Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0629850 | Msa0883370 | 0.826744 | 2.293491e-54 | -8.615850e-47 |
| Msa0629880 | Msa0883370 | 0.803409 | 3.535002e-49 | -8.615850e-47 |
| Msa0754180 | Msa0883370 | 0.805569 | 1.252848e-49 | -8.615850e-47 |
| Msa0784580 | Msa0883370 | 0.836480 | 9.127580e-57 | -8.615850e-47 |
| Msa0818010 | Msa0883370 | 0.853385 | 2.474406e-61 | -8.615850e-47 |
| Msa0818030 | Msa0883370 | 0.860627 | 1.810230e-63 | -8.615850e-47 |
| Msa0193010 | Msa0883370 | 0.813544 | 2.426031e-51 | -8.615850e-47 |
| Msa0211680 | Msa0883370 | 0.827402 | 1.595463e-54 | -8.615850e-47 |
| Msa0235190 | Msa0883370 | 0.865984 | 3.970633e-65 | -8.615850e-47 |
| Msa0458700 | Msa0883370 | 0.819139 | 1.358529e-52 | -8.615850e-47 |
| Msa0273560 | Msa0883370 | 0.801983 | 6.959591e-49 | -8.615850e-47 |
| Msa0273570 | Msa0883370 | 0.843590 | 1.275994e-58 | -8.615850e-47 |
| Msa0273580 | Msa0883370 | 0.835627 | 1.503161e-56 | -8.615850e-47 |
| Msa0313780 | Msa0883370 | 0.818347 | 2.055726e-52 | -8.615850e-47 |
| Msa0313920 | Msa0883370 | 0.812688 | 3.738568e-51 | -8.615850e-47 |
| Msa0314160 | Msa0883370 | 0.814855 | 1.245430e-51 | -8.615850e-47 |
| Msa0506000 | Msa0883370 | 0.804945 | 1.692922e-49 | -8.615850e-47 |
| Msa0533290 | Msa0883370 | 0.811816 | 5.793199e-51 | -8.615850e-47 |
| Msa0533430 | Msa0883370 | 0.827013 | 1.977441e-54 | -8.615850e-47 |
| Msa0580610 | Msa0883370 | 0.841223 | 5.411479e-58 | -8.615850e-47 |
| Msa0843280 | Msa0883370 | 0.802630 | 5.120451e-49 | -8.615850e-47 |
| Msa0858360 | Msa0883370 | 0.830620 | 2.650190e-55 | -8.615850e-47 |
| Msa0865390 | Msa0883370 | 0.803788 | 2.949699e-49 | -8.615850e-47 |
| Msa0883370 | Msa0912500 | 0.846845 | 1.681522e-59 | -8.615850e-47 |
| Msa0883370 | Msa0965750 | 0.820796 | 5.674695e-53 | -8.615850e-47 |
| Msa0883370 | Msa1006250 | 0.836024 | 1.192133e-56 | -8.615850e-47 |
| Msa0883370 | Msa1027240 | 0.814975 | 1.171648e-51 | -8.615850e-47 |
| Msa0883370 | Msa1037710 | 0.800143 | 1.654515e-48 | -8.615850e-47 |
| Msa0883370 | Msa1107800 | 0.806392 | 8.412508e-50 | -8.615850e-47 |
| Msa0883370 | Msa1275770 | 0.813121 | 3.003895e-51 | -8.615850e-47 |
| Msa0883370 | Msa1287560 | 0.819683 | 1.021040e-52 | -8.615850e-47 |
| Msa0883370 | Msa1288000 | 0.816844 | 4.485749e-52 | -8.615850e-47 |
| Msa0883370 | Msa1324780 | 0.813784 | 2.147889e-51 | -8.615850e-47 |
| Msa0883370 | Msa1359820 | 0.822804 | 1.947276e-53 | -8.615850e-47 |
| Msa0883370 | Msa1423990 | 0.840463 | 8.565637e-58 | -8.615850e-47 |
| Msa0883370 | Msa1444370 | 0.832616 | 8.539055e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGAATTGCTTATATA+AGG | 0.243412 | 6_1:-34371408 | Msa0883370:CDS |
| TAATAGGGCCAATGCTTCTT+TGG | 0.262420 | 6_1:-34371435 | Msa0883370:CDS |
| CAATGCTTCTTTGGAGATCT+TGG | 0.288896 | 6_1:-34371426 | Msa0883370:CDS |
| TCTAGTCTTTGAGCCACATT+TGG | 0.369283 | 6_1:+34371326 | None:intergenic |
| GATAGACTCTATATATGTAT+TGG | 0.463536 | 6_1:-34371371 | Msa0883370:CDS |
| TTGGGGATAGGCTATTTCTC+AGG | 0.469367 | 6_1:-34371469 | Msa0883370:CDS |
| TCAGAAATTCATGGTGAAGA+TGG | 0.503426 | 6_1:-34371526 | None:intergenic |
| AATGTGGCTCAAAGACTAGA+AGG | 0.528204 | 6_1:-34371323 | Msa0883370:intron |
| CAAGATCTCCAAAGAAGCAT+TGG | 0.540803 | 6_1:+34371427 | None:intergenic |
| ATGTGACAAGGTTCCAAATG+TGG | 0.589849 | 6_1:-34371339 | Msa0883370:CDS |
| TGGTAGTAGTCAATGTGACA+AGG | 0.683937 | 6_1:-34371351 | Msa0883370:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAATCCATTAATTATT+TGG | - | chr6_1:34371225-34371244 | Msa0883370:intron | 10.0% |
| !!! | ATTAGTTTTATAGTTTATTA+AGG | + | chr6_1:34371500-34371519 | None:intergenic | 10.0% |
| !! | AATTAAATCCATTAATTATT+TGG | - | chr6_1:34371225-34371244 | Msa0883370:intron | 10.0% |
| !!! | ATTAGTTTTATAGTTTATTA+AGG | + | chr6_1:34371500-34371519 | None:intergenic | 10.0% |
| !! | TAGTACTCCAATAATTAATT+AGG | - | chr6_1:34371042-34371061 | Msa0883370:intron | 20.0% |
| !! | TTAGTCTCCTAATTAATTAT+TGG | + | chr6_1:34371052-34371071 | None:intergenic | 20.0% |
| !! | TAAGAACTAACCTATAAAAA+TGG | + | chr6_1:34371138-34371157 | None:intergenic | 20.0% |
| !! | ATTCACAACCAAATAATTAA+TGG | + | chr6_1:34371236-34371255 | None:intergenic | 20.0% |
| !!! | TGTTTTTCTATTACTCAAAT+AGG | - | chr6_1:34371449-34371468 | Msa0883370:CDS | 20.0% |
| !!! | GTTTTTCTATTACTCAAATA+GGG | - | chr6_1:34371450-34371469 | Msa0883370:CDS | 20.0% |
| !! | TAGTACTCCAATAATTAATT+AGG | - | chr6_1:34371042-34371061 | Msa0883370:intron | 20.0% |
| !! | TTAGTCTCCTAATTAATTAT+TGG | + | chr6_1:34371052-34371071 | None:intergenic | 20.0% |
| !! | TAAGAACTAACCTATAAAAA+TGG | + | chr6_1:34371138-34371157 | None:intergenic | 20.0% |
| !! | ATTCACAACCAAATAATTAA+TGG | + | chr6_1:34371236-34371255 | None:intergenic | 20.0% |
| !!! | TGTTTTTCTATTACTCAAAT+AGG | - | chr6_1:34371449-34371468 | Msa0883370:CDS | 20.0% |
| !!! | GTTTTTCTATTACTCAAATA+GGG | - | chr6_1:34371450-34371469 | Msa0883370:CDS | 20.0% |
| ! | GATAGACTCTATATATGTAT+TGG | - | chr6_1:34370895-34370914 | Msa0883370:intron | 25.0% |
| !!! | CACTAAAATGCCATTTTTAT+AGG | - | chr6_1:34371125-34371144 | Msa0883370:intron | 25.0% |
| ! | GATAGACTCTATATATGTAT+TGG | - | chr6_1:34370895-34370914 | Msa0883370:intron | 25.0% |
| !!! | CACTAAAATGCCATTTTTAT+AGG | - | chr6_1:34371125-34371144 | Msa0883370:intron | 25.0% |
| CTTGGAGAATTGCTTATATA+AGG | - | chr6_1:34370858-34370877 | Msa0883370:intron | 30.0% | |
| ! | AGACTAATTTGTATGCACTT+AGG | - | chr6_1:34371065-34371084 | Msa0883370:intron | 30.0% |
| !! | GCATTTTAGTGTGATTTGAT+TGG | + | chr6_1:34371116-34371135 | None:intergenic | 30.0% |
| CTTGGAGAATTGCTTATATA+AGG | - | chr6_1:34370858-34370877 | Msa0883370:intron | 30.0% | |
| ! | AGACTAATTTGTATGCACTT+AGG | - | chr6_1:34371065-34371084 | Msa0883370:intron | 30.0% |
| !! | GCATTTTAGTGTGATTTGAT+TGG | + | chr6_1:34371116-34371135 | None:intergenic | 30.0% |
| AGCACATGTAAAATCCTTCA+TGG | + | chr6_1:34370778-34370797 | None:intergenic | 35.0% | |
| ! | TGAAGGATTTTACATGTGCT+TGG | - | chr6_1:34370778-34370797 | Msa0883370:intron | 35.0% |
| ! | GAAGGATTTTACATGTGCTT+GGG | - | chr6_1:34370779-34370798 | Msa0883370:intron | 35.0% |
| ! | AAGGATTTTACATGTGCTTG+GGG | - | chr6_1:34370780-34370799 | Msa0883370:intron | 35.0% |
| !!! | TAGGCTATTTCTCAGGTTTT+TGG | - | chr6_1:34370804-34370823 | Msa0883370:intron | 35.0% |
| !!! | AGGCTATTTCTCAGGTTTTT+GGG | - | chr6_1:34370805-34370824 | Msa0883370:intron | 35.0% |
| GATTGGACGTATGTCTAAAA+AGG | + | chr6_1:34371099-34371118 | None:intergenic | 35.0% | |
| AGCACATGTAAAATCCTTCA+TGG | + | chr6_1:34370778-34370797 | None:intergenic | 35.0% | |
| ! | TGAAGGATTTTACATGTGCT+TGG | - | chr6_1:34370778-34370797 | Msa0883370:intron | 35.0% |
| ! | GAAGGATTTTACATGTGCTT+GGG | - | chr6_1:34370779-34370798 | Msa0883370:intron | 35.0% |
| ! | AAGGATTTTACATGTGCTTG+GGG | - | chr6_1:34370780-34370799 | Msa0883370:intron | 35.0% |
| !!! | TAGGCTATTTCTCAGGTTTT+TGG | - | chr6_1:34370804-34370823 | Msa0883370:intron | 35.0% |
| !!! | AGGCTATTTCTCAGGTTTTT+GGG | - | chr6_1:34370805-34370824 | Msa0883370:intron | 35.0% |
| GATTGGACGTATGTCTAAAA+AGG | + | chr6_1:34371099-34371118 | None:intergenic | 35.0% | |
| ! | GGCAATTCAGTTTTCCATGA+AGG | - | chr6_1:34370761-34370780 | Msa0883370:intron | 40.0% |
| ! | TTTTACATGTGCTTGGGGAT+AGG | - | chr6_1:34370785-34370804 | Msa0883370:intron | 40.0% |
| !!! | GGCTATTTCTCAGGTTTTTG+GGG | - | chr6_1:34370806-34370825 | Msa0883370:intron | 40.0% |
| !!! | TCAGGTTTTTGGGGCTTAAT+AGG | - | chr6_1:34370815-34370834 | Msa0883370:intron | 40.0% |
| !!! | CAGGTTTTTGGGGCTTAATA+GGG | - | chr6_1:34370816-34370835 | Msa0883370:intron | 40.0% |
| TAATAGGGCCAATGCTTCTT+TGG | - | chr6_1:34370831-34370850 | Msa0883370:intron | 40.0% | |
| ! | CAAGATCTCCAAAGAAGCAT+TGG | + | chr6_1:34370842-34370861 | None:intergenic | 40.0% |
| ! | CAATGCTTCTTTGGAGATCT+TGG | - | chr6_1:34370840-34370859 | Msa0883370:intron | 40.0% |
| TGGTAGTAGTCAATGTGACA+AGG | - | chr6_1:34370915-34370934 | Msa0883370:intron | 40.0% | |
| ATGTGACAAGGTTCCAAATG+TGG | - | chr6_1:34370927-34370946 | Msa0883370:intron | 40.0% | |
| ! | TCTAGTCTTTGAGCCACATT+TGG | + | chr6_1:34370943-34370962 | None:intergenic | 40.0% |
| AATGTGGCTCAAAGACTAGA+AGG | - | chr6_1:34370943-34370962 | Msa0883370:intron | 40.0% | |
| ! | AACTCTCAATGCTTGCTACT+AGG | + | chr6_1:34371282-34371301 | None:intergenic | 40.0% |
| ! | GGCAATTCAGTTTTCCATGA+AGG | - | chr6_1:34370761-34370780 | Msa0883370:intron | 40.0% |
| ! | TTTTACATGTGCTTGGGGAT+AGG | - | chr6_1:34370785-34370804 | Msa0883370:intron | 40.0% |
| !!! | GGCTATTTCTCAGGTTTTTG+GGG | - | chr6_1:34370806-34370825 | Msa0883370:intron | 40.0% |
| !!! | TCAGGTTTTTGGGGCTTAAT+AGG | - | chr6_1:34370815-34370834 | Msa0883370:intron | 40.0% |
| !!! | CAGGTTTTTGGGGCTTAATA+GGG | - | chr6_1:34370816-34370835 | Msa0883370:intron | 40.0% |
| TAATAGGGCCAATGCTTCTT+TGG | - | chr6_1:34370831-34370850 | Msa0883370:intron | 40.0% | |
| ! | CAAGATCTCCAAAGAAGCAT+TGG | + | chr6_1:34370842-34370861 | None:intergenic | 40.0% |
| ! | CAATGCTTCTTTGGAGATCT+TGG | - | chr6_1:34370840-34370859 | Msa0883370:intron | 40.0% |
| TGGTAGTAGTCAATGTGACA+AGG | - | chr6_1:34370915-34370934 | Msa0883370:intron | 40.0% | |
| ATGTGACAAGGTTCCAAATG+TGG | - | chr6_1:34370927-34370946 | Msa0883370:intron | 40.0% | |
| ! | TCTAGTCTTTGAGCCACATT+TGG | + | chr6_1:34370943-34370962 | None:intergenic | 40.0% |
| AATGTGGCTCAAAGACTAGA+AGG | - | chr6_1:34370943-34370962 | Msa0883370:intron | 40.0% | |
| ! | AACTCTCAATGCTTGCTACT+AGG | + | chr6_1:34371282-34371301 | None:intergenic | 40.0% |
| !! | TTGGGGATAGGCTATTTCTC+AGG | - | chr6_1:34370797-34370816 | Msa0883370:intron | 45.0% |
| !! | TAGCAAGCATTGAGAGTTGC+TGG | - | chr6_1:34371284-34371303 | Msa0883370:intron | 45.0% |
| !! | TTGGGGATAGGCTATTTCTC+AGG | - | chr6_1:34370797-34370816 | Msa0883370:intron | 45.0% |
| !! | TAGCAAGCATTGAGAGTTGC+TGG | - | chr6_1:34371284-34371303 | Msa0883370:intron | 45.0% |
| ! | AGCATTGAGAGTTGCTGGAC+TGG | - | chr6_1:34371289-34371308 | Msa0883370:intron | 50.0% |
| ! | TTGAGAGTTGCTGGACTGGA+AGG | - | chr6_1:34371293-34371312 | Msa0883370:intron | 50.0% |
| ! | TGAGAGTTGCTGGACTGGAA+GGG | - | chr6_1:34371294-34371313 | Msa0883370:intron | 50.0% |
| ! | AGCATTGAGAGTTGCTGGAC+TGG | - | chr6_1:34371289-34371308 | Msa0883370:intron | 50.0% |
| ! | TTGAGAGTTGCTGGACTGGA+AGG | - | chr6_1:34371293-34371312 | Msa0883370:intron | 50.0% |
| ! | TGAGAGTTGCTGGACTGGAA+GGG | - | chr6_1:34371294-34371313 | Msa0883370:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 34370750 | 34371538 | 34370750 | ID=Msa0883370;Name=Msa0883370 |
| chr6_1 | mRNA | 34370750 | 34371538 | 34370750 | ID=Msa0883370-mRNA-1;Parent=Msa0883370;Name=Msa0883370-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.66|0|0|3|0|69 |
| chr6_1 | exon | 34371519 | 34371538 | 34371519 | ID=Msa0883370-mRNA-1:exon:8362;Parent=Msa0883370-mRNA-1 |
| chr6_1 | exon | 34371324 | 34371505 | 34371324 | ID=Msa0883370-mRNA-1:exon:8361;Parent=Msa0883370-mRNA-1 |
| chr6_1 | exon | 34370750 | 34370757 | 34370750 | ID=Msa0883370-mRNA-1:exon:8360;Parent=Msa0883370-mRNA-1 |
| chr6_1 | CDS | 34371519 | 34371538 | 34371519 | ID=Msa0883370-mRNA-1:cds;Parent=Msa0883370-mRNA-1 |
| chr6_1 | CDS | 34371324 | 34371505 | 34371324 | ID=Msa0883370-mRNA-1:cds;Parent=Msa0883370-mRNA-1 |
| chr6_1 | CDS | 34370750 | 34370757 | 34370750 | ID=Msa0883370-mRNA-1:cds;Parent=Msa0883370-mRNA-1 |
| Gene Sequence |
| Protein sequence |
