Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076450 | sp|I1M2U5|NAMT1_SOYBN | 51.429 | 70 | 33 | 1 | 1 | 70 | 255 | 323 | 1.94e-19 | 82.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076450 | A0A1S2YDS4 | 54.286 | 70 | 31 | 1 | 1 | 70 | 261 | 329 | 1.56e-20 | 92.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0801760 | Msa1076450 | 0.822777 | 1.975951e-53 | -8.615850e-47 |
| Msa0480980 | Msa1076450 | 0.802839 | 4.636190e-49 | -8.615850e-47 |
| Msa1042710 | Msa1076450 | 0.832692 | 8.177238e-56 | -8.615850e-47 |
| Msa1069310 | Msa1076450 | 0.805204 | 1.494758e-49 | -8.615850e-47 |
| Msa1076450 | Msa1076460 | 0.862181 | 6.077008e-64 | -8.615850e-47 |
| Msa1076450 | Msa1076480 | 0.858286 | 9.143976e-63 | -8.615850e-47 |
| Msa1076450 | Msa1083410 | 0.810205 | 1.294110e-50 | -8.615850e-47 |
| Msa1076450 | Msa1098560 | 0.804327 | 2.278038e-49 | -8.615850e-47 |
| Msa1076450 | Msa1102140 | 0.800815 | 1.207590e-48 | -8.615850e-47 |
| Msa1076450 | Msa1122660 | 0.811122 | 8.196292e-51 | -8.615850e-47 |
| Msa1076450 | Msa1128160 | 0.821301 | 4.342397e-53 | -8.615850e-47 |
| Msa1076450 | Msa1165360 | 0.820808 | 5.639666e-53 | -8.615850e-47 |
| Msa1076450 | Msa1177060 | 0.802274 | 6.064376e-49 | -8.615850e-47 |
| Msa1076450 | Msa1222970 | 0.829539 | 4.864664e-55 | -8.615850e-47 |
| Msa1076450 | Msa1228210 | 0.816796 | 4.598067e-52 | -8.615850e-47 |
| Msa1076450 | Msa1242230 | 0.822098 | 2.841220e-53 | -8.615850e-47 |
| Msa1076450 | Msa1252390 | 0.822501 | 2.289900e-53 | -8.615850e-47 |
| Msa1076450 | Msa1264020 | 0.805347 | 1.394531e-49 | -8.615850e-47 |
| Msa1076450 | Msa1271280 | 0.842614 | 2.322176e-58 | -8.615850e-47 |
| Msa1076450 | Msa1283860 | 0.802091 | 6.611507e-49 | -8.615850e-47 |
| Msa1076450 | Msa1284260 | 0.807636 | 4.588357e-50 | -8.615850e-47 |
| Msa1076450 | Msa1322860 | 0.817584 | 3.057412e-52 | -8.615850e-47 |
| Msa1076450 | Msa1324780 | 0.813613 | 2.342261e-51 | -8.615850e-47 |
| Msa1076450 | Msa1327050 | 0.805781 | 1.131041e-49 | -8.615850e-47 |
| Msa1076450 | Msa1367080 | 0.824388 | 8.293542e-54 | -8.615850e-47 |
| Msa1076450 | Msa1378760 | 0.816148 | 6.419210e-52 | -8.615850e-47 |
| Msa1076450 | Msa1411070 | 0.844368 | 7.893838e-59 | -8.615850e-47 |
| Msa0269360 | Msa1076450 | 0.806589 | 7.642305e-50 | -8.615850e-47 |
| Msa0533070 | Msa1076450 | 0.816407 | 5.618332e-52 | -8.615850e-47 |
| Msa0581760 | Msa1076450 | 0.813730 | 2.207198e-51 | -8.615850e-47 |
| Msa0845590 | Msa1076450 | -0.802838 | 4.638375e-49 | -8.615850e-47 |
| Msa0853540 | Msa1076450 | 0.818475 | 1.922155e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076450 | MtrunA17_Chr3g0130061 | 51.471 | 68 | 32 | 1 | 3 | 70 | 260 | 326 | 7.83e-22 | 86.3 |
| Msa1076450 | MtrunA17_Chr3g0130011 | 47.059 | 68 | 35 | 1 | 3 | 70 | 260 | 326 | 8.55e-18 | 75.1 |
| Msa1076450 | MtrunA17_Chr2g0314271 | 47.143 | 70 | 36 | 1 | 1 | 70 | 254 | 322 | 8.99e-18 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1076450 | AT3G53140.1 | 50.000 | 70 | 34 | 1 | 1 | 70 | 259 | 327 | 1.56e-19 | 80.1 |
Find 18 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACTTGCAGTGGTTGTGTT+TGG | 0.298953 | 7_2:-74979018 | Msa1076450:intron |
| CACTTGCAGTGGTTGTGTTT+GGG | 0.301970 | 7_2:-74979017 | Msa1076450:intron |
| GAATTGCCATCGCACTCTTC+CGG | 0.392975 | 7_2:-74978958 | Msa1076450:CDS |
| TATAATTTGTGATCCTGTGC+TGG | 0.505912 | 7_2:-74978922 | Msa1076450:CDS |
| ACCAGGTCAAAGGCTTTACT+AGG | 0.515856 | 7_2:-74978879 | Msa1076450:CDS |
| GTCTTCATGATGACCATGTA+CGG | 0.525468 | 7_2:-74978849 | Msa1076450:CDS |
| TCCGTCACCTCTGCCAGCAC+AGG | 0.529202 | 7_2:+74978909 | None:intergenic |
| TCCTGTGCTGGCAGAGGTGA+CGG | 0.530070 | 7_2:-74978910 | Msa1076450:CDS |
| GGACGAAAGCACCAGGTCAA+AGG | 0.536548 | 7_2:-74978889 | Msa1076450:CDS |
| ATTATAAGTTTGTCATGCGC+CGG | 0.544530 | 7_2:+74978939 | None:intergenic |
| AGGTGACGGACGAAAGCACC+AGG | 0.581942 | 7_2:-74978896 | Msa1076450:CDS |
| ACCTAGTAAAGCCTTTGACC+TGG | 0.594855 | 7_2:+74978878 | None:intergenic |
| TTGTGATCCTGTGCTGGCAG+AGG | 0.602955 | 7_2:-74978916 | Msa1076450:CDS |
| TCTTCATGATGACCATGTAC+GGG | 0.603036 | 7_2:-74978848 | Msa1076450:CDS |
| AGGTCAAAGGCTTTACTAGG+TGG | 0.619777 | 7_2:-74978876 | Msa1076450:CDS |
| GGTCAAAGGCTTTACTAGGT+GGG | 0.619833 | 7_2:-74978875 | Msa1076450:CDS |
| AATGTAGTGCAAGTATGATG+AGG | 0.641930 | 7_2:-74978980 | Msa1076450:CDS |
| CATGCGCCGGAAGAGTGCGA+TGG | 0.652044 | 7_2:+74978952 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATTTGCAAGTATATA+TGG | + | chr7_2:74978852-74978871 | None:intergenic | 15.0% |
| !! | CATAATCATATTGCATATAT+AGG | - | chr7_2:74978890-74978909 | Msa1076450:CDS | 20.0% |
| !!! | ACGTTTTTAATTCAATTAGA+GGG | + | chr7_2:74978996-74979015 | None:intergenic | 20.0% |
| !!! | AACGTTTTTAATTCAATTAG+AGG | + | chr7_2:74978997-74979016 | None:intergenic | 20.0% |
| !!! | TTTTAATTCAATTAGAGGGT+GGG | + | chr7_2:74978992-74979011 | None:intergenic | 25.0% |
| !!! | TTTTTAATTCAATTAGAGGG+TGG | + | chr7_2:74978993-74979012 | None:intergenic | 25.0% |
| !! | GATGAATTTTGACCTTTATC+AGG | + | chr7_2:74978970-74978989 | None:intergenic | 30.0% |
| AATTAATATTCCACTTGCAG+TGG | - | chr7_2:74979055-74979074 | Msa1076450:intron | 30.0% | |
| TATATAGGCATAACCCATGT+TGG | - | chr7_2:74978905-74978924 | Msa1076450:CDS | 35.0% | |
| AATGTAGTGCAAGTATGATG+AGG | - | chr7_2:74979103-74979122 | Msa1076450:intron | 35.0% | |
| ATTATAAGTTTGTCATGCGC+CGG | + | chr7_2:74979147-74979166 | None:intergenic | 35.0% | |
| ! | TATAATTTGTGATCCTGTGC+TGG | - | chr7_2:74979161-74979180 | Msa1076450:intron | 35.0% |
| GTCTTCATGATGACCATGTA+CGG | - | chr7_2:74979234-74979253 | Msa1076450:intron | 40.0% | |
| TCTTCATGATGACCATGTAC+GGG | - | chr7_2:74979235-74979254 | Msa1076450:intron | 40.0% | |
| !! | TTTTGCTTTTGACCCGTACA+TGG | + | chr7_2:74979250-74979269 | None:intergenic | 40.0% |
| ATAGGCATAACCCATGTTGG+TGG | - | chr7_2:74978908-74978927 | Msa1076450:CDS | 45.0% | |
| TGGAGACGCTATCCTGATAA+AGG | - | chr7_2:74978955-74978974 | Msa1076450:CDS | 45.0% | |
| CACTTGCAGTGGTTGTGTTT+GGG | - | chr7_2:74979066-74979085 | Msa1076450:intron | 45.0% | |
| ACCAGGTCAAAGGCTTTACT+AGG | - | chr7_2:74979204-74979223 | Msa1076450:intron | 45.0% | |
| ACCTAGTAAAGCCTTTGACC+TGG | + | chr7_2:74979208-74979227 | None:intergenic | 45.0% | |
| AGGTCAAAGGCTTTACTAGG+TGG | - | chr7_2:74979207-74979226 | Msa1076450:intron | 45.0% | |
| GGTCAAAGGCTTTACTAGGT+GGG | - | chr7_2:74979208-74979227 | Msa1076450:intron | 45.0% | |
| AGTGGCATCACCACCAACAT+GGG | + | chr7_2:74978921-74978940 | None:intergenic | 50.0% | |
| TCCACGAGGAACAAACTCAG+TGG | + | chr7_2:74978939-74978958 | None:intergenic | 50.0% | |
| CCAAACACAACCACTGCAAG+TGG | + | chr7_2:74979068-74979087 | None:intergenic | 50.0% | |
| CCACTTGCAGTGGTTGTGTT+TGG | - | chr7_2:74979065-74979084 | Msa1076450:intron | 50.0% | |
| GAATTGCCATCGCACTCTTC+CGG | - | chr7_2:74979125-74979144 | Msa1076450:intron | 50.0% | |
| CAGTGGCATCACCACCAACA+TGG | + | chr7_2:74978922-74978941 | None:intergenic | 55.0% | |
| ! | GCCACTGAGTTTGTTCCTCG+TGG | - | chr7_2:74978935-74978954 | Msa1076450:CDS | 55.0% |
| ATCAGGATAGCGTCTCCACG+AGG | + | chr7_2:74978953-74978972 | None:intergenic | 55.0% | |
| ! | TTGTGATCCTGTGCTGGCAG+AGG | - | chr7_2:74979167-74979186 | Msa1076450:intron | 55.0% |
| !! | GGACGAAAGCACCAGGTCAA+AGG | - | chr7_2:74979194-74979213 | Msa1076450:intron | 55.0% |
| ! | TCCTGTGCTGGCAGAGGTGA+CGG | - | chr7_2:74979173-74979192 | Msa1076450:intron | 60.0% |
| !! | AGGTGACGGACGAAAGCACC+AGG | - | chr7_2:74979187-74979206 | Msa1076450:intron | 60.0% |
| ! | CATGCGCCGGAAGAGTGCGA+TGG | + | chr7_2:74979134-74979153 | None:intergenic | 65.0% |
| TCCGTCACCTCTGCCAGCAC+AGG | + | chr7_2:74979177-74979196 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 74978824 | 74979281 | 74978824 | ID=Msa1076450;Name=Msa1076450 |
| chr7_2 | mRNA | 74978824 | 74979281 | 74978824 | ID=Msa1076450-mRNA-1;Parent=Msa1076450;Name=Msa1076450-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.5|1|1|2|0|70 |
| chr7_2 | exon | 74979276 | 74979281 | 74979276 | ID=Msa1076450-mRNA-1:exon:17797;Parent=Msa1076450-mRNA-1 |
| chr7_2 | exon | 74978824 | 74979030 | 74978824 | ID=Msa1076450-mRNA-1:exon:17796;Parent=Msa1076450-mRNA-1 |
| chr7_2 | CDS | 74979276 | 74979281 | 74979276 | ID=Msa1076450-mRNA-1:cds;Parent=Msa1076450-mRNA-1 |
| chr7_2 | CDS | 74978824 | 74979030 | 74978824 | ID=Msa1076450-mRNA-1:cds;Parent=Msa1076450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
