AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1242490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1242490 MtrunA17_Chr8g0356191 93.333 195 13 0 1 195 1 195 7.59e-136 377
Msa1242490 MtrunA17_Chr8g0378461 39.665 179 102 4 13 188 15 190 2.31e-40 135
Msa1242490 MtrunA17_Chr3g0121381 51.304 115 55 1 21 135 18 131 8.90e-36 124
Msa1242490 MtrunA17_Chr7g0219391 52.885 104 48 1 9 112 10 112 3.82e-33 115
Msa1242490 MtrunA17_Chr3g0122441 32.231 121 78 3 12 128 9 129 2.80e-13 65.5
Msa1242490 MtrunA17_Chr6g0484651 38.202 89 52 1 25 113 34 119 5.24e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1242490 AT2G44300.1 50.568 176 71 3 26 186 27 201 1.82e-57 180
Msa1242490 AT2G44290.1 44.974 189 88 3 13 186 15 202 3.67e-56 177
Msa1242490 AT1G55260.1 34.239 184 117 4 4 186 46 226 4.84e-35 123
Msa1242490 AT1G55260.2 35.326 184 112 5 4 186 46 223 7.43e-35 123
Msa1242490 AT3G58550.1 37.838 148 88 3 2 147 7 152 3.78e-29 107
Msa1242490 AT1G73890.1 40.506 79 46 1 34 112 30 107 1.30e-12 63.9
Msa1242490 AT1G03103.1 28.161 174 108 5 13 182 8 168 1.11e-11 60.8
Msa1242490 AT2G48130.2 33.654 104 63 3 1 104 1 98 8.09e-11 58.9
Msa1242490 AT2G48130.6 33.654 104 63 3 1 104 1 98 8.09e-11 58.9
Msa1242490 AT2G48130.4 33.654 104 63 3 1 104 1 98 8.09e-11 58.9
Msa1242490 AT2G48130.5 33.654 104 63 3 1 104 1 98 8.09e-11 58.9
Msa1242490 AT2G48130.1 33.654 104 63 3 1 104 1 98 8.09e-11 58.9
Msa1242490 AT2G48130.3 33.654 104 63 3 1 104 1 98 8.09e-11 58.9

Find 57 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCCTATGTAGAATTTAAT+TGG 0.188962 8_2:-26808595 None:intergenic
CTACTAGCATCAATGATAAT+TGG 0.191995 8_2:+26807014 Msa1242490:CDS
AACTTGAGCCTCAGCTGATT+TGG 0.222383 8_2:-26807544 None:intergenic
TGTGCATTCTTGTTTGTCTT+TGG 0.244832 8_2:-26807057 None:intergenic
ACCAGGACATTTAGAGATAT+TGG 0.273723 8_2:-26807294 None:intergenic
GAATGCACAGCTCAATTAAC+AGG 0.295795 8_2:+26807071 Msa1242490:CDS
AATTTGACATATTTCTTCAT+AGG 0.304884 8_2:-26808642 None:intergenic
AGATCGTAATGATCCTGATT+TGG 0.324542 8_2:+26807211 Msa1242490:CDS
GATCGTAATGATCCTGATTT+GGG 0.325685 8_2:+26807212 Msa1242490:CDS
AATATGGTCGGTTTGAGTTT+TGG 0.326840 8_2:+26808325 Msa1242490:three_prime_UTR
TGAATTAGAGCTGCTGCATA+TGG 0.331829 8_2:+26807518 Msa1242490:intron
AGAAAAGAGAAGAGATTGAT+TGG 0.339081 8_2:+26808045 Msa1242490:CDS
ACTTGAGCCTCAGCTGATTT+GGG 0.345043 8_2:-26807543 None:intergenic
TGTCCCACGTGGAATTTGAT+TGG 0.393520 8_2:+26808473 Msa1242490:three_prime_UTR
TACTAGCATCAATGATAATT+GGG 0.397326 8_2:+26807015 Msa1242490:CDS
CTCAAACCGACCATATTTGC+TGG 0.398579 8_2:-26808319 None:intergenic
TATAGTAATTAATGTTACTT+TGG 0.433525 8_2:+26807238 Msa1242490:CDS
TATAAAATAAATTAATGAAT+CGG 0.447911 8_2:-26808561 None:intergenic
TCTGTGTAGTACTTCTTGCT+TGG 0.453220 8_2:-26808008 None:intergenic
ATTCCAATTAAATTCTACAT+AGG 0.459014 8_2:+26808592 Msa1242490:three_prime_UTR
TCATGTCTCCCCTATGTTGA+AGG 0.464755 8_2:+26807101 Msa1242490:CDS
GCCAATATCTCTAAATGTCC+TGG 0.474666 8_2:+26807293 Msa1242490:CDS
TTTCCTTCACCTTCAACATA+GGG 0.478002 8_2:-26807110 None:intergenic
GGACATTTAGAGATATTGGC+AGG 0.478911 8_2:-26807290 None:intergenic
AGTGCTGCACCTAGTCCATC+AGG 0.483330 8_2:+26807606 Msa1242490:CDS
GATTTGACACATTTCTTCAA+AGG 0.484214 8_2:+26808426 Msa1242490:three_prime_UTR
AGCACTTGGACTAATATTGC+TGG 0.486730 8_2:-26807589 None:intergenic
GCATCAATGATAATTGGGAT+TGG 0.502649 8_2:+26807020 Msa1242490:CDS
TTACAAAATGATAACTTCAA+AGG 0.507149 8_2:-26808226 None:intergenic
GAAAAGAGAACAATCTTCAA+GGG 0.509786 8_2:-26808182 None:intergenic
TTGAGACCATCACAACAATC+TGG 0.516643 8_2:-26807143 None:intergenic
GCAGGGGCATTACAAACTGT+TGG 0.517485 8_2:-26807272 None:intergenic
AGAAAAGAGAACAATCTTCA+AGG 0.521093 8_2:-26808183 None:intergenic
CCATCACAACAATCTGGTGC+TGG 0.553657 8_2:-26807137 None:intergenic
TTGACACATTTCTTCAAAGG+TGG 0.554115 8_2:+26808429 Msa1242490:three_prime_UTR
AAAAGTTATCACAACTCGTA+TGG 0.560372 8_2:+26808247 Msa1242490:three_prime_UTR
AGTTTCATACCTGATGGACT+AGG 0.561670 8_2:-26807615 None:intergenic
CTCCCCTATGTTGAAGGTGA+AGG 0.561730 8_2:+26807107 Msa1242490:CDS
CCAGCACCAGATTGTTGTGA+TGG 0.573645 8_2:+26807137 Msa1242490:CDS
TGACACTTGATGACTGTGTA+TGG 0.574426 8_2:-26808619 None:intergenic
ACATTTAGAGATATTGGCAG+GGG 0.578847 8_2:-26807288 None:intergenic
TTGGTTCCAGCAAATATGGT+CGG 0.579313 8_2:+26808313 Msa1242490:three_prime_UTR
GATGGACTAGGTGCAGCACT+TGG 0.582516 8_2:-26807603 None:intergenic
ATGATAATCAATATTCACAT+TGG 0.591247 8_2:-26808144 None:intergenic
GACATTTAGAGATATTGGCA+GGG 0.601482 8_2:-26807289 None:intergenic
TTCCTTCACCTTCAACATAG+GGG 0.609504 8_2:-26807109 None:intergenic
AAAAGAGAACAATCTTCAAG+GGG 0.629231 8_2:-26808181 None:intergenic
TAGTAGTAGAAAATGAGTCA+TGG 0.656551 8_2:-26806997 None:intergenic
ATTCCAATCAAATTCCACGT+GGG 0.657338 8_2:-26808476 None:intergenic
CGTAATGATCCTGATTTGGG+GGG 0.678455 8_2:+26807215 Msa1242490:CDS
CTATGTATAGTTGTCCCACG+TGG 0.680111 8_2:+26808462 Msa1242490:three_prime_UTR
TATGGATCCCAAATCAGCTG+AGG 0.682090 8_2:+26807536 Msa1242490:CDS
AATTCCAATCAAATTCCACG+TGG 0.685820 8_2:-26808477 None:intergenic
TCGTAATGATCCTGATTTGG+GGG 0.687633 8_2:+26807214 Msa1242490:CDS
ATCGTAATGATCCTGATTTG+GGG 0.695014 8_2:+26807213 Msa1242490:CDS
AATGCACAGCTCAATTAACA+GGG 0.714051 8_2:+26807072 Msa1242490:CDS
AAAGAGAACAATCTTCAAGG+GGG 0.729158 8_2:-26808180 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTCAATAATTTTTAG+TGG + chr8_2:26807907-26807926 Msa1242490:intron 10.0%
!!! ATATTTCAATTTTTTGTTTA+AGG - chr8_2:26808352-26808371 None:intergenic 10.0%
!!! AAATATTCAATAATTTTTAG+TGG + chr8_2:26807907-26807926 Msa1242490:intron 10.0%
!!! ATATTTCAATTTTTTGTTTA+AGG - chr8_2:26808352-26808371 None:intergenic 10.0%
!!! TATAGTAATTAATGTTACTT+TGG + chr8_2:26807238-26807257 Msa1242490:CDS 15.0%
!!! GTTTTTTATCAATTAAACAA+TGG + chr8_2:26807564-26807583 Msa1242490:CDS 15.0%
!!! TATGTATATATAATTTGTAG+AGG - chr8_2:26807718-26807737 None:intergenic 15.0%
!! TAAATTATTGCATATTTGTT+TGG - chr8_2:26807852-26807871 None:intergenic 15.0%
!!! TTATTTCTCAATAATTTTAG+TGG + chr8_2:26807869-26807888 Msa1242490:intron 15.0%
!!! ATTACACTAAAATAAGTTTT+TGG + chr8_2:26808294-26808313 Msa1242490:three_prime_UTR 15.0%
!!! TATAGTAATTAATGTTACTT+TGG + chr8_2:26807238-26807257 Msa1242490:CDS 15.0%
!!! GTTTTTTATCAATTAAACAA+TGG + chr8_2:26807564-26807583 Msa1242490:CDS 15.0%
!!! TATGTATATATAATTTGTAG+AGG - chr8_2:26807718-26807737 None:intergenic 15.0%
!! TAAATTATTGCATATTTGTT+TGG - chr8_2:26807852-26807871 None:intergenic 15.0%
!!! TTATTTCTCAATAATTTTAG+TGG + chr8_2:26807869-26807888 Msa1242490:intron 15.0%
!!! ATTACACTAAAATAAGTTTT+TGG + chr8_2:26808294-26808313 Msa1242490:three_prime_UTR 15.0%
!! AAATGAGTCAAATTGATTAT+GGG + chr8_2:26807360-26807379 Msa1242490:intron 20.0%
!! AACAATACATAATATATGGA+AGG - chr8_2:26807419-26807438 None:intergenic 20.0%
!! TAGGAACAATACATAATATA+TGG - chr8_2:26807423-26807442 None:intergenic 20.0%
!! GGATATAACATAACATTAAA+AGG + chr8_2:26807476-26807495 Msa1242490:intron 20.0%
!! ATATATACATAATCTCTAGA+TGG + chr8_2:26807727-26807746 Msa1242490:intron 20.0%
!!! GTTTTAGTTTGGTTTTTATT+TGG + chr8_2:26808090-26808109 Msa1242490:CDS 20.0%
!! ATGATAATCAATATTCACAT+TGG - chr8_2:26808147-26808166 None:intergenic 20.0%
!! TTACAAAATGATAACTTCAA+AGG - chr8_2:26808229-26808248 None:intergenic 20.0%
!! ATTCCAATTAAATTCTACAT+AGG + chr8_2:26808592-26808611 Msa1242490:three_prime_UTR 20.0%
!! AAATGAGTCAAATTGATTAT+GGG + chr8_2:26807360-26807379 Msa1242490:intron 20.0%
!! AACAATACATAATATATGGA+AGG - chr8_2:26807419-26807438 None:intergenic 20.0%
!! TAGGAACAATACATAATATA+TGG - chr8_2:26807423-26807442 None:intergenic 20.0%
!! GGATATAACATAACATTAAA+AGG + chr8_2:26807476-26807495 Msa1242490:intron 20.0%
!! ATATATACATAATCTCTAGA+TGG + chr8_2:26807727-26807746 Msa1242490:intron 20.0%
!!! GTTTTAGTTTGGTTTTTATT+TGG + chr8_2:26808090-26808109 Msa1242490:CDS 20.0%
!! ATGATAATCAATATTCACAT+TGG - chr8_2:26808147-26808166 None:intergenic 20.0%
!! TTACAAAATGATAACTTCAA+AGG - chr8_2:26808229-26808248 None:intergenic 20.0%
!! ATTCCAATTAAATTCTACAT+AGG + chr8_2:26808592-26808611 Msa1242490:three_prime_UTR 20.0%
! TACTAGCATCAATGATAATT+GGG + chr8_2:26807015-26807034 Msa1242490:CDS 25.0%
! TAACATAGAAAAACTTTACC+AGG - chr8_2:26807314-26807333 None:intergenic 25.0%
! GAAATGAGTCAAATTGATTA+TGG + chr8_2:26807359-26807378 Msa1242490:intron 25.0%
! TGTCCTATGTAGAATTTAAT+TGG - chr8_2:26808598-26808617 None:intergenic 25.0%
! TACTAGCATCAATGATAATT+GGG + chr8_2:26807015-26807034 Msa1242490:CDS 25.0%
! TAACATAGAAAAACTTTACC+AGG - chr8_2:26807314-26807333 None:intergenic 25.0%
! GAAATGAGTCAAATTGATTA+TGG + chr8_2:26807359-26807378 Msa1242490:intron 25.0%
! TGTCCTATGTAGAATTTAAT+TGG - chr8_2:26808598-26808617 None:intergenic 25.0%
! TAGAACATTTTTGTGTGTGT+GGG - chr8_2:26806963-26806982 None:intergenic 30.0%
TAGTAGTAGAAAATGAGTCA+TGG - chr8_2:26807000-26807019 None:intergenic 30.0%
CTACTAGCATCAATGATAAT+TGG + chr8_2:26807014-26807033 Msa1242490:CDS 30.0%
!!! GGATTTTTTTTTCGTAGTCA+GGG + chr8_2:26807381-26807400 Msa1242490:intron 30.0%
AGAAAAGAGAAGAGATTGAT+TGG + chr8_2:26808045-26808064 Msa1242490:CDS 30.0%
! GTTTAGACGTTTTGTCTATT+GGG + chr8_2:26808067-26808086 Msa1242490:CDS 30.0%
! TTTAGACGTTTTGTCTATTG+GGG + chr8_2:26808068-26808087 Msa1242490:CDS 30.0%
AAAAGAGAACAATCTTCAAG+GGG - chr8_2:26808184-26808203 None:intergenic 30.0%
GAAAAGAGAACAATCTTCAA+GGG - chr8_2:26808185-26808204 None:intergenic 30.0%
AGAAAAGAGAACAATCTTCA+AGG - chr8_2:26808186-26808205 None:intergenic 30.0%
! TGAAGATTGTTCTCTTTTCT+TGG + chr8_2:26808186-26808205 Msa1242490:three_prime_UTR 30.0%
AAAAGTTATCACAACTCGTA+TGG + chr8_2:26808247-26808266 Msa1242490:three_prime_UTR 30.0%
GATTTGACACATTTCTTCAA+AGG + chr8_2:26808426-26808445 Msa1242490:three_prime_UTR 30.0%
! TAGAACATTTTTGTGTGTGT+GGG - chr8_2:26806963-26806982 None:intergenic 30.0%
TAGTAGTAGAAAATGAGTCA+TGG - chr8_2:26807000-26807019 None:intergenic 30.0%
CTACTAGCATCAATGATAAT+TGG + chr8_2:26807014-26807033 Msa1242490:CDS 30.0%
!!! GGATTTTTTTTTCGTAGTCA+GGG + chr8_2:26807381-26807400 Msa1242490:intron 30.0%
AGAAAAGAGAAGAGATTGAT+TGG + chr8_2:26808045-26808064 Msa1242490:CDS 30.0%
! GTTTAGACGTTTTGTCTATT+GGG + chr8_2:26808067-26808086 Msa1242490:CDS 30.0%
! TTTAGACGTTTTGTCTATTG+GGG + chr8_2:26808068-26808087 Msa1242490:CDS 30.0%
AAAAGAGAACAATCTTCAAG+GGG - chr8_2:26808184-26808203 None:intergenic 30.0%
GAAAAGAGAACAATCTTCAA+GGG - chr8_2:26808185-26808204 None:intergenic 30.0%
AGAAAAGAGAACAATCTTCA+AGG - chr8_2:26808186-26808205 None:intergenic 30.0%
! TGAAGATTGTTCTCTTTTCT+TGG + chr8_2:26808186-26808205 Msa1242490:three_prime_UTR 30.0%
AAAAGTTATCACAACTCGTA+TGG + chr8_2:26808247-26808266 Msa1242490:three_prime_UTR 30.0%
GATTTGACACATTTCTTCAA+AGG + chr8_2:26808426-26808445 Msa1242490:three_prime_UTR 30.0%
! AGAACATTTTTGTGTGTGTG+GGG - chr8_2:26806962-26806981 None:intergenic 35.0%
! GTAGAACATTTTTGTGTGTG+TGG - chr8_2:26806964-26806983 None:intergenic 35.0%
GCATCAATGATAATTGGGAT+TGG + chr8_2:26807020-26807039 Msa1242490:CDS 35.0%
! TGTGCATTCTTGTTTGTCTT+TGG - chr8_2:26807060-26807079 None:intergenic 35.0%
AATGCACAGCTCAATTAACA+GGG + chr8_2:26807072-26807091 Msa1242490:CDS 35.0%
TTTCCTTCACCTTCAACATA+GGG - chr8_2:26807113-26807132 None:intergenic 35.0%
! TTTTCCTTCACCTTCAACAT+AGG - chr8_2:26807114-26807133 None:intergenic 35.0%
AGATCGTAATGATCCTGATT+TGG + chr8_2:26807211-26807230 Msa1242490:CDS 35.0%
GATCGTAATGATCCTGATTT+GGG + chr8_2:26807212-26807231 Msa1242490:CDS 35.0%
! ATCGTAATGATCCTGATTTG+GGG + chr8_2:26807213-26807232 Msa1242490:CDS 35.0%
ACATTTAGAGATATTGGCAG+GGG - chr8_2:26807291-26807310 None:intergenic 35.0%
GACATTTAGAGATATTGGCA+GGG - chr8_2:26807292-26807311 None:intergenic 35.0%
ACCAGGACATTTAGAGATAT+TGG - chr8_2:26807297-26807316 None:intergenic 35.0%
!!! GGGATTTTTTTTTCGTAGTC+AGG + chr8_2:26807380-26807399 Msa1242490:intron 35.0%
AAGCTCACGATAACTGATTA+TGG + chr8_2:26807454-26807473 Msa1242490:intron 35.0%
AGCTCACGATAACTGATTAT+GGG + chr8_2:26807455-26807474 Msa1242490:intron 35.0%
! AGAGTGTCAATCATGTTATG+TGG + chr8_2:26807966-26807985 Msa1242490:intron 35.0%
! GGTTTAGACGTTTTGTCTAT+TGG + chr8_2:26808066-26808085 Msa1242490:CDS 35.0%
!!! TGTCTATTGGGGTTTTAGTT+TGG + chr8_2:26808079-26808098 Msa1242490:CDS 35.0%
!!! TTTTTATTTGGCTTTGCTGC+TGG + chr8_2:26808102-26808121 Msa1242490:CDS 35.0%
AAAGAGAACAATCTTCAAGG+GGG - chr8_2:26808183-26808202 None:intergenic 35.0%
!!! GTTTTTGGTTCCAGCAAATA+TGG + chr8_2:26808309-26808328 Msa1242490:three_prime_UTR 35.0%
!!! AATATGGTCGGTTTGAGTTT+TGG + chr8_2:26808325-26808344 Msa1242490:three_prime_UTR 35.0%
TTGACACATTTCTTCAAAGG+TGG + chr8_2:26808429-26808448 Msa1242490:three_prime_UTR 35.0%
ATTCCAATCAAATTCCACGT+GGG - chr8_2:26808479-26808498 None:intergenic 35.0%
AATTCCAATCAAATTCCACG+TGG - chr8_2:26808480-26808499 None:intergenic 35.0%
! AGAACATTTTTGTGTGTGTG+GGG - chr8_2:26806962-26806981 None:intergenic 35.0%
! GTAGAACATTTTTGTGTGTG+TGG - chr8_2:26806964-26806983 None:intergenic 35.0%
GCATCAATGATAATTGGGAT+TGG + chr8_2:26807020-26807039 Msa1242490:CDS 35.0%
! TGTGCATTCTTGTTTGTCTT+TGG - chr8_2:26807060-26807079 None:intergenic 35.0%
AATGCACAGCTCAATTAACA+GGG + chr8_2:26807072-26807091 Msa1242490:CDS 35.0%
TTTCCTTCACCTTCAACATA+GGG - chr8_2:26807113-26807132 None:intergenic 35.0%
! TTTTCCTTCACCTTCAACAT+AGG - chr8_2:26807114-26807133 None:intergenic 35.0%
AGATCGTAATGATCCTGATT+TGG + chr8_2:26807211-26807230 Msa1242490:CDS 35.0%
GATCGTAATGATCCTGATTT+GGG + chr8_2:26807212-26807231 Msa1242490:CDS 35.0%
! ATCGTAATGATCCTGATTTG+GGG + chr8_2:26807213-26807232 Msa1242490:CDS 35.0%
ACATTTAGAGATATTGGCAG+GGG - chr8_2:26807291-26807310 None:intergenic 35.0%
GACATTTAGAGATATTGGCA+GGG - chr8_2:26807292-26807311 None:intergenic 35.0%
ACCAGGACATTTAGAGATAT+TGG - chr8_2:26807297-26807316 None:intergenic 35.0%
!!! GGGATTTTTTTTTCGTAGTC+AGG + chr8_2:26807380-26807399 Msa1242490:intron 35.0%
AAGCTCACGATAACTGATTA+TGG + chr8_2:26807454-26807473 Msa1242490:intron 35.0%
AGCTCACGATAACTGATTAT+GGG + chr8_2:26807455-26807474 Msa1242490:intron 35.0%
! AGAGTGTCAATCATGTTATG+TGG + chr8_2:26807966-26807985 Msa1242490:intron 35.0%
! GGTTTAGACGTTTTGTCTAT+TGG + chr8_2:26808066-26808085 Msa1242490:CDS 35.0%
!!! TGTCTATTGGGGTTTTAGTT+TGG + chr8_2:26808079-26808098 Msa1242490:CDS 35.0%
!!! TTTTTATTTGGCTTTGCTGC+TGG + chr8_2:26808102-26808121 Msa1242490:CDS 35.0%
AAAGAGAACAATCTTCAAGG+GGG - chr8_2:26808183-26808202 None:intergenic 35.0%
!!! GTTTTTGGTTCCAGCAAATA+TGG + chr8_2:26808309-26808328 Msa1242490:three_prime_UTR 35.0%
!!! AATATGGTCGGTTTGAGTTT+TGG + chr8_2:26808325-26808344 Msa1242490:three_prime_UTR 35.0%
TTGACACATTTCTTCAAAGG+TGG + chr8_2:26808429-26808448 Msa1242490:three_prime_UTR 35.0%
ATTCCAATCAAATTCCACGT+GGG - chr8_2:26808479-26808498 None:intergenic 35.0%
AATTCCAATCAAATTCCACG+TGG - chr8_2:26808480-26808499 None:intergenic 35.0%
GTGGGGATATGTGAAAAAGT+GGG - chr8_2:26806945-26806964 None:intergenic 40.0%
TGTGGGGATATGTGAAAAAG+TGG - chr8_2:26806946-26806965 None:intergenic 40.0%
! GAGTCATGGCTTTTGTGTTT+TGG - chr8_2:26806986-26807005 None:intergenic 40.0%
GAATGCACAGCTCAATTAAC+AGG + chr8_2:26807071-26807090 Msa1242490:CDS 40.0%
TTCCTTCACCTTCAACATAG+GGG - chr8_2:26807112-26807131 None:intergenic 40.0%
TTGAGACCATCACAACAATC+TGG - chr8_2:26807146-26807165 None:intergenic 40.0%
!! TCGTAATGATCCTGATTTGG+GGG + chr8_2:26807214-26807233 Msa1242490:CDS 40.0%
ATTACTATACCCCCCAAATC+AGG - chr8_2:26807227-26807246 None:intergenic 40.0%
GGACATTTAGAGATATTGGC+AGG - chr8_2:26807293-26807312 None:intergenic 40.0%
GCCAATATCTCTAAATGTCC+TGG + chr8_2:26807293-26807312 Msa1242490:CDS 40.0%
TATCGTGAGCTTAGATCAGT+TGG - chr8_2:26807446-26807465 None:intergenic 40.0%
TGAATTAGAGCTGCTGCATA+TGG + chr8_2:26807518-26807537 Msa1242490:intron 40.0%
AGCACTTGGACTAATATTGC+TGG - chr8_2:26807592-26807611 None:intergenic 40.0%
!! AGTTTCATACCTGATGGACT+AGG - chr8_2:26807618-26807637 None:intergenic 40.0%
AAGCGAAGTTTCATACCTGA+TGG - chr8_2:26807624-26807643 None:intergenic 40.0%
TCATGTTATGTGGCTGTAGA+TGG + chr8_2:26807976-26807995 Msa1242490:intron 40.0%
TCTGTGTAGTACTTCTTGCT+TGG - chr8_2:26808011-26808030 None:intergenic 40.0%
TTGGTTCCAGCAAATATGGT+CGG + chr8_2:26808313-26808332 Msa1242490:three_prime_UTR 40.0%
! TGACACTTGATGACTGTGTA+TGG - chr8_2:26808622-26808641 None:intergenic 40.0%
GTGGGGATATGTGAAAAAGT+GGG - chr8_2:26806945-26806964 None:intergenic 40.0%
TGTGGGGATATGTGAAAAAG+TGG - chr8_2:26806946-26806965 None:intergenic 40.0%
! GAGTCATGGCTTTTGTGTTT+TGG - chr8_2:26806986-26807005 None:intergenic 40.0%
GAATGCACAGCTCAATTAAC+AGG + chr8_2:26807071-26807090 Msa1242490:CDS 40.0%
TTCCTTCACCTTCAACATAG+GGG - chr8_2:26807112-26807131 None:intergenic 40.0%
TTGAGACCATCACAACAATC+TGG - chr8_2:26807146-26807165 None:intergenic 40.0%
!! TCGTAATGATCCTGATTTGG+GGG + chr8_2:26807214-26807233 Msa1242490:CDS 40.0%
ATTACTATACCCCCCAAATC+AGG - chr8_2:26807227-26807246 None:intergenic 40.0%
GGACATTTAGAGATATTGGC+AGG - chr8_2:26807293-26807312 None:intergenic 40.0%
GCCAATATCTCTAAATGTCC+TGG + chr8_2:26807293-26807312 Msa1242490:CDS 40.0%
TATCGTGAGCTTAGATCAGT+TGG - chr8_2:26807446-26807465 None:intergenic 40.0%
TGAATTAGAGCTGCTGCATA+TGG + chr8_2:26807518-26807537 Msa1242490:intron 40.0%
AGCACTTGGACTAATATTGC+TGG - chr8_2:26807592-26807611 None:intergenic 40.0%
!! AGTTTCATACCTGATGGACT+AGG - chr8_2:26807618-26807637 None:intergenic 40.0%
AAGCGAAGTTTCATACCTGA+TGG - chr8_2:26807624-26807643 None:intergenic 40.0%
TCATGTTATGTGGCTGTAGA+TGG + chr8_2:26807976-26807995 Msa1242490:intron 40.0%
TCTGTGTAGTACTTCTTGCT+TGG - chr8_2:26808011-26808030 None:intergenic 40.0%
TTGGTTCCAGCAAATATGGT+CGG + chr8_2:26808313-26808332 Msa1242490:three_prime_UTR 40.0%
! TGACACTTGATGACTGTGTA+TGG - chr8_2:26808622-26808641 None:intergenic 40.0%
TCATGTCTCCCCTATGTTGA+AGG + chr8_2:26807101-26807120 Msa1242490:CDS 45.0%
!! CGTAATGATCCTGATTTGGG+GGG + chr8_2:26807215-26807234 Msa1242490:CDS 45.0%
TCGTAGTCAGGGTTTGAACT+CGG + chr8_2:26807392-26807411 Msa1242490:intron 45.0%
GTGAGCTTAGATCAGTTGGT+AGG - chr8_2:26807442-26807461 None:intergenic 45.0%
TATGGATCCCAAATCAGCTG+AGG + chr8_2:26807536-26807555 Msa1242490:CDS 45.0%
! ACTTGAGCCTCAGCTGATTT+GGG - chr8_2:26807546-26807565 None:intergenic 45.0%
AACTTGAGCCTCAGCTGATT+TGG - chr8_2:26807547-26807566 None:intergenic 45.0%
!! TCTTCAAGGGGGAAAGTGTT+TGG - chr8_2:26808172-26808191 None:intergenic 45.0%
CTCAAACCGACCATATTTGC+TGG - chr8_2:26808322-26808341 None:intergenic 45.0%
CTATGTATAGTTGTCCCACG+TGG + chr8_2:26808462-26808481 Msa1242490:three_prime_UTR 45.0%
TGTCCCACGTGGAATTTGAT+TGG + chr8_2:26808473-26808492 Msa1242490:three_prime_UTR 45.0%
TCATGTCTCCCCTATGTTGA+AGG + chr8_2:26807101-26807120 Msa1242490:CDS 45.0%
!! CGTAATGATCCTGATTTGGG+GGG + chr8_2:26807215-26807234 Msa1242490:CDS 45.0%
TCGTAGTCAGGGTTTGAACT+CGG + chr8_2:26807392-26807411 Msa1242490:intron 45.0%
GTGAGCTTAGATCAGTTGGT+AGG - chr8_2:26807442-26807461 None:intergenic 45.0%
TATGGATCCCAAATCAGCTG+AGG + chr8_2:26807536-26807555 Msa1242490:CDS 45.0%
! ACTTGAGCCTCAGCTGATTT+GGG - chr8_2:26807546-26807565 None:intergenic 45.0%
AACTTGAGCCTCAGCTGATT+TGG - chr8_2:26807547-26807566 None:intergenic 45.0%
!! TCTTCAAGGGGGAAAGTGTT+TGG - chr8_2:26808172-26808191 None:intergenic 45.0%
CTCAAACCGACCATATTTGC+TGG - chr8_2:26808322-26808341 None:intergenic 45.0%
CTATGTATAGTTGTCCCACG+TGG + chr8_2:26808462-26808481 Msa1242490:three_prime_UTR 45.0%
TGTCCCACGTGGAATTTGAT+TGG + chr8_2:26808473-26808492 Msa1242490:three_prime_UTR 45.0%
!!! ATATAAAACAATTTTATAAT+AGG - chr8_2:26807757-26807776 None:intergenic 5.0%
!! TATAAAATAAATTAATGAAT+CGG - chr8_2:26808564-26808583 None:intergenic 5.0%
!!! ATATAAAACAATTTTATAAT+AGG - chr8_2:26807757-26807776 None:intergenic 5.0%
!! TATAAAATAAATTAATGAAT+CGG - chr8_2:26808564-26808583 None:intergenic 5.0%
CTCCCCTATGTTGAAGGTGA+AGG + chr8_2:26807107-26807126 Msa1242490:CDS 50.0%
CCATCACAACAATCTGGTGC+TGG - chr8_2:26807140-26807159 None:intergenic 50.0%
CCAGCACCAGATTGTTGTGA+TGG + chr8_2:26807137-26807156 Msa1242490:CDS 50.0%
!! GCAGGGGCATTACAAACTGT+TGG - chr8_2:26807275-26807294 None:intergenic 50.0%
CTCCCCTATGTTGAAGGTGA+AGG + chr8_2:26807107-26807126 Msa1242490:CDS 50.0%
CCATCACAACAATCTGGTGC+TGG - chr8_2:26807140-26807159 None:intergenic 50.0%
CCAGCACCAGATTGTTGTGA+TGG + chr8_2:26807137-26807156 Msa1242490:CDS 50.0%
!! GCAGGGGCATTACAAACTGT+TGG - chr8_2:26807275-26807294 None:intergenic 50.0%
!! GATGGACTAGGTGCAGCACT+TGG - chr8_2:26807606-26807625 None:intergenic 55.0%
AGTGCTGCACCTAGTCCATC+AGG + chr8_2:26807606-26807625 Msa1242490:CDS 55.0%
!! GATGGACTAGGTGCAGCACT+TGG - chr8_2:26807606-26807625 None:intergenic 55.0%
AGTGCTGCACCTAGTCCATC+AGG + chr8_2:26807606-26807625 Msa1242490:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 26806925 26808649 26806925 ID=Msa1242490;Name=Msa1242490
chr8_2 mRNA 26806925 26808649 26806925 ID=Msa1242490-mRNA-1;Parent=Msa1242490;Name=Msa1242490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=50|1|1|1|1|1|3|509|195
chr8_2 exon 26806925 26807314 26806925 ID=Msa1242490-mRNA-1:exon:9413;Parent=Msa1242490-mRNA-1
chr8_2 exon 26807526 26807627 26807526 ID=Msa1242490-mRNA-1:exon:9414;Parent=Msa1242490-mRNA-1
chr8_2 exon 26807995 26808649 26807995 ID=Msa1242490-mRNA-1:exon:9415;Parent=Msa1242490-mRNA-1
chr8_2 five_prime_UTR 26806925 26806974 26806925 ID=Msa1242490-mRNA-1:five_prime_utr;Parent=Msa1242490-mRNA-1
chr8_2 CDS 26806975 26807314 26806975 ID=Msa1242490-mRNA-1:cds;Parent=Msa1242490-mRNA-1
chr8_2 CDS 26807526 26807627 26807526 ID=Msa1242490-mRNA-1:cds;Parent=Msa1242490-mRNA-1
chr8_2 CDS 26807995 26808140 26807995 ID=Msa1242490-mRNA-1:cds;Parent=Msa1242490-mRNA-1
chr8_2 three_prime_UTR 26808141 26808649 26808141 ID=Msa1242490-mRNA-1:three_prime_utr;Parent=Msa1242490-mRNA-1
Gene Sequence

>Msa1242490

ATGTTCTACCAAAACACAAAAGCCATGACTCATTTTCTACTACTAGCATCAATGATAATTGGGATTGGAATGTCAGATTCATCCAAAGACAAACAAGAATGCACAGCTCAATTAACAGGGCTAGCATCATGTCTCCCCTATGTTGAAGGTGAAGGAAAAACTCCAGCACCAGATTGTTGTGATGGTCTCAAGACACTTCTCAAGACAAACAAGAAGTGTTTGTGTGTAATTATCAAAGATCGTAATGATCCTGATTTGGGGGGTATAGTAATTAATGTTACTTTGGCATTGAATCTTCCAACAGTTTGTAATGCCCCTGCCAATATCTCTAAATGTCCTGAGCTGCTGCATATGGATCCCAAATCAGCTGAGGCTCAAGTTTTTTATCAATTAAACAATGGCTCCAGCAATATTAGTCCAAGTGCTGCACCTAGTCCATCAGATGGAGCAAGCAACCAAGCAAGAAGTACTACACAGAAGAATGATGCATTCAGAAAAGAGAAGAGATTGATTGGTTTAGACGTTTTGTCTATTGGGGTTTTAGTTTGGTTTTTATTTGGCTTTGCTGCTGGAAACTTTTTCATTTGA
Protein sequence

>Msa1242490

MFYQNTKAMTHFLLLASMIIGIGMSDSSKDKQECTAQLTGLASCLPYVEGEGKTPAPDCCDGLKTLLKTNKKCLCVIIKDRNDPDLGGIVINVTLALNLPTVCNAPANISKCPELLHMDPKSAEAQVFYQLNNGSSNISPSAAPSPSDGASNQARSTTQKNDAFRKEKRLIGLDVLSIGVLVWFLFGFAAGNFFI*