AlfalfaGEDB | Msa0615740

M. sativa cultivar zhongmu-4 / Msa0615740

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615740	KAF1881981.1	56.748	615	189	8	5	586	2	572	0.0	663

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615740	sp\|Q9LKR4\|FRS10_ARATH	44.444	297	146	3	31	317	27	314	3.78e-76	258
Msa0615740	sp\|Q9LKR4\|FRS10_ARATH	39.130	184	111	1	318	500	491	674	1.50e-34	142

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615740	A0A6A5N3Q2	56.748	615	189	8	5	586	2	572	0.0	663

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TFs/TRs:

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Gene ID	Type	Classification
Msa0615740	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0013050	Msa0615740	-0.804121	2.514312e-49	-8.615850e-47
Msa0028700	Msa0615740	0.845508	3.886500e-59	-8.615850e-47
Msa0032330	Msa0615740	0.855126	7.776076e-62	-8.615850e-47
Msa0178960	Msa0615740	0.827594	1.435428e-54	-8.615850e-47
Msa0178970	Msa0615740	0.816506	5.339440e-52	-8.615850e-47
Msa0197660	Msa0615740	0.804983	1.662180e-49	-8.615850e-47
Msa0239800	Msa0615740	0.809699	1.662687e-50	-8.615850e-47
Msa0278110	Msa0615740	0.801136	1.038291e-48	-8.615850e-47
Msa0317290	Msa0615740	0.840193	1.007668e-57	-8.615850e-47
Msa0317510	Msa0615740	0.803279	3.760497e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615740	MtrunA17_Chr5g0431001	88.013	317	38	0	1	317	1	317	0.0	583
Msa0615740	MtrunA17_Chr1g0198761	71.528	288	81	1	31	317	16	303	8.86e-142	429
Msa0615740	MtrunA17_Chr1g0198761	72.662	278	76	0	315	592	479	756	2.89e-141	427
Msa0615740	MtrunA17_Chr2g0276841	44.961	258	137	2	60	317	69	321	2.75e-73	248
Msa0615740	MtrunA17_Chr2g0276841	40.625	192	108	3	315	503	497	685	2.11e-39	153
Msa0615740	MtrunA17_Chr2g0294841	45.977	261	133	3	60	317	44	299	4.71e-68	235
Msa0615740	MtrunA17_Chr2g0294841	32.130	277	160	5	315	569	475	745	1.30e-38	151
Msa0615740	MtrunA17_Chr2g0290391	44.030	268	131	3	61	317	51	310	7.70e-68	233
Msa0615740	MtrunA17_Chr2g0290391	44.776	201	91	5	315	505	486	676	3.90e-38	149
Msa0615740	MtrunA17_Chr5g0430921	96.471	85	3	0	502	586	1	85	1.80e-51	171

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0615740	AT5G28530.3	44.444	297	146	3	31	317	27	314	3.23e-77	258
Msa0615740	AT5G28530.3	41.667	168	97	1	334	500	503	670	7.14e-35	140
Msa0615740	AT5G28530.2	44.444	297	146	3	31	317	27	314	3.23e-77	258
Msa0615740	AT5G28530.2	41.667	168	97	1	334	500	503	670	7.14e-35	140
Msa0615740	AT5G28530.1	44.444	297	146	3	31	317	27	314	3.84e-77	258
Msa0615740	AT5G28530.1	39.130	184	111	1	318	500	491	674	1.52e-35	142
Msa0615740	AT1G10240.2	47.101	276	134	5	48	317	30	299	3.80e-73	247
Msa0615740	AT1G10240.2	39.320	206	123	2	315	519	475	679	9.97e-41	157
Msa0615740	AT1G10240.1	47.101	276	134	5	48	317	30	299	3.80e-73	247
Msa0615740	AT1G10240.1	39.320	206	123	2	315	519	475	679	9.97e-41	157

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Find 101 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAAGGTCGGCCAAA+AGG	0.495104	4_2:-65276403	Msa0615740:CDS
AAAGACAAGTGAACGAGAAC+AGG	0.453486	4_2:-65276557	Msa0615740:CDS
AAAGGATCAAGACCAAAAGG+AGG	0.655085	4_2:-65276385	Msa0615740:CDS
AACCATTATGAGATGCCTCT+TGG	0.446043	4_2:-65277472	Msa0615740:CDS
AACGTCACAGTCAGCATTGT+TGG	0.377007	4_2:+65276444	None:intergenic
AAGGATCAAGACCAAAAGGA+GGG	0.611165	4_2:-65276384	Msa0615740:CDS
AAGTCAATAACATTGATGAG+GGG	0.714581	4_2:-65278230	Msa0615740:CDS
AAGTCACTAACATTGATGGA+TGG	0.540573	4_2:-65278338	Msa0615740:CDS
AAGTTTGAAAGATCGAGATG+AGG	0.682349	4_2:-65277623	Msa0615740:CDS
AATATCATCATAAAGATAAA+AGG	0.310779	4_2:-65276514	Msa0615740:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAATATTTGCCCTTCAAA+AGG	+	chr4_2:65275978-65275997	None:intergenic	30.0%
TCAAGTCAATAACATTGATG+AGG	-	chr4_2:65276055-65276074	Msa0615740:three_prime_UTR	30.0%
CAAGTCAATAACATTGATGA+GGG	-	chr4_2:65276056-65276075	Msa0615740:three_prime_UTR	30.0%
AAGTCAATAACATTGATGAG+GGG	-	chr4_2:65276057-65276076	Msa0615740:three_prime_UTR	30.0%
GTAGTTACTAAGAGAAGAAT+TGG	-	chr4_2:65276341-65276360	Msa0615740:CDS	30.0%
CAATTGTGAATTCATATTGG+AGG	+	chr4_2:65276691-65276710	None:intergenic	30.0%
ACAAATTGGAGCATATTGTT+TGG	-	chr4_2:65276720-65276739	Msa0615740:CDS	30.0%
CAAATTGGAGCATATTGTTT+GGG	-	chr4_2:65276721-65276740	Msa0615740:CDS	30.0%
AAAGTCCTTCAAATAAGCTA+AGG	+	chr4_2:65277039-65277058	None:intergenic	30.0%
GTCAAAATTAGAAATCAAGC+TGG	-	chr4_2:65277248-65277267	Msa0615740:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	65275903	65278406	65275903	ID=Msa0615740;Name=Msa0615740
chr4_2	mRNA	65275903	65278406	65275903	ID=Msa0615740-mRNA-1;Parent=Msa0615740;Name=Msa0615740-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0.5\|0.66\|1\|1\|1\|3\|248\|592
chr4_2	exon	65275903	65276172	65275903	ID=Msa0615740-mRNA-1:exon:18449;Parent=Msa0615740-mRNA-1
chr4_2	exon	65276259	65277073	65276259	ID=Msa0615740-mRNA-1:exon:18448;Parent=Msa0615740-mRNA-1
chr4_2	exon	65277465	65278406	65277465	ID=Msa0615740-mRNA-1:exon:18447;Parent=Msa0615740-mRNA-1
chr4_2	CDS	65277465	65278406	65277465	ID=Msa0615740-mRNA-1:cds;Parent=Msa0615740-mRNA-1
chr4_2	CDS	65276259	65277073	65276259	ID=Msa0615740-mRNA-1:cds;Parent=Msa0615740-mRNA-1
chr4_2	CDS	65276151	65276172	65276151	ID=Msa0615740-mRNA-1:cds;Parent=Msa0615740-mRNA-1
chr4_2	three_prime_UTR	65275903	65276150	65275903	ID=Msa0615740-mRNA-1:three_prime_utr;Parent=Msa0615740-mRNA-1

Gene Sequence

>Msa0615740

ATGATATCTGAAGTAAGCAATGATGCTAGTGATTGGCCCTTGCCTCAAGTCACTAACATTGATGGATGGGTTCCTTTTGAAGGGCAAATATTTGAAGATGATGAATATGTAAATTTTTTAACATTTCAAGAGCTACAACATGAACCCTTGCCTCAAGTCAATAACATTGATGAGGGGATTCCTTTTGAAGGGAAAATATTTGAAAGTGATAATGCTGCTTTTGAATTTTATTGTTTATTTGCAAAACAAAATGGATTTTCTATTAGGCGTGATCACATTTATAAATCTTGTAAATTTGTGTCTGAAGAGAATCCATCGGGTGTTTATAAGAGGGAATTTGTTTGTCATCGTGGTGGTATTGGTAAACCACGCAAAACTGTTGAAGTGGAAAATCAAAGGAAACGAAAATCTTCAAGATGTGATTGTAGTGCAAAGATGGTAGTTACTAAGAGAAGAATTGGCATTGAGGAAAAATGGGTGGTTAAACATTTCAACAATTCTCACAACCACGAGTTATTGGATGACAAAGAGGTTCAATTTCTTCCTGCTTATCGTAACATTCCAATTGTTGATCAAGATCGCATACTATTATTGTCTAAAGCCGGTTGCCCTGTAAGTCTTATAGCGAGAGTGCTTGAGTTAGAGAAAAGAACAGGCACAGGAAATTTACCATTCTTAGAAAAAGATATTAGAAATTTTATTCAATCATATAGTGGTATTGGTAAAGAAAGTGATGTTTCAGATGTTCTCAGATTGTGCAAAAGTTTGAAAGATCGAGATGAGGCCCTCCAATATGAATTCACAATTGATGGAAGTAACAAATTGGAGCATATTGTTTGGGCATTTGGTGATTCAATTCGTGCATATGAAGCTTTTGGAGATGTTGTAGTTTTTGATACCACATATAGAATAAACCATTATGAGATGCCTCTTGGAATATGGGTTGGTGTTGCTGTCAAAATTAGAAATCAAGCTGGAGAAGAGGCACGAATGCGTCAAAAGTATCATAATCCTCAGATTACAACACATTTTCCTATTGAAGAACATGCCTCGTCTATACTCACACCTTATGCCTTTAAGTTGCTTCAACATGAGATCGAATTATCTGCAAAGTATGCAGCAATTGAAGCTGCCAATGGCTCTTATGTTGTGCAACATCATACCAAAGTTGATGGTGGTCGTATAGTAAGTTTGATAAAAGAAGAAGAGTCAATTCATTGTTCTTGTAAAGAGTTTCAATTTTCAGGAATAATATGTAGACATGCAATTCGAGTGCTAATGTTGAAAAATTATTTTGTTCTTCCATCAAAATATCTTCCTTTTCGATGGAGACGAGAAAGTTCATTGATCCCAAAATCTAGTCATGTAATAAACTGCAATGATGGATCCTCTGCTAAATTTCAGTCTCTAATTCGATGTTTGGAAGTCGAATCATTAAAGACAAGTGAACGAGAACAGGTTGCCAAAAGAGAGTTAGAAAATATCATCATAAAGATAAAAGGCATGCCTGAAACTCAAGAACATGTGATTGATTTGGAAGAGGATGTTTCCAACAATGCTGACTGTGACGTTGAAAATCCTGTTGTTTCAAAAACAAAAGGTCGGCCAAAAGGATCAAGACCAAAAGGAGGGGTTGAAGCTGCCAAGAAGCCTCGTCGTTGTCATGTTCCAGATTGTGGTGGAACTAATCACGACTCACGAAACTGTCCAAACAAAAAGAGAAAAATGGAAGTGTTGCCTTCTCAATCTCCTAATAAGATCATGAAGGGTTGGAAGTAG

Protein sequence

>Msa0615740

MISEVSNDASDWPLPQVTNIDGWVPFEGQIFEDDEYVNFLTFQELQHEPLPQVNNIDEGIPFEGKIFESDNAAFEFYCLFAKQNGFSIRRDHIYKSCKFVSEENPSGVYKREFVCHRGGIGKPRKTVEVENQRKRKSSRCDCSAKMVVTKRRIGIEEKWVVKHFNNSHNHELLDDKEVQFLPAYRNIPIVDQDRILLLSKAGCPVSLIARVLELEKRTGTGNLPFLEKDIRNFIQSYSGIGKESDVSDVLRLCKSLKDRDEALQYEFTIDGSNKLEHIVWAFGDSIRAYEAFGDVVVFDTTYRINHYEMPLGIWVGVAVKIRNQAGEEARMRQKYHNPQITTHFPIEEHASSILTPYAFKLLQHEIELSAKYAAIEAANGSYVVQHHTKVDGGRIVSLIKEEESIHCSCKEFQFSGIICRHAIRVLMLKNYFVLPSKYLPFRWRRESSLIPKSSHVINCNDGSSAKFQSLIRCLEVESLKTSEREQVAKRELENIIIKIKGMPETQEHVIDLEEDVSNNADCDVENPVVSKTKGRPKGSRPKGGVEAAKKPRRCHVPDCGGTNHDSRNCPNKKRKMEVLPSQSPNKIMKGWK*