Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0838260 | RHN54350.1 | 95.302 | 298 | 14 | 0 | 1 | 298 | 31 | 328 | 0.0 | 576 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0838260 | sp|Q9FFJ8|LIMYB_ARATH | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.15e-27 | 113 |
| Msa0838260 | sp|Q9FFJ8|LIMYB_ARATH | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.81e-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0838260 | A0A396HLV9 | 95.302 | 298 | 14 | 0 | 1 | 298 | 31 | 328 | 0.0 | 576 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030280 | Msa0838260 | 0.813445 | 2.549883e-51 | -8.615850e-47 |
| Msa0032260 | Msa0838260 | 0.811423 | 7.053505e-51 | -8.615850e-47 |
| Msa0033110 | Msa0838260 | 0.804761 | 1.849641e-49 | -8.615850e-47 |
| Msa0040690 | Msa0838260 | 0.819737 | 9.924092e-53 | -8.615850e-47 |
| Msa0047160 | Msa0838260 | 0.802346 | 5.859312e-49 | -8.615850e-47 |
| Msa0048180 | Msa0838260 | 0.804791 | 1.822938e-49 | -8.615850e-47 |
| Msa0052610 | Msa0838260 | 0.845518 | 3.863994e-59 | -8.615850e-47 |
| Msa0067870 | Msa0838260 | 0.827207 | 1.776914e-54 | -8.615850e-47 |
| Msa0077880 | Msa0838260 | 0.833269 | 5.874670e-56 | -8.615850e-47 |
| Msa0097850 | Msa0838260 | 0.833284 | 5.824638e-56 | -8.615850e-47 |
| Msa0615440 | Msa0838260 | 0.806321 | 8.706886e-50 | -8.615850e-47 |
| Msa0641140 | Msa0838260 | 0.822973 | 1.778541e-53 | -8.615850e-47 |
| Msa0691900 | Msa0838260 | 0.823960 | 1.045271e-53 | -8.615850e-47 |
| Msa0698490 | Msa0838260 | 0.805232 | 1.474162e-49 | -8.615850e-47 |
| Msa0698660 | Msa0838260 | 0.805543 | 1.268964e-49 | -8.615850e-47 |
| Msa0708520 | Msa0838260 | 0.804516 | 2.080892e-49 | -8.615850e-47 |
| Msa0791240 | Msa0838260 | 0.800903 | 1.158543e-48 | -8.615850e-47 |
| Msa0794050 | Msa0838260 | 0.800445 | 1.436502e-48 | -8.615850e-47 |
| Msa0800270 | Msa0838260 | 0.976162 | 2.661598e-141 | -8.615850e-47 |
| Msa0818770 | Msa0838260 | 0.800706 | 1.270587e-48 | -8.615850e-47 |
| Msa0830390 | Msa0838260 | 0.802739 | 4.862666e-49 | -8.615850e-47 |
| Msa0838260 | Msa0906780 | 0.823470 | 1.361491e-53 | -8.615850e-47 |
| Msa0838260 | Msa0944200 | 0.801461 | 8.908801e-49 | -8.615850e-47 |
| Msa0838260 | Msa1006840 | 0.814807 | 1.276512e-51 | -8.615850e-47 |
| Msa0838260 | Msa1033660 | 0.800100 | 1.688492e-48 | -8.615850e-47 |
| Msa0838260 | Msa1041520 | 0.812138 | 4.928408e-51 | -8.615850e-47 |
| Msa0838260 | Msa1079230 | 0.810013 | 1.423437e-50 | -8.615850e-47 |
| Msa0838260 | Msa1080640 | 0.814191 | 1.746572e-51 | -8.615850e-47 |
| Msa0838260 | Msa1080800 | 0.801600 | 8.339701e-49 | -8.615850e-47 |
| Msa0838260 | Msa1093330 | 0.804447 | 2.150975e-49 | -8.615850e-47 |
| Msa0838260 | Msa1104140 | 0.804792 | 1.822386e-49 | -8.615850e-47 |
| Msa0838260 | Msa1118940 | 0.820842 | 5.540487e-53 | -8.615850e-47 |
| Msa0838260 | Msa1131560 | 0.808427 | 3.113892e-50 | -8.615850e-47 |
| Msa0838260 | Msa1135890 | 0.817692 | 2.890862e-52 | -8.615850e-47 |
| Msa0838260 | Msa1163540 | 0.800963 | 1.126123e-48 | -8.615850e-47 |
| Msa0838260 | Msa1179980 | 0.802471 | 5.522199e-49 | -8.615850e-47 |
| Msa0838260 | Msa1226610 | 0.814189 | 1.748869e-51 | -8.615850e-47 |
| Msa0838260 | Msa1270620 | 0.804192 | 2.431211e-49 | -8.615850e-47 |
| Msa0838260 | Msa1270690 | 0.802308 | 5.967272e-49 | -8.615850e-47 |
| Msa0838260 | Msa1306990 | 0.800339 | 1.509597e-48 | -8.615850e-47 |
| Msa0838260 | Msa1307600 | 0.811143 | 8.112323e-51 | -8.615850e-47 |
| Msa0838260 | Msa1311960 | 0.808854 | 2.523708e-50 | -8.615850e-47 |
| Msa0838260 | Msa1360180 | 0.964238 | 4.471520e-123 | -8.615850e-47 |
| Msa0838260 | Msa1360980 | 0.832008 | 1.207543e-55 | -8.615850e-47 |
| Msa0838260 | Msa1370620 | 0.821209 | 4.560087e-53 | -8.615850e-47 |
| Msa0838260 | Msa1376090 | -0.801321 | 9.513409e-49 | -8.615850e-47 |
| Msa0838260 | Msa1386710 | 0.800277 | 1.554105e-48 | -8.615850e-47 |
| Msa0838260 | Msa1416370 | 0.803881 | 2.821505e-49 | -8.615850e-47 |
| Msa0838260 | Msa1423350 | 0.810198 | 1.298636e-50 | -8.615850e-47 |
| Msa0838260 | Msa1438050 | 0.809581 | 1.762855e-50 | -8.615850e-47 |
| Msa0838260 | Msa1442700 | 0.807801 | 4.233155e-50 | -8.615850e-47 |
| Msa0838260 | Msa1444310 | 0.811484 | 6.842832e-51 | -8.615850e-47 |
| Msa0838260 | Msa1449340 | -0.801424 | 9.063700e-49 | -8.615850e-47 |
| Msa0838260 | Msa1465680 | 0.846374 | 2.260512e-59 | -8.615850e-47 |
| Msa0125770 | Msa0838260 | 0.845289 | 4.457288e-59 | -8.615850e-47 |
| Msa0126770 | Msa0838260 | 0.805361 | 1.385553e-49 | -8.615850e-47 |
| Msa0127310 | Msa0838260 | 0.801931 | 7.132763e-49 | -8.615850e-47 |
| Msa0170410 | Msa0838260 | 0.835489 | 1.629350e-56 | -8.615850e-47 |
| Msa0177210 | Msa0838260 | 0.805142 | 1.539590e-49 | -8.615850e-47 |
| Msa0189300 | Msa0838260 | 0.802945 | 4.409444e-49 | -8.615850e-47 |
| Msa0227400 | Msa0838260 | 0.811805 | 5.827300e-51 | -8.615850e-47 |
| Msa0231660 | Msa0838260 | 0.803524 | 3.345961e-49 | -8.615850e-47 |
| Msa0365860 | Msa0838260 | 0.802999 | 4.297286e-49 | -8.615850e-47 |
| Msa0278790 | Msa0838260 | 0.824198 | 9.190584e-54 | -8.615850e-47 |
| Msa0281430 | Msa0838260 | 0.801801 | 7.584293e-49 | -8.615850e-47 |
| Msa0292120 | Msa0838260 | 0.802365 | 5.808977e-49 | -8.615850e-47 |
| Msa0363680 | Msa0838260 | 0.802762 | 4.809428e-49 | -8.615850e-47 |
| Msa0545060 | Msa0838260 | 0.814884 | 1.227041e-51 | -8.615850e-47 |
| Msa0600600 | Msa0838260 | 0.827341 | 1.650342e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0838260 | MtrunA17_Chr5g0405761 | 95.302 | 298 | 14 | 0 | 1 | 298 | 31 | 328 | 0.0 | 576 |
| Msa0838260 | MtrunA17_Chr1g0156481 | 40.333 | 300 | 153 | 9 | 17 | 298 | 12 | 303 | 8.24e-62 | 198 |
| Msa0838260 | MtrunA17_Chr1g0176881 | 31.915 | 188 | 112 | 3 | 16 | 200 | 18 | 192 | 1.22e-28 | 115 |
| Msa0838260 | MtrunA17_Chr1g0176881 | 32.031 | 128 | 87 | 0 | 18 | 145 | 191 | 318 | 2.25e-22 | 97.1 |
| Msa0838260 | MtrunA17_Chr3g0113081 | 28.475 | 295 | 181 | 6 | 3 | 294 | 366 | 633 | 6.79e-28 | 113 |
| Msa0838260 | MtrunA17_Chr3g0113081 | 30.047 | 213 | 135 | 4 | 3 | 205 | 164 | 372 | 5.23e-24 | 102 |
| Msa0838260 | MtrunA17_Chr3g0113081 | 34.959 | 123 | 80 | 0 | 19 | 141 | 7 | 129 | 5.39e-18 | 84.3 |
| Msa0838260 | MtrunA17_Chr1g0188491 | 47.154 | 123 | 62 | 3 | 173 | 294 | 6 | 126 | 9.53e-28 | 104 |
| Msa0838260 | MtrunA17_Chr1g0172741 | 28.205 | 312 | 196 | 9 | 1 | 288 | 4 | 311 | 1.74e-27 | 108 |
| Msa0838260 | MtrunA17_Chr2g0285481 | 27.885 | 312 | 197 | 8 | 1 | 288 | 4 | 311 | 9.51e-27 | 107 |
| Msa0838260 | MtrunA17_Chr8g0334551 | 27.987 | 318 | 192 | 9 | 3 | 288 | 5 | 317 | 4.24e-26 | 105 |
| Msa0838260 | MtrunA17_Chr3g0093841 | 37.190 | 121 | 76 | 0 | 22 | 142 | 191 | 311 | 1.35e-19 | 89.0 |
| Msa0838260 | MtrunA17_Chr3g0093841 | 31.092 | 119 | 82 | 0 | 23 | 141 | 27 | 145 | 6.01e-17 | 81.3 |
| Msa0838260 | MtrunA17_Chr3g0093841 | 23.826 | 298 | 193 | 4 | 23 | 288 | 518 | 813 | 7.94e-17 | 80.9 |
| Msa0838260 | MtrunA17_Chr3g0093841 | 31.707 | 123 | 82 | 1 | 22 | 142 | 351 | 473 | 1.34e-15 | 77.0 |
| Msa0838260 | MtrunA17_Chr8g0360271 | 23.404 | 282 | 202 | 5 | 23 | 290 | 13 | 294 | 7.38e-19 | 84.7 |
| Msa0838260 | MtrunA17_Chr7g0264841 | 22.973 | 296 | 195 | 9 | 1 | 279 | 11 | 290 | 2.64e-16 | 77.8 |
| Msa0838260 | MtrunA17_Chr8g0354231 | 20.775 | 284 | 206 | 6 | 23 | 291 | 22 | 301 | 2.21e-14 | 72.4 |
| Msa0838260 | MtrunA17_Chr5g0402821 | 26.014 | 296 | 181 | 9 | 20 | 280 | 16 | 308 | 1.51e-13 | 70.1 |
| Msa0838260 | MtrunA17_Chr8g0362511 | 24.752 | 303 | 171 | 10 | 20 | 283 | 68 | 352 | 1.77e-13 | 70.1 |
| Msa0838260 | MtrunA17_Chr3g0087491 | 26.429 | 280 | 187 | 8 | 27 | 288 | 1 | 279 | 6.33e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0838260 | AT2G24960.1 | 34.507 | 142 | 93 | 0 | 17 | 158 | 9 | 150 | 3.19e-29 | 117 |
| Msa0838260 | AT2G24960.1 | 34.848 | 132 | 86 | 0 | 17 | 148 | 166 | 297 | 6.16e-22 | 96.3 |
| Msa0838260 | AT2G24960.1 | 26.335 | 281 | 185 | 5 | 22 | 297 | 533 | 796 | 3.40e-21 | 94.4 |
| Msa0838260 | AT2G24960.1 | 27.322 | 183 | 110 | 1 | 2 | 161 | 306 | 488 | 5.46e-20 | 90.5 |
| Msa0838260 | AT2G24960.2 | 34.507 | 142 | 93 | 0 | 17 | 158 | 9 | 150 | 3.46e-29 | 117 |
| Msa0838260 | AT2G24960.2 | 31.250 | 160 | 110 | 0 | 2 | 161 | 306 | 465 | 1.86e-24 | 103 |
| Msa0838260 | AT2G24960.2 | 34.848 | 132 | 86 | 0 | 17 | 148 | 166 | 297 | 5.87e-22 | 96.3 |
| Msa0838260 | AT2G24960.2 | 26.335 | 281 | 185 | 5 | 22 | 297 | 510 | 773 | 3.34e-21 | 94.4 |
| Msa0838260 | AT5G05800.1 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0838260 | AT5G05800.1 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0838260 | AT5G05800.5 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0838260 | AT5G05800.5 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0838260 | AT5G05800.3 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0838260 | AT5G05800.3 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0838260 | AT5G05800.2 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0838260 | AT5G05800.2 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0838260 | AT5G05800.4 | 28.866 | 291 | 171 | 7 | 18 | 290 | 165 | 437 | 3.21e-28 | 113 |
| Msa0838260 | AT5G05800.4 | 29.268 | 123 | 87 | 0 | 20 | 142 | 4 | 126 | 8.95e-15 | 74.7 |
| Msa0838260 | AT4G02210.1 | 34.965 | 143 | 92 | 1 | 17 | 158 | 8 | 150 | 4.16e-26 | 107 |
| Msa0838260 | AT4G02210.1 | 25.448 | 279 | 181 | 3 | 16 | 291 | 179 | 433 | 7.09e-25 | 103 |
| Msa0838260 | AT4G02210.2 | 34.965 | 143 | 92 | 1 | 17 | 158 | 8 | 150 | 4.16e-26 | 107 |
| Msa0838260 | AT4G02210.2 | 25.448 | 279 | 181 | 3 | 16 | 291 | 179 | 433 | 7.09e-25 | 103 |
| Msa0838260 | AT3G11290.2 | 29.210 | 291 | 185 | 7 | 18 | 290 | 163 | 450 | 6.47e-24 | 101 |
| Msa0838260 | AT3G11290.2 | 26.347 | 167 | 106 | 3 | 20 | 186 | 4 | 153 | 8.60e-12 | 65.9 |
| Msa0838260 | AT3G11290.1 | 29.210 | 291 | 185 | 7 | 18 | 290 | 163 | 450 | 6.47e-24 | 101 |
| Msa0838260 | AT3G11290.1 | 26.347 | 167 | 106 | 3 | 20 | 186 | 4 | 153 | 8.60e-12 | 65.9 |
| Msa0838260 | AT2G19220.1 | 27.575 | 301 | 177 | 12 | 10 | 288 | 151 | 432 | 9.28e-18 | 83.6 |
| Msa0838260 | AT4G02550.1 | 28.986 | 138 | 97 | 1 | 23 | 159 | 22 | 159 | 2.00e-16 | 78.6 |
| Msa0838260 | AT4G02550.5 | 28.986 | 138 | 97 | 1 | 23 | 159 | 22 | 159 | 2.00e-16 | 78.6 |
| Msa0838260 | AT4G02550.3 | 28.986 | 138 | 97 | 1 | 23 | 159 | 37 | 174 | 2.58e-16 | 78.2 |
| Msa0838260 | AT4G02550.2 | 23.443 | 273 | 184 | 6 | 23 | 291 | 22 | 273 | 3.01e-15 | 74.7 |
| Msa0838260 | AT4G02550.4 | 23.443 | 273 | 184 | 6 | 23 | 291 | 22 | 273 | 3.01e-15 | 74.7 |
| Msa0838260 | AT3G11310.1 | 28.916 | 166 | 98 | 5 | 16 | 166 | 169 | 329 | 2.91e-12 | 67.4 |
Find 55 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATTGCGAATGATGAAATT+TGG | 0.147259 | 5_4:-12636148 | Msa0838260:CDS |
| GTTTATTCCCTTTGTGTACT+TGG | 0.221915 | 5_4:+12636354 | None:intergenic |
| TGGTGGCTGTCCAATCTTTA+AGG | 0.238961 | 5_4:-12635730 | Msa0838260:CDS |
| TGATGTGTGCTCAACAATTC+TGG | 0.281107 | 5_4:+12635618 | None:intergenic |
| TTGATGAAGGTGACTTTGTT+TGG | 0.332628 | 5_4:-12636190 | Msa0838260:CDS |
| TAAGATACTGGCAGATTTGA+TGG | 0.340898 | 5_4:-12636381 | Msa0838260:CDS |
| TTTAGAGTTATGTTGCTCTA+TGG | 0.360492 | 5_4:+12635419 | None:intergenic |
| AATTGCGAATGATGAAATTT+GGG | 0.361414 | 5_4:-12636147 | Msa0838260:CDS |
| TGATGAAGGTGACTTTGTTT+GGG | 0.364398 | 5_4:-12636189 | Msa0838260:CDS |
| CAAGTTTCTGTAGGTGTAGT+AGG | 0.385419 | 5_4:+12635534 | None:intergenic |
| GCCTTCTTGATCTTGTAATT+CGG | 0.395466 | 5_4:+12636456 | None:intergenic |
| GTACATAGCTCCTTAAAGAT+TGG | 0.398875 | 5_4:+12635720 | None:intergenic |
| ATTTGTCTCTTCCTCATCCT+CGG | 0.434458 | 5_4:+12635575 | None:intergenic |
| AAAGTCACCTTCATCAAGAA+TGG | 0.436149 | 5_4:+12636196 | None:intergenic |
| CAGCATATCTGACTGTGTTA+AGG | 0.442168 | 5_4:-12635394 | Msa0838260:CDS |
| AGCATCGCTCACTAAGATAC+TGG | 0.460192 | 5_4:-12636393 | Msa0838260:CDS |
| AGAGGGCGTAAAGGAGTTGA+TGG | 0.467269 | 5_4:-12635498 | Msa0838260:CDS |
| GAGACATTGCCGTTTCTCTC+AGG | 0.473735 | 5_4:-12637641 | Msa0838260:intron |
| GTTTAACTTGGTTGCACCGC+AGG | 0.484247 | 5_4:-12635263 | Msa0838260:CDS |
| AAAAGGATAAACGTTTAACT+TGG | 0.500116 | 5_4:-12635275 | Msa0838260:CDS |
| CGACTACTACAAGTTTCTGT+AGG | 0.502887 | 5_4:+12635525 | None:intergenic |
| GCATGGAAACATATTTGTGA+TGG | 0.508692 | 5_4:-12636308 | Msa0838260:CDS |
| AATTCTGGACAAGGAGCTCT+AGG | 0.508728 | 5_4:+12635633 | None:intergenic |
| TTCTCTGACGCAGCAACGAA+CGG | 0.509181 | 5_4:-12635693 | Msa0838260:CDS |
| AACAGGTCTGAAATGGGATA+AGG | 0.509493 | 5_4:-12636273 | Msa0838260:CDS |
| TTCATCTGAATCCGAGGATG+AGG | 0.517272 | 5_4:-12635586 | Msa0838260:CDS |
| TGCTGTTGTATCCAATAGAT+AGG | 0.518107 | 5_4:-12635238 | Msa0838260:exon |
| AAGGATTTAGATTTAATGCA+AGG | 0.526331 | 5_4:-12635375 | Msa0838260:CDS |
| AAGTCACCTTCATCAAGAAT+GGG | 0.528147 | 5_4:+12636197 | None:intergenic |
| AGTCGCAAAAGAGGGCGTAA+AGG | 0.529428 | 5_4:-12635507 | Msa0838260:CDS |
| CTTTGTTTGGGATGAAGCCA+CGG | 0.540715 | 5_4:-12636177 | Msa0838260:CDS |
| ATTCATTCAATAAGAAAGCA+TGG | 0.542305 | 5_4:-12636325 | Msa0838260:CDS |
| ACTTGTAGTAGTCGCAAAAG+AGG | 0.542854 | 5_4:-12635516 | Msa0838260:CDS |
| TGGTTGACCAAGTACACAAA+GGG | 0.543427 | 5_4:-12636361 | Msa0838260:CDS |
| TAGTAGGTTGAACTGTTTGA+GGG | 0.546289 | 5_4:+12635550 | None:intergenic |
| GTGAATTTACCTGAGAGAAA+CGG | 0.551539 | 5_4:+12637632 | None:intergenic |
| GTGAAGCCCATTCTTGATGA+AGG | 0.552695 | 5_4:-12636203 | Msa0838260:CDS |
| TTTGTTTGGGATGAAGCCAC+GGG | 0.569196 | 5_4:-12636176 | Msa0838260:CDS |
| GTAGTAGGTTGAACTGTTTG+AGG | 0.569252 | 5_4:+12635549 | None:intergenic |
| CTCAGCAGATAGAACAGTCA+AGG | 0.570319 | 5_4:-12636427 | Msa0838260:CDS |
| TGCAGACGCTATATTAGAGA+TGG | 0.575188 | 5_4:-12635469 | Msa0838260:CDS |
| GGCATCTGCTTCAAAGATGA+GGG | 0.578418 | 5_4:-12635448 | Msa0838260:CDS |
| TGGCATCTGCTTCAAAGATG+AGG | 0.587021 | 5_4:-12635449 | Msa0838260:CDS |
| TAGAACAGTCAAGGGCTAAG+TGG | 0.596106 | 5_4:-12636418 | Msa0838260:CDS |
| ATTCGCAATTATAGCTCCCG+TGG | 0.603592 | 5_4:+12636160 | None:intergenic |
| CTTGTAGTAGTCGCAAAAGA+GGG | 0.619141 | 5_4:-12635515 | Msa0838260:CDS |
| GTGCTCAACAATTCTGGACA+AGG | 0.633276 | 5_4:+12635624 | None:intergenic |
| AAAACCGACATTCAGTGTTG+AGG | 0.637621 | 5_4:-12636241 | Msa0838260:CDS |
| TCAGCAGATAGAACAGTCAA+GGG | 0.645530 | 5_4:-12636426 | Msa0838260:CDS |
| TGAGTCTTCATCTGAATCCG+AGG | 0.649200 | 5_4:-12635592 | Msa0838260:CDS |
| ATGGTTGACCAAGTACACAA+AGG | 0.660204 | 5_4:-12636362 | Msa0838260:CDS |
| GTCTCCTCAACACTGAATGT+CGG | 0.665231 | 5_4:+12636237 | None:intergenic |
| GCCGAATTACAAGATCAAGA+AGG | 0.670605 | 5_4:-12636457 | Msa0838260:CDS |
| AATTTGGGAAGAATACATCA+AGG | 0.671593 | 5_4:-12636132 | Msa0838260:intron |
| TGGATACAACAGCACACCTG+CGG | 0.685182 | 5_4:+12635247 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTAAAGAAAAATCAAAAA+TGG | + | chr5_4:12635478-12635497 | None:intergenic | 15.0% |
| !! | AATTCACATACAAAATTTAT+TGG | + | chr5_4:12635582-12635601 | None:intergenic | 15.0% |
| !!! | TATATGAAAAGATATCTTTT+TGG | - | chr5_4:12637581-12637600 | Msa0838260:intron | 15.0% |
| !! | TTGTTAATTGCATATACAAT+GGG | - | chr5_4:12635409-12635428 | Msa0838260:CDS | 20.0% |
| !! | TTTATGTGAGTTTCATTTAT+TGG | - | chr5_4:12635554-12635573 | Msa0838260:CDS | 20.0% |
| !!! | TATAAGATTCTAGTTCATTT+TGG | + | chr5_4:12636166-12636185 | None:intergenic | 20.0% |
| !! | TATGTTAGTTAAACTATAGA+TGG | - | chr5_4:12636305-12636324 | Msa0838260:CDS | 20.0% |
| !! | AATAACAACAAAACAAATGA+TGG | + | chr5_4:12636691-12636710 | None:intergenic | 20.0% |
| !! | ATTAATTGAGTACATCAAAA+TGG | - | chr5_4:12636708-12636727 | Msa0838260:intron | 20.0% |
| !!! | ACTTTTGAATTGTTTCTATT+AGG | - | chr5_4:12636748-12636767 | Msa0838260:intron | 20.0% |
| !!! | ATATTTTTCAACCTATCTAT+TGG | + | chr5_4:12637564-12637583 | None:intergenic | 20.0% |
| ! | TTTCTTCTCTCTTCTTTATT+AGG | - | chr5_4:12635179-12635198 | Msa0838260:three_prime_UTR | 25.0% |
| ! | TTCTTCTCTCTTCTTTATTA+GGG | - | chr5_4:12635180-12635199 | Msa0838260:three_prime_UTR | 25.0% |
| ! | TTCATATGATTCAGAATCAT+GGG | + | chr5_4:12635310-12635329 | None:intergenic | 25.0% |
| ! | GTTGTTAATTGCATATACAA+TGG | - | chr5_4:12635408-12635427 | Msa0838260:CDS | 25.0% |
| ! | AAAATCAAAAATGGAGTACA+TGG | + | chr5_4:12635469-12635488 | None:intergenic | 25.0% |
| ! | TTAAATTGTCTATCCAAACA+GGG | + | chr5_4:12635863-12635882 | None:intergenic | 25.0% |
| ! | ATTAAATTGTCTATCCAAAC+AGG | + | chr5_4:12635864-12635883 | None:intergenic | 25.0% |
| !! | TATGAAATTAGAGACTGTTT+TGG | + | chr5_4:12636093-12636112 | None:intergenic | 25.0% |
| ! | TAAAAATAAGAGGATAACCA+CGG | + | chr5_4:12636213-12636232 | None:intergenic | 25.0% |
| ! | AACATAAGTAATCGATAAAG+AGG | + | chr5_4:12636291-12636310 | None:intergenic | 25.0% |
| ! | ATTCATTCAATAAGAAAGCA+TGG | - | chr5_4:12636463-12636482 | Msa0838260:CDS | 25.0% |
| ! | TAATTGCGAATGATGAAATT+TGG | - | chr5_4:12636640-12636659 | Msa0838260:intron | 25.0% |
| ! | AATTGCGAATGATGAAATTT+GGG | - | chr5_4:12636641-12636660 | Msa0838260:intron | 25.0% |
| ! | GTTATTTATCAGAAGATCAA+CGG | + | chr5_4:12636916-12636935 | None:intergenic | 25.0% |
| ! | AAGGATTTAGATTTAATGCA+AGG | - | chr5_4:12637413-12637432 | Msa0838260:intron | 25.0% |
| ! | AAAAATATCTCTCTTGCATT+AGG | + | chr5_4:12637485-12637504 | None:intergenic | 25.0% |
| !!! | GATATTTTTGTCTCTCAAAA+AGG | - | chr5_4:12637496-12637515 | Msa0838260:intron | 25.0% |
| ! | AAAAGGATAAACGTTTAACT+TGG | - | chr5_4:12637513-12637532 | Msa0838260:intron | 25.0% |
| TCAATTTAAGCATTCTACGT+TGG | + | chr5_4:12635274-12635293 | None:intergenic | 30.0% | |
| CTTCATATGATTCAGAATCA+TGG | + | chr5_4:12635311-12635330 | None:intergenic | 30.0% | |
| !!! | TTGAGATTTTGATTCTTCAC+TGG | - | chr5_4:12635374-12635393 | Msa0838260:CDS | 30.0% |
| AAAATCAAATCTCCAACTGA+AGG | + | chr5_4:12635617-12635636 | None:intergenic | 30.0% | |
| ATGTTCTTTCATTTCTCACA+TGG | - | chr5_4:12635643-12635662 | Msa0838260:CDS | 30.0% | |
| AATGAGCTGAAAGCAATTTA+TGG | - | chr5_4:12635985-12636004 | Msa0838260:intron | 30.0% | |
| AGAATCTTATATCCAAGTAC+TGG | - | chr5_4:12636175-12636194 | Msa0838260:CDS | 30.0% | |
| TGTGCATGCATAAAAATAAG+AGG | + | chr5_4:12636223-12636242 | None:intergenic | 30.0% | |
| ! | GATGGATTTCACAAAAAAAC+AGG | - | chr5_4:12636498-12636517 | Msa0838260:intron | 30.0% |
| ACAAAAAAACAGGTCTGAAA+TGG | - | chr5_4:12636508-12636527 | Msa0838260:intron | 30.0% | |
| ! | CAAAAAAACAGGTCTGAAAT+GGG | - | chr5_4:12636509-12636528 | Msa0838260:intron | 30.0% |
| AATTTGGGAAGAATACATCA+AGG | - | chr5_4:12636656-12636675 | Msa0838260:intron | 30.0% | |
| ! | ACAACAAAACAAATGATGGA+TGG | + | chr5_4:12636687-12636706 | None:intergenic | 30.0% |
| TACAATGGAAAGGAAAGATT+GGG | + | chr5_4:12636946-12636965 | None:intergenic | 30.0% | |
| ATACAATGGAAAGGAAAGAT+TGG | + | chr5_4:12636947-12636966 | None:intergenic | 30.0% | |
| TAGATTGTGCTCAAATACAA+TGG | + | chr5_4:12636961-12636980 | None:intergenic | 30.0% | |
| ! | TTTAGAGTTATGTTGCTCTA+TGG | + | chr5_4:12637372-12637391 | None:intergenic | 30.0% |
| GTGAATTTACCTGAGAGAAA+CGG | + | chr5_4:12635159-12635178 | None:intergenic | 35.0% | |
| ATTTAAGCATTCTACGTTGG+TGG | + | chr5_4:12635271-12635290 | None:intergenic | 35.0% | |
| ! | TTTTTGCCCTTTTCCATTGA+TGG | - | chr5_4:12635337-12635356 | Msa0838260:CDS | 35.0% |
| ! | TTTTGCCCTTTTCCATTGAT+GGG | - | chr5_4:12635338-12635357 | Msa0838260:CDS | 35.0% |
| GCAAAATGAGCCTAATGTTA+GGG | + | chr5_4:12635444-12635463 | None:intergenic | 35.0% | |
| TGCAAAATGAGCCTAATGTT+AGG | + | chr5_4:12635445-12635464 | None:intergenic | 35.0% | |
| AGTACCATATATCCTTCAGT+TGG | - | chr5_4:12635602-12635621 | Msa0838260:CDS | 35.0% | |
| ATCTCCAACTGAAGGATATA+TGG | + | chr5_4:12635609-12635628 | None:intergenic | 35.0% | |
| GAATGCGCAAACAAAAGAAA+AGG | + | chr5_4:12635706-12635725 | None:intergenic | 35.0% | |
| GTTTGCGCATTCAAATATCT+AGG | - | chr5_4:12635714-12635733 | Msa0838260:CDS | 35.0% | |
| CTCATTTCTATAAGCACTCT+AGG | + | chr5_4:12635971-12635990 | None:intergenic | 35.0% | |
| GCCTTCTTGATCTTGTAATT+CGG | + | chr5_4:12636335-12636354 | None:intergenic | 35.0% | |
| TAAGATACTGGCAGATTTGA+TGG | - | chr5_4:12636407-12636426 | Msa0838260:CDS | 35.0% | |
| GTTTATTCCCTTTGTGTACT+TGG | + | chr5_4:12636437-12636456 | None:intergenic | 35.0% | |
| GCATGGAAACATATTTGTGA+TGG | - | chr5_4:12636480-12636499 | Msa0838260:intron | 35.0% | |
| AAGTCACCTTCATCAAGAAT+GGG | + | chr5_4:12636594-12636613 | None:intergenic | 35.0% | |
| AAAGTCACCTTCATCAAGAA+TGG | + | chr5_4:12636595-12636614 | None:intergenic | 35.0% | |
| TTGATGAAGGTGACTTTGTT+TGG | - | chr5_4:12636598-12636617 | Msa0838260:intron | 35.0% | |
| ! | TGATGAAGGTGACTTTGTTT+GGG | - | chr5_4:12636599-12636618 | Msa0838260:intron | 35.0% |
| TTTACCCCTCTTCTATAGTA+AGG | - | chr5_4:12636772-12636791 | Msa0838260:intron | 35.0% | |
| ACAATGGAAAGGAAAGATTG+GGG | + | chr5_4:12636945-12636964 | None:intergenic | 35.0% | |
| TGTGCTCAAATACAATGGAA+AGG | + | chr5_4:12636956-12636975 | None:intergenic | 35.0% | |
| GTACATAGCTCCTTAAAGAT+TGG | + | chr5_4:12637071-12637090 | None:intergenic | 35.0% | |
| ! | TAGTAGGTTGAACTGTTTGA+GGG | + | chr5_4:12637241-12637260 | None:intergenic | 35.0% |
| TGCTGTTGTATCCAATAGAT+AGG | - | chr5_4:12637550-12637569 | Msa0838260:intron | 35.0% | |
| ! | TTTGCCCTTTTCCATTGATG+GGG | - | chr5_4:12635339-12635358 | Msa0838260:CDS | 40.0% |
| GAAACCCCATCAATGGAAAA+GGG | + | chr5_4:12635346-12635365 | None:intergenic | 40.0% | |
| AGAAACCCCATCAATGGAAA+AGG | + | chr5_4:12635347-12635366 | None:intergenic | 40.0% | |
| AATCAGAGAAACCCCATCAA+TGG | + | chr5_4:12635353-12635372 | None:intergenic | 40.0% | |
| GCTATCACTACCCTAACATT+AGG | - | chr5_4:12635431-12635450 | Msa0838260:CDS | 40.0% | |
| CTGTGATTCATCATGGTCTA+AGG | - | chr5_4:12635837-12635856 | Msa0838260:intron | 40.0% | |
| AGACTGTTTGTGAGAGCTTA+TGG | + | chr5_4:12636029-12636048 | None:intergenic | 40.0% | |
| CAAGAGTGAATTCCAGTACT+TGG | + | chr5_4:12636190-12636209 | None:intergenic | 40.0% | |
| GCCGAATTACAAGATCAAGA+AGG | - | chr5_4:12636331-12636350 | Msa0838260:CDS | 40.0% | |
| TCAGCAGATAGAACAGTCAA+GGG | - | chr5_4:12636362-12636381 | Msa0838260:CDS | 40.0% | |
| ATGGTTGACCAAGTACACAA+AGG | - | chr5_4:12636426-12636445 | Msa0838260:CDS | 40.0% | |
| TGGTTGACCAAGTACACAAA+GGG | - | chr5_4:12636427-12636446 | Msa0838260:CDS | 40.0% | |
| ! | AACAGGTCTGAAATGGGATA+AGG | - | chr5_4:12636515-12636534 | Msa0838260:intron | 40.0% |
| AAAACCGACATTCAGTGTTG+AGG | - | chr5_4:12636547-12636566 | Msa0838260:intron | 40.0% | |
| CAAAAGTTTCCTCGTGAAGA+AGG | + | chr5_4:12636735-12636754 | None:intergenic | 40.0% | |
| AGCTCCTTACTATAGAAGAG+GGG | + | chr5_4:12636779-12636798 | None:intergenic | 40.0% | |
| GAGCTCCTTACTATAGAAGA+GGG | + | chr5_4:12636780-12636799 | None:intergenic | 40.0% | |
| AGAGCTCCTTACTATAGAAG+AGG | + | chr5_4:12636781-12636800 | None:intergenic | 40.0% | |
| TCTACCTAAGAGGGAAAAGT+GGG | - | chr5_4:12636798-12636817 | Msa0838260:intron | 40.0% | |
| TGATGTGTGCTCAACAATTC+TGG | + | chr5_4:12637173-12637192 | None:intergenic | 40.0% | |
| ATTTGTCTCTTCCTCATCCT+CGG | + | chr5_4:12637216-12637235 | None:intergenic | 40.0% | |
| ! | GTAGTAGGTTGAACTGTTTG+AGG | + | chr5_4:12637242-12637261 | None:intergenic | 40.0% |
| CAAGTTTCTGTAGGTGTAGT+AGG | + | chr5_4:12637257-12637276 | None:intergenic | 40.0% | |
| CGACTACTACAAGTTTCTGT+AGG | + | chr5_4:12637266-12637285 | None:intergenic | 40.0% | |
| ACTTGTAGTAGTCGCAAAAG+AGG | - | chr5_4:12637272-12637291 | Msa0838260:intron | 40.0% | |
| CTTGTAGTAGTCGCAAAAGA+GGG | - | chr5_4:12637273-12637292 | Msa0838260:intron | 40.0% | |
| TGCAGACGCTATATTAGAGA+TGG | - | chr5_4:12637319-12637338 | Msa0838260:intron | 40.0% | |
| CAGCATATCTGACTGTGTTA+AGG | - | chr5_4:12637394-12637413 | Msa0838260:intron | 40.0% | |
| CACAAAAATCCCTCCACAAC+AGG | + | chr5_4:12635760-12635779 | None:intergenic | 45.0% | |
| ! | GGGATTTTTGTGCTCTTCTG+TGG | - | chr5_4:12635768-12635787 | Msa0838260:intron | 45.0% |
| ACCATGATGAATCACAGCGA+GGG | + | chr5_4:12635834-12635853 | None:intergenic | 45.0% | |
| CTCAGCAGATAGAACAGTCA+AGG | - | chr5_4:12636361-12636380 | Msa0838260:CDS | 45.0% | |
| TAGAACAGTCAAGGGCTAAG+TGG | - | chr5_4:12636370-12636389 | Msa0838260:CDS | 45.0% | |
| AGCATCGCTCACTAAGATAC+TGG | - | chr5_4:12636395-12636414 | Msa0838260:CDS | 45.0% | |
| GTCTCCTCAACACTGAATGT+CGG | + | chr5_4:12636554-12636573 | None:intergenic | 45.0% | |
| GTGAAGCCCATTCTTGATGA+AGG | - | chr5_4:12636585-12636604 | Msa0838260:intron | 45.0% | |
| ! | CTTTGTTTGGGATGAAGCCA+CGG | - | chr5_4:12636611-12636630 | Msa0838260:intron | 45.0% |
| ! | TTTGTTTGGGATGAAGCCAC+GGG | - | chr5_4:12636612-12636631 | Msa0838260:intron | 45.0% |
| ATTCGCAATTATAGCTCCCG+TGG | + | chr5_4:12636631-12636650 | None:intergenic | 45.0% | |
| CAAAATGGTCCTTCTTCACG+AGG | - | chr5_4:12636723-12636742 | Msa0838260:intron | 45.0% | |
| AGTAAGGAGCTCTACCTAAG+AGG | - | chr5_4:12636788-12636807 | Msa0838260:intron | 45.0% | |
| GTAAGGAGCTCTACCTAAGA+GGG | - | chr5_4:12636789-12636808 | Msa0838260:intron | 45.0% | |
| CTCTACCTAAGAGGGAAAAG+TGG | - | chr5_4:12636797-12636816 | Msa0838260:intron | 45.0% | |
| CTACCTAAGAGGGAAAAGTG+GGG | - | chr5_4:12636799-12636818 | Msa0838260:intron | 45.0% | |
| !!! | GCCACCACTTTTTACAGTCT+CGG | + | chr5_4:12637045-12637064 | None:intergenic | 45.0% |
| TGGTGGCTGTCCAATCTTTA+AGG | - | chr5_4:12637058-12637077 | Msa0838260:intron | 45.0% | |
| AATTCTGGACAAGGAGCTCT+AGG | + | chr5_4:12637158-12637177 | None:intergenic | 45.0% | |
| GTGCTCAACAATTCTGGACA+AGG | + | chr5_4:12637167-12637186 | None:intergenic | 45.0% | |
| TGAGTCTTCATCTGAATCCG+AGG | - | chr5_4:12637196-12637215 | Msa0838260:intron | 45.0% | |
| TTCATCTGAATCCGAGGATG+AGG | - | chr5_4:12637202-12637221 | Msa0838260:intron | 45.0% | |
| TGGCATCTGCTTCAAAGATG+AGG | - | chr5_4:12637339-12637358 | Msa0838260:intron | 45.0% | |
| GGCATCTGCTTCAAAGATGA+GGG | - | chr5_4:12637340-12637359 | Msa0838260:intron | 45.0% | |
| ! | GAGACATTGCCGTTTCTCTC+AGG | - | chr5_4:12635147-12635166 | Msa0838260:three_prime_UTR | 50.0% |
| AGTTGTGAGTCCTGTTGTGG+AGG | - | chr5_4:12635747-12635766 | Msa0838260:CDS | 50.0% | |
| GTTGTGAGTCCTGTTGTGGA+GGG | - | chr5_4:12635748-12635767 | Msa0838260:CDS | 50.0% | |
| GACCATGATGAATCACAGCG+AGG | + | chr5_4:12635835-12635854 | None:intergenic | 50.0% | |
| ! | TCATGGTCTAAGGCCCTGTT+TGG | - | chr5_4:12635847-12635866 | Msa0838260:intron | 50.0% |
| ACTGGAATTCACTCTTGCCG+TGG | - | chr5_4:12636193-12636212 | Msa0838260:CDS | 50.0% | |
| !! | ACTCCCCACTTTTCCCTCTT+AGG | + | chr5_4:12636805-12636824 | None:intergenic | 50.0% |
| ! | CTTTTTACAGTCTCGGCGTC+AGG | + | chr5_4:12637038-12637057 | None:intergenic | 50.0% |
| GACGCCGAGACTGTAAAAAG+TGG | - | chr5_4:12637038-12637057 | Msa0838260:intron | 50.0% | |
| GCCGAGACTGTAAAAAGTGG+TGG | - | chr5_4:12637041-12637060 | Msa0838260:intron | 50.0% | |
| TTCTCTGACGCAGCAACGAA+CGG | - | chr5_4:12637095-12637114 | Msa0838260:intron | 50.0% | |
| ! | AGTCGCAAAAGAGGGCGTAA+AGG | - | chr5_4:12637281-12637300 | Msa0838260:intron | 50.0% |
| ! | AGAGGGCGTAAAGGAGTTGA+TGG | - | chr5_4:12637290-12637309 | Msa0838260:intron | 50.0% |
| !! | GTTTAACTTGGTTGCACCGC+AGG | - | chr5_4:12637525-12637544 | Msa0838260:intron | 50.0% |
| TGGATACAACAGCACACCTG+CGG | + | chr5_4:12637544-12637563 | None:intergenic | 50.0% | |
| GCGAGTTGTGAGTCCTGTTG+TGG | - | chr5_4:12635744-12635763 | Msa0838260:CDS | 55.0% | |
| GCCCTCGCTGTGATTCATCA+TGG | - | chr5_4:12635830-12635849 | Msa0838260:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 12635133 | 12637677 | 12635133 | ID=Msa0838260;Name=Msa0838260 |
| chr5_4 | mRNA | 12635133 | 12637677 | 12635133 | ID=Msa0838260-mRNA-1;Parent=Msa0838260;Name=Msa0838260-mRNA-1;_AED=0.03;_eAED=0.03;_QI=36|1|1|1|0.5|0.66|3|106|298 |
| chr5_4 | exon | 12635133 | 12635781 | 12635133 | ID=Msa0838260-mRNA-1:exon:5575;Parent=Msa0838260-mRNA-1 |
| chr5_4 | exon | 12636133 | 12636486 | 12636133 | ID=Msa0838260-mRNA-1:exon:5574;Parent=Msa0838260-mRNA-1 |
| chr5_4 | exon | 12637642 | 12637677 | 12637642 | ID=Msa0838260-mRNA-1:exon:5573;Parent=Msa0838260-mRNA-1 |
| chr5_4 | five_prime_UTR | 12637642 | 12637677 | 12637642 | ID=Msa0838260-mRNA-1:five_prime_utr;Parent=Msa0838260-mRNA-1 |
| chr5_4 | CDS | 12636133 | 12636486 | 12636133 | ID=Msa0838260-mRNA-1:cds;Parent=Msa0838260-mRNA-1 |
| chr5_4 | CDS | 12635239 | 12635781 | 12635239 | ID=Msa0838260-mRNA-1:cds;Parent=Msa0838260-mRNA-1 |
| chr5_4 | three_prime_UTR | 12635133 | 12635238 | 12635133 | ID=Msa0838260-mRNA-1:three_prime_utr;Parent=Msa0838260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
