AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1004520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1004520 MtrunA17_Chr7g0214731 98.330 479 8 0 1 479 158 636 0.0 982
Msa1004520 MtrunA17_Chr4g0005901 42.500 440 239 7 21 455 141 571 1.33e-117 357
Msa1004520 MtrunA17_Chr7g0241741 42.424 462 244 10 1 455 115 561 5.63e-117 355
Msa1004520 MtrunA17_Chr8g0375311 41.950 441 242 7 21 455 150 582 7.10e-112 342
Msa1004520 MtrunA17_Chr3g0104011 41.364 440 247 6 20 455 131 563 4.30e-111 340
Msa1004520 MtrunA17_Chr8g0375291 40.839 453 255 6 1 449 133 576 2.37e-106 328
Msa1004520 MtrunA17_Chr3g0142101 38.681 455 262 5 21 473 145 584 3.86e-106 327
Msa1004520 MtrunA17_Chr7g0271221 39.437 426 243 5 33 457 137 548 4.49e-104 322
Msa1004520 MtrunA17_Chr3g0142091 38.938 452 259 5 21 470 138 574 8.27e-102 316
Msa1004520 MtrunA17_Chr1g0193391 38.407 427 248 5 33 458 137 549 3.47e-100 313
Msa1004520 MtrunA17_Chr3g0133341 39.456 441 249 8 18 456 127 551 1.31e-98 307
Msa1004520 MtrunA17_Chr8g0375321 38.950 457 266 6 1 453 130 577 5.16e-98 306
Msa1004520 MtrunA17_Chr8g0375341 38.813 438 260 4 21 455 150 582 1.46e-97 305
Msa1004520 MtrunA17_Chr7g0261661 37.527 461 271 8 20 474 26 475 1.49e-97 303
Msa1004520 MtrunA17_Chr8g0375351 39.130 437 256 5 21 453 150 580 3.87e-97 304
Msa1004520 MtrunA17_Chr8g0375331 39.387 457 264 6 1 453 117 564 7.76e-96 300
Msa1004520 MtrunA17_Chr2g0284701 35.776 464 279 9 6 457 134 590 3.75e-94 297
Msa1004520 MtrunA17_Chr7g0261811 36.364 462 284 5 20 480 140 592 1.10e-93 296
Msa1004520 MtrunA17_Chr7g0261881 36.463 458 277 7 20 476 140 584 1.19e-92 293
Msa1004520 MtrunA17_Chr8g0375361 38.497 439 260 6 21 455 149 581 3.48e-92 291
Msa1004520 MtrunA17_Chr1g0213711 37.300 437 255 6 23 453 132 555 1.05e-91 290
Msa1004520 MtrunA17_Chr4g0069581 36.865 453 267 10 33 480 106 544 1.07e-91 289
Msa1004520 MtrunA17_Chr5g0422881 37.284 464 277 9 21 477 131 587 1.20e-91 290
Msa1004520 MtrunA17_Chr7g0261771 35.714 462 286 7 20 480 144 595 1.57e-91 290
Msa1004520 MtrunA17_Chr7g0255621 35.256 468 290 7 20 481 130 590 2.30e-90 287
Msa1004520 MtrunA17_Chr7g0261791 36.463 458 277 7 1 454 122 569 6.33e-90 286
Msa1004520 MtrunA17_Chr7g0218851 38.128 438 253 9 21 453 158 582 1.25e-89 285
Msa1004520 MtrunA17_Chr1g0187241 35.118 467 283 8 10 473 130 579 1.48e-89 285
Msa1004520 MtrunA17_Chr7g0214461 36.782 435 261 6 21 453 190 612 1.66e-89 286
Msa1004520 MtrunA17_Chr3g0114501 37.162 444 265 9 19 457 129 563 1.04e-88 282
Msa1004520 MtrunA17_Chr5g0399061 34.437 453 275 7 20 461 137 578 2.02e-87 279
Msa1004520 MtrunA17_Chr5g0439381 35.762 453 260 6 11 458 133 559 1.20e-86 277
Msa1004520 MtrunA17_Chr4g0059981 36.638 464 266 9 21 470 144 593 2.92e-86 276
Msa1004520 MtrunA17_Chr7g0255601 35.520 442 273 6 20 455 137 572 9.00e-86 275
Msa1004520 MtrunA17_Chr8g0362711 35.435 460 267 8 10 455 128 571 1.81e-85 273
Msa1004520 MtrunA17_Chr8g0362801 35.841 452 275 7 11 455 18 461 2.99e-84 268
Msa1004520 MtrunA17_Chr5g0415791 36.424 453 273 7 10 455 133 577 5.41e-84 271
Msa1004520 MtrunA17_Chr1g0166711 35.153 458 287 5 1 457 118 566 6.04e-84 270
Msa1004520 MtrunA17_Chr3g0112611 34.874 476 288 11 1 469 125 585 6.39e-84 270
Msa1004520 MtrunA17_Chr7g0255611 32.762 467 295 7 20 473 139 599 2.02e-83 269
Msa1004520 MtrunA17_Chr7g0240381 33.769 459 278 9 1 455 122 558 1.71e-82 266
Msa1004520 MtrunA17_Chr3g0112671 32.533 458 285 8 1 456 126 561 1.93e-82 266
Msa1004520 MtrunA17_Chr1g0166731 34.279 458 291 5 1 457 15 463 3.66e-82 262
Msa1004520 MtrunA17_Chr7g0261851 37.500 440 257 7 20 454 141 567 4.60e-82 265
Msa1004520 MtrunA17_Chr4g0056431 36.166 459 279 8 18 469 135 586 1.46e-81 264
Msa1004520 MtrunA17_Chr8g0353031 36.161 448 265 7 30 464 120 559 1.69e-81 263
Msa1004520 MtrunA17_Chr8g0379971 36.717 463 264 9 21 457 134 593 1.80e-81 264
Msa1004520 MtrunA17_Chr7g0217461 36.526 449 276 5 21 464 136 580 2.47e-81 263
Msa1004520 MtrunA17_Chr4g0011471 34.361 454 274 8 9 453 119 557 3.48e-81 262
Msa1004520 MtrunA17_Chr5g0399071 34.783 460 280 6 20 470 136 584 5.50e-81 262
Msa1004520 MtrunA17_Chr3g0082631 34.169 439 268 8 33 458 32 462 8.63e-81 258
Msa1004520 MtrunA17_Chr4g0059971 35.088 456 260 11 20 455 145 584 1.08e-80 261
Msa1004520 MtrunA17_Chr5g0400371 34.240 441 276 6 20 453 223 656 1.55e-80 263
Msa1004520 MtrunA17_Chr2g0276751 34.459 444 264 7 15 455 144 563 9.07e-80 259
Msa1004520 MtrunA17_Chr4g0063591 36.547 446 255 9 20 455 132 559 1.01e-79 258
Msa1004520 MtrunA17_Chr1g0166741 33.487 433 270 6 1 431 120 536 9.90e-79 256
Msa1004520 MtrunA17_Chr5g0415961 35.333 450 264 9 21 455 135 572 1.27e-78 256
Msa1004520 MtrunA17_Chr8g0362781 32.000 450 286 7 18 456 142 582 3.15e-78 255
Msa1004520 MtrunA17_Chr1g0166771 33.864 440 273 6 1 438 84 507 3.41e-78 254
Msa1004520 MtrunA17_Chr8g0390331 37.079 445 252 8 20 452 125 553 5.11e-78 254
Msa1004520 MtrunA17_Chr4g0008651 32.680 459 289 9 1 455 127 569 1.71e-77 253
Msa1004520 MtrunA17_Chr4g0059991 32.636 478 292 10 21 480 134 599 2.86e-77 253
Msa1004520 MtrunA17_Chr1g0166791 32.096 458 302 5 1 457 117 566 3.14e-77 253
Msa1004520 MtrunA17_Chr8g0379991 35.908 479 259 11 21 457 94 566 7.24e-77 251
Msa1004520 MtrunA17_Chr4g0054891 33.409 443 272 6 20 453 221 649 1.89e-76 252
Msa1004520 MtrunA17_Chr4g0014781 32.883 444 269 9 18 454 129 550 3.94e-76 249
Msa1004520 MtrunA17_Chr2g0331481 34.402 468 280 10 21 477 137 588 2.60e-75 248
Msa1004520 MtrunA17_Chr2g0331471 34.047 467 283 9 21 477 200 651 5.65e-75 249
Msa1004520 MtrunA17_Chr4g0014861 32.184 435 277 7 28 459 13 432 1.41e-74 241
Msa1004520 MtrunA17_Chr1g0157601 34.423 459 281 8 1 452 108 553 4.07e-74 244
Msa1004520 MtrunA17_Chr4g0014801 31.973 441 271 9 21 454 132 550 5.92e-74 243
Msa1004520 MtrunA17_Chr4g0014841 31.494 435 277 7 28 459 137 553 4.50e-73 241
Msa1004520 MtrunA17_Chr4g0014791 32.802 439 266 8 28 459 137 553 2.10e-72 239
Msa1004520 MtrunA17_Chr4g0003761 33.191 467 288 9 21 475 142 596 7.87e-72 238
Msa1004520 MtrunA17_Chr2g0331521 33.784 444 269 10 20 452 134 563 1.57e-71 237
Msa1004520 MtrunA17_Chr4g0014871 32.432 444 276 9 21 459 133 557 1.67e-70 234
Msa1004520 MtrunA17_Chr5g0415841 29.978 457 299 8 18 466 25 468 1.43e-69 229
Msa1004520 MtrunA17_Chr3g0077151 32.110 436 264 8 28 455 132 543 3.84e-69 230
Msa1004520 MtrunA17_Chr1g0157621 32.751 458 289 8 1 452 73 517 4.39e-68 228
Msa1004520 MtrunA17_Chr1g0157611 33.479 457 280 9 5 452 113 554 6.48e-68 227
Msa1004520 MtrunA17_Chr4g0060001 31.718 454 286 10 20 463 141 580 6.10e-67 226
Msa1004520 MtrunA17_Chr2g0331461 33.913 460 273 10 21 464 137 581 9.47e-67 226
Msa1004520 MtrunA17_Chr1g0147631 34.924 461 273 9 21 469 136 581 4.67e-66 223
Msa1004520 MtrunA17_Chr2g0331511 32.456 456 282 10 21 464 133 574 4.62e-65 221
Msa1004520 MtrunA17_Chr1g0177611 31.567 434 269 8 27 452 22 435 4.65e-65 216
Msa1004520 MtrunA17_Chr4g0011041 28.879 464 300 9 3 453 133 579 2.31e-64 218
Msa1004520 MtrunA17_Chr1g0177591 31.448 442 274 9 19 452 144 564 4.52e-64 217
Msa1004520 MtrunA17_Chr1g0177631 31.797 434 268 8 27 452 22 435 3.89e-63 211
Msa1004520 MtrunA17_Chr3g0142121 38.542 288 160 5 185 470 11 283 4.71e-62 204
Msa1004520 MtrunA17_Chr7g0261751 34.097 349 188 4 20 331 144 487 1.80e-59 204
Msa1004520 MtrunA17_Chr7g0240211 28.153 444 302 7 18 452 21 456 1.16e-57 198
Msa1004520 MtrunA17_Chr7g0240181 28.153 444 302 7 18 452 21 456 1.52e-57 197
Msa1004520 MtrunA17_Chr7g0235521 28.153 444 302 7 18 452 21 456 1.23e-56 195
Msa1004520 MtrunA17_Chr7g0240131 28.153 444 302 7 18 452 21 456 2.86e-56 194
Msa1004520 MtrunA17_Chr7g0234151 30.930 430 253 10 28 452 28 418 1.10e-54 188
Msa1004520 MtrunA17_Chr7g0234381 31.628 430 250 10 28 452 28 418 1.40e-54 188
Msa1004520 MtrunA17_Chr7g0240271 28.315 445 300 8 18 452 132 567 8.75e-54 190
Msa1004520 MtrunA17_Chr7g0240151 27.928 444 285 8 18 452 21 438 1.26e-53 187
Msa1004520 MtrunA17_Chr7g0240281 29.888 445 291 8 18 450 132 567 3.85e-53 188
Msa1004520 MtrunA17_Chr7g0234181 30.930 430 253 10 28 452 23 413 4.02e-53 184
Msa1004520 MtrunA17_Chr3g0112631 34.657 277 171 6 184 456 3 273 3.21e-52 178
Msa1004520 MtrunA17_Chr4g0060071 34.000 300 185 6 159 456 11 299 1.83e-51 176
Msa1004520 MtrunA17_Chr7g0261731 40.476 210 120 2 20 229 144 348 1.67e-45 162
Msa1004520 MtrunA17_Chr2g0325961 28.884 457 265 13 20 455 131 548 6.03e-45 165
Msa1004520 MtrunA17_Chr2g0326211 26.316 456 277 12 20 455 131 547 3.77e-44 163
Msa1004520 MtrunA17_Chr2g0325951 28.281 442 286 8 20 455 131 547 6.22e-44 162
Msa1004520 MtrunA17_Chr6g0451841 26.573 429 285 9 42 457 127 538 1.14e-41 155
Msa1004520 MtrunA17_Chr3g0142111 40.351 171 102 0 21 191 156 326 1.67e-36 137
Msa1004520 MtrunA17_Chr7g0261721 32.394 213 140 3 256 468 6 214 2.35e-32 123
Msa1004520 MtrunA17_Chr1g0147591 35.000 200 111 5 34 223 5 195 6.11e-30 115
Msa1004520 MtrunA17_Chr3g0112651 32.124 193 123 4 1 193 27 211 2.70e-27 108
Msa1004520 MtrunA17_Chr7g0261831 35.714 154 92 4 138 289 1 149 2.12e-18 84.0
Msa1004520 MtrunA17_Chr4g0060061 32.231 121 82 0 21 141 8 128 9.70e-17 76.6
Msa1004520 MtrunA17_Chr2g0326221 25.688 218 128 5 239 455 39 223 1.76e-14 72.8
Msa1004520 MtrunA17_Chr1g0147581 36.066 122 69 2 308 429 4 116 7.02e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1004520 AT5G13400.1 78.242 455 97 1 1 455 159 611 0.0 760
Msa1004520 AT2G02040.1 43.151 438 241 5 21 455 149 581 1.79e-116 354
Msa1004520 AT1G62200.1 43.925 428 224 5 21 445 163 577 4.72e-112 343
Msa1004520 AT3G54140.1 40.319 439 251 6 21 455 132 563 1.69e-111 341
Msa1004520 AT3G54140.2 40.319 439 251 6 21 455 132 563 1.69e-111 341
Msa1004520 AT5G01180.2 41.435 432 243 5 28 455 138 563 4.54e-111 340
Msa1004520 AT5G01180.3 41.435 432 243 5 28 455 138 563 4.54e-111 340
Msa1004520 AT5G01180.1 41.435 432 243 5 28 455 138 563 4.54e-111 340
Msa1004520 AT1G62200.2 41.357 457 223 6 21 445 163 606 4.70e-106 328
Msa1004520 AT1G62200.3 41.357 457 223 6 21 445 177 620 5.21e-106 329
Msa1004520 AT2G02020.1 38.991 436 221 6 21 454 150 542 1.76e-93 294
Msa1004520 AT3G21670.1 35.604 455 259 5 11 457 132 560 1.28e-88 283
Msa1004520 AT2G26690.3 37.061 456 259 11 21 466 88 525 2.37e-87 278
Msa1004520 AT2G26690.2 37.061 456 259 11 21 466 100 537 2.51e-87 278
Msa1004520 AT3G54450.1 34.247 438 278 5 24 455 109 542 3.16e-87 278
Msa1004520 AT2G26690.1 37.061 456 259 11 21 466 133 570 5.22e-87 278
Msa1004520 AT1G69850.1 35.808 458 264 7 20 456 132 580 7.16e-87 278
Msa1004520 AT5G46050.2 34.722 432 272 6 1 431 6 428 1.02e-86 274
Msa1004520 AT5G46050.1 34.722 432 272 6 1 431 118 540 2.57e-85 274
Msa1004520 AT2G37900.2 33.913 460 279 9 1 456 50 488 2.60e-85 271
Msa1004520 AT2G40460.1 36.054 441 268 7 19 457 129 557 4.02e-85 273
Msa1004520 AT2G37900.1 33.913 460 279 9 1 456 129 567 1.63e-84 271
Msa1004520 AT5G46040.1 35.011 457 284 7 20 473 137 583 3.77e-84 271
Msa1004520 AT1G68570.2 36.689 447 273 6 21 461 102 544 8.99e-84 269
Msa1004520 AT1G68570.1 36.689 447 273 6 21 461 132 574 2.09e-83 269
Msa1004520 AT1G32450.1 34.755 469 270 10 21 469 147 599 2.48e-83 269
Msa1004520 AT1G69870.1 33.978 465 291 8 20 478 161 615 6.29e-83 268
Msa1004520 AT3G53960.1 33.906 466 279 12 1 456 128 574 8.00e-82 265
Msa1004520 AT1G27040.3 34.096 437 273 5 20 454 105 528 8.78e-81 261
Msa1004520 AT1G27040.2 34.096 437 273 5 20 454 133 556 1.66e-80 261
Msa1004520 AT1G27040.1 34.096 437 273 5 20 454 137 560 1.73e-80 261
Msa1004520 AT1G33440.1 34.409 465 281 10 20 477 149 596 4.92e-80 260
Msa1004520 AT1G59740.1 34.793 434 270 5 16 447 147 569 1.16e-78 256
Msa1004520 AT1G12110.1 34.573 457 281 8 20 469 137 582 1.54e-78 256
Msa1004520 AT5G19640.1 35.747 442 247 10 21 453 169 582 2.27e-78 256
Msa1004520 AT4G21680.1 35.586 444 267 7 21 454 138 572 3.39e-78 255
Msa1004520 AT3G47960.1 35.045 448 271 8 20 455 169 608 2.40e-77 254
Msa1004520 AT5G62730.2 33.708 445 273 9 25 458 96 529 8.67e-77 250
Msa1004520 AT5G62730.1 33.708 445 273 9 25 458 155 588 2.87e-76 250
Msa1004520 AT3G16180.1 35.456 471 270 11 20 468 133 591 3.45e-76 250
Msa1004520 AT5G62680.1 33.857 446 277 7 21 455 154 592 6.52e-76 250
Msa1004520 AT1G52190.1 35.347 447 263 9 20 452 132 566 3.44e-75 248
Msa1004520 AT2G02020.2 35.550 436 215 7 21 454 150 521 3.68e-75 246
Msa1004520 AT1G18880.1 31.839 446 284 6 21 455 130 566 1.44e-74 246
Msa1004520 AT1G27080.2 32.506 443 274 8 20 455 82 506 1.91e-72 239
Msa1004520 AT1G27080.1 32.506 443 274 8 20 455 124 548 4.62e-72 239
Msa1004520 AT3G25260.1 31.050 438 253 10 19 452 123 515 1.19e-64 218
Msa1004520 AT1G72140.1 31.279 438 261 7 28 456 147 553 3.77e-64 217
Msa1004520 AT1G72120.1 33.643 431 250 11 36 455 147 552 7.58e-64 216
Msa1004520 AT1G22550.1 32.346 439 269 10 24 455 142 559 2.35e-63 215
Msa1004520 AT1G72125.1 31.090 431 273 8 36 459 147 560 7.85e-62 211
Msa1004520 AT1G22570.2 29.888 445 284 9 20 455 98 523 4.80e-61 208
Msa1004520 AT1G22570.1 29.888 445 284 9 20 455 135 560 1.10e-60 208
Msa1004520 AT5G28470.2 30.023 443 286 10 20 452 125 553 6.33e-60 206
Msa1004520 AT1G69860.1 32.043 465 276 10 4 455 112 549 9.36e-60 206
Msa1004520 AT3G45650.1 30.667 450 276 11 11 455 125 543 1.91e-59 205
Msa1004520 AT1G22540.1 30.989 455 285 12 1 452 121 549 3.63e-59 204
Msa1004520 AT1G72130.2 29.120 443 268 10 28 460 14 420 9.94e-59 199
Msa1004520 AT1G72130.3 29.120 443 268 10 28 460 14 420 9.94e-59 199
Msa1004520 AT3G45660.1 30.000 470 286 13 9 472 122 554 1.94e-58 202
Msa1004520 AT3G45660.3 30.000 470 286 13 9 472 122 554 1.94e-58 202
Msa1004520 AT5G28470.1 30.023 443 284 11 20 452 125 551 2.43e-58 202
Msa1004520 AT3G45660.2 30.000 470 286 13 9 472 143 575 2.85e-58 202
Msa1004520 AT1G72130.1 28.358 469 279 11 2 460 117 538 2.02e-57 199
Msa1004520 AT3G25280.1 29.907 428 260 11 28 452 131 521 9.75e-56 194
Msa1004520 AT3G45650.2 28.992 476 276 12 11 455 125 569 7.81e-54 190
Msa1004520 AT3G45710.1 28.541 466 293 12 11 466 124 559 6.78e-53 187
Msa1004520 AT3G45680.1 29.310 464 291 12 11 468 123 555 1.04e-51 184
Msa1004520 AT3G45720.1 30.088 452 276 12 11 455 122 540 1.73e-51 183
Msa1004520 AT3G45700.1 29.464 448 279 10 11 455 119 532 2.21e-51 183
Msa1004520 AT5G14940.1 25.420 476 285 11 19 458 103 544 1.46e-46 170
Msa1004520 AT3G01350.1 26.205 477 302 10 19 464 103 560 2.54e-44 164
Msa1004520 AT3G45690.1 27.130 446 257 12 11 455 121 499 6.68e-42 156
Msa1004520 AT5G11570.1 25.390 449 250 11 8 452 109 476 2.42e-33 132
Msa1004520 AT2G38100.2 26.126 444 274 15 28 452 76 484 5.22e-24 105
Msa1004520 AT2G38100.1 26.126 444 274 15 28 452 92 500 8.97e-24 104
Msa1004520 AT2G38100.3 26.126 444 274 15 28 452 98 506 1.22e-23 104

Find 115 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACCTAAGTGAGTGTTTA+AGG 0.112269 7_1:+2024682 None:intergenic
GAACATTAAACTTGATAATT+TGG 0.135880 7_1:-2024033 Msa1004520:CDS
TGCCAAATTTAAGTGCTTAT+TGG 0.163181 7_1:-2024406 Msa1004520:CDS
GGTCTATGTTCAAATGAAAT+TGG 0.164056 7_1:-2025507 Msa1004520:CDS
ATTGCTGCCTTCTTTATATT+TGG 0.176863 7_1:-2027338 Msa1004520:intron
GCAGAAAGAATGGCTTATTT+TGG 0.182047 7_1:-2027028 Msa1004520:five_prime_UTR
TTGATTCAATATTGCTTAAT+TGG 0.195137 7_1:-2024380 Msa1004520:CDS
TTGGGTTGGGGTTCTGCTTT+TGG 0.200637 7_1:-2025488 Msa1004520:CDS
AACCAATAAGCACTTAAATT+TGG 0.204333 7_1:+2024404 None:intergenic
TTCTAGCTAGTTTAAATATA+TGG 0.208193 7_1:-2023981 Msa1004520:exon
TTGCTCATACTGATGATTTC+AGG 0.218331 7_1:-2025250 Msa1004520:intron
AATGCAGTCAATAATTTCTT+AGG 0.237323 7_1:-2026941 Msa1004520:five_prime_UTR
AGACCATGTGTATCTTCTTT+TGG 0.243431 7_1:-2025641 Msa1004520:CDS
GTCCCTTGTTGAGGTGCTTC+TGG 0.246454 7_1:+2027515 None:intergenic
TCACAAGCTTCTTCTGTTCT+TGG 0.246695 7_1:-2026914 Msa1004520:five_prime_UTR
CAAAGAATTGGAATTGGTTT+AGG 0.253602 7_1:-2024527 Msa1004520:CDS
TGTTGGGTTGTACGAGGTTC+CGG 0.269004 7_1:-2025306 Msa1004520:CDS
AAATATACCCCTCACTTTAG+AGG 0.270126 7_1:+2023936 None:intergenic
AGGTTCCATTGACTTGGATT+AGG 0.295324 7_1:+2024830 None:intergenic
ATGCATACCTTGGACGTTAC+TGG 0.310134 7_1:-2026877 Msa1004520:five_prime_UTR
TCTACACCTGTTAATATTCA+TGG 0.321471 7_1:-2027398 Msa1004520:five_prime_UTR
GAGGATAGAAGGATGGCTTT+AGG 0.330296 7_1:-2027440 Msa1004520:five_prime_UTR
GACCAATTATCACTTCTATT+AGG 0.331157 7_1:-2026018 Msa1004520:CDS
TTCTATCTTTCTGTGACAAT+TGG 0.332881 7_1:-2025554 Msa1004520:CDS
TTCCTAATAGAAGTGATAAT+TGG 0.337644 7_1:+2026016 None:intergenic
TGAAAAGTGGTACAAAAGTT+TGG 0.338858 7_1:+2024588 None:intergenic
GGGTAGAAGATCTGCTATAA+AGG 0.340306 7_1:-2025285 Msa1004520:CDS
AAAGTGAGGGGTATATTTAA+AGG 0.343643 7_1:-2023931 Msa1004520:three_prime_UTR
GCTGCATTAGCTGGTGGATT+AGG 0.344505 7_1:-2024269 Msa1004520:CDS
GCATCTCAGCTTCAAAGAAT+TGG 0.351587 7_1:-2024539 Msa1004520:CDS
TGTTCAAATGAAATTGGGTT+GGG 0.357013 7_1:-2025501 Msa1004520:CDS
GGTTCCATTGACTTGGATTA+GGG 0.361442 7_1:+2024831 None:intergenic
CAGCTTCAAAGAATTGGAAT+TGG 0.361704 7_1:-2024533 Msa1004520:CDS
GTGTGTTAGAGTTAGTAAAA+TGG 0.369659 7_1:-2027558 Msa1004520:five_prime_UTR
CAAGCTTCTTCTGTTCTTGG+TGG 0.377343 7_1:-2026911 Msa1004520:five_prime_UTR
AAAACCGGCATGCATGTAAC+TGG 0.377966 7_1:+2024650 None:intergenic
AGGATAGAAGGATGGCTTTA+GGG 0.379409 7_1:-2027439 Msa1004520:five_prime_UTR
CAGATAAAAGACAAGAATCT+TGG 0.379936 7_1:-2024193 Msa1004520:CDS
GTTGGGTTGTACGAGGTTCC+GGG 0.395345 7_1:-2025305 Msa1004520:CDS
TCATTGAGTCTGAGGATAGA+AGG 0.397462 7_1:-2027451 Msa1004520:five_prime_UTR
GTTCTATACATAACAGCATT+TGG 0.401544 7_1:-2025949 Msa1004520:CDS
TCTGCTTATGCTGCATTAGC+TGG 0.402823 7_1:-2024278 Msa1004520:CDS
CGAAAGAGAAAAGAATCATT+TGG 0.406039 7_1:-2025341 Msa1004520:CDS
TAAGAAATTATTGACTGCAT+TGG 0.408173 7_1:+2026943 None:intergenic
GAGATCATTAAAATCTTAAT+CGG 0.412263 7_1:+2023873 None:intergenic
ATGATTGTGCAAGCTGGAAT+TGG 0.416463 7_1:+2024758 None:intergenic
TGTTTAAGGTATATGCTTGT+TGG 0.437145 7_1:+2024696 None:intergenic
TTGATAATTTGGCAAACAAT+GGG 0.441596 7_1:-2024022 Msa1004520:CDS
TTGGTAATAGTGAGGTTGTT+GGG 0.448667 7_1:-2025322 Msa1004520:CDS
GACTGAGAGAGTTAGAAATT+CGG 0.450049 7_1:+2024718 None:intergenic
ACTTGAGCGACTCGTGTAAG+AGG 0.451347 7_1:+2025380 None:intergenic
TACACGAGTCGCTCAAGTAT+TGG 0.457764 7_1:-2025375 Msa1004520:CDS
ATGCATCACATAAAACATAA+AGG 0.459047 7_1:+2026985 None:intergenic
GTCTATGTTCAAATGAAATT+GGG 0.468317 7_1:-2025506 Msa1004520:CDS
AAATGAAATGGCAGAAAGAA+TGG 0.471770 7_1:-2027038 Msa1004520:five_prime_UTR
TAAGCACTTAAATTTGGCAT+TGG 0.472247 7_1:+2024410 None:intergenic
CTTGATAATTTGGCAAACAA+TGG 0.474016 7_1:-2024023 Msa1004520:CDS
TTTGGTAATAGTGAGGTTGT+TGG 0.478953 7_1:-2025323 Msa1004520:CDS
GTACAAAGGTTCCATTGACT+TGG 0.480579 7_1:+2024824 None:intergenic
GGTAGAAGATCTGCTATAAA+GGG 0.482404 7_1:-2025284 Msa1004520:CDS
GGTTCATTGGCAATAGCAAT+GGG 0.486016 7_1:-2025467 Msa1004520:CDS
TGGTTCATTGGCAATAGCAA+TGG 0.496742 7_1:-2025468 Msa1004520:CDS
TTGTATAGACATAGGTTGCC+AGG 0.499116 7_1:-2025410 Msa1004520:CDS
GTACACCTTTGTATAGACAT+AGG 0.505286 7_1:-2025418 Msa1004520:CDS
AGAAGGATGGCTTTAGGGCG+TGG 0.510086 7_1:-2027434 Msa1004520:five_prime_UTR
GCTTATGCTGCATTAGCTGG+TGG 0.511561 7_1:-2024275 Msa1004520:CDS
ATGAGGAATACACTTAGGCC+TGG 0.513514 7_1:+2024626 None:intergenic
TTTCTGCTCATTGAAGTGCA+TGG 0.520712 7_1:+2027491 None:intergenic
TAGAGGTATGTCATTTGCCA+TGG 0.522678 7_1:+2025976 None:intergenic
GCATTGGATGAACTTGCAAA+AGG 0.523734 7_1:+2026959 None:intergenic
GTTCAAATGAAATTGGGTTG+GGG 0.526250 7_1:-2025500 Msa1004520:CDS
TTAAGCATGATTGTGCAAGC+TGG 0.527229 7_1:+2024752 None:intergenic
ATGTTCAAATGAAATTGGGT+TGG 0.527545 7_1:-2025502 Msa1004520:CDS
TTTCTAGCTGATGCATACCT+TGG 0.527808 7_1:-2026887 Msa1004520:five_prime_UTR
ATAACAGCATTTGGAGCTGC+AGG 0.530231 7_1:-2025940 Msa1004520:intron
ATGGCCCTAATCCAAGTCAA+TGG 0.532912 7_1:-2024835 Msa1004520:CDS
CTACTTGTGTTACTGTACAA+AGG 0.543543 7_1:+2024810 None:intergenic
ATACATACCAAATATAAAGA+AGG 0.545633 7_1:+2027331 None:intergenic
CAGCACATAGATACAAATAC+AGG 0.546985 7_1:-2024079 Msa1004520:CDS
TATACCTTAAACACTCACTT+AGG 0.555464 7_1:-2024686 Msa1004520:CDS
AGTGTTGTTAAATCTGCCAC+TGG 0.556705 7_1:-2024221 Msa1004520:CDS
AGAATCATTTGGTAATAGTG+AGG 0.559681 7_1:-2025330 Msa1004520:CDS
GACACAACATGTTTATGAGA+TGG 0.562119 7_1:-2024057 Msa1004520:CDS
CCTGATGCTATGAAAAGCAT+TGG 0.563487 7_1:-2024302 Msa1004520:CDS
GCTCTACAATTGAAACAAGA+TGG 0.565645 7_1:-2024854 Msa1004520:CDS
CTAATATGAGGAATACACTT+AGG 0.566459 7_1:+2024621 None:intergenic
GGTTACTGCAGGAAATGAAA+TGG 0.566480 7_1:-2027050 Msa1004520:intron
GTATTAGTCCTCTAAAGTGA+GGG 0.567731 7_1:-2023944 Msa1004520:three_prime_UTR
GGTAATAGAGAGCTAATATG+AGG 0.570895 7_1:+2024609 None:intergenic
TCAATGAGCAGAAAGAAACT+TGG 0.574485 7_1:-2027482 Msa1004520:five_prime_UTR
CCAGTGATGCGTTTGAAAAG+TGG 0.576819 7_1:+2024575 None:intergenic
ACTTCCAGTTACATGCATGC+CGG 0.579964 7_1:-2024654 Msa1004520:CDS
TTAGGGCGTGGTTATACAAG+AGG 0.582655 7_1:-2027422 Msa1004520:five_prime_UTR
TATTCATGGAAAATCCATTG+AGG 0.592324 7_1:-2027384 Msa1004520:five_prime_UTR
GTGCATGGTGTCCCTTGTTG+AGG 0.594959 7_1:+2027506 None:intergenic
CGCATCACTGGTCATCCGCA+TGG 0.596174 7_1:-2024563 Msa1004520:CDS
TGTATTAGTCCTCTAAAGTG+AGG 0.600612 7_1:-2023945 Msa1004520:three_prime_UTR
CTTCACTACAATTTATCTCG+CGG 0.610444 7_1:-2026844 Msa1004520:intron
CGTGTAAGAGGACTTCCACC+TGG 0.612686 7_1:+2025392 None:intergenic
TGAGGTTGTTGGGTTGTACG+AGG 0.623104 7_1:-2025312 Msa1004520:CDS
TCACCAGAAGCACCTCAACA+AGG 0.630442 7_1:-2027518 Msa1004520:five_prime_UTR
TGAGTCTGAGGATAGAAGGA+TGG 0.636242 7_1:-2027447 Msa1004520:five_prime_UTR
TTTATAGCAGATCTTCTACC+CGG 0.639927 7_1:+2025287 None:intergenic
GTATTGTCCAGTAACGTCCA+AGG 0.640840 7_1:+2026870 None:intergenic
CACCAGAAGCACCTCAACAA+GGG 0.650042 7_1:-2027517 Msa1004520:five_prime_UTR
CTCTCATTAGATGCATGAGT+AGG 0.653011 7_1:-2027645 Msa1004520:five_prime_UTR
ACTGTGAGCCTGCAAAACCA+TGG 0.656363 7_1:-2025993 Msa1004520:CDS
TTGTCTCAAAACATCAACAC+TGG 0.656717 7_1:-2024170 Msa1004520:CDS
AATTGTAGCATTCACAGCAG+TGG 0.669454 7_1:-2025528 Msa1004520:CDS
TATTAGTCCTCTAAAGTGAG+GGG 0.677539 7_1:-2023943 Msa1004520:three_prime_UTR
GGCAACCTATGTCTATACAA+AGG 0.679165 7_1:+2025413 None:intergenic
TTATGTATAGAACAGTGTAG+AGG 0.694790 7_1:+2025959 None:intergenic
TATAGACATAGGTTGCCAGG+TGG 0.698387 7_1:-2025407 Msa1004520:CDS
GCTCCAAAAGAAGATACACA+TGG 0.731891 7_1:+2025638 None:intergenic
TGAAGCTGAGATGCACCATG+CGG 0.749014 7_1:+2024548 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTCAAGTTATTAA+TGG - chr7_1:2025725-2025744 Msa1004520:intron 10.0%
!!! TTTTAATTCTATATATGTTT+AGG - chr7_1:2026646-2026665 Msa1004520:intron 10.0%
!! AGAATGCATTAAAATATATT+AGG - chr7_1:2024365-2024384 Msa1004520:CDS 15.0%
!! ACTTATTATGATTATGAAAA+TGG - chr7_1:2024417-2024436 Msa1004520:CDS 15.0%
!!! ATTTTTTTTTGTTGCTTTTA+TGG - chr7_1:2024464-2024483 Msa1004520:CDS 15.0%
!!! AATGATATTTTACAATTTTG+TGG + chr7_1:2024863-2024882 None:intergenic 15.0%
!!! AAACTCACTTTTACATATAA+GGG + chr7_1:2023939-2023958 None:intergenic 20.0%
!!! AAAACTCACTTTTACATATA+AGG + chr7_1:2023940-2023959 None:intergenic 20.0%
!! ATACATACCAAATATAAAGA+AGG + chr7_1:2024210-2024229 None:intergenic 20.0%
!! TTAAGAGTGAATAAATAGAT+TGG - chr7_1:2024778-2024797 Msa1004520:CDS 20.0%
!!! CTAATTTTCCTATGAAATTT+AGG + chr7_1:2025095-2025114 None:intergenic 20.0%
!!! TTCATAGGAAAATTAGTTTA+TGG - chr7_1:2025099-2025118 Msa1004520:intron 20.0%
!! TGACATGTTAACTAAATATT+CGG + chr7_1:2025166-2025185 None:intergenic 20.0%
!!! AGTCTCTAATTTTTTTGAAT+TGG + chr7_1:2025247-2025266 None:intergenic 20.0%
!!! AGGTATTTTTTAGATATAGT+TGG - chr7_1:2025618-2025637 Msa1004520:CDS 20.0%
!! GTGTAAATAAATCATGTTTA+TGG + chr7_1:2026562-2026581 None:intergenic 20.0%
!! AAGAATAATATCTCTTACAA+TGG + chr7_1:2026604-2026623 None:intergenic 20.0%
!!! GAATTGGAATTAGTTTTATA+AGG + chr7_1:2026767-2026786 None:intergenic 20.0%
!! TTGATTCAATATTGCTTAAT+TGG - chr7_1:2027158-2027177 Msa1004520:intron 20.0%
!!! GAACATTAAACTTGATAATT+TGG - chr7_1:2027505-2027524 Msa1004520:five_prime_UTR 20.0%
!!! TTCTAGCTAGTTTAAATATA+TGG - chr7_1:2027557-2027576 Msa1004520:five_prime_UTR 20.0%
!! GATTTTCCATGAATATTAAC+AGG + chr7_1:2024149-2024168 None:intergenic 25.0%
!!! TAGTATATGTCATGTTTTTG+AGG - chr7_1:2024288-2024307 Msa1004520:CDS 25.0%
!!! ATGTTTTTGAGGTAAAAGAT+AGG - chr7_1:2024299-2024318 Msa1004520:CDS 25.0%
!!! TTTTTTTGTTGCTTTTATGG+TGG - chr7_1:2024467-2024486 Msa1004520:CDS 25.0%
! ATGCATCACATAAAACATAA+AGG + chr7_1:2024556-2024575 None:intergenic 25.0%
!! TAAGAAATTATTGACTGCAT+TGG + chr7_1:2024598-2024617 None:intergenic 25.0%
! AATGCAGTCAATAATTTCTT+AGG - chr7_1:2024597-2024616 Msa1004520:CDS 25.0%
! TTGCTAATTCACAAAAATGA+GGG + chr7_1:2024834-2024853 None:intergenic 25.0%
! TTTGCTAATTCACAAAAATG+AGG + chr7_1:2024835-2024854 None:intergenic 25.0%
! GTTAACTAGAAATGTTTCAT+TGG - chr7_1:2024937-2024956 Msa1004520:intron 25.0%
!!! TTGAGAACTTGATATTTTTG+TGG - chr7_1:2025362-2025381 Msa1004520:CDS 25.0%
!! ATTCCAAACTTTTATGAACA+GGG - chr7_1:2025436-2025455 Msa1004520:CDS 25.0%
! TTCCTAATAGAAGTGATAAT+TGG + chr7_1:2025525-2025544 None:intergenic 25.0%
!! GAAAAAGTTGATTACAAACT+AGG + chr7_1:2025800-2025819 None:intergenic 25.0%
! TTGATCAGAAAGATTAACTT+TGG + chr7_1:2025835-2025854 None:intergenic 25.0%
! GTCTATGTTCAAATGAAATT+GGG - chr7_1:2026032-2026051 Msa1004520:CDS 25.0%
!! TCTAACATGCTTTTCTTTAT+TGG - chr7_1:2026098-2026117 Msa1004520:intron 25.0%
!!! ATAGAAAGCATCAAAACTTT+TGG + chr7_1:2026346-2026365 None:intergenic 25.0%
! AACCAATAAGCACTTAAATT+TGG + chr7_1:2027137-2027156 None:intergenic 25.0%
!!! CAAGTTTGATTACTTTTACT+GGG - chr7_1:2027391-2027410 Msa1004520:five_prime_UTR 25.0%
!! TTGATAATTTGGCAAACAAT+GGG - chr7_1:2027516-2027535 Msa1004520:five_prime_UTR 25.0%
GTGTGTTAGAGTTAGTAAAA+TGG - chr7_1:2023980-2023999 Msa1004520:exon 30.0%
TCTACACCTGTTAATATTCA+TGG - chr7_1:2024140-2024159 Msa1004520:CDS 30.0%
TATTCATGGAAAATCCATTG+AGG - chr7_1:2024154-2024173 Msa1004520:CDS 30.0%
!! AGTTTTTGAAAGATCCTCAA+TGG + chr7_1:2024171-2024190 None:intergenic 30.0%
ATTGAGGATCTTTCAAAAAC+TGG - chr7_1:2024170-2024189 Msa1004520:CDS 30.0%
ATTGCTGCCTTCTTTATATT+TGG - chr7_1:2024200-2024219 Msa1004520:CDS 30.0%
CTTACGATATATGTGTATGT+TGG - chr7_1:2024243-2024262 Msa1004520:CDS 30.0%
TAGGATAAGATCTGAATTCT+TGG - chr7_1:2024318-2024337 Msa1004520:CDS 30.0%
AAATGAAATGGCAGAAAGAA+TGG - chr7_1:2024500-2024519 Msa1004520:CDS 30.0%
! TTTTGGACTTTCTGTTAACA+TGG - chr7_1:2024527-2024546 Msa1004520:CDS 30.0%
TGCTAATTCACAAAAATGAG+GGG + chr7_1:2024833-2024852 None:intergenic 30.0%
TTCATAGACAAATTAGTCCT+TGG + chr7_1:2024991-2025010 None:intergenic 30.0%
ATGAGTGACCTAAATTTCAT+AGG - chr7_1:2025084-2025103 Msa1004520:intron 30.0%
TTCTCAACAATCTGGATTAT+CGG + chr7_1:2025349-2025368 None:intergenic 30.0%
ATATCAAGTTCTCAACAATC+TGG + chr7_1:2025357-2025376 None:intergenic 30.0%
! CATTCCAAACTTTTATGAAC+AGG - chr7_1:2025435-2025454 Msa1004520:CDS 30.0%
! ATAATTGGTCACAGTTTTCT+TGG + chr7_1:2025510-2025529 None:intergenic 30.0%
GACCAATTATCACTTCTATT+AGG - chr7_1:2025520-2025539 Msa1004520:CDS 30.0%
TTATGTATAGAACAGTGTAG+AGG + chr7_1:2025582-2025601 None:intergenic 30.0%
GTTCTATACATAACAGCATT+TGG - chr7_1:2025589-2025608 Msa1004520:CDS 30.0%
CTGATCAACTTATTTACTCT+TGG - chr7_1:2025847-2025866 Msa1004520:intron 30.0%
! ATTCTACCATATTTCTGAAC+AGG - chr7_1:2025870-2025889 Msa1004520:intron 30.0%
CTTATACCTGTTCAGAAATA+TGG + chr7_1:2025879-2025898 None:intergenic 30.0%
TTCTATCTTTCTGTGACAAT+TGG - chr7_1:2025984-2026003 Msa1004520:CDS 30.0%
GGTCTATGTTCAAATGAAAT+TGG - chr7_1:2026031-2026050 Msa1004520:CDS 30.0%
ATGTTCAAATGAAATTGGGT+TGG - chr7_1:2026036-2026055 Msa1004520:CDS 30.0%
TGTTCAAATGAAATTGGGTT+GGG - chr7_1:2026037-2026056 Msa1004520:CDS 30.0%
! TTCTTTTCTCTTTCGATAAG+CGG + chr7_1:2026192-2026211 None:intergenic 30.0%
CGAAAGAGAAAAGAATCATT+TGG - chr7_1:2026197-2026216 Msa1004520:intron 30.0%
! AGAATCATTTGGTAATAGTG+AGG - chr7_1:2026208-2026227 Msa1004520:intron 30.0%
! TGTTTAAGGTATATGCTTGT+TGG + chr7_1:2026845-2026864 None:intergenic 30.0%
TATACCTTAAACACTCACTT+AGG - chr7_1:2026852-2026871 Msa1004520:five_prime_UTR 30.0%
TTTACCTAAGTGAGTGTTTA+AGG + chr7_1:2026859-2026878 None:intergenic 30.0%
CTAATATGAGGAATACACTT+AGG + chr7_1:2026920-2026939 None:intergenic 30.0%
TGAAAAGTGGTACAAAAGTT+TGG + chr7_1:2026953-2026972 None:intergenic 30.0%
! CAAAGAATTGGAATTGGTTT+AGG - chr7_1:2027011-2027030 Msa1004520:five_prime_UTR 30.0%
! TTTCGATTTTATCAGTTGCT+TGG - chr7_1:2027036-2027055 Msa1004520:five_prime_UTR 30.0%
! TTCGATTTTATCAGTTGCTT+GGG - chr7_1:2027037-2027056 Msa1004520:five_prime_UTR 30.0%
TAAGCACTTAAATTTGGCAT+TGG + chr7_1:2027131-2027150 None:intergenic 30.0%
TGCCAAATTTAAGTGCTTAT+TGG - chr7_1:2027132-2027151 Msa1004520:intron 30.0%
!!! TTTTTGCATTGTTGGTTTGT+TGG - chr7_1:2027193-2027212 Msa1004520:intron 30.0%
! TCTTTTATCTGAATTTCCAG+TGG + chr7_1:2027336-2027355 None:intergenic 30.0%
CAGATAAAAGACAAGAATCT+TGG - chr7_1:2027345-2027364 Msa1004520:five_prime_UTR 30.0%
!! GCAAGTTTGATTACTTTTAC+TGG - chr7_1:2027390-2027409 Msa1004520:five_prime_UTR 30.0%
! CTTGATAATTTGGCAAACAA+TGG - chr7_1:2027515-2027534 Msa1004520:five_prime_UTR 30.0%
! AAAGTGAGGGGTATATTTAA+AGG - chr7_1:2027607-2027626 Msa1004520:five_prime_UTR 30.0%
TCAATGAGCAGAAAGAAACT+TGG - chr7_1:2024056-2024075 Msa1004520:CDS 35.0%
! CATTTTCTTCATTGAGTCTG+AGG - chr7_1:2024079-2024098 Msa1004520:CDS 35.0%
ATCTTTCAAAAACTGGTGGT+TGG - chr7_1:2024177-2024196 Msa1004520:CDS 35.0%
! GCAGAAAGAATGGCTTATTT+TGG - chr7_1:2024510-2024529 Msa1004520:CDS 35.0%
CTTCACTACAATTTATCTCG+CGG - chr7_1:2024694-2024713 Msa1004520:CDS 35.0%
TTGGAGAGTTAACATTGATG+TGG + chr7_1:2024972-2024991 None:intergenic 35.0%
ACATCAATGTTAACTCTCCA+AGG - chr7_1:2024971-2024990 Msa1004520:intron 35.0%
TGAATAGGCAAATTGGTTGT+CGG + chr7_1:2025292-2025311 None:intergenic 35.0%
CTTTGAGTGAATAGGCAAAT+TGG + chr7_1:2025299-2025318 None:intergenic 35.0%
TGTTTCGACTTTGAGTGAAT+AGG + chr7_1:2025307-2025326 None:intergenic 35.0%
AAGCCCTGTTCATAAAAGTT+TGG + chr7_1:2025442-2025461 None:intergenic 35.0%
! TTTTATGAACAGGGCTTAAC+TGG - chr7_1:2025445-2025464 Msa1004520:CDS 35.0%
CCAACATGGATAAGATATGT+CGG + chr7_1:2025656-2025675 None:intergenic 35.0%
CAAACTAGGAAGAATTGACA+CGG + chr7_1:2025786-2025805 None:intergenic 35.0%
! AGACCATGTGTATCTTCTTT+TGG - chr7_1:2025897-2025916 Msa1004520:intron 35.0%
! TTTTGCTTCTTTCGTCGAAT+TGG + chr7_1:2025929-2025948 None:intergenic 35.0%
! GTTCAAATGAAATTGGGTTG+GGG - chr7_1:2026038-2026057 Msa1004520:CDS 35.0%
GTACACCTTTGTATAGACAT+AGG - chr7_1:2026120-2026139 Msa1004520:intron 35.0%
! TTTGGTAATAGTGAGGTTGT+TGG - chr7_1:2026215-2026234 Msa1004520:intron 35.0%
! TTGGTAATAGTGAGGTTGTT+GGG - chr7_1:2026216-2026235 Msa1004520:intron 35.0%
TTTATAGCAGATCTTCTACC+CGG + chr7_1:2026254-2026273 None:intergenic 35.0%
GGTAGAAGATCTGCTATAAA+GGG - chr7_1:2026254-2026273 Msa1004520:intron 35.0%
TTGCTCATACTGATGATTTC+AGG - chr7_1:2026288-2026307 Msa1004520:intron 35.0%
!! TTTCTGACACATGTCACTTT+CGG + chr7_1:2026539-2026558 None:intergenic 35.0%
GCTCTACAATTGAAACAAGA+TGG - chr7_1:2026684-2026703 Msa1004520:intron 35.0%
! CTACTTGTGTTACTGTACAA+AGG + chr7_1:2026731-2026750 None:intergenic 35.0%
GACTGAGAGAGTTAGAAATT+CGG + chr7_1:2026823-2026842 None:intergenic 35.0%
GGTAATAGAGAGCTAATATG+AGG + chr7_1:2026932-2026951 None:intergenic 35.0%
CAGCTTCAAAGAATTGGAAT+TGG - chr7_1:2027005-2027024 Msa1004520:five_prime_UTR 35.0%
!!! GCTGAAGTTTTTTGCATTGT+TGG - chr7_1:2027185-2027204 Msa1004520:intron 35.0%
TTGTCTCAAAACATCAACAC+TGG - chr7_1:2027368-2027387 Msa1004520:five_prime_UTR 35.0%
CAGCACATAGATACAAATAC+AGG - chr7_1:2027459-2027478 Msa1004520:five_prime_UTR 35.0%
GACACAACATGTTTATGAGA+TGG - chr7_1:2027481-2027500 Msa1004520:five_prime_UTR 35.0%
!! TGTATTAGTCCTCTAAAGTG+AGG - chr7_1:2027593-2027612 Msa1004520:five_prime_UTR 35.0%
!! GTATTAGTCCTCTAAAGTGA+GGG - chr7_1:2027594-2027613 Msa1004520:five_prime_UTR 35.0%
!! TATTAGTCCTCTAAAGTGAG+GGG - chr7_1:2027595-2027614 Msa1004520:five_prime_UTR 35.0%
AAATATACCCCTCACTTTAG+AGG + chr7_1:2027605-2027624 None:intergenic 35.0%
CTCTCATTAGATGCATGAGT+AGG - chr7_1:2023893-2023912 Msa1004520:three_prime_UTR 40.0%
TTTCTGCTCATTGAAGTGCA+TGG + chr7_1:2024050-2024069 None:intergenic 40.0%
TCATTGAGTCTGAGGATAGA+AGG - chr7_1:2024087-2024106 Msa1004520:CDS 40.0%
! AGGATAGAAGGATGGCTTTA+GGG - chr7_1:2024099-2024118 Msa1004520:CDS 40.0%
GAGGATCTTTCAAAAACTGG+TGG - chr7_1:2024173-2024192 Msa1004520:CDS 40.0%
CCAAACTCAGCAAACAAACA+TGG + chr7_1:2024391-2024410 None:intergenic 40.0%
! CCATGTTTGTTTGCTGAGTT+TGG - chr7_1:2024388-2024407 Msa1004520:CDS 40.0%
GGTTACTGCAGGAAATGAAA+TGG - chr7_1:2024488-2024507 Msa1004520:CDS 40.0%
GCATTGGATGAACTTGCAAA+AGG + chr7_1:2024582-2024601 None:intergenic 40.0%
TCACAAGCTTCTTCTGTTCT+TGG - chr7_1:2024624-2024643 Msa1004520:CDS 40.0%
!! TTTCTAGCTGATGCATACCT+TGG - chr7_1:2024651-2024670 Msa1004520:CDS 40.0%
CTACAATTTATCTCGCGGTA+TGG - chr7_1:2024699-2024718 Msa1004520:CDS 40.0%
ATCTGACAGCTCAAATCGAA+AGG + chr7_1:2024732-2024751 None:intergenic 40.0%
ACATTGATGTGTGATCAACG+TGG - chr7_1:2025129-2025148 Msa1004520:intron 40.0%
! TGGTCACAGTTTTCTTGGTT+TGG + chr7_1:2025505-2025524 None:intergenic 40.0%
! TAGAGGTATGTCATTTGCCA+TGG + chr7_1:2025565-2025584 None:intergenic 40.0%
CCGACATATCTTATCCATGT+TGG - chr7_1:2025653-2025672 Msa1004520:intron 40.0%
GCTCCAAAAGAAGATACACA+TGG + chr7_1:2025903-2025922 None:intergenic 40.0%
AATTGTAGCATTCACAGCAG+TGG - chr7_1:2026010-2026029 Msa1004520:CDS 40.0%
TGGTTCATTGGCAATAGCAA+TGG - chr7_1:2026070-2026089 Msa1004520:exon 40.0%
GGTTCATTGGCAATAGCAAT+GGG - chr7_1:2026071-2026090 Msa1004520:exon 40.0%
GGCAACCTATGTCTATACAA+AGG + chr7_1:2026128-2026147 None:intergenic 40.0%
TTGTATAGACATAGGTTGCC+AGG - chr7_1:2026128-2026147 Msa1004520:intron 40.0%
GGGTAGAAGATCTGCTATAA+AGG - chr7_1:2026253-2026272 Msa1004520:intron 40.0%
!! GGTTCCATTGACTTGGATTA+GGG + chr7_1:2026710-2026729 None:intergenic 40.0%
!! AGGTTCCATTGACTTGGATT+AGG + chr7_1:2026711-2026730 None:intergenic 40.0%
! GTACAAAGGTTCCATTGACT+TGG + chr7_1:2026717-2026736 None:intergenic 40.0%
ATGATTGTGCAAGCTGGAAT+TGG + chr7_1:2026783-2026802 None:intergenic 40.0%
TTAAGCATGATTGTGCAAGC+TGG + chr7_1:2026789-2026808 None:intergenic 40.0%
GCATCTCAGCTTCAAAGAAT+TGG - chr7_1:2026999-2027018 Msa1004520:five_prime_UTR 40.0%
! CCAATGCTTTTCATAGCATC+AGG + chr7_1:2027239-2027258 None:intergenic 40.0%
! CCTGATGCTATGAAAAGCAT+TGG - chr7_1:2027236-2027255 Msa1004520:intron 40.0%
! AGTGTTGTTAAATCTGCCAC+TGG - chr7_1:2027317-2027336 Msa1004520:intron 40.0%
TGAGTCTGAGGATAGAAGGA+TGG - chr7_1:2024091-2024110 Msa1004520:CDS 45.0%
GAGGATAGAAGGATGGCTTT+AGG - chr7_1:2024098-2024117 Msa1004520:CDS 45.0%
TTAGGGCGTGGTTATACAAG+AGG - chr7_1:2024116-2024135 Msa1004520:CDS 45.0%
! GCTTTTATGGTGGTTACTGC+AGG - chr7_1:2024477-2024496 Msa1004520:CDS 45.0%
CAAGCTTCTTCTGTTCTTGG+TGG - chr7_1:2024627-2024646 Msa1004520:CDS 45.0%
ATGCATACCTTGGACGTTAC+TGG - chr7_1:2024661-2024680 Msa1004520:CDS 45.0%
GTATTGTCCAGTAACGTCCA+AGG + chr7_1:2024671-2024690 None:intergenic 45.0%
TGTGATCAACGTGGTTGAAG+AGG - chr7_1:2025138-2025157 Msa1004520:intron 45.0%
GTGATCAACGTGGTTGAAGA+GGG - chr7_1:2025139-2025158 Msa1004520:intron 45.0%
TGATCAACGTGGTTGAAGAG+GGG - chr7_1:2025140-2025159 Msa1004520:intron 45.0%
AGGCAAATTGGTTGTCGGAA+TGG + chr7_1:2025287-2025306 None:intergenic 45.0%
!! GTCATTTGCCATGGTTTTGC+AGG + chr7_1:2025556-2025575 None:intergenic 45.0%
! ATAACAGCATTTGGAGCTGC+AGG - chr7_1:2025598-2025617 Msa1004520:CDS 45.0%
GTCAATGTCTGACACCAACA+TGG + chr7_1:2025670-2025689 None:intergenic 45.0%
GGAAGAATTGACACGGACAA+CGG + chr7_1:2025779-2025798 None:intergenic 45.0%
!!! GGGTTCTGCTTTTGGTTCAT+TGG - chr7_1:2026058-2026077 Msa1004520:exon 45.0%
TATAGACATAGGTTGCCAGG+TGG - chr7_1:2026131-2026150 Msa1004520:intron 45.0%
TACACGAGTCGCTCAAGTAT+TGG - chr7_1:2026163-2026182 Msa1004520:intron 45.0%
TTGGAGCTGAGTAGTTAGCA+AGG + chr7_1:2026327-2026346 None:intergenic 45.0%
ATGGCCCTAATCCAAGTCAA+TGG - chr7_1:2026703-2026722 Msa1004520:intron 45.0%
ACTTCCAGTTACATGCATGC+CGG - chr7_1:2026884-2026903 Msa1004520:five_prime_UTR 45.0%
AAAACCGGCATGCATGTAAC+TGG + chr7_1:2026891-2026910 None:intergenic 45.0%
ACACTTAGGCCTGGAAAAAC+CGG + chr7_1:2026906-2026925 None:intergenic 45.0%
ATGAGGAATACACTTAGGCC+TGG + chr7_1:2026915-2026934 None:intergenic 45.0%
CCAGTGATGCGTTTGAAAAG+TGG + chr7_1:2026966-2026985 None:intergenic 45.0%
!! CCACTTTTCAAACGCATCAC+TGG - chr7_1:2026963-2026982 Msa1004520:five_prime_UTR 45.0%
TCTGCTTATGCTGCATTAGC+TGG - chr7_1:2027260-2027279 Msa1004520:intron 45.0%
!! TCACCAGAAGCACCTCAACA+AGG - chr7_1:2024020-2024039 Msa1004520:CDS 50.0%
!! CACCAGAAGCACCTCAACAA+GGG - chr7_1:2024021-2024040 Msa1004520:CDS 50.0%
ACTGTGAGCCTGCAAAACCA+TGG - chr7_1:2025545-2025564 Msa1004520:CDS 50.0%
!!! TTGGGTTGGGGTTCTGCTTT+TGG - chr7_1:2026050-2026069 Msa1004520:CDS 50.0%
ACTTGAGCGACTCGTGTAAG+AGG + chr7_1:2026161-2026180 None:intergenic 50.0%
! TGAGGTTGTTGGGTTGTACG+AGG - chr7_1:2026226-2026245 Msa1004520:intron 50.0%
! TGTTGGGTTGTACGAGGTTC+CGG - chr7_1:2026232-2026251 Msa1004520:intron 50.0%
!!! ACATGCATGCCGGTTTTTCC+AGG - chr7_1:2026894-2026913 Msa1004520:five_prime_UTR 50.0%
TGAAGCTGAGATGCACCATG+CGG + chr7_1:2026993-2027012 None:intergenic 50.0%
GCTTATGCTGCATTAGCTGG+TGG - chr7_1:2027263-2027282 Msa1004520:intron 50.0%
!! GCTGCATTAGCTGGTGGATT+AGG - chr7_1:2027269-2027288 Msa1004520:intron 50.0%
! GTCCCTTGTTGAGGTGCTTC+TGG + chr7_1:2024026-2024045 None:intergenic 55.0%
GTGCATGGTGTCCCTTGTTG+AGG + chr7_1:2024035-2024054 None:intergenic 55.0%
!! AGAAGGATGGCTTTAGGGCG+TGG - chr7_1:2024104-2024123 Msa1004520:CDS 55.0%
CGTGTAAGAGGACTTCCACC+TGG + chr7_1:2026149-2026168 None:intergenic 55.0%
! GTTGGGTTGTACGAGGTTCC+GGG - chr7_1:2026233-2026252 Msa1004520:intron 55.0%
CGCATCACTGGTCATCCGCA+TGG - chr7_1:2026975-2026994 Msa1004520:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 2023877 2027683 2023877 ID=Msa1004520;Name=Msa1004520
chr7_1 mRNA 2023877 2027683 2023877 ID=Msa1004520-mRNA-1;Parent=Msa1004520;Name=Msa1004520-mRNA-1;_AED=0.10;_eAED=0.10;_QI=592|1|1|1|0.75|0.6|5|121|481
chr7_1 exon 2023877 2024892 2023877 ID=Msa1004520-mRNA-1:exon:809;Parent=Msa1004520-mRNA-1
chr7_1 exon 2025251 2025668 2025251 ID=Msa1004520-mRNA-1:exon:808;Parent=Msa1004520-mRNA-1
chr7_1 exon 2025941 2026103 2025941 ID=Msa1004520-mRNA-1:exon:807;Parent=Msa1004520-mRNA-1
chr7_1 exon 2026845 2027061 2026845 ID=Msa1004520-mRNA-1:exon:806;Parent=Msa1004520-mRNA-1
chr7_1 exon 2027339 2027683 2027339 ID=Msa1004520-mRNA-1:exon:805;Parent=Msa1004520-mRNA-1
chr7_1 five_prime_UTR 2027339 2027683 2027339 ID=Msa1004520-mRNA-1:five_prime_utr;Parent=Msa1004520-mRNA-1
chr7_1 five_prime_UTR 2026845 2027061 2026845 ID=Msa1004520-mRNA-1:five_prime_utr;Parent=Msa1004520-mRNA-1
chr7_1 five_prime_UTR 2026074 2026103 2026074 ID=Msa1004520-mRNA-1:five_prime_utr;Parent=Msa1004520-mRNA-1
chr7_1 CDS 2025941 2026073 2025941 ID=Msa1004520-mRNA-1:cds;Parent=Msa1004520-mRNA-1
chr7_1 CDS 2025251 2025668 2025251 ID=Msa1004520-mRNA-1:cds;Parent=Msa1004520-mRNA-1
chr7_1 CDS 2023998 2024892 2023998 ID=Msa1004520-mRNA-1:cds;Parent=Msa1004520-mRNA-1
chr7_1 three_prime_UTR 2023877 2023997 2023877 ID=Msa1004520-mRNA-1:three_prime_utr;Parent=Msa1004520-mRNA-1
Gene Sequence

>Msa1004520

ATGAGCATTTTTGTGCCAAACCAAGAAAACTGTGACCAATTATCACTTCTATTAGGAAACTGTGAGCCTGCAAAACCATGGCAAATGACATACCTCTACACTGTTCTATACATAACAGCATTTGGAGCTGCAGGTATAAGACCATGTGTATCTTCTTTTGGAGCAGACCAATTCGACGAAAGAAGCAAAAACTACAAATCACATCTTGACAGATTTTTCAACTTGTTCTATCTTTCTGTGACAATTGGTGCAATTGTAGCATTCACAGCAGTGGTCTATGTTCAAATGAAATTGGGTTGGGGTTCTGCTTTTGGTTCATTGGCAATAGCAATGGGAGTATCTAACATGCTTTTCTTTATTGGTACACCTTTGTATAGACATAGGTTGCCAGGTGGAAGTCCTCTTACACGAGTCGCTCAAGTATTGGTTGCCGCTTATCGAAAGAGAAAAGAATCATTTGGTAATAGTGAGGTTGTTGGGTTGTACGAGGTTCCGGGTAGAAGATCTGCTATAAAGGGTAGTCAGAAAATTGCTCATACTGATGATTTCAGGTTTCTTGACAAAGCAGCTCTACAATTGAAACAAGATGGCCCTAATCCAAGTCAATGGAACCTTTGTACAGTAACACAAGTAGAAGAAGTGAAGATCCTTATAAAACTAATTCCAATTCCAGCTTGCACAATCATGCTTAATGTAATCTTAACCGAATTTCTAACTCTCTCAGTCCAACAAGCATATACCTTAAACACTCACTTAGGTAAATTAAAACTTCCAGTTACATGCATGCCGGTTTTTCCAGGCCTAAGTGTATTCCTCATATTAGCTCTCTATTACCAAACTTTTGTACCACTTTTCAAACGCATCACTGGTCATCCGCATGGTGCATCTCAGCTTCAAAGAATTGGAATTGGTTTAGGAGTTTCGATTTTATCAGTTGCTTGGGCTGCAATCTTCGAAAAGTATAGAAGAAACTATGCGATAAAAAATGAATTTGAAGCTAGTTTCTTAACACCAATGCCAAATTTAAGTGCTTATTGGTTATTGATTCAATATTGCTTAATTGGTGTAGCTGAAGTTTTTTGCATTGTTGGTTTGTTGGAATTTTTATATGAAGAAGCACCTGATGCTATGAAAAGCATTGGTTCTGCTTATGCTGCATTAGCTGGTGGATTAGGTTGTTTTTTTGCAACATTTATAAATAGTGTTGTTAAATCTGCCACTGGAAATTCAGATAAAAGACAAGAATCTTGGTTGTCTCAAAACATCAACACTGGCAAGTTTGATTACTTTTACTGGGTTCTTACAGCTTTAAGCTTAGTCAACTTCTGTGTCTTCTTATATTCAGCACATAGATACAAATACAGGACACAACATGTTTATGAGATGGAGAACATTAAACTTGATAATTTGGCAAACAATGGGAGCTCTACAACAGTTTCTTTCTAG
Protein sequence

>Msa1004520

MSIFVPNQENCDQLSLLLGNCEPAKPWQMTYLYTVLYITAFGAAGIRPCVSSFGADQFDERSKNYKSHLDRFFNLFYLSVTIGAIVAFTAVVYVQMKLGWGSAFGSLAIAMGVSNMLFFIGTPLYRHRLPGGSPLTRVAQVLVAAYRKRKESFGNSEVVGLYEVPGRRSAIKGSQKIAHTDDFRFLDKAALQLKQDGPNPSQWNLCTVTQVEEVKILIKLIPIPACTIMLNVILTEFLTLSVQQAYTLNTHLGKLKLPVTCMPVFPGLSVFLILALYYQTFVPLFKRITGHPHGASQLQRIGIGLGVSILSVAWAAIFEKYRRNYAIKNEFEASFLTPMPNLSAYWLLIQYCLIGVAEVFCIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFFATFINSVVKSATGNSDKRQESWLSQNINTGKFDYFYWVLTALSLVNFCVFLYSAHRYKYRTQHVYEMENIKLDNLANNGSSTTVSF*