AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1459840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1459840 MtrunA17_Chr4g0069751 96.450 169 6 0 1 169 1 169 1.60e-114 322
Msa1459840 MtrunA17_Chr4g0069761 83.784 111 9 1 59 169 1 102 7.01e-63 189
Msa1459840 MtrunA17_Chr5g0397351 39.024 82 44 1 58 139 60 135 3.79e-16 70.9
Msa1459840 MtrunA17_Chr7g0248101 38.667 75 40 1 58 132 69 137 1.66e-15 70.5
Msa1459840 MtrunA17_Chr8g0387291 37.931 87 44 2 46 132 57 133 3.05e-15 69.3
Msa1459840 MtrunA17_Chr8g0348551 40.260 77 34 3 58 132 73 139 7.16e-13 63.2
Msa1459840 MtrunA17_Chr8g0336881 43.373 83 39 2 55 136 80 155 1.11e-12 64.7
Msa1459840 MtrunA17_Chr2g0301871 44.872 78 35 2 60 136 63 133 1.13e-12 64.3
Msa1459840 MtrunA17_Chr3g0120681 41.667 72 36 1 61 132 81 146 1.40e-12 62.0
Msa1459840 MtrunA17_Chr4g0073141 43.284 67 27 2 79 145 144 199 1.38e-11 61.6
Msa1459840 MtrunA17_Chr2g0295571 44.444 72 31 3 60 131 102 164 8.14e-11 58.9
Msa1459840 MtrunA17_Chr3g0132491 50.000 54 20 2 79 132 136 182 8.92e-11 59.3
Msa1459840 MtrunA17_Chr5g0426881 46.296 54 22 1 79 132 143 189 9.84e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1459840 AT3G43430.1 41.808 177 84 6 1 168 1 167 3.52e-41 136
Msa1459840 AT5G20885.1 42.935 184 77 8 1 166 1 174 5.28e-40 133
Msa1459840 AT3G61460.1 29.252 147 83 5 1 132 1 141 1.87e-16 73.2
Msa1459840 AT1G63840.1 31.757 148 74 7 1 132 1 137 1.97e-15 70.1
Msa1459840 AT4G00305.1 41.026 78 39 2 58 135 51 121 3.19e-15 68.6
Msa1459840 AT5G41400.1 39.241 79 40 3 58 134 83 155 9.61e-15 68.6
Msa1459840 AT3G60220.1 42.105 95 44 3 43 136 83 167 6.54e-14 68.6
Msa1459840 AT2G01150.1 53.704 54 18 2 79 132 74 120 2.04e-12 62.0
Msa1459840 AT2G01150.3 53.704 54 18 2 79 132 100 146 2.27e-12 62.4
Msa1459840 AT2G01150.2 53.704 54 18 2 79 132 123 169 3.93e-12 62.0

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACATGTTTCTTCTGTTTC+TGG 0.111435 tig0040489:+7838 Msa1459840:intergenic
GGCTTGACTTATTGTTAAAT+TGG 0.164893 tig0040489:+8026 Msa1459840:intergenic
TATTGTTGTTGTTCTTCTTC+TGG 0.235426 tig0040489:+7919 Msa1459840:intergenic
GAGAGACTTCTTTATCTCTT+TGG 0.276051 tig0040489:-7589 Msa1459840:CDS
ATGCTACCTTTGACAAGTTT+CGG 0.327811 tig0040489:-7880 Msa1459840:CDS
TAATCATTGCAGTTCTTAGA+TGG 0.340842 tig0040489:-7987 Msa1459840:CDS
TTCTTCTCCTCAACCTAGTT+GGG 0.344519 tig0040489:-7617 Msa1459840:CDS
GATTAGAGCAGCTTCATATA+TGG 0.344672 tig0040489:+8005 Msa1459840:intergenic
TTGTTTGAACAAGCTGAAAA+TGG 0.363273 tig0040489:-7812 Msa1459840:CDS
TTTCTTCTCCTCAACCTAGT+TGG 0.373720 tig0040489:-7618 Msa1459840:CDS
CTCTACAAAGTGGACAAGTT+TGG 0.375846 tig0040489:+7692 Msa1459840:intergenic
GACAAGTTTCGGAGAAATCA+AGG 0.419612 tig0040489:-7869 Msa1459840:CDS
ACTTATTGTTAAATTGGAGA+TGG 0.483621 tig0040489:+8032 Msa1459840:intergenic
ATAAGGAATGCATAGATAAG+TGG 0.495925 tig0040489:-7747 Msa1459840:CDS
CTGTGATCATGTGTTTCATA+AGG 0.517073 tig0040489:-7764 Msa1459840:CDS
AGTCTCTCAACAGCCCAACT+AGG 0.548383 tig0040489:+7604 Msa1459840:intergenic
TCAACAGCCCAACTAGGTTG+AGG 0.559804 tig0040489:+7610 Msa1459840:intergenic
TCATCTGAGATTTCAAGCTG+TGG 0.568106 tig0040489:-7643 Msa1459840:CDS
TAACTAATTATTAATTAACA+AGG 0.588753 tig0040489:+7553 Msa1459840:intergenic
ATTTCTCCGAAACTTGTCAA+AGG 0.593001 tig0040489:+7874 Msa1459840:intergenic
AGTGAATAAGAATTAATCAA+AGG 0.595804 tig0040489:+7667 Msa1459840:intergenic
ATCAAAGGAGCTCTACAAAG+TGG 0.596806 tig0040489:+7682 Msa1459840:intergenic
ATAGATAAGTGGTTAGAACA+TGG 0.680848 tig0040489:-7736 Msa1459840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTGAATAAGAATTAATCAA+AGG + tig0040489:7957-7976 Msa1459840:intergenic 20.0%
! ACTTATTGTTAAATTGGAGA+TGG + tig0040489:7592-7611 Msa0135390:intergenic 25.0%
GGCTTGACTTATTGTTAAAT+TGG + tig0040489:7598-7617 Msa0135390:intergenic 30.0%
TAATCATTGCAGTTCTTAGA+TGG - tig0040489:7634-7653 Msa1459840:CDS 30.0%
! TATTGTTGTTGTTCTTCTTC+TGG + tig0040489:7705-7724 Msa1459840:intergenic 30.0%
TTGTTTGAACAAGCTGAAAA+TGG - tig0040489:7809-7828 Msa1459840:CDS 30.0%
ATAAGGAATGCATAGATAAG+TGG - tig0040489:7874-7893 Msa1459840:CDS 30.0%
ATAGATAAGTGGTTAGAACA+TGG - tig0040489:7885-7904 Msa1459840:CDS 30.0%
GATTAGAGCAGCTTCATATA+TGG + tig0040489:7619-7638 Msa0135390:intergenic 35.0%
ATGCTACCTTTGACAAGTTT+CGG - tig0040489:7741-7760 Msa1459840:CDS 35.0%
ATTTCTCCGAAACTTGTCAA+AGG + tig0040489:7750-7769 Msa1459840:intergenic 35.0%
CTGTGATCATGTGTTTCATA+AGG - tig0040489:7857-7876 Msa1459840:CDS 35.0%
GAGAGACTTCTTTATCTCTT+TGG - tig0040489:8032-8051 Msa1459840:CDS 35.0%
GACAAGTTTCGGAGAAATCA+AGG - tig0040489:7752-7771 Msa1459840:CDS 40.0%
GCACATGTTTCTTCTGTTTC+TGG + tig0040489:7786-7805 Msa1459840:intergenic 40.0%
CTCTACAAAGTGGACAAGTT+TGG + tig0040489:7932-7951 Msa1459840:intergenic 40.0%
ATCAAAGGAGCTCTACAAAG+TGG + tig0040489:7942-7961 Msa1459840:intergenic 40.0%
! TCATCTGAGATTTCAAGCTG+TGG - tig0040489:7978-7997 Msa1459840:CDS 40.0%
TTTCTTCTCCTCAACCTAGT+TGG - tig0040489:8003-8022 Msa1459840:CDS 40.0%
TTCTTCTCCTCAACCTAGTT+GGG - tig0040489:8004-8023 Msa1459840:CDS 40.0%
TCAACAGCCCAACTAGGTTG+AGG + tig0040489:8014-8033 Msa1459840:intergenic 50.0%
AGTCTCTCAACAGCCCAACT+AGG + tig0040489:8020-8039 Msa1459840:intergenic 50.0%
Chromosome Type Strat End Strand Name
tig0040489 gene 7567 8076 7567 ID=Msa1459840;Name=Msa1459840
tig0040489 mRNA 7567 8076 7567 ID=Msa1459840-mRNA-1;Parent=Msa1459840;Name=Msa1459840-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|169
tig0040489 exon 7567 8076 7567 ID=Msa1459840-mRNA-1:exon:22445;Parent=Msa1459840-mRNA-1
tig0040489 CDS 7567 8076 7567 ID=Msa1459840-mRNA-1:cds;Parent=Msa1459840-mRNA-1
Gene Sequence

>Msa1459840

ATGGGTTTCTACACTCAACAATCCATCTCCAATTTAACAATAAGTCAAGCCATATATGAAGCTGCTCTAATCATTGCAGTTCTTAGATGGATCATATGCTTAGTTTTCAAAATTATCAAACACACAAAAGCAAAACCAGAAGAAGAACAACAACAATACTCTTCTTGTTGTCAAATGCTACCTTTGACAAGTTTCGGAGAAATCAAGGAAAGACATCCAGAAACAGAAGAAACATGTGCTGTTTGTTTGAACAAGCTGAAAATGGAAGATGAAGTTAGAGAATTGATGAACTGTGATCATGTGTTTCATAAGGAATGCATAGATAAGTGGTTAGAACATGGTCATGAAAATGAAAATCATAACCAAACTTGTCCACTTTGTAGAGCTCCTTTGATTAATTCTTATTCACTTTCATCTGAGATTTCAAGCTGTGGATTTTCTTCTCCTCAACCTAGTTGGGCTGTTGAGAGACTTCTTTATCTCTTTGGTGATGATCTCTTGCCTTGTTAA

Protein sequence

>Msa1459840

MGFYTQQSISNLTISQAIYEAALIIAVLRWIICLVFKIIKHTKAKPEEEQQQYSSCCQMLPLTSFGEIKERHPETEETCAVCLNKLKMEDEVRELMNCDHVFHKECIDKWLEHGHENENHNQTCPLCRAPLINSYSLSSEISSCGFSSPQPSWAVERLLYLFGDDLLPC*