AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0723110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723110 MtrunA17_Chr5g0413951 93.333 120 8 0 1 120 1 120 2.51e-74 224
Msa0723110 MtrunA17_Chr5g0413941 83.333 120 20 0 1 120 1 120 5.50e-67 206
Msa0723110 MtrunA17_Chr5g0413931 75.424 118 27 1 3 120 4 119 8.52e-58 182
Msa0723110 MtrunA17_Chr5g0413921 67.241 116 38 0 5 120 5 120 1.36e-50 164
Msa0723110 MtrunA17_Chr7g0227921 45.872 109 59 0 12 120 16 124 1.16e-23 93.6
Msa0723110 MtrunA17_Chr6g0451181 43.119 109 62 0 12 120 17 125 3.51e-23 87.8
Msa0723110 MtrunA17_Chr6g0451261 41.441 111 65 0 10 120 14 124 2.39e-22 91.3
Msa0723110 MtrunA17_Chr6g0451251 52.041 98 46 1 24 120 29 126 7.03e-22 89.0
Msa0723110 MtrunA17_Chr6g0451211 41.441 111 63 1 12 120 17 127 7.56e-21 86.3
Msa0723110 MtrunA17_Chr6g0451171 42.708 96 55 0 25 120 30 125 7.93e-21 85.9
Msa0723110 MtrunA17_Chr6g0451201 44.954 109 58 1 12 120 17 123 5.57e-20 83.6
Msa0723110 MtrunA17_Chr3g0126101 43.119 109 61 1 12 120 11 118 1.19e-18 80.1
Msa0723110 MtrunA17_Chr5g0413961 38.532 109 66 1 12 120 19 126 8.70e-18 77.8
Msa0723110 MtrunA17_Chr1g0150151 39.286 112 67 1 10 120 14 125 1.76e-16 73.9
Msa0723110 MtrunA17_Chr4g0059311 40.541 111 63 3 12 120 16 125 2.62e-15 70.9
Msa0723110 MtrunA17_Chr3g0126091 39.474 114 66 3 12 122 16 129 8.33e-14 66.6
Msa0723110 MtrunA17_Chr1g0155761 37.273 110 67 2 12 120 26 134 1.98e-13 65.5
Msa0723110 MtrunA17_Chr3g0114731 38.182 110 66 2 12 120 23 131 2.70e-13 65.1
Msa0723110 MtrunA17_Chr1g0182291 36.364 99 61 2 23 120 31 128 9.54e-12 60.8
Msa0723110 MtrunA17_Chr5g0408031 31.481 108 72 2 12 118 15 121 1.37e-11 60.5
Msa0723110 MtrunA17_Chr5g0408001 32.727 110 72 2 12 120 15 123 2.67e-11 59.3
Msa0723110 MtrunA17_Chr6g0463971 34.259 108 68 2 12 118 21 126 4.47e-11 58.9
Msa0723110 MtrunA17_Chr3g0129601 37.113 97 59 2 25 120 47 142 5.40e-11 58.5
Msa0723110 MtrunA17_Chr8g0369841 32.727 110 72 2 10 118 12 120 8.52e-11 58.2
Msa0723110 MtrunA17_Chr3g0114711 34.862 109 69 2 13 120 20 127 8.55e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723110 AT5G40210.1 43.363 113 64 0 8 120 13 125 3.26e-24 95.1
Msa0723110 AT5G40230.1 42.342 111 64 0 10 120 23 133 5.26e-20 84.3
Msa0723110 AT3G28050.1 45.045 111 61 0 10 120 14 124 6.24e-20 84.0
Msa0723110 AT5G40240.1 40.367 109 65 0 12 120 24 132 4.81e-19 81.6
Msa0723110 AT5G40240.3 40.367 109 65 0 12 120 24 132 4.81e-19 81.6
Msa0723110 AT5G40240.2 40.367 109 65 0 12 120 38 146 5.12e-19 81.6
Msa0723110 AT3G28100.3 46.847 111 59 0 10 120 16 126 2.62e-17 75.9
Msa0723110 AT3G28100.2 46.847 111 59 0 10 120 16 126 3.43e-17 75.1
Msa0723110 AT3G28100.1 46.847 111 59 0 10 120 16 126 5.17e-17 75.9
Msa0723110 AT4G15540.1 42.342 111 64 0 10 120 19 129 1.08e-16 75.1
Msa0723110 AT4G15540.2 42.342 111 64 0 10 120 19 129 1.08e-16 75.1
Msa0723110 AT3G28080.3 46.847 111 59 0 10 120 16 126 2.12e-16 73.9
Msa0723110 AT3G28080.1 46.847 111 59 0 10 120 16 126 4.03e-16 73.6
Msa0723110 AT3G28130.6 42.609 115 66 0 6 120 11 125 1.67e-15 70.1
Msa0723110 AT3G28130.4 42.609 115 66 0 6 120 11 125 3.36e-15 70.1
Msa0723110 AT3G28130.2 42.609 115 66 0 6 120 11 125 3.83e-15 70.9
Msa0723110 AT5G07050.1 34.545 110 70 2 12 120 22 130 1.26e-12 63.5
Msa0723110 AT5G45370.2 32.727 110 72 2 10 118 22 130 2.88e-11 59.7
Msa0723110 AT5G45370.4 32.727 110 72 2 10 118 22 130 2.88e-11 59.7
Msa0723110 AT5G45370.1 32.727 110 72 2 10 118 22 130 3.04e-11 59.7
Msa0723110 AT2G39510.1 34.234 111 71 2 11 120 11 120 5.32e-11 58.9
Msa0723110 AT3G28070.1 45.946 111 60 0 10 120 19 129 6.04e-11 58.9
Msa0723110 AT3G28070.3 45.946 111 60 0 10 120 19 129 6.62e-11 58.5

Find 23 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATTGATGTTGAAAT+TGG 0.226936 5_1:-20729739 None:intergenic
TTCTGCAGATTACTTCTTCT+TGG 0.233740 5_1:+20729757 Msa0723110:CDS
ACTCATATGTTAATGACTTC+TGG 0.288657 5_1:+20729885 Msa0723110:CDS
ATAGCAAGTATGAAGGTAAA+AGG 0.300566 5_1:-20729960 None:intergenic
GTCAGAATTAACAAGCAAAT+TGG 0.319317 5_1:+20729494 Msa0723110:five_prime_UTR
TACTTCTTCTTGGTTGCCTC+AGG 0.379746 5_1:+20729767 Msa0723110:CDS
TCATACTTGCTATAATTTCA+AGG 0.384041 5_1:+20729970 Msa0723110:CDS
AAATATCAAAATTGTTAGGT+TGG 0.433167 5_1:-20729409 None:intergenic
ATGAAAGCAGCCACAAAAGA+TGG 0.452927 5_1:+20729619 Msa0723110:CDS
AAAATTGATGTTGAAATTGG+AGG 0.500522 5_1:-20729736 None:intergenic
CTATGGTGGAAGAGAGTGGA+TGG 0.506366 5_1:-20729700 None:intergenic
TGAAATTATAGCAAGTATGA+AGG 0.526932 5_1:-20729967 None:intergenic
AACAATAGCTGAAGCCAACA+TGG 0.534674 5_1:-20729925 None:intergenic
GAAGAAAACATAGAGCCAAA+AGG 0.540600 5_1:-20729675 None:intergenic
ATTGATGTTGAAATTGGAGG+AGG 0.546987 5_1:-20729733 None:intergenic
TAAATTCAGTTCTCCCATGT+TGG 0.547322 5_1:+20729911 Msa0723110:CDS
TGCAGCTCAAATTGTTGAAG+TGG 0.587755 5_1:+20729582 Msa0723110:CDS
GCAAGTATGAAGGTAAAAGG+AGG 0.605835 5_1:-20729957 None:intergenic
CAAGTATGAAGGTAAAAGGA+GGG 0.622141 5_1:-20729956 None:intergenic
GCAGCTCAAATTGTTGAAGT+GGG 0.628887 5_1:+20729583 Msa0723110:CDS
ACAATAGCTGAAGCCAACAT+GGG 0.645656 5_1:-20729924 None:intergenic
TCGGACTAAAGTTAAAGTTG+AGG 0.649103 5_1:+20729439 Msa0723110:five_prime_UTR
GCTCAAATTGTTGAAGTGGG+TGG 0.711164 5_1:+20729586 Msa0723110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATATCAAAATTGTT+AGG - chr5_1:20729416-20729435 None:intergenic 10.0%
!!! ATTTTGATATTTTTATTAGT+CGG + chr5_1:20729420-20729439 Msa0723110:five_prime_UTR 10.0%
!! ATTATTATAAAGATTGAATT+TGG + chr5_1:20729823-20729842 Msa0723110:intron 10.0%
!! AAATATCAAAATTGTTAGGT+TGG - chr5_1:20729412-20729431 None:intergenic 20.0%
!!! GGTATGTTGTTTTTATTTTA+TGG + chr5_1:20729991-20730010 Msa0723110:CDS 20.0%
!!! CGTCTTACTTTTATAATATC+TGG - chr5_1:20729475-20729494 None:intergenic 25.0%
!! CATTGTTTACAAAAACCTTT+TGG + chr5_1:20729660-20729679 Msa0723110:CDS 25.0%
! AAAATTGATGTTGAAATTGG+AGG - chr5_1:20729739-20729758 None:intergenic 25.0%
! CAGAAAATTGATGTTGAAAT+TGG - chr5_1:20729742-20729761 None:intergenic 25.0%
! TGAAATTATAGCAAGTATGA+AGG - chr5_1:20729970-20729989 None:intergenic 25.0%
!!! TCATACTTGCTATAATTTCA+AGG + chr5_1:20729970-20729989 Msa0723110:CDS 25.0%
GTCAGAATTAACAAGCAAAT+TGG + chr5_1:20729494-20729513 Msa0723110:five_prime_UTR 30.0%
! AATACTCATTCCATCTTTTG+TGG - chr5_1:20729632-20729651 None:intergenic 30.0%
TTTGGCAAAATAGAGTGATA+TGG + chr5_1:20729841-20729860 Msa0723110:intron 30.0%
ACTCATATGTTAATGACTTC+TGG + chr5_1:20729885-20729904 Msa0723110:CDS 30.0%
ATAGCAAGTATGAAGGTAAA+AGG - chr5_1:20729963-20729982 None:intergenic 30.0%
TCGGACTAAAGTTAAAGTTG+AGG + chr5_1:20729439-20729458 Msa0723110:five_prime_UTR 35.0%
CAGACAGAGACAAGAAAAAA+TGG + chr5_1:20729525-20729544 Msa0723110:exon 35.0%
GAAGAAAACATAGAGCCAAA+AGG - chr5_1:20729678-20729697 None:intergenic 35.0%
ATTGATGTTGAAATTGGAGG+AGG - chr5_1:20729736-20729755 None:intergenic 35.0%
TTCTGCAGATTACTTCTTCT+TGG + chr5_1:20729757-20729776 Msa0723110:CDS 35.0%
TACTATGTTGTTCATACCTG+AGG - chr5_1:20729786-20729805 None:intergenic 35.0%
TAAATTCAGTTCTCCCATGT+TGG + chr5_1:20729911-20729930 Msa0723110:CDS 35.0%
CAAGTATGAAGGTAAAAGGA+GGG - chr5_1:20729959-20729978 None:intergenic 35.0%
AGACAAGAAAAAATGGCGAG+TGG + chr5_1:20729532-20729551 Msa0723110:exon 40.0%
TGCAGCTCAAATTGTTGAAG+TGG + chr5_1:20729582-20729601 Msa0723110:CDS 40.0%
GCAGCTCAAATTGTTGAAGT+GGG + chr5_1:20729583-20729602 Msa0723110:CDS 40.0%
ATGAAAGCAGCCACAAAAGA+TGG + chr5_1:20729619-20729638 Msa0723110:CDS 40.0%
!! TTTTCTATGGTGGAAGAGAG+TGG - chr5_1:20729707-20729726 None:intergenic 40.0%
ACAATAGCTGAAGCCAACAT+GGG - chr5_1:20729927-20729946 None:intergenic 40.0%
AACAATAGCTGAAGCCAACA+TGG - chr5_1:20729928-20729947 None:intergenic 40.0%
GCAAGTATGAAGGTAAAAGG+AGG - chr5_1:20729960-20729979 None:intergenic 40.0%
CAAGAAAAAATGGCGAGTGG+AGG + chr5_1:20729535-20729554 Msa0723110:exon 45.0%
GCTCAAATTGTTGAAGTGGG+TGG + chr5_1:20729586-20729605 Msa0723110:CDS 45.0%
! GAGGAGCTCTTTTTCTATGG+TGG - chr5_1:20729717-20729736 None:intergenic 45.0%
! GAGGAGGAGCTCTTTTTCTA+TGG - chr5_1:20729720-20729739 None:intergenic 45.0%
TACTTCTTCTTGGTTGCCTC+AGG + chr5_1:20729767-20729786 Msa0723110:CDS 45.0%
! CTATGGTGGAAGAGAGTGGA+TGG - chr5_1:20729703-20729722 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20729408 20730025 20729408 ID=Msa0723110;Name=Msa0723110
chr5_1 mRNA 20729408 20730025 20729408 ID=Msa0723110-mRNA-1;Parent=Msa0723110;Name=Msa0723110-mRNA-1;_AED=0.40;_eAED=0.40;_QI=136|1|1|1|1|1|2|0|131
chr5_1 exon 20729408 20729788 20729408 ID=Msa0723110-mRNA-1:exon:7904;Parent=Msa0723110-mRNA-1
chr5_1 exon 20729878 20730025 20729878 ID=Msa0723110-mRNA-1:exon:7905;Parent=Msa0723110-mRNA-1
chr5_1 five_prime_UTR 20729408 20729543 20729408 ID=Msa0723110-mRNA-1:five_prime_utr;Parent=Msa0723110-mRNA-1
chr5_1 CDS 20729544 20729788 20729544 ID=Msa0723110-mRNA-1:cds;Parent=Msa0723110-mRNA-1
chr5_1 CDS 20729878 20730025 20729878 ID=Msa0723110-mRNA-1:cds;Parent=Msa0723110-mRNA-1
Gene Sequence

>Msa0723110

ATGGCGAGTGGAGGAGCAAGTGTAACAACAACTATGATTGCAGCTCAAATTGTTGAAGTGGGTGGTGAAACTCTAATGAAAGCAGCCACAAAAGATGGAATGAGTATTTTTATTTTCATTGTTTACAAAAACCTTTTGGCTCTATGTTTTCTTCTACCATCCACTCTCTTCCACCATAGAAAAAGAGCTCCTCCTCCAATTTCAACATCAATTTTCTGCAGATTACTTCTTCTTGGTTGCCTCAGAACTGCAACTCATATGTTAATGACTTCTGGAATTAAATTCAGTTCTCCCATGTTGGCTTCAGCTATTGTTAATCTTGTCCCTCCTTTTACCTTCATACTTGCTATAATTTCAAGGTATGTTGTTTTTATTTTATGGATTAGAATGCAA

Protein sequence

>Msa0723110

MASGGASVTTTMIAAQIVEVGGETLMKAATKDGMSIFIFIVYKNLLALCFLLPSTLFHHRKRAPPPISTSIFCRLLLLGCLRTATHMLMTSGIKFSSPMLASAIVNLVPPFTFILAIISRYVVFILWIRMQ