AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1275770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1275770 MtrunA17_Chr8g0342841 88.073 109 8 2 1 109 1 104 2.80e-62 184
Msa1275770 MtrunA17_Chr8g0342831 88.073 109 8 2 1 109 1 104 2.80e-62 184
Msa1275770 MtrunA17_Chr2g0329951 64.815 108 31 4 1 107 1 102 1.87e-38 124
Msa1275770 MtrunA17_Chr4g0071001 52.066 121 40 4 2 108 3 119 1.02e-29 103
Msa1275770 MtrunA17_Chr3g0135901 54.717 106 42 3 4 108 6 106 1.48e-18 74.3
Msa1275770 MtrunA17_Chr7g0269811 65.217 46 16 0 63 108 42 87 6.02e-17 69.7
Msa1275770 MtrunA17_Chr1g0195031 54.000 50 23 0 59 108 53 102 3.65e-16 68.2
Msa1275770 MtrunA17_Chr1g0151181 72.222 36 10 0 68 103 74 109 6.74e-13 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1275770 AT5G11970.1 43.810 105 49 3 4 108 6 100 8.09e-20 77.8
Msa1275770 AT2G19460.1 43.590 117 51 6 2 108 3 114 1.29e-18 75.1
Msa1275770 AT2G19460.2 43.478 115 54 5 2 108 55 166 2.92e-18 75.5
Msa1275770 AT3G13910.1 59.016 61 22 1 48 105 39 99 4.32e-16 68.2
Msa1275770 AT3G13910.2 56.250 64 25 1 48 108 39 102 5.11e-16 68.6
Msa1275770 AT1G72720.1 38.095 126 59 6 3 109 2 127 9.23e-13 60.5
Msa1275770 AT2G47480.1 45.000 60 33 0 49 108 47 106 2.95e-12 58.5
Msa1275770 AT3G05725.1 35.780 109 53 4 16 108 13 120 7.80e-12 57.8

Find 10 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCAATTATCACCATAAT+TGG 0.252461 8_3:+9928881 None:intergenic
ACTTTGATCCTTGCATGATT+TGG 0.271027 8_3:+9929185 None:intergenic
AGAACACGTGCAGCCAATTA+TGG 0.311974 8_3:-9928894 Msa1275770:CDS
CTCAGATCTTGCATGCTTGA+TGG 0.343477 8_3:+9929126 None:intergenic
CAGAGAAAGAAAAGAGTAGC+TGG 0.442245 8_3:-9928982 Msa1275770:CDS
TTGCAGATTGAAAGCTATAG+TGG 0.472998 8_3:-9929162 Msa1275770:CDS
TATAAAGTATATGATGTTGA+AGG 0.535495 8_3:-9928958 Msa1275770:CDS
TGTTATAGTACAAATTATGA+TGG 0.558074 8_3:-9929099 Msa1275770:CDS
CAGATTGAAAGCTATAGTGG+TGG 0.602253 8_3:-9929159 Msa1275770:CDS
ATTCAGATCCAAATCATGCA+AGG 0.617513 8_3:-9929193 Msa1275770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTATAGTACAAATTATGA+TGG - chr8_3:9928990-9929009 Msa1275770:CDS 20.0%
!! TATAAAGTATATGATGTTGA+AGG - chr8_3:9929131-9929150 Msa1275770:CDS 20.0%
!!! ATCAGATTTTGATCAAAACA+AGG - chr8_3:9929019-9929038 Msa1275770:CDS 25.0%
! ATGTTGAAGGAAAAATGAAA+GGG - chr8_3:9929144-9929163 Msa1275770:CDS 25.0%
! TTAGCAAAGCATCAAAAAGT+TGG - chr8_3:9929063-9929082 Msa1275770:CDS 30.0%
GATGTTGAAGGAAAAATGAA+AGG - chr8_3:9929143-9929162 Msa1275770:CDS 30.0%
!! GGTCTTTTAAGAAGAGTTTA+AGG - chr8_3:9929165-9929184 Msa1275770:CDS 30.0%
!! CTTTTAAGAAGAGTTTAAGG+TGG - chr8_3:9929168-9929187 Msa1275770:CDS 30.0%
ATTCAGATCCAAATCATGCA+AGG - chr8_3:9928896-9928915 Msa1275770:CDS 35.0%
ACTTTGATCCTTGCATGATT+TGG + chr8_3:9928907-9928926 None:intergenic 35.0%
TTGCAGATTGAAAGCTATAG+TGG - chr8_3:9928927-9928946 Msa1275770:CDS 35.0%
CAGATTGAAAGCTATAGTGG+TGG - chr8_3:9928930-9928949 Msa1275770:CDS 40.0%
! CTTTTCTTTCTCTGCAACTC+TGG + chr8_3:9929101-9929120 None:intergenic 40.0%
CAGAGAAAGAAAAGAGTAGC+TGG - chr8_3:9929107-9929126 Msa1275770:CDS 40.0%
CTCAGATCTTGCATGCTTGA+TGG + chr8_3:9928966-9928985 None:intergenic 45.0%
AGAACACGTGCAGCCAATTA+TGG - chr8_3:9929195-9929214 Msa1275770:CDS 45.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 9928891 9929220 9928891 ID=Msa1275770;Name=Msa1275770
chr8_3 mRNA 9928891 9929220 9928891 ID=Msa1275770-mRNA-1;Parent=Msa1275770;Name=Msa1275770-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|109
chr8_3 exon 9928891 9929220 9928891 ID=Msa1275770-mRNA-1:exon:4142;Parent=Msa1275770-mRNA-1
chr8_3 CDS 9928891 9929220 9928891 ID=Msa1275770-mRNA-1:cds;Parent=Msa1275770-mRNA-1
Gene Sequence

>Msa1275770

ATGGAATTCAGATCCAAATCATGCAAGGATCAAAGTTTGCAGATTGAAAGCTATAGTGGTGGAAGAGTAGCTCCATCAAGCATGCAAGATCTGAGACGATGTTATAGTACAAATTATGATGGTAATTCATCAGATTTTGATCAAAACAAGGTAGAAAAAAGTAAAAGTAAATTTAGCAAAGCATCAAAAAGTTGGAGCTTCAATGATCCAGAGTTGCAGAGAAAGAAAAGAGTAGCTGGATATAAAGTATATGATGTTGAAGGAAAAATGAAAGGGTCTTTTAAGAAGAGTTTAAGGTGGATCAAGAACACGTGCAGCCAATTATGGTGA

Protein sequence

>Msa1275770

MEFRSKSCKDQSLQIESYSGGRVAPSSMQDLRRCYSTNYDGNSSDFDQNKVEKSKSKFSKASKSWSFNDPELQRKKRVAGYKVYDVEGKMKGSFKKSLRWIKNTCSQLW*