AlfalfaGEDB | MS.gene44900

M. sativa cultivar XinJiangDaYe / MS.gene44900

KEGG: K02942
GO: -
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44900.t1	XP_013464220.1	93.8	65	4	0	1	65	1	65	4.20E-25	124.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44900.t1	P52855	63.5	63	23	0	1	63	1	63	8.1e-17	88.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44900.t1	A0A072VJD3	93.8	65	4	0	1	65	1	65	3.1e-25	124.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene44900	MS.gene045633	PPI
MS.gene042719	MS.gene44900	PPI
MS.gene44900	MS.gene057237	PPI
MS.gene44900	MS.gene060483	PPI
MS.gene44900	MS.gene01091	PPI
MS.gene44900	MS.gene91826	PPI
MS.gene44900	MS.gene78764	PPI
MS.gene44900	MS.gene97876	PPI
MS.gene44900	MS.gene053952	PPI
MS.gene44900	MS.gene08521	PPI
MS.gene44900	MS.gene01425	PPI
MS.gene44900	MS.gene82009	PPI
MS.gene073078	MS.gene44900	PPI
MS.gene44900	MS.gene067331	PPI
MS.gene44900	MS.gene76615	PPI
MS.gene44900	MS.gene01952	PPI
MS.gene009874	MS.gene44900	PPI
MS.gene44900	MS.gene06833	PPI
MS.gene44900	MS.gene013506	PPI
MS.gene44900	MS.gene009874	PPI
MS.gene44900	MS.gene032942	PPI
MS.gene44900	MS.gene35379	PPI
MS.gene44900	MS.gene069339	PPI
MS.gene44900	MS.gene06617	PPI
MS.gene44900	MS.gene88792	PPI
MS.gene44900	MS.gene047578	PPI
MS.gene44900	MS.gene002442	PPI
MS.gene43071	MS.gene44900	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44900.t1	MTR_3g092600	93.443	61	4	0	1	61	1	61	2.79e-35	118
MS.gene44900.t1	MTR_2g066750	93.443	61	4	0	1	61	1	61	2.79e-35	118
MS.gene44900.t1	MTR_8g024350	62.500	72	26	1	1	72	1	71	8.06e-25	92.0
MS.gene44900.t1	MTR_7g118060	61.017	59	23	0	1	59	1	59	3.48e-19	77.8
MS.gene44900.t1	MTR_7g005740	61.290	62	5	1	1	62	1	43	6.80e-16	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44900.t1	AT5G24510	64.789	71	24	1	1	71	1	70	1.57e-26	96.3
MS.gene44900.t1	AT5G47700	63.158	57	21	0	5	61	6	62	2.03e-20	80.9
MS.gene44900.t1	AT5G47700	63.158	57	21	0	5	61	6	62	2.03e-20	80.9
MS.gene44900.t1	AT1G01100	61.404	57	22	0	5	61	6	62	3.58e-20	79.7
MS.gene44900.t1	AT1G01100	61.404	57	22	0	5	61	6	62	5.78e-20	79.7
MS.gene44900.t1	AT1G01100	61.404	57	22	0	5	61	6	62	5.78e-20	79.7
MS.gene44900.t1	AT1G01100	61.404	57	22	0	5	61	6	62	5.78e-20	79.7
MS.gene44900.t1	AT4G00810	59.649	57	23	0	5	61	6	62	2.81e-19	77.8
MS.gene44900.t1	AT4G00810	59.649	57	23	0	5	61	6	62	2.81e-19	77.8

Find 38 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGTATTTGCACTTGCTTC+TGG	0.230104	5.2:-59482159	MS.gene44900:CDS
AGATAGTAGATTGCAAGTTT+TGG	0.304388	5.2:+59481981	None:intergenic
GTCATCGTCACTTTCTTCCT+TGG	0.329724	5.2:+59482087	None:intergenic
ACGTCACTGTTGAATCTTAC+TGG	0.343339	5.2:-59482266	MS.gene44900:CDS
GATAGCAGACTTACGGTGAT+AGG	0.389185	5.2:+59482403	None:intergenic
CAAAATGAGTTCCAACGAAT+CGG	0.399254	5.2:-59482467	None:intergenic
CCTTGAAAGGAGTCCTAGTC+TGG	0.410741	5.2:+59482016	None:intergenic
TTTGCACTTGCTTCTGGAGG+TGG	0.412215	5.2:-59482153	MS.gene44900:CDS
CCAGACTAGGACTCCTTTCA+AGG	0.415086	5.2:-59482016	MS.gene44900:CDS
TCCAGAAGCAAGTGCAAATA+CGG	0.439030	5.2:+59482158	None:intergenic
TCTGAGCAAGCTTAGCGAAT+AGG	0.441352	5.2:+59482238	None:intergenic
AACTGCAGCCGTTGAAAAGA+AGG	0.478736	5.2:-59482116	MS.gene44900:CDS
GAAGATCGGTACTTTGTTGA+AGG	0.484661	5.2:-59482295	MS.gene44900:CDS
AAAATGAGTTCCAACGAATC+GGG	0.497361	5.2:-59482466	MS.gene44900:CDS
CGGCTGCAGTTCGGTTGTTG+CGG	0.504668	5.2:+59482128	None:intergenic
ACTGTTGAATCTTACTGGCC+AGG	0.517027	5.2:-59482261	MS.gene44900:CDS
TTGAACTCCGGCGCTGTTGG+TGG	0.521380	5.2:-59482192	MS.gene44900:CDS
TAGTTTGTTGTAGCCAGACT+AGG	0.521961	5.2:-59482029	MS.gene44900:CDS
GGAAGAAAGTGACGATGACA+TGG	0.523361	5.2:-59482083	MS.gene44900:CDS
GCAAGCTTAGCGAATAGGCC+TGG	0.531593	5.2:+59482243	None:intergenic
TTTGTTTAGGCGAAGAAGAT+CGG	0.532771	5.2:-59482309	MS.gene44900:intron
TCCATCGTCGTGGAGGATCA+AGG	0.532980	5.2:+59482429	None:intergenic
GGCGACACCACCAACAGCGC+CGG	0.535107	5.2:+59482185	None:intergenic
ATAGCAGACTTACGGTGATA+GGG	0.561399	5.2:+59482404	None:intergenic
GTAAATGCAACCCGATTCGT+TGG	0.564773	5.2:+59482456	None:intergenic
ACCTTGATCCTCCACGACGA+TGG	0.573503	5.2:-59482430	MS.gene44900:CDS
TGAAAAGAAGGAGGAAGCCA+AGG	0.574510	5.2:-59482104	MS.gene44900:CDS
CAGACTAGGACTCCTTTCAA+GGG	0.582594	5.2:-59482015	MS.gene44900:CDS
AGATTCAACAGTGACGTTAG+CGG	0.589927	5.2:+59482272	None:intergenic
GTATTTGCACTTGCTTCTGG+AGG	0.593602	5.2:-59482156	MS.gene44900:CDS
AGCAAGTGCAAATACGGCAA+CGG	0.622430	5.2:+59482164	None:intergenic
AGACTAGGACTCCTTTCAAG+GGG	0.655090	5.2:-59482014	MS.gene44900:CDS
TGATAGGGATTCCATCGTCG+TGG	0.665821	5.2:+59482419	None:intergenic
TAGGGATTCCATCGTCGTGG+AGG	0.668252	5.2:+59482422	None:intergenic
TGCAGCCGTTGAAAAGAAGG+AGG	0.676132	5.2:-59482113	MS.gene44900:CDS
GAAGAAAGTGACGATGACAT+GGG	0.678372	5.2:-59482082	MS.gene44900:CDS
GACTAGGACTCCTTTCAAGG+GGG	0.717397	5.2:-59482013	MS.gene44900:CDS
ATCGTCGTGGAGGATCAAGG+TGG	0.743990	5.2:+59482432	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACAGAAATAGAATTGAATA+GGG	+	chr5.2:59482045-59482064	None:intergenic	20.0%
!!	TTACAGAAATAGAATTGAAT+AGG	+	chr5.2:59482046-59482065	None:intergenic	20.0%
!!!	ATTTTTCTTTGTTACTGTTT+GGG	-	chr5.2:59482089-59482108	MS.gene44900:CDS	20.0%
!!	CATTTTTCTTTGTTACTGTT+TGG	-	chr5.2:59482088-59482107	MS.gene44900:CDS	25.0%
!	AGATAGTAGATTGCAAGTTT+TGG	+	chr5.2:59482445-59482464	None:intergenic	30.0%
!!	TACTGTTTGGGTTTTTGTTT+AGG	-	chr5.2:59482101-59482120	MS.gene44900:CDS	30.0%
	AATGAGATCTATGAGCGATT+TGG	+	chr5.2:59482072-59482091	None:intergenic	35.0%
	TTTGTTTAGGCGAAGAAGAT+CGG	-	chr5.2:59482114-59482133	MS.gene44900:CDS	35.0%
	ACGTCACTGTTGAATCTTAC+TGG	-	chr5.2:59482157-59482176	MS.gene44900:CDS	40.0%
	AGATTCAACAGTGACGTTAG+CGG	+	chr5.2:59482154-59482173	None:intergenic	40.0%
	ATAGCAGACTTACGGTGATA+GGG	+	chr5.2:59482022-59482041	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr5.2:59482341-59482360	MS.gene44900:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr5.2:59482128-59482147	MS.gene44900:CDS	40.0%
	GAATAGGGATAGCAGACTTA+CGG	+	chr5.2:59482030-59482049	None:intergenic	40.0%
	TCCAGAAGCAAGTGCAAATA+CGG	+	chr5.2:59482268-59482287	None:intergenic	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr5.2:59482219-59482238	MS.gene44900:CDS	40.0%
!!	TAGTTTGTTGTAGCCAGACT+AGG	-	chr5.2:59482394-59482413	MS.gene44900:intron	40.0%
	AACTGCAGCCGTTGAAAAGA+AGG	-	chr5.2:59482307-59482326	MS.gene44900:intron	45.0%
	ACTGTTGAATCTTACTGGCC+AGG	-	chr5.2:59482162-59482181	MS.gene44900:CDS	45.0%
	AGACTAGGACTCCTTTCAAG+GGG	-	chr5.2:59482409-59482428	MS.gene44900:intron	45.0%
	AGCAAGTGCAAATACGGCAA+CGG	+	chr5.2:59482262-59482281	None:intergenic	45.0%
	CAGACTAGGACTCCTTTCAA+GGG	-	chr5.2:59482408-59482427	MS.gene44900:intron	45.0%
	GATAGCAGACTTACGGTGAT+AGG	+	chr5.2:59482023-59482042	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr5.2:59482340-59482359	MS.gene44900:intron	45.0%
	GTATTTGCACTTGCTTCTGG+AGG	-	chr5.2:59482267-59482286	MS.gene44900:CDS	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr5.2:59482188-59482207	None:intergenic	45.0%
	TGAAAAGAAGGAGGAAGCCA+AGG	-	chr5.2:59482319-59482338	MS.gene44900:intron	45.0%
!	GGCTTCCTCCTTCTTTTCAA+CGG	+	chr5.2:59482318-59482337	None:intergenic	45.0%
!	GTAAATGCAACCCGATTCGT+TGG	+	chr5.2:59481970-59481989	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr5.2:59482339-59482358	None:intergenic	45.0%
!	TCTTTTCAACGGCTGCAGTT+CGG	+	chr5.2:59482307-59482326	None:intergenic	45.0%
	CCAGACTAGGACTCCTTTCA+AGG	-	chr5.2:59482407-59482426	MS.gene44900:intron	50.0%
	CCTTGAAAGGAGTCCTAGTC+TGG	+	chr5.2:59482410-59482429	None:intergenic	50.0%
	GACTAGGACTCCTTTCAAGG+GGG	-	chr5.2:59482410-59482429	MS.gene44900:intron	50.0%
	GCCGTATTTGCACTTGCTTC+TGG	-	chr5.2:59482264-59482283	MS.gene44900:CDS	50.0%
	TGATAGGGATTCCATCGTCG+TGG	+	chr5.2:59482007-59482026	None:intergenic	50.0%
	TGCAGCCGTTGAAAAGAAGG+AGG	-	chr5.2:59482310-59482329	MS.gene44900:intron	50.0%
!	TTTGCACTTGCTTCTGGAGG+TGG	-	chr5.2:59482270-59482289	MS.gene44900:CDS	50.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr5.2:59481993-59482012	MS.gene44900:CDS	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr5.2:59481994-59482013	None:intergenic	55.0%
	GATTCAGCCTCCCCCTTGAA+AGG	+	chr5.2:59482423-59482442	None:intergenic	55.0%
	GCAAGCTTAGCGAATAGGCC+TGG	+	chr5.2:59482183-59482202	None:intergenic	55.0%
	TAGGACTCCTTTCAAGGGGG+AGG	-	chr5.2:59482413-59482432	MS.gene44900:intron	55.0%
	TAGGGATTCCATCGTCGTGG+AGG	+	chr5.2:59482004-59482023	None:intergenic	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr5.2:59481997-59482016	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr5.2:59482228-59482247	MS.gene44900:CDS	55.0%
	CGGCTGCAGTTCGGTTGTTG+CGG	+	chr5.2:59482298-59482317	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr5.2:59482231-59482250	MS.gene44900:CDS	60.0%
	GGCGACACCACCAACAGCGC+CGG	+	chr5.2:59482241-59482260	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	59481960	59482485	59481960	ID=MS.gene44900
chr5.2	mRNA	59481960	59482485	59481960	ID=MS.gene44900.t1;Parent=MS.gene44900
chr5.2	exon	59482417	59482485	59482417	ID=MS.gene44900.t1.exon1;Parent=MS.gene44900.t1
chr5.2	CDS	59482417	59482485	59482417	ID=cds.MS.gene44900.t1;Parent=MS.gene44900.t1
chr5.2	exon	59481960	59482322	59481960	ID=MS.gene44900.t1.exon2;Parent=MS.gene44900.t1
chr5.2	CDS	59481960	59482322	59481960	ID=cds.MS.gene44900.t1;Parent=MS.gene44900.t1

Gene Sequence

>MS.gene44900

ATGAGTTCCAACGAATCGGGTTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCTATCACCGTAAGTCTGCTATCCCTATTCAATTCTATTTCTGTAATCTCCAAATCGCTCATAGATCTCATTTTTCTTTGTTACTGTTTGGGTTTTTGTTTAGGCGAAGAAGATCGGTACTTTGTTGAAGGCCGCTAACGTCACTGTTGAATCTTACTGGCCAGGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGTCGCCGTTGCCGTATTTGCACTTGCTTCTGGAGGTGGAGCCGCAACAACCGAACTGCAGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAAGTTTCTATGTATAGTTTGTTGTAGCCAGACTAGGACTCCTTTCAAGGGGGAGGCTGAATCCAAAACTTGCAATCTACTATCTCTTTATAAATTGTTTATCTAA

Protein sequence

>MS.gene44900.t1

MSSNESGCIYATLILHDDGIPITAKKIGTLLKAANVTVESYWPGLFAKLAQSKNVDDLVLNSGAVGGVAVAVFALASGGGAATTELQPLKRRRKPRKKVTMTWALVCLIKFLCIVCCSQTRTPFKGEAESKTCNLLSLYKLFI