AlfalfaGEDB | Msa0142470

M. sativa cultivar zhongmu-4 / Msa0142470

KEGG: K20241
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048471 GO:0097708 GO:0098588 GO:0098791 GO:0098805
PFAM: PF00400.35

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142470	sp\|Q6LA54\|YF48_SCHPO	37.809	283	154	4	232	494	319	599	4.21e-52	196

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142470	A0A0C3Y5Y4	96.589	645	21	1	1	644	1	645	0.0	1277

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa0142470	-0.811008	8.678774e-51	-8.615850e-47
Msa0008830	Msa0142470	0.801622	8.256893e-49	-8.615850e-47
Msa0011850	Msa0142470	0.826216	3.063137e-54	-8.615850e-47
Msa0018220	Msa0142470	0.831752	1.397010e-55	-8.615850e-47
Msa0024680	Msa0142470	0.821952	3.070434e-53	-8.615850e-47
Msa0028060	Msa0142470	0.806335	8.645527e-50	-8.615850e-47
Msa0029540	Msa0142470	-0.817227	3.678300e-52	-8.615850e-47
Msa0030590	Msa0142470	0.807913	4.006336e-50	-8.615850e-47
Msa0032370	Msa0142470	0.809878	1.521750e-50	-8.615850e-47
Msa0032840	Msa0142470	-0.825330	4.971489e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142470	MtrunA17_Chr8g0384061	96.589	645	21	1	1	644	1	645	0.0	1277
Msa0142470	MtrunA17_Chr3g0129821	55.075	670	221	13	8	644	6	628	0.0	622
Msa0142470	MtrunA17_Chr1g0146771	36.023	694	315	20	46	644	50	709	4.27e-127	391
Msa0142470	MtrunA17_Chr3g0127171	37.571	708	343	20	15	642	12	700	3.22e-126	389
Msa0142470	MtrunA17_Chr8g0388841	34.553	683	345	15	47	644	49	714	1.49e-113	356
Msa0142470	MtrunA17_Chr2g0317931	35.769	520	245	8	203	644	350	858	1.64e-92	304
Msa0142470	MtrunA17_Chr7g0238321	39.481	385	182	3	195	528	385	769	3.80e-80	273
Msa0142470	MtrunA17_Chr4g0067151	27.273	286	179	9	221	498	19	283	1.29e-25	108
Msa0142470	MtrunA17_Chr1g0185501	24.911	281	171	10	223	494	21	270	2.29e-18	86.7
Msa0142470	MtrunA17_Chr3g0136971	26.554	177	107	6	226	399	458	614	1.60e-13	73.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142470	AT1G48870.2	44.111	467	205	9	193	641	162	590	1.39e-120	371
Msa0142470	AT1G48870.3	44.111	467	205	9	193	641	162	590	1.39e-120	371
Msa0142470	AT1G48870.1	44.111	467	205	9	193	641	162	590	1.39e-120	371
Msa0142470	AT5G24320.1	34.891	685	339	15	40	644	37	694	8.24e-116	362
Msa0142470	AT5G24320.2	34.789	687	337	16	40	642	37	696	2.85e-114	358
Msa0142470	AT5G53500.2	40.535	523	230	14	172	642	159	652	1.30e-112	352
Msa0142470	AT5G53500.1	40.535	523	230	14	172	642	159	652	1.30e-112	352
Msa0142470	AT5G42010.1	37.681	552	238	16	166	644	191	709	1.22e-101	325
Msa0142470	AT1G64610.2	38.140	527	236	14	169	644	160	647	1.38e-101	323
Msa0142470	AT1G64610.1	38.140	527	236	14	169	644	160	647	1.38e-101	323

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Find 162 sgRNAs with CRISPR-Local

Find 526 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGAAAACAATCAACA+TGG	0.686103	1_4:-1405343	None:intergenic
AACATCAGGAAAGGCGACAA+AGG	0.583659	1_4:+1403140	Msa0142470:CDS
AACTTCAATGACTTCAAAAG+TGG	0.509136	1_4:+1404877	Msa0142470:CDS
AAGAAGGGTTTGGTTCTGAA+AGG	0.572573	1_4:+1402691	Msa0142470:CDS
AAGATGGCGTTGTGCGCATA+TGG	0.468274	1_4:+1403345	Msa0142470:CDS
AAGGAATATATCAGAAACAT+GGG	0.572905	1_4:+1405236	Msa0142470:CDS
AAGGCGGCGTGAGTATGAAG+TGG	0.612912	1_4:+1403010	Msa0142470:CDS
AAGTGTAAATATACAAATCC+TGG	0.424976	1_4:-1405541	None:intergenic
AATCATTGTCAAATCCATGA+TGG	0.532182	1_4:+1405497	Msa0142470:three_prime_UTR
AATGAAAATTACTTTATCAG+TGG	0.560882	1_4:+1403881	Msa0142470:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCTACTACACTATTAGTT+AGG	-	chr1_4:1401818-1401837	None:intergenic	30.0%
TAATAGTGTAGTAGAGTAAC+TGG	+	chr1_4:1401821-1401840	Msa0142470:five_prime_UTR	30.0%
CCATATACTTAACATGAGAA+AGG	-	chr1_4:1402410-1402429	None:intergenic	30.0%
CCTTTCTCATGTTAAGTATA+TGG	+	chr1_4:1402407-1402426	Msa0142470:intron	30.0%
TAATTGGTGAGAGAGATATT+TGG	+	chr1_4:1402472-1402491	Msa0142470:five_prime_UTR	30.0%
TGATCTATTCTATGACTCAT+TGG	+	chr1_4:1402635-1402654	Msa0142470:CDS	30.0%
TATTAGTGTGAAGGAAAGAA+GGG	+	chr1_4:1402755-1402774	Msa0142470:CDS	30.0%
TCTTGTTGGAAGATCTTAAA+AGG	+	chr1_4:1402991-1403010	Msa0142470:CDS	30.0%
GTAAAGCTAATAGGAAAAAC+TGG	+	chr1_4:1403102-1403121	Msa0142470:CDS	30.0%
AGGTAAAGCAGAATAAAAAG+AGG	+	chr1_4:1403213-1403232	Msa0142470:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	1401658	1405750	1401658	ID=Msa0142470;Name=Msa0142470
chr1_4	mRNA	1401658	1405750	1401658	ID=Msa0142470-mRNA-1;Parent=Msa0142470;Name=Msa0142470-mRNA-1;_AED=0.15;_eAED=0.15;_QI=389\|1\|1\|1\|0.75\|0.66\|9\|415\|644
chr1_4	exon	1401658	1401842	1401658	ID=Msa0142470-mRNA-1:exon:488;Parent=Msa0142470-mRNA-1
chr1_4	exon	1402044	1402108	1402044	ID=Msa0142470-mRNA-1:exon:489;Parent=Msa0142470-mRNA-1
chr1_4	exon	1402461	1403505	1402461	ID=Msa0142470-mRNA-1:exon:490;Parent=Msa0142470-mRNA-1
chr1_4	exon	1403608	1403698	1403608	ID=Msa0142470-mRNA-1:exon:491;Parent=Msa0142470-mRNA-1
chr1_4	exon	1403855	1404012	1403855	ID=Msa0142470-mRNA-1:exon:492;Parent=Msa0142470-mRNA-1
chr1_4	exon	1404132	1404183	1404132	ID=Msa0142470-mRNA-1:exon:493;Parent=Msa0142470-mRNA-1
chr1_4	exon	1404278	1404357	1404278	ID=Msa0142470-mRNA-1:exon:494;Parent=Msa0142470-mRNA-1
chr1_4	exon	1404475	1404568	1404475	ID=Msa0142470-mRNA-1:exon:495;Parent=Msa0142470-mRNA-1
chr1_4	exon	1404782	1405750	1404782	ID=Msa0142470-mRNA-1:exon:496;Parent=Msa0142470-mRNA-1
chr1_4	five_prime_UTR	1401658	1401842	1401658	ID=Msa0142470-mRNA-1:five_prime_utr;Parent=Msa0142470-mRNA-1
chr1_4	five_prime_UTR	1402044	1402108	1402044	ID=Msa0142470-mRNA-1:five_prime_utr;Parent=Msa0142470-mRNA-1
chr1_4	five_prime_UTR	1402461	1402599	1402461	ID=Msa0142470-mRNA-1:five_prime_utr;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1402600	1403505	1402600	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1403608	1403698	1403608	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1403855	1404012	1403855	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1404132	1404183	1404132	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1404278	1404357	1404278	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1404475	1404568	1404475	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	CDS	1404782	1405335	1404782	ID=Msa0142470-mRNA-1:cds;Parent=Msa0142470-mRNA-1
chr1_4	three_prime_UTR	1405336	1405750	1405336	ID=Msa0142470-mRNA-1:three_prime_utr;Parent=Msa0142470-mRNA-1

Gene Sequence

>Msa0142470

ATGCTGAGCTGTGATGATGATGATGATGGAGATATTGATCTATTCTATGACTCATTGGATATTTTGTCTGGTGAAGATTCTGTTTTAGCCGAAGAAGGGTTTGGTTCTGAAAGGAGAGGTTGTTTTGTTTATGATGAAATTTGGGTGAATGAGCCTATTAGTGTGAAGGAAAGAAGGGAACGTTTTCTGCAGGGTATGGATTTAGCTTACACTTCTTCCAGGGTTTGTTCACAAGAGAAAACAACGAGTTTTGATGACTGGTCGGTCACATTGGGGTCGGAGAGGATCGTGGCAAGCAGTGGAGCAGTCTCAAATGCAAGCATTTCACCTTCTGGTAAAGTATCTGGGAAGTTGGTTCTCTCTGGATGGAATGCAGCTTCTGAAGCTGAAGTCTTGTTGGAAGATCTTAAAAGGCGGCGTGAGTATGAAGTGGACGCTAGTTTTCAAGAGCACAGACAGAGAGAAGGTGAGGCAAAAGAAGAATTTTGGGATTTCGATAATGGTAAAGCTAATAGGAAAAACTGGTTGAAGCGTTTTGTGAACATCAGGAAAGGCGACAAAGGAAATTTTAGATCAAAACTGAATGCAGCAACGAATAAAACTCGTAGAGTGAAGGTAAAGCAGAATAAAAAGAGGTGGATGGAGTTCAGTGAGGTATACATTGGACAAGAAATTAGAGCTCACAAAGGACTAATATGGACTATGAAATTCAGTCCCAATGGTCAGTATTTGGCTAGTGGAGGTGAAGATGGCGTTGTGCGCATATGGCGCGTTTTGTCACGTGACAAAAGCCGTCCTCCCTTTGTTTCCCTTCCAAATGATATTTTTCAAATTGAGGAATCGCCACTTCAAGAATTGTTTGGTCATTCCAGTGATATCTTGGATTTGGCCTGGTCTAATTCAGATATTCTTCTTTCGTCTTCGATGGATAAAACTGTTCGCGCTTGGAAAATCAGTTGTGATCAATGTCTAAGTGTTTTCCCTCACAAAGGCTTTGTGACATGCATTCAATTCAACCCTGTCAATGAAAATTACTTTATCAGTGGATCCATTGATGGTAAAGTTCGAATATGGGGAATACGTGAAGAACGAGTTATTGACTGGGCAGATATACGGGATGTCATAAGTGCTATAAGTTATCAACAAGATGGAAAAGGATTTGTAGTTGGTTCTCTTACAGGCACATGCCGTTTTTATGTTGCTTCAGGAAAATATTTCCAGCTTGAGGCGCAGATAAATATCCATGGAAAGAAGAAAGCATTAGGCAACAAGATTACTGGCATTCAGTTCTTCCAAAACAACTGTCAGAGAATTATGATCACATCTGAAGATTCCAAAATCTGCATATTGGATCGAACTGAGATTGTTCAGAAATACAAAGGCCTTCCATGGTCAGGAAGTCAGATGTCCGGTTCATTTACATCAAGTGGGAAACATATCATTTCAGTTGGAGAGGACTCGCGCGTGTATATTTGGAACTTCAATGACTTCAAAAGTGGTTCTTCGAAAAAAATGAAATCGGACTATTCTTGTGAGTACTTTTCCTTCAAGGGCGTAACTGTTGCAATACCTTGGTGTGGCATGAAAACAGATAGACGTGGCTGTAGAATTAACTTTTCTCACTATTCTCTGGAAGCAAAAACCAAGCTAGAGGTTACTTCTGAGGTTAGAGATACAGAACGATTTTCTCTTGGTAATTGGTTCTTCATTGATGGTACATGTCGAGGTTCTACTTCTATGACATGGCCTGAGGAGAAACTTCCAACATGGGATTTACCTCTTGTAGAAGATGAATGTGATCATCAAAATTTATGTCACGAAGATAGTAGCTATGGAAGGAATATATCAGAAACATGGGGTCTAACGATAGTAGCAGCTGGCAGTGATGGAACTATCAAGACATTCCACAATTTTGGCTTGCCTGTTAGGCTTTAG

Protein sequence

>Msa0142470

MLSCDDDDDGDIDLFYDSLDILSGEDSVLAEEGFGSERRGCFVYDEIWVNEPISVKERRERFLQGMDLAYTSSRVCSQEKTTSFDDWSVTLGSERIVASSGAVSNASISPSGKVSGKLVLSGWNAASEAEVLLEDLKRRREYEVDASFQEHRQREGEAKEEFWDFDNGKANRKNWLKRFVNIRKGDKGNFRSKLNAATNKTRRVKVKQNKKRWMEFSEVYIGQEIRAHKGLIWTMKFSPNGQYLASGGEDGVVRIWRVLSRDKSRPPFVSLPNDIFQIEESPLQELFGHSSDILDLAWSNSDILLSSSMDKTVRAWKISCDQCLSVFPHKGFVTCIQFNPVNENYFISGSIDGKVRIWGIREERVIDWADIRDVISAISYQQDGKGFVVGSLTGTCRFYVASGKYFQLEAQINIHGKKKALGNKITGIQFFQNNCQRIMITSEDSKICILDRTEIVQKYKGLPWSGSQMSGSFTSSGKHIISVGEDSRVYIWNFNDFKSGSSKKMKSDYSCEYFSFKGVTVAIPWCGMKTDRRGCRINFSHYSLEAKTKLEVTSEVRDTERFSLGNWFFIDGTCRGSTSMTWPEEKLPTWDLPLVEDECDHQNLCHEDSSYGRNISETWGLTIVAAGSDGTIKTFHNFGLPVRL*