Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76615.t1 | XP_013464220.1 | 90.8 | 65 | 6 | 0 | 1 | 65 | 1 | 65 | 4.90E-24 | 120.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76615.t1 | P52855 | 61.9 | 63 | 24 | 0 | 1 | 63 | 1 | 63 | 3.2e-16 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76615.t1 | A0A072VJD3 | 90.8 | 65 | 6 | 0 | 1 | 65 | 1 | 65 | 3.5e-24 | 120.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76615 | MS.gene01952 | PPI |
| MS.gene76615 | MS.gene82009 | PPI |
| MS.gene82009 | MS.gene76615 | PPI |
| MS.gene073078 | MS.gene76615 | PPI |
| MS.gene76615 | MS.gene032942 | PPI |
| MS.gene069339 | MS.gene76615 | PPI |
| MS.gene76615 | MS.gene35379 | PPI |
| MS.gene76615 | MS.gene06617 | PPI |
| MS.gene009874 | MS.gene76615 | PPI |
| MS.gene76615 | MS.gene88792 | PPI |
| MS.gene76615 | MS.gene047578 | PPI |
| MS.gene44900 | MS.gene76615 | PPI |
| MS.gene76615 | MS.gene002442 | PPI |
| MS.gene76615 | MS.gene045633 | PPI |
| MS.gene76615 | MS.gene057237 | PPI |
| MS.gene76615 | MS.gene01091 | PPI |
| MS.gene76615 | MS.gene91826 | PPI |
| MS.gene76615 | MS.gene78764 | PPI |
| MS.gene76615 | MS.gene97876 | PPI |
| MS.gene76615 | MS.gene053952 | PPI |
| MS.gene76615 | MS.gene08521 | PPI |
| MS.gene43071 | MS.gene76615 | PPI |
| MS.gene76615 | MS.gene01425 | PPI |
| MS.gene08521 | MS.gene76615 | PPI |
| MS.gene042719 | MS.gene76615 | PPI |
| MS.gene06833 | MS.gene76615 | PPI |
| MS.gene013506 | MS.gene76615 | PPI |
| MS.gene76615 | MS.gene060483 | PPI |
| MS.gene76615 | MS.gene067331 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76615.t1 | MTR_3g092600 | 90.164 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 1.90e-33 | 114 |
| MS.gene76615.t1 | MTR_2g066750 | 90.164 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 1.90e-33 | 114 |
| MS.gene76615.t1 | MTR_8g024350 | 64.384 | 73 | 25 | 1 | 1 | 73 | 1 | 72 | 4.25e-26 | 95.5 |
| MS.gene76615.t1 | MTR_7g118060 | 61.017 | 59 | 23 | 0 | 1 | 59 | 1 | 59 | 1.53e-19 | 78.6 |
| MS.gene76615.t1 | MTR_7g005740 | 58.065 | 62 | 7 | 1 | 1 | 62 | 1 | 43 | 9.25e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76615.t1 | AT5G24510 | 51.064 | 94 | 45 | 1 | 1 | 94 | 1 | 93 | 1.62e-25 | 94.0 |
| MS.gene76615.t1 | AT5G47700 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 3.82e-20 | 80.5 |
| MS.gene76615.t1 | AT5G47700 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 3.82e-20 | 80.5 |
| MS.gene76615.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 7.09e-20 | 79.3 |
| MS.gene76615.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.21e-19 | 79.0 |
| MS.gene76615.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.21e-19 | 79.0 |
| MS.gene76615.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.21e-19 | 79.0 |
| MS.gene76615.t1 | AT4G00810 | 56.667 | 60 | 26 | 0 | 2 | 61 | 3 | 62 | 5.73e-19 | 77.4 |
| MS.gene76615.t1 | AT4G00810 | 56.667 | 60 | 26 | 0 | 2 | 61 | 3 | 62 | 5.73e-19 | 77.4 |
Find 38 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGTATCTGCACCTGCTTC+TGG | 0.256570 | 5.1:-31356592 | MS.gene76615:CDS |
| AGATAGTAGATTGCAAGTTT+TGG | 0.304388 | 5.1:+31356411 | None:intergenic |
| GTCATCGTCACTTTCTTCCT+TGG | 0.339396 | 5.1:+31356517 | None:intergenic |
| ACGTCACCGTTGAATCTTAC+TGG | 0.348075 | 5.1:-31356699 | MS.gene76615:CDS |
| CAAAATGAGTTTCGGCGAAT+CGG | 0.351766 | 5.1:-31356900 | None:intergenic |
| AAAATGAGTTTCGGCGAATC+GGG | 0.370184 | 5.1:-31356899 | MS.gene76615:CDS |
| ACAATTCACAAAATGAGTTT+CGG | 0.409091 | 5.1:-31356908 | None:intergenic |
| CCAGACTAGGACTCCTTTCA+AGG | 0.415086 | 5.1:-31356446 | MS.gene76615:CDS |
| CCTTGAAAGGAGTCCTAGTC+TGG | 0.418177 | 5.1:+31356446 | None:intergenic |
| TATGAGCAAGCTTAGCGAAT+AGG | 0.439056 | 5.1:+31356671 | None:intergenic |
| TCTGCACCTGCTTCTGGAGG+TGG | 0.452148 | 5.1:-31356586 | MS.gene76615:CDS |
| GATAGCAGACTTACGGGGAT+AGG | 0.472811 | 5.1:+31356836 | None:intergenic |
| GAAGATCGGTACTTTGTTGA+AGG | 0.484661 | 5.1:-31356728 | MS.gene76615:CDS |
| ACCGTTGAATCTTACTGGCC+AGG | 0.494210 | 5.1:-31356694 | MS.gene76615:CDS |
| GCGGCTCCACCTCCAGAAGC+AGG | 0.495490 | 5.1:+31356580 | None:intergenic |
| TTGAACTCCGGCGCTGTTGG+TGG | 0.510539 | 5.1:-31356625 | MS.gene76615:CDS |
| TCCAGAAGCAGGTGCAGATA+CGG | 0.511601 | 5.1:+31356591 | None:intergenic |
| ATAGCAGACTTACGGGGATA+GGG | 0.518236 | 5.1:+31356837 | None:intergenic |
| TAGTTTGTTGTAGCCAGACT+AGG | 0.521961 | 5.1:-31356459 | MS.gene76615:CDS |
| GGAAGAAAGTGACGATGACA+TGG | 0.523361 | 5.1:-31356513 | MS.gene76615:CDS |
| AGATTCAACGGTGACGTTAG+CGG | 0.526016 | 5.1:+31356705 | None:intergenic |
| TTTGTTTAGGCGAAGAAGAT+CGG | 0.532771 | 5.1:-31356742 | MS.gene76615:intron |
| TCCATCGTCGTGGAGGATCA+AGG | 0.532980 | 5.1:+31356862 | None:intergenic |
| GCAAGCTTAGCGAATAGGCC+TGG | 0.533765 | 5.1:+31356676 | None:intergenic |
| TGCGGCACCACCAACAGCGC+CGG | 0.548111 | 5.1:+31356618 | None:intergenic |
| ACCTTGATCCTCCACGACGA+TGG | 0.573503 | 5.1:-31356863 | MS.gene76615:CDS |
| CAGACTAGGACTCCTTTCAA+GGG | 0.583004 | 5.1:-31356445 | MS.gene76615:CDS |
| GCCTGGCCAGTAAGATTCAA+CGG | 0.587082 | 5.1:+31356693 | None:intergenic |
| CGGCAGTTCGGCTACTGTTG+CGG | 0.596856 | 5.1:+31356561 | None:intergenic |
| AGGTGCAGATACGGCAACTG+CGG | 0.650374 | 5.1:+31356600 | None:intergenic |
| AGACTAGGACTCCTTTCAAG+GGG | 0.655500 | 5.1:-31356444 | MS.gene76615:CDS |
| TAGGGATTCCATCGTCGTGG+AGG | 0.656879 | 5.1:+31356855 | None:intergenic |
| GTATCTGCACCTGCTTCTGG+AGG | 0.657705 | 5.1:-31356589 | MS.gene76615:CDS |
| GAAGAAAGTGACGATGACAT+GGG | 0.678372 | 5.1:-31356512 | MS.gene76615:CDS |
| GGATAGGGATTCCATCGTCG+TGG | 0.687033 | 5.1:+31356852 | None:intergenic |
| TGAAAAGAAAGAGAACGCCA+AGG | 0.690255 | 5.1:-31356534 | MS.gene76615:CDS |
| GACTAGGACTCCTTTCAAGG+GGG | 0.714058 | 5.1:-31356443 | MS.gene76615:CDS |
| ATCGTCGTGGAGGATCAAGG+TGG | 0.743990 | 5.1:+31356865 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTCTTTGTTACTGTTT+GGG | - | chr5.1:31356504-31356523 | MS.gene76615:CDS | 20.0% |
| ! | TACAGAAATCGAATTGAATA+GGG | + | chr5.1:31356460-31356479 | None:intergenic | 25.0% |
| ! | TTACAGAAATCGAATTGAAT+AGG | + | chr5.1:31356461-31356480 | None:intergenic | 25.0% |
| !! | CATTTTTCTTTGTTACTGTT+TGG | - | chr5.1:31356503-31356522 | MS.gene76615:CDS | 25.0% |
| ! | AGATAGTAGATTGCAAGTTT+TGG | + | chr5.1:31356863-31356882 | None:intergenic | 30.0% |
| !! | TACTGTTTGGGTTTTTGTTT+AGG | - | chr5.1:31356516-31356535 | MS.gene76615:CDS | 30.0% |
| AATGAGATCTATGAGCGATT+TGG | + | chr5.1:31356487-31356506 | None:intergenic | 35.0% | |
| TTTGTTTAGGCGAAGAAGAT+CGG | - | chr5.1:31356529-31356548 | MS.gene76615:CDS | 35.0% | |
| ! | GTTCTCTTTCTTTTCAACAG+CGG | + | chr5.1:31356733-31356752 | None:intergenic | 35.0% |
| AATAGGGATAGCAGACTTAC+GGG | + | chr5.1:31356444-31356463 | None:intergenic | 40.0% | |
| GAAGAAAGTGACGATGACAT+GGG | - | chr5.1:31356759-31356778 | MS.gene76615:intron | 40.0% | |
| GAAGATCGGTACTTTGTTGA+AGG | - | chr5.1:31356543-31356562 | MS.gene76615:CDS | 40.0% | |
| GAATAGGGATAGCAGACTTA+CGG | + | chr5.1:31356445-31356464 | None:intergenic | 40.0% | |
| TATGAGCAAGCTTAGCGAAT+AGG | + | chr5.1:31356603-31356622 | None:intergenic | 40.0% | |
| TGAAAAGAAAGAGAACGCCA+AGG | - | chr5.1:31356737-31356756 | MS.gene76615:intron | 40.0% | |
| !! | GATGATCTCGTTTTGAACTC+CGG | - | chr5.1:31356634-31356653 | MS.gene76615:CDS | 40.0% |
| !! | TAGTTTGTTGTAGCCAGACT+AGG | - | chr5.1:31356812-31356831 | MS.gene76615:intron | 40.0% |
| ACGTCACCGTTGAATCTTAC+TGG | - | chr5.1:31356572-31356591 | MS.gene76615:CDS | 45.0% | |
| AGACTAGGACTCCTTTCAAG+GGG | - | chr5.1:31356827-31356846 | MS.gene76615:intron | 45.0% | |
| AGATTCAACGGTGACGTTAG+CGG | + | chr5.1:31356569-31356588 | None:intergenic | 45.0% | |
| ATAGCAGACTTACGGGGATA+GGG | + | chr5.1:31356437-31356456 | None:intergenic | 45.0% | |
| ATAGGGATAGCAGACTTACG+GGG | + | chr5.1:31356443-31356462 | None:intergenic | 45.0% | |
| CAGACTAGGACTCCTTTCAA+GGG | - | chr5.1:31356826-31356845 | MS.gene76615:intron | 45.0% | |
| GGAAGAAAGTGACGATGACA+TGG | - | chr5.1:31356758-31356777 | MS.gene76615:intron | 45.0% | |
| ! | GTCATCGTCACTTTCTTCCT+TGG | + | chr5.1:31356757-31356776 | None:intergenic | 45.0% |
| ! | TCTTTTCAACAGCGGCAGTT+CGG | + | chr5.1:31356725-31356744 | None:intergenic | 45.0% |
| ACCGTTGAATCTTACTGGCC+AGG | - | chr5.1:31356577-31356596 | MS.gene76615:CDS | 50.0% | |
| CCAGACTAGGACTCCTTTCA+AGG | - | chr5.1:31356825-31356844 | MS.gene76615:intron | 50.0% | |
| CCTTGAAAGGAGTCCTAGTC+TGG | + | chr5.1:31356828-31356847 | None:intergenic | 50.0% | |
| GACTAGGACTCCTTTCAAGG+GGG | - | chr5.1:31356828-31356847 | MS.gene76615:intron | 50.0% | |
| GATAGCAGACTTACGGGGAT+AGG | + | chr5.1:31356438-31356457 | None:intergenic | 50.0% | |
| GCCTGGCCAGTAAGATTCAA+CGG | + | chr5.1:31356581-31356600 | None:intergenic | 50.0% | |
| TCCAGAAGCAGGTGCAGATA+CGG | + | chr5.1:31356683-31356702 | None:intergenic | 50.0% | |
| ACCTTGATCCTCCACGACGA+TGG | - | chr5.1:31356408-31356427 | MS.gene76615:CDS | 55.0% | |
| AGGTGCAGATACGGCAACTG+CGG | + | chr5.1:31356674-31356693 | None:intergenic | 55.0% | |
| ATCGTCGTGGAGGATCAAGG+TGG | + | chr5.1:31356409-31356428 | None:intergenic | 55.0% | |
| GATTCAGCCACCCCCTTGAA+AGG | + | chr5.1:31356841-31356860 | None:intergenic | 55.0% | |
| GCAAGCTTAGCGAATAGGCC+TGG | + | chr5.1:31356598-31356617 | None:intergenic | 55.0% | |
| GGATAGGGATTCCATCGTCG+TGG | + | chr5.1:31356422-31356441 | None:intergenic | 55.0% | |
| GTATCTGCACCTGCTTCTGG+AGG | - | chr5.1:31356682-31356701 | MS.gene76615:CDS | 55.0% | |
| TAGGACTCCTTTCAAGGGGG+TGG | - | chr5.1:31356831-31356850 | MS.gene76615:intron | 55.0% | |
| TAGGGATTCCATCGTCGTGG+AGG | + | chr5.1:31356419-31356438 | None:intergenic | 55.0% | |
| TCCATCGTCGTGGAGGATCA+AGG | + | chr5.1:31356412-31356431 | None:intergenic | 55.0% | |
| !! | GTTTTGAACTCCGGCGCTGT+TGG | - | chr5.1:31356643-31356662 | MS.gene76615:CDS | 55.0% |
| CGGCAGTTCGGCTACTGTTG+CGG | + | chr5.1:31356713-31356732 | None:intergenic | 60.0% | |
| GCCGTATCTGCACCTGCTTC+TGG | - | chr5.1:31356679-31356698 | MS.gene76615:CDS | 60.0% | |
| TTGAACTCCGGCGCTGTTGG+TGG | - | chr5.1:31356646-31356665 | MS.gene76615:CDS | 60.0% | |
| ! | TCTGCACCTGCTTCTGGAGG+TGG | - | chr5.1:31356685-31356704 | MS.gene76615:CDS | 60.0% |
| GCGGCTCCACCTCCAGAAGC+AGG | + | chr5.1:31356694-31356713 | None:intergenic | 70.0% | |
| TGCGGCACCACCAACAGCGC+CGG | + | chr5.1:31356656-31356675 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 31356375 | 31356918 | 31356375 | ID=MS.gene76615 |
| chr5.1 | mRNA | 31356375 | 31356918 | 31356375 | ID=MS.gene76615.t1;Parent=MS.gene76615 |
| chr5.1 | exon | 31356850 | 31356918 | 31356850 | ID=MS.gene76615.t1.exon1;Parent=MS.gene76615.t1 |
| chr5.1 | CDS | 31356850 | 31356918 | 31356850 | ID=cds.MS.gene76615.t1;Parent=MS.gene76615.t1 |
| chr5.1 | exon | 31356375 | 31356755 | 31356375 | ID=MS.gene76615.t1.exon2;Parent=MS.gene76615.t1 |
| chr5.1 | CDS | 31356375 | 31356755 | 31356375 | ID=cds.MS.gene76615.t1;Parent=MS.gene76615.t1 |
| Gene Sequence |
| Protein sequence |
