AlfalfaGEDB | MS.gene009874

M. sativa cultivar XinJiangDaYe / MS.gene009874

KEGG: K02942
GO: -
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009874.t1	XP_013464220.1	93.8	65	4	0	1	65	1	65	3.30E-25	124.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009874.t1	P52855	63.5	63	23	0	1	63	1	63	6.2e-17	88.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009874.t1	A0A072VJD3	93.8	65	4	0	1	65	1	65	2.3e-25	124.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene009874	MS.gene002442	PPI
MS.gene009874	MS.gene01091	PPI
MS.gene009874	MS.gene78764	PPI
MS.gene042719	MS.gene009874	PPI
MS.gene009874	MS.gene97876	PPI
MS.gene009874	MS.gene08521	PPI
MS.gene009874	MS.gene045633	PPI
MS.gene009874	MS.gene057237	PPI
MS.gene009874	MS.gene060483	PPI
MS.gene009874	MS.gene067331	PPI
MS.gene43071	MS.gene009874	PPI
MS.gene009874	MS.gene76615	PPI
MS.gene009874	MS.gene91826	PPI
MS.gene009874	MS.gene053952	PPI
MS.gene009874	MS.gene06833	PPI
MS.gene009874	MS.gene01425	PPI
MS.gene009874	MS.gene82009	PPI
MS.gene009874	MS.gene44900	PPI
MS.gene009874	MS.gene013506	PPI
MS.gene009874	MS.gene032942	PPI
MS.gene44900	MS.gene009874	PPI
MS.gene009874	MS.gene35379	PPI
MS.gene009874	MS.gene01952	PPI
MS.gene009874	MS.gene069339	PPI
MS.gene009874	MS.gene06617	PPI
MS.gene073078	MS.gene009874	PPI
MS.gene009874	MS.gene88792	PPI
MS.gene009874	MS.gene047578	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009874.t1	MTR_3g092600	93.443	61	4	0	1	61	1	61	2.45e-35	118
MS.gene009874.t1	MTR_2g066750	93.443	61	4	0	1	61	1	61	2.45e-35	118
MS.gene009874.t1	MTR_8g024350	63.889	72	25	1	1	72	1	71	1.45e-25	94.0
MS.gene009874.t1	MTR_7g118060	61.017	59	23	0	1	59	1	59	3.19e-19	77.8
MS.gene009874.t1	MTR_7g005740	61.290	62	5	1	1	62	1	43	6.52e-16	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009874.t1	AT5G24510	64.789	71	24	1	1	71	1	70	2.07e-26	95.9
MS.gene009874.t1	AT5G47700	63.158	57	21	0	5	61	6	62	1.60e-20	81.3
MS.gene009874.t1	AT5G47700	63.158	57	21	0	5	61	6	62	1.60e-20	81.3
MS.gene009874.t1	AT1G01100	61.404	57	22	0	5	61	6	62	2.88e-20	80.1
MS.gene009874.t1	AT1G01100	61.404	57	22	0	5	61	6	62	4.86e-20	79.7
MS.gene009874.t1	AT1G01100	61.404	57	22	0	5	61	6	62	4.86e-20	79.7
MS.gene009874.t1	AT1G01100	61.404	57	22	0	5	61	6	62	4.86e-20	79.7
MS.gene009874.t1	AT4G00810	59.649	57	23	0	5	61	6	62	2.44e-19	78.2
MS.gene009874.t1	AT4G00810	59.649	57	23	0	5	61	6	62	2.44e-19	78.2

Find 41 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGTATTTGCACCTGCTTC+TGG	0.225261	5.3:-56003912	MS.gene009874:CDS
AGATAGTTGATTGCAAGTTT+TGG	0.308451	5.3:+56003734	None:intergenic
GTCATCGTCACTTTCTTCCT+TGG	0.329724	5.3:+56003840	None:intergenic
ACGTCACCGTTGAATCTTAC+TGG	0.348075	5.3:-56004019	MS.gene009874:CDS
GATAGCAGACTTACGGTGAT+AGG	0.389185	5.3:+56004156	None:intergenic
CCAGACTAGGACTCCTTTCA+AGG	0.415086	5.3:-56003769	MS.gene009874:CDS
CCTTGAAAGGAGTCCTAGTC+TGG	0.418177	5.3:+56003769	None:intergenic
CAAAATGAGTTCCAACGAAT+CGG	0.420231	5.3:-56004220	None:intergenic
TTTGCACCTGCTTCTGGAGG+TGG	0.421586	5.3:-56003906	MS.gene009874:CDS
TCTGAGCAAGCTTAGCGAAT+AGG	0.441352	5.3:+56003991	None:intergenic
TCCAGAAGCAGGTGCAAATA+CGG	0.456176	5.3:+56003911	None:intergenic
AACTGCAGCCGTTGAAAAGA+AGG	0.478736	5.3:-56003869	MS.gene009874:CDS
GAAGATCGGTACTTTGTTGA+AGG	0.484661	5.3:-56004048	MS.gene009874:CDS
AAAATGAGTTCCAACGAATC+GGG	0.488892	5.3:-56004219	MS.gene009874:CDS
ACCGTTGAATCTTACTGGCC+AGG	0.494210	5.3:-56004014	MS.gene009874:CDS
GCGGCTCCACCTCCAGAAGC+AGG	0.495490	5.3:+56003900	None:intergenic
CGGCTGCAGTTCGGTTGTTG+CGG	0.504668	5.3:+56003881	None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	5.3:-56003945	MS.gene009874:CDS
TAGTTTGTTGTAGCCAGACT+AGG	0.521961	5.3:-56003782	MS.gene009874:CDS
GGAAGAAAGTGACGATGACA+TGG	0.523361	5.3:-56003836	MS.gene009874:CDS
AGATTCAACGGTGACGTTAG+CGG	0.526016	5.3:+56004025	None:intergenic
GCAAGCTTAGCGAATAGGCC+TGG	0.531593	5.3:+56003996	None:intergenic
TTTGTTTAGGCGAAGAAGAT+CGG	0.532771	5.3:-56004062	MS.gene009874:intron
TCCATCGTCGTGGAGGATCA+AGG	0.532980	5.3:+56004182	None:intergenic
ATAGCAGACTTACGGTGATA+GGG	0.561399	5.3:+56004157	None:intergenic
ACCTTGATCCTCCACGACGA+TGG	0.573503	5.3:-56004183	MS.gene009874:CDS
TGAAAAGAAGGAGGAAGCCA+AGG	0.574510	5.3:-56003857	MS.gene009874:CDS
GTAAATGCAGCCCGATTCGT+TGG	0.581909	5.3:+56004209	None:intergenic
CAGACTAGGACTCCTTTCAA+GGG	0.583004	5.3:-56003768	MS.gene009874:CDS
GGTGGCACCACCAACAGCGC+CGG	0.584690	5.3:+56003938	None:intergenic
GCCTGGCCAGTAAGATTCAA+CGG	0.587082	5.3:+56004013	None:intergenic
AGCAGGTGCAAATACGGCAA+CGG	0.623922	5.3:+56003917	None:intergenic
GTATTTGCACCTGCTTCTGG+AGG	0.630481	5.3:-56003909	MS.gene009874:CDS
AGACTAGGACTCCTTTCAAG+GGG	0.655500	5.3:-56003767	MS.gene009874:CDS
AGGTGCAAATACGGCAACGG+TGG	0.659394	5.3:+56003920	None:intergenic
TGATAGGGATTCCATCGTCG+TGG	0.665821	5.3:+56004172	None:intergenic
TAGGGATTCCATCGTCGTGG+AGG	0.668252	5.3:+56004175	None:intergenic
TGCAGCCGTTGAAAAGAAGG+AGG	0.676132	5.3:-56003866	MS.gene009874:CDS
GAAGAAAGTGACGATGACAT+GGG	0.678372	5.3:-56003835	MS.gene009874:CDS
GACTAGGACTCCTTTCAAGG+GGG	0.714058	5.3:-56003766	MS.gene009874:CDS
ATCGTCGTGGAGGATCAAGG+TGG	0.743990	5.3:+56004185	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACAGAAATAGAATTGAATA+GGG	+	chr5.3:56003798-56003817	None:intergenic	20.0%
!!	TTACAGAAATAGAATTGAAT+AGG	+	chr5.3:56003799-56003818	None:intergenic	20.0%
!!!	ATTTTTCTTTGTTACTGTTT+GGG	-	chr5.3:56003842-56003861	MS.gene009874:CDS	20.0%
!!	CATTTTTCTTTGTTACTGTT+TGG	-	chr5.3:56003841-56003860	MS.gene009874:CDS	25.0%
!	AGATAGTTGATTGCAAGTTT+TGG	+	chr5.3:56004198-56004217	None:intergenic	30.0%
!!	TACTGTTTGGGTTTTTGTTT+AGG	-	chr5.3:56003854-56003873	MS.gene009874:CDS	30.0%
	AATGAGATCTATGAGCGATT+TGG	+	chr5.3:56003825-56003844	None:intergenic	35.0%
	TTTGTTTAGGCGAAGAAGAT+CGG	-	chr5.3:56003867-56003886	MS.gene009874:CDS	35.0%
	ATAGCAGACTTACGGTGATA+GGG	+	chr5.3:56003775-56003794	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr5.3:56004094-56004113	MS.gene009874:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr5.3:56003881-56003900	MS.gene009874:CDS	40.0%
	GAATAGGGATAGCAGACTTA+CGG	+	chr5.3:56003783-56003802	None:intergenic	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr5.3:56003972-56003991	MS.gene009874:CDS	40.0%
!!	TAGTTTGTTGTAGCCAGACT+AGG	-	chr5.3:56004147-56004166	MS.gene009874:intron	40.0%
	AACTGCAGCCGTTGAAAAGA+AGG	-	chr5.3:56004060-56004079	MS.gene009874:intron	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr5.3:56003910-56003929	MS.gene009874:CDS	45.0%
	AGACTAGGACTCCTTTCAAG+GGG	-	chr5.3:56004162-56004181	MS.gene009874:intron	45.0%
	AGATTCAACGGTGACGTTAG+CGG	+	chr5.3:56003907-56003926	None:intergenic	45.0%
	CAGACTAGGACTCCTTTCAA+GGG	-	chr5.3:56004161-56004180	MS.gene009874:intron	45.0%
	GATAGCAGACTTACGGTGAT+AGG	+	chr5.3:56003776-56003795	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr5.3:56004093-56004112	MS.gene009874:intron	45.0%
	TCCAGAAGCAGGTGCAAATA+CGG	+	chr5.3:56004021-56004040	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr5.3:56003941-56003960	None:intergenic	45.0%
	TGAAAAGAAGGAGGAAGCCA+AGG	-	chr5.3:56004072-56004091	MS.gene009874:intron	45.0%
!	GGCTTCCTCCTTCTTTTCAA+CGG	+	chr5.3:56004071-56004090	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr5.3:56004092-56004111	None:intergenic	45.0%
!	TCTTTTCAACGGCTGCAGTT+CGG	+	chr5.3:56004060-56004079	None:intergenic	45.0%
	ACCGTTGAATCTTACTGGCC+AGG	-	chr5.3:56003915-56003934	MS.gene009874:CDS	50.0%
	AGCAGGTGCAAATACGGCAA+CGG	+	chr5.3:56004015-56004034	None:intergenic	50.0%
	CCAGACTAGGACTCCTTTCA+AGG	-	chr5.3:56004160-56004179	MS.gene009874:intron	50.0%
	CCTTGAAAGGAGTCCTAGTC+TGG	+	chr5.3:56004163-56004182	None:intergenic	50.0%
	GACTAGGACTCCTTTCAAGG+GGG	-	chr5.3:56004163-56004182	MS.gene009874:intron	50.0%
	GCCTGGCCAGTAAGATTCAA+CGG	+	chr5.3:56003919-56003938	None:intergenic	50.0%
	GTATTTGCACCTGCTTCTGG+AGG	-	chr5.3:56004020-56004039	MS.gene009874:CDS	50.0%
	TGATAGGGATTCCATCGTCG+TGG	+	chr5.3:56003760-56003779	None:intergenic	50.0%
	TGCAGCCGTTGAAAAGAAGG+AGG	-	chr5.3:56004063-56004082	MS.gene009874:intron	50.0%
!	GTAAATGCAGCCCGATTCGT+TGG	+	chr5.3:56003723-56003742	None:intergenic	50.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr5.3:56003746-56003765	MS.gene009874:CDS	55.0%
	AGGTGCAAATACGGCAACGG+TGG	+	chr5.3:56004012-56004031	None:intergenic	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr5.3:56003747-56003766	None:intergenic	55.0%
	GATTCAGCCACCCCCTTGAA+AGG	+	chr5.3:56004176-56004195	None:intergenic	55.0%
	GCAAGCTTAGCGAATAGGCC+TGG	+	chr5.3:56003936-56003955	None:intergenic	55.0%
	GCCGTATTTGCACCTGCTTC+TGG	-	chr5.3:56004017-56004036	MS.gene009874:CDS	55.0%
	TAGGACTCCTTTCAAGGGGG+TGG	-	chr5.3:56004166-56004185	MS.gene009874:intron	55.0%
	TAGGGATTCCATCGTCGTGG+AGG	+	chr5.3:56003757-56003776	None:intergenic	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr5.3:56003750-56003769	None:intergenic	55.0%
!	TTTGCACCTGCTTCTGGAGG+TGG	-	chr5.3:56004023-56004042	MS.gene009874:CDS	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr5.3:56003981-56004000	MS.gene009874:CDS	55.0%
	CGGCTGCAGTTCGGTTGTTG+CGG	+	chr5.3:56004051-56004070	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr5.3:56003984-56004003	MS.gene009874:CDS	60.0%
	GCGGCTCCACCTCCAGAAGC+AGG	+	chr5.3:56004032-56004051	None:intergenic	70.0%
	GGTGGCACCACCAACAGCGC+CGG	+	chr5.3:56003994-56004013	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	56003713	56004238	56003713	ID=MS.gene009874
chr5.3	mRNA	56003713	56004238	56003713	ID=MS.gene009874.t1;Parent=MS.gene009874
chr5.3	exon	56004170	56004238	56004170	ID=MS.gene009874.t1.exon1;Parent=MS.gene009874.t1
chr5.3	CDS	56004170	56004238	56004170	ID=cds.MS.gene009874.t1;Parent=MS.gene009874.t1
chr5.3	exon	56003713	56004075	56003713	ID=MS.gene009874.t1.exon2;Parent=MS.gene009874.t1
chr5.3	CDS	56003713	56004075	56003713	ID=cds.MS.gene009874.t1;Parent=MS.gene009874.t1

Gene Sequence

>MS.gene009874

ATGAGTTCCAACGAATCGGGCTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCTATCACCGTAAGTCTGCTATCCCTATTCAATTCTATTTCTGTAATCTCCAAATCGCTCATAGATCTCATTTTTCTTTGTTACTGTTTGGGTTTTTGTTTAGGCGAAGAAGATCGGTACTTTGTTGAAGGCCGCTAACGTCACCGTTGAATCTTACTGGCCAGGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTCGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCACCGTTGCCGTATTTGCACCTGCTTCTGGAGGTGGAGCCGCAACAACCGAACTGCAGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAAGTTTCTCTGTATAGTTTGTTGTAGCCAGACTAGGACTCCTTTCAAGGGGGTGGCTGAATCCAAAACTTGCAATCAACTATCTCTTTATAAATTGTTTATCTAA

Protein sequence

>MS.gene009874.t1

MSSNESGCIYATLILHDDGIPITAKKIGTLLKAANVTVESYWPGLFAKLAQSKNVDDLVLNSGAVGGATVAVFAPASGGGAATTELQPLKRRRKPRKKVTMTWALVCLIKFLCIVCCSQTRTPFKGVAESKTCNQLSLYKLFI