Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0800270 | RHN54350.1 | 95.302 | 298 | 14 | 0 | 1 | 298 | 31 | 328 | 0.0 | 576 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0800270 | sp|Q9FFJ8|LIMYB_ARATH | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.15e-27 | 113 |
| Msa0800270 | sp|Q9FFJ8|LIMYB_ARATH | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.81e-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0800270 | A0A396HLV9 | 95.302 | 298 | 14 | 0 | 1 | 298 | 31 | 328 | 0.0 | 576 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030280 | Msa0800270 | 0.804894 | 1.735307e-49 | -8.615850e-47 |
| Msa0033110 | Msa0800270 | 0.800993 | 1.110669e-48 | -8.615850e-47 |
| Msa0040690 | Msa0800270 | 0.808491 | 3.017177e-50 | -8.615850e-47 |
| Msa0052610 | Msa0800270 | 0.824982 | 6.009250e-54 | -8.615850e-47 |
| Msa0067870 | Msa0800270 | 0.814165 | 1.769849e-51 | -8.615850e-47 |
| Msa0077880 | Msa0800270 | 0.830958 | 2.189962e-55 | -8.615850e-47 |
| Msa0079110 | Msa0800270 | 0.803217 | 3.874273e-49 | -8.615850e-47 |
| Msa0097850 | Msa0800270 | 0.827196 | 1.787587e-54 | -8.615850e-47 |
| Msa0615440 | Msa0800270 | 0.811653 | 6.287741e-51 | -8.615850e-47 |
| Msa0641140 | Msa0800270 | 0.805579 | 1.247269e-49 | -8.615850e-47 |
| Msa0691900 | Msa0800270 | 0.815314 | 9.848304e-52 | -8.615850e-47 |
| Msa0698490 | Msa0800270 | 0.810716 | 1.003522e-50 | -8.615850e-47 |
| Msa0698660 | Msa0800270 | 0.809899 | 1.506434e-50 | -8.615850e-47 |
| Msa0708520 | Msa0800270 | 0.809412 | 1.916357e-50 | -8.615850e-47 |
| Msa0784380 | Msa0800270 | 0.802933 | 4.434688e-49 | -8.615850e-47 |
| Msa0800270 | Msa0838260 | 0.976162 | 2.661598e-141 | -8.615850e-47 |
| Msa0800270 | Msa0906780 | 0.812087 | 5.056308e-51 | -8.615850e-47 |
| Msa0800270 | Msa0962840 | 0.803664 | 3.130063e-49 | -8.615850e-47 |
| Msa0800270 | Msa1006840 | 0.818633 | 1.769961e-52 | -8.615850e-47 |
| Msa0800270 | Msa1041520 | 0.803876 | 2.828005e-49 | -8.615850e-47 |
| Msa0800270 | Msa1079230 | 0.819103 | 1.384327e-52 | -8.615850e-47 |
| Msa0800270 | Msa1080640 | 0.819473 | 1.140438e-52 | -8.615850e-47 |
| Msa0800270 | Msa1080800 | 0.816397 | 5.648495e-52 | -8.615850e-47 |
| Msa0800270 | Msa1118940 | 0.818255 | 2.155900e-52 | -8.615850e-47 |
| Msa0800270 | Msa1135890 | 0.813966 | 1.958317e-51 | -8.615850e-47 |
| Msa0800270 | Msa1163540 | 0.804219 | 2.399952e-49 | -8.615850e-47 |
| Msa0800270 | Msa1163650 | -0.811015 | 8.647434e-51 | -8.615850e-47 |
| Msa0800270 | Msa1256270 | -0.801168 | 1.022760e-48 | -8.615850e-47 |
| Msa0800270 | Msa1307600 | 0.809106 | 2.228707e-50 | -8.615850e-47 |
| Msa0800270 | Msa1360180 | 0.970538 | 9.062266e-132 | -8.615850e-47 |
| Msa0800270 | Msa1360980 | 0.843851 | 1.086407e-58 | -8.615850e-47 |
| Msa0800270 | Msa1370620 | 0.818468 | 1.929280e-52 | -8.615850e-47 |
| Msa0800270 | Msa1376090 | -0.803857 | 2.854023e-49 | -8.615850e-47 |
| Msa0800270 | Msa1386710 | 0.805740 | 1.153886e-49 | -8.615850e-47 |
| Msa0800270 | Msa1416370 | 0.819300 | 1.248531e-52 | -8.615850e-47 |
| Msa0800270 | Msa1423350 | 0.824733 | 6.879861e-54 | -8.615850e-47 |
| Msa0800270 | Msa1444310 | 0.814122 | 1.808941e-51 | -8.615850e-47 |
| Msa0800270 | Msa1445390 | -0.825473 | 4.598559e-54 | -8.615850e-47 |
| Msa0800270 | Msa1449340 | -0.827698 | 1.354741e-54 | -8.615850e-47 |
| Msa0800270 | Msa1465680 | 0.834720 | 2.546073e-56 | -8.615850e-47 |
| Msa0125770 | Msa0800270 | 0.838956 | 2.112382e-57 | -8.615850e-47 |
| Msa0131330 | Msa0800270 | 0.818693 | 1.715500e-52 | -8.615850e-47 |
| Msa0140500 | Msa0800270 | -0.823533 | 1.316294e-53 | -8.615850e-47 |
| Msa0150990 | Msa0800270 | 0.806938 | 6.451912e-50 | -8.615850e-47 |
| Msa0170410 | Msa0800270 | 0.823143 | 1.623540e-53 | -8.615850e-47 |
| Msa0189300 | Msa0800270 | 0.803474 | 3.427084e-49 | -8.615850e-47 |
| Msa0209500 | Msa0800270 | 0.805373 | 1.377740e-49 | -8.615850e-47 |
| Msa0227400 | Msa0800270 | 0.807061 | 6.074864e-50 | -8.615850e-47 |
| Msa0365860 | Msa0800270 | 0.811366 | 7.256919e-51 | -8.615850e-47 |
| Msa0381610 | Msa0800270 | -0.800815 | 1.207464e-48 | -8.615850e-47 |
| Msa0277450 | Msa0800270 | 0.804316 | 2.290897e-49 | -8.615850e-47 |
| Msa0278790 | Msa0800270 | 0.813040 | 3.130549e-51 | -8.615850e-47 |
| Msa0281430 | Msa0800270 | 0.812382 | 4.360019e-51 | -8.615850e-47 |
| Msa0292120 | Msa0800270 | 0.807290 | 5.432417e-50 | -8.615850e-47 |
| Msa0357900 | Msa0800270 | 0.800233 | 1.586804e-48 | -8.615850e-47 |
| Msa0357990 | Msa0800270 | 0.802850 | 4.613202e-49 | -8.615850e-47 |
| Msa0363680 | Msa0800270 | 0.813172 | 2.927798e-51 | -8.615850e-47 |
| Msa0545060 | Msa0800270 | 0.808418 | 3.127265e-50 | -8.615850e-47 |
| Msa0600600 | Msa0800270 | 0.808059 | 3.730212e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0800270 | MtrunA17_Chr5g0405761 | 95.302 | 298 | 14 | 0 | 1 | 298 | 31 | 328 | 0.0 | 576 |
| Msa0800270 | MtrunA17_Chr1g0156481 | 40.333 | 300 | 153 | 9 | 17 | 298 | 12 | 303 | 8.24e-62 | 198 |
| Msa0800270 | MtrunA17_Chr1g0176881 | 31.915 | 188 | 112 | 3 | 16 | 200 | 18 | 192 | 1.22e-28 | 115 |
| Msa0800270 | MtrunA17_Chr1g0176881 | 32.031 | 128 | 87 | 0 | 18 | 145 | 191 | 318 | 2.25e-22 | 97.1 |
| Msa0800270 | MtrunA17_Chr3g0113081 | 28.475 | 295 | 181 | 6 | 3 | 294 | 366 | 633 | 6.79e-28 | 113 |
| Msa0800270 | MtrunA17_Chr3g0113081 | 30.047 | 213 | 135 | 4 | 3 | 205 | 164 | 372 | 5.23e-24 | 102 |
| Msa0800270 | MtrunA17_Chr3g0113081 | 34.959 | 123 | 80 | 0 | 19 | 141 | 7 | 129 | 5.39e-18 | 84.3 |
| Msa0800270 | MtrunA17_Chr1g0188491 | 47.154 | 123 | 62 | 3 | 173 | 294 | 6 | 126 | 9.53e-28 | 104 |
| Msa0800270 | MtrunA17_Chr1g0172741 | 28.205 | 312 | 196 | 9 | 1 | 288 | 4 | 311 | 1.74e-27 | 108 |
| Msa0800270 | MtrunA17_Chr2g0285481 | 27.885 | 312 | 197 | 8 | 1 | 288 | 4 | 311 | 9.51e-27 | 107 |
| Msa0800270 | MtrunA17_Chr8g0334551 | 27.987 | 318 | 192 | 9 | 3 | 288 | 5 | 317 | 4.24e-26 | 105 |
| Msa0800270 | MtrunA17_Chr3g0093841 | 37.190 | 121 | 76 | 0 | 22 | 142 | 191 | 311 | 1.35e-19 | 89.0 |
| Msa0800270 | MtrunA17_Chr3g0093841 | 31.092 | 119 | 82 | 0 | 23 | 141 | 27 | 145 | 6.01e-17 | 81.3 |
| Msa0800270 | MtrunA17_Chr3g0093841 | 23.826 | 298 | 193 | 4 | 23 | 288 | 518 | 813 | 7.94e-17 | 80.9 |
| Msa0800270 | MtrunA17_Chr3g0093841 | 31.707 | 123 | 82 | 1 | 22 | 142 | 351 | 473 | 1.34e-15 | 77.0 |
| Msa0800270 | MtrunA17_Chr8g0360271 | 23.404 | 282 | 202 | 5 | 23 | 290 | 13 | 294 | 7.38e-19 | 84.7 |
| Msa0800270 | MtrunA17_Chr7g0264841 | 22.973 | 296 | 195 | 9 | 1 | 279 | 11 | 290 | 2.64e-16 | 77.8 |
| Msa0800270 | MtrunA17_Chr8g0354231 | 20.775 | 284 | 206 | 6 | 23 | 291 | 22 | 301 | 2.21e-14 | 72.4 |
| Msa0800270 | MtrunA17_Chr5g0402821 | 26.014 | 296 | 181 | 9 | 20 | 280 | 16 | 308 | 1.51e-13 | 70.1 |
| Msa0800270 | MtrunA17_Chr8g0362511 | 24.752 | 303 | 171 | 10 | 20 | 283 | 68 | 352 | 1.77e-13 | 70.1 |
| Msa0800270 | MtrunA17_Chr3g0087491 | 26.429 | 280 | 187 | 8 | 27 | 288 | 1 | 279 | 6.33e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0800270 | AT2G24960.1 | 34.507 | 142 | 93 | 0 | 17 | 158 | 9 | 150 | 3.19e-29 | 117 |
| Msa0800270 | AT2G24960.1 | 34.848 | 132 | 86 | 0 | 17 | 148 | 166 | 297 | 6.16e-22 | 96.3 |
| Msa0800270 | AT2G24960.1 | 26.335 | 281 | 185 | 5 | 22 | 297 | 533 | 796 | 3.40e-21 | 94.4 |
| Msa0800270 | AT2G24960.1 | 27.322 | 183 | 110 | 1 | 2 | 161 | 306 | 488 | 5.46e-20 | 90.5 |
| Msa0800270 | AT2G24960.2 | 34.507 | 142 | 93 | 0 | 17 | 158 | 9 | 150 | 3.46e-29 | 117 |
| Msa0800270 | AT2G24960.2 | 31.250 | 160 | 110 | 0 | 2 | 161 | 306 | 465 | 1.86e-24 | 103 |
| Msa0800270 | AT2G24960.2 | 34.848 | 132 | 86 | 0 | 17 | 148 | 166 | 297 | 5.87e-22 | 96.3 |
| Msa0800270 | AT2G24960.2 | 26.335 | 281 | 185 | 5 | 22 | 297 | 510 | 773 | 3.34e-21 | 94.4 |
| Msa0800270 | AT5G05800.1 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0800270 | AT5G05800.1 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0800270 | AT5G05800.5 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0800270 | AT5G05800.5 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0800270 | AT5G05800.3 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0800270 | AT5G05800.3 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0800270 | AT5G05800.2 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0800270 | AT5G05800.2 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0800270 | AT5G05800.4 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0800270 | AT5G05800.4 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0800270 | AT4G02210.1 | 34.965 | 143 | 92 | 1 | 17 | 158 | 8 | 150 | 4.16e-26 | 107 |
| Msa0800270 | AT4G02210.1 | 25.448 | 279 | 181 | 3 | 16 | 291 | 179 | 433 | 7.09e-25 | 103 |
| Msa0800270 | AT4G02210.2 | 34.965 | 143 | 92 | 1 | 17 | 158 | 8 | 150 | 4.16e-26 | 107 |
| Msa0800270 | AT4G02210.2 | 25.448 | 279 | 181 | 3 | 16 | 291 | 179 | 433 | 7.09e-25 | 103 |
| Msa0800270 | AT3G11290.2 | 29.210 | 291 | 185 | 7 | 18 | 290 | 163 | 450 | 6.47e-24 | 101 |
| Msa0800270 | AT3G11290.2 | 26.347 | 167 | 106 | 3 | 20 | 186 | 4 | 153 | 8.60e-12 | 65.9 |
| Msa0800270 | AT3G11290.1 | 29.210 | 291 | 185 | 7 | 18 | 290 | 163 | 450 | 6.47e-24 | 101 |
| Msa0800270 | AT3G11290.1 | 26.347 | 167 | 106 | 3 | 20 | 186 | 4 | 153 | 8.60e-12 | 65.9 |
| Msa0800270 | AT2G19220.1 | 27.575 | 301 | 177 | 12 | 10 | 288 | 151 | 432 | 9.28e-18 | 83.6 |
| Msa0800270 | AT4G02550.1 | 28.986 | 138 | 97 | 1 | 23 | 159 | 22 | 159 | 2.00e-16 | 78.6 |
| Msa0800270 | AT4G02550.5 | 28.986 | 138 | 97 | 1 | 23 | 159 | 22 | 159 | 2.00e-16 | 78.6 |
| Msa0800270 | AT4G02550.3 | 28.986 | 138 | 97 | 1 | 23 | 159 | 37 | 174 | 2.58e-16 | 78.2 |
| Msa0800270 | AT4G02550.2 | 23.443 | 273 | 184 | 6 | 23 | 291 | 22 | 273 | 3.01e-15 | 74.7 |
| Msa0800270 | AT4G02550.4 | 23.443 | 273 | 184 | 6 | 23 | 291 | 22 | 273 | 3.01e-15 | 74.7 |
| Msa0800270 | AT3G11310.1 | 28.916 | 166 | 98 | 5 | 16 | 166 | 169 | 329 | 2.91e-12 | 67.4 |
Find 55 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATTGCGAATGATGAAATT+TGG | 0.147259 | 5_3:-13646190 | Msa0800270:CDS |
| GTTTATTCCCTTTGTGTACT+TGG | 0.221915 | 5_3:+13646396 | None:intergenic |
| TGGTGGCTGTCCAATCTTTA+AGG | 0.238961 | 5_3:-13645772 | Msa0800270:CDS |
| TGATGTGTGCTCAACAATTC+TGG | 0.281107 | 5_3:+13645660 | None:intergenic |
| TTGATGAAGGTGACTTTGTT+TGG | 0.332628 | 5_3:-13646232 | Msa0800270:CDS |
| TAAGATACTGGCAGATTTGA+TGG | 0.340898 | 5_3:-13646423 | Msa0800270:CDS |
| TTTAGAGTTATGTTGCTCTA+TGG | 0.360492 | 5_3:+13645461 | None:intergenic |
| AATTGCGAATGATGAAATTT+GGG | 0.361414 | 5_3:-13646189 | Msa0800270:CDS |
| TGATGAAGGTGACTTTGTTT+GGG | 0.364398 | 5_3:-13646231 | Msa0800270:CDS |
| CAAGTTTCTGTAGGTGTAGT+AGG | 0.385419 | 5_3:+13645576 | None:intergenic |
| GCCTTCTTGATCTTGTAATT+CGG | 0.395466 | 5_3:+13646498 | None:intergenic |
| GTACATAGCTCCTTAAAGAT+TGG | 0.398875 | 5_3:+13645762 | None:intergenic |
| ATTTGTCTCTTCCTCATCCT+CGG | 0.434458 | 5_3:+13645617 | None:intergenic |
| AAAGTCACCTTCATCAAGAA+TGG | 0.436149 | 5_3:+13646238 | None:intergenic |
| CAGCATATCTGACTGTGTTA+AGG | 0.442168 | 5_3:-13645436 | Msa0800270:CDS |
| AGCATCGCTCACTAAGATAC+TGG | 0.460192 | 5_3:-13646435 | Msa0800270:CDS |
| AGAGGGCGTAAAGGAGTTGA+TGG | 0.467269 | 5_3:-13645540 | Msa0800270:CDS |
| GAGACATTGCCGTTTCTCTC+AGG | 0.473735 | 5_3:-13647683 | Msa0800270:intron |
| GTTTAACTTGGTTGCACCGC+AGG | 0.484247 | 5_3:-13645305 | Msa0800270:CDS |
| AAAAGGATAAACGTTTAACT+TGG | 0.500116 | 5_3:-13645317 | Msa0800270:CDS |
| CGACTACTACAAGTTTCTGT+AGG | 0.502887 | 5_3:+13645567 | None:intergenic |
| GCATGGAAACATATTTGTGA+TGG | 0.508692 | 5_3:-13646350 | Msa0800270:CDS |
| AATTCTGGACAAGGAGCTCT+AGG | 0.508728 | 5_3:+13645675 | None:intergenic |
| TTCTCTGACGCAGCAACGAA+CGG | 0.509181 | 5_3:-13645735 | Msa0800270:CDS |
| AACAGGTCTGAAATGGGATA+AGG | 0.509493 | 5_3:-13646315 | Msa0800270:CDS |
| TTCATCTGAATCCGAGGATG+AGG | 0.517272 | 5_3:-13645628 | Msa0800270:CDS |
| TGCTGTTGTATCCAATAGAT+AGG | 0.518107 | 5_3:-13645280 | Msa0800270:exon |
| AAGGATTTAGATTTAATGCA+AGG | 0.526331 | 5_3:-13645417 | Msa0800270:CDS |
| AAGTCACCTTCATCAAGAAT+GGG | 0.528147 | 5_3:+13646239 | None:intergenic |
| AGTCGCAAAAGAGGGCGTAA+AGG | 0.529428 | 5_3:-13645549 | Msa0800270:CDS |
| CTTTGTTTGGGATGAAGCCA+CGG | 0.540715 | 5_3:-13646219 | Msa0800270:CDS |
| ATTCATTCAATAAGAAAGCA+TGG | 0.542305 | 5_3:-13646367 | Msa0800270:CDS |
| ACTTGTAGTAGTCGCAAAAG+AGG | 0.542854 | 5_3:-13645558 | Msa0800270:CDS |
| TGGTTGACCAAGTACACAAA+GGG | 0.543427 | 5_3:-13646403 | Msa0800270:CDS |
| TAGTAGGTTGAACTGTTTGA+GGG | 0.546289 | 5_3:+13645592 | None:intergenic |
| GTGAATTTACCTGAGAGAAA+CGG | 0.551539 | 5_3:+13647674 | None:intergenic |
| GTGAAGCCCATTCTTGATGA+AGG | 0.552695 | 5_3:-13646245 | Msa0800270:CDS |
| TTTGTTTGGGATGAAGCCAC+GGG | 0.569196 | 5_3:-13646218 | Msa0800270:CDS |
| GTAGTAGGTTGAACTGTTTG+AGG | 0.569252 | 5_3:+13645591 | None:intergenic |
| CTCAGCAGATAGAACAGTCA+AGG | 0.570319 | 5_3:-13646469 | Msa0800270:CDS |
| TGCAGACGCTATATTAGAGA+TGG | 0.575188 | 5_3:-13645511 | Msa0800270:CDS |
| GGCATCTGCTTCAAAGATGA+GGG | 0.578418 | 5_3:-13645490 | Msa0800270:CDS |
| TGGCATCTGCTTCAAAGATG+AGG | 0.587021 | 5_3:-13645491 | Msa0800270:CDS |
| TAGAACAGTCAAGGGCTAAG+TGG | 0.596106 | 5_3:-13646460 | Msa0800270:CDS |
| ATTCGCAATTATAGCTCCCG+TGG | 0.603592 | 5_3:+13646202 | None:intergenic |
| CTTGTAGTAGTCGCAAAAGA+GGG | 0.619141 | 5_3:-13645557 | Msa0800270:CDS |
| GTGCTCAACAATTCTGGACA+AGG | 0.633276 | 5_3:+13645666 | None:intergenic |
| AAAACCGACATTCAGTGTTG+AGG | 0.637621 | 5_3:-13646283 | Msa0800270:CDS |
| TCAGCAGATAGAACAGTCAA+GGG | 0.645530 | 5_3:-13646468 | Msa0800270:CDS |
| TGAGTCTTCATCTGAATCCG+AGG | 0.649200 | 5_3:-13645634 | Msa0800270:CDS |
| ATGGTTGACCAAGTACACAA+AGG | 0.660204 | 5_3:-13646404 | Msa0800270:CDS |
| GTCTCCTCAACACTGAATGT+CGG | 0.665231 | 5_3:+13646279 | None:intergenic |
| GCCGAATTACAAGATCAAGA+AGG | 0.670605 | 5_3:-13646499 | Msa0800270:CDS |
| AATTTGGGAAGAATACATCA+AGG | 0.671593 | 5_3:-13646174 | Msa0800270:intron |
| TGGATACAACAGCACACCTG+CGG | 0.685182 | 5_3:+13645289 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTAAAGAAAAATCAAAAA+TGG | + | chr5_3:13645520-13645539 | None:intergenic | 15.0% |
| !! | AATTCACATACAAAATTTAT+TGG | + | chr5_3:13645624-13645643 | None:intergenic | 15.0% |
| !!! | TATATGAAAAGATATCTTTT+TGG | - | chr5_3:13647623-13647642 | Msa0800270:intron | 15.0% |
| !! | TTGTTAATTGCATATACAAT+GGG | - | chr5_3:13645451-13645470 | Msa0800270:CDS | 20.0% |
| !! | TTTATGTGAGTTTCATTTAT+TGG | - | chr5_3:13645596-13645615 | Msa0800270:CDS | 20.0% |
| !!! | TATAAGATTCTAGTTCATTT+TGG | + | chr5_3:13646208-13646227 | None:intergenic | 20.0% |
| !! | TATGTTAGTTAAACTATAGA+TGG | - | chr5_3:13646347-13646366 | Msa0800270:CDS | 20.0% |
| !! | AATAACAACAAAACAAATGA+TGG | + | chr5_3:13646733-13646752 | None:intergenic | 20.0% |
| !! | ATTAATTGAGTACATCAAAA+TGG | - | chr5_3:13646750-13646769 | Msa0800270:intron | 20.0% |
| !!! | ACTTTTGAATTGTTTCTATT+AGG | - | chr5_3:13646790-13646809 | Msa0800270:intron | 20.0% |
| !!! | ATATTTTTCAACCTATCTAT+TGG | + | chr5_3:13647606-13647625 | None:intergenic | 20.0% |
| ! | TTTCTTCTCTCTTCTTTATT+AGG | - | chr5_3:13645221-13645240 | Msa0800270:three_prime_UTR | 25.0% |
| ! | TTCTTCTCTCTTCTTTATTA+GGG | - | chr5_3:13645222-13645241 | Msa0800270:three_prime_UTR | 25.0% |
| ! | TTCATATGATTCAGAATCAT+GGG | + | chr5_3:13645352-13645371 | None:intergenic | 25.0% |
| ! | GTTGTTAATTGCATATACAA+TGG | - | chr5_3:13645450-13645469 | Msa0800270:CDS | 25.0% |
| ! | AAAATCAAAAATGGAGTACA+TGG | + | chr5_3:13645511-13645530 | None:intergenic | 25.0% |
| ! | TTAAATTGTCTATCCAAACA+GGG | + | chr5_3:13645905-13645924 | None:intergenic | 25.0% |
| ! | ATTAAATTGTCTATCCAAAC+AGG | + | chr5_3:13645906-13645925 | None:intergenic | 25.0% |
| !! | TATGAAATTAGAGACTGTTT+TGG | + | chr5_3:13646135-13646154 | None:intergenic | 25.0% |
| ! | TAAAAATAAGAGGATAACCA+CGG | + | chr5_3:13646255-13646274 | None:intergenic | 25.0% |
| ! | AACATAAGTAATCGATAAAG+AGG | + | chr5_3:13646333-13646352 | None:intergenic | 25.0% |
| ! | ATTCATTCAATAAGAAAGCA+TGG | - | chr5_3:13646505-13646524 | Msa0800270:CDS | 25.0% |
| ! | TAATTGCGAATGATGAAATT+TGG | - | chr5_3:13646682-13646701 | Msa0800270:intron | 25.0% |
| ! | AATTGCGAATGATGAAATTT+GGG | - | chr5_3:13646683-13646702 | Msa0800270:intron | 25.0% |
| ! | GTTATTTATCAGAAGATCAA+CGG | + | chr5_3:13646958-13646977 | None:intergenic | 25.0% |
| ! | AAGGATTTAGATTTAATGCA+AGG | - | chr5_3:13647455-13647474 | Msa0800270:intron | 25.0% |
| ! | AAAAATATCTCTCTTGCATT+AGG | + | chr5_3:13647527-13647546 | None:intergenic | 25.0% |
| !!! | GATATTTTTGTCTCTCAAAA+AGG | - | chr5_3:13647538-13647557 | Msa0800270:intron | 25.0% |
| ! | AAAAGGATAAACGTTTAACT+TGG | - | chr5_3:13647555-13647574 | Msa0800270:intron | 25.0% |
| TCAATTTAAGCATTCTACGT+TGG | + | chr5_3:13645316-13645335 | None:intergenic | 30.0% | |
| CTTCATATGATTCAGAATCA+TGG | + | chr5_3:13645353-13645372 | None:intergenic | 30.0% | |
| !!! | TTGAGATTTTGATTCTTCAC+TGG | - | chr5_3:13645416-13645435 | Msa0800270:CDS | 30.0% |
| AAAATCAAATCTCCAACTGA+AGG | + | chr5_3:13645659-13645678 | None:intergenic | 30.0% | |
| ATGTTCTTTCATTTCTCACA+TGG | - | chr5_3:13645685-13645704 | Msa0800270:CDS | 30.0% | |
| AATGAGCTGAAAGCAATTTA+TGG | - | chr5_3:13646027-13646046 | Msa0800270:intron | 30.0% | |
| AGAATCTTATATCCAAGTAC+TGG | - | chr5_3:13646217-13646236 | Msa0800270:CDS | 30.0% | |
| TGTGCATGCATAAAAATAAG+AGG | + | chr5_3:13646265-13646284 | None:intergenic | 30.0% | |
| ! | GATGGATTTCACAAAAAAAC+AGG | - | chr5_3:13646540-13646559 | Msa0800270:intron | 30.0% |
| ACAAAAAAACAGGTCTGAAA+TGG | - | chr5_3:13646550-13646569 | Msa0800270:intron | 30.0% | |
| ! | CAAAAAAACAGGTCTGAAAT+GGG | - | chr5_3:13646551-13646570 | Msa0800270:intron | 30.0% |
| AATTTGGGAAGAATACATCA+AGG | - | chr5_3:13646698-13646717 | Msa0800270:intron | 30.0% | |
| ! | ACAACAAAACAAATGATGGA+TGG | + | chr5_3:13646729-13646748 | None:intergenic | 30.0% |
| TACAATGGAAAGGAAAGATT+GGG | + | chr5_3:13646988-13647007 | None:intergenic | 30.0% | |
| ATACAATGGAAAGGAAAGAT+TGG | + | chr5_3:13646989-13647008 | None:intergenic | 30.0% | |
| TAGATTGTGCTCAAATACAA+TGG | + | chr5_3:13647003-13647022 | None:intergenic | 30.0% | |
| ! | TTTAGAGTTATGTTGCTCTA+TGG | + | chr5_3:13647414-13647433 | None:intergenic | 30.0% |
| GTGAATTTACCTGAGAGAAA+CGG | + | chr5_3:13645201-13645220 | None:intergenic | 35.0% | |
| ATTTAAGCATTCTACGTTGG+TGG | + | chr5_3:13645313-13645332 | None:intergenic | 35.0% | |
| ! | TTTTTGCCCTTTTCCATTGA+TGG | - | chr5_3:13645379-13645398 | Msa0800270:CDS | 35.0% |
| ! | TTTTGCCCTTTTCCATTGAT+GGG | - | chr5_3:13645380-13645399 | Msa0800270:CDS | 35.0% |
| GCAAAATGAGCCTAATGTTA+GGG | + | chr5_3:13645486-13645505 | None:intergenic | 35.0% | |
| TGCAAAATGAGCCTAATGTT+AGG | + | chr5_3:13645487-13645506 | None:intergenic | 35.0% | |
| AGTACCATATATCCTTCAGT+TGG | - | chr5_3:13645644-13645663 | Msa0800270:CDS | 35.0% | |
| ATCTCCAACTGAAGGATATA+TGG | + | chr5_3:13645651-13645670 | None:intergenic | 35.0% | |
| GAATGCGCAAACAAAAGAAA+AGG | + | chr5_3:13645748-13645767 | None:intergenic | 35.0% | |
| GTTTGCGCATTCAAATATCT+AGG | - | chr5_3:13645756-13645775 | Msa0800270:CDS | 35.0% | |
| CTCATTTCTATAAGCACTCT+AGG | + | chr5_3:13646013-13646032 | None:intergenic | 35.0% | |
| GCCTTCTTGATCTTGTAATT+CGG | + | chr5_3:13646377-13646396 | None:intergenic | 35.0% | |
| TAAGATACTGGCAGATTTGA+TGG | - | chr5_3:13646449-13646468 | Msa0800270:CDS | 35.0% | |
| GTTTATTCCCTTTGTGTACT+TGG | + | chr5_3:13646479-13646498 | None:intergenic | 35.0% | |
| GCATGGAAACATATTTGTGA+TGG | - | chr5_3:13646522-13646541 | Msa0800270:intron | 35.0% | |
| AAGTCACCTTCATCAAGAAT+GGG | + | chr5_3:13646636-13646655 | None:intergenic | 35.0% | |
| AAAGTCACCTTCATCAAGAA+TGG | + | chr5_3:13646637-13646656 | None:intergenic | 35.0% | |
| TTGATGAAGGTGACTTTGTT+TGG | - | chr5_3:13646640-13646659 | Msa0800270:intron | 35.0% | |
| ! | TGATGAAGGTGACTTTGTTT+GGG | - | chr5_3:13646641-13646660 | Msa0800270:intron | 35.0% |
| TTTACCCCTCTTCTATAGTA+AGG | - | chr5_3:13646814-13646833 | Msa0800270:intron | 35.0% | |
| ACAATGGAAAGGAAAGATTG+GGG | + | chr5_3:13646987-13647006 | None:intergenic | 35.0% | |
| TGTGCTCAAATACAATGGAA+AGG | + | chr5_3:13646998-13647017 | None:intergenic | 35.0% | |
| GTACATAGCTCCTTAAAGAT+TGG | + | chr5_3:13647113-13647132 | None:intergenic | 35.0% | |
| ! | TAGTAGGTTGAACTGTTTGA+GGG | + | chr5_3:13647283-13647302 | None:intergenic | 35.0% |
| TGCTGTTGTATCCAATAGAT+AGG | - | chr5_3:13647592-13647611 | Msa0800270:intron | 35.0% | |
| ! | TTTGCCCTTTTCCATTGATG+GGG | - | chr5_3:13645381-13645400 | Msa0800270:CDS | 40.0% |
| GAAACCCCATCAATGGAAAA+GGG | + | chr5_3:13645388-13645407 | None:intergenic | 40.0% | |
| AGAAACCCCATCAATGGAAA+AGG | + | chr5_3:13645389-13645408 | None:intergenic | 40.0% | |
| AATCAGAGAAACCCCATCAA+TGG | + | chr5_3:13645395-13645414 | None:intergenic | 40.0% | |
| GCTATCACTACCCTAACATT+AGG | - | chr5_3:13645473-13645492 | Msa0800270:CDS | 40.0% | |
| CTGTGATTCATCATGGTCTA+AGG | - | chr5_3:13645879-13645898 | Msa0800270:intron | 40.0% | |
| AGACTGTTTGTGAGAGCTTA+TGG | + | chr5_3:13646071-13646090 | None:intergenic | 40.0% | |
| CAAGAGTGAATTCCAGTACT+TGG | + | chr5_3:13646232-13646251 | None:intergenic | 40.0% | |
| GCCGAATTACAAGATCAAGA+AGG | - | chr5_3:13646373-13646392 | Msa0800270:CDS | 40.0% | |
| TCAGCAGATAGAACAGTCAA+GGG | - | chr5_3:13646404-13646423 | Msa0800270:CDS | 40.0% | |
| ATGGTTGACCAAGTACACAA+AGG | - | chr5_3:13646468-13646487 | Msa0800270:CDS | 40.0% | |
| TGGTTGACCAAGTACACAAA+GGG | - | chr5_3:13646469-13646488 | Msa0800270:CDS | 40.0% | |
| ! | AACAGGTCTGAAATGGGATA+AGG | - | chr5_3:13646557-13646576 | Msa0800270:intron | 40.0% |
| AAAACCGACATTCAGTGTTG+AGG | - | chr5_3:13646589-13646608 | Msa0800270:intron | 40.0% | |
| CAAAAGTTTCCTCGTGAAGA+AGG | + | chr5_3:13646777-13646796 | None:intergenic | 40.0% | |
| AGCTCCTTACTATAGAAGAG+GGG | + | chr5_3:13646821-13646840 | None:intergenic | 40.0% | |
| GAGCTCCTTACTATAGAAGA+GGG | + | chr5_3:13646822-13646841 | None:intergenic | 40.0% | |
| AGAGCTCCTTACTATAGAAG+AGG | + | chr5_3:13646823-13646842 | None:intergenic | 40.0% | |
| TCTACCTAAGAGGGAAAAGT+GGG | - | chr5_3:13646840-13646859 | Msa0800270:intron | 40.0% | |
| TGATGTGTGCTCAACAATTC+TGG | + | chr5_3:13647215-13647234 | None:intergenic | 40.0% | |
| ATTTGTCTCTTCCTCATCCT+CGG | + | chr5_3:13647258-13647277 | None:intergenic | 40.0% | |
| ! | GTAGTAGGTTGAACTGTTTG+AGG | + | chr5_3:13647284-13647303 | None:intergenic | 40.0% |
| CAAGTTTCTGTAGGTGTAGT+AGG | + | chr5_3:13647299-13647318 | None:intergenic | 40.0% | |
| CGACTACTACAAGTTTCTGT+AGG | + | chr5_3:13647308-13647327 | None:intergenic | 40.0% | |
| ACTTGTAGTAGTCGCAAAAG+AGG | - | chr5_3:13647314-13647333 | Msa0800270:intron | 40.0% | |
| CTTGTAGTAGTCGCAAAAGA+GGG | - | chr5_3:13647315-13647334 | Msa0800270:intron | 40.0% | |
| TGCAGACGCTATATTAGAGA+TGG | - | chr5_3:13647361-13647380 | Msa0800270:intron | 40.0% | |
| CAGCATATCTGACTGTGTTA+AGG | - | chr5_3:13647436-13647455 | Msa0800270:intron | 40.0% | |
| CACAAAAATCCCTCCACAAC+AGG | + | chr5_3:13645802-13645821 | None:intergenic | 45.0% | |
| ! | GGGATTTTTGTGCTCTTCTG+TGG | - | chr5_3:13645810-13645829 | Msa0800270:intron | 45.0% |
| ACCATGATGAATCACAGCGA+GGG | + | chr5_3:13645876-13645895 | None:intergenic | 45.0% | |
| CTCAGCAGATAGAACAGTCA+AGG | - | chr5_3:13646403-13646422 | Msa0800270:CDS | 45.0% | |
| TAGAACAGTCAAGGGCTAAG+TGG | - | chr5_3:13646412-13646431 | Msa0800270:CDS | 45.0% | |
| AGCATCGCTCACTAAGATAC+TGG | - | chr5_3:13646437-13646456 | Msa0800270:CDS | 45.0% | |
| GTCTCCTCAACACTGAATGT+CGG | + | chr5_3:13646596-13646615 | None:intergenic | 45.0% | |
| GTGAAGCCCATTCTTGATGA+AGG | - | chr5_3:13646627-13646646 | Msa0800270:intron | 45.0% | |
| ! | CTTTGTTTGGGATGAAGCCA+CGG | - | chr5_3:13646653-13646672 | Msa0800270:intron | 45.0% |
| ! | TTTGTTTGGGATGAAGCCAC+GGG | - | chr5_3:13646654-13646673 | Msa0800270:intron | 45.0% |
| ATTCGCAATTATAGCTCCCG+TGG | + | chr5_3:13646673-13646692 | None:intergenic | 45.0% | |
| CAAAATGGTCCTTCTTCACG+AGG | - | chr5_3:13646765-13646784 | Msa0800270:intron | 45.0% | |
| AGTAAGGAGCTCTACCTAAG+AGG | - | chr5_3:13646830-13646849 | Msa0800270:intron | 45.0% | |
| GTAAGGAGCTCTACCTAAGA+GGG | - | chr5_3:13646831-13646850 | Msa0800270:intron | 45.0% | |
| CTCTACCTAAGAGGGAAAAG+TGG | - | chr5_3:13646839-13646858 | Msa0800270:intron | 45.0% | |
| CTACCTAAGAGGGAAAAGTG+GGG | - | chr5_3:13646841-13646860 | Msa0800270:intron | 45.0% | |
| !!! | GCCACCACTTTTTACAGTCT+CGG | + | chr5_3:13647087-13647106 | None:intergenic | 45.0% |
| TGGTGGCTGTCCAATCTTTA+AGG | - | chr5_3:13647100-13647119 | Msa0800270:intron | 45.0% | |
| AATTCTGGACAAGGAGCTCT+AGG | + | chr5_3:13647200-13647219 | None:intergenic | 45.0% | |
| GTGCTCAACAATTCTGGACA+AGG | + | chr5_3:13647209-13647228 | None:intergenic | 45.0% | |
| TGAGTCTTCATCTGAATCCG+AGG | - | chr5_3:13647238-13647257 | Msa0800270:intron | 45.0% | |
| TTCATCTGAATCCGAGGATG+AGG | - | chr5_3:13647244-13647263 | Msa0800270:intron | 45.0% | |
| TGGCATCTGCTTCAAAGATG+AGG | - | chr5_3:13647381-13647400 | Msa0800270:intron | 45.0% | |
| GGCATCTGCTTCAAAGATGA+GGG | - | chr5_3:13647382-13647401 | Msa0800270:intron | 45.0% | |
| ! | GAGACATTGCCGTTTCTCTC+AGG | - | chr5_3:13645189-13645208 | Msa0800270:three_prime_UTR | 50.0% |
| AGTTGTGAGTCCTGTTGTGG+AGG | - | chr5_3:13645789-13645808 | Msa0800270:CDS | 50.0% | |
| GTTGTGAGTCCTGTTGTGGA+GGG | - | chr5_3:13645790-13645809 | Msa0800270:CDS | 50.0% | |
| GACCATGATGAATCACAGCG+AGG | + | chr5_3:13645877-13645896 | None:intergenic | 50.0% | |
| ! | TCATGGTCTAAGGCCCTGTT+TGG | - | chr5_3:13645889-13645908 | Msa0800270:intron | 50.0% |
| ACTGGAATTCACTCTTGCCG+TGG | - | chr5_3:13646235-13646254 | Msa0800270:CDS | 50.0% | |
| !! | ACTCCCCACTTTTCCCTCTT+AGG | + | chr5_3:13646847-13646866 | None:intergenic | 50.0% |
| ! | CTTTTTACAGTCTCGGCGTC+AGG | + | chr5_3:13647080-13647099 | None:intergenic | 50.0% |
| GACGCCGAGACTGTAAAAAG+TGG | - | chr5_3:13647080-13647099 | Msa0800270:intron | 50.0% | |
| GCCGAGACTGTAAAAAGTGG+TGG | - | chr5_3:13647083-13647102 | Msa0800270:intron | 50.0% | |
| TTCTCTGACGCAGCAACGAA+CGG | - | chr5_3:13647137-13647156 | Msa0800270:intron | 50.0% | |
| ! | AGTCGCAAAAGAGGGCGTAA+AGG | - | chr5_3:13647323-13647342 | Msa0800270:intron | 50.0% |
| ! | AGAGGGCGTAAAGGAGTTGA+TGG | - | chr5_3:13647332-13647351 | Msa0800270:intron | 50.0% |
| !! | GTTTAACTTGGTTGCACCGC+AGG | - | chr5_3:13647567-13647586 | Msa0800270:intron | 50.0% |
| TGGATACAACAGCACACCTG+CGG | + | chr5_3:13647586-13647605 | None:intergenic | 50.0% | |
| GCGAGTTGTGAGTCCTGTTG+TGG | - | chr5_3:13645786-13645805 | Msa0800270:CDS | 55.0% | |
| GCCCTCGCTGTGATTCATCA+TGG | - | chr5_3:13645872-13645891 | Msa0800270:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 13645175 | 13647719 | 13645175 | ID=Msa0800270;Name=Msa0800270 |
| chr5_3 | mRNA | 13645175 | 13647719 | 13645175 | ID=Msa0800270-mRNA-1;Parent=Msa0800270;Name=Msa0800270-mRNA-1;_AED=0.03;_eAED=0.03;_QI=36|1|1|1|0.5|0.66|3|106|298 |
| chr5_3 | exon | 13645175 | 13645823 | 13645175 | ID=Msa0800270-mRNA-1:exon:5655;Parent=Msa0800270-mRNA-1 |
| chr5_3 | exon | 13646175 | 13646528 | 13646175 | ID=Msa0800270-mRNA-1:exon:5654;Parent=Msa0800270-mRNA-1 |
| chr5_3 | exon | 13647684 | 13647719 | 13647684 | ID=Msa0800270-mRNA-1:exon:5653;Parent=Msa0800270-mRNA-1 |
| chr5_3 | five_prime_UTR | 13647684 | 13647719 | 13647684 | ID=Msa0800270-mRNA-1:five_prime_utr;Parent=Msa0800270-mRNA-1 |
| chr5_3 | CDS | 13646175 | 13646528 | 13646175 | ID=Msa0800270-mRNA-1:cds;Parent=Msa0800270-mRNA-1 |
| chr5_3 | CDS | 13645281 | 13645823 | 13645281 | ID=Msa0800270-mRNA-1:cds;Parent=Msa0800270-mRNA-1 |
| chr5_3 | three_prime_UTR | 13645175 | 13645280 | 13645175 | ID=Msa0800270-mRNA-1:three_prime_utr;Parent=Msa0800270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
