AlfalfaGEDB | Msa0616540

M. sativa cultivar zhongmu-4 / Msa0616540

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0616540	XP_039685416.1	97.235	217	6	0	1	217	1	217	7.96e-155	445

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0616540	sp\|Q9CAC3\|CRK43_ARATH	65.899	217	71	2	1	217	1	214	9.88e-100	298

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0616540	A0A396GUS2	97.235	217	6	0	1	217	1	217	3.80e-155	445

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0178860	Msa0616540	-0.819505	1.121267e-52	-8.615850e-47
Msa0223380	Msa0616540	0.805672	1.192368e-49	-8.615850e-47
Msa0279210	Msa0616540	-0.811062	8.446659e-51	-8.615850e-47
Msa0279250	Msa0616540	-0.814021	1.904279e-51	-8.615850e-47
Msa0318970	Msa0616540	-0.813385	2.628802e-51	-8.615850e-47
Msa0389810	Msa0616540	0.819105	1.383248e-52	-8.615850e-47
Msa0464900	Msa0616540	0.818632	1.771762e-52	-8.615850e-47
Msa0468130	Msa0616540	0.817669	2.924926e-52	-8.615850e-47
Msa0473240	Msa0616540	0.800403	1.465158e-48	-8.615850e-47
Msa0482190	Msa0616540	-0.803626	3.187129e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0616540	MtrunA17_Chr8g0376131	97.235	217	6	0	1	217	1	217	7.32e-159	445
Msa0616540	MtrunA17_Chr1g0160921	70.046	217	64	1	1	217	1	216	4.85e-113	329
Msa0616540	MtrunA17_Chr8g0358161	58.011	181	74	2	38	217	33	212	2.10e-70	218
Msa0616540	MtrunA17_Chr8g0354421	57.216	194	81	2	25	217	337	529	3.58e-70	226
Msa0616540	MtrunA17_Chr6g0484231	58.333	180	71	2	39	217	38	214	8.29e-70	218
Msa0616540	MtrunA17_Chr7g0236531	51.220	205	97	3	14	217	2	204	1.54e-69	216
Msa0616540	MtrunA17_Chr1g0154331	56.216	185	79	2	34	217	22	205	2.37e-69	216
Msa0616540	MtrunA17_Chr7g0236161	55.612	196	83	3	24	217	443	636	5.07e-69	224
Msa0616540	MtrunA17_Chr7g0236201	55.612	196	83	3	24	217	443	636	6.20e-69	224
Msa0616540	MtrunA17_Chr7g0236121	55.612	196	83	3	24	217	477	670	9.14e-69	224

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0616540	AT1G70740.1	65.899	217	71	2	1	217	13	226	1.16e-100	298
Msa0616540	AT1G70740.2	65.899	217	71	2	1	217	1	214	1.41e-100	298
Msa0616540	AT4G23180.3	59.777	179	70	2	40	217	336	513	1.46e-70	224
Msa0616540	AT4G11530.1	61.453	179	67	2	40	217	340	517	2.35e-70	227
Msa0616540	AT4G23180.2	60.452	177	68	2	42	217	338	513	9.38e-70	223
Msa0616540	AT4G23180.1	59.777	179	70	2	40	217	336	513	2.31e-69	224
Msa0616540	AT4G23270.2	58.659	179	72	2	40	217	314	491	1.24e-67	219
Msa0616540	AT4G23270.1	58.659	179	72	2	40	217	314	491	1.24e-67	219
Msa0616540	AT4G23200.2	59.887	177	69	2	42	217	326	501	1.78e-67	215
Msa0616540	AT4G23270.3	58.659	179	72	2	40	217	314	491	2.01e-67	219

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Find 133 sgRNAs with CRISPR-Local

Find 701 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTGATGTGTTTAGCTA+TGG	0.504382	4_2:-66425977	Msa0616540:three_prime_UTR
AAATTGAAGGATTATAGTAT+AGG	0.423918	4_2:+66434627	None:intergenic
AACAGCTTGATTGGAAACGT+AGG	0.660997	4_2:-66432636	Msa0616540:CDS
AACCTAACATTTCACGGATT+AGG	0.425586	4_2:+66426627	None:intergenic
AACTTGTCTAGGCTTTCATG+AGG	0.633257	4_2:+66433969	None:intergenic
AAGAATGTGGTGAATTTGTT+GGG	0.479229	4_2:-66434038	Msa0616540:CDS
AAGAGGGAAACGTCCAATGC+TGG	0.481255	4_2:-66425172	Msa0616540:three_prime_UTR
AAGCCAAGTCAAAACACGTG+TGG	0.673987	4_2:-66432449	Msa0616540:CDS
AAGCTGATGTGTTTAGCTAT+GGG	0.467302	4_2:-66425976	Msa0616540:three_prime_UTR
AAGTTAGGCGAGGGTGGTTT+TGG	0.290654	4_2:-66434300	Msa0616540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATGAGGTTGTATGTTGTT+TGG	+	chr4_2:66425013-66425032	None:intergenic	30.0%
GAATACTGTGAAGAAAACTA+TGG	+	chr4_2:66425222-66425241	None:intergenic	30.0%
ATTCTCAAACACTTCAAATG+CGG	-	chr4_2:66425238-66425257	Msa0616540:three_prime_UTR	30.0%
AGTAGCATTAAAATTCTTGG+TGG	+	chr4_2:66425439-66425458	None:intergenic	30.0%
TTATCACAGACATCAAATCA+AGG	-	chr4_2:66425652-66425671	Msa0616540:intron	30.0%
TATCACAGACATCAAATCAA+GGG	-	chr4_2:66425653-66425672	Msa0616540:intron	30.0%
TATGAATGAGGCAAAATTGT+TGG	-	chr4_2:66425687-66425706	Msa0616540:intron	30.0%
TAAGAATGTGGTGAATTTGT+TGG	-	chr4_2:66425723-66425742	Msa0616540:intron	30.0%
TACTGAATAGAAACTTGTCT+AGG	+	chr4_2:66425807-66425826	None:intergenic	30.0%
AGCAATGCACTACTAAATTA+TGG	+	chr4_2:66426088-66426107	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	66424961	66434823	66424961	ID=Msa0616540;Name=Msa0616540
chr4_2	mRNA	66424961	66434823	66424961	ID=Msa0616540-mRNA-1;Parent=Msa0616540;Name=Msa0616540-mRNA-1;_AED=0.09;_eAED=0.09;_QI=247\|1\|1\|1\|0.66\|0.57\|7\|1487\|218
chr4_2	exon	66424961	66425571	66424961	ID=Msa0616540-mRNA-1:exon:18932;Parent=Msa0616540-mRNA-1
chr4_2	exon	66425895	66426045	66425895	ID=Msa0616540-mRNA-1:exon:18931;Parent=Msa0616540-mRNA-1
chr4_2	exon	66426214	66426753	66426214	ID=Msa0616540-mRNA-1:exon:18930;Parent=Msa0616540-mRNA-1
chr4_2	exon	66432250	66432676	66432250	ID=Msa0616540-mRNA-1:exon:18929;Parent=Msa0616540-mRNA-1
chr4_2	exon	66433961	66434171	66433961	ID=Msa0616540-mRNA-1:exon:18928;Parent=Msa0616540-mRNA-1
chr4_2	exon	66434287	66434426	66434287	ID=Msa0616540-mRNA-1:exon:18927;Parent=Msa0616540-mRNA-1
chr4_2	exon	66434513	66434823	66434513	ID=Msa0616540-mRNA-1:exon:18926;Parent=Msa0616540-mRNA-1
chr4_2	five_prime_UTR	66434577	66434823	66434577	ID=Msa0616540-mRNA-1:five_prime_utr;Parent=Msa0616540-mRNA-1
chr4_2	CDS	66434513	66434576	66434513	ID=Msa0616540-mRNA-1:cds;Parent=Msa0616540-mRNA-1
chr4_2	CDS	66434287	66434426	66434287	ID=Msa0616540-mRNA-1:cds;Parent=Msa0616540-mRNA-1
chr4_2	CDS	66433961	66434171	66433961	ID=Msa0616540-mRNA-1:cds;Parent=Msa0616540-mRNA-1
chr4_2	CDS	66432435	66432676	66432435	ID=Msa0616540-mRNA-1:cds;Parent=Msa0616540-mRNA-1
chr4_2	three_prime_UTR	66432250	66432434	66432250	ID=Msa0616540-mRNA-1:three_prime_utr;Parent=Msa0616540-mRNA-1
chr4_2	three_prime_UTR	66426214	66426753	66426214	ID=Msa0616540-mRNA-1:three_prime_utr;Parent=Msa0616540-mRNA-1
chr4_2	three_prime_UTR	66425895	66426045	66425895	ID=Msa0616540-mRNA-1:three_prime_utr;Parent=Msa0616540-mRNA-1
chr4_2	three_prime_UTR	66424961	66425571	66424961	ID=Msa0616540-mRNA-1:three_prime_utr;Parent=Msa0616540-mRNA-1

Gene Sequence

>Msa0616540

ATGGACAAATCCCATAGTTTTCTTCACAGTATTCTCAAACACTTCAAATGCGGTTACAACCCAGAGGGAAACAATGAAGCTGAGTTACAAAGAATGGCTTCACGGGAGCAGAAGATATTTTCATATGAAACCCTGCTTTCTGCCACCAAGAATTTTAATGCTACTCATAAGTTAGGCGAGGGTGGTTTTGGACCTGTCTACAAGGGGAAACTGAGTGATGGGAGAGAAGTTGCAGTGAAGAAGTTATCACAGACATCAAATCAAGGGAAGAAGGAGTTTATGAATGAGGCAAAATTGTTGGCTCGTGTACAGCATAAGAATGTGGTGAATTTGTTGGGTTATTGTGTTCATGGCACAGAGAAGATACTTGTTTACGAGTATGTTCCTCATGAAAGCCTAGACAAGTTTCTATTCAAAGAAGCTGAGAAGAGAGAACAGCTTGATTGGAAACGTAGGTTTGGAATAATCACAGGCGTAGCAAAGGGGTTACTTTATCTACACGAAGACTCACACAGTTGTATAATTCATCGGGACATCAAGGCCAGTAATATATTGCTTGATGACAAATGGACGGCTAAGATTGCAGATTTTGGAATGGCACGTCTCTTCCCTGAAGATCAAAGCCAAGTCAAAACACGTGTGGCTGGAACCAAGTAA

Protein sequence

>Msa0616540

MDKSHSFLHSILKHFKCGYNPEGNNEAELQRMASREQKIFSYETLLSATKNFNATHKLGEGGFGPVYKGKLSDGREVAVKKLSQTSNQGKKEFMNEAKLLARVQHKNVVNLLGYCVHGTEKILVYEYVPHESLDKFLFKEAEKREQLDWKRRFGIITGVAKGLLYLHEDSHSCIIHRDIKASNILLDDKWTAKIADFGMARLFPEDQSQVKTRVAGTK*