AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0843250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843250 MtrunA17_Chr5g0413951 91.870 123 10 0 1 123 1 123 6.03e-74 224
Msa0843250 MtrunA17_Chr5g0413941 83.333 120 20 0 1 120 1 120 1.28e-66 205
Msa0843250 MtrunA17_Chr5g0413931 74.380 121 29 1 3 123 4 122 1.74e-57 182
Msa0843250 MtrunA17_Chr5g0413921 67.241 116 38 0 5 120 5 120 3.77e-50 163
Msa0843250 MtrunA17_Chr7g0227921 45.872 109 59 0 12 120 16 124 1.59e-23 93.6
Msa0843250 MtrunA17_Chr6g0451181 43.119 109 62 0 12 120 17 125 5.71e-23 87.4
Msa0843250 MtrunA17_Chr6g0451261 41.441 111 65 0 10 120 14 124 4.24e-22 90.9
Msa0843250 MtrunA17_Chr6g0451251 47.273 110 57 1 12 120 17 126 8.51e-22 89.0
Msa0843250 MtrunA17_Chr6g0451211 41.441 111 63 1 12 120 17 127 9.70e-21 86.3
Msa0843250 MtrunA17_Chr6g0451171 42.708 96 55 0 25 120 30 125 1.20e-20 85.9
Msa0843250 MtrunA17_Chr6g0451201 44.954 109 58 1 12 120 17 123 8.90e-20 83.6
Msa0843250 MtrunA17_Chr3g0126101 43.119 109 61 1 12 120 11 118 2.15e-18 79.7
Msa0843250 MtrunA17_Chr5g0413961 38.532 109 66 1 12 120 19 126 1.34e-17 77.4
Msa0843250 MtrunA17_Chr1g0150151 39.286 112 67 1 10 120 14 125 3.42e-16 73.6
Msa0843250 MtrunA17_Chr4g0059311 40.541 111 63 3 12 120 16 125 3.94e-15 70.5
Msa0843250 MtrunA17_Chr3g0126091 39.474 114 66 3 12 122 16 129 9.46e-14 66.6
Msa0843250 MtrunA17_Chr1g0155761 37.273 110 67 2 12 120 26 134 3.29e-13 65.1
Msa0843250 MtrunA17_Chr3g0114731 38.182 110 66 2 12 120 23 131 5.77e-13 64.7
Msa0843250 MtrunA17_Chr1g0182291 36.364 99 61 2 23 120 31 128 1.32e-11 60.5
Msa0843250 MtrunA17_Chr5g0408031 31.481 108 72 2 12 118 15 121 2.61e-11 59.7
Msa0843250 MtrunA17_Chr6g0463971 34.259 108 68 2 12 118 21 126 5.02e-11 58.9
Msa0843250 MtrunA17_Chr5g0408001 32.727 110 72 2 12 120 15 123 5.70e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843250 AT5G40210.1 43.363 113 64 0 8 120 13 125 6.44e-24 94.7
Msa0843250 AT3G28050.1 45.045 111 61 0 10 120 14 124 9.48e-20 84.0
Msa0843250 AT5G40230.1 42.342 111 64 0 10 120 23 133 1.01e-19 84.0
Msa0843250 AT5G40240.2 40.367 109 65 0 12 120 38 146 9.19e-19 81.3
Msa0843250 AT5G40240.1 40.367 109 65 0 12 120 24 132 9.56e-19 81.3
Msa0843250 AT5G40240.3 40.367 109 65 0 12 120 24 132 9.56e-19 81.3
Msa0843250 AT3G28100.3 46.847 111 59 0 10 120 16 126 3.95e-17 75.5
Msa0843250 AT3G28100.2 46.847 111 59 0 10 120 16 126 5.44e-17 75.1
Msa0843250 AT3G28100.1 46.847 111 59 0 10 120 16 126 5.76e-17 75.9
Msa0843250 AT4G15540.1 42.342 111 64 0 10 120 19 129 1.67e-16 74.7
Msa0843250 AT4G15540.2 42.342 111 64 0 10 120 19 129 1.67e-16 74.7
Msa0843250 AT3G28080.3 46.847 111 59 0 10 120 16 126 2.37e-16 73.9
Msa0843250 AT3G28080.1 46.847 111 59 0 10 120 16 126 4.70e-16 73.6
Msa0843250 AT3G28130.6 43.243 111 63 0 10 120 15 125 2.86e-15 69.7
Msa0843250 AT3G28130.4 42.609 115 66 0 6 120 11 125 4.91e-15 70.1
Msa0843250 AT3G28130.2 42.609 115 66 0 6 120 11 125 5.49e-15 70.5
Msa0843250 AT5G07050.1 34.545 110 70 2 12 120 22 130 2.01e-12 63.5
Msa0843250 AT5G45370.2 32.727 110 72 2 10 118 22 130 4.68e-11 59.3
Msa0843250 AT5G45370.4 32.727 110 72 2 10 118 22 130 4.68e-11 59.3
Msa0843250 AT5G45370.1 32.727 110 72 2 10 118 22 130 4.99e-11 59.3
Msa0843250 AT3G28070.3 45.946 111 60 0 10 120 19 129 9.10e-11 58.5

Find 23 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATTGATGTTGAAAT+TGG 0.226936 5_4:-20884602 None:intergenic
TTCTGCAGATTACTTCTTCT+TGG 0.233740 5_4:+20884620 Msa0843250:CDS
ACTCATATGTTAATGACTTC+TGG 0.288657 5_4:+20884755 Msa0843250:CDS
ATAGCAAGTATGAAGGTAAA+AGG 0.300566 5_4:-20884830 None:intergenic
AAATGTCAAAATTGTTAGAT+TGG 0.329958 5_4:-20884272 None:intergenic
TACTTCTTCTTGGTTGCCTC+AGG 0.379746 5_4:+20884630 Msa0843250:CDS
TCATACTTGCTATAATTTCA+AGG 0.384041 5_4:+20884840 Msa0843250:CDS
ATGAAAGCAGCCACAAAAGA+TGG 0.452927 5_4:+20884482 Msa0843250:CDS
AAAATTGATGTTGAAATTGG+AGG 0.500522 5_4:-20884599 None:intergenic
CTATGGTGGAAGAGAGTGGA+TGG 0.506366 5_4:-20884563 None:intergenic
TGAAATTATAGCAAGTATGA+AGG 0.526932 5_4:-20884837 None:intergenic
AACAATAGCTGAAGCCAACA+TGG 0.534674 5_4:-20884795 None:intergenic
GAAGAAAACATAGAGCCAAA+AGG 0.540600 5_4:-20884538 None:intergenic
ATTGATGTTGAAATTGGAGG+AGG 0.546987 5_4:-20884596 None:intergenic
TAAATTCAGTTCTCCCATGT+TGG 0.547322 5_4:+20884781 Msa0843250:CDS
GTGAGAATTAACAAGCAAGT+TGG 0.550219 5_4:+20884357 Msa0843250:five_prime_UTR
TGCAGCTCAAATTGTTGAAG+TGG 0.587755 5_4:+20884445 Msa0843250:CDS
GCAAGTATGAAGGTAAAAGG+AGG 0.605835 5_4:-20884827 None:intergenic
CAAGTATGAAGGTAAAAGGA+GGG 0.622141 5_4:-20884826 None:intergenic
GCAGCTCAAATTGTTGAAGT+GGG 0.628887 5_4:+20884446 Msa0843250:CDS
ACAATAGCTGAAGCCAACAT+GGG 0.645656 5_4:-20884794 None:intergenic
TCGGACTAAAGTTAAAGTTG+AGG 0.649103 5_4:+20884302 Msa0843250:five_prime_UTR
GCTCAAATTGTTGAAGTGGG+TGG 0.711164 5_4:+20884449 Msa0843250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATAAAGATTGAATT+TGG + chr5_4:20884693-20884712 Msa0843250:intron 10.0%
!!! ATTTTGACATTTTTATTAGT+CGG + chr5_4:20884283-20884302 Msa0843250:five_prime_UTR 15.0%
!! AAATGTCAAAATTGTTAGAT+TGG - chr5_4:20884275-20884294 None:intergenic 20.0%
!!! GGTATGTTGTTTTTATTTTA+TGG + chr5_4:20884861-20884880 Msa0843250:CDS 20.0%
!!! ATTTTATGGTTGAAATTACA+AGG + chr5_4:20884875-20884894 Msa0843250:CDS 20.0%
!!! CGTCTTACTTTTATAATATC+TGG - chr5_4:20884338-20884357 None:intergenic 25.0%
!! CATTGTTTACAAAAACCTTT+TGG + chr5_4:20884523-20884542 Msa0843250:CDS 25.0%
! AAAATTGATGTTGAAATTGG+AGG - chr5_4:20884602-20884621 None:intergenic 25.0%
! CAGAAAATTGATGTTGAAAT+TGG - chr5_4:20884605-20884624 None:intergenic 25.0%
! TGAAATTATAGCAAGTATGA+AGG - chr5_4:20884840-20884859 None:intergenic 25.0%
!!! TCATACTTGCTATAATTTCA+AGG + chr5_4:20884840-20884859 Msa0843250:CDS 25.0%
!!! TACAAGGTTTTGTTGTTTTT+TGG + chr5_4:20884891-20884910 Msa0843250:CDS 25.0%
! AATACTCATTCCATCTTTTG+TGG - chr5_4:20884495-20884514 None:intergenic 30.0%
! TACTATTTTGTTCATACCTG+AGG - chr5_4:20884649-20884668 None:intergenic 30.0%
TTTGGCAAAATAGAGTGATA+TGG + chr5_4:20884711-20884730 Msa0843250:intron 30.0%
ACTCATATGTTAATGACTTC+TGG + chr5_4:20884755-20884774 Msa0843250:CDS 30.0%
ATAGCAAGTATGAAGGTAAA+AGG - chr5_4:20884833-20884852 None:intergenic 30.0%
TCGGACTAAAGTTAAAGTTG+AGG + chr5_4:20884302-20884321 Msa0843250:five_prime_UTR 35.0%
GTGAGAATTAACAAGCAAGT+TGG + chr5_4:20884357-20884376 Msa0843250:five_prime_UTR 35.0%
CAGACAGAGACAAGAAAAAA+TGG + chr5_4:20884388-20884407 Msa0843250:exon 35.0%
GAAGAAAACATAGAGCCAAA+AGG - chr5_4:20884541-20884560 None:intergenic 35.0%
ATTGATGTTGAAATTGGAGG+AGG - chr5_4:20884599-20884618 None:intergenic 35.0%
TTCTGCAGATTACTTCTTCT+TGG + chr5_4:20884620-20884639 Msa0843250:CDS 35.0%
TAAATTCAGTTCTCCCATGT+TGG + chr5_4:20884781-20884800 Msa0843250:CDS 35.0%
CAAGTATGAAGGTAAAAGGA+GGG - chr5_4:20884829-20884848 None:intergenic 35.0%
AGACAAGAAAAAATGGCGAG+TGG + chr5_4:20884395-20884414 Msa0843250:exon 40.0%
TGCAGCTCAAATTGTTGAAG+TGG + chr5_4:20884445-20884464 Msa0843250:CDS 40.0%
GCAGCTCAAATTGTTGAAGT+GGG + chr5_4:20884446-20884465 Msa0843250:CDS 40.0%
ATGAAAGCAGCCACAAAAGA+TGG + chr5_4:20884482-20884501 Msa0843250:CDS 40.0%
!! TTTTCTATGGTGGAAGAGAG+TGG - chr5_4:20884570-20884589 None:intergenic 40.0%
ACAATAGCTGAAGCCAACAT+GGG - chr5_4:20884797-20884816 None:intergenic 40.0%
AACAATAGCTGAAGCCAACA+TGG - chr5_4:20884798-20884817 None:intergenic 40.0%
GCAAGTATGAAGGTAAAAGG+AGG - chr5_4:20884830-20884849 None:intergenic 40.0%
CAAGAAAAAATGGCGAGTGG+AGG + chr5_4:20884398-20884417 Msa0843250:exon 45.0%
GCTCAAATTGTTGAAGTGGG+TGG + chr5_4:20884449-20884468 Msa0843250:CDS 45.0%
! GAGGAGCTCTTTTTCTATGG+TGG - chr5_4:20884580-20884599 None:intergenic 45.0%
! GAGGAGGAGCTCTTTTTCTA+TGG - chr5_4:20884583-20884602 None:intergenic 45.0%
TACTTCTTCTTGGTTGCCTC+AGG + chr5_4:20884630-20884649 Msa0843250:CDS 45.0%
! CTATGGTGGAAGAGAGTGGA+TGG - chr5_4:20884566-20884585 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 20884271 20884919 20884271 ID=Msa0843250;Name=Msa0843250
chr5_4 mRNA 20884271 20884919 20884271 ID=Msa0843250-mRNA-1;Parent=Msa0843250;Name=Msa0843250-mRNA-1;_AED=0.38;_eAED=0.38;_QI=136|1|0.5|1|1|1|2|0|139
chr5_4 exon 20884271 20884651 20884271 ID=Msa0843250-mRNA-1:exon:8680;Parent=Msa0843250-mRNA-1
chr5_4 exon 20884748 20884919 20884748 ID=Msa0843250-mRNA-1:exon:8681;Parent=Msa0843250-mRNA-1
chr5_4 five_prime_UTR 20884271 20884406 20884271 ID=Msa0843250-mRNA-1:five_prime_utr;Parent=Msa0843250-mRNA-1
chr5_4 CDS 20884407 20884651 20884407 ID=Msa0843250-mRNA-1:cds;Parent=Msa0843250-mRNA-1
chr5_4 CDS 20884748 20884919 20884748 ID=Msa0843250-mRNA-1:cds;Parent=Msa0843250-mRNA-1
Gene Sequence

>Msa0843250

ATGGCGAGTGGAGGAGCAAGTGTAACAACAACTATGATTGCAGCTCAAATTGTTGAAGTGGGTGGTGAAACTCTAATGAAAGCAGCCACAAAAGATGGAATGAGTATTTTTATTTTCATTGTTTACAAAAACCTTTTGGCTCTATGTTTTCTTCTACCATCCACTCTCTTCCACCATAGAAAAAGAGCTCCTCCTCCAATTTCAACATCAATTTTCTGCAGATTACTTCTTCTTGGTTGCCTCAGAACTGCAACTCATATGTTAATGACTTCTGGAATTAAATTCAGTTCTCCCATGTTGGCTTCAGCTATTGTTAATCTTGTCCCTCCTTTTACCTTCATACTTGCTATAATTTCAAGGTATGTTGTTTTTATTTTATGGTTGAAATTACAAGGTTTTGTTGTTTTTTGGATTAGA

Protein sequence

>Msa0843250

MASGGASVTTTMIAAQIVEVGGETLMKAATKDGMSIFIFIVYKNLLALCFLLPSTLFHHRKRAPPPISTSIFCRLLLLGCLRTATHMLMTSGIKFSSPMLASAIVNLVPPFTFILAIISRYVVFILWLKLQGFVVFWIR