AlfalfaGEDB | Msa0544220

M. sativa cultivar zhongmu-4 / Msa0544220

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544220	GAU31888.1	78.000	50	9	1	8	55	5	54	2.06e-14	83.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544220	A0A445HN61	66.038	53	16	2	5	55	5	57	3.57e-14	84.3

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0077410	Msa0544220	0.825337	4.953121e-54	-8.615850e-47
Msa0125280	Msa0544220	0.815914	7.240689e-52	-8.615850e-47
Msa0143080	Msa0544220	0.800042	1.735374e-48	-8.615850e-47
Msa0277530	Msa0544220	0.804094	2.547641e-49	-8.615850e-47
Msa0361250	Msa0544220	0.817780	2.761652e-52	-8.615850e-47
Msa0361260	Msa0544220	0.843790	1.128305e-58	-8.615850e-47
Msa0408640	Msa0544220	0.854441	1.228659e-61	-8.615850e-47
Msa0464630	Msa0544220	0.808911	2.453662e-50	-8.615850e-47
Msa0510440	Msa0544220	0.810537	1.097076e-50	-8.615850e-47
Msa0519200	Msa0544220	0.801487	8.798929e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 222 sgRNAs with CRISPR-Local

Find 612 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCTTGACCAAGAAAGGTT+TGG	0.435048	4_1:+32393283	Msa0544220:CDS
AATGGTGGCTCAGGGCACGG+GGG	0.576479	4_1:+32394111	Msa0544220:CDS
ACCACTACCAGAACCAATGC+CGG	0.556664	4_1:-32394388	None:intergenic
ACCGGCATTGGTTCTGGTAG+TGG	0.455957	4_1:+32394387	Msa0544220:CDS
AGACGTGGACCTCGCTGTAG+AGG	0.582247	4_1:+32393325	Msa0544220:CDS
AGAGGTTGGGGTGGTCGCCG+TGG	0.593292	4_1:+32393343	Msa0544220:CDS
AGCGAGGTCCACGTCTAAAT+CGG	0.399523	4_1:-32393318	None:intergenic
AGGAATTGGTATTGGAATTG+GGG	0.448544	4_1:+32394329	Msa0544220:CDS
AGTGGAAGTGGAGCAGGTAT+AGG	0.358426	4_1:+32394273	Msa0544220:CDS
AGTGGAGCAGGTATAGGTGG+TGG	0.585692	4_1:+32394279	Msa0544220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATCTTAGTATGTTCACTCT+TGG	+	chr4_1:32393253-32393272	Msa0544220:CDS	35.0%
TATCATCTCCAAACCTTTCT+TGG	-	chr4_1:32393294-32393313	None:intergenic	35.0%
ATTGGCATAGGAATTGGTAT+TGG	+	chr4_1:32394321-32394340	Msa0544220:CDS	35.0%
GATCTTAGTATGTTCACTCT+TGG	+	chr4_1:32393253-32393272	Msa0544220:CDS	35.0%
TATCATCTCCAAACCTTTCT+TGG	-	chr4_1:32393294-32393313	None:intergenic	35.0%
ATTGGCATAGGAATTGGTAT+TGG	+	chr4_1:32394321-32394340	Msa0544220:CDS	35.0%
ATCACAAGCTTGACCAAGAA+AGG	+	chr4_1:32393278-32393297	Msa0544220:CDS	40.0%
AAGCTTGACCAAGAAAGGTT+TGG	+	chr4_1:32393283-32393302	Msa0544220:CDS	40.0%
ATCACAAGCTTGACCAAGAA+AGG	+	chr4_1:32393278-32393297	Msa0544220:CDS	40.0%
AAGCTTGACCAAGAAAGGTT+TGG	+	chr4_1:32393283-32393302	Msa0544220:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	32393124	32394434	32393124	ID=Msa0544220;Name=Msa0544220
chr4_1	mRNA	32393124	32394434	32393124	ID=Msa0544220-mRNA-1;Parent=Msa0544220;Name=Msa0544220-mRNA-1;_AED=0.36;_eAED=0.50;_QI=63\|0\|0.5\|1\|0\|0\|2\|0\|348
chr4_1	exon	32393124	32393382	32393124	ID=Msa0544220-mRNA-1:exon:8302;Parent=Msa0544220-mRNA-1
chr4_1	exon	32393584	32394434	32393584	ID=Msa0544220-mRNA-1:exon:8303;Parent=Msa0544220-mRNA-1
chr4_1	five_prime_UTR	32393124	32393186	32393124	ID=Msa0544220-mRNA-1:five_prime_utr;Parent=Msa0544220-mRNA-1
chr4_1	CDS	32393187	32393382	32393187	ID=Msa0544220-mRNA-1:cds;Parent=Msa0544220-mRNA-1
chr4_1	CDS	32393584	32394434	32393584	ID=Msa0544220-mRNA-1:cds;Parent=Msa0544220-mRNA-1

Gene Sequence

>Msa0544220

ATGGCATTTATTGGTTTCCACGGGAAATGGCTTTCTTTGAGTGTTCTTGTTCTCTTCATTGTTCTTGATCTTAGTATGTTCACTCTTGGTGATCACAAGCTTGACCAAGAAAGGTTTGGAGATGATAATTGCCGATTTAGACGTGGACCTCGCTGTAGAGGTTGGGGTGGTCGCCGTGGTGGGGGTGGCTTTGGAGGTGGTGGAGCTGGAGGAGGCCTTGGTGGTGGTGGTGGAGGAGGAAGTGGTGGTGGGGGTGGATTAGGCGGTGGCTCAGGACAAGGTGGAGGTTTTGGTGCTGGTGGAGGTGTAGGAGGTGGAGCTGGTGGTGGTGGAGGTTTAGGTGGTGGTGGTGGAGGAGGTAGTGGAGGTGGAGGTGGAGTAGGTGGCGGTTCAGGACACGGTGGAGGATTTGGTGCTGGAGGAGGTGTAGGTGGTGGAGCTGGAGGAGGCATTGGTGGTGGTGGAGGTGGCGGTAGTGGCGGTGGAGGTGGAGTGGGTGGTGGCTCAGGACAGGGTGGAGGATTTGGTGCTGGTGGAGGTGTAGGTGGTGGAGCTGGAGGGGGCATTGGTGGTGGTGGAGGAGGAGGTAGTGGTGGTGGAGGTGGAGTAGGCGGTGGTTCAGGACATGGTGAAGGATTTGGTGCTGGTGGAGGTGTAGGTGGTGGAGCTGGAGGAGGCATTGCTGGTGGTGGCGGTGGAGGAGGAGGTGGTGGTGGTGGCTCGAATGGTGGCTCAGGGCACGGGGGAGGGTTTGGAGCTGGTGTTGGCGGAGGTGCAGGAGGTGTCGGTGGAGGAGGTGGTGGAGGCGGTGGCGGTGGAGGTGGTGGAGGAGCAGGCGGAGGTTCAGGACATGGTGGAGGCTTTGGTGCAGGTGGTGGTGTAGGAAGTGGAAGTGGAGCAGGTATAGGTGGTGGTCGTGGAGGTGGAGGAGGAATTGGCATAGGAATTGGTATTGGAATTGGGGTTGGTGTAGGAGCTGGAGCTGGCCAAGGATCTGGTACCGGCATTGGTTCTGGTAGTGGTGGAGGAGGTGGTGCTAAACATTAA

Protein sequence

>Msa0544220

MAFIGFHGKWLSLSVLVLFIVLDLSMFTLGDHKLDQERFGDDNCRFRRGPRCRGWGGRRGGGGFGGGGAGGGLGGGGGGGSGGGGGLGGGSGQGGGFGAGGGVGGGAGGGGGLGGGGGGGSGGGGGVGGGSGHGGGFGAGGGVGGGAGGGIGGGGGGGSGGGGGVGGGSGQGGGFGAGGGVGGGAGGGIGGGGGGGSGGGGGVGGGSGHGEGFGAGGGVGGGAGGGIAGGGGGGGGGGGGSNGGSGHGGGFGAGVGGGAGGVGGGGGGGGGGGGGGGAGGGSGHGGGFGAGGGVGSGSGAGIGGGRGGGGGIGIGIGIGIGVGVGAGAGQGSGTGIGSGSGGGGGAKH*