Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0619080 | KYP58465.1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 5 | 140 | 1.76e-92 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0619080 | sp|P68428|H32_WHEAT | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 5.15e-95 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0619080 | A0A1D1ZGP1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 1.97e-92 | 272 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0020540 | Msa0619080 | 0.808772 | 2.628153e-50 | -8.615850e-47 |
| Msa0075530 | Msa0619080 | 0.830900 | 2.263560e-55 | -8.615850e-47 |
| Msa0603980 | Msa0619080 | 0.846229 | 2.476580e-59 | -8.615850e-47 |
| Msa0606100 | Msa0619080 | 0.835784 | 1.371568e-56 | -8.615850e-47 |
| Msa0619080 | Msa0621710 | 0.805090 | 1.578882e-49 | -8.615850e-47 |
| Msa0619080 | Msa0654740 | 0.813013 | 3.172340e-51 | -8.615850e-47 |
| Msa0619080 | Msa0707260 | 0.810634 | 1.045556e-50 | -8.615850e-47 |
| Msa0619080 | Msa0818880 | 0.816444 | 5.513892e-52 | -8.615850e-47 |
| Msa0619080 | Msa0838750 | 0.813832 | 2.096763e-51 | -8.615850e-47 |
| Msa0619080 | Msa0866530 | 0.809852 | 1.541855e-50 | -8.615850e-47 |
| Msa0619080 | Msa0900760 | 0.827116 | 1.868833e-54 | -8.615850e-47 |
| Msa0619080 | Msa0909760 | 0.834676 | 2.611481e-56 | -8.615850e-47 |
| Msa0619080 | Msa1008680 | 0.812862 | 3.424419e-51 | -8.615850e-47 |
| Msa0619080 | Msa1033920 | 0.826154 | 3.169210e-54 | -8.615850e-47 |
| Msa0619080 | Msa1042880 | 0.815831 | 7.558629e-52 | -8.615850e-47 |
| Msa0619080 | Msa1116660 | 0.813336 | 2.695600e-51 | -8.615850e-47 |
| Msa0619080 | Msa1139050 | 0.801440 | 8.995892e-49 | -8.615850e-47 |
| Msa0619080 | Msa1162880 | 0.829179 | 5.949921e-55 | -8.615850e-47 |
| Msa0619080 | Msa1201540 | 0.804061 | 2.588157e-49 | -8.615850e-47 |
| Msa0619080 | Msa1215980 | 0.800626 | 1.319800e-48 | -8.615850e-47 |
| Msa0619080 | Msa1244240 | 0.801683 | 8.021161e-49 | -8.615850e-47 |
| Msa0619080 | Msa1263740 | 0.800286 | 1.547885e-48 | -8.615850e-47 |
| Msa0619080 | Msa1393560 | 0.808154 | 3.559550e-50 | -8.615850e-47 |
| Msa0619080 | Msa1426320 | 0.805143 | 1.538674e-49 | -8.615850e-47 |
| Msa0619080 | Msa1447170 | 0.803068 | 4.159235e-49 | -8.615850e-47 |
| Msa0140730 | Msa0619080 | 0.855572 | 5.766490e-62 | -8.615850e-47 |
| Msa0168050 | Msa0619080 | 0.810139 | 1.337401e-50 | -8.615850e-47 |
| Msa0364680 | Msa0619080 | 0.809802 | 1.580116e-50 | -8.615850e-47 |
| Msa0385890 | Msa0619080 | 0.853050 | 3.086095e-61 | -8.615850e-47 |
| Msa0293040 | Msa0619080 | 0.800970 | 1.122408e-48 | -8.615850e-47 |
| Msa0553750 | Msa0619080 | 0.829704 | 4.435064e-55 | -8.615850e-47 |
| Msa0561560 | Msa0619080 | 0.803087 | 4.120309e-49 | -8.615850e-47 |
| Msa0571620 | Msa0619080 | 0.802358 | 5.826769e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0619080 | MtrunA17_Chr5g0410931 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr5g0410951 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr8g0383851 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr8g0383781 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr8g0390361 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr2g0295191 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr7g0237661 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr4g0033041 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr7g0218971 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr4g0046301 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr8g0364801 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0619080 | MtrunA17_Chr4g0054161 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0619080 | MtrunA17_Chr8g0363741 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0619080 | MtrunA17_Chr1g0156021 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0619080 | MtrunA17_Chr4g0054151 | 97.059 | 136 | 4 | 0 | 1 | 136 | 4 | 139 | 2.61e-93 | 265 |
| Msa0619080 | MtrunA17_Chr8g0388121 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.09e-92 | 264 |
| Msa0619080 | MtrunA17_Chr2g0319141 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0619080 | MtrunA17_Chr2g0319151 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0619080 | MtrunA17_Chr2g0319131 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.38e-82 | 238 |
| Msa0619080 | MtrunA17_Chr2g0319121 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 5.82e-75 | 219 |
| Msa0619080 | MtrunA17_Chr7g0218961 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 8.01e-66 | 195 |
| Msa0619080 | MtrunA17_Chr7g0222991 | 67.164 | 134 | 43 | 1 | 1 | 134 | 10 | 142 | 5.47e-56 | 171 |
| Msa0619080 | MtrunA17_Chr5g0407311 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.29e-45 | 144 |
| Msa0619080 | MtrunA17_Chr7g0223001 | 58.647 | 133 | 41 | 3 | 3 | 134 | 27 | 146 | 5.46e-43 | 139 |
| Msa0619080 | MtrunA17_Chr7g0240791 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 9.61e-41 | 133 |
| Msa0619080 | MtrunA17_Chr8g0347231 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 3.80e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0619080 | AT1G09200.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0619080 | AT5G65360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0619080 | AT5G10390.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0619080 | AT5G10400.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0619080 | AT3G27360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0619080 | AT4G40030.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT4G40030.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT4G40030.4 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT5G10980.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT4G40040.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT4G40040.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT4G40040.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0619080 | AT4G40030.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 7.05e-93 | 266 |
| Msa0619080 | AT5G65350.1 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 5.30e-91 | 260 |
| Msa0619080 | AT1G75600.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.25e-89 | 256 |
| Msa0619080 | AT1G13370.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 5.55e-89 | 254 |
| Msa0619080 | AT1G19890.1 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.07e-83 | 241 |
| Msa0619080 | AT5G12910.1 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 9.90e-67 | 198 |
| Msa0619080 | AT1G01370.2 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0619080 | AT1G01370.1 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0619080 | AT1G01370.4 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0619080 | AT1G01370.3 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTGTGGCGAGCTGCTTC+CGG | 0.229871 | 4_2:-70118575 | None:intergenic |
| CTTTGTGGCGAGCTGCTTCC+GGG | 0.231058 | 4_2:-70118574 | None:intergenic |
| TCTCTCAAAGCAACTGTTCC+AGG | 0.333816 | 4_2:-70118654 | None:intergenic |
| ATTCCAGAGCAGTGCTGTTT+CGG | 0.340103 | 4_2:+70118776 | Msa0619080:CDS |
| AGAAGCGGCTGAAGCTTACT+TGG | 0.416086 | 4_2:+70118806 | Msa0619080:CDS |
| GAGCCGAAACAGCACTGCTC+TGG | 0.422418 | 4_2:-70118779 | None:intergenic |
| TTCTTCACTCCTCCGGTCGC+CGG | 0.442119 | 4_2:-70118615 | None:intergenic |
| GACGTTGGAATGGAAGTTTA+CGG | 0.458530 | 4_2:-70118713 | None:intergenic |
| AGGCATGATTGTAACTCTCT+TGG | 0.461766 | 4_2:-70118868 | None:intergenic |
| CTTGCAAGCTGAATGTCCTT+AGG | 0.462646 | 4_2:-70118888 | None:intergenic |
| CGAAAATCAGCTCCGGCGAC+CGG | 0.463917 | 4_2:+70118603 | Msa0619080:CDS |
| CCACCGGCGGCAAGGCTCCC+CGG | 0.480154 | 4_2:+70118556 | Msa0619080:CDS |
| GTGTGGCTTCTTCACTCCTC+CGG | 0.499925 | 4_2:-70118622 | None:intergenic |
| CAGTGCTCTTCTGATACTTA+CGG | 0.501233 | 4_2:-70118680 | None:intergenic |
| CTTCCGGGGAGCCTTGCCGC+CGG | 0.507682 | 4_2:-70118559 | None:intergenic |
| GGACTTACGAGCTGTTTGCT+TGG | 0.508768 | 4_2:-70118535 | None:intergenic |
| GGCCGCTCGAAAATCAGCTC+CGG | 0.532014 | 4_2:+70118596 | Msa0619080:CDS |
| CCGGGGAGCCTTGCCGCCGG+TGG | 0.548377 | 4_2:-70118556 | None:intergenic |
| TCGTAAGTCCACCGGCGGCA+AGG | 0.553533 | 4_2:+70118548 | Msa0619080:CDS |
| GCGGCTGAAGCTTACTTGGT+CGG | 0.565776 | 4_2:+70118810 | Msa0619080:CDS |
| GAGAGTTACAATCATGCCTA+AGG | 0.573275 | 4_2:+70118872 | Msa0619080:CDS |
| AAGAAGAATCAGAGGAGAGA+GGG | 0.573886 | 4_2:+70118908 | Msa0619080:CDS |
| TTTGTGGCGAGCTGCTTCCG+GGG | 0.576886 | 4_2:-70118573 | None:intergenic |
| TCACGAACAAGACGTTGGAA+TGG | 0.598115 | 4_2:-70118723 | None:intergenic |
| AAATCAGCTCCGGCGACCGG+AGG | 0.599225 | 4_2:+70118606 | Msa0619080:CDS |
| CAAGAAGAATCAGAGGAGAG+AGG | 0.603703 | 4_2:+70118907 | Msa0619080:CDS |
| CCGGAAGCAGCTCGCCACAA+AGG | 0.611340 | 4_2:+70118575 | Msa0619080:CDS |
| CGATTTCACGAACAAGACGT+TGG | 0.613448 | 4_2:-70118728 | None:intergenic |
| CAGCTTGCAAGAAGAATCAG+AGG | 0.621034 | 4_2:+70118900 | Msa0619080:CDS |
| CAAACAGCTCGTAAGTCCAC+CGG | 0.642395 | 4_2:+70118540 | Msa0619080:CDS |
| AAGCCACACAGATTCAGACC+TGG | 0.672238 | 4_2:+70118636 | Msa0619080:CDS |
| TGTTTCGGCTCTTCAAGAAG+CGG | 0.672573 | 4_2:+70118791 | Msa0619080:CDS |
| GTTCCAGGTCTGAATCTGTG+TGG | 0.688220 | 4_2:-70118639 | None:intergenic |
| ACAGCTCGTAAGTCCACCGG+CGG | 0.695900 | 4_2:+70118543 | Msa0619080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAGTGCTCTTCTGATACTTA+CGG | - | chr4_2:70118683-70118702 | None:intergenic | 40.0% | |
| GACGTTGGAATGGAAGTTTA+CGG | - | chr4_2:70118716-70118735 | None:intergenic | 40.0% | |
| AGGCATGATTGTAACTCTCT+TGG | - | chr4_2:70118871-70118890 | None:intergenic | 40.0% | |
| GAGAGTTACAATCATGCCTA+AGG | + | chr4_2:70118872-70118891 | Msa0619080:CDS | 40.0% | |
| AAGAAGAATCAGAGGAGAGA+GGG | + | chr4_2:70118908-70118927 | Msa0619080:CDS | 40.0% | |
| TCTCTCAAAGCAACTGTTCC+AGG | - | chr4_2:70118657-70118676 | None:intergenic | 45.0% | |
| TCACGAACAAGACGTTGGAA+TGG | - | chr4_2:70118726-70118745 | None:intergenic | 45.0% | |
| CGATTTCACGAACAAGACGT+TGG | - | chr4_2:70118731-70118750 | None:intergenic | 45.0% | |
| !! | ATTCCAGAGCAGTGCTGTTT+CGG | + | chr4_2:70118776-70118795 | Msa0619080:CDS | 45.0% |
| TGTTTCGGCTCTTCAAGAAG+CGG | + | chr4_2:70118791-70118810 | Msa0619080:CDS | 45.0% | |
| CTTGCAAGCTGAATGTCCTT+AGG | - | chr4_2:70118891-70118910 | None:intergenic | 45.0% | |
| CAGCTTGCAAGAAGAATCAG+AGG | + | chr4_2:70118900-70118919 | Msa0619080:CDS | 45.0% | |
| CAAGAAGAATCAGAGGAGAG+AGG | + | chr4_2:70118907-70118926 | Msa0619080:CDS | 45.0% | |
| GGACTTACGAGCTGTTTGCT+TGG | - | chr4_2:70118538-70118557 | None:intergenic | 50.0% | |
| CAAACAGCTCGTAAGTCCAC+CGG | + | chr4_2:70118540-70118559 | Msa0619080:CDS | 50.0% | |
| !! | TGATTTTCGAGCGGCCTTTG+TGG | - | chr4_2:70118592-70118611 | None:intergenic | 50.0% |
| AAGCCACACAGATTCAGACC+TGG | + | chr4_2:70118636-70118655 | Msa0619080:CDS | 50.0% | |
| GTTCCAGGTCTGAATCTGTG+TGG | - | chr4_2:70118642-70118661 | None:intergenic | 50.0% | |
| AGAAGCGGCTGAAGCTTACT+TGG | + | chr4_2:70118806-70118825 | Msa0619080:CDS | 50.0% | |
| GTGTGGCTTCTTCACTCCTC+CGG | - | chr4_2:70118625-70118644 | None:intergenic | 55.0% | |
| GCGGCTGAAGCTTACTTGGT+CGG | + | chr4_2:70118810-70118829 | Msa0619080:CDS | 55.0% | |
| ACAGCTCGTAAGTCCACCGG+CGG | + | chr4_2:70118543-70118562 | Msa0619080:CDS | 60.0% | |
| TTTGTGGCGAGCTGCTTCCG+GGG | - | chr4_2:70118576-70118595 | None:intergenic | 60.0% | |
| CTTTGTGGCGAGCTGCTTCC+GGG | - | chr4_2:70118577-70118596 | None:intergenic | 60.0% | |
| CCTTTGTGGCGAGCTGCTTC+CGG | - | chr4_2:70118578-70118597 | None:intergenic | 60.0% | |
| GGCCGCTCGAAAATCAGCTC+CGG | + | chr4_2:70118596-70118615 | Msa0619080:CDS | 60.0% | |
| !! | CGCCGGAGCTGATTTTCGAG+CGG | - | chr4_2:70118601-70118620 | None:intergenic | 60.0% |
| CGAAAATCAGCTCCGGCGAC+CGG | + | chr4_2:70118603-70118622 | Msa0619080:CDS | 60.0% | |
| TTCTTCACTCCTCCGGTCGC+CGG | - | chr4_2:70118618-70118637 | None:intergenic | 60.0% | |
| GAGCCGAAACAGCACTGCTC+TGG | - | chr4_2:70118782-70118801 | None:intergenic | 60.0% | |
| TCGTAAGTCCACCGGCGGCA+AGG | + | chr4_2:70118548-70118567 | Msa0619080:CDS | 65.0% | |
| CCGGAAGCAGCTCGCCACAA+AGG | + | chr4_2:70118575-70118594 | Msa0619080:CDS | 65.0% | |
| AAATCAGCTCCGGCGACCGG+AGG | + | chr4_2:70118606-70118625 | Msa0619080:CDS | 65.0% | |
| CTTCCGGGGAGCCTTGCCGC+CGG | - | chr4_2:70118562-70118581 | None:intergenic | 75.0% | |
| ! | CCACCGGCGGCAAGGCTCCC+CGG | + | chr4_2:70118556-70118575 | Msa0619080:CDS | 80.0% |
| ! | CCGGGGAGCCTTGCCGCCGG+TGG | - | chr4_2:70118559-70118578 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 70118525 | 70118935 | 70118525 | ID=Msa0619080;Name=Msa0619080 |
| chr4_2 | mRNA | 70118525 | 70118935 | 70118525 | ID=Msa0619080-mRNA-1;Parent=Msa0619080;Name=Msa0619080-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr4_2 | exon | 70118525 | 70118935 | 70118525 | ID=Msa0619080-mRNA-1:exon:20380;Parent=Msa0619080-mRNA-1 |
| chr4_2 | CDS | 70118525 | 70118935 | 70118525 | ID=Msa0619080-mRNA-1:cds;Parent=Msa0619080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
