AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1465680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465680 MtrunA17_Chr7g0256551 96.575 146 4 1 12 156 94 239 2.05e-96 278
Msa1465680 MtrunA17_Chr8g0352871 73.016 63 17 0 12 74 93 155 1.26e-28 103
Msa1465680 MtrunA17_Chr3g0082861 79.661 59 12 0 12 70 89 147 1.63e-28 103
Msa1465680 MtrunA17_Chr5g0414931 52.857 70 33 0 12 81 96 165 1.34e-20 84.7
Msa1465680 MtrunA17_Chr4g0059571 52.778 72 34 0 12 83 102 173 1.81e-20 83.6
Msa1465680 MtrunA17_Chr3g0124861 64.151 53 19 0 14 66 117 169 7.62e-19 79.7
Msa1465680 MtrunA17_Chr3g0118211 50.746 67 33 0 12 78 99 165 7.06e-18 76.6
Msa1465680 MtrunA17_Chr1g0170741 61.538 52 20 0 12 63 87 138 8.30e-18 76.3
Msa1465680 MtrunA17_Chr3g0128691 59.091 44 18 0 16 59 100 143 6.81e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465680 AT3G57040.2 55.034 149 54 5 12 156 95 234 3.53e-43 143
Msa1465680 AT3G57040.1 55.034 149 54 5 12 156 95 234 3.53e-43 143
Msa1465680 AT2G41310.1 51.034 145 59 4 12 156 93 225 2.27e-37 128
Msa1465680 AT2G07440.1 48.951 143 58 5 14 156 9 136 4.53e-34 117
Msa1465680 AT1G10470.2 59.677 62 24 1 13 73 35 96 5.82e-19 79.3
Msa1465680 AT1G10470.3 57.143 63 26 1 12 73 96 158 1.32e-18 79.7
Msa1465680 AT1G10470.1 57.143 63 26 1 12 73 110 172 1.39e-18 80.1
Msa1465680 AT3G48100.1 60.000 55 22 0 12 66 102 156 2.71e-18 77.8
Msa1465680 AT1G59940.1 57.407 54 23 0 12 65 109 162 3.35e-18 78.6
Msa1465680 AT5G62920.1 60.000 55 22 0 12 66 101 155 3.49e-18 77.4
Msa1465680 AT2G40670.2 58.182 55 23 0 13 67 110 164 6.27e-18 76.3
Msa1465680 AT2G40670.1 53.226 62 27 1 8 67 102 163 1.85e-17 75.1
Msa1465680 AT1G59940.2 57.407 54 23 0 12 65 201 254 2.71e-17 77.4
Msa1465680 AT3G56380.2 60.784 51 20 0 13 63 99 149 5.22e-17 73.6
Msa1465680 AT3G56380.1 57.692 52 22 0 12 63 97 148 1.61e-16 72.4
Msa1465680 AT1G74890.1 65.306 49 17 0 12 60 94 142 2.92e-16 72.8
Msa1465680 AT1G19050.1 61.224 49 19 0 12 60 100 148 1.05e-15 71.2

Find 30 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAAATCTGATAGCCTTAC+AGG 0.185020 tig0045407:-456 Msa1465680:intergenic
ACAATGAAGCACCGAGATTT+TGG 0.221714 tig0045407:-1095 Msa1465680:intergenic
TTGATTTGGATCTTTCAATT+TGG 0.240297 tig0045407:-505 Msa1465680:intergenic
GAGAGAATCTTATAAGTCTT+TGG 0.270519 tig0045407:-208 Msa1465680:intergenic
AAGATTCTCTCTGATATTAC+AGG 0.275607 tig0045407:+220 Msa1465680:CDS
ACTCTTTCTCTTGTTATTGT+TGG 0.284458 tig0045407:-685 Msa1465680:intergenic
TATTTCCAGGAAATGTTTAC+AGG 0.304991 tig0045407:-911 Msa1465680:intergenic
TGTTCCCTGTAAACATTTCC+TGG 0.307533 tig0045407:+906 Msa1465680:three_prime_UTR
TCTTATAAGTCTTTGGTTCC+AGG 0.309416 tig0045407:-201 Msa1465680:intergenic
ACTTGGGTGTTGGTTTATAT+AGG 0.347253 tig0045407:+859 Msa1465680:three_prime_UTR
AATTGGAAGGGGATATTTCC+AGG 0.353288 tig0045407:-924 Msa1465680:intergenic
ATGTGCCTTCAAGAATTAAT+AGG 0.368999 tig0045407:+291 Msa1465680:CDS
TATTACCTATTAATTCTTGA+AGG 0.405802 tig0045407:-296 Msa1465680:intergenic
TTGGTTTATATAGGCTCTAT+AGG 0.429787 tig0045407:+868 Msa1465680:three_prime_UTR
TTAAATATTAACTTGGGTGT+TGG 0.433869 tig0045407:+849 Msa1465680:three_prime_UTR
TTGTATGATGATAAATCATT+TGG 0.463555 tig0045407:+772 Msa1465680:exon
GTGGCTATGCCACTGTATCT+TGG 0.467016 tig0045407:-747 Msa1465680:intergenic
TATCAAATGTAACAGATGCT+TGG 0.497655 tig0045407:+404 Msa1465680:intron
TCATATGAAACAAAATTGGA+AGG 0.508230 tig0045407:-937 Msa1465680:intergenic
CATATGAAACAAAATTGGAA+GGG 0.522607 tig0045407:-936 Msa1465680:intergenic
CAAATGTAACAGATGCTTGG+AGG 0.523574 tig0045407:+407 Msa1465680:intron
ATATGAAACAAAATTGGAAG+GGG 0.538359 tig0045407:-935 Msa1465680:intergenic
TCAGATGACATAATCACCAC+TGG 0.584254 tig0045407:-266 Msa1465680:intergenic
TGTAACAGATGCTTGGAGGA+AGG 0.588489 tig0045407:+411 Msa1465680:intron
ATTTCCAGGAAATGTTTACA+GGG 0.615752 tig0045407:-910 Msa1465680:intergenic
AAATTGAATGTGTAAGAGCA+TGG 0.635971 tig0045407:+975 Msa1465680:three_prime_UTR
CAATAACAAGAGAAAGAGTG+TGG 0.644460 tig0045407:+689 Msa1465680:CDS
ATCATTGAGAAACATACCAG+TGG 0.696105 tig0045407:+250 Msa1465680:CDS
GATTTATCATCATACAACAG+TGG 0.696351 tig0045407:-766 Msa1465680:intergenic
AGAACAAGACCAAGATACAG+TGG 0.701894 tig0045407:+738 Msa1465680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGAAATAAAATGAAATA+AGG - tig0045407:334-353 Msa1465680:intergenic 10.0%
!! AATAGAAATAAAATGAAATA+AGG - tig0045407:334-353 Msa1465680:intergenic 10.0%
!!! ATTTTCATATGAAACAAAAT+TGG - tig0045407:944-963 Msa1465680:intergenic 15.0%
!!! ATTTTCATATGAAACAAAAT+TGG - tig0045407:944-963 Msa1465680:intergenic 15.0%
!! TATTACCTATTAATTCTTGA+AGG - tig0045407:299-318 Msa1465680:intergenic 20.0%
!!! TTATTTCTATTTTTCCACTT+TGG + tig0045407:343-362 Msa1465680:intron 20.0%
!! TATAATTAAAATGACCAAAG+TGG - tig0045407:360-379 Msa1465680:intergenic 20.0%
!! TTGTATGATGATAAATCATT+TGG + tig0045407:772-791 Msa1465680:exon 20.0%
!! TATTACCTATTAATTCTTGA+AGG - tig0045407:299-318 Msa1465680:intergenic 20.0%
!!! TTATTTCTATTTTTCCACTT+TGG + tig0045407:343-362 Msa1465680:intron 20.0%
!! TATAATTAAAATGACCAAAG+TGG - tig0045407:360-379 Msa1465680:intergenic 20.0%
!! TTGTATGATGATAAATCATT+TGG + tig0045407:772-791 Msa1465680:exon 20.0%
!!! TTGATTTGGATCTTTCAATT+TGG - tig0045407:508-527 Msa1465680:intergenic 25.0%
!!! TTTTGTTCTGTTTCTTGATT+TGG - tig0045407:522-541 Msa1465680:intergenic 25.0%
!! TTAAATATTAACTTGGGTGT+TGG + tig0045407:849-868 Msa1465680:three_prime_UTR 25.0%
! ATATGAAACAAAATTGGAAG+GGG - tig0045407:938-957 Msa1465680:intergenic 25.0%
! CATATGAAACAAAATTGGAA+GGG - tig0045407:939-958 Msa1465680:intergenic 25.0%
! TCATATGAAACAAAATTGGA+AGG - tig0045407:940-959 Msa1465680:intergenic 25.0%
!!! TTGATTTGGATCTTTCAATT+TGG - tig0045407:508-527 Msa1465680:intergenic 25.0%
!!! TTTTGTTCTGTTTCTTGATT+TGG - tig0045407:522-541 Msa1465680:intergenic 25.0%
!! TTAAATATTAACTTGGGTGT+TGG + tig0045407:849-868 Msa1465680:three_prime_UTR 25.0%
! ATATGAAACAAAATTGGAAG+GGG - tig0045407:938-957 Msa1465680:intergenic 25.0%
! CATATGAAACAAAATTGGAA+GGG - tig0045407:939-958 Msa1465680:intergenic 25.0%
! TCATATGAAACAAAATTGGA+AGG - tig0045407:940-959 Msa1465680:intergenic 25.0%
GAGAGAATCTTATAAGTCTT+TGG - tig0045407:211-230 None:intergenic 30.0%
AAGATTCTCTCTGATATTAC+AGG + tig0045407:220-239 Msa1465680:CDS 30.0%
ATGTGCCTTCAAGAATTAAT+AGG + tig0045407:291-310 Msa1465680:CDS 30.0%
TATCAAATGTAACAGATGCT+TGG + tig0045407:404-423 Msa1465680:intron 30.0%
! TTTAAATCTGATAGCCTTAC+AGG - tig0045407:459-478 Msa1465680:intergenic 30.0%
!!! AATTTGGTTTTCTTCATGTG+AGG - tig0045407:492-511 Msa1465680:intergenic 30.0%
!! ACTCTTTCTCTTGTTATTGT+TGG - tig0045407:688-707 Msa1465680:intergenic 30.0%
GATTTATCATCATACAACAG+TGG - tig0045407:769-788 Msa1465680:intergenic 30.0%
TTGGTTTATATAGGCTCTAT+AGG + tig0045407:868-887 Msa1465680:three_prime_UTR 30.0%
ATTTCCAGGAAATGTTTACA+GGG - tig0045407:913-932 Msa1465680:intergenic 30.0%
TATTTCCAGGAAATGTTTAC+AGG - tig0045407:914-933 Msa1465680:intergenic 30.0%
AAATTGAATGTGTAAGAGCA+TGG + tig0045407:975-994 Msa1465680:three_prime_UTR 30.0%
GAGAGAATCTTATAAGTCTT+TGG - tig0045407:211-230 Msa1465680:intergenic 30.0%
AAGATTCTCTCTGATATTAC+AGG + tig0045407:220-239 Msa1465680:CDS 30.0%
ATGTGCCTTCAAGAATTAAT+AGG + tig0045407:291-310 Msa1465680:CDS 30.0%
TATCAAATGTAACAGATGCT+TGG + tig0045407:404-423 Msa1465680:intron 30.0%
! TTTAAATCTGATAGCCTTAC+AGG - tig0045407:459-478 Msa1465680:intergenic 30.0%
!!! AATTTGGTTTTCTTCATGTG+AGG - tig0045407:492-511 Msa1465680:intergenic 30.0%
!! ACTCTTTCTCTTGTTATTGT+TGG - tig0045407:688-707 Msa1465680:intergenic 30.0%
GATTTATCATCATACAACAG+TGG - tig0045407:769-788 Msa1465680:intergenic 30.0%
TTGGTTTATATAGGCTCTAT+AGG + tig0045407:868-887 Msa1465680:three_prime_UTR 30.0%
ATTTCCAGGAAATGTTTACA+GGG - tig0045407:913-932 Msa1465680:intergenic 30.0%
TATTTCCAGGAAATGTTTAC+AGG - tig0045407:914-933 Msa1465680:intergenic 30.0%
AAATTGAATGTGTAAGAGCA+TGG + tig0045407:975-994 Msa1465680:three_prime_UTR 30.0%
ATCATTGAGAAACATACCAG+TGG + tig0045407:250-269 Msa1465680:CDS 35.0%
! AGTTTTTCTTGAAGCCTGTA+AGG + tig0045407:442-461 Msa1465680:CDS 35.0%
CAATAACAAGAGAAAGAGTG+TGG + tig0045407:689-708 Msa1465680:CDS 35.0%
!! ACTTGGGTGTTGGTTTATAT+AGG + tig0045407:859-878 Msa1465680:three_prime_UTR 35.0%
! TGTAGCTTTTGCCAAAATCT+CGG + tig0045407:1084-1103 Msa1465680:three_prime_UTR 35.0%
ATCATTGAGAAACATACCAG+TGG + tig0045407:250-269 Msa1465680:CDS 35.0%
! AGTTTTTCTTGAAGCCTGTA+AGG + tig0045407:442-461 Msa1465680:CDS 35.0%
CAATAACAAGAGAAAGAGTG+TGG + tig0045407:689-708 Msa1465680:CDS 35.0%
!! ACTTGGGTGTTGGTTTATAT+AGG + tig0045407:859-878 Msa1465680:three_prime_UTR 35.0%
! TGTAGCTTTTGCCAAAATCT+CGG + tig0045407:1084-1103 Msa1465680:three_prime_UTR 35.0%
TCAGATGACATAATCACCAC+TGG - tig0045407:269-288 Msa1465680:intergenic 40.0%
CAAATGTAACAGATGCTTGG+AGG + tig0045407:407-426 Msa1465680:intron 40.0%
AGAACAAGACCAAGATACAG+TGG + tig0045407:738-757 Msa1465680:CDS 40.0%
TGTTCCCTGTAAACATTTCC+TGG + tig0045407:906-925 Msa1465680:three_prime_UTR 40.0%
AATTGGAAGGGGATATTTCC+AGG - tig0045407:927-946 Msa1465680:intergenic 40.0%
TCAGATGACATAATCACCAC+TGG - tig0045407:269-288 Msa1465680:intergenic 40.0%
CAAATGTAACAGATGCTTGG+AGG + tig0045407:407-426 Msa1465680:intron 40.0%
AGAACAAGACCAAGATACAG+TGG + tig0045407:738-757 Msa1465680:CDS 40.0%
TGTTCCCTGTAAACATTTCC+TGG + tig0045407:906-925 Msa1465680:three_prime_UTR 40.0%
AATTGGAAGGGGATATTTCC+AGG - tig0045407:927-946 Msa1465680:intergenic 40.0%
TGTAACAGATGCTTGGAGGA+AGG + tig0045407:411-430 Msa1465680:intron 45.0%
TGTAACAGATGCTTGGAGGA+AGG + tig0045407:411-430 Msa1465680:intron 45.0%
!! TTAAAAATTAAATATTAACT+TGG + tig0045407:842-861 Msa1465680:three_prime_UTR 5.0%
!! TAAAAATTAAATATTAACTT+GGG + tig0045407:843-862 Msa1465680:three_prime_UTR 5.0%
!! TTAAAAATTAAATATTAACT+TGG + tig0045407:842-861 Msa1465680:three_prime_UTR 5.0%
!! TAAAAATTAAATATTAACTT+GGG + tig0045407:843-862 Msa1465680:three_prime_UTR 5.0%
GTGGCTATGCCACTGTATCT+TGG - tig0045407:750-769 Msa1465680:intergenic 50.0%
!! TGCCAAAATCTCGGTGCTTC+TGG + tig0045407:1093-1112 Msa1465680:three_prime_UTR 50.0%
GTGGCTATGCCACTGTATCT+TGG - tig0045407:750-769 Msa1465680:intergenic 50.0%
Chromosome Type Strat End Strand Name
tig0045407 gene 203 1112 203 ID=Msa1465680;Name=Msa1465680
tig0045407 mRNA 203 1112 203 ID=Msa1465680-mRNA-1;Parent=Msa1465680;Name=Msa1465680-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|333|156
tig0045407 exon 203 312 203 ID=Msa1465680-mRNA-1:exon:24853;Parent=Msa1465680-mRNA-1
tig0045407 exon 419 1112 419 ID=Msa1465680-mRNA-1:exon:24854;Parent=Msa1465680-mRNA-1
tig0045407 CDS 203 312 203 ID=Msa1465680-mRNA-1:cds;Parent=Msa1465680-mRNA-1
tig0045407 CDS 419 779 419 ID=Msa1465680-mRNA-1:cds;Parent=Msa1465680-mRNA-1
tig0045407 three_prime_UTR 780 1112 780 ID=Msa1465680-mRNA-1:three_prime_utr;Parent=Msa1465680-mRNA-1
Gene Sequence

>Msa1465680

TGGAACCAAAGACTTATAAGATTCTCTCTGATATTACAGGAATCATCATCATTGAGAAACATACCAGTGGTGATTATGTCATCTGAAAATGTGCCTTCAAGAATTAATAGATGCTTGGAGGAAGGAGCAGAAGAGTTTTTCTTGAAGCCTGTAAGGCTATCAGATTTAAACAAACTTAAACCTCACATGAAGAAAACCAAATTGAAAGATCCAAATCAAGAAACAGAACAAAAGATAGAAAATTCAGAAATTCTTGAAAATGAACAGCATCAAGCATCAAAATCTCAACATTCACATTTAGAGTCAGAGTCACAAACACAGACACAGTTACAAGCACATTCAACAATAGATCAACAGCAACAATCACTACAACAAGCCAACAATAACAAGAGAAAGAGTGTGGAACAAAGTCTTTCATCTGAAACTGACAGAACAAGACCAAGATACAGTGGCATAGCCACTGTTGTATGA

Protein sequence

>Msa1465680

WNQRLIRFSLILQESSSLRNIPVVIMSSENVPSRINRCLEEGAEEFFLKPVRLSDLNKLKPHMKKTKLKDPNQETEQKIENSEILENEQHQASKSQHSHLESESQTQTQLQAHSTIDQQQQSLQQANNNKRKSVEQSLSSETDRTRPRYSGIATVV*