AlfalfaGEDB | Msa0044050

M. sativa cultivar zhongmu-4 / Msa0044050

KEGG: K02639
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF00111.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0044050	sp\|P27788\|FER3_MAIZE	65.132	152	50	3	11	159	1	152	4.59e-61	188

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0044050	0.814838	1.256146e-51	-8.615850e-47
Msa0043960	Msa0044050	0.988304	1.692690e-173	-8.615850e-47
Msa0044050	Msa0047470	-0.816482	5.405946e-52	-8.615850e-47
Msa0044050	Msa0057740	-0.810225	1.281297e-50	-8.615850e-47
Msa0044050	Msa0090620	0.982939	2.111631e-156	-8.615850e-47
Msa0044050	Msa0103060	-0.821225	4.521845e-53	-8.615850e-47
Msa0044050	Msa0129270	0.808705	2.716239e-50	-8.615850e-47
Msa0044050	Msa0183800	0.988871	9.439096e-176	-8.615850e-47
Msa0044050	Msa0185330	0.800798	1.217072e-48	-8.615850e-47
Msa0044050	Msa0185390	-0.809959	1.462128e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0044050	MtrunA17_Chr1g0210631	94.969	159	8	0	1	159	60	218	6.83e-110	311
Msa0044050	MtrunA17_Chr1g0210661	90.196	153	15	0	6	158	1	153	7.88e-101	286
Msa0044050	MtrunA17_Chr2g0321501	72.727	132	35	1	29	159	24	155	2.19e-67	201
Msa0044050	MtrunA17_Chr4g0026331	65.385	156	48	3	6	159	4	155	7.21e-66	197
Msa0044050	MtrunA17_Chr2g0277481	55.102	147	60	3	17	158	3	148	5.66e-50	157
Msa0044050	MtrunA17_Chr8g0380641	55.556	144	61	3	17	158	4	146	7.22e-50	157
Msa0044050	MtrunA17_Chr5g0394481	31.757	148	96	3	11	158	1	143	1.82e-19	79.7
Msa0044050	MtrunA17_Chr4g0054691	39.535	86	52	0	74	159	74	159	1.06e-18	78.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0044050	AT2G27510.1	60.000	155	56	3	11	159	1	155	2.73e-60	184
Msa0044050	AT1G60950.1	56.209	153	56	5	11	158	1	147	6.51e-50	157
Msa0044050	AT1G10960.1	58.915	129	47	3	35	158	20	147	6.27e-47	150
Msa0044050	AT5G10000.1	52.381	126	57	2	36	158	22	147	8.15e-40	132
Msa0044050	AT4G14890.1	44.554	101	53	3	58	158	47	144	7.59e-20	81.3
Msa0044050	AT1G32550.1	39.535	86	52	0	74	159	69	154	3.95e-18	77.4
Msa0044050	AT1G32550.2	37.255	102	45	3	74	159	69	167	8.37e-13	63.5

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Find 40 sgRNAs with CRISPR-Local

Find 299 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAAGGAAACTTGTTAT+AGG	0.331567	1_1:-75518897	Msa0044050:three_prime_UTR
AAGAATGTGTCCAAAACATT+TGG	0.421132	1_1:-75519362	Msa0044050:CDS
AATTCGTTCTCTGTTCCATC+TGG	0.449513	1_1:+75519278	None:intergenic
ACACATAGTCAAAACATGCA+CGG	0.652038	1_1:+75518985	None:intergenic
AGAGCACAAGATGTCCGAGA+GGG	0.687025	1_1:+75519416	None:intergenic
AGCTGTTGAAAGTCTCACGG+TGG	0.759008	1_1:+75519457	None:intergenic
ATCATTTAGAGTATCTGCAA+TGG	0.596293	1_1:-75519327	Msa0044050:CDS
CACACCATATCATTAACAAC+TGG	0.471108	1_1:+75518797	None:intergenic
CAGAATGTAAGAGTCATCAG+GGG	0.754480	1_1:+75519250	None:intergenic
CAGAGCACAAGATGTCCGAG+AGG	0.730685	1_1:+75519415	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATAAAAGGGAAATGGAA+AGG	+	chr1_1:75518890-75518909	None:intergenic	30.0%
ATCTCACAGTGAAGATAAAA+GGG	+	chr1_1:75518901-75518920	None:intergenic	30.0%
AATCTCACAGTGAAGATAAA+AGG	+	chr1_1:75518902-75518921	None:intergenic	30.0%
TATTTCATTCTCTCATCCTA+TGG	-	chr1_1:75519200-75519219	Msa0044050:CDS	30.0%
TTTATCCACTTCAGGAATTA+GGG	+	chr1_1:75519450-75519469	None:intergenic	30.0%
TAATTCCTGAAGTGGATAAA+AGG	-	chr1_1:75519450-75519469	Msa0044050:CDS	30.0%
AATTCCTGAAGTGGATAAAA+GGG	-	chr1_1:75519451-75519470	Msa0044050:CDS	30.0%
TAAAAACCAAATACGCATAG+AGG	+	chr1_1:75520152-75520171	None:intergenic	30.0%
TTTCTTGTAAGAACTTTCAG+GGG	-	chr1_1:75520311-75520330	Msa0044050:intron	30.0%
AAGAATGTGTCCAAAACATT+TGG	-	chr1_1:75520564-75520583	Msa0044050:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	75518783	75521165	75518783	ID=Msa0044050;Name=Msa0044050
chr1_1	mRNA	75518783	75521165	75518783	ID=Msa0044050-mRNA-1;Parent=Msa0044050;Name=Msa0044050-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0\|0.5\|0.5\|1\|1\|2\|251\|159
chr1_1	exon	75518783	75519506	75518783	ID=Msa0044050-mRNA-1:exon:25497;Parent=Msa0044050-mRNA-1
chr1_1	exon	75521159	75521165	75521159	ID=Msa0044050-mRNA-1:exon:25496;Parent=Msa0044050-mRNA-1
chr1_1	CDS	75521159	75521165	75521159	ID=Msa0044050-mRNA-1:cds;Parent=Msa0044050-mRNA-1
chr1_1	CDS	75519034	75519506	75519034	ID=Msa0044050-mRNA-1:cds;Parent=Msa0044050-mRNA-1
chr1_1	three_prime_UTR	75518783	75519033	75518783	ID=Msa0044050-mRNA-1:three_prime_utr;Parent=Msa0044050-mRNA-1

Gene Sequence

>Msa0044050

ATGGAAAGTATAAAAATGTCAGCCGTGAATATGTCCACCGTGAGACTTTCAACAGCTTCATTGTACGGAACAGTGCCCTCTCGGACATCTTGTGCTCTGATGAAGAGTCCATCAAGTTTGAGATCTGTGAAGAATGTGTCCAAAACATTTGGACTAAAATCTTCATCATTTAGAGTATCTGCAATGGCAGTATACAAAGTGAAACTGATTGGACCAGATGGAACAGAGAACGAATTTGATGCCCCTGATGACTCTTACATTCTGGATTCAGCTGAAGATGCTGGAGTGGAGCTACCTTATTCATGCAGAGCGGGTGCATGTTCTACTTGTGCTGGCCAAGTGGTTACGGGTTCTGTCGATCAGTCTGATCAATCTTTCCTCGATGAGCAGCAAATCGAGAAGGGTTATCTTCTCACTTGTGTCTCATACCCAAAATCAGATACTGTGATTTACACACACAAGGAGGAAGAACTCTACTAA

Protein sequence

>Msa0044050

MESIKMSAVNMSTVRLSTASLYGTVPSRTSCALMKSPSSLRSVKNVSKTFGLKSSSFRVSAMAVYKVKLIGPDGTENEFDAPDDSYILDSAEDAGVELPYSCRAGACSTCAGQVVTGSVDQSDQSFLDEQQIEKGYLLTCVSYPKSDTVIYTHKEEELY*