AlfalfaGEDB | Msa1365020

M. sativa cultivar zhongmu-4 / Msa1365020

KEGG: -
GO: -
PFAM: PF01190.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365020	XP_003604031.1	89.286	196	7	2	1	196	1	182	1.05e-107	321

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365020	G7J8H3	89.286	196	7	2	1	196	1	182	5.03e-108	321

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa1365020	0.824112	9.631045e-54	-8.615850e-47
Msa0017120	Msa1365020	0.821998	2.996122e-53	-8.615850e-47
Msa0065390	Msa1365020	0.810178	1.311463e-50	-8.615850e-47
Msa0077410	Msa1365020	0.849037	4.180726e-60	-8.615850e-47
Msa0094420	Msa1365020	0.800894	1.163607e-48	-8.615850e-47
Msa0100570	Msa1365020	0.803952	2.726490e-49	-8.615850e-47
Msa0110710	Msa1365020	0.812876	3.399534e-51	-8.615850e-47
Msa0125280	Msa1365020	0.822565	2.212804e-53	-8.615850e-47
Msa0134130	Msa1365020	0.802374	5.782418e-49	-8.615850e-47
Msa0135980	Msa1365020	0.816396	5.649641e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365020	MtrunA17_Chr3g0145511	89.286	196	7	2	1	196	1	182	9.69e-112	321

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365020	AT2G16630.1	46.067	178	75	2	7	164	9	185	1.31e-50	167

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Find 63 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATTGGTGGTAATGG+TGG	0.570401	tig0018197:+67179	None:intergenic
AAAATCCAAGACTCATGTTT+AGG	0.352973	tig0018197:-67367	Msa1365020:CDS
AACATCCTAAACATGAGTCT+TGG	0.489630	tig0018197:+67362	None:intergenic
AAGGCAGATACTATGCTCAC+CGG	0.644788	tig0018197:-68884	Msa1365020:CDS
AATGGTGGAAGTTGTGGCAT+TGG	0.390842	tig0018197:+67194	None:intergenic
AATGTTAGAAGAAGTCCAGT+TGG	0.611637	tig0018197:+68974	None:intergenic
AATGTTGGTGGTGGTGGGGA+TGG	0.300659	tig0018197:+67260	None:intergenic
AGCTATACAATGAGGTTTGA+TGG	0.433754	tig0018197:-67485	Msa1365020:CDS
AGTGGCCATTGATGGAACAA+AGG	0.622178	tig0018197:-68903	Msa1365020:CDS
AGTTAGATCTCACCATTAAC+AGG	0.396284	tig0018197:+68809	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTGTGATCAATGCAAAGA+CGG	-	tig0018197:67366-67385	Msa1365020:CDS	30.0%
GAGTTGAATGACAAAATGTA+TGG	+	tig0018197:67499-67518	None:intergenic	30.0%
ATGCAGGAGAAAAACTAAAA+TGG	+	tig0018197:67555-67574	None:intergenic	30.0%
ACTATGATAGAGGTTCAATT+TGG	+	tig0018197:67630-67649	None:intergenic	30.0%
ATTGAACCTCTATCATAGTT+TGG	-	tig0018197:67631-67650	Msa1365020:intron	30.0%
CAAATGGCAAGAACTATTAA+CGG	+	tig0018197:67677-67696	None:intergenic	30.0%
GAGTTTATGAACTATCTTAC+GGG	+	tig0018197:67819-67838	None:intergenic	30.0%
AACTTGTGCCTTAAAAATAC+AGG	+	tig0018197:68020-68039	None:intergenic	30.0%
TATGATAACTTATGCCCTAA+AGG	+	tig0018197:68162-68181	None:intergenic	30.0%
TCAAAAAGAAGTTCAACAGT+TGG	-	tig0018197:68342-68361	Msa1365020:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0018197	gene	67148	69094	67148	ID=Msa1365020;Name=Msa1365020
tig0018197	mRNA	67148	69094	67148	ID=Msa1365020-mRNA-1;Parent=Msa1365020;Name=Msa1365020-mRNA-1;_AED=0.38;_eAED=0.38;_QI=122\|1\|0.5\|1\|1\|1\|2\|0\|196
tig0018197	exon	67148	67587	67148	ID=Msa1365020-mRNA-1:exon:14358;Parent=Msa1365020-mRNA-1
tig0018197	exon	68822	69094	68822	ID=Msa1365020-mRNA-1:exon:14357;Parent=Msa1365020-mRNA-1
tig0018197	five_prime_UTR	68973	69094	68973	ID=Msa1365020-mRNA-1:five_prime_utr;Parent=Msa1365020-mRNA-1
tig0018197	CDS	68822	68972	68822	ID=Msa1365020-mRNA-1:cds;Parent=Msa1365020-mRNA-1
tig0018197	CDS	67148	67587	67148	ID=Msa1365020-mRNA-1:cds;Parent=Msa1365020-mRNA-1

Gene Sequence

>Msa1365020

ATGGAGTTGCCGGAGAAGCTTTTGTTTGCAACATTTTTAATTCTTTCAGTGGCCATTGATGGAACAAAGGCAGATACTATGCTCACCGGCACTGTCATTTGTGATCAATGCAAAGACGGGCAGAGATCTCTTTTCGATTATCCTGTTAATGGTGCAAAAGTAACTCTGTCATGTAGTGACAACAATGGGCAAGTAACAATGTCAAGAGAAGAAACAACAAATTGGTTTGGAAGCTATACAATGAGGTTTGATGGAGCACCAGACTTAGGTGGTTGTTCTGCACATGTCTCGGGAAATAATAATGGACAAGGAGGACCAATGAGTTGTGGTGAAGGTGCTGGTCCTGCTCAAAATCCAAGACTCATGTTTAGGATGTTTGACATGGAGATGTATGCTGTGGATCCACTCCTTGCTCAGCCTGCTCAACCTATGCAATACTGCTCAACATCTTCCAACCCATCCCCACCACCACCAACATTGGCACCACCCATTTCTTCTCCTCCTCCACATTTCAACCTACCCCCAATGCCACAACTTCCACCATTACCACCAATGATTTTTCCAGAAGCTTCAGCATGTCCATCACAGTAA

Protein sequence

>Msa1365020

MELPEKLLFATFLILSVAIDGTKADTMLTGTVICDQCKDGQRSLFDYPVNGAKVTLSCSDNNGQVTMSREETTNWFGSYTMRFDGAPDLGGCSAHVSGNNNGQGGPMSCGEGAGPAQNPRLMFRMFDMEMYAVDPLLAQPAQPMQYCSTSSNPSPPPPTLAPPISSPPPHFNLPPMPQLPPLPPMIFPEASACPSQ*