Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001830 | Msa0764920 | 0.818090 | 2.349550e-52 | -8.615850e-47 |
| Msa0003630 | Msa0764920 | 0.839389 | 1.631794e-57 | -8.615850e-47 |
| Msa0010490 | Msa0764920 | 0.826913 | 2.089453e-54 | -8.615850e-47 |
| Msa0010500 | Msa0764920 | 0.803345 | 3.643261e-49 | -8.615850e-47 |
| Msa0010800 | Msa0764920 | 0.801763 | 7.723971e-49 | -8.615850e-47 |
| Msa0012710 | Msa0764920 | 0.831699 | 1.439779e-55 | -8.615850e-47 |
| Msa0015090 | Msa0764920 | 0.829138 | 6.086182e-55 | -8.615850e-47 |
| Msa0017320 | Msa0764920 | 0.846141 | 2.617504e-59 | -8.615850e-47 |
| Msa0020390 | Msa0764920 | 0.802322 | 5.926869e-49 | -8.615850e-47 |
| Msa0033850 | Msa0764920 | 0.813308 | 2.733330e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0764920 | MtrunA17_Chr5g0415541 | 95.868 | 484 | 19 | 1 | 1 | 483 | 1 | 484 | 0.0 | 964 |
| Msa0764920 | MtrunA17_Chr8g0363721 | 57.617 | 512 | 175 | 9 | 1 | 483 | 1 | 499 | 0.0 | 570 |
| Msa0764920 | MtrunA17_Chr6g0452681 | 52.090 | 311 | 110 | 6 | 203 | 477 | 1 | 308 | 1.01e-86 | 270 |
| Msa0764920 | MtrunA17_Chr6g0452681 | 47.682 | 151 | 74 | 3 | 1 | 149 | 1 | 148 | 2.28e-36 | 137 |
| Msa0764920 | MtrunA17_Chr2g0280871 | 56.771 | 192 | 77 | 2 | 203 | 394 | 1 | 186 | 3.08e-68 | 219 |
| Msa0764920 | MtrunA17_Chr2g0280871 | 47.020 | 151 | 73 | 3 | 1 | 149 | 1 | 146 | 1.16e-36 | 135 |
| Msa0764920 | MtrunA17_Chr8g0363091 | 67.105 | 76 | 25 | 0 | 384 | 459 | 14 | 89 | 5.17e-28 | 108 |
| Msa0764920 | MtrunA17_Chr8g0362891 | 65.333 | 75 | 26 | 0 | 384 | 458 | 14 | 88 | 4.02e-27 | 104 |
| Msa0764920 | MtrunA17_Chr2g0280861 | 53.191 | 94 | 42 | 2 | 384 | 477 | 4 | 95 | 1.48e-25 | 101 |
| Msa0764920 | MtrunA17_Chr8g0363181 | 55.556 | 90 | 29 | 2 | 304 | 387 | 11 | 95 | 4.16e-23 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0764920 | AT1G27100.1 | 49.237 | 524 | 221 | 11 | 1 | 484 | 1 | 519 | 1.88e-155 | 454 |
| Msa0764920 | AT1G59710.2 | 61.565 | 294 | 100 | 4 | 203 | 483 | 1 | 294 | 2.97e-116 | 347 |
| Msa0764920 | AT1G59710.2 | 47.682 | 151 | 73 | 3 | 1 | 149 | 1 | 147 | 1.24e-34 | 133 |
| Msa0764920 | AT1G59710.1 | 61.775 | 293 | 99 | 4 | 203 | 482 | 1 | 293 | 5.96e-116 | 345 |
| Msa0764920 | AT1G59710.1 | 47.682 | 151 | 73 | 3 | 1 | 149 | 1 | 147 | 1.46e-34 | 132 |
| Msa0764920 | AT3G28630.1 | 56.291 | 302 | 112 | 5 | 200 | 483 | 30 | 329 | 9.60e-104 | 315 |
| Msa0764920 | AT3G28630.1 | 45.638 | 149 | 74 | 3 | 1 | 147 | 33 | 176 | 1.44e-33 | 130 |
| Msa0764920 | AT3G28630.2 | 57.095 | 296 | 105 | 4 | 200 | 483 | 30 | 315 | 3.02e-103 | 313 |
| Msa0764920 | AT3G28630.2 | 45.638 | 149 | 74 | 3 | 1 | 147 | 33 | 176 | 1.24e-33 | 130 |
| Msa0764920 | AT1G69890.1 | 55.914 | 279 | 115 | 3 | 203 | 477 | 1 | 275 | 4.59e-101 | 306 |
Find 141 sgRNAs with CRISPR-Local
Find 449 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCCAACAAGCTTCGAAG+CGG | 0.642582 | 5_2:+25392739 | Msa0764920:CDS |
| AAACTCATAACCAAGTAATA+AGG | 0.369988 | 5_2:+25387397 | Msa0764920:CDS |
| AAAGTCCGTCAAAGCCGAAA+CGG | 0.559086 | 5_2:+25387327 | Msa0764920:CDS |
| AAATGCAATGGACTATTACA+AGG | 0.595945 | 5_2:-25394976 | None:intergenic |
| AAGAAGATTGAGGAAGAGAC+AGG | 0.469690 | 5_2:+25394105 | Msa0764920:CDS |
| AAGCTAGCAGAAACACTAGG+AGG | 0.716084 | 5_2:-25393282 | None:intergenic |
| AATCTGCTAAACAAATGCAA+TGG | 0.595832 | 5_2:-25394988 | None:intergenic |
| AATGGCAGCCAATACGTGAC+GGG | 0.583517 | 5_2:+25387535 | Msa0764920:CDS |
| ACACGAACACCATCCCTTAC+AGG | 0.328669 | 5_2:-25393048 | None:intergenic |
| ACAGAAGAATCCAAAGTCTT+TGG | 0.280690 | 5_2:-25393015 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTATAGTATCAAACCATTCA+TGG | + | chr5_2:25387227-25387246 | Msa0764920:exon | 30.0% | |
| TTGGTGAAATATTCCATGAA+TGG | - | chr5_2:25387243-25387262 | None:intergenic | 30.0% | |
| GAGCTTAATGATCTTTGATT+TGG | - | chr5_2:25387262-25387281 | None:intergenic | 30.0% | |
| GACTCTTTAACCTTATTACT+TGG | - | chr5_2:25387410-25387429 | None:intergenic | 30.0% | |
| ATAAGGTTAAAGAGTCACAA+CGG | + | chr5_2:25387414-25387433 | Msa0764920:CDS | 30.0% | |
| AGGAAAGAGGGTAATATTAA+CGG | + | chr5_2:25387482-25387501 | Msa0764920:CDS | 30.0% | |
| TGATGATTCGTTTGTGAAAA+TGG | + | chr5_2:25387641-25387660 | Msa0764920:CDS | 30.0% | |
| TTTCGTAACCTCAATATACT+AGG | + | chr5_2:25388511-25388530 | Msa0764920:intron | 30.0% | |
| TCTTCGATGAAATATCTGTT+TGG | + | chr5_2:25389102-25389121 | Msa0764920:intron | 30.0% | |
| CTTCGATGAAATATCTGTTT+GGG | + | chr5_2:25389103-25389122 | Msa0764920:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 25387070 | 25395247 | 25387070 | ID=Msa0764920;Name=Msa0764920 |
| chr5_2 | mRNA | 25387070 | 25395247 | 25387070 | ID=Msa0764920-mRNA-1;Parent=Msa0764920;Name=Msa0764920-mRNA-1;_AED=0.05;_eAED=0.05;_QI=176|0.5|0.8|0.8|0.5|0.8|5|285|543 |
| chr5_2 | exon | 25387070 | 25387821 | 25387070 | ID=Msa0764920-mRNA-1:exon:10232;Parent=Msa0764920-mRNA-1 |
| chr5_2 | exon | 25392733 | 25393317 | 25392733 | ID=Msa0764920-mRNA-1:exon:10233;Parent=Msa0764920-mRNA-1 |
| chr5_2 | exon | 25393964 | 25394249 | 25393964 | ID=Msa0764920-mRNA-1:exon:10234;Parent=Msa0764920-mRNA-1 |
| chr5_2 | exon | 25394680 | 25394726 | 25394680 | ID=Msa0764920-mRNA-1:exon:10235;Parent=Msa0764920-mRNA-1 |
| chr5_2 | exon | 25394825 | 25395247 | 25394825 | ID=Msa0764920-mRNA-1:exon:10236;Parent=Msa0764920-mRNA-1 |
| chr5_2 | five_prime_UTR | 25387070 | 25387245 | 25387070 | ID=Msa0764920-mRNA-1:five_prime_utr;Parent=Msa0764920-mRNA-1 |
| chr5_2 | CDS | 25387246 | 25387821 | 25387246 | ID=Msa0764920-mRNA-1:cds;Parent=Msa0764920-mRNA-1 |
| chr5_2 | CDS | 25392733 | 25393317 | 25392733 | ID=Msa0764920-mRNA-1:cds;Parent=Msa0764920-mRNA-1 |
| chr5_2 | CDS | 25393964 | 25394249 | 25393964 | ID=Msa0764920-mRNA-1:cds;Parent=Msa0764920-mRNA-1 |
| chr5_2 | CDS | 25394680 | 25394726 | 25394680 | ID=Msa0764920-mRNA-1:cds;Parent=Msa0764920-mRNA-1 |
| chr5_2 | CDS | 25394825 | 25394962 | 25394825 | ID=Msa0764920-mRNA-1:cds;Parent=Msa0764920-mRNA-1 |
| chr5_2 | three_prime_UTR | 25394963 | 25395247 | 25394963 | ID=Msa0764920-mRNA-1:three_prime_utr;Parent=Msa0764920-mRNA-1 |
| Gene Sequence |
>Msa0764920 ATGGAATATTTCACCAAATCAAAGATCATTAAGCTCAAAAGCCACCTAGACAAATACCTTATCGCCGACGACAACAACGGAAAAGTCCGTCAAAGCCGAAACGGAGCCATTAAAAGAGCTCAATGGACAGTGGAGATAGTGCAAAAACTACAAACTCATAACCAAGTAATAAGGTTAAAGAGTCACAACGGAAAATATTTAACGGCGACAGAAACACCGTATCCCTTAACCCTAACAGGAAAGAGGGTAATATTAACGGAGTTAGAAAACGGTGTAGATGGTAAAAACGAATGGCAGCCAATACGTGACGGGTTTCAGGTGAAGCTGAGGAGTTGTTGTGGGAGGTTTTTGAAGGGAAACGGTAGTAATCCGCCGTGGAGGAACAGTGTAACGATTGATGATTCGTTTGTGAAAATGGAGGGTGTTTTGTGGGATGTTGAAGGTGTTATGGAAGATGAGTTTGAGAGTTTGTTATCTTCTTTTGGTTCTGATGATGTTTCTTTTGCGTCGGATATTCCGTCGCCGATGAGTGTTTTCTCACTTGGGTCGTCGCCGCCGGCGAGAGGAAGTTTTCAGACAACTAAACCCAACAAGCTTCGAAGCGGAATGGACTTCTTCCACAAAGCAAAAGCAGTCCGTCTCAGAAGCCACCACGACAAATATCTCTTAGCAGAAGAAGATGAAGAATCCGTTTCACAAGGAAGAAACGGTTCATCAAACAACGCAAGATGGTACGTAGAATATGTCCCAAACTACGACAACGTTATCCGTCTCAAAAGCTGTTACGGCAAATACCTAACAGCATCAGACACTCCATTTCTTCTCGGCATGACTGGTCGAAAAGTGCTTCAAACTAGTCCAAAGACTTTGGATTCTTCTGTTGAATGGGAGCCTGTAAGGGATGGTGTTCGTGTGAAGCTGAAGACGAGGTATGGGAATTTTCTTAGAGGGAATGGTGGGTTGCCACCGTGGAGGAATACTGTGACGCATGATATTCCGCATAGGACTGTTACTCAGGATTGGGTTTTGTGGGATGTTGATGTTGTTGAGATTCATGTTAGTAAACAACAACCTTCTGCTCCAGCTTTTGAAACTAGCATGCCTTCTCCTGCTGTGGCGGTTGCTCCTCCTAGTGTTTCTGCTAGCTTTTCTAGACAACAGTCGAATGATGATGCATCACCTAGGAAGATGGAGGGGCGAATTATATACTACCATGTTGCTGAAGATGATGGAGAAGTGAGTGATGAAGGTGTGCAGGGATATTCTTTGGTTTTCAAAGGGAATGGAGTTGAGGAATTGAGCAAGAAGATTGAGGAAGAGACAGGGTTAGAGGGAATCATTGTGTGTACTAAGAGTCCTTTGAATGGAAAGCTTTATCCTCTTCGTTTGCAGCTTCCTCCAAATAATGTTACTATGCAGGTTGTTTTGGTTCTTCCTTTCTCAAAAGCATTGTGCCCTGAAATATTTGGTTGTTCTGATTTGAGTGCTTGCATTTGGCAAGAGAGTTGGAGGCACAAGGTTATCTTTGAGAAGCCAGATTTTGCAACACATGGCCTTTGTACATACTACATACTGATTCAAGCTTCTGAGAGTTATATGTTAAAATATTTAGGATTCGAGAATGTGTTATAG |
| Protein sequence |
>Msa0764920 MEYFTKSKIIKLKSHLDKYLIADDNNGKVRQSRNGAIKRAQWTVEIVQKLQTHNQVIRLKSHNGKYLTATETPYPLTLTGKRVILTELENGVDGKNEWQPIRDGFQVKLRSCCGRFLKGNGSNPPWRNSVTIDDSFVKMEGVLWDVEGVMEDEFESLLSSFGSDDVSFASDIPSPMSVFSLGSSPPARGSFQTTKPNKLRSGMDFFHKAKAVRLRSHHDKYLLAEEDEESVSQGRNGSSNNARWYVEYVPNYDNVIRLKSCYGKYLTASDTPFLLGMTGRKVLQTSPKTLDSSVEWEPVRDGVRVKLKTRYGNFLRGNGGLPPWRNTVTHDIPHRTVTQDWVLWDVDVVEIHVSKQQPSAPAFETSMPSPAVAVAPPSVSASFSRQQSNDDASPRKMEGRIIYYHVAEDDGEVSDEGVQGYSLVFKGNGVEELSKKIEEETGLEGIIVCTKSPLNGKLYPLRLQLPPNNVTMQVVLVLPFSKALCPEIFGCSDLSACIWQESWRHKVIFEKPDFATHGLCTYYILIQASESYMLKYLGFENVL* |
