AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0059960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0059960 MtrunA17_Chr1g0159461 97.952 586 9 1 1 583 1 586 0.0 1189
Msa0059960 MtrunA17_Chr3g0125811 69.646 593 136 8 1 583 6 564 0.0 790
Msa0059960 MtrunA17_Chr5g0447691 56.583 357 155 0 223 579 175 531 2.48e-153 451
Msa0059960 MtrunA17_Chr2g0319091 42.830 523 286 3 70 579 44 566 2.57e-142 424
Msa0059960 MtrunA17_Chr3g0101141 50.700 357 176 0 223 579 173 529 2.66e-136 407
Msa0059960 MtrunA17_Chr5g0445331 44.589 462 222 7 123 579 87 519 3.78e-131 394
Msa0059960 MtrunA17_Chr4g0076091 42.825 446 223 6 137 579 114 530 6.95e-127 383
Msa0059960 MtrunA17_Chr2g0290241 48.276 348 179 1 223 569 55 402 4.66e-122 367
Msa0059960 MtrunA17_Chr7g0236851 37.684 475 254 12 123 579 194 644 1.15e-75 253
Msa0059960 MtrunA17_Chr7g0224271 38.667 375 198 6 224 579 299 660 7.39e-73 246
Msa0059960 MtrunA17_Chr6g0468561 38.624 378 190 10 223 579 293 649 2.63e-68 233
Msa0059960 MtrunA17_Chr3g0110971 35.676 370 207 8 226 579 220 574 5.12e-62 215
Msa0059960 MtrunA17_Chr4g0031721 33.333 351 233 1 223 572 314 664 1.73e-60 213
Msa0059960 MtrunA17_Chr7g0245601 37.677 353 205 6 228 579 441 779 8.78e-60 213
Msa0059960 MtrunA17_Chr7g0238891 30.227 397 264 2 189 572 323 719 7.06e-59 209
Msa0059960 MtrunA17_Chr8g0376381 35.989 364 199 11 226 579 170 509 2.34e-58 204
Msa0059960 MtrunA17_Chr2g0306641 34.793 411 235 13 177 581 93 476 2.59e-58 203
Msa0059960 MtrunA17_Chr4g0031741 33.333 351 230 3 225 574 284 631 6.13e-57 202
Msa0059960 MtrunA17_Chr8g0357601 33.973 365 222 7 226 579 183 539 4.58e-56 198
Msa0059960 MtrunA17_Chr4g0031731 31.016 374 254 2 203 572 232 605 1.17e-55 198
Msa0059960 MtrunA17_Chr4g0031701 29.947 374 258 2 203 572 232 605 3.13e-55 197
Msa0059960 MtrunA17_Chr4g0057891 31.978 369 229 5 224 579 148 507 5.02e-55 194
Msa0059960 MtrunA17_Chr2g0328381 31.176 340 229 2 223 561 273 608 5.40e-54 194
Msa0059960 MtrunA17_Chr4g0031711 29.945 364 251 2 203 562 339 702 1.47e-53 194
Msa0059960 MtrunA17_Chr2g0328341 31.085 341 230 2 223 562 270 606 7.99e-53 191
Msa0059960 MtrunA17_Chr2g0328391 30.226 354 241 3 223 572 297 648 1.84e-52 190
Msa0059960 MtrunA17_Chr3g0085681 28.715 397 227 4 226 572 26 416 1.86e-52 185
Msa0059960 MtrunA17_Chr4g0038761 35.475 358 210 10 228 579 87 429 4.05e-50 179
Msa0059960 MtrunA17_Chr4g0057291 34.551 356 210 12 225 569 292 635 6.82e-50 183
Msa0059960 MtrunA17_Chr2g0328411 28.496 379 259 3 187 562 254 623 7.01e-50 183
Msa0059960 MtrunA17_Chr5g0396491 28.883 412 226 7 224 574 55 460 3.37e-49 177
Msa0059960 MtrunA17_Chr5g0401131 31.044 364 233 8 220 570 109 467 8.88e-49 177
Msa0059960 MtrunA17_Chr2g0328431 28.462 390 270 6 187 574 253 635 1.34e-48 179
Msa0059960 MtrunA17_Chr2g0328401 28.000 350 249 1 225 574 290 636 4.04e-47 175
Msa0059960 MtrunA17_Chr4g0039661 31.429 350 226 6 226 571 194 533 4.07e-45 168
Msa0059960 MtrunA17_Chr3g0114841 32.527 372 218 10 224 574 119 478 2.16e-44 165
Msa0059960 MtrunA17_Chr2g0329591 30.623 369 231 7 224 577 73 431 4.33e-42 157
Msa0059960 MtrunA17_Chr1g0200401 30.707 368 223 11 224 575 79 430 1.85e-41 155
Msa0059960 MtrunA17_Chr2g0328361 29.619 341 208 6 223 561 272 582 3.02e-41 158
Msa0059960 MtrunA17_Chr1g0183641 31.694 366 215 12 225 579 123 464 4.32e-41 155
Msa0059960 MtrunA17_Chr3g0085361 27.744 328 182 3 293 571 9 330 3.62e-39 147
Msa0059960 MtrunA17_Chr2g0328371 32.271 251 165 2 296 545 2 248 3.70e-39 144
Msa0059960 MtrunA17_Chr5g0401421 27.248 367 219 7 223 543 33 397 1.60e-36 141
Msa0059960 MtrunA17_Chr5g0424131 30.233 430 277 15 161 574 57 479 3.12e-36 142
Msa0059960 MtrunA17_Chr4g0053901 28.297 364 217 7 223 543 57 419 7.62e-33 131
Msa0059960 MtrunA17_Chr4g0038851 32.444 225 145 2 224 447 47 265 2.45e-30 124
Msa0059960 MtrunA17_Chr4g0038851 40.152 132 78 1 444 574 320 451 7.46e-25 108
Msa0059960 MtrunA17_Chr2g0294461 24.100 361 258 5 226 575 211 566 7.50e-29 121
Msa0059960 MtrunA17_Chr2g0294471 25.893 336 228 7 226 549 205 531 2.32e-26 113
Msa0059960 MtrunA17_Chr7g0230731 27.907 344 221 11 226 562 373 696 1.51e-22 102
Msa0059960 MtrunA17_Chr8g0383981 28.736 348 205 14 225 555 139 460 3.15e-22 100
Msa0059960 MtrunA17_Chr7g0230771 28.198 344 220 11 226 562 376 699 5.91e-22 100
Msa0059960 MtrunA17_Chr4g0012071 26.796 362 243 11 205 562 176 519 1.20e-21 99.0
Msa0059960 MtrunA17_Chr8g0344101 24.868 378 243 15 220 572 218 579 2.14e-21 98.2
Msa0059960 MtrunA17_Chr7g0270031 24.540 326 229 8 224 541 188 504 8.61e-20 93.2
Msa0059960 MtrunA17_Chr2g0323111 27.320 194 121 3 398 572 94 286 5.99e-18 85.1
Msa0059960 MtrunA17_Chr1g0194621 26.556 241 160 7 315 548 247 477 6.92e-16 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0059960 AT1G21450.1 61.630 589 190 14 1 578 16 579 0.0 671
Msa0059960 AT5G48150.1 51.810 442 191 6 147 579 49 477 2.43e-153 449
Msa0059960 AT5G48150.4 51.810 442 191 6 147 579 49 477 2.43e-153 449
Msa0059960 AT5G48150.2 51.810 442 191 6 147 579 49 477 2.43e-153 449
Msa0059960 AT5G48150.3 51.810 442 191 6 147 579 49 477 2.43e-153 449
Msa0059960 AT1G50600.3 54.342 357 163 0 223 579 157 513 9.84e-140 416
Msa0059960 AT1G50600.1 54.342 357 163 0 223 579 228 584 8.94e-139 416
Msa0059960 AT1G50600.2 54.342 357 163 0 223 579 232 588 1.14e-138 416
Msa0059960 AT2G04890.1 53.782 357 158 1 223 579 51 400 3.46e-136 403
Msa0059960 AT4G17230.1 51.404 356 173 0 223 578 156 511 2.05e-128 387
Msa0059960 AT4G17230.2 51.404 356 173 0 223 578 156 511 2.05e-128 387
Msa0059960 AT5G52510.1 38.441 372 204 8 223 579 266 627 1.54e-72 245
Msa0059960 AT3G03450.1 37.466 363 204 9 226 579 184 532 7.19e-65 222
Msa0059960 AT1G07530.1 35.411 353 227 1 223 574 394 746 1.06e-64 226
Msa0059960 AT1G66350.1 36.719 384 216 10 202 579 131 493 6.86e-63 216
Msa0059960 AT3G54220.1 37.535 357 203 8 228 579 296 637 7.07e-61 214
Msa0059960 AT5G59450.1 31.694 366 235 4 203 562 211 567 4.34e-59 208
Msa0059960 AT5G17490.1 38.889 324 177 8 224 543 159 465 6.25e-59 205
Msa0059960 AT2G29060.2 30.238 420 276 4 148 560 212 621 1.28e-57 205
Msa0059960 AT2G29060.1 30.238 420 276 4 148 560 249 658 2.22e-57 205
Msa0059960 AT1G14920.1 34.722 360 213 9 226 579 173 516 6.93e-56 197
Msa0059960 AT5G66770.1 35.393 356 216 7 226 576 221 567 4.02e-55 196
Msa0059960 AT2G01570.1 34.426 366 206 11 226 579 225 568 1.51e-52 189
Msa0059960 AT2G37650.2 29.714 350 244 1 223 572 333 680 5.53e-52 190
Msa0059960 AT2G37650.1 29.714 350 244 1 223 572 345 692 6.53e-52 190
Msa0059960 AT3G46600.2 30.372 349 242 1 223 571 2 349 7.22e-52 182
Msa0059960 AT3G46600.1 29.863 365 255 1 207 571 194 557 3.35e-51 186
Msa0059960 AT3G46600.3 29.863 365 255 1 207 571 162 525 5.42e-51 184
Msa0059960 AT2G29065.1 31.322 348 232 5 223 563 250 597 1.08e-50 185
Msa0059960 AT1G07520.2 30.460 348 234 6 223 563 152 498 3.32e-49 179
Msa0059960 AT1G07520.4 30.460 348 234 6 223 563 152 498 3.32e-49 179
Msa0059960 AT1G07520.1 30.460 348 234 6 223 563 316 662 8.96e-49 181
Msa0059960 AT1G07520.3 30.460 348 234 6 223 563 317 663 9.89e-49 181
Msa0059960 AT4G37650.1 29.723 397 238 10 191 563 118 497 2.55e-48 177
Msa0059960 AT5G41920.1 31.068 412 247 11 175 579 6 387 1.11e-47 172
Msa0059960 AT3G50650.1 29.322 457 271 10 157 576 84 525 1.48e-45 169
Msa0059960 AT1G63100.1 32.565 347 214 7 225 563 252 586 1.71e-43 165
Msa0059960 AT1G63100.2 32.565 347 214 7 225 563 277 611 2.19e-43 165
Msa0059960 AT1G55580.1 28.010 382 225 8 223 563 42 414 2.68e-37 144
Msa0059960 AT4G08250.1 28.533 368 242 9 224 579 106 464 3.47e-35 139
Msa0059960 AT3G49950.1 28.824 340 213 10 223 543 28 357 3.37e-34 134
Msa0059960 AT1G50420.1 35.714 210 126 2 224 430 56 259 1.19e-33 134
Msa0059960 AT1G50420.1 39.098 133 78 2 444 574 330 461 1.13e-22 102
Msa0059960 AT3G13840.1 26.503 366 240 10 220 574 143 490 1.94e-23 104
Msa0059960 AT5G67411.1 33.668 199 119 7 342 529 2 198 5.81e-18 83.2
Msa0059960 AT3G60630.1 24.699 332 217 10 258 580 291 598 2.84e-17 85.9
Msa0059960 AT2G45160.1 26.619 278 178 8 295 562 346 607 4.18e-16 82.4
Msa0059960 AT4G00150.1 24.484 339 222 12 230 562 212 522 4.79e-14 75.5

Find 164 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAAATCTGATTCAAAAT+TGG 0.173475 1_2:-17505039 None:intergenic
CAGGCAGTTGGTTTCAATTC+AGG 0.191357 1_2:+17505427 Msa0059960:CDS
TCCACAACTGTCACAAGTTT+TGG 0.256449 1_2:-17506046 None:intergenic
TAGAAGCAAGTGTTTGTATC+AGG 0.258688 1_2:-17505718 None:intergenic
CTGATAGAACCGTCTAGTTC+TGG 0.260300 1_2:+17504882 Msa0059960:CDS
TGCCAAGCTATACTCGTTAA+AGG 0.281634 1_2:+17504731 Msa0059960:CDS
TGATACAAACACTTGCTTCT+AGG 0.282847 1_2:+17505720 Msa0059960:CDS
AACAGATCGTTGCACGGATT+CGG 0.283044 1_2:-17505786 None:intergenic
CATTTCTAGCTACATCATTC+AGG 0.286512 1_2:+17503466 Msa0059960:five_prime_UTR
GGAAGGACTTGCGGCTCGTT+TGG 0.307317 1_2:+17505484 Msa0059960:CDS
ACAGATCGTTGCACGGATTC+GGG 0.312714 1_2:-17505785 None:intergenic
GCGATTGAAAATACAAGAAT+TGG 0.316595 1_2:+17505103 Msa0059960:CDS
GCGGCTCGTTTGGCTTCATC+GGG 0.324337 1_2:+17505494 Msa0059960:CDS
AGCTAATTCAGGAGGTTCTA+TGG 0.328878 1_2:+17504956 Msa0059960:CDS
TGATATTTCTTTGGTGCTAC+TGG 0.337052 1_2:-17505306 None:intergenic
GGTATGAAGTTGCCGGAAAA+TGG 0.337471 1_2:+17506263 Msa0059960:CDS
AGCAAATATTTGTTGGTAGA+AGG 0.344190 1_2:-17503433 None:intergenic
TCTTTGACATTTGTGCTCAT+TGG 0.345552 1_2:-17506322 None:intergenic
CACAGCAGAGTAGTAATTAT+AGG 0.347958 1_2:-17506119 None:intergenic
GTACAAGATAATAGAGGAAA+TGG 0.348833 1_2:+17506381 Msa0059960:CDS
TGCGGCTCGTTTGGCTTCAT+CGG 0.352118 1_2:+17505493 Msa0059960:CDS
TTATAGGCTTCAACAAATCT+TGG 0.356310 1_2:-17506103 None:intergenic
CCGTCTAGTTCTGGGATATC+AGG 0.356405 1_2:+17504891 Msa0059960:CDS
GTAGAAGGAAGCTGAGAAAA+TGG 0.381625 1_2:-17503418 None:intergenic
TTTGGATTTATTGCTGCTAA+TGG 0.383067 1_2:+17505614 Msa0059960:CDS
TACATGCTTCTATGCTTATC+AGG 0.384672 1_2:-17504795 None:intergenic
CCTGATTGCTTTGAATTTGA+CGG 0.401860 1_2:+17505074 Msa0059960:CDS
CCGTCAAATTCAAAGCAATC+AGG 0.405445 1_2:-17505074 None:intergenic
ATGGAGGGCAAGGATGAAAA+TGG 0.406129 1_2:+17506282 Msa0059960:CDS
CACTCTTCCGAGAGAGAGCC+AGG 0.410423 1_2:+17506159 Msa0059960:CDS
TTCAATTCAGGGAGAACCTT+CGG 0.411593 1_2:+17505439 Msa0059960:CDS
CAAATGTCAAAGAAGCAATC+AGG 0.416319 1_2:+17506332 Msa0059960:CDS
ACGATGAGGAAAACATGTTT+AGG 0.418481 1_2:+17505165 Msa0059960:CDS
AAAGTGAAGCAAATATTTGT+TGG 0.419876 1_2:-17503440 None:intergenic
ACGAAACATAAGTTTCAAAC+TGG 0.420279 1_2:-17504989 None:intergenic
CTCACATAAGGTGGCTTCCC+TGG 0.421499 1_2:-17505743 None:intergenic
GGACGACAGTTGAGCATTGA+TGG 0.424496 1_2:-17505911 None:intergenic
AGGCAGTTGGTTTCAATTCA+GGG 0.425383 1_2:+17505428 Msa0059960:CDS
ATAGAACGGTATGAAGTTGC+CGG 0.427124 1_2:+17506256 Msa0059960:CDS
ATTCATAGAGTAATTGCTTC+GGG 0.428091 1_2:-17505346 None:intergenic
TACCAGCTCAGAGCTAATTC+AGG 0.431218 1_2:+17504945 Msa0059960:CDS
TAAAGGCGTCGTTGAAGATT+CGG 0.440664 1_2:+17504486 Msa0059960:five_prime_UTR
TACAAGATAATAGAGGAAAT+GGG 0.441200 1_2:+17506382 Msa0059960:CDS
TCAACAAATCTTGGGAGAAA+GGG 0.445090 1_2:-17506094 None:intergenic
TCTATTATACTCTTTCGATA+AGG 0.451748 1_2:+17504536 Msa0059960:five_prime_UTR
GTTCACCACAAGAGCTTCCC+CGG 0.452377 1_2:-17505933 None:intergenic
TACCGTTGATACACTTTCAT+CGG 0.452419 1_2:-17505981 None:intergenic
ACCGTTGATACACTTTCATC+GGG 0.454201 1_2:-17505980 None:intergenic
GGGTTATATATTGACTTCCT+TGG 0.454975 1_2:-17505697 None:intergenic
TATAGGCTTCAACAAATCTT+GGG 0.456724 1_2:-17506102 None:intergenic
TTCAACAAATCTTGGGAGAA+AGG 0.466115 1_2:-17506095 None:intergenic
TATGTCATTGATTATATCTC+CGG 0.466200 1_2:+17504683 Msa0059960:exon
TTTCAACATTCAACCTATCC+TGG 0.466892 1_2:-17506177 None:intergenic
TCTGAGGACAAAGATTCCTT+TGG 0.467050 1_2:-17505266 None:intergenic
TGATAGAACCGTCTAGTTCT+GGG 0.467216 1_2:+17504883 Msa0059960:CDS
GAGTTGGCTGATGCGTCGTA+TGG 0.473339 1_2:+17504705 Msa0059960:CDS
TCAATGCTCAACTGTCGTCC+CGG 0.473427 1_2:+17505914 Msa0059960:CDS
CAATGCTCAACTGTCGTCCC+GGG 0.477152 1_2:+17505915 Msa0059960:CDS
CATTCATAGAGTAATTGCTT+CGG 0.479741 1_2:-17505347 None:intergenic
GCTTATCAGGAGCAAAATCA+TGG 0.483645 1_2:-17504782 None:intergenic
TCTTAACCATGCGGAGAAGC+TGG 0.488691 1_2:-17506015 None:intergenic
GTTGGAGGCCTAAGTATCAT+AGG 0.491231 1_2:+17505806 Msa0059960:CDS
CAGCTATGATCGATGATCTC+AGG 0.491997 1_2:+17505408 Msa0059960:CDS
GCCAAGCTATACTCGTTAAA+GGG 0.492765 1_2:+17504732 Msa0059960:CDS
TGAAAATACAAGAATTGGAG+AGG 0.493157 1_2:+17505108 Msa0059960:CDS
TCGTCCCGGGGAAGCTCTTG+TGG 0.495157 1_2:+17505928 Msa0059960:CDS
GTCTTTGACCTATGATACTT+AGG 0.498050 1_2:-17505814 None:intergenic
CGACTTGTTCTGAATGGGAT+TGG 0.499359 1_2:-17505231 None:intergenic
AAATCATGGGAGGACAAGCC+AGG 0.501458 1_2:-17504768 None:intergenic
AAGCAATCAGGGAACTCATA+AGG 0.502002 1_2:+17506344 Msa0059960:CDS
ATTGATTATATCTCCGGAGT+TGG 0.504145 1_2:+17504689 Msa0059960:CDS
TGTCATTGCGTGTGAGGGCG+AGG 0.504713 1_2:+17506228 Msa0059960:CDS
CCACCTTATGTGAGATTGAC+CGG 0.507424 1_2:+17505752 Msa0059960:CDS
CAAACACTTGCTTCTAGGCC+AGG 0.511442 1_2:+17505725 Msa0059960:CDS
TCCCTTTAACGAGTATAGCT+TGG 0.513672 1_2:-17504733 None:intergenic
TGATGCGACTTGTTCTGAAT+GGG 0.520418 1_2:-17505236 None:intergenic
AACCTATCCTGGCTCTCTCT+CGG 0.522464 1_2:-17506166 None:intergenic
CATCGGGCATGTGATGAAGC+TGG 0.525795 1_2:-17505964 None:intergenic
TTGTTATAATCTCTCTCACT+TGG 0.527054 1_2:+17503348 Msa0059960:five_prime_UTR
GGATAGAATTGCAGCCTACA+TGG 0.528367 1_2:+17505460 Msa0059960:CDS
ACACCGGTCAATCTCACATA+AGG 0.532296 1_2:-17505755 None:intergenic
AAACTTATGTTTCGTCTACA+AGG 0.534213 1_2:+17504997 Msa0059960:CDS
TTATAAAGCTTTGAAATGCA+AGG 0.539721 1_2:+17505526 Msa0059960:CDS
GGCTATTAAAGATGATAAGA+AGG 0.541700 1_2:+17505649 Msa0059960:CDS
GAAGAAGAAGAAAAGAGAGA+AGG 0.543082 1_2:-17503392 None:intergenic
AAATATCACAAAACTTTCCT+CGG 0.543588 1_2:+17505321 Msa0059960:CDS
GCTACGGCACGAAATTCAAA+TGG 0.544112 1_2:-17505866 None:intergenic
ATGATGCGACTTGTTCTGAA+TGG 0.544379 1_2:-17505237 None:intergenic
GTTTAGGACTGTTCAGAGCA+TGG 0.548249 1_2:+17505181 Msa0059960:CDS
TCCAAAACTTGTGACAGTTG+TGG 0.549059 1_2:+17506045 Msa0059960:CDS
GAATCCGTGCAACGATCTGT+TGG 0.549569 1_2:+17505788 Msa0059960:CDS
AGCAATGCAGATACTATTCG+AGG 0.550536 1_2:+17505574 Msa0059960:CDS
TGACAAGTACAAGATAATAG+AGG 0.550853 1_2:+17506375 Msa0059960:CDS
GTTCCTCTGTAGGTGAATCA+AGG 0.552374 1_2:-17504860 None:intergenic
AGGGCAAGGATGAAAATGGC+TGG 0.554676 1_2:+17506286 Msa0059960:CDS
TTGGCACACTCAGTAAAGTC+AGG 0.557975 1_2:-17505212 None:intergenic
AGAAGCAAGTGTTTGTATCA+GGG 0.558440 1_2:-17505717 None:intergenic
GAATGTTGAAAGGCAATGCT+TGG 0.559198 1_2:+17506189 Msa0059960:CDS
AAGGGAGCCGATGTTACACC+TGG 0.562237 1_2:+17504750 Msa0059960:CDS
CTCCTGAATTAGCTCTGAGC+TGG 0.562844 1_2:-17504947 None:intergenic
ACAAGATAATAGAGGAAATG+GGG 0.563828 1_2:+17506383 Msa0059960:CDS
GATCGATGATCTCAGGCAGT+TGG 0.564311 1_2:+17505415 Msa0059960:CDS
AGTTGGCTGATGCGTCGTAT+GGG 0.565392 1_2:+17504706 Msa0059960:CDS
AATTTCGTGCCGTAGCATCA+AGG 0.565456 1_2:+17505873 Msa0059960:CDS
TTGCCGGAAAATGGAGGGCA+AGG 0.570168 1_2:+17506272 Msa0059960:CDS
GAATTGGAGAGGGCACTGCT+TGG 0.573720 1_2:+17505119 Msa0059960:CDS
AATTGAAGACCTTGATGCTA+CGG 0.573876 1_2:-17505882 None:intergenic
TCCGTGCAACGATCTGTTGG+AGG 0.575063 1_2:+17505791 Msa0059960:CDS
CAACAAATCTTGGGAGAAAG+GGG 0.584291 1_2:-17506093 None:intergenic
GAAAAGCTAGCAGAAGTACT+CGG 0.588604 1_2:+17505839 Msa0059960:CDS
ATGAAGTTGCCGGAAAATGG+AGG 0.588856 1_2:+17506266 Msa0059960:CDS
AGTAATTGCTTCGGGGTCCG+AGG 0.588857 1_2:-17505338 None:intergenic
CGTCAAATTCAAAGCAATCA+GGG 0.590617 1_2:-17505073 None:intergenic
CTGGCTCTCTCTCGGAAGAG+TGG 0.590772 1_2:-17506158 None:intergenic
GTCAATGTCATTGCGTGTGA+GGG 0.592691 1_2:+17506223 Msa0059960:CDS
AAATGTCAAAGAAGCAATCA+GGG 0.593878 1_2:+17506333 Msa0059960:CDS
GGAAATTCTGTTCATGCTGA+TGG 0.594308 1_2:+17504912 Msa0059960:CDS
GCCTCCAACAGATCGTTGCA+CGG 0.595803 1_2:-17505792 None:intergenic
GCCCGATGAAAGTGTATCAA+CGG 0.597244 1_2:+17505979 Msa0059960:CDS
TAAATCCAAACTTAAAACAT+GGG 0.600368 1_2:-17505601 None:intergenic
ATAAATCCAAACTTAAAACA+TGG 0.605905 1_2:-17505602 None:intergenic
ACGAGACCAGCTTCTCCGCA+TGG 0.606476 1_2:+17506009 Msa0059960:CDS
GGTTCTATCAGTTCCTCTGT+AGG 0.608223 1_2:-17504870 None:intergenic
TTGAAAGGCAATGCTTGGCG+AGG 0.610581 1_2:+17506194 Msa0059960:CDS
AGGATAGGTTGAATGTTGAA+AGG 0.611055 1_2:+17506179 Msa0059960:CDS
CCTGATATCCCAGAACTAGA+CGG 0.611349 1_2:-17504891 None:intergenic
TGTGCTAGCGCACTTTCAGA+AGG 0.613130 1_2:+17505368 Msa0059960:CDS
CTTCCTTGATTCACCTACAG+AGG 0.616392 1_2:+17504857 Msa0059960:CDS
AAACACTTGCTTCTAGGCCA+GGG 0.617893 1_2:+17505726 Msa0059960:CDS
ATATTGATAATATATGAATG+AGG 0.622400 1_2:+17503320 Msa0059960:five_prime_UTR
GCTACTAAGATTTGAATCTG+AGG 0.622598 1_2:-17505282 None:intergenic
TAGGCTGCAATTCTATCCGA+AGG 0.623160 1_2:-17505455 None:intergenic
GTCGCATCATGACTCGCCAA+AGG 0.623457 1_2:+17505250 Msa0059960:CDS
CCGGTCAATCTCACATAAGG+TGG 0.626350 1_2:-17505752 None:intergenic
ATTGCAGCCTACATGGTGGA+AGG 0.627921 1_2:+17505467 Msa0059960:CDS
CAAGATAATAGAGGAAATGG+GGG 0.629220 1_2:+17506384 Msa0059960:CDS
TGAAAGGCAATGCTTGGCGA+GGG 0.630756 1_2:+17506195 Msa0059960:CDS
TACGACGCATCAGCCAACTC+CGG 0.632209 1_2:-17504702 None:intergenic
GAAAATACAAGAATTGGAGA+GGG 0.632436 1_2:+17505109 Msa0059960:CDS
TGTCAATGTCATTGCGTGTG+AGG 0.632541 1_2:+17506222 Msa0059960:CDS
TTCCGAGAGAGAGCCAGGAT+AGG 0.634206 1_2:+17506164 Msa0059960:CDS
CTTATCAGGAGCAAAATCAT+GGG 0.634286 1_2:-17504781 None:intergenic
TGAAGTTGCCGGAAAATGGA+GGG 0.635160 1_2:+17506267 Msa0059960:CDS
GCTGCTAGACGATCAGAAGA+CGG 0.639186 1_2:-17505554 None:intergenic
CTACATGGTGGAAGGACTTG+CGG 0.640770 1_2:+17505475 Msa0059960:CDS
CGCAAGTCCTTCCACCATGT+AGG 0.643612 1_2:-17505474 None:intergenic
GGACAAGCCAGGTGTAACAT+CGG 0.651711 1_2:-17504757 None:intergenic
TTCACCACAAGAGCTTCCCC+GGG 0.654911 1_2:-17505932 None:intergenic
TGCTAATGGTGCTATCTCTG+AGG 0.658374 1_2:+17505628 Msa0059960:CDS
AACAAATCTTGGGAGAAAGG+GGG 0.658411 1_2:-17506092 None:intergenic
ATCAGGAGCAAAATCATGGG+AGG 0.665372 1_2:-17504778 None:intergenic
CAGCTCAGAGCTAATTCAGG+AGG 0.666948 1_2:+17504948 Msa0059960:CDS
TTCATAGAGTAATTGCTTCG+GGG 0.668763 1_2:-17505345 None:intergenic
AATGCTCAACTGTCGTCCCG+GGG 0.671564 1_2:+17505916 Msa0059960:CDS
GATTAAGGCTCTTAACCATG+CGG 0.671983 1_2:-17506024 None:intergenic
CGGCTCGTTTGGCTTCATCG+GGG 0.673846 1_2:+17505495 Msa0059960:CDS
TGATGAGTGTGATGACGATG+AGG 0.676899 1_2:+17505151 Msa0059960:CDS
GAGATCATCGATCATAGCTG+AGG 0.677523 1_2:-17505405 None:intergenic
TAGAATTGCAGCCTACATGG+TGG 0.682524 1_2:+17505463 Msa0059960:CDS
AGGGCGAGGATAGAATAGAA+CGG 0.705742 1_2:+17506242 Msa0059960:CDS
AAGTGAGAGAGATTATAACA+AGG 0.732597 1_2:-17503346 None:intergenic
AAGATAATAGAGGAAATGGG+GGG 0.732816 1_2:+17506385 Msa0059960:CDS
GGATTCGGGATCATCAACAC+CGG 0.740769 1_2:-17505771 None:intergenic
GCTAGACGATCAGAAGACGG+AGG 0.750067 1_2:-17505551 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTCAAAAAATTTCATT+TGG + chr1_2:17503998-17504017 Msa0059960:intron 10.0%
!!! TCAAATAAATTTTATCAAAA+AGG + chr1_2:17504167-17504186 Msa0059960:intron 10.0%
!!! AAAATTGCATATTTTTAAAT+AGG + chr1_2:17507323-17507342 Msa0059960:intron 10.0%
!! TTTATAAACAAAAATAGAAA+TGG + chr1_2:17507379-17507398 Msa0059960:intron 10.0%
!!! ATATTGATAATATATGAATG+AGG + chr1_2:17503320-17503339 Msa0059960:five_prime_UTR 15.0%
!!! AATTTTGGTTAAAACATTAA+TGG - chr1_2:17504146-17504165 None:intergenic 15.0%
!! TTTAGTCTATTTATGTATTT+AGG + chr1_2:17506803-17506822 Msa0059960:intron 15.0%
!! TGTATATTAGTAGAAAAATA+TGG + chr1_2:17507283-17507302 Msa0059960:intron 15.0%
!!! AAAACTTGTTGTAATTAAGA+AGG - chr1_2:17503576-17503595 None:intergenic 20.0%
!!! TTCTTAATTACAACAAGTTT+TGG + chr1_2:17503575-17503594 Msa0059960:intron 20.0%
!! TATGTAGAAGAAAAAAAACT+TGG + chr1_2:17504559-17504578 Msa0059960:intron 20.0%
!! AAGAAAAAAAACTTGGTTAA+TGG + chr1_2:17504566-17504585 Msa0059960:intron 20.0%
!!! AAAGTTTTGTGATATTTCTT+TGG - chr1_2:17505318-17505337 None:intergenic 20.0%
!! TAAATCCAAACTTAAAACAT+GGG - chr1_2:17505604-17505623 None:intergenic 20.0%
!! ATAAATCCAAACTTAAAACA+TGG - chr1_2:17505605-17505624 None:intergenic 20.0%
!! ACTATTTACTCTAACAAAAA+GGG - chr1_2:17506475-17506494 None:intergenic 20.0%
!!! TTGTAATTTTCAAACATTCA+TGG - chr1_2:17506536-17506555 None:intergenic 20.0%
!! AACTAATAAAAAACACATTC+AGG - chr1_2:17506566-17506585 None:intergenic 20.0%
!!! TTTTTGTTATATAGACTCTT+TGG + chr1_2:17506765-17506784 Msa0059960:intron 20.0%
!!! TTTTGTTATATAGACTCTTT+GGG + chr1_2:17506766-17506785 Msa0059960:intron 20.0%
!! TCAAAAGAAATGCAAAATTA+TGG - chr1_2:17506971-17506990 None:intergenic 20.0%
!! AACTTCAATTATGATCAAAT+AGG - chr1_2:17507490-17507509 None:intergenic 20.0%
! AAAGTGAAGCAAATATTTGT+TGG - chr1_2:17503443-17503462 None:intergenic 25.0%
!!! CATGTTTTTGTGTTTAACAT+AGG - chr1_2:17503737-17503756 None:intergenic 25.0%
! CTATGTTAAACACAAAAACA+TGG + chr1_2:17503735-17503754 Msa0059960:intron 25.0%
!!! AGAAATTCATCATTGTTTTG+TGG + chr1_2:17503892-17503911 Msa0059960:intron 25.0%
! TTTCATTTGGTTAAGACAAT+TGG + chr1_2:17504011-17504030 Msa0059960:intron 25.0%
! CTGTCATTCTTTATATAGAT+CGG + chr1_2:17504400-17504419 Msa0059960:intron 25.0%
! TCTATTATACTCTTTCGATA+AGG + chr1_2:17504536-17504555 Msa0059960:five_prime_UTR 25.0%
!! TATGTCATTGATTATATCTC+CGG + chr1_2:17504683-17504702 Msa0059960:exon 25.0%
! CAAGAAATCTGATTCAAAAT+TGG - chr1_2:17505042-17505061 None:intergenic 25.0%
!! CAATTTTGAATCAGATTTCT+TGG + chr1_2:17505040-17505059 Msa0059960:CDS 25.0%
! AAATATCACAAAACTTTCCT+CGG + chr1_2:17505321-17505340 Msa0059960:CDS 25.0%
!!! TTATAAAGCTTTGAAATGCA+AGG + chr1_2:17505526-17505545 Msa0059960:CDS 25.0%
!!! ATAGATTTTGATATCAACCA+AGG + chr1_2:17505680-17505699 Msa0059960:CDS 25.0%
! TACAAGATAATAGAGGAAAT+GGG + chr1_2:17506382-17506401 Msa0059960:CDS 25.0%
! GACTATTTACTCTAACAAAA+AGG - chr1_2:17506476-17506495 None:intergenic 25.0%
!! GTTAGAGTAAATAGTCATTT+TGG + chr1_2:17506480-17506499 Msa0059960:intron 25.0%
! ATAAAAAACACATTCAGGTA+TGG - chr1_2:17506561-17506580 None:intergenic 25.0%
!! TGTCTTCTGTTAGTAATTTT+GGG - chr1_2:17506662-17506681 None:intergenic 25.0%
!! ATGTCTTCTGTTAGTAATTT+TGG - chr1_2:17506663-17506682 None:intergenic 25.0%
! TTGTAATTTCGATAGTTTCA+AGG + chr1_2:17506695-17506714 Msa0059960:intron 25.0%
! TCTATTTATGTATTTAGGTG+TGG + chr1_2:17506808-17506827 Msa0059960:intron 25.0%
! TCAACTGTGTTATGTATTAT+GGG - chr1_2:17507120-17507139 None:intergenic 25.0%
! TTAAGCTCAAAATTATGCAT+TGG - chr1_2:17507157-17507176 None:intergenic 25.0%
AAGTGAGAGAGATTATAACA+AGG - chr1_2:17503349-17503368 None:intergenic 30.0%
TTGTTATAATCTCTCTCACT+TGG + chr1_2:17503348-17503367 Msa0059960:five_prime_UTR 30.0%
! AGCAAATATTTGTTGGTAGA+AGG - chr1_2:17503436-17503455 None:intergenic 30.0%
!!! AAGTTTTGGATTCTTCAACA+AGG + chr1_2:17503589-17503608 Msa0059960:intron 30.0%
AAACACAAAAACATGGTCAT+AGG + chr1_2:17503742-17503761 Msa0059960:intron 30.0%
! GGTTAAAACATTAATGGACA+TGG - chr1_2:17504140-17504159 None:intergenic 30.0%
TGTTTATCCTATCTCAGTAA+AGG + chr1_2:17504469-17504488 Msa0059960:intron 30.0%
ACGAAACATAAGTTTCAAAC+TGG - chr1_2:17504992-17505011 None:intergenic 30.0%
AAACTTATGTTTCGTCTACA+AGG + chr1_2:17504997-17505016 Msa0059960:CDS 30.0%
GCGATTGAAAATACAAGAAT+TGG + chr1_2:17505103-17505122 Msa0059960:CDS 30.0%
TGAAAATACAAGAATTGGAG+AGG + chr1_2:17505108-17505127 Msa0059960:CDS 30.0%
GAAAATACAAGAATTGGAGA+GGG + chr1_2:17505109-17505128 Msa0059960:CDS 30.0%
ATTCATAGAGTAATTGCTTC+GGG - chr1_2:17505349-17505368 None:intergenic 30.0%
CATTCATAGAGTAATTGCTT+CGG - chr1_2:17505350-17505369 None:intergenic 30.0%
TTTGGATTTATTGCTGCTAA+TGG + chr1_2:17505614-17505633 Msa0059960:CDS 30.0%
GGCTATTAAAGATGATAAGA+AGG + chr1_2:17505649-17505668 Msa0059960:CDS 30.0%
TATAGGCTTCAACAAATCTT+GGG - chr1_2:17506105-17506124 None:intergenic 30.0%
TTATAGGCTTCAACAAATCT+TGG - chr1_2:17506106-17506125 None:intergenic 30.0%
AAATGTCAAAGAAGCAATCA+GGG + chr1_2:17506333-17506352 Msa0059960:CDS 30.0%
TGACAAGTACAAGATAATAG+AGG + chr1_2:17506375-17506394 Msa0059960:CDS 30.0%
GTACAAGATAATAGAGGAAA+TGG + chr1_2:17506381-17506400 Msa0059960:CDS 30.0%
ACAAGATAATAGAGGAAATG+GGG + chr1_2:17506383-17506402 Msa0059960:CDS 30.0%
ACTAACAAAAGACACATTTG+AGG - chr1_2:17506629-17506648 None:intergenic 30.0%
! GTGTCTTTTGTTAGTCAATT+TGG + chr1_2:17506634-17506653 Msa0059960:intron 30.0%
! GTCTTCTGTTAGTAATTTTG+GGG - chr1_2:17506661-17506680 None:intergenic 30.0%
!! AAGAAGTGAAGAGTCATTTT+TGG - chr1_2:17506747-17506766 None:intergenic 30.0%
ATATAGACTCTTTGGGTATT+TGG + chr1_2:17506773-17506792 Msa0059960:intron 30.0%
TACATCTGATATGGATATTC+TGG - chr1_2:17506835-17506854 None:intergenic 30.0%
ACGTTAGTATACATCTGATA+TGG - chr1_2:17506844-17506863 None:intergenic 30.0%
AACATAAAACAGCTATGCTT+AGG + chr1_2:17506915-17506934 Msa0059960:intron 30.0%
GTGATAGTTGTATCTAACTT+AGG + chr1_2:17507020-17507039 Msa0059960:intron 30.0%
GTCAACTGTGTTATGTATTA+TGG - chr1_2:17507121-17507140 None:intergenic 30.0%
TTTAAATAGGTCGTCTATAG+CGG + chr1_2:17507336-17507355 Msa0059960:intron 30.0%
!! CAATATTGACTCAACTAATC+TGG - chr1_2:17507415-17507434 None:intergenic 30.0%
! AGTTGAGTCAATATTGACTA+TGG + chr1_2:17507419-17507438 Msa0059960:intron 30.0%
GAAGAAGAAGAAAAGAGAGA+AGG - chr1_2:17503395-17503414 None:intergenic 35.0%
!! CATTTCTAGCTACATCATTC+AGG + chr1_2:17503466-17503485 Msa0059960:five_prime_UTR 35.0%
TAACATAGGACGAATCTACA+TGG - chr1_2:17503723-17503742 None:intergenic 35.0%
!!! TTCGCAGCAGTTATTTTTTC+TGG + chr1_2:17503817-17503836 Msa0059960:intron 35.0%
TATGCTCAAAATCTTAGTGC+AGG + chr1_2:17504246-17504265 Msa0059960:intron 35.0%
ACGACATTAGAAGTTCCTTT+CGG - chr1_2:17504439-17504458 None:intergenic 35.0%
ATTGATTATATCTCCGGAGT+TGG + chr1_2:17504689-17504708 Msa0059960:CDS 35.0%
CTTATCAGGAGCAAAATCAT+GGG - chr1_2:17504784-17504803 None:intergenic 35.0%
TACATGCTTCTATGCTTATC+AGG - chr1_2:17504798-17504817 None:intergenic 35.0%
CGTCAAATTCAAAGCAATCA+GGG - chr1_2:17505076-17505095 None:intergenic 35.0%
! CCTGATTGCTTTGAATTTGA+CGG + chr1_2:17505074-17505093 Msa0059960:CDS 35.0%
ACGATGAGGAAAACATGTTT+AGG + chr1_2:17505165-17505184 Msa0059960:CDS 35.0%
GCTACTAAGATTTGAATCTG+AGG - chr1_2:17505285-17505304 None:intergenic 35.0%
!! TGATATTTCTTTGGTGCTAC+TGG - chr1_2:17505309-17505328 None:intergenic 35.0%
TTCATAGAGTAATTGCTTCG+GGG - chr1_2:17505348-17505367 None:intergenic 35.0%
!! GGGTTATATATTGACTTCCT+TGG - chr1_2:17505700-17505719 None:intergenic 35.0%
AGAAGCAAGTGTTTGTATCA+GGG - chr1_2:17505720-17505739 None:intergenic 35.0%
TAGAAGCAAGTGTTTGTATC+AGG - chr1_2:17505721-17505740 None:intergenic 35.0%
TGATACAAACACTTGCTTCT+AGG + chr1_2:17505720-17505739 Msa0059960:CDS 35.0%
GTCTTTGACCTATGATACTT+AGG - chr1_2:17505817-17505836 None:intergenic 35.0%
AATTGAAGACCTTGATGCTA+CGG - chr1_2:17505885-17505904 None:intergenic 35.0%
TACCGTTGATACACTTTCAT+CGG - chr1_2:17505984-17506003 None:intergenic 35.0%
!!! CTGTCACAAGTTTTGGATTA+AGG - chr1_2:17506042-17506061 None:intergenic 35.0%
TCAACAAATCTTGGGAGAAA+GGG - chr1_2:17506097-17506116 None:intergenic 35.0%
TTCAACAAATCTTGGGAGAA+AGG - chr1_2:17506098-17506117 None:intergenic 35.0%
CACAGCAGAGTAGTAATTAT+AGG - chr1_2:17506122-17506141 None:intergenic 35.0%
TTTCAACATTCAACCTATCC+TGG - chr1_2:17506180-17506199 None:intergenic 35.0%
! AGGATAGGTTGAATGTTGAA+AGG + chr1_2:17506179-17506198 Msa0059960:CDS 35.0%
TCTTTGACATTTGTGCTCAT+TGG - chr1_2:17506325-17506344 None:intergenic 35.0%
CAAATGTCAAAGAAGCAATC+AGG + chr1_2:17506332-17506351 Msa0059960:CDS 35.0%
CAAGATAATAGAGGAAATGG+GGG + chr1_2:17506384-17506403 Msa0059960:CDS 35.0%
AAGATAATAGAGGAAATGGG+GGG + chr1_2:17506385-17506404 Msa0059960:CDS 35.0%
GTATCTAACTTAGGTGTGTT+TGG + chr1_2:17507029-17507048 Msa0059960:intron 35.0%
CTGTGTTATGTATTATGGGA+CGG - chr1_2:17507116-17507135 None:intergenic 35.0%
! ATGCATTGGTATGCGTAATA+AGG - chr1_2:17507143-17507162 None:intergenic 35.0%
ATTCATCGAGACGTATCTAA+CGG + chr1_2:17507235-17507254 Msa0059960:intron 35.0%
GTAGAAGGAAGCTGAGAAAA+TGG - chr1_2:17503421-17503440 None:intergenic 40.0%
ATACACATGTCTCTACACTC+TGG - chr1_2:17504058-17504077 None:intergenic 40.0%
GGTTCTTTGTTCTAACCGAA+AGG + chr1_2:17504421-17504440 Msa0059960:intron 40.0%
!! TAAAGGCGTCGTTGAAGATT+CGG + chr1_2:17504486-17504505 Msa0059960:five_prime_UTR 40.0%
TGCCAAGCTATACTCGTTAA+AGG + chr1_2:17504731-17504750 Msa0059960:CDS 40.0%
GCCAAGCTATACTCGTTAAA+GGG + chr1_2:17504732-17504751 Msa0059960:CDS 40.0%
TCCCTTTAACGAGTATAGCT+TGG - chr1_2:17504736-17504755 None:intergenic 40.0%
GCTTATCAGGAGCAAAATCA+TGG - chr1_2:17504785-17504804 None:intergenic 40.0%
!! TGATAGAACCGTCTAGTTCT+GGG + chr1_2:17504883-17504902 Msa0059960:CDS 40.0%
GGAAATTCTGTTCATGCTGA+TGG + chr1_2:17504912-17504931 Msa0059960:CDS 40.0%
AGCTAATTCAGGAGGTTCTA+TGG + chr1_2:17504956-17504975 Msa0059960:CDS 40.0%
CCGTCAAATTCAAAGCAATC+AGG - chr1_2:17505077-17505096 None:intergenic 40.0%
! TGATGCGACTTGTTCTGAAT+GGG - chr1_2:17505239-17505258 None:intergenic 40.0%
! ATGATGCGACTTGTTCTGAA+TGG - chr1_2:17505240-17505259 None:intergenic 40.0%
TCTGAGGACAAAGATTCCTT+TGG - chr1_2:17505269-17505288 None:intergenic 40.0%
! AGGCAGTTGGTTTCAATTCA+GGG + chr1_2:17505428-17505447 Msa0059960:CDS 40.0%
TTCAATTCAGGGAGAACCTT+CGG + chr1_2:17505439-17505458 Msa0059960:CDS 40.0%
AGCAATGCAGATACTATTCG+AGG + chr1_2:17505574-17505593 Msa0059960:CDS 40.0%
!!! GTGTGCCCATGTTTTAAGTT+TGG + chr1_2:17505596-17505615 Msa0059960:CDS 40.0%
!! GAAAAGCTAGCAGAAGTACT+CGG + chr1_2:17505839-17505858 Msa0059960:CDS 40.0%
ACCGTTGATACACTTTCATC+GGG - chr1_2:17505983-17506002 None:intergenic 40.0%
GATTAAGGCTCTTAACCATG+CGG - chr1_2:17506027-17506046 None:intergenic 40.0%
TCCAAAACTTGTGACAGTTG+TGG + chr1_2:17506045-17506064 Msa0059960:CDS 40.0%
! TCCACAACTGTCACAAGTTT+TGG - chr1_2:17506049-17506068 None:intergenic 40.0%
AACAAATCTTGGGAGAAAGG+GGG - chr1_2:17506095-17506114 None:intergenic 40.0%
CAACAAATCTTGGGAGAAAG+GGG - chr1_2:17506096-17506115 None:intergenic 40.0%
GAATGTTGAAAGGCAATGCT+TGG + chr1_2:17506189-17506208 Msa0059960:CDS 40.0%
ATAGAACGGTATGAAGTTGC+CGG + chr1_2:17506256-17506275 Msa0059960:CDS 40.0%
AAGCAATCAGGGAACTCATA+AGG + chr1_2:17506344-17506363 Msa0059960:CDS 40.0%
! AAATGGGGGGACTTCATTTT+GGG + chr1_2:17506398-17506417 Msa0059960:CDS 40.0%
! ACTTGATTGTTGCTTCAGCA+TGG + chr1_2:17506434-17506453 Msa0059960:intron 40.0%
GTTATGTATTATGGGACGGA+GGG - chr1_2:17507112-17507131 None:intergenic 40.0%
TGTTATGTATTATGGGACGG+AGG - chr1_2:17507113-17507132 None:intergenic 40.0%
! TACACTCTGGAGATACTAGC+TGG - chr1_2:17504045-17504064 None:intergenic 45.0%
AACGACGCCTTTACTGAGAT+AGG - chr1_2:17504479-17504498 None:intergenic 45.0%
ATCAGGAGCAAAATCATGGG+AGG - chr1_2:17504781-17504800 None:intergenic 45.0%
CTTCCTTGATTCACCTACAG+AGG + chr1_2:17504857-17504876 Msa0059960:CDS 45.0%
GTTCCTCTGTAGGTGAATCA+AGG - chr1_2:17504863-17504882 None:intergenic 45.0%
GGTTCTATCAGTTCCTCTGT+AGG - chr1_2:17504873-17504892 None:intergenic 45.0%
! CTGATAGAACCGTCTAGTTC+TGG + chr1_2:17504882-17504901 Msa0059960:CDS 45.0%
CCTGATATCCCAGAACTAGA+CGG - chr1_2:17504894-17504913 None:intergenic 45.0%
TACCAGCTCAGAGCTAATTC+AGG + chr1_2:17504945-17504964 Msa0059960:CDS 45.0%
! TGATGAGTGTGATGACGATG+AGG + chr1_2:17505151-17505170 Msa0059960:CDS 45.0%
! GTTTAGGACTGTTCAGAGCA+TGG + chr1_2:17505181-17505200 Msa0059960:CDS 45.0%
TTGGCACACTCAGTAAAGTC+AGG - chr1_2:17505215-17505234 None:intergenic 45.0%
! CGACTTGTTCTGAATGGGAT+TGG - chr1_2:17505234-17505253 None:intergenic 45.0%
GAGATCATCGATCATAGCTG+AGG - chr1_2:17505408-17505427 None:intergenic 45.0%
CAGCTATGATCGATGATCTC+AGG + chr1_2:17505408-17505427 Msa0059960:CDS 45.0%
! CAGGCAGTTGGTTTCAATTC+AGG + chr1_2:17505427-17505446 Msa0059960:CDS 45.0%
TAGGCTGCAATTCTATCCGA+AGG - chr1_2:17505458-17505477 None:intergenic 45.0%
GGATAGAATTGCAGCCTACA+TGG + chr1_2:17505460-17505479 Msa0059960:CDS 45.0%
TAGAATTGCAGCCTACATGG+TGG + chr1_2:17505463-17505482 Msa0059960:CDS 45.0%
!! TGCTAATGGTGCTATCTCTG+AGG + chr1_2:17505628-17505647 Msa0059960:CDS 45.0%
AAACACTTGCTTCTAGGCCA+GGG + chr1_2:17505726-17505745 Msa0059960:CDS 45.0%
CCACCTTATGTGAGATTGAC+CGG + chr1_2:17505752-17505771 Msa0059960:CDS 45.0%
ACACCGGTCAATCTCACATA+AGG - chr1_2:17505758-17505777 None:intergenic 45.0%
AACAGATCGTTGCACGGATT+CGG - chr1_2:17505789-17505808 None:intergenic 45.0%
GTTGGAGGCCTAAGTATCAT+AGG + chr1_2:17505806-17505825 Msa0059960:CDS 45.0%
GCTACGGCACGAAATTCAAA+TGG - chr1_2:17505869-17505888 None:intergenic 45.0%
AATTTCGTGCCGTAGCATCA+AGG + chr1_2:17505873-17505892 Msa0059960:CDS 45.0%
! GCCCGATGAAAGTGTATCAA+CGG + chr1_2:17505979-17505998 Msa0059960:CDS 45.0%
TGTCAATGTCATTGCGTGTG+AGG + chr1_2:17506222-17506241 Msa0059960:CDS 45.0%
GTCAATGTCATTGCGTGTGA+GGG + chr1_2:17506223-17506242 Msa0059960:CDS 45.0%
AGGGCGAGGATAGAATAGAA+CGG + chr1_2:17506242-17506261 Msa0059960:CDS 45.0%
GGTATGAAGTTGCCGGAAAA+TGG + chr1_2:17506263-17506282 Msa0059960:CDS 45.0%
ATGAAGTTGCCGGAAAATGG+AGG + chr1_2:17506266-17506285 Msa0059960:CDS 45.0%
TGAAGTTGCCGGAAAATGGA+GGG + chr1_2:17506267-17506286 Msa0059960:CDS 45.0%
ATGGAGGGCAAGGATGAAAA+TGG + chr1_2:17506282-17506301 Msa0059960:CDS 45.0%
! GAAATGGGGGGACTTCATTT+TGG + chr1_2:17506397-17506416 Msa0059960:CDS 45.0%
GTGACAATGACTCGCATTCA+AGG - chr1_2:17506507-17506526 None:intergenic 45.0%
! ATAGGTCGTCTATAGCGGAA+TGG + chr1_2:17507341-17507360 Msa0059960:intron 45.0%
!! AAAAAAAAAAAAATACTAAA+AGG - chr1_2:17503626-17503645 None:intergenic 5.0%
!!! TTTTAGTATTTTTTTTTTTT+TGG + chr1_2:17503625-17503644 Msa0059960:intron 5.0%
!!! TAAAATTTATTTGATAATTT+TGG - chr1_2:17504161-17504180 None:intergenic 5.0%
AGTTGGCTGATGCGTCGTAT+GGG + chr1_2:17504706-17504725 Msa0059960:CDS 50.0%
GGACAAGCCAGGTGTAACAT+CGG - chr1_2:17504760-17504779 None:intergenic 50.0%
AAATCATGGGAGGACAAGCC+AGG - chr1_2:17504771-17504790 None:intergenic 50.0%
!! CCGTCTAGTTCTGGGATATC+AGG + chr1_2:17504891-17504910 Msa0059960:CDS 50.0%
!! CTCCTGAATTAGCTCTGAGC+TGG - chr1_2:17504950-17504969 None:intergenic 50.0%
CAGCTCAGAGCTAATTCAGG+AGG + chr1_2:17504948-17504967 Msa0059960:CDS 50.0%
TGTGCTAGCGCACTTTCAGA+AGG + chr1_2:17505368-17505387 Msa0059960:CDS 50.0%
GATCGATGATCTCAGGCAGT+TGG + chr1_2:17505415-17505434 Msa0059960:CDS 50.0%
! ATTGCAGCCTACATGGTGGA+AGG + chr1_2:17505467-17505486 Msa0059960:CDS 50.0%
! CTACATGGTGGAAGGACTTG+CGG + chr1_2:17505475-17505494 Msa0059960:CDS 50.0%
GCTGCTAGACGATCAGAAGA+CGG - chr1_2:17505557-17505576 None:intergenic 50.0%
CAAACACTTGCTTCTAGGCC+AGG + chr1_2:17505725-17505744 Msa0059960:CDS 50.0%
CCGGTCAATCTCACATAAGG+TGG - chr1_2:17505755-17505774 None:intergenic 50.0%
GGATTCGGGATCATCAACAC+CGG - chr1_2:17505774-17505793 None:intergenic 50.0%
ACAGATCGTTGCACGGATTC+GGG - chr1_2:17505788-17505807 None:intergenic 50.0%
GAATCCGTGCAACGATCTGT+TGG + chr1_2:17505788-17505807 Msa0059960:CDS 50.0%
!! GGACGACAGTTGAGCATTGA+TGG - chr1_2:17505914-17505933 None:intergenic 50.0%
TCAATGCTCAACTGTCGTCC+CGG + chr1_2:17505914-17505933 Msa0059960:CDS 50.0%
TCTTAACCATGCGGAGAAGC+TGG - chr1_2:17506018-17506037 None:intergenic 50.0%
! AACCTATCCTGGCTCTCTCT+CGG - chr1_2:17506169-17506188 None:intergenic 50.0%
TTGAAAGGCAATGCTTGGCG+AGG + chr1_2:17506194-17506213 Msa0059960:CDS 50.0%
TGAAAGGCAATGCTTGGCGA+GGG + chr1_2:17506195-17506214 Msa0059960:CDS 50.0%
! CATCCTTGCCCTCCATTTTC+CGG - chr1_2:17506278-17506297 None:intergenic 50.0%
AGGGCAAGGATGAAAATGGC+TGG + chr1_2:17506286-17506305 Msa0059960:CDS 50.0%
! GTCGTCTATAGCGGAATGGA+GGG + chr1_2:17507345-17507364 Msa0059960:intron 50.0%
TACGACGCATCAGCCAACTC+CGG - chr1_2:17504705-17504724 None:intergenic 55.0%
GAGTTGGCTGATGCGTCGTA+TGG + chr1_2:17504705-17504724 Msa0059960:CDS 55.0%
AAGGGAGCCGATGTTACACC+TGG + chr1_2:17504750-17504769 Msa0059960:CDS 55.0%
!! GAATTGGAGAGGGCACTGCT+TGG + chr1_2:17505119-17505138 Msa0059960:CDS 55.0%
GTCGCATCATGACTCGCCAA+AGG + chr1_2:17505250-17505269 Msa0059960:CDS 55.0%
AGTAATTGCTTCGGGGTCCG+AGG - chr1_2:17505341-17505360 None:intergenic 55.0%
CGCAAGTCCTTCCACCATGT+AGG - chr1_2:17505477-17505496 None:intergenic 55.0%
! TGCGGCTCGTTTGGCTTCAT+CGG + chr1_2:17505493-17505512 Msa0059960:CDS 55.0%
GCTAGACGATCAGAAGACGG+AGG - chr1_2:17505554-17505573 None:intergenic 55.0%
CTCACATAAGGTGGCTTCCC+TGG - chr1_2:17505746-17505765 None:intergenic 55.0%
TCCGTGCAACGATCTGTTGG+AGG + chr1_2:17505791-17505810 Msa0059960:CDS 55.0%
GCCTCCAACAGATCGTTGCA+CGG - chr1_2:17505795-17505814 None:intergenic 55.0%
CAATGCTCAACTGTCGTCCC+GGG + chr1_2:17505915-17505934 Msa0059960:CDS 55.0%
AATGCTCAACTGTCGTCCCG+GGG + chr1_2:17505916-17505935 Msa0059960:CDS 55.0%
TTCACCACAAGAGCTTCCCC+GGG - chr1_2:17505935-17505954 None:intergenic 55.0%
GTTCACCACAAGAGCTTCCC+CGG - chr1_2:17505936-17505955 None:intergenic 55.0%
CATCGGGCATGTGATGAAGC+TGG - chr1_2:17505967-17505986 None:intergenic 55.0%
! TGGGAGAAAGGGGGAAGTGT+TGG - chr1_2:17506086-17506105 None:intergenic 55.0%
TTCCGAGAGAGAGCCAGGAT+AGG + chr1_2:17506164-17506183 Msa0059960:CDS 55.0%
TTGCCGGAAAATGGAGGGCA+AGG + chr1_2:17506272-17506291 Msa0059960:CDS 55.0%
! TGGGGGGACTTCATTTTGGG+TGG + chr1_2:17506401-17506420 Msa0059960:intron 55.0%
!! GGGGGGACTTCATTTTGGGT+GGG + chr1_2:17506402-17506421 Msa0059960:intron 55.0%
! GGTCGTCTATAGCGGAATGG+AGG + chr1_2:17507344-17507363 Msa0059960:intron 55.0%
GGAAGGACTTGCGGCTCGTT+TGG + chr1_2:17505484-17505503 Msa0059960:CDS 60.0%
! GCGGCTCGTTTGGCTTCATC+GGG + chr1_2:17505494-17505513 Msa0059960:CDS 60.0%
! CGGCTCGTTTGGCTTCATCG+GGG + chr1_2:17505495-17505514 Msa0059960:CDS 60.0%
ACGAGACCAGCTTCTCCGCA+TGG + chr1_2:17506009-17506028 Msa0059960:CDS 60.0%
! CTGGCTCTCTCTCGGAAGAG+TGG - chr1_2:17506161-17506180 None:intergenic 60.0%
CACTCTTCCGAGAGAGAGCC+AGG + chr1_2:17506159-17506178 Msa0059960:CDS 60.0%
TGTCATTGCGTGTGAGGGCG+AGG + chr1_2:17506228-17506247 Msa0059960:CDS 60.0%
TCGTCCCGGGGAAGCTCTTG+TGG + chr1_2:17505928-17505947 Msa0059960:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 17503315 17507575 17503315 ID=Msa0059960;Name=Msa0059960
chr1_2 mRNA 17503315 17507575 17503315 ID=Msa0059960-mRNA-1;Parent=Msa0059960;Name=Msa0059960-mRNA-1;_AED=0.12;_eAED=0.12;_QI=255|0.33|0.5|0.75|0.33|0.25|4|0|588
chr1_2 exon 17503315 17503487 17503315 ID=Msa0059960-mRNA-1:exon:6766;Parent=Msa0059960-mRNA-1
chr1_2 exon 17504486 17504557 17504486 ID=Msa0059960-mRNA-1:exon:6767;Parent=Msa0059960-mRNA-1
chr1_2 exon 17504674 17506419 17504674 ID=Msa0059960-mRNA-1:exon:6768;Parent=Msa0059960-mRNA-1
chr1_2 exon 17507545 17507575 17507545 ID=Msa0059960-mRNA-1:exon:6769;Parent=Msa0059960-mRNA-1
chr1_2 five_prime_UTR 17503315 17503487 17503315 ID=Msa0059960-mRNA-1:five_prime_utr;Parent=Msa0059960-mRNA-1
chr1_2 five_prime_UTR 17504486 17504557 17504486 ID=Msa0059960-mRNA-1:five_prime_utr;Parent=Msa0059960-mRNA-1
chr1_2 five_prime_UTR 17504674 17504683 17504674 ID=Msa0059960-mRNA-1:five_prime_utr;Parent=Msa0059960-mRNA-1
chr1_2 CDS 17504684 17506419 17504684 ID=Msa0059960-mRNA-1:cds;Parent=Msa0059960-mRNA-1
chr1_2 CDS 17507545 17507575 17507545 ID=Msa0059960-mRNA-1:cds;Parent=Msa0059960-mRNA-1
Gene Sequence

>Msa0059960

ATGTCATTGATTATATCTCCGGAGTTGGCTGATGCGTCGTATGGGAATGCCAAGCTATACTCGTTAAAGGGAGCCGATGTTACACCTGGCTTGTCCTCCCATGATTTTGCTCCTGATAAGCATAGAAGCATGTACATGAATAATTCTTACTCGAGTGAGAGTTACGAAAAGTACTTCCTTGATTCACCTACAGAGGAACTGATAGAACCGTCTAGTTCTGGGATATCAGGAAATTCTGTTCATGCTGATGGTGATTCTTCGTACCAGCTCAGAGCTAATTCAGGAGGTTCTATGGTTACTGATAACCAGTTTGAAACTTATGTTTCGTCTACAAGGCAGCGTGATGCATATCAGTCCAATTTTGAATCAGATTTCTTGGAAAGTGAAAGCCCTGATTGCTTTGAATTTGACGGTGATATGCGATTGAAAATACAAGAATTGGAGAGGGCACTGCTTGGTGACAATAATGATGAGTGTGATGACGATGAGGAAAACATGTTTAGGACTGTTCAGAGCATGGAGATTGATCCTGACTTTACTGAGTGTGCCAATCCCATTCAGAACAAGTCGCATCATGACTCGCCAAAGGAATCTTTGTCCTCAGATTCAAATCTTAGTAGCACCAGTAGCACCAAAGAAATATCACAAAACTTTCCTCGGACCCCGAAGCAATTACTCTATGAATGTGCTAGCGCACTTTCAGAAGGAAATGAAGTAAAAGCCTCAGCTATGATCGATGATCTCAGGCAGTTGGTTTCAATTCAGGGAGAACCTTCGGATAGAATTGCAGCCTACATGGTGGAAGGACTTGCGGCTCGTTTGGCTTCATCGGGGAAATGTATTTATAAAGCTTTGAAATGCAAGGAACCTCCGTCTTCTGATCGTCTAGCAGCAATGCAGATACTATTCGAGGTGTGCCCATGTTTTAAGTTTGGATTTATTGCTGCTAATGGTGCTATCTCTGAGGCTATTAAAGATGATAAGAAGGTTCACATTATAGATTTTGATATCAACCAAGGAAGTCAATATATAACCCTGATACAAACACTTGCTTCTAGGCCAGGGAAGCCACCTTATGTGAGATTGACCGGTGTTGATGATCCCGAATCCGTGCAACGATCTGTTGGAGGCCTAAGTATCATAGGTCAAAGACTTGAAAAGCTAGCAGAAGTACTCGGATTGCCATTTGAATTTCGTGCCGTAGCATCAAGGTCTTCAATTGTTACTCCATCAATGCTCAACTGTCGTCCCGGGGAAGCTCTTGTGGTGAACTTTGCATTCCAGCTTCATCACATGCCCGATGAAAGTGTATCAACGGTAAACGAACGAGACCAGCTTCTCCGCATGGTTAAGAGCCTTAATCCAAAACTTGTGACAGTTGTGGAACAAGATGTGAACACCAACACTTCCCCCTTTCTCCCAAGATTTGTTGAAGCCTATAATTACTACTCTGCTGTGTATGAGTCACTCGACGCCACTCTTCCGAGAGAGAGCCAGGATAGGTTGAATGTTGAAAGGCAATGCTTGGCGAGGGACATTGTCAATGTCATTGCGTGTGAGGGCGAGGATAGAATAGAACGGTATGAAGTTGCCGGAAAATGGAGGGCAAGGATGAAAATGGCTGGATTCACTTCATCACCAATGAGCACAAATGTCAAAGAAGCAATCAGGGAACTCATAAGGCAGTACTGTGACAAGTACAAGATAATAGAGGAAATGGGGGGACTTCATTTTGGTAAGAACTTTGTTCAACAGCAAATGTTATGA
Protein sequence

>Msa0059960

MSLIISPELADASYGNAKLYSLKGADVTPGLSSHDFAPDKHRSMYMNNSYSSESYEKYFLDSPTEELIEPSSSGISGNSVHADGDSSYQLRANSGGSMVTDNQFETYVSSTRQRDAYQSNFESDFLESESPDCFEFDGDMRLKIQELERALLGDNNDECDDDEENMFRTVQSMEIDPDFTECANPIQNKSHHDSPKESLSSDSNLSSTSSTKEISQNFPRTPKQLLYECASALSEGNEVKASAMIDDLRQLVSIQGEPSDRIAAYMVEGLAARLASSGKCIYKALKCKEPPSSDRLAAMQILFEVCPCFKFGFIAANGAISEAIKDDKKVHIIDFDINQGSQYITLIQTLASRPGKPPYVRLTGVDDPESVQRSVGGLSIIGQRLEKLAEVLGLPFEFRAVASRSSIVTPSMLNCRPGEALVVNFAFQLHHMPDESVSTVNERDQLLRMVKSLNPKLVTVVEQDVNTNTSPFLPRFVEAYNYYSAVYESLDATLPRESQDRLNVERQCLARDIVNVIACEGEDRIERYEVAGKWRARMKMAGFTSSPMSTNVKEAIRELIRQYCDKYKIIEEMGGLHFGKNFVQQQML*