AlfalfaGEDB | Msa1382700

M. sativa cultivar zhongmu-4 / Msa1382700

KEGG: -
GO: GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015172 GO:0015175 GO:0015318 GO:0015399 GO:0015711 GO:0015800 GO:0015804 GO:0015849 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039
PFAM: PF01490.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1382700	XP_003602666.1	95.556	90	4	0	1	90	388	477	9.14e-51	176

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1382700	sp\|Q8GUM3\|AAP5_ARATH	60.000	90	36	0	1	90	391	480	4.81e-36	129

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1382700	G7JC68	95.556	90	4	0	1	90	388	477	4.36e-51	176

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016570	Msa1382700	0.809022	2.323643e-50	-8.615850e-47
Msa0102070	Msa1382700	0.847695	9.826013e-60	-8.615850e-47
Msa1382690	Msa1382700	0.841850	3.700588e-58	-8.615850e-47
Msa1382700	Msa1397580	0.804220	2.398712e-49	-8.615850e-47
Msa1382700	Msa1404000	0.826776	2.253227e-54	-8.615850e-47
Msa1382700	Msa1415310	0.827163	1.821259e-54	-8.615850e-47
Msa1382700	Msa1422180	0.831021	2.114197e-55	-8.615850e-47
Msa1382700	Msa1433120	0.837659	4.561144e-57	-8.615850e-47
Msa1382700	Msa1451570	0.805834	1.102178e-49	-8.615850e-47
Msa0636170	Msa1382700	0.808434	3.102441e-50	-8.615850e-47
Msa0639610	Msa1382700	0.806450	8.178468e-50	-8.615850e-47
Msa0678930	Msa1382700	0.809547	1.793207e-50	-8.615850e-47
Msa0694370	Msa1382700	0.809422	1.907558e-50	-8.615850e-47
Msa0726140	Msa1382700	0.838307	3.108163e-57	-8.615850e-47
Msa0729800	Msa1382700	0.823534	1.315575e-53	-8.615850e-47
Msa0743810	Msa1382700	0.842025	3.326170e-58	-8.615850e-47
Msa0745060	Msa1382700	0.834076	3.693398e-56	-8.615850e-47
Msa0767720	Msa1382700	0.812482	4.145878e-51	-8.615850e-47
Msa0783160	Msa1382700	0.862467	4.964479e-64	-8.615850e-47
Msa0808010	Msa1382700	0.830297	3.179430e-55	-8.615850e-47
Msa0808020	Msa1382700	0.830032	3.689653e-55	-8.615850e-47
Msa0811080	Msa1382700	0.803805	2.925204e-49	-8.615850e-47
Msa0825860	Msa1382700	0.842702	2.200684e-58	-8.615850e-47
Msa0142220	Msa1382700	0.811103	8.275522e-51	-8.615850e-47
Msa0153910	Msa1382700	0.823022	1.731943e-53	-8.615850e-47
Msa0227480	Msa1382700	0.820134	8.051331e-53	-8.615850e-47
Msa0232850	Msa1382700	0.831858	1.315441e-55	-8.615850e-47
Msa1269670	Msa1382700	0.834947	2.232412e-56	-8.615850e-47
Msa1270230	Msa1382700	0.846423	2.192347e-59	-8.615850e-47
Msa1270240	Msa1382700	0.829929	3.908817e-55	-8.615850e-47
Msa1270270	Msa1382700	0.817310	3.523401e-52	-8.615850e-47
Msa1283330	Msa1382700	0.844689	6.471014e-59	-8.615850e-47
Msa1285930	Msa1382700	0.865964	4.029316e-65	-8.615850e-47
Msa1287800	Msa1382700	0.833442	5.321071e-56	-8.615850e-47
Msa1287810	Msa1382700	0.851412	9.028624e-61	-8.615850e-47
Msa1340590	Msa1382700	0.812951	3.273524e-51	-8.615850e-47
Msa0378360	Msa1382700	0.880999	3.453309e-70	-8.615850e-47
Msa0384800	Msa1382700	0.809282	2.043578e-50	-8.615850e-47
Msa0414410	Msa1382700	0.815763	7.823944e-52	-8.615850e-47
Msa0414420	Msa1382700	0.857833	1.246519e-62	-8.615850e-47
Msa0427710	Msa1382700	0.878286	3.176539e-69	-8.615850e-47
Msa0428010	Msa1382700	0.868964	4.413698e-66	-8.615850e-47
Msa0436780	Msa1382700	0.804273	2.338383e-49	-8.615850e-47
Msa0450000	Msa1382700	0.818313	2.092263e-52	-8.615850e-47
Msa0450570	Msa1382700	0.814452	1.529903e-51	-8.615850e-47
Msa0452900	Msa1382700	0.859441	4.127029e-63	-8.615850e-47
Msa0453120	Msa1382700	0.823689	1.209812e-53	-8.615850e-47
Msa0981370	Msa1382700	0.823323	1.473590e-53	-8.615850e-47
Msa0989060	Msa1382700	0.803619	3.196680e-49	-8.615850e-47
Msa1016280	Msa1382700	0.830359	3.070443e-55	-8.615850e-47
Msa1025360	Msa1382700	0.832209	1.077281e-55	-8.615850e-47
Msa1025370	Msa1382700	0.820029	8.510884e-53	-8.615850e-47
Msa1044830	Msa1382700	0.825333	4.962785e-54	-8.615850e-47
Msa1044840	Msa1382700	0.836604	8.489404e-57	-8.615850e-47
Msa1092990	Msa1382700	0.846601	1.960215e-59	-8.615850e-47
Msa1100980	Msa1382700	0.861809	7.905104e-64	-8.615850e-47
Msa0267000	Msa1382700	0.821497	3.913605e-53	-8.615850e-47
Msa0267010	Msa1382700	0.829452	5.108634e-55	-8.615850e-47
Msa0270520	Msa1382700	0.811717	6.088595e-51	-8.615850e-47
Msa0306460	Msa1382700	0.803253	3.808308e-49	-8.615850e-47
Msa0342240	Msa1382700	0.833253	5.930833e-56	-8.615850e-47
Msa0360510	Msa1382700	0.847654	1.008622e-59	-8.615850e-47
Msa1109440	Msa1382700	0.831804	1.356101e-55	-8.615850e-47
Msa1121200	Msa1382700	0.844576	6.941322e-59	-8.615850e-47
Msa1135740	Msa1382700	0.852228	5.297555e-61	-8.615850e-47
Msa1136090	Msa1382700	0.808499	3.005702e-50	-8.615850e-47
Msa1146110	Msa1382700	0.824697	7.012552e-54	-8.615850e-47
Msa1187940	Msa1382700	0.833965	3.938559e-56	-8.615850e-47
Msa1187950	Msa1382700	0.863657	2.128387e-64	-8.615850e-47
Msa1192640	Msa1382700	0.848073	7.730802e-60	-8.615850e-47
Msa0491590	Msa1382700	0.810998	8.723065e-51	-8.615850e-47
Msa0506270	Msa1382700	0.804210	2.409543e-49	-8.615850e-47
Msa0518540	Msa1382700	0.887078	1.957015e-72	-8.615850e-47
Msa0846210	Msa1382700	0.816842	4.489999e-52	-8.615850e-47
Msa0846330	Msa1382700	0.801622	8.254062e-49	-8.615850e-47
Msa0846350	Msa1382700	0.816963	4.216989e-52	-8.615850e-47
Msa0865320	Msa1382700	0.830585	2.703574e-55	-8.615850e-47
Msa0872230	Msa1382700	0.802375	5.780493e-49	-8.615850e-47
Msa0885290	Msa1382700	0.826888	2.117938e-54	-8.615850e-47
Msa0888370	Msa1382700	0.812226	4.716093e-51	-8.615850e-47
Msa0956270	Msa1382700	0.801487	8.796548e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1382700	MtrunA17_Chr3g0130641	95.556	90	4	0	1	90	388	477	8.40e-55	176
Msa1382700	MtrunA17_Chr3g0125081	66.667	90	30	0	1	90	390	479	4.83e-40	137
Msa1382700	MtrunA17_Chr3g0130661	68.966	87	27	0	1	87	92	178	9.46e-40	129
Msa1382700	MtrunA17_Chr3g0125101	65.556	90	31	0	1	90	391	480	7.67e-39	134
Msa1382700	MtrunA17_Chr3g0125091	64.444	90	32	0	1	90	391	480	1.52e-37	130
Msa1382700	MtrunA17_Chr8g0384661	62.500	88	33	0	3	90	407	494	2.00e-36	127
Msa1382700	MtrunA17_Chr4g0037201	58.889	90	37	0	1	90	423	512	1.55e-35	125
Msa1382700	MtrunA17_Chr1g0159761	58.889	90	37	0	1	90	386	475	1.34e-30	112
Msa1382700	MtrunA17_Chr1g0206821	56.180	89	39	0	1	89	442	530	1.08e-29	109
Msa1382700	MtrunA17_Chr1g0206851	52.874	87	41	0	1	87	292	378	1.77e-29	107
Msa1382700	MtrunA17_Chr3g0130651	73.563	87	23	0	1	87	379	465	1.89e-28	105
Msa1382700	MtrunA17_Chr3g0140761	47.191	89	47	0	1	89	393	481	9.84e-27	101
Msa1382700	MtrunA17_Chr1g0206871	49.438	89	45	0	1	89	382	470	1.74e-26	100
Msa1382700	MtrunA17_Chr1g0146491	48.315	89	46	0	1	89	383	471	7.69e-26	99.0
Msa1382700	MtrunA17_Chr1g0206831	54.321	81	37	0	1	81	255	335	9.49e-26	97.4
Msa1382700	MtrunA17_Chr5g0447221	54.430	79	36	0	1	79	385	463	2.53e-25	97.4
Msa1382700	MtrunA17_Chr3g0140531	47.674	86	45	0	1	86	382	467	1.30e-24	95.5
Msa1382700	MtrunA17_Chr3g0103251	51.899	79	38	0	1	79	292	370	6.39e-24	92.8
Msa1382700	MtrunA17_Chr3g0103231	51.899	79	38	0	1	79	379	457	7.79e-24	93.2
Msa1382700	MtrunA17_Chr3g0140541	47.500	80	42	0	7	86	387	466	6.51e-23	90.9
Msa1382700	MtrunA17_Chr3g0103211	48.101	79	41	0	1	79	379	457	1.31e-22	89.7
Msa1382700	MtrunA17_Chr3g0103221	49.367	79	40	0	1	79	386	464	1.38e-22	89.7
Msa1382700	MtrunA17_Chr1g0146391	45.977	87	47	0	1	87	368	454	1.94e-21	86.7
Msa1382700	MtrunA17_Chr1g0146331	42.529	87	50	0	1	87	377	463	3.32e-21	85.9
Msa1382700	MtrunA17_Chr2g0293331	41.379	87	51	0	1	87	368	454	7.28e-20	82.0
Msa1382700	MtrunA17_Chr1g0146411	39.286	84	51	0	3	86	386	469	2.96e-19	80.5
Msa1382700	MtrunA17_Chr3g0103271	46.667	75	40	0	4	78	387	461	1.01e-18	79.0
Msa1382700	MtrunA17_Chr5g0447231	34.211	76	50	0	4	79	385	460	1.55e-15	70.1
Msa1382700	MtrunA17_Chr1g0184721	34.444	90	55	1	1	86	348	437	8.59e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1382700	AT1G44100.1	60.000	90	36	1	90	391	480	4.89e-37	129
Msa1382700	AT5G09220.1	61.111	90	35	1	90	404	493	9.26e-37	129
Msa1382700	AT5G63850.1	57.778	90	38	1	90	377	466	6.12e-35	124
Msa1382700	AT1G77380.1	62.222	90	34	1	90	387	476	4.27e-33	119
Msa1382700	AT5G49630.1	56.818	88	38	1	88	393	480	7.26e-30	110
Msa1382700	AT1G58360.1	53.333	90	42	1	90	395	484	1.95e-29	109
Msa1382700	AT1G10010.1	53.409	88	41	1	88	386	473	5.33e-29	107
Msa1382700	AT1G10010.2	53.409	88	41	1	88	387	474	5.42e-29	107
Msa1382700	AT5G23810.1	55.172	58	26	1	58	386	443	8.88e-17	73.9

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCATTTAGAACTCCATTT+TGG	0.108522	tig0020524:-4320	None:intergenic
TCCTTTCTTTAATGATGTTC+TGG	0.137749	tig0020524:+4220	Msa1382700:CDS
TTCTTGGAGCAGTGGGATTT+TGG	0.180772	tig0020524:+4246	Msa1382700:CDS
AATGATGTTCTGGGATTTCT+TGG	0.212555	tig0020524:+4230	Msa1382700:CDS
ACTGGATAATAAACTGTTAA+AGG	0.224075	tig0020524:-4269	None:intergenic
CCTTTCTTTAATGATGTTCT+GGG	0.306108	tig0020524:+4221	Msa1382700:CDS
TCCCAAAATGGAGTTCTAAA+TGG	0.320619	tig0020524:+4318	Msa1382700:CDS
TGAATGGCTTGTAATGCTTT+AGG	0.330057	tig0020524:-4424	None:intergenic
GTATCAGTTGTGGCTGCTCT+TGG	0.389248	tig0020524:+4377	Msa1382700:CDS
CTTTAGGTCCTCTATAATAC+TGG	0.396377	tig0020524:-4408	None:intergenic
CTGGGATTTCTTGGAGCAGT+GGG	0.412618	tig0020524:+4239	Msa1382700:CDS
TGCAAAGGAAGATCCCAAAA+TGG	0.417168	tig0020524:+4306	Msa1382700:CDS
ACATTGCAACAAAAGTAGTT+AGG	0.463138	tig0020524:-4193	None:intergenic
CCCAGAACATCATTAAAGAA+AGG	0.503059	tig0020524:-4221	None:intergenic
CTCAACCGCCAGTATTATAG+AGG	0.511484	tig0020524:+4400	Msa1382700:CDS
ATGCTTTATTGTATCAGTTG+TGG	0.512651	tig0020524:+4367	Msa1382700:CDS
GCTCAATACTCTGAGCTGAA+TGG	0.555896	tig0020524:-4440	None:intergenic
TCTGGGATTTCTTGGAGCAG+TGG	0.566045	tig0020524:+4238	Msa1382700:CDS
TTTAACAGTTTATTATCCAG+TGG	0.568524	tig0020524:+4271	Msa1382700:CDS
TGCAAGATATACATCTCCAC+TGG	0.575990	tig0020524:-4287	None:intergenic
TGGAGATGTATATCTTGCAA+AGG	0.629124	tig0020524:+4291	Msa1382700:CDS
TAGGTCCTCTATAATACTGG+CGG	0.635831	tig0020524:-4405	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTGGATAATAAACTGTTAA+AGG	-	tig0020524:4272-4291	None:intergenic	25.0%
!	TTTAACAGTTTATTATCCAG+TGG	+	tig0020524:4271-4290	Msa1382700:CDS	25.0%
	ACATTGCAACAAAAGTAGTT+AGG	-	tig0020524:4196-4215	None:intergenic	30.0%
	TCCTTTCTTTAATGATGTTC+TGG	+	tig0020524:4220-4239	Msa1382700:CDS	30.0%
	CCTTTCTTTAATGATGTTCT+GGG	+	tig0020524:4221-4240	Msa1382700:CDS	30.0%
!!	TCCATTTAGAACTCCATTTT+GGG	-	tig0020524:4322-4341	None:intergenic	30.0%
!!	ATCCATTTAGAACTCCATTT+TGG	-	tig0020524:4323-4342	None:intergenic	30.0%
!!!	GAGTTCTAAATGGATTTTGT+TGG	+	tig0020524:4328-4347	Msa1382700:CDS	30.0%
!	ATGCTTTATTGTATCAGTTG+TGG	+	tig0020524:4367-4386	Msa1382700:CDS	30.0%
	CCCAGAACATCATTAAAGAA+AGG	-	tig0020524:4224-4243	None:intergenic	35.0%
!!	AATGATGTTCTGGGATTTCT+TGG	+	tig0020524:4230-4249	Msa1382700:CDS	35.0%
	TGGAGATGTATATCTTGCAA+AGG	+	tig0020524:4291-4310	Msa1382700:CDS	35.0%
!!	TCCCAAAATGGAGTTCTAAA+TGG	+	tig0020524:4318-4337	Msa1382700:CDS	35.0%
	CTTTAGGTCCTCTATAATAC+TGG	-	tig0020524:4411-4430	None:intergenic	35.0%
!	TGAATGGCTTGTAATGCTTT+AGG	-	tig0020524:4427-4446	None:intergenic	35.0%
	TGCAAGATATACATCTCCAC+TGG	-	tig0020524:4290-4309	None:intergenic	40.0%
	TGCAAAGGAAGATCCCAAAA+TGG	+	tig0020524:4306-4325	Msa1382700:CDS	40.0%
	TAGGTCCTCTATAATACTGG+CGG	-	tig0020524:4408-4427	None:intergenic	40.0%
!	TTCTTGGAGCAGTGGGATTT+TGG	+	tig0020524:4246-4265	Msa1382700:CDS	45.0%
	CTCAACCGCCAGTATTATAG+AGG	+	tig0020524:4400-4419	Msa1382700:CDS	45.0%
!	TCTGGGATTTCTTGGAGCAG+TGG	+	tig0020524:4238-4257	Msa1382700:CDS	50.0%
	CTGGGATTTCTTGGAGCAGT+GGG	+	tig0020524:4239-4258	Msa1382700:CDS	50.0%
	GTATCAGTTGTGGCTGCTCT+TGG	+	tig0020524:4377-4396	Msa1382700:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
tig0020524	gene	4188	4460	4188	ID=Msa1382700;Name=Msa1382700
tig0020524	mRNA	4188	4460	4188	ID=Msa1382700-mRNA-1;Parent=Msa1382700;Name=Msa1382700-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|90
tig0020524	exon	4188	4460	4188	ID=Msa1382700-mRNA-1:exon:8307;Parent=Msa1382700-mRNA-1
tig0020524	CDS	4188	4460	4188	ID=Msa1382700-mRNA-1:cds;Parent=Msa1382700-mRNA-1

Gene Sequence	>Msa1382700 ATGATCCTAACTACTTTTGTTGCAATGTTGATTCCTTTCTTTAATGATGTTCTGGGATTTCTTGGAGCAGTGGGATTTTGGCCTTTAACAGTTTATTATCCAGTGGAGATGTATATCTTGCAAAGGAAGATCCCAAAATGGAGTTCTAAATGGATTTTGTTGGAAATCATAAGTGTTATATGCTTTATTGTATCAGTTGTGGCTGCTCTTGGCTCAACCGCCAGTATTATAGAGGACCTAAAGCATTACAAGCCATTCAGCTCAGAGTATTGA
Protein sequence	>Msa1382700 MILTTFVAMLIPFFNDVLGFLGAVGFWPLTVYYPVEMYILQRKIPKWSSKWILLEIISVICFIVSVVAALGSTASIIEDLKHYKPFSSEY*