 3_2:-78734717 None:intergenic
TGGGGTGCTATCCTTAGCAT+GGG 0.576138 3_2:+78732739 Msa0428000:CDS
TTAGTATGTACAATCCAGCT+TGG 0.584076 3_2:+78734739 Msa0428000:CDS
AACAGCTGTGATTGGGTCTG+GGG 0.588270 3_2:+78732721 Msa0428000:CDS
GAACACAAAATTCCAATCCC+GGG 0.593016 3_2:+78734884 Msa0428000:CDS
AAGGGACACCATCTATGGAA+TGG 0.597405 3_2:-78732341 None:intergenic
CCTGAATACTTCGACGACGA+TGG 0.603286 3_2:+78732375 Msa0428000:CDS
TTGTGCCGTAGCCCATGCTA+AGG 0.606269 3_2:-78732750 None:intergenic
TTGAGAGTATGAATAAGCAA+AGG 0.634614 3_2:-78734448 None:intergenic
AGATGTCTCCATTTATCAGG+AGG 0.634869 3_2:+78733850 Msa0428000:CDS
CTAGCTGAATGTTATCGTTG+TGG 0.636182 3_2:+78732842 Msa0428000:CDS
CGTTGTGGAGATCCTGTGCA+TGG 0.641437 3_2:+78732857 Msa0428000:CDS
TTCCAGAGTTTCATAATACG+TGG 0.645176 3_2:+78733947 Msa0428000:CDS
CACCACGTATTATGAAACTC+TGG 0.648752 3_2:-78733949 None:intergenic
ATTCAGGATCAAGTTCTGAA+GGG 0.648974 3_2:-78732359 None:intergenic
TTCAAGGGTACTCTCACTGG+AGG 0.649496 3_2:+78734360 Msa0428000:CDS
GGGTTGCTTGACATGTGACA+TGG 0.650556 3_2:-78733880 None:intergenic
CTGAAAAGGATCATCACCAA+AGG 0.653603 3_2:-78732791 None:intergenic
TCAACAACAACAAAATTGTG+GGG 0.655949 3_2:+78734399 Msa0428000:CDS
GCTATCCTTAGCATGGGCTA+CGG 0.662463 3_2:+78732745 Msa0428000:CDS
CAGAGTTTCATAATACGTGG+TGG 0.670066 3_2:+78733950 Msa0428000:CDS
CTTGGTATGCTCCCACAAGG+TGG 0.675365 3_2:-78734794 None:intergenic
ATACTTGGTATGCTCCCACA+AGG 0.692310 3_2:-78734797 None:intergenic
ATGAAGTATACAAAGTGACG+AGG 0.719582 3_2:-78732814 None:intergenic
TCCCACAAGGTGGATTACGA+CGG 0.722373 3_2:-78734784 None:intergenic
CATTTGGAGAACAAGCACCA+GGG 0.723647 3_2:+78734700 Msa0428000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAATTAATT+CGG + chr3_2:78734287-78734306 Msa0428000:intron 0.0%
!!! TTATATAATTTTACGTTAAA+TGG + chr3_2:78732435-78732454 Msa0428000:intron 10.0%
!!! TTTTTTATGTCTAATTTTTA+TGG + chr3_2:78732610-78732629 Msa0428000:intron 10.0%
!!! TTTTTATGTCTAATTTTTAT+GGG + chr3_2:78732611-78732630 Msa0428000:intron 10.0%
!! AAAAAAAAAAAATTAATTCG+GGG + chr3_2:78734289-78734308 Msa0428000:intron 10.0%
!!! TTTTAATTAAGATAAAGAAA+GGG - chr3_2:78734509-78734528 None:intergenic 10.0%
!!! ATTTTAATTAAGATAAAGAA+AGG - chr3_2:78734510-78734529 None:intergenic 10.0%
!!! AAAGCATGTATATTATTTTT+TGG + chr3_2:78733088-78733107 Msa0428000:intron 15.0%
!! ATATATTAATTACCATTAGT+GGG + chr3_2:78733487-78733506 Msa0428000:intron 15.0%
!! AAAAAAAAAAAAACCATTGA+TGG + chr3_2:78733668-78733687 Msa0428000:intron 15.0%
!!! TTGATGAGTTTATTTTAATT+TGG + chr3_2:78733804-78733823 Msa0428000:intron 15.0%
!!! TGATGAGTTTATTTTAATTT+GGG + chr3_2:78733805-78733824 Msa0428000:intron 15.0%
!!! TTAAATTTTCATTACTAGGA+GGG - chr3_2:78732645-78732664 None:intergenic 20.0%
!!! TGTGTTAAATTTTCATTACT+AGG - chr3_2:78732649-78732668 None:intergenic 20.0%
!!! ATTTTTTTTTGTTATGAGTC+AGG + chr3_2:78732668-78732687 Msa0428000:intron 20.0%
!!! AATGTGTTTGTATTTAAGAT+CGG + chr3_2:78733021-78733040 Msa0428000:intron 20.0%
!! ATGCTTGTAAAAGATAATTA+CGG - chr3_2:78733328-78733347 None:intergenic 20.0%
!! GATATATTAATTACCATTAG+TGG + chr3_2:78733486-78733505 Msa0428000:intron 20.0%
!! ATAAGTATAACCTAAAAAGA+AGG + chr3_2:78733631-78733650 Msa0428000:intron 20.0%
!! TCTTACTTCTATTATTGATA+GGG + chr3_2:78733762-78733781 Msa0428000:intron 20.0%
!!! ACTGATTTTTAAACAGAAAA+TGG + chr3_2:78734333-78734352 Msa0428000:intron 20.0%
!!! TATATATTTTTACTACACAC+AGG + chr3_2:78734566-78734585 Msa0428000:intron 20.0%
!!! ATTCTTCATCGTTTTTATTT+CGG - chr3_2:78734613-78734632 None:intergenic 20.0%
! AAGAAGAAATAGAAAGAAAC+CGG + chr3_2:78732316-78732335 Msa0428000:CDS 25.0%
! GAATTTCACAAGTTTACATT+TGG + chr3_2:78732528-78732547 Msa0428000:intron 25.0%
!! GTTAAATTTTCATTACTAGG+AGG - chr3_2:78732646-78732665 None:intergenic 25.0%
! ATATAATAACAGCTGTGATT+GGG + chr3_2:78732714-78732733 Msa0428000:CDS 25.0%
! GATGTAATATTCTTAAGAGT+TGG + chr3_2:78733147-78733166 Msa0428000:intron 25.0%
!! TAGTTTCAAACATAATTTGC+AGG + chr3_2:78733191-78733210 Msa0428000:intron 25.0%
! TGTAAAAGATAATTACGGTA+TGG - chr3_2:78733323-78733342 None:intergenic 25.0%
!! CAATATCAATCCTTCTTTTT+AGG - chr3_2:78733644-78733663 None:intergenic 25.0%
!!! ACATATTTTTGATCCATCAA+TGG - chr3_2:78733684-78733703 None:intergenic 25.0%
!! ACAAATGCTAATAGGTATTT+TGG - chr3_2:78733743-78733762 None:intergenic 25.0%
! GTCTTACTTCTATTATTGAT+AGG + chr3_2:78733761-78733780 Msa0428000:intron 25.0%
!!! TTTATTTTAATTTGGGTGAC+AGG + chr3_2:78733812-78733831 Msa0428000:intron 25.0%
!!! TTATTTTAATTTGGGTGACA+GGG + chr3_2:78733813-78733832 Msa0428000:intron 25.0%
! ATTCTTGATCATGTACTTTA+CGG + chr3_2:78734204-78734223 Msa0428000:intron 25.0%
! TCAAATTCTCATTGAAATTC+AGG + chr3_2:78734467-78734486 Msa0428000:CDS 25.0%
CATATAATAACAGCTGTGAT+TGG + chr3_2:78732713-78732732 Msa0428000:CDS 30.0%
! AAAGAGAAGCTATACTTTTG+TGG + chr3_2:78732880-78732899 Msa0428000:CDS 30.0%
! TTGCTTGTATTTAAGAGCAT+GGG - chr3_2:78732967-78732986 None:intergenic 30.0%
TTTGCTTGTATTTAAGAGCA+TGG - chr3_2:78732968-78732987 None:intergenic 30.0%
! TTTCAAACATAATTTGCAGG+CGG + chr3_2:78733194-78733213 Msa0428000:intron 30.0%
GTTCAGTACTTGTATCTTTA+TGG + chr3_2:78733242-78733261 Msa0428000:CDS 30.0%
GAAGTAAGACAAATGCTAAT+AGG - chr3_2:78733751-78733770 None:intergenic 30.0%
! ATAGCTGCAATAATGTCTTT+TGG + chr3_2:78733982-78734001 Msa0428000:CDS 30.0%
ATCAAACAATTGTACTCAAG+TGG + chr3_2:78734129-78734148 Msa0428000:intron 30.0%
! AGAACTTGGTTCTCTAAATT+TGG - chr3_2:78734168-78734187 None:intergenic 30.0%
TTACATCCAAAAGTAGAACT+TGG - chr3_2:78734182-78734201 None:intergenic 30.0%
!!! TGTTAACAGTCTCGTTTTTT+GGG - chr3_2:78734262-78734281 None:intergenic 30.0%
TGTCAACAACAACAAAATTG+TGG + chr3_2:78734397-78734416 Msa0428000:CDS 30.0%
GTCAACAACAACAAAATTGT+GGG + chr3_2:78734398-78734417 Msa0428000:CDS 30.0%
TCAACAACAACAAAATTGTG+GGG + chr3_2:78734399-78734418 Msa0428000:CDS 30.0%
! TTGAGAGTATGAATAAGCAA+AGG - chr3_2:78734451-78734470 None:intergenic 30.0%
!! TTCATCGTTTTTATTTCGGA+TGG - chr3_2:78734609-78734628 None:intergenic 30.0%
!! ACAGCATTTTATTTGCTTTG+TGG + chr3_2:78734660-78734679 Msa0428000:CDS 30.0%
!! TTTGTGTTCCACTTTTGTTT+GGG - chr3_2:78734873-78734892 None:intergenic 30.0%
!! TTTTGTGTTCCACTTTTGTT+TGG - chr3_2:78734874-78734893 None:intergenic 30.0%
!! ATTCAGGATCAAGTTCTGAA+GGG - chr3_2:78732362-78732381 None:intergenic 35.0%
! TATTCAGGATCAAGTTCTGA+AGG - chr3_2:78732363-78732382 None:intergenic 35.0%
TAATGTGTACCTGTTCGTTT+AGG - chr3_2:78732405-78732424 None:intergenic 35.0%
GTTATGAGTCAGGAACAATA+TGG + chr3_2:78732678-78732697 Msa0428000:intron 35.0%
ATGAAGTATACAAAGTGACG+AGG - chr3_2:78732817-78732836 None:intergenic 35.0%
! TTCAAACATAATTTGCAGGC+GGG + chr3_2:78733195-78733214 Msa0428000:intron 35.0%
TATCTTTATGGCAGTGCAAT+AGG + chr3_2:78733254-78733273 Msa0428000:CDS 35.0%
TCAACTCACATCATGCTAAT+GGG - chr3_2:78733296-78733315 None:intergenic 35.0%
ATCAACTCACATCATGCTAA+TGG - chr3_2:78733297-78733316 None:intergenic 35.0%
!! TTGTTCTAAAGACCCACTAA+TGG - chr3_2:78733502-78733521 None:intergenic 35.0%
TCTAGATGTCTCCATTTATC+AGG + chr3_2:78733847-78733866 Msa0428000:CDS 35.0%
! GATCTTTTCCTCCTGATAAA+TGG - chr3_2:78733861-78733880 None:intergenic 35.0%
! AACCCATACATGATCAGTTT+TGG + chr3_2:78733898-78733917 Msa0428000:CDS 35.0%
CTCCAAAACTGATCATGTAT+GGG - chr3_2:78733903-78733922 None:intergenic 35.0%
ACTCCAAAACTGATCATGTA+TGG - chr3_2:78733904-78733923 None:intergenic 35.0%
TTCCAGAGTTTCATAATACG+TGG + chr3_2:78733947-78733966 Msa0428000:CDS 35.0%
! AGAGAACCAAGTTCTACTTT+TGG + chr3_2:78734173-78734192 Msa0428000:intron 35.0%
!!! GTGTTAACAGTCTCGTTTTT+TGG - chr3_2:78734263-78734282 None:intergenic 35.0%
AACAGAAAATGGTACCTTCA+AGG + chr3_2:78734344-78734363 Msa0428000:intron 35.0%
ACAGAAAATGGTACCTTCAA+GGG + chr3_2:78734345-78734364 Msa0428000:intron 35.0%
!! ATCGTTTTTATTTCGGATGG+TGG - chr3_2:78734606-78734625 None:intergenic 35.0%
TTAGTATGTACAATCCAGCT+TGG + chr3_2:78734739-78734758 Msa0428000:CDS 35.0%
! AGACAGGTTGAACATATACT+TGG - chr3_2:78734815-78734834 None:intergenic 35.0%
!! TTGTGTTCCACTTTTGTTTG+GGG - chr3_2:78734872-78734891 None:intergenic 35.0%
!! GTGTCGATCTTTTGTCTTCT+TGG + chr3_2:78732550-78732569 Msa0428000:intron 40.0%
CTGAAAAGGATCATCACCAA+AGG - chr3_2:78732794-78732813 None:intergenic 40.0%
CTAGCTGAATGTTATCGTTG+TGG + chr3_2:78732842-78732861 Msa0428000:CDS 40.0%
CTATTCCACAAACCGTGTAT+TGG - chr3_2:78733223-78733242 None:intergenic 40.0%
AGATGTCTCCATTTATCAGG+AGG + chr3_2:78733850-78733869 Msa0428000:CDS 40.0%
CACCACGTATTATGAAACTC+TGG - chr3_2:78733952-78733971 None:intergenic 40.0%
CAGAGTTTCATAATACGTGG+TGG + chr3_2:78733950-78733969 Msa0428000:CDS 40.0%
!! TCTTTTGGCTATTCCACAGT+TGG + chr3_2:78733997-78734016 Msa0428000:CDS 40.0%
TTAGCAATTTCCCAAACAGC+AGG + chr3_2:78734027-78734046 Msa0428000:CDS 40.0%
!!! ACAGTCTCGTTTTTTGGGAA+AGG - chr3_2:78734257-78734276 None:intergenic 40.0%
!!! TGGGGAATTTTTCAAGCTCT+TGG + chr3_2:78734417-78734436 Msa0428000:CDS 40.0%
TTATTTGCTTTGTGGCTGCA+TGG + chr3_2:78734668-78734687 Msa0428000:CDS 40.0%
TATTTGCTTTGTGGCTGCAT+GGG + chr3_2:78734669-78734688 Msa0428000:CDS 40.0%
GGAACACAAAATTCCAATCC+CGG + chr3_2:78734883-78734902 Msa0428000:CDS 40.0%
GAACACAAAATTCCAATCCC+GGG + chr3_2:78734884-78734903 Msa0428000:CDS 40.0%
AGATTGTAAGGACGAAAACC+CGG - chr3_2:78734905-78734924 None:intergenic 40.0%
AAGGGACACCATCTATGGAA+TGG - chr3_2:78732344-78732363 None:intergenic 45.0%
! TTCTGAAGGGACACCATCTA+TGG - chr3_2:78732349-78732368 None:intergenic 45.0%
ATAACAGCTGTGATTGGGTC+TGG + chr3_2:78732719-78732738 Msa0428000:CDS 45.0%
TAACAGCTGTGATTGGGTCT+GGG + chr3_2:78732720-78732739 Msa0428000:CDS 45.0%
ACAAAGTGACGAGGCTGAAA+AGG - chr3_2:78732808-78732827 None:intergenic 45.0%
TAGCTTCTCTTTCCATGCAC+AGG - chr3_2:78732872-78732891 None:intergenic 45.0%
GATGCAGTTCGTAGCATTCT+TGG + chr3_2:78732902-78732921 Msa0428000:CDS 45.0%
TGCTAAAAAGACGCCAACTG+TGG - chr3_2:78734013-78734032 None:intergenic 45.0%
! ACGACGATAACCTGCTGTTT+GGG - chr3_2:78734040-78734059 None:intergenic 45.0%
AACGACGATAACCTGCTGTT+TGG - chr3_2:78734041-78734060 None:intergenic 45.0%
CATTTGGAGAACAAGCACCA+GGG + chr3_2:78734700-78734719 Msa0428000:CDS 45.0%
ATACTTGGTATGCTCCCACA+AGG - chr3_2:78734800-78734819 None:intergenic 45.0%
TTCTCTACGAAGGCAAAGAC+AGG - chr3_2:78734831-78734850 None:intergenic 45.0%
GTCTTTGCCTTCGTAGAGAA+AGG + chr3_2:78734831-78734850 Msa0428000:CDS 45.0%
GAGAAAGGAGCAGCAAAAGA+TGG + chr3_2:78734846-78734865 Msa0428000:CDS 45.0%
AAGATGGCCCCAAACAAAAG+TGG + chr3_2:78734862-78734881 Msa0428000:CDS 45.0%
GATTGTAAGGACGAAAACCC+GGG - chr3_2:78734904-78734923 None:intergenic 45.0%
!! AAAAAAAAAAAAATTAATTC+GGG + chr3_2:78734288-78734307 Msa0428000:intron 5.0%
AACCGGAGCCATTCCATAGA+TGG + chr3_2:78732333-78732352 Msa0428000:CDS 50.0%
CACCATCTATGGAATGGCTC+CGG - chr3_2:78732338-78732357 None:intergenic 50.0%
! CCATCGTCGTCGAAGTATTC+AGG - chr3_2:78732378-78732397 None:intergenic 50.0%
CCTGAATACTTCGACGACGA+TGG + chr3_2:78732375-78732394 Msa0428000:CDS 50.0%
GATGGTCGTCCTAAACGAAC+AGG + chr3_2:78732393-78732412 Msa0428000:CDS 50.0%
AACAGCTGTGATTGGGTCTG+GGG + chr3_2:78732721-78732740 Msa0428000:CDS 50.0%
! TGGGGTGCTATCCTTAGCAT+GGG + chr3_2:78732739-78732758 Msa0428000:CDS 50.0%
! GCTATCCTTAGCATGGGCTA+CGG + chr3_2:78732745-78732764 Msa0428000:CDS 50.0%
!! ACGGCACAATTGGGTTGGAT+TGG + chr3_2:78732764-78732783 Msa0428000:CDS 50.0%
!! GCACAATTGGGTTGGATTGG+TGG + chr3_2:78732767-78732786 Msa0428000:CDS 50.0%
GGGTTGCTTGACATGTGACA+TGG - chr3_2:78733883-78733902 None:intergenic 50.0%
ACGGTAACTCGAACGAACTC+AGG + chr3_2:78734223-78734242 Msa0428000:intron 50.0%
!! ACCTTCAAGGGTACTCTCAC+TGG + chr3_2:78734357-78734376 Msa0428000:CDS 50.0%
!! TCCAGTGAGAGTACCCTTGA+AGG - chr3_2:78734361-78734380 None:intergenic 50.0%
!! TTCAAGGGTACTCTCACTGG+AGG + chr3_2:78734360-78734379 Msa0428000:CDS 50.0%
! TGCATGGGCTATGCTGCATT+TGG + chr3_2:78734684-78734703 Msa0428000:CDS 50.0%
GCATTTGGAGAACAAGCACC+AGG + chr3_2:78734699-78734718 Msa0428000:CDS 50.0%
AATCCAGTGAGCAAGTTCCC+TGG - chr3_2:78734720-78734739 None:intergenic 50.0%
! TCCAGCTTGGCTCATCGATT+TGG + chr3_2:78734752-78734771 Msa0428000:CDS 50.0%
!! TCCCACAAGGTGGATTACGA+CGG - chr3_2:78734787-78734806 None:intergenic 50.0%
TGCTGCTCCTTTCTCTACGA+AGG - chr3_2:78734841-78734860 None:intergenic 50.0%
TAAGGACGAAAACCCGGGAT+TGG - chr3_2:78734899-78734918 None:intergenic 50.0%
! CTGGGGTGCTATCCTTAGCA+TGG + chr3_2:78732738-78732757 Msa0428000:CDS 55.0%
TTGTGCCGTAGCCCATGCTA+AGG - chr3_2:78732753-78732772 None:intergenic 55.0%
! AGCATGGGCTACGGCACAAT+TGG + chr3_2:78732754-78732773 Msa0428000:CDS 55.0%
! GCATGGGCTACGGCACAATT+GGG + chr3_2:78732755-78732774 Msa0428000:CDS 55.0%
!! GGGTTGGATTGGTGGTCCTT+TGG + chr3_2:78732775-78732794 Msa0428000:CDS 55.0%
CGTTGTGGAGATCCTGTGCA+TGG + chr3_2:78732857-78732876 Msa0428000:CDS 55.0%
GCCAAATCGATGAGCCAAGC+TGG - chr3_2:78734756-78734775 None:intergenic 55.0%
TGCCGTCGTAATCCACCTTG+TGG + chr3_2:78734782-78734801 Msa0428000:CDS 55.0%
GCCGTCGTAATCCACCTTGT+GGG + chr3_2:78734783-78734802 Msa0428000:CDS 55.0%
CTTGGTATGCTCCCACAAGG+TGG - chr3_2:78734797-78734816 None:intergenic 55.0%
GGGCTACGGCACAATTGGGT+TGG + chr3_2:78732759-78732778 Msa0428000:CDS 60.0%
GGGCGCCAATACACGGTTTG+TGG + chr3_2:78733215-78733234 Msa0428000:CDS 60.0%
! GCACCAGGGAACTTGCTCAC+TGG + chr3_2:78734714-78734733 Msa0428000:CDS 60.0%
TGCAGGCGGGCGCCAATACA+CGG + chr3_2:78733208-78733227 Msa0428000:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 78732309 78734926 78732309 ID=Msa0428000;Name=Msa0428000
chr3_2 mRNA 78732309 78734926 78732309 ID=Msa0428000-mRNA-1;Parent=Msa0428000;Name=Msa0428000-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|6|0|375
chr3_2 exon 78732309 78732414 78732309 ID=Msa0428000-mRNA-1:exon:20941;Parent=Msa0428000-mRNA-1
chr3_2 exon 78732690 78732923 78732690 ID=Msa0428000-mRNA-1:exon:20942;Parent=Msa0428000-mRNA-1
chr3_2 exon 78733213 78733306 78733213 ID=Msa0428000-mRNA-1:exon:20943;Parent=Msa0428000-mRNA-1
chr3_2 exon 78733834 78734048 78733834 ID=Msa0428000-mRNA-1:exon:20944;Parent=Msa0428000-mRNA-1
chr3_2 exon 78734349 78734488 78734349 ID=Msa0428000-mRNA-1:exon:20945;Parent=Msa0428000-mRNA-1
chr3_2 exon 78734588 78734926 78734588 ID=Msa0428000-mRNA-1:exon:20946;Parent=Msa0428000-mRNA-1
chr3_2 CDS 78732309 78732414 78732309 ID=Msa0428000-mRNA-1:cds;Parent=Msa0428000-mRNA-1
chr3_2 CDS 78732690 78732923 78732690 ID=Msa0428000-mRNA-1:cds;Parent=Msa0428000-mRNA-1
chr3_2 CDS 78733213 78733306 78733213 ID=Msa0428000-mRNA-1:cds;Parent=Msa0428000-mRNA-1
chr3_2 CDS 78733834 78734048 78733834 ID=Msa0428000-mRNA-1:cds;Parent=Msa0428000-mRNA-1
chr3_2 CDS 78734349 78734488 78734349 ID=Msa0428000-mRNA-1:cds;Parent=Msa0428000-mRNA-1
chr3_2 CDS 78734588 78734926 78734588 ID=Msa0428000-mRNA-1:cds;Parent=Msa0428000-mRNA-1
Gene Sequence

>Msa0428000

ATGTTGAAAGAAGAAATAGAAAGAAACCGGAGCCATTCCATAGATGGTGTCCCTTCAGAACTTGATCCTGAATACTTCGACGACGATGGTCGTCCTAAACGAACAGGAACAATATGGACAACAAGTTCTCATATAATAACAGCTGTGATTGGGTCTGGGGTGCTATCCTTAGCATGGGCTACGGCACAATTGGGTTGGATTGGTGGTCCTTTGGTGATGATCCTTTTCAGCCTCGTCACTTTGTATACTTCATCAATGCTAGCTGAATGTTATCGTTGTGGAGATCCTGTGCATGGAAAGAGAAGCTATACTTTTGTGGATGCAGTTCGTAGCATTCTTGGCGGGCGCCAATACACGGTTTGTGGAATAGTTCAGTACTTGTATCTTTATGGCAGTGCAATAGGATACTCGATTGCAGCTCCCATTAGCATGATGGAAATAAACAAGTCTAGATGTCTCCATTTATCAGGAGGAAAAGATCCATGTCACATGTCAAGCAACCCATACATGATCAGTTTTGGAGTAATCGAAATTTTCGTTTCTCAAATTCCAGAGTTTCATAATACGTGGTGGCTCTCAGTAATAGCTGCAATAATGTCTTTTGGCTATTCCACAGTTGGCGTCTTTTTAGCAATTTCCCAAACAGCAGAAAATGGTACCTTCAAGGGTACTCTCACTGGAGGAAGCACTGAAGATGTGTCAACAACAACAAAATTGTGGGGAATTTTTCAAGCTCTTGGTGACATAGCCTTTGCTTATTCATACTCTCAAATTCTCATTGAAATTCAGGACACTATAAAAAGTCCACCATCCGAAATAAAAACGATGAAGAATGCAGCAGCTTTAAGTGTTGCTGTCACTACAGCATTTTATTTGCTTTGTGGCTGCATGGGCTATGCTGCATTTGGAGAACAAGCACCAGGGAACTTGCTCACTGGATTTAGTATGTACAATCCAGCTTGGCTCATCGATTTGGCGAATGCTGCCGTCGTAATCCACCTTGTGGGAGCATACCAAGTATATGTTCAACCTGTCTTTGCCTTCGTAGAGAAAGGAGCAGCAAAAGATGGCCCCAAACAAAAGTGGAACACAAAATTCCAATCCCGGGTTTTCGTCCTTACAATCTAA
Protein sequence

>Msa0428000

MLKEEIERNRSHSIDGVPSELDPEYFDDDGRPKRTGTIWTTSSHIITAVIGSGVLSLAWATAQLGWIGGPLVMILFSLVTLYTSSMLAECYRCGDPVHGKRSYTFVDAVRSILGGRQYTVCGIVQYLYLYGSAIGYSIAAPISMMEINKSRCLHLSGGKDPCHMSSNPYMISFGVIEIFVSQIPEFHNTWWLSVIAAIMSFGYSTVGVFLAISQTAENGTFKGTLTGGSTEDVSTTTKLWGIFQALGDIAFAYSYSQILIEIQDTIKSPPSEIKTMKNAAALSVAVTTAFYLLCGCMGYAAFGEQAPGNLLTGFSMYNPAWLIDLANAAVVIHLVGAYQVYVQPVFAFVEKGAAKDGPKQKWNTKFQSRVFVLTI*