AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1360180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360180 MtrunA17_Chr5g0405761 95.302 298 14 0 1 298 31 328 0.0 576
Msa1360180 MtrunA17_Chr1g0156481 40.333 300 153 9 17 298 12 303 8.24e-62 198
Msa1360180 MtrunA17_Chr1g0176881 31.915 188 112 3 16 200 18 192 1.22e-28 115
Msa1360180 MtrunA17_Chr1g0176881 32.031 128 87 0 18 145 191 318 2.25e-22 97.1
Msa1360180 MtrunA17_Chr3g0113081 28.475 295 181 6 3 294 366 633 6.79e-28 113
Msa1360180 MtrunA17_Chr3g0113081 30.047 213 135 4 3 205 164 372 5.23e-24 102
Msa1360180 MtrunA17_Chr3g0113081 34.959 123 80 0 19 141 7 129 5.39e-18 84.3
Msa1360180 MtrunA17_Chr1g0188491 47.154 123 62 3 173 294 6 126 9.53e-28 104
Msa1360180 MtrunA17_Chr1g0172741 28.205 312 196 9 1 288 4 311 1.74e-27 108
Msa1360180 MtrunA17_Chr2g0285481 27.885 312 197 8 1 288 4 311 9.51e-27 107
Msa1360180 MtrunA17_Chr8g0334551 27.987 318 192 9 3 288 5 317 4.24e-26 105
Msa1360180 MtrunA17_Chr3g0093841 37.190 121 76 0 22 142 191 311 1.35e-19 89.0
Msa1360180 MtrunA17_Chr3g0093841 31.092 119 82 0 23 141 27 145 6.01e-17 81.3
Msa1360180 MtrunA17_Chr3g0093841 23.826 298 193 4 23 288 518 813 7.94e-17 80.9
Msa1360180 MtrunA17_Chr3g0093841 31.707 123 82 1 22 142 351 473 1.34e-15 77.0
Msa1360180 MtrunA17_Chr8g0360271 23.404 282 202 5 23 290 13 294 7.38e-19 84.7
Msa1360180 MtrunA17_Chr7g0264841 22.973 296 195 9 1 279 11 290 2.64e-16 77.8
Msa1360180 MtrunA17_Chr8g0354231 20.775 284 206 6 23 291 22 301 2.21e-14 72.4
Msa1360180 MtrunA17_Chr5g0402821 26.014 296 181 9 20 280 16 308 1.51e-13 70.1
Msa1360180 MtrunA17_Chr8g0362511 24.752 303 171 10 20 283 68 352 1.77e-13 70.1
Msa1360180 MtrunA17_Chr3g0087491 26.429 280 187 8 27 288 1 279 6.33e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360180 AT2G24960.1 34.507 142 93 0 17 158 9 150 3.19e-29 117
Msa1360180 AT2G24960.1 34.848 132 86 0 17 148 166 297 6.16e-22 96.3
Msa1360180 AT2G24960.1 26.335 281 185 5 22 297 533 796 3.40e-21 94.4
Msa1360180 AT2G24960.1 27.322 183 110 1 2 161 306 488 5.46e-20 90.5
Msa1360180 AT2G24960.2 34.507 142 93 0 17 158 9 150 3.46e-29 117
Msa1360180 AT2G24960.2 31.250 160 110 0 2 161 306 465 1.86e-24 103
Msa1360180 AT2G24960.2 34.848 132 86 0 17 148 166 297 5.87e-22 96.3
Msa1360180 AT2G24960.2 26.335 281 185 5 22 297 510 773 3.34e-21 94.4
Msa1360180 AT5G05800.1 28.866 291 171 7 18 290 165 437 3.21e-28 113
Msa1360180 AT5G05800.1 29.268 123 87 0 20 142 4 126 8.95e-15 74.7
Msa1360180 AT5G05800.5 28.866 291 171 7 18 290 165 437 3.21e-28 113
Msa1360180 AT5G05800.5 29.268 123 87 0 20 142 4 126 8.95e-15 74.7
Msa1360180 AT5G05800.3 28.866 291 171 7 18 290 165 437 3.21e-28 113
Msa1360180 AT5G05800.3 29.268 123 87 0 20 142 4 126 8.95e-15 74.7
Msa1360180 AT5G05800.2 28.866 291 171 7 18 290 165 437 3.21e-28 113
Msa1360180 AT5G05800.2 29.268 123 87 0 20 142 4 126 8.95e-15 74.7
Msa1360180 AT5G05800.4 28.866 291 171 7 18 290 165 437 3.21e-28 113
Msa1360180 AT5G05800.4 29.268 123 87 0 20 142 4 126 8.95e-15 74.7
Msa1360180 AT4G02210.1 34.965 143 92 1 17 158 8 150 4.16e-26 107
Msa1360180 AT4G02210.1 25.448 279 181 3 16 291 179 433 7.09e-25 103
Msa1360180 AT4G02210.2 34.965 143 92 1 17 158 8 150 4.16e-26 107
Msa1360180 AT4G02210.2 25.448 279 181 3 16 291 179 433 7.09e-25 103
Msa1360180 AT3G11290.2 29.210 291 185 7 18 290 163 450 6.47e-24 101
Msa1360180 AT3G11290.2 26.347 167 106 3 20 186 4 153 8.60e-12 65.9
Msa1360180 AT3G11290.1 29.210 291 185 7 18 290 163 450 6.47e-24 101
Msa1360180 AT3G11290.1 26.347 167 106 3 20 186 4 153 8.60e-12 65.9
Msa1360180 AT2G19220.1 27.575 301 177 12 10 288 151 432 9.28e-18 83.6
Msa1360180 AT4G02550.1 28.986 138 97 1 23 159 22 159 2.00e-16 78.6
Msa1360180 AT4G02550.5 28.986 138 97 1 23 159 22 159 2.00e-16 78.6
Msa1360180 AT4G02550.3 28.986 138 97 1 23 159 37 174 2.58e-16 78.2
Msa1360180 AT4G02550.2 23.443 273 184 6 23 291 22 273 3.01e-15 74.7
Msa1360180 AT4G02550.4 23.443 273 184 6 23 291 22 273 3.01e-15 74.7
Msa1360180 AT3G11310.1 28.916 166 98 5 16 166 169 329 2.91e-12 67.4

Find 55 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTGCGAATGATGAAATT+TGG 0.147259 tig0016950:-21411 Msa1360180:CDS
GTTTATTCCCTTTGTGTACT+TGG 0.221915 tig0016950:+21617 None:intergenic
TGGTGGCTGTCCAATCTTTA+AGG 0.238961 tig0016950:-20993 Msa1360180:CDS
TGATGTGTGCTCAACAATTC+TGG 0.281107 tig0016950:+20881 None:intergenic
TTGATGAAGGTGACTTTGTT+TGG 0.332628 tig0016950:-21453 Msa1360180:CDS
TAAGATACTGGCAGATTTGA+TGG 0.340898 tig0016950:-21644 Msa1360180:CDS
TTTAGAGTTATGTTGCTCTA+TGG 0.360492 tig0016950:+20682 None:intergenic
AATTGCGAATGATGAAATTT+GGG 0.361414 tig0016950:-21410 Msa1360180:CDS
TGATGAAGGTGACTTTGTTT+GGG 0.364398 tig0016950:-21452 Msa1360180:CDS
CAAGTTTCTGTAGGTGTAGT+AGG 0.385419 tig0016950:+20797 None:intergenic
GCCTTCTTGATCTTGTAATT+CGG 0.395466 tig0016950:+21719 None:intergenic
GTACATAGCTCCTTAAAGAT+TGG 0.398875 tig0016950:+20983 None:intergenic
ATTTGTCTCTTCCTCATCCT+CGG 0.434458 tig0016950:+20838 None:intergenic
AAAGTCACCTTCATCAAGAA+TGG 0.436149 tig0016950:+21459 None:intergenic
CAGCATATCTGACTGTGTTA+AGG 0.442168 tig0016950:-20657 Msa1360180:CDS
TATGTTAGTTAAACTATAGA+TGG 0.445852 tig0016950:-21746 Msa1360180:exon
AGCATCGCTCACTAAGATAC+TGG 0.460192 tig0016950:-21656 Msa1360180:CDS
AGAGGGCGTAAAGGAGTTGA+TGG 0.467269 tig0016950:-20761 Msa1360180:CDS
AACATAAGTAATCGATAAAG+AGG 0.477231 tig0016950:+21763 None:intergenic
GTTTAACTTGGTTGCACCGC+AGG 0.484247 tig0016950:-20526 Msa1360180:CDS
AAAAGGATAAACGTTTAACT+TGG 0.500116 tig0016950:-20538 Msa1360180:CDS
CGACTACTACAAGTTTCTGT+AGG 0.502887 tig0016950:+20788 None:intergenic
GCATGGAAACATATTTGTGA+TGG 0.508692 tig0016950:-21571 Msa1360180:CDS
AATTCTGGACAAGGAGCTCT+AGG 0.508728 tig0016950:+20896 None:intergenic
TTCTCTGACGCAGCAACGAA+CGG 0.509181 tig0016950:-20956 Msa1360180:CDS
AACAGGTCTGAAATGGGATA+AGG 0.509493 tig0016950:-21536 Msa1360180:CDS
TTCATCTGAATCCGAGGATG+AGG 0.517272 tig0016950:-20849 Msa1360180:CDS
TGCTGTTGTATCCAATAGAT+AGG 0.518107 tig0016950:-20501 Msa1360180:exon
AAGGATTTAGATTTAATGCA+AGG 0.526331 tig0016950:-20638 Msa1360180:CDS
AAGTCACCTTCATCAAGAAT+GGG 0.528147 tig0016950:+21460 None:intergenic
AGTCGCAAAAGAGGGCGTAA+AGG 0.529428 tig0016950:-20770 Msa1360180:CDS
CTTTGTTTGGGATGAAGCCA+CGG 0.540715 tig0016950:-21440 Msa1360180:CDS
ATTCATTCAATAAGAAAGCA+TGG 0.542305 tig0016950:-21588 Msa1360180:CDS
ACTTGTAGTAGTCGCAAAAG+AGG 0.542854 tig0016950:-20779 Msa1360180:CDS
TGGTTGACCAAGTACACAAA+GGG 0.543427 tig0016950:-21624 Msa1360180:CDS
TAGTAGGTTGAACTGTTTGA+GGG 0.546289 tig0016950:+20813 None:intergenic
GTGAAGCCCATTCTTGATGA+AGG 0.552695 tig0016950:-21466 Msa1360180:CDS
TTTGTTTGGGATGAAGCCAC+GGG 0.569196 tig0016950:-21439 Msa1360180:CDS
GTAGTAGGTTGAACTGTTTG+AGG 0.569252 tig0016950:+20812 None:intergenic
CTCAGCAGATAGAACAGTCA+AGG 0.570319 tig0016950:-21690 Msa1360180:CDS
TGCAGACGCTATATTAGAGA+TGG 0.575188 tig0016950:-20732 Msa1360180:CDS
GGCATCTGCTTCAAAGATGA+GGG 0.578418 tig0016950:-20711 Msa1360180:CDS
TGGCATCTGCTTCAAAGATG+AGG 0.587021 tig0016950:-20712 Msa1360180:CDS
TAGAACAGTCAAGGGCTAAG+TGG 0.596106 tig0016950:-21681 Msa1360180:CDS
ATTCGCAATTATAGCTCCCG+TGG 0.603592 tig0016950:+21423 None:intergenic
CTTGTAGTAGTCGCAAAAGA+GGG 0.619141 tig0016950:-20778 Msa1360180:CDS
GTGCTCAACAATTCTGGACA+AGG 0.633276 tig0016950:+20887 None:intergenic
AAAACCGACATTCAGTGTTG+AGG 0.637621 tig0016950:-21504 Msa1360180:CDS
TCAGCAGATAGAACAGTCAA+GGG 0.645530 tig0016950:-21689 Msa1360180:CDS
TGAGTCTTCATCTGAATCCG+AGG 0.649200 tig0016950:-20855 Msa1360180:CDS
ATGGTTGACCAAGTACACAA+AGG 0.660204 tig0016950:-21625 Msa1360180:CDS
GTCTCCTCAACACTGAATGT+CGG 0.665231 tig0016950:+21500 None:intergenic
GCCGAATTACAAGATCAAGA+AGG 0.670605 tig0016950:-21720 Msa1360180:CDS
AATTTGGGAAGAATACATCA+AGG 0.671593 tig0016950:-21395 Msa1360180:intron
TGGATACAACAGCACACCTG+CGG 0.685182 tig0016950:+20510 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATGAAAAGATATCTTTT+TGG - tig0016950:21687-21706 Msa1360180:CDS 15.0%
!!! TATATGAAAAGATATCTTTT+TGG - tig0016950:21687-21706 Msa1360180:CDS 15.0%
!! TATGTTAGTTAAACTATAGA+TGG - tig0016950:20411-20430 Msa1360180:three_prime_UTR 20.0%
!! AATAACAACAAAACAAATGA+TGG + tig0016950:20797-20816 None:intergenic 20.0%
!! ATTAATTGAGTACATCAAAA+TGG - tig0016950:20814-20833 Msa1360180:CDS 20.0%
!!! ACTTTTGAATTGTTTCTATT+AGG - tig0016950:20854-20873 Msa1360180:CDS 20.0%
!!! ATATTTTTCAACCTATCTAT+TGG + tig0016950:21670-21689 None:intergenic 20.0%
!! TATGTTAGTTAAACTATAGA+TGG - tig0016950:20411-20430 Msa1360180:three_prime_UTR 20.0%
!! AATAACAACAAAACAAATGA+TGG + tig0016950:20797-20816 None:intergenic 20.0%
!! ATTAATTGAGTACATCAAAA+TGG - tig0016950:20814-20833 Msa1360180:CDS 20.0%
!!! ACTTTTGAATTGTTTCTATT+AGG - tig0016950:20854-20873 Msa1360180:CDS 20.0%
!!! ATATTTTTCAACCTATCTAT+TGG + tig0016950:21670-21689 None:intergenic 20.0%
! ATTCATTCAATAAGAAAGCA+TGG - tig0016950:20569-20588 Msa1360180:CDS 25.0%
! TAATTGCGAATGATGAAATT+TGG - tig0016950:20746-20765 Msa1360180:CDS 25.0%
! AATTGCGAATGATGAAATTT+GGG - tig0016950:20747-20766 Msa1360180:CDS 25.0%
! GTTATTTATCAGAAGATCAA+CGG + tig0016950:21022-21041 None:intergenic 25.0%
! AAGGATTTAGATTTAATGCA+AGG - tig0016950:21519-21538 Msa1360180:CDS 25.0%
! AAAAATATCTCTCTTGCATT+AGG + tig0016950:21591-21610 None:intergenic 25.0%
!!! GATATTTTTGTCTCTCAAAA+AGG - tig0016950:21602-21621 Msa1360180:CDS 25.0%
! AAAAGGATAAACGTTTAACT+TGG - tig0016950:21619-21638 Msa1360180:CDS 25.0%
! ATTCATTCAATAAGAAAGCA+TGG - tig0016950:20569-20588 Msa1360180:CDS 25.0%
! TAATTGCGAATGATGAAATT+TGG - tig0016950:20746-20765 Msa1360180:CDS 25.0%
! AATTGCGAATGATGAAATTT+GGG - tig0016950:20747-20766 Msa1360180:CDS 25.0%
! GTTATTTATCAGAAGATCAA+CGG + tig0016950:21022-21041 None:intergenic 25.0%
! AAGGATTTAGATTTAATGCA+AGG - tig0016950:21519-21538 Msa1360180:CDS 25.0%
! AAAAATATCTCTCTTGCATT+AGG + tig0016950:21591-21610 None:intergenic 25.0%
!!! GATATTTTTGTCTCTCAAAA+AGG - tig0016950:21602-21621 Msa1360180:CDS 25.0%
! AAAAGGATAAACGTTTAACT+TGG - tig0016950:21619-21638 Msa1360180:CDS 25.0%
! GATGGATTTCACAAAAAAAC+AGG - tig0016950:20604-20623 Msa1360180:CDS 30.0%
ACAAAAAAACAGGTCTGAAA+TGG - tig0016950:20614-20633 Msa1360180:CDS 30.0%
! CAAAAAAACAGGTCTGAAAT+GGG - tig0016950:20615-20634 Msa1360180:CDS 30.0%
AATTTGGGAAGAATACATCA+AGG - tig0016950:20762-20781 Msa1360180:CDS 30.0%
! ACAACAAAACAAATGATGGA+TGG + tig0016950:20793-20812 None:intergenic 30.0%
TACAATGGAAAGGAAAGATT+GGG + tig0016950:21052-21071 None:intergenic 30.0%
ATACAATGGAAAGGAAAGAT+TGG + tig0016950:21053-21072 None:intergenic 30.0%
TAGATTGTGCTCAAATACAA+TGG + tig0016950:21067-21086 None:intergenic 30.0%
! TTTAGAGTTATGTTGCTCTA+TGG + tig0016950:21478-21497 None:intergenic 30.0%
! GATGGATTTCACAAAAAAAC+AGG - tig0016950:20604-20623 Msa1360180:CDS 30.0%
ACAAAAAAACAGGTCTGAAA+TGG - tig0016950:20614-20633 Msa1360180:CDS 30.0%
! CAAAAAAACAGGTCTGAAAT+GGG - tig0016950:20615-20634 Msa1360180:CDS 30.0%
AATTTGGGAAGAATACATCA+AGG - tig0016950:20762-20781 Msa1360180:CDS 30.0%
! ACAACAAAACAAATGATGGA+TGG + tig0016950:20793-20812 None:intergenic 30.0%
TACAATGGAAAGGAAAGATT+GGG + tig0016950:21052-21071 None:intergenic 30.0%
ATACAATGGAAAGGAAAGAT+TGG + tig0016950:21053-21072 None:intergenic 30.0%
TAGATTGTGCTCAAATACAA+TGG + tig0016950:21067-21086 None:intergenic 30.0%
! TTTAGAGTTATGTTGCTCTA+TGG + tig0016950:21478-21497 None:intergenic 30.0%
GCCTTCTTGATCTTGTAATT+CGG + tig0016950:20441-20460 None:intergenic 35.0%
TAAGATACTGGCAGATTTGA+TGG - tig0016950:20513-20532 Msa1360180:CDS 35.0%
GTTTATTCCCTTTGTGTACT+TGG + tig0016950:20543-20562 None:intergenic 35.0%
GCATGGAAACATATTTGTGA+TGG - tig0016950:20586-20605 Msa1360180:CDS 35.0%
AAGTCACCTTCATCAAGAAT+GGG + tig0016950:20700-20719 None:intergenic 35.0%
AAAGTCACCTTCATCAAGAA+TGG + tig0016950:20701-20720 None:intergenic 35.0%
TTGATGAAGGTGACTTTGTT+TGG - tig0016950:20704-20723 Msa1360180:CDS 35.0%
! TGATGAAGGTGACTTTGTTT+GGG - tig0016950:20705-20724 Msa1360180:CDS 35.0%
TTTACCCCTCTTCTATAGTA+AGG - tig0016950:20878-20897 Msa1360180:CDS 35.0%
ACAATGGAAAGGAAAGATTG+GGG + tig0016950:21051-21070 None:intergenic 35.0%
TGTGCTCAAATACAATGGAA+AGG + tig0016950:21062-21081 None:intergenic 35.0%
GTACATAGCTCCTTAAAGAT+TGG + tig0016950:21177-21196 None:intergenic 35.0%
! TAGTAGGTTGAACTGTTTGA+GGG + tig0016950:21347-21366 None:intergenic 35.0%
TGCTGTTGTATCCAATAGAT+AGG - tig0016950:21656-21675 Msa1360180:CDS 35.0%
GCCTTCTTGATCTTGTAATT+CGG + tig0016950:20441-20460 None:intergenic 35.0%
TAAGATACTGGCAGATTTGA+TGG - tig0016950:20513-20532 Msa1360180:CDS 35.0%
GTTTATTCCCTTTGTGTACT+TGG + tig0016950:20543-20562 None:intergenic 35.0%
GCATGGAAACATATTTGTGA+TGG - tig0016950:20586-20605 Msa1360180:CDS 35.0%
AAGTCACCTTCATCAAGAAT+GGG + tig0016950:20700-20719 None:intergenic 35.0%
AAAGTCACCTTCATCAAGAA+TGG + tig0016950:20701-20720 None:intergenic 35.0%
TTGATGAAGGTGACTTTGTT+TGG - tig0016950:20704-20723 Msa1360180:CDS 35.0%
! TGATGAAGGTGACTTTGTTT+GGG - tig0016950:20705-20724 Msa1360180:CDS 35.0%
TTTACCCCTCTTCTATAGTA+AGG - tig0016950:20878-20897 Msa1360180:CDS 35.0%
ACAATGGAAAGGAAAGATTG+GGG + tig0016950:21051-21070 None:intergenic 35.0%
TGTGCTCAAATACAATGGAA+AGG + tig0016950:21062-21081 None:intergenic 35.0%
GTACATAGCTCCTTAAAGAT+TGG + tig0016950:21177-21196 None:intergenic 35.0%
! TAGTAGGTTGAACTGTTTGA+GGG + tig0016950:21347-21366 None:intergenic 35.0%
TGCTGTTGTATCCAATAGAT+AGG - tig0016950:21656-21675 Msa1360180:CDS 35.0%
GCCGAATTACAAGATCAAGA+AGG - tig0016950:20437-20456 Msa1360180:three_prime_UTR 40.0%
TCAGCAGATAGAACAGTCAA+GGG - tig0016950:20468-20487 Msa1360180:three_prime_UTR 40.0%
ATGGTTGACCAAGTACACAA+AGG - tig0016950:20532-20551 Msa1360180:CDS 40.0%
TGGTTGACCAAGTACACAAA+GGG - tig0016950:20533-20552 Msa1360180:CDS 40.0%
! AACAGGTCTGAAATGGGATA+AGG - tig0016950:20621-20640 Msa1360180:CDS 40.0%
AAAACCGACATTCAGTGTTG+AGG - tig0016950:20653-20672 Msa1360180:CDS 40.0%
CAAAAGTTTCCTCGTGAAGA+AGG + tig0016950:20841-20860 None:intergenic 40.0%
AGCTCCTTACTATAGAAGAG+GGG + tig0016950:20885-20904 None:intergenic 40.0%
GAGCTCCTTACTATAGAAGA+GGG + tig0016950:20886-20905 None:intergenic 40.0%
AGAGCTCCTTACTATAGAAG+AGG + tig0016950:20887-20906 None:intergenic 40.0%
TCTACCTAAGAGGGAAAAGT+GGG - tig0016950:20904-20923 Msa1360180:CDS 40.0%
TGATGTGTGCTCAACAATTC+TGG + tig0016950:21279-21298 None:intergenic 40.0%
ATTTGTCTCTTCCTCATCCT+CGG + tig0016950:21322-21341 None:intergenic 40.0%
! GTAGTAGGTTGAACTGTTTG+AGG + tig0016950:21348-21367 None:intergenic 40.0%
CAAGTTTCTGTAGGTGTAGT+AGG + tig0016950:21363-21382 None:intergenic 40.0%
CGACTACTACAAGTTTCTGT+AGG + tig0016950:21372-21391 None:intergenic 40.0%
ACTTGTAGTAGTCGCAAAAG+AGG - tig0016950:21378-21397 Msa1360180:intron 40.0%
CTTGTAGTAGTCGCAAAAGA+GGG - tig0016950:21379-21398 Msa1360180:intron 40.0%
TGCAGACGCTATATTAGAGA+TGG - tig0016950:21425-21444 Msa1360180:CDS 40.0%
CAGCATATCTGACTGTGTTA+AGG - tig0016950:21500-21519 Msa1360180:CDS 40.0%
GCCGAATTACAAGATCAAGA+AGG - tig0016950:20437-20456 Msa1360180:three_prime_UTR 40.0%
TCAGCAGATAGAACAGTCAA+GGG - tig0016950:20468-20487 Msa1360180:three_prime_UTR 40.0%
ATGGTTGACCAAGTACACAA+AGG - tig0016950:20532-20551 Msa1360180:CDS 40.0%
TGGTTGACCAAGTACACAAA+GGG - tig0016950:20533-20552 Msa1360180:CDS 40.0%
! AACAGGTCTGAAATGGGATA+AGG - tig0016950:20621-20640 Msa1360180:CDS 40.0%
AAAACCGACATTCAGTGTTG+AGG - tig0016950:20653-20672 Msa1360180:CDS 40.0%
CAAAAGTTTCCTCGTGAAGA+AGG + tig0016950:20841-20860 None:intergenic 40.0%
AGCTCCTTACTATAGAAGAG+GGG + tig0016950:20885-20904 None:intergenic 40.0%
GAGCTCCTTACTATAGAAGA+GGG + tig0016950:20886-20905 None:intergenic 40.0%
AGAGCTCCTTACTATAGAAG+AGG + tig0016950:20887-20906 None:intergenic 40.0%
TCTACCTAAGAGGGAAAAGT+GGG - tig0016950:20904-20923 Msa1360180:CDS 40.0%
TGATGTGTGCTCAACAATTC+TGG + tig0016950:21279-21298 None:intergenic 40.0%
ATTTGTCTCTTCCTCATCCT+CGG + tig0016950:21322-21341 None:intergenic 40.0%
! GTAGTAGGTTGAACTGTTTG+AGG + tig0016950:21348-21367 None:intergenic 40.0%
CAAGTTTCTGTAGGTGTAGT+AGG + tig0016950:21363-21382 None:intergenic 40.0%
CGACTACTACAAGTTTCTGT+AGG + tig0016950:21372-21391 None:intergenic 40.0%
ACTTGTAGTAGTCGCAAAAG+AGG - tig0016950:21378-21397 Msa1360180:intron 40.0%
CTTGTAGTAGTCGCAAAAGA+GGG - tig0016950:21379-21398 Msa1360180:intron 40.0%
TGCAGACGCTATATTAGAGA+TGG - tig0016950:21425-21444 Msa1360180:CDS 40.0%
CAGCATATCTGACTGTGTTA+AGG - tig0016950:21500-21519 Msa1360180:CDS 40.0%
CTCAGCAGATAGAACAGTCA+AGG - tig0016950:20467-20486 Msa1360180:three_prime_UTR 45.0%
TAGAACAGTCAAGGGCTAAG+TGG - tig0016950:20476-20495 Msa1360180:three_prime_UTR 45.0%
AGCATCGCTCACTAAGATAC+TGG - tig0016950:20501-20520 Msa1360180:exon 45.0%
GTCTCCTCAACACTGAATGT+CGG + tig0016950:20660-20679 None:intergenic 45.0%
GTGAAGCCCATTCTTGATGA+AGG - tig0016950:20691-20710 Msa1360180:CDS 45.0%
! CTTTGTTTGGGATGAAGCCA+CGG - tig0016950:20717-20736 Msa1360180:CDS 45.0%
! TTTGTTTGGGATGAAGCCAC+GGG - tig0016950:20718-20737 Msa1360180:CDS 45.0%
ATTCGCAATTATAGCTCCCG+TGG + tig0016950:20737-20756 None:intergenic 45.0%
CAAAATGGTCCTTCTTCACG+AGG - tig0016950:20829-20848 Msa1360180:CDS 45.0%
AGTAAGGAGCTCTACCTAAG+AGG - tig0016950:20894-20913 Msa1360180:CDS 45.0%
GTAAGGAGCTCTACCTAAGA+GGG - tig0016950:20895-20914 Msa1360180:CDS 45.0%
CTCTACCTAAGAGGGAAAAG+TGG - tig0016950:20903-20922 Msa1360180:CDS 45.0%
CTACCTAAGAGGGAAAAGTG+GGG - tig0016950:20905-20924 Msa1360180:CDS 45.0%
!!! GCCACCACTTTTTACAGTCT+CGG + tig0016950:21151-21170 None:intergenic 45.0%
TGGTGGCTGTCCAATCTTTA+AGG - tig0016950:21164-21183 Msa1360180:intron 45.0%
AATTCTGGACAAGGAGCTCT+AGG + tig0016950:21264-21283 None:intergenic 45.0%
GTGCTCAACAATTCTGGACA+AGG + tig0016950:21273-21292 None:intergenic 45.0%
TGAGTCTTCATCTGAATCCG+AGG - tig0016950:21302-21321 Msa1360180:intron 45.0%
TTCATCTGAATCCGAGGATG+AGG - tig0016950:21308-21327 Msa1360180:intron 45.0%
TGGCATCTGCTTCAAAGATG+AGG - tig0016950:21445-21464 Msa1360180:CDS 45.0%
GGCATCTGCTTCAAAGATGA+GGG - tig0016950:21446-21465 Msa1360180:CDS 45.0%
CTCAGCAGATAGAACAGTCA+AGG - tig0016950:20467-20486 Msa1360180:three_prime_UTR 45.0%
TAGAACAGTCAAGGGCTAAG+TGG - tig0016950:20476-20495 Msa1360180:three_prime_UTR 45.0%
AGCATCGCTCACTAAGATAC+TGG - tig0016950:20501-20520 Msa1360180:exon 45.0%
GTCTCCTCAACACTGAATGT+CGG + tig0016950:20660-20679 None:intergenic 45.0%
GTGAAGCCCATTCTTGATGA+AGG - tig0016950:20691-20710 Msa1360180:CDS 45.0%
! CTTTGTTTGGGATGAAGCCA+CGG - tig0016950:20717-20736 Msa1360180:CDS 45.0%
! TTTGTTTGGGATGAAGCCAC+GGG - tig0016950:20718-20737 Msa1360180:CDS 45.0%
ATTCGCAATTATAGCTCCCG+TGG + tig0016950:20737-20756 None:intergenic 45.0%
CAAAATGGTCCTTCTTCACG+AGG - tig0016950:20829-20848 Msa1360180:CDS 45.0%
AGTAAGGAGCTCTACCTAAG+AGG - tig0016950:20894-20913 Msa1360180:CDS 45.0%
GTAAGGAGCTCTACCTAAGA+GGG - tig0016950:20895-20914 Msa1360180:CDS 45.0%
CTCTACCTAAGAGGGAAAAG+TGG - tig0016950:20903-20922 Msa1360180:CDS 45.0%
CTACCTAAGAGGGAAAAGTG+GGG - tig0016950:20905-20924 Msa1360180:CDS 45.0%
!!! GCCACCACTTTTTACAGTCT+CGG + tig0016950:21151-21170 None:intergenic 45.0%
TGGTGGCTGTCCAATCTTTA+AGG - tig0016950:21164-21183 Msa1360180:intron 45.0%
AATTCTGGACAAGGAGCTCT+AGG + tig0016950:21264-21283 None:intergenic 45.0%
GTGCTCAACAATTCTGGACA+AGG + tig0016950:21273-21292 None:intergenic 45.0%
TGAGTCTTCATCTGAATCCG+AGG - tig0016950:21302-21321 Msa1360180:intron 45.0%
TTCATCTGAATCCGAGGATG+AGG - tig0016950:21308-21327 Msa1360180:intron 45.0%
TGGCATCTGCTTCAAAGATG+AGG - tig0016950:21445-21464 Msa1360180:CDS 45.0%
GGCATCTGCTTCAAAGATGA+GGG - tig0016950:21446-21465 Msa1360180:CDS 45.0%
!! ACTCCCCACTTTTCCCTCTT+AGG + tig0016950:20911-20930 None:intergenic 50.0%
! CTTTTTACAGTCTCGGCGTC+AGG + tig0016950:21144-21163 None:intergenic 50.0%
GACGCCGAGACTGTAAAAAG+TGG - tig0016950:21144-21163 Msa1360180:intron 50.0%
GCCGAGACTGTAAAAAGTGG+TGG - tig0016950:21147-21166 Msa1360180:intron 50.0%
TTCTCTGACGCAGCAACGAA+CGG - tig0016950:21201-21220 Msa1360180:intron 50.0%
! AGTCGCAAAAGAGGGCGTAA+AGG - tig0016950:21387-21406 Msa1360180:intron 50.0%
! AGAGGGCGTAAAGGAGTTGA+TGG - tig0016950:21396-21415 Msa1360180:CDS 50.0%
!! GTTTAACTTGGTTGCACCGC+AGG - tig0016950:21631-21650 Msa1360180:CDS 50.0%
TGGATACAACAGCACACCTG+CGG + tig0016950:21650-21669 None:intergenic 50.0%
!! ACTCCCCACTTTTCCCTCTT+AGG + tig0016950:20911-20930 None:intergenic 50.0%
! CTTTTTACAGTCTCGGCGTC+AGG + tig0016950:21144-21163 None:intergenic 50.0%
GACGCCGAGACTGTAAAAAG+TGG - tig0016950:21144-21163 Msa1360180:intron 50.0%
GCCGAGACTGTAAAAAGTGG+TGG - tig0016950:21147-21166 Msa1360180:intron 50.0%
TTCTCTGACGCAGCAACGAA+CGG - tig0016950:21201-21220 Msa1360180:intron 50.0%
! AGTCGCAAAAGAGGGCGTAA+AGG - tig0016950:21387-21406 Msa1360180:intron 50.0%
! AGAGGGCGTAAAGGAGTTGA+TGG - tig0016950:21396-21415 Msa1360180:CDS 50.0%
!! GTTTAACTTGGTTGCACCGC+AGG - tig0016950:21631-21650 Msa1360180:CDS 50.0%
TGGATACAACAGCACACCTG+CGG + tig0016950:21650-21669 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
tig0016950 gene 20396 21783 20396 ID=Msa1360180;Name=Msa1360180
tig0016950 mRNA 20396 21783 20396 ID=Msa1360180-mRNA-1;Parent=Msa1360180;Name=Msa1360180-mRNA-1;_AED=0.02;_eAED=0.02;_QI=34|1|1|1|1|1|2|106|298
tig0016950 exon 20396 21044 20396 ID=Msa1360180-mRNA-1:exon:11801;Parent=Msa1360180-mRNA-1
tig0016950 exon 21396 21783 21396 ID=Msa1360180-mRNA-1:exon:11800;Parent=Msa1360180-mRNA-1
tig0016950 five_prime_UTR 21750 21783 21750 ID=Msa1360180-mRNA-1:five_prime_utr;Parent=Msa1360180-mRNA-1
tig0016950 CDS 21396 21749 21396 ID=Msa1360180-mRNA-1:cds;Parent=Msa1360180-mRNA-1
tig0016950 CDS 20502 21044 20502 ID=Msa1360180-mRNA-1:cds;Parent=Msa1360180-mRNA-1
tig0016950 three_prime_UTR 20396 20501 20396 ID=Msa1360180-mRNA-1:three_prime_utr;Parent=Msa1360180-mRNA-1
Gene Sequence

>Msa1360180

ATGGCACGCCGAATTACAAGATCAAGAAGGCTAGAAACTCAGCAGATAGAACAGTCAAGGGCTAAGTGGACAGCATCGCTCACTAAGATACTGGCAGATTTGATGGTTGACCAAGTACACAAAGGGAATAAACAAAACAATTCATTCAATAAGAAAGCATGGAAACATATTTGTGATGGATTTCACAAAAAAACAGGTCTGAAATGGGATAAGGAACAACTCAAAAACCGACATTCAGTGTTGAGGAGACAGTATGCTATTGTGAAGCCCATTCTTGATGAAGGTGACTTTGTTTGGGATGAAGCCACGGGAGCTATAATTGCGAATGATGAAATTTGGGAAGAATACATCAAGAACAATCCTGACGCCGAGACTGTAAAAAGTGGTGGCTGTCCAATCTTTAAGGAGCTATGTACAATATTCTCTGACGCAGCAACGAACGGAAAACATGAATATGCTGCATCTGATAGTGAACACACTCCTAGAGCTCCTTGTCCAGAATTGTTGAGCACACATCAAGATGAGTCTTCATCTGAATCCGAGGATGAGGAAGAGACAAATGACCCTCAAACAGTTCAACCTACTACACCTACAGAAACTTGTAGTAGTCGCAAAAGAGGGCGTAAAGGAGTTGATGGTGCTATTGCAGACGCTATATTAGAGATGGCATCTGCTTCAAAGATGAGGGCAGCTGCCATAGAGCAACATAACTCTAAATACAGCATATCTGACTGTGTTAAGGATTTAGATTTAATGCAAGGTGTTGATCAACAGCTATACTTTGCTGCTCTAGATCTTTTCAACAATCCTAATGCAAGAGAGATATTTTTGTCTCTCAAAAAGGATAAACGTTTAACTTGGTTGCACCGCAGGTGTGCTGTTGTATCCAATAGATAG
Protein sequence

>Msa1360180

MARRITRSRRLETQQIEQSRAKWTASLTKILADLMVDQVHKGNKQNNSFNKKAWKHICDGFHKKTGLKWDKEQLKNRHSVLRRQYAIVKPILDEGDFVWDEATGAIIANDEIWEEYIKNNPDAETVKSGGCPIFKELCTIFSDAATNGKHEYAASDSEHTPRAPCPELLSTHQDESSSESEDEEETNDPQTVQPTTPTETCSSRKRGRKGVDGAIADAILEMASASKMRAAAIEQHNSKYSISDCVKDLDLMQGVDQQLYFAALDLFNNPNAREIFLSLKKDKRLTWLHRRCAVVSNR*