AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0044050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0044050 MtrunA17_Chr1g0210631 94.969 159 8 0 1 159 60 218 6.83e-110 311
Msa0044050 MtrunA17_Chr1g0210661 90.196 153 15 0 6 158 1 153 7.88e-101 286
Msa0044050 MtrunA17_Chr2g0321501 72.727 132 35 1 29 159 24 155 2.19e-67 201
Msa0044050 MtrunA17_Chr4g0026331 65.385 156 48 3 6 159 4 155 7.21e-66 197
Msa0044050 MtrunA17_Chr2g0277481 55.102 147 60 3 17 158 3 148 5.66e-50 157
Msa0044050 MtrunA17_Chr8g0380641 55.556 144 61 3 17 158 4 146 7.22e-50 157
Msa0044050 MtrunA17_Chr5g0394481 31.757 148 96 3 11 158 1 143 1.82e-19 79.7
Msa0044050 MtrunA17_Chr4g0054691 39.535 86 52 0 74 159 74 159 1.06e-18 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0044050 AT2G27510.1 60.000 155 56 3 11 159 1 155 2.73e-60 184
Msa0044050 AT1G60950.1 56.209 153 56 5 11 158 1 147 6.51e-50 157
Msa0044050 AT1G10960.1 58.915 129 47 3 35 158 20 147 6.27e-47 150
Msa0044050 AT5G10000.1 52.381 126 57 2 36 158 22 147 8.15e-40 132
Msa0044050 AT4G14890.1 44.554 101 53 3 58 158 47 144 7.59e-20 81.3
Msa0044050 AT1G32550.1 39.535 86 52 0 74 159 69 154 3.95e-18 77.4
Msa0044050 AT1G32550.2 37.255 102 45 3 74 159 69 167 8.37e-13 63.5

Find 40 sgRNAs with CRISPR-Local

Find 299 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATCACAGTATCTGATTT+TGG 0.126493 1_1:+75519062 None:intergenic
TTCACAGATCTCAAACTTGA+TGG 0.324898 1_1:+75519383 None:intergenic
TTGTGCTGGCCAAGTGGTTA+CGG 0.329557 1_1:-75519165 Msa0044050:CDS
AAAGAAAGGAAACTTGTTAT+AGG 0.331567 1_1:-75518897 Msa0044050:three_prime_UTR
CCCTGATGACTCTTACATTC+TGG 0.354348 1_1:-75519249 Msa0044050:CDS
GCACCCGCTCTGCATGAATA+AGG 0.356204 1_1:+75519197 None:intergenic
GTTGCCAGTTGTTAATGATA+TGG 0.392583 1_1:-75518801 Msa0044050:three_prime_UTR
TGTGCTGGCCAAGTGGTTAC+GGG 0.411836 1_1:-75519164 Msa0044050:CDS
AAGAATGTGTCCAAAACATT+TGG 0.421132 1_1:-75519362 Msa0044050:CDS
AATTCGTTCTCTGTTCCATC+TGG 0.449513 1_1:+75519278 None:intergenic
TCCAGAATGTAAGAGTCATC+AGG 0.451621 1_1:+75519248 None:intergenic
TTGACTATGTGTCATGTAGC+TGG 0.452024 1_1:-75518974 Msa0044050:three_prime_UTR
CTTTCAACAGCTTCATTGTA+CGG 0.469663 1_1:-75519446 Msa0044050:CDS
CACACCATATCATTAACAAC+TGG 0.471108 1_1:+75518797 None:intergenic
CTGGATTCAGCTGAAGATGC+TGG 0.504908 1_1:-75519230 Msa0044050:CDS
GGTGCATGTTCTACTTGTGC+TGG 0.505031 1_1:-75519179 Msa0044050:CDS
GTATACAAAGTGAAACTGAT+TGG 0.509553 1_1:-75519302 Msa0044050:CDS
TAATGATATGGTGTGTAACT+TGG 0.510456 1_1:-75518789 Msa0044050:three_prime_UTR
CTACCTTATTCATGCAGAGC+GGG 0.513286 1_1:-75519200 Msa0044050:CDS
CGACAGAACCCGTAACCACT+TGG 0.534235 1_1:+75519156 None:intergenic
GTGAAACTGATTGGACCAGA+TGG 0.549003 1_1:-75519293 Msa0044050:CDS
TGTACGGAACAGTGCCCTCT+CGG 0.555453 1_1:-75519430 Msa0044050:CDS
CGATGAGCAGCAAATCGAGA+AGG 0.589494 1_1:-75519111 Msa0044050:CDS
CCAGAATGTAAGAGTCATCA+GGG 0.593554 1_1:+75519249 None:intergenic
TCTCACGGTGGACATATTCA+CGG 0.595716 1_1:+75519469 None:intergenic
ATCATTTAGAGTATCTGCAA+TGG 0.596293 1_1:-75519327 Msa0044050:CDS
TGGTTGTTGTGAAACTATGT+TGG 0.601901 1_1:-75518954 Msa0044050:three_prime_UTR
TTCTACTTGTGCTGGCCAAG+TGG 0.605670 1_1:-75519171 Msa0044050:CDS
TTCAGCTGAAGATGCTGGAG+TGG 0.623617 1_1:-75519225 Msa0044050:CDS
GATGAGCAGCAAATCGAGAA+GGG 0.629753 1_1:-75519110 Msa0044050:CDS
TACTGTGATTTACACACACA+AGG 0.647153 1_1:-75519051 Msa0044050:CDS
TCTCGATTTGCTGCTCATCG+AGG 0.652018 1_1:+75519114 None:intergenic
ACACATAGTCAAAACATGCA+CGG 0.652038 1_1:+75518985 None:intergenic
TGAAGCTGTTGAAAGTCTCA+CGG 0.659563 1_1:+75519454 None:intergenic
AGAGCACAAGATGTCCGAGA+GGG 0.687025 1_1:+75519416 None:intergenic
GCTACCTTATTCATGCAGAG+CGG 0.717700 1_1:-75519201 Msa0044050:CDS
TGTGATTTACACACACAAGG+AGG 0.721823 1_1:-75519048 Msa0044050:CDS
CAGAGCACAAGATGTCCGAG+AGG 0.730685 1_1:+75519415 None:intergenic
CAGAATGTAAGAGTCATCAG+GGG 0.754480 1_1:+75519250 None:intergenic
AGCTGTTGAAAGTCTCACGG+TGG 0.759008 1_1:+75519457 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATTTTAATTGATTCAA+TGG - chr1_1:75519143-75519162 Msa0044050:CDS 10.0%
!!! AATTGTAAAACATTTTTATA+AGG + chr1_1:75519509-75519528 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTGGAA+TGG + chr1_1:75520277-75520296 None:intergenic 10.0%
!!! ACAAGTTTTTTTTTTTTTTT+TGG + chr1_1:75520282-75520301 None:intergenic 10.0%
!! AAAAAAAAAAAAAAAACTTG+TGG - chr1_1:75520281-75520300 Msa0044050:intron 10.0%
!!! ATCTATATTTATTTTTATTC+AGG - chr1_1:75520420-75520439 Msa0044050:intron 10.0%
!!! AATTATTTTAATTGATTCAA+TGG - chr1_1:75519143-75519162 Msa0044050:CDS 10.0%
!!! AATTGTAAAACATTTTTATA+AGG + chr1_1:75519509-75519528 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTGGAA+TGG + chr1_1:75520277-75520296 None:intergenic 10.0%
!!! ACAAGTTTTTTTTTTTTTTT+TGG + chr1_1:75520282-75520301 None:intergenic 10.0%
!! AAAAAAAAAAAAAAAACTTG+TGG - chr1_1:75520281-75520300 Msa0044050:intron 10.0%
!!! ATCTATATTTATTTTTATTC+AGG - chr1_1:75520420-75520439 Msa0044050:intron 10.0%
!! AATAGTTAATAAGGATAATT+TGG + chr1_1:75519645-75519664 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr1_1:75519644-75519663 Msa0044050:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr1_1:75519771-75519790 Msa0044050:intron 15.0%
!! ATAAATATCATTATGATGAT+GGG - chr1_1:75519951-75519970 Msa0044050:intron 15.0%
!!! TTTTCTATAATTACTATTAC+TGG - chr1_1:75521063-75521082 Msa0044050:intron 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr1_1:75519645-75519664 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr1_1:75519644-75519663 Msa0044050:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr1_1:75519771-75519790 Msa0044050:intron 15.0%
!! ATAAATATCATTATGATGAT+GGG - chr1_1:75519951-75519970 Msa0044050:intron 15.0%
!!! TTTTCTATAATTACTATTAC+TGG - chr1_1:75521063-75521082 Msa0044050:intron 15.0%
!! AATAACATAGGTTATAAACT+TGG + chr1_1:75519184-75519203 None:intergenic 20.0%
!!! TGGTGTTTTTTTGTTTTTTT+TGG - chr1_1:75519287-75519306 Msa0044050:CDS 20.0%
!!! TGTAAAACATTTTTATAAGG+AGG + chr1_1:75519506-75519525 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr1_1:75519654-75519673 None:intergenic 20.0%
!!! TAGTTTTAAAAGTAATGCTT+TGG - chr1_1:75519668-75519687 Msa0044050:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr1_1:75519731-75519750 Msa0044050:intron 20.0%
!! CATAAATATCATTATGATGA+TGG - chr1_1:75519950-75519969 Msa0044050:intron 20.0%
!! TATATAAATAACCTCCAAAA+CGG + chr1_1:75520244-75520263 None:intergenic 20.0%
!!! TCATTTTAGGAAATAAAGAA+AGG - chr1_1:75521015-75521034 Msa0044050:intron 20.0%
!! AATAACATAGGTTATAAACT+TGG + chr1_1:75519184-75519203 None:intergenic 20.0%
!!! TGGTGTTTTTTTGTTTTTTT+TGG - chr1_1:75519287-75519306 Msa0044050:CDS 20.0%
!!! TGTAAAACATTTTTATAAGG+AGG + chr1_1:75519506-75519525 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr1_1:75519654-75519673 None:intergenic 20.0%
!!! TAGTTTTAAAAGTAATGCTT+TGG - chr1_1:75519668-75519687 Msa0044050:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr1_1:75519731-75519750 Msa0044050:intron 20.0%
!! CATAAATATCATTATGATGA+TGG - chr1_1:75519950-75519969 Msa0044050:intron 20.0%
!! TATATAAATAACCTCCAAAA+CGG + chr1_1:75520244-75520263 None:intergenic 20.0%
!!! TCATTTTAGGAAATAAAGAA+AGG - chr1_1:75521015-75521034 Msa0044050:intron 20.0%
! GTGAATCAATCATTTGATTA+TGG - chr1_1:75518790-75518809 Msa0044050:three_prime_UTR 25.0%
! TCATTTGATTATGGAAATGA+TGG - chr1_1:75518799-75518818 Msa0044050:three_prime_UTR 25.0%
!!! TCGTCGTAGTTTTTTATATA+AGG - chr1_1:75518834-75518853 Msa0044050:three_prime_UTR 25.0%
! TAGAAAAAGAGATAAGACAA+TGG - chr1_1:75518947-75518966 Msa0044050:three_prime_UTR 25.0%
! ATGAGAGAATGAAATAACAT+AGG + chr1_1:75519196-75519215 None:intergenic 25.0%
! GTAAAAATACAGTATAGCTA+AGG + chr1_1:75519360-75519379 None:intergenic 25.0%
! AATCAGATAAGTACCAAATA+CGG + chr1_1:75519384-75519403 None:intergenic 25.0%
! TGTATCTTATTAACAATCAC+TGG + chr1_1:75519407-75519426 None:intergenic 25.0%
!!! AACTTTTTTGTCAACTTCTA+CGG + chr1_1:75519532-75519551 None:intergenic 25.0%
! AATGATTAAGAGAAAGAGAA+TGG - chr1_1:75519611-75519630 Msa0044050:intron 25.0%
! AGTAATACTTAAAGCTAAGT+AGG - chr1_1:75519824-75519843 Msa0044050:intron 25.0%
! AAAGGCAATTTAAAGAATCA+AGG + chr1_1:75520012-75520031 None:intergenic 25.0%
! AAAGAAATCATGTGTATCAA+AGG + chr1_1:75520030-75520049 None:intergenic 25.0%
!!! TCTATTCTTGTGTTTTGTTA+TGG - chr1_1:75520065-75520084 Msa0044050:intron 25.0%
! ATCAAAAGAAAAACTTGTGT+AGG - chr1_1:75520108-75520127 Msa0044050:intron 25.0%
! AATTTCTTGTAAGAACTTTC+AGG - chr1_1:75520309-75520328 Msa0044050:intron 25.0%
! ATTTCTTGTAAGAACTTTCA+GGG - chr1_1:75520310-75520329 Msa0044050:intron 25.0%
!!! AGATTTTAGTCCAAATGTTT+TGG + chr1_1:75520577-75520596 None:intergenic 25.0%
!! AAATCACAGTATCTGATTTT+GGG + chr1_1:75520866-75520885 None:intergenic 25.0%
!! TAAATCACAGTATCTGATTT+TGG + chr1_1:75520867-75520886 None:intergenic 25.0%
!! GTAACTTACTGAATCATTTT+AGG - chr1_1:75521002-75521021 Msa0044050:intron 25.0%
!!! AAAGAAAGGAAACTTGTTAT+AGG - chr1_1:75521029-75521048 Msa0044050:intron 25.0%
! GTGAATCAATCATTTGATTA+TGG - chr1_1:75518790-75518809 Msa0044050:three_prime_UTR 25.0%
! TCATTTGATTATGGAAATGA+TGG - chr1_1:75518799-75518818 Msa0044050:three_prime_UTR 25.0%
!!! TCGTCGTAGTTTTTTATATA+AGG - chr1_1:75518834-75518853 Msa0044050:three_prime_UTR 25.0%
! TAGAAAAAGAGATAAGACAA+TGG - chr1_1:75518947-75518966 Msa0044050:three_prime_UTR 25.0%
! ATGAGAGAATGAAATAACAT+AGG + chr1_1:75519196-75519215 None:intergenic 25.0%
! GTAAAAATACAGTATAGCTA+AGG + chr1_1:75519360-75519379 None:intergenic 25.0%
! AATCAGATAAGTACCAAATA+CGG + chr1_1:75519384-75519403 None:intergenic 25.0%
! TGTATCTTATTAACAATCAC+TGG + chr1_1:75519407-75519426 None:intergenic 25.0%
!!! AACTTTTTTGTCAACTTCTA+CGG + chr1_1:75519532-75519551 None:intergenic 25.0%
! AATGATTAAGAGAAAGAGAA+TGG - chr1_1:75519611-75519630 Msa0044050:intron 25.0%
! AGTAATACTTAAAGCTAAGT+AGG - chr1_1:75519824-75519843 Msa0044050:intron 25.0%
! AAAGGCAATTTAAAGAATCA+AGG + chr1_1:75520012-75520031 None:intergenic 25.0%
! AAAGAAATCATGTGTATCAA+AGG + chr1_1:75520030-75520049 None:intergenic 25.0%
!!! TCTATTCTTGTGTTTTGTTA+TGG - chr1_1:75520065-75520084 Msa0044050:intron 25.0%
! ATCAAAAGAAAAACTTGTGT+AGG - chr1_1:75520108-75520127 Msa0044050:intron 25.0%
! AATTTCTTGTAAGAACTTTC+AGG - chr1_1:75520309-75520328 Msa0044050:intron 25.0%
! ATTTCTTGTAAGAACTTTCA+GGG - chr1_1:75520310-75520329 Msa0044050:intron 25.0%
!!! AGATTTTAGTCCAAATGTTT+TGG + chr1_1:75520577-75520596 None:intergenic 25.0%
!! AAATCACAGTATCTGATTTT+GGG + chr1_1:75520866-75520885 None:intergenic 25.0%
!! TAAATCACAGTATCTGATTT+TGG + chr1_1:75520867-75520886 None:intergenic 25.0%
!! GTAACTTACTGAATCATTTT+AGG - chr1_1:75521002-75521021 Msa0044050:intron 25.0%
!!! AAAGAAAGGAAACTTGTTAT+AGG - chr1_1:75521029-75521048 Msa0044050:intron 25.0%
AAGATAAAAGGGAAATGGAA+AGG + chr1_1:75518890-75518909 None:intergenic 30.0%
ATCTCACAGTGAAGATAAAA+GGG + chr1_1:75518901-75518920 None:intergenic 30.0%
AATCTCACAGTGAAGATAAA+AGG + chr1_1:75518902-75518921 None:intergenic 30.0%
! TAATTGATTCAATGGTACAC+TGG - chr1_1:75519151-75519170 Msa0044050:CDS 30.0%
TATTTCATTCTCTCATCCTA+TGG - chr1_1:75519200-75519219 Msa0044050:CDS 30.0%
!!! CTATGGTACTTTTTTTGACT+GGG - chr1_1:75519217-75519236 Msa0044050:CDS 30.0%
!!! TATGGTACTTTTTTTGACTG+GGG - chr1_1:75519218-75519237 Msa0044050:CDS 30.0%
!! CTGTATTTTTACACCGTATT+TGG - chr1_1:75519368-75519387 Msa0044050:CDS 30.0%
TTTATCCACTTCAGGAATTA+GGG + chr1_1:75519450-75519469 None:intergenic 30.0%
! TTTTATCCACTTCAGGAATT+AGG + chr1_1:75519451-75519470 None:intergenic 30.0%
TAATTCCTGAAGTGGATAAA+AGG - chr1_1:75519450-75519469 Msa0044050:CDS 30.0%
AATTCCTGAAGTGGATAAAA+GGG - chr1_1:75519451-75519470 Msa0044050:CDS 30.0%
!! AAACGACACTCATTTTGAAA+CGG + chr1_1:75519562-75519581 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - chr1_1:75519563-75519582 Msa0044050:intron 30.0%
! TTTTATCCTCTATGCGTATT+TGG - chr1_1:75520143-75520162 Msa0044050:intron 30.0%
TAAAAACCAAATACGCATAG+AGG + chr1_1:75520152-75520171 None:intergenic 30.0%
TTTCTTGTAAGAACTTTCAG+GGG - chr1_1:75520311-75520330 Msa0044050:intron 30.0%
AAGAATGTGTCCAAAACATT+TGG - chr1_1:75520564-75520583 Msa0044050:intron 30.0%
! ATCATTTAGAGTATCTGCAA+TGG - chr1_1:75520599-75520618 Msa0044050:intron 30.0%
GTATACAAAGTGAAACTGAT+TGG - chr1_1:75520624-75520643 Msa0044050:intron 30.0%
TAATGATATGGTGTGTAACT+TGG - chr1_1:75521137-75521156 Msa0044050:intron 30.0%
! AATGATTGATTCACTTTCCA+TGG + chr1_1:75518784-75518803 None:intergenic 30.0%
AAGATAAAAGGGAAATGGAA+AGG + chr1_1:75518890-75518909 None:intergenic 30.0%
ATCTCACAGTGAAGATAAAA+GGG + chr1_1:75518901-75518920 None:intergenic 30.0%
AATCTCACAGTGAAGATAAA+AGG + chr1_1:75518902-75518921 None:intergenic 30.0%
! TAATTGATTCAATGGTACAC+TGG - chr1_1:75519151-75519170 Msa0044050:CDS 30.0%
TATTTCATTCTCTCATCCTA+TGG - chr1_1:75519200-75519219 Msa0044050:CDS 30.0%
!!! CTATGGTACTTTTTTTGACT+GGG - chr1_1:75519217-75519236 Msa0044050:CDS 30.0%
!!! TATGGTACTTTTTTTGACTG+GGG - chr1_1:75519218-75519237 Msa0044050:CDS 30.0%
!! CTGTATTTTTACACCGTATT+TGG - chr1_1:75519368-75519387 Msa0044050:CDS 30.0%
TTTATCCACTTCAGGAATTA+GGG + chr1_1:75519450-75519469 None:intergenic 30.0%
! TTTTATCCACTTCAGGAATT+AGG + chr1_1:75519451-75519470 None:intergenic 30.0%
TAATTCCTGAAGTGGATAAA+AGG - chr1_1:75519450-75519469 Msa0044050:CDS 30.0%
AATTCCTGAAGTGGATAAAA+GGG - chr1_1:75519451-75519470 Msa0044050:CDS 30.0%
!! AAACGACACTCATTTTGAAA+CGG + chr1_1:75519562-75519581 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - chr1_1:75519563-75519582 Msa0044050:intron 30.0%
! TTTTATCCTCTATGCGTATT+TGG - chr1_1:75520143-75520162 Msa0044050:intron 30.0%
TAAAAACCAAATACGCATAG+AGG + chr1_1:75520152-75520171 None:intergenic 30.0%
TTTCTTGTAAGAACTTTCAG+GGG - chr1_1:75520311-75520330 Msa0044050:intron 30.0%
AAGAATGTGTCCAAAACATT+TGG - chr1_1:75520564-75520583 Msa0044050:intron 30.0%
! ATCATTTAGAGTATCTGCAA+TGG - chr1_1:75520599-75520618 Msa0044050:intron 30.0%
GTATACAAAGTGAAACTGAT+TGG - chr1_1:75520624-75520643 Msa0044050:intron 30.0%
TAATGATATGGTGTGTAACT+TGG - chr1_1:75521137-75521156 Msa0044050:intron 30.0%
CAGTGAAGATAAAAGGGAAA+TGG + chr1_1:75518895-75518914 None:intergenic 35.0%
ATCTTCACTGTGAGATTTGT+AGG - chr1_1:75518905-75518924 Msa0044050:three_prime_UTR 35.0%
TCTTCACTGTGAGATTTGTA+GGG - chr1_1:75518906-75518925 Msa0044050:three_prime_UTR 35.0%
AAGACAATGGCGTAAATTGA+AGG - chr1_1:75518960-75518979 Msa0044050:three_prime_UTR 35.0%
AATGGCGTAAATTGAAGGTA+AGG - chr1_1:75518965-75518984 Msa0044050:three_prime_UTR 35.0%
AGAACAAGGATGGTTTAGTA+GGG - chr1_1:75519042-75519061 Msa0044050:CDS 35.0%
!! CCAGTCAAAAAAAGTACCAT+AGG + chr1_1:75519219-75519238 None:intergenic 35.0%
!!! CCTATGGTACTTTTTTTGAC+TGG - chr1_1:75519216-75519235 Msa0044050:CDS 35.0%
! ATGTATTTTACCGATACTGC+TGG - chr1_1:75519267-75519286 Msa0044050:CDS 35.0%
CAAAAAAACACCAGCAGTAT+CGG + chr1_1:75519280-75519299 None:intergenic 35.0%
AAGATACAACTTCTGACAAC+CGG - chr1_1:75519419-75519438 Msa0044050:CDS 35.0%
! TTTTCCCTTTTATCCACTTC+AGG + chr1_1:75519458-75519477 None:intergenic 35.0%
GTAGTGTGCATGGTATTAAT+TGG - chr1_1:75519693-75519712 Msa0044050:intron 35.0%
TGTGCATGGTATTAATTGGA+GGG - chr1_1:75519697-75519716 Msa0044050:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr1_1:75519761-75519780 Msa0044050:intron 35.0%
!! TTCTTGTGTTTTGTTATGGC+TGG - chr1_1:75520069-75520088 Msa0044050:intron 35.0%
! TATGGCTGGATTTGCTTATT+TGG - chr1_1:75520083-75520102 Msa0044050:intron 35.0%
! ATGGCTGGATTTGCTTATTT+GGG - chr1_1:75520084-75520103 Msa0044050:intron 35.0%
GAAAAACTTGTGTAGGATTG+TGG - chr1_1:75520115-75520134 Msa0044050:intron 35.0%
TAAATAACCTCCAAAACGGA+TGG + chr1_1:75520240-75520259 None:intergenic 35.0%
CTTTCAACAGCTTCATTGTA+CGG - chr1_1:75520480-75520499 Msa0044050:intron 35.0%
TTCACAGATCTCAAACTTGA+TGG + chr1_1:75520546-75520565 None:intergenic 35.0%
TACTGTGATTTACACACACA+AGG - chr1_1:75520875-75520894 Msa0044050:intron 35.0%
ACACATAGTCAAAACATGCA+CGG + chr1_1:75520944-75520963 None:intergenic 35.0%
TGGTTGTTGTGAAACTATGT+TGG - chr1_1:75520972-75520991 Msa0044050:intron 35.0%
GTTGCCAGTTGTTAATGATA+TGG - chr1_1:75521125-75521144 Msa0044050:intron 35.0%
CACACCATATCATTAACAAC+TGG + chr1_1:75521132-75521151 None:intergenic 35.0%
CAGTGAAGATAAAAGGGAAA+TGG + chr1_1:75518895-75518914 None:intergenic 35.0%
ATCTTCACTGTGAGATTTGT+AGG - chr1_1:75518905-75518924 Msa0044050:three_prime_UTR 35.0%
TCTTCACTGTGAGATTTGTA+GGG - chr1_1:75518906-75518925 Msa0044050:three_prime_UTR 35.0%
AAGACAATGGCGTAAATTGA+AGG - chr1_1:75518960-75518979 Msa0044050:three_prime_UTR 35.0%
AATGGCGTAAATTGAAGGTA+AGG - chr1_1:75518965-75518984 Msa0044050:three_prime_UTR 35.0%
AGAACAAGGATGGTTTAGTA+GGG - chr1_1:75519042-75519061 Msa0044050:CDS 35.0%
!! CCAGTCAAAAAAAGTACCAT+AGG + chr1_1:75519219-75519238 None:intergenic 35.0%
!!! CCTATGGTACTTTTTTTGAC+TGG - chr1_1:75519216-75519235 Msa0044050:CDS 35.0%
! ATGTATTTTACCGATACTGC+TGG - chr1_1:75519267-75519286 Msa0044050:CDS 35.0%
CAAAAAAACACCAGCAGTAT+CGG + chr1_1:75519280-75519299 None:intergenic 35.0%
AAGATACAACTTCTGACAAC+CGG - chr1_1:75519419-75519438 Msa0044050:CDS 35.0%
! TTTTCCCTTTTATCCACTTC+AGG + chr1_1:75519458-75519477 None:intergenic 35.0%
GTAGTGTGCATGGTATTAAT+TGG - chr1_1:75519693-75519712 Msa0044050:intron 35.0%
TGTGCATGGTATTAATTGGA+GGG - chr1_1:75519697-75519716 Msa0044050:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr1_1:75519761-75519780 Msa0044050:intron 35.0%
!! TTCTTGTGTTTTGTTATGGC+TGG - chr1_1:75520069-75520088 Msa0044050:intron 35.0%
! TATGGCTGGATTTGCTTATT+TGG - chr1_1:75520083-75520102 Msa0044050:intron 35.0%
! ATGGCTGGATTTGCTTATTT+GGG - chr1_1:75520084-75520103 Msa0044050:intron 35.0%
GAAAAACTTGTGTAGGATTG+TGG - chr1_1:75520115-75520134 Msa0044050:intron 35.0%
TAAATAACCTCCAAAACGGA+TGG + chr1_1:75520240-75520259 None:intergenic 35.0%
CTTTCAACAGCTTCATTGTA+CGG - chr1_1:75520480-75520499 Msa0044050:intron 35.0%
TTCACAGATCTCAAACTTGA+TGG + chr1_1:75520546-75520565 None:intergenic 35.0%
TACTGTGATTTACACACACA+AGG - chr1_1:75520875-75520894 Msa0044050:intron 35.0%
ACACATAGTCAAAACATGCA+CGG + chr1_1:75520944-75520963 None:intergenic 35.0%
TGGTTGTTGTGAAACTATGT+TGG - chr1_1:75520972-75520991 Msa0044050:intron 35.0%
GTTGCCAGTTGTTAATGATA+TGG - chr1_1:75521125-75521144 Msa0044050:intron 35.0%
CACACCATATCATTAACAAC+TGG + chr1_1:75521132-75521151 None:intergenic 35.0%
CAAGTGGTTCATAGAGAACA+AGG - chr1_1:75519028-75519047 Msa0044050:exon 40.0%
TGGTTCATAGAGAACAAGGA+TGG - chr1_1:75519032-75519051 Msa0044050:exon 40.0%
GAGAACAAGGATGGTTTAGT+AGG - chr1_1:75519041-75519060 Msa0044050:CDS 40.0%
GAACAAGGATGGTTTAGTAG+GGG - chr1_1:75519043-75519062 Msa0044050:CDS 40.0%
AGTAGGGGAAGAGATAAACA+AGG - chr1_1:75519058-75519077 Msa0044050:CDS 40.0%
AGAGATAAACAAGGAGGGAT+TGG - chr1_1:75519067-75519086 Msa0044050:CDS 40.0%
GAGATAAACAAGGAGGGATT+GGG - chr1_1:75519068-75519087 Msa0044050:CDS 40.0%
ATTCAATGGTACACTGGTGA+GGG - chr1_1:75519157-75519176 Msa0044050:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr1_1:75519801-75519820 None:intergenic 40.0%
GTGTGCATGGTATTAATTGG+AGG - chr1_1:75519696-75519715 Msa0044050:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr1_1:75519801-75519820 Msa0044050:intron 40.0%
AGTGATGCAAAGAAAACCTG+AGG - chr1_1:75519850-75519869 Msa0044050:intron 40.0%
GTGATGCAAAGAAAACCTGA+GGG - chr1_1:75519851-75519870 Msa0044050:intron 40.0%
CATGCACAATGACTTACAAG+TGG + chr1_1:75519977-75519996 None:intergenic 40.0%
! TGGCTGGATTTGCTTATTTG+GGG - chr1_1:75520085-75520104 Msa0044050:intron 40.0%
! GTACTTGATTCCATCCGTTT+TGG - chr1_1:75520227-75520246 Msa0044050:intron 40.0%
TGAAGCTGTTGAAAGTCTCA+CGG + chr1_1:75520475-75520494 None:intergenic 40.0%
AATTCGTTCTCTGTTCCATC+TGG + chr1_1:75520651-75520670 None:intergenic 40.0%
CAGAATGTAAGAGTCATCAG+GGG + chr1_1:75520679-75520698 None:intergenic 40.0%
CCAGAATGTAAGAGTCATCA+GGG + chr1_1:75520680-75520699 None:intergenic 40.0%
TCCAGAATGTAAGAGTCATC+AGG + chr1_1:75520681-75520700 None:intergenic 40.0%
TGTGATTTACACACACAAGG+AGG - chr1_1:75520878-75520897 Msa0044050:intron 40.0%
TTGACTATGTGTCATGTAGC+TGG - chr1_1:75520952-75520971 Msa0044050:intron 40.0%
CAAGTGGTTCATAGAGAACA+AGG - chr1_1:75519028-75519047 Msa0044050:exon 40.0%
TGGTTCATAGAGAACAAGGA+TGG - chr1_1:75519032-75519051 Msa0044050:exon 40.0%
GAGAACAAGGATGGTTTAGT+AGG - chr1_1:75519041-75519060 Msa0044050:CDS 40.0%
GAACAAGGATGGTTTAGTAG+GGG - chr1_1:75519043-75519062 Msa0044050:CDS 40.0%
AGTAGGGGAAGAGATAAACA+AGG - chr1_1:75519058-75519077 Msa0044050:CDS 40.0%
AGAGATAAACAAGGAGGGAT+TGG - chr1_1:75519067-75519086 Msa0044050:CDS 40.0%
GAGATAAACAAGGAGGGATT+GGG - chr1_1:75519068-75519087 Msa0044050:CDS 40.0%
ATTCAATGGTACACTGGTGA+GGG - chr1_1:75519157-75519176 Msa0044050:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr1_1:75519801-75519820 None:intergenic 40.0%
GTGTGCATGGTATTAATTGG+AGG - chr1_1:75519696-75519715 Msa0044050:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr1_1:75519801-75519820 Msa0044050:intron 40.0%
AGTGATGCAAAGAAAACCTG+AGG - chr1_1:75519850-75519869 Msa0044050:intron 40.0%
GTGATGCAAAGAAAACCTGA+GGG - chr1_1:75519851-75519870 Msa0044050:intron 40.0%
CATGCACAATGACTTACAAG+TGG + chr1_1:75519977-75519996 None:intergenic 40.0%
! TGGCTGGATTTGCTTATTTG+GGG - chr1_1:75520085-75520104 Msa0044050:intron 40.0%
! GTACTTGATTCCATCCGTTT+TGG - chr1_1:75520227-75520246 Msa0044050:intron 40.0%
TGAAGCTGTTGAAAGTCTCA+CGG + chr1_1:75520475-75520494 None:intergenic 40.0%
AATTCGTTCTCTGTTCCATC+TGG + chr1_1:75520651-75520670 None:intergenic 40.0%
CAGAATGTAAGAGTCATCAG+GGG + chr1_1:75520679-75520698 None:intergenic 40.0%
CCAGAATGTAAGAGTCATCA+GGG + chr1_1:75520680-75520699 None:intergenic 40.0%
TCCAGAATGTAAGAGTCATC+AGG + chr1_1:75520681-75520700 None:intergenic 40.0%
TGTGATTTACACACACAAGG+AGG - chr1_1:75520878-75520897 Msa0044050:intron 40.0%
TTGACTATGTGTCATGTAGC+TGG - chr1_1:75520952-75520971 Msa0044050:intron 40.0%
AGACATGTTGAGTCTGCAAG+TGG - chr1_1:75519012-75519031 Msa0044050:three_prime_UTR 45.0%
AGGGGAAGAGATAAACAAGG+AGG - chr1_1:75519061-75519080 Msa0044050:CDS 45.0%
GGGGAAGAGATAAACAAGGA+GGG - chr1_1:75519062-75519081 Msa0044050:CDS 45.0%
GATTCAATGGTACACTGGTG+AGG - chr1_1:75519156-75519175 Msa0044050:CDS 45.0%
!! TTTTGACTGGGGTAGAAGAG+TGG - chr1_1:75519229-75519248 Msa0044050:CDS 45.0%
TTCAGGAATTAGGGTGACTC+CGG + chr1_1:75519441-75519460 None:intergenic 45.0%
AGTCACCCTAATTCCTGAAG+TGG - chr1_1:75519442-75519461 Msa0044050:CDS 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr1_1:75519800-75519819 None:intergenic 45.0%
!! TGCTTTGGAAGTAGTGTGCA+TGG - chr1_1:75519683-75519702 Msa0044050:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr1_1:75519800-75519819 Msa0044050:intron 45.0%
CTAAGCAATTTCGTACCCTC+AGG + chr1_1:75519869-75519888 None:intergenic 45.0%
! CTGAGGGTACGAAATTGCTT+AGG - chr1_1:75519867-75519886 Msa0044050:intron 45.0%
GTACAACATCCTTGAGCACA+AGG + chr1_1:75520211-75520230 None:intergenic 45.0%
! CTTGATTCCATCCGTTTTGG+AGG - chr1_1:75520230-75520249 Msa0044050:intron 45.0%
TCTCACGGTGGACATATTCA+CGG + chr1_1:75520460-75520479 None:intergenic 45.0%
GTGAAACTGATTGGACCAGA+TGG - chr1_1:75520633-75520652 Msa0044050:intron 45.0%
CCCTGATGACTCTTACATTC+TGG - chr1_1:75520677-75520696 Msa0044050:intron 45.0%
GCTACCTTATTCATGCAGAG+CGG - chr1_1:75520725-75520744 Msa0044050:intron 45.0%
CTACCTTATTCATGCAGAGC+GGG - chr1_1:75520726-75520745 Msa0044050:intron 45.0%
GATGAGCAGCAAATCGAGAA+GGG - chr1_1:75520816-75520835 Msa0044050:intron 45.0%
AGACATGTTGAGTCTGCAAG+TGG - chr1_1:75519012-75519031 Msa0044050:three_prime_UTR 45.0%
AGGGGAAGAGATAAACAAGG+AGG - chr1_1:75519061-75519080 Msa0044050:CDS 45.0%
GGGGAAGAGATAAACAAGGA+GGG - chr1_1:75519062-75519081 Msa0044050:CDS 45.0%
GATTCAATGGTACACTGGTG+AGG - chr1_1:75519156-75519175 Msa0044050:CDS 45.0%
!! TTTTGACTGGGGTAGAAGAG+TGG - chr1_1:75519229-75519248 Msa0044050:CDS 45.0%
TTCAGGAATTAGGGTGACTC+CGG + chr1_1:75519441-75519460 None:intergenic 45.0%
AGTCACCCTAATTCCTGAAG+TGG - chr1_1:75519442-75519461 Msa0044050:CDS 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr1_1:75519800-75519819 None:intergenic 45.0%
!! TGCTTTGGAAGTAGTGTGCA+TGG - chr1_1:75519683-75519702 Msa0044050:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr1_1:75519800-75519819 Msa0044050:intron 45.0%
CTAAGCAATTTCGTACCCTC+AGG + chr1_1:75519869-75519888 None:intergenic 45.0%
! CTGAGGGTACGAAATTGCTT+AGG - chr1_1:75519867-75519886 Msa0044050:intron 45.0%
GTACAACATCCTTGAGCACA+AGG + chr1_1:75520211-75520230 None:intergenic 45.0%
! CTTGATTCCATCCGTTTTGG+AGG - chr1_1:75520230-75520249 Msa0044050:intron 45.0%
TCTCACGGTGGACATATTCA+CGG + chr1_1:75520460-75520479 None:intergenic 45.0%
GTGAAACTGATTGGACCAGA+TGG - chr1_1:75520633-75520652 Msa0044050:intron 45.0%
CCCTGATGACTCTTACATTC+TGG - chr1_1:75520677-75520696 Msa0044050:intron 45.0%
GCTACCTTATTCATGCAGAG+CGG - chr1_1:75520725-75520744 Msa0044050:intron 45.0%
CTACCTTATTCATGCAGAGC+GGG - chr1_1:75520726-75520745 Msa0044050:intron 45.0%
GATGAGCAGCAAATCGAGAA+GGG - chr1_1:75520816-75520835 Msa0044050:intron 45.0%
AACAAGGAGGGATTGGGTGA+AGG - chr1_1:75519074-75519093 Msa0044050:CDS 50.0%
TCGAGTACTCCTTGTGCTCA+AGG - chr1_1:75520199-75520218 Msa0044050:intron 50.0%
AGCTGTTGAAAGTCTCACGG+TGG + chr1_1:75520472-75520491 None:intergenic 50.0%
AGAGCACAAGATGTCCGAGA+GGG + chr1_1:75520513-75520532 None:intergenic 50.0%
CTGGATTCAGCTGAAGATGC+TGG - chr1_1:75520696-75520715 Msa0044050:intron 50.0%
TTCAGCTGAAGATGCTGGAG+TGG - chr1_1:75520701-75520720 Msa0044050:intron 50.0%
GGTGCATGTTCTACTTGTGC+TGG - chr1_1:75520747-75520766 Msa0044050:intron 50.0%
! TTCTACTTGTGCTGGCCAAG+TGG - chr1_1:75520755-75520774 Msa0044050:intron 50.0%
! TTGTGCTGGCCAAGTGGTTA+CGG - chr1_1:75520761-75520780 Msa0044050:intron 50.0%
!! TCTCGATTTGCTGCTCATCG+AGG + chr1_1:75520815-75520834 None:intergenic 50.0%
CGATGAGCAGCAAATCGAGA+AGG - chr1_1:75520815-75520834 Msa0044050:intron 50.0%
AACAAGGAGGGATTGGGTGA+AGG - chr1_1:75519074-75519093 Msa0044050:CDS 50.0%
TCGAGTACTCCTTGTGCTCA+AGG - chr1_1:75520199-75520218 Msa0044050:intron 50.0%
AGCTGTTGAAAGTCTCACGG+TGG + chr1_1:75520472-75520491 None:intergenic 50.0%
AGAGCACAAGATGTCCGAGA+GGG + chr1_1:75520513-75520532 None:intergenic 50.0%
CTGGATTCAGCTGAAGATGC+TGG - chr1_1:75520696-75520715 Msa0044050:intron 50.0%
TTCAGCTGAAGATGCTGGAG+TGG - chr1_1:75520701-75520720 Msa0044050:intron 50.0%
GGTGCATGTTCTACTTGTGC+TGG - chr1_1:75520747-75520766 Msa0044050:intron 50.0%
! TTCTACTTGTGCTGGCCAAG+TGG - chr1_1:75520755-75520774 Msa0044050:intron 50.0%
! TTGTGCTGGCCAAGTGGTTA+CGG - chr1_1:75520761-75520780 Msa0044050:intron 50.0%
!! TCTCGATTTGCTGCTCATCG+AGG + chr1_1:75520815-75520834 None:intergenic 50.0%
CGATGAGCAGCAAATCGAGA+AGG - chr1_1:75520815-75520834 Msa0044050:intron 50.0%
TGTACGGAACAGTGCCCTCT+CGG - chr1_1:75520496-75520515 Msa0044050:intron 55.0%
CAGAGCACAAGATGTCCGAG+AGG + chr1_1:75520514-75520533 None:intergenic 55.0%
GCACCCGCTCTGCATGAATA+AGG + chr1_1:75520732-75520751 None:intergenic 55.0%
! TGTGCTGGCCAAGTGGTTAC+GGG - chr1_1:75520762-75520781 Msa0044050:intron 55.0%
CGACAGAACCCGTAACCACT+TGG + chr1_1:75520773-75520792 None:intergenic 55.0%
TGTACGGAACAGTGCCCTCT+CGG - chr1_1:75520496-75520515 Msa0044050:intron 55.0%
CAGAGCACAAGATGTCCGAG+AGG + chr1_1:75520514-75520533 None:intergenic 55.0%
GCACCCGCTCTGCATGAATA+AGG + chr1_1:75520732-75520751 None:intergenic 55.0%
! TGTGCTGGCCAAGTGGTTAC+GGG - chr1_1:75520762-75520781 Msa0044050:intron 55.0%
CGACAGAACCCGTAACCACT+TGG + chr1_1:75520773-75520792 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 75518783 75521165 75518783 ID=Msa0044050;Name=Msa0044050
chr1_1 mRNA 75518783 75521165 75518783 ID=Msa0044050-mRNA-1;Parent=Msa0044050;Name=Msa0044050-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0.5|0.5|1|1|2|251|159
chr1_1 exon 75518783 75519506 75518783 ID=Msa0044050-mRNA-1:exon:25497;Parent=Msa0044050-mRNA-1
chr1_1 exon 75521159 75521165 75521159 ID=Msa0044050-mRNA-1:exon:25496;Parent=Msa0044050-mRNA-1
chr1_1 CDS 75521159 75521165 75521159 ID=Msa0044050-mRNA-1:cds;Parent=Msa0044050-mRNA-1
chr1_1 CDS 75519034 75519506 75519034 ID=Msa0044050-mRNA-1:cds;Parent=Msa0044050-mRNA-1
chr1_1 three_prime_UTR 75518783 75519033 75518783 ID=Msa0044050-mRNA-1:three_prime_utr;Parent=Msa0044050-mRNA-1
Gene Sequence

>Msa0044050

ATGGAAAGTATAAAAATGTCAGCCGTGAATATGTCCACCGTGAGACTTTCAACAGCTTCATTGTACGGAACAGTGCCCTCTCGGACATCTTGTGCTCTGATGAAGAGTCCATCAAGTTTGAGATCTGTGAAGAATGTGTCCAAAACATTTGGACTAAAATCTTCATCATTTAGAGTATCTGCAATGGCAGTATACAAAGTGAAACTGATTGGACCAGATGGAACAGAGAACGAATTTGATGCCCCTGATGACTCTTACATTCTGGATTCAGCTGAAGATGCTGGAGTGGAGCTACCTTATTCATGCAGAGCGGGTGCATGTTCTACTTGTGCTGGCCAAGTGGTTACGGGTTCTGTCGATCAGTCTGATCAATCTTTCCTCGATGAGCAGCAAATCGAGAAGGGTTATCTTCTCACTTGTGTCTCATACCCAAAATCAGATACTGTGATTTACACACACAAGGAGGAAGAACTCTACTAA
Protein sequence

>Msa0044050

MESIKMSAVNMSTVRLSTASLYGTVPSRTSCALMKSPSSLRSVKNVSKTFGLKSSSFRVSAMAVYKVKLIGPDGTENEFDAPDDSYILDSAEDAGVELPYSCRAGACSTCAGQVVTGSVDQSDQSFLDEQQIEKGYLLTCVSYPKSDTVIYTHKEEELY*