AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603980 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0603980 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0603980 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0603980 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0603980 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0603980 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0603980 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0603980 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0603980 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0603980 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0603980 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0603980 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0603980 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0603980 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0603980 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0603980 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603980 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0603980 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0603980 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0603980 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0603980 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0603980 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0603980 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0603980 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0603980 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0603980 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0603980 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0603980 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0603980 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0603980 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0603980 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0603980 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 38 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGGCTGGGGCGGATTTC+CGG 0.131995 4_2:-49121494 None:intergenic
TTCTTCACTCCGCCGGTGGC+TGG 0.208877 4_2:-49121507 None:intergenic
TTCACGGAGTGCGACGGTTC+CGG 0.309270 4_2:-49121547 None:intergenic
TCACGGAGTGCGACGGTTCC+GGG 0.327175 4_2:-49121546 None:intergenic
TCTTCACTCCGCCGGTGGCT+GGG 0.346533 4_2:-49121506 None:intergenic
GGTGGCTGGGGCGGATTTCC+GGG 0.349417 4_2:-49121493 None:intergenic
TTGAATATCCTTGGGCATAA+TGG 0.390549 4_2:-49121772 None:intergenic
CACGAACGAGACGTTGGAAT+GGG 0.414135 4_2:-49121614 None:intergenic
TTATCTCGTTGGACTCTTTG+AGG 0.419676 4_2:+49121713 Msa0603980:CDS
GAGCAGAAACAGCGCTACTC+TGG 0.427137 4_2:-49121671 None:intergenic
CTTCACTCCGCCGGTGGCTG+GGG 0.429355 4_2:-49121505 None:intergenic
CGGAAATCCGCCCCAGCCAC+CGG 0.439556 4_2:+49121495 Msa0603980:CDS
TTTGGTATTTGCGGATTTCA+CGG 0.451483 4_2:-49121563 None:intergenic
CACTCCGCCGGTGGCTGGGG+CGG 0.455168 4_2:-49121502 None:intergenic
GCGAGCGAGTTGAATATCCT+TGG 0.467952 4_2:-49121781 None:intergenic
CGAGCGAGTTGAATATCCTT+GGG 0.506955 4_2:-49121780 None:intergenic
CGACGGTTCCGGGACGGAAA+CGG 0.513929 4_2:-49121536 None:intergenic
TCACGAACGAGACGTTGGAA+TGG 0.518359 4_2:-49121615 None:intergenic
AAGCCTCACCGTTTCCGTCC+CGG 0.527320 4_2:+49121528 Msa0603980:CDS
TCGGGGAGCTTTGCCTCCGG+TGG 0.527426 4_2:-49121448 None:intergenic
AAACAGCGCTACTCTGGAAA+CGG 0.532210 4_2:-49121665 None:intergenic
GCTGCTGAAGCTTATCTCGT+TGG 0.533530 4_2:+49121702 Msa0603980:CDS
GCTACTCTGGAAACGGAGAT+CGG 0.538527 4_2:-49121658 None:intergenic
CAATTTCACGAACGAGACGT+TGG 0.552469 4_2:-49121620 None:intergenic
GGCTGGGGCGGATTTCCGGG+CGG 0.553153 4_2:-49121490 None:intergenic
CAACTCGCTCGCAGAATCAG+AGG 0.556058 4_2:+49121792 Msa0603980:CDS
GTGAGGCTTCTTCACTCCGC+CGG 0.560763 4_2:-49121514 None:intergenic
AGGCTTCTTCACTCCGCCGG+TGG 0.575034 4_2:-49121511 None:intergenic
TCGCAGAATCAGAGGCGAGA+GGG 0.584947 4_2:+49121800 Msa0603980:CDS
CAAACAGCTCGCAAATCCAC+CGG 0.586123 4_2:+49121432 Msa0603980:CDS
AGCTAGCGACAAAAGCCGCC+CGG 0.586742 4_2:+49121475 Msa0603980:CDS
GCGGATTTCACGGAGTGCGA+CGG 0.595387 4_2:-49121553 None:intergenic
CTCGCAGAATCAGAGGCGAG+AGG 0.599896 4_2:+49121799 Msa0603980:CDS
GAGAGTTACCATTATGCCCA+AGG 0.602637 4_2:+49121764 Msa0603980:CDS
AAATCCGCCCCAGCCACCGG+CGG 0.628636 4_2:+49121498 Msa0603980:CDS
GGAGTGCGACGGTTCCGGGA+CGG 0.649162 4_2:-49121542 None:intergenic
GTTCCGGGACGGAAACGGTG+AGG 0.691461 4_2:-49121531 None:intergenic
ACAGCTCGCAAATCCACCGG+AGG 0.704783 4_2:+49121435 Msa0603980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTTGGTATTTGCGGATTTCA+CGG - chr4_2:49121566-49121585 None:intergenic 35.0%
!!! CTGTGCTTTTTTGGTATTTG+CGG - chr4_2:49121575-49121594 None:intergenic 35.0%
TTGAATATCCTTGGGCATAA+TGG - chr4_2:49121775-49121794 None:intergenic 35.0%
!! TTTTGTCGCTAGCTGTTTTC+GGG - chr4_2:49121469-49121488 None:intergenic 40.0%
!! CTTTTGTCGCTAGCTGTTTT+CGG - chr4_2:49121470-49121489 None:intergenic 40.0%
!!! TGAGAAGCTCTGTGCTTTTT+TGG - chr4_2:49121584-49121603 None:intergenic 40.0%
TTATCTCGTTGGACTCTTTG+AGG + chr4_2:49121713-49121732 Msa0603980:CDS 40.0%
!! TTTGTCGCTAGCTGTTTTCG+GGG - chr4_2:49121468-49121487 None:intergenic 45.0%
! GACGTTGGAATGGGAGTTTT+CGG - chr4_2:49121608-49121627 None:intergenic 45.0%
CAATTTCACGAACGAGACGT+TGG - chr4_2:49121623-49121642 None:intergenic 45.0%
! AAACAGCGCTACTCTGGAAA+CGG - chr4_2:49121668-49121687 None:intergenic 45.0%
GAGAGTTACCATTATGCCCA+AGG + chr4_2:49121764-49121783 Msa0603980:CDS 45.0%
! CGAGCGAGTTGAATATCCTT+GGG - chr4_2:49121783-49121802 None:intergenic 45.0%
CAAACAGCTCGCAAATCCAC+CGG + chr4_2:49121432-49121451 Msa0603980:CDS 50.0%
CACGAACGAGACGTTGGAAT+GGG - chr4_2:49121617-49121636 None:intergenic 50.0%
TCACGAACGAGACGTTGGAA+TGG - chr4_2:49121618-49121637 None:intergenic 50.0%
! GCTACTCTGGAAACGGAGAT+CGG - chr4_2:49121661-49121680 None:intergenic 50.0%
GCTGCTGAAGCTTATCTCGT+TGG + chr4_2:49121702-49121721 Msa0603980:CDS 50.0%
! GCGAGCGAGTTGAATATCCT+TGG - chr4_2:49121784-49121803 None:intergenic 50.0%
!! TTTTCGGGGAGCTTTGCCTC+CGG - chr4_2:49121454-49121473 None:intergenic 55.0%
GAGCAGAAACAGCGCTACTC+TGG - chr4_2:49121674-49121693 None:intergenic 55.0%
CAACTCGCTCGCAGAATCAG+AGG + chr4_2:49121792-49121811 Msa0603980:CDS 55.0%
TCGCAGAATCAGAGGCGAGA+GGG + chr4_2:49121800-49121819 Msa0603980:CDS 55.0%
ACAGCTCGCAAATCCACCGG+AGG + chr4_2:49121435-49121454 Msa0603980:CDS 60.0%
AGCTAGCGACAAAAGCCGCC+CGG + chr4_2:49121475-49121494 Msa0603980:CDS 60.0%
GTGAGGCTTCTTCACTCCGC+CGG - chr4_2:49121517-49121536 None:intergenic 60.0%
AAGCCTCACCGTTTCCGTCC+CGG + chr4_2:49121528-49121547 Msa0603980:CDS 60.0%
TTCACGGAGTGCGACGGTTC+CGG - chr4_2:49121550-49121569 None:intergenic 60.0%
GCGGATTTCACGGAGTGCGA+CGG - chr4_2:49121556-49121575 None:intergenic 60.0%
CTCGCAGAATCAGAGGCGAG+AGG + chr4_2:49121799-49121818 Msa0603980:CDS 60.0%
TCTTCACTCCGCCGGTGGCT+GGG - chr4_2:49121509-49121528 None:intergenic 65.0%
TTCTTCACTCCGCCGGTGGC+TGG - chr4_2:49121510-49121529 None:intergenic 65.0%
AGGCTTCTTCACTCCGCCGG+TGG - chr4_2:49121514-49121533 None:intergenic 65.0%
GTTCCGGGACGGAAACGGTG+AGG - chr4_2:49121534-49121553 None:intergenic 65.0%
CGACGGTTCCGGGACGGAAA+CGG - chr4_2:49121539-49121558 None:intergenic 65.0%
TCACGGAGTGCGACGGTTCC+GGG - chr4_2:49121549-49121568 None:intergenic 65.0%
TCGGGGAGCTTTGCCTCCGG+TGG - chr4_2:49121451-49121470 None:intergenic 70.0%
! GGTGGCTGGGGCGGATTTCC+GGG - chr4_2:49121496-49121515 None:intergenic 70.0%
! CGGTGGCTGGGGCGGATTTC+CGG - chr4_2:49121497-49121516 None:intergenic 70.0%
CGGAAATCCGCCCCAGCCAC+CGG + chr4_2:49121495-49121514 Msa0603980:CDS 70.0%
AAATCCGCCCCAGCCACCGG+CGG + chr4_2:49121498-49121517 Msa0603980:CDS 70.0%
CTTCACTCCGCCGGTGGCTG+GGG - chr4_2:49121508-49121527 None:intergenic 70.0%
GGAGTGCGACGGTTCCGGGA+CGG - chr4_2:49121545-49121564 None:intergenic 70.0%
! GGCTGGGGCGGATTTCCGGG+CGG - chr4_2:49121493-49121512 None:intergenic 75.0%
! CACTCCGCCGGTGGCTGGGG+CGG - chr4_2:49121505-49121524 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 49121417 49121827 49121417 ID=Msa0603980;Name=Msa0603980
chr4_2 mRNA 49121417 49121827 49121417 ID=Msa0603980-mRNA-1;Parent=Msa0603980;Name=Msa0603980-mRNA-1;_AED=0.41;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|136
chr4_2 exon 49121417 49121827 49121417 ID=Msa0603980-mRNA-1:exon:11353;Parent=Msa0603980-mRNA-1
chr4_2 CDS 49121417 49121827 49121417 ID=Msa0603980-mRNA-1:cds;Parent=Msa0603980-mRNA-1
Gene Sequence

>Msa0603980

ATGGCGCGTACAAAGCAAACAGCTCGCAAATCCACCGGAGGCAAAGCTCCCCGAAAACAGCTAGCGACAAAAGCCGCCCGGAAATCCGCCCCAGCCACCGGCGGAGTGAAGAAGCCTCACCGTTTCCGTCCCGGAACCGTCGCACTCCGTGAAATCCGCAAATACCAAAAAAGCACAGAGCTTCTCATCCGAAAACTCCCATTCCAACGTCTCGTTCGTGAAATTGCTCAAGATTTCAAAACCGATCTCCGTTTCCAGAGTAGCGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTCGTTGGACTCTTTGAGGATACGAATCTTTGTGCTATTCATGCTAAGAGAGTTACCATTATGCCCAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGGGCTTAA

Protein sequence

>Msa0603980

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*