Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032942.t1 | XP_013464220.1 | 90.8 | 65 | 6 | 0 | 1 | 65 | 1 | 65 | 4.90E-24 | 120.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032942.t1 | P52855 | 61.9 | 63 | 24 | 0 | 1 | 63 | 1 | 63 | 3.2e-16 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032942.t1 | A0A072VJD3 | 90.8 | 65 | 6 | 0 | 1 | 65 | 1 | 65 | 3.5e-24 | 120.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene01952 | MS.gene032942 | PPI |
| MS.gene032942 | MS.gene78764 | PPI |
| MS.gene43071 | MS.gene032942 | PPI |
| MS.gene08521 | MS.gene032942 | PPI |
| MS.gene01425 | MS.gene032942 | PPI |
| MS.gene042719 | MS.gene032942 | PPI |
| MS.gene06833 | MS.gene032942 | PPI |
| MS.gene067331 | MS.gene032942 | PPI |
| MS.gene013506 | MS.gene032942 | PPI |
| MS.gene045633 | MS.gene032942 | PPI |
| MS.gene76615 | MS.gene032942 | PPI |
| MS.gene88792 | MS.gene032942 | PPI |
| MS.gene032942 | MS.gene91826 | PPI |
| MS.gene82009 | MS.gene032942 | PPI |
| MS.gene073078 | MS.gene032942 | PPI |
| MS.gene053952 | MS.gene032942 | PPI |
| MS.gene97876 | MS.gene032942 | PPI |
| MS.gene069339 | MS.gene032942 | PPI |
| MS.gene032942 | MS.gene35379 | PPI |
| MS.gene009874 | MS.gene032942 | PPI |
| MS.gene44900 | MS.gene032942 | PPI |
| MS.gene047578 | MS.gene032942 | PPI |
| MS.gene060483 | MS.gene032942 | PPI |
| MS.gene002442 | MS.gene032942 | PPI |
| MS.gene032942 | MS.gene01091 | PPI |
| MS.gene06617 | MS.gene032942 | PPI |
| MS.gene057237 | MS.gene032942 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032942.t1 | MTR_3g092600 | 90.164 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 1.87e-33 | 114 |
| MS.gene032942.t1 | MTR_2g066750 | 90.164 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 1.87e-33 | 114 |
| MS.gene032942.t1 | MTR_8g024350 | 64.384 | 73 | 25 | 1 | 1 | 73 | 1 | 72 | 3.07e-26 | 95.9 |
| MS.gene032942.t1 | MTR_7g118060 | 61.017 | 59 | 23 | 0 | 1 | 59 | 1 | 59 | 1.52e-19 | 78.6 |
| MS.gene032942.t1 | MTR_7g005740 | 58.065 | 62 | 7 | 1 | 1 | 62 | 1 | 43 | 9.25e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032942.t1 | AT5G24510 | 60.000 | 80 | 29 | 2 | 1 | 77 | 1 | 80 | 5.46e-25 | 92.8 |
| MS.gene032942.t1 | AT5G47700 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 3.46e-20 | 80.5 |
| MS.gene032942.t1 | AT5G47700 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 3.46e-20 | 80.5 |
| MS.gene032942.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 6.72e-20 | 79.3 |
| MS.gene032942.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.21e-19 | 79.0 |
| MS.gene032942.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.21e-19 | 79.0 |
| MS.gene032942.t1 | AT1G01100 | 58.333 | 60 | 25 | 0 | 2 | 61 | 3 | 62 | 1.21e-19 | 79.0 |
| MS.gene032942.t1 | AT4G00810 | 56.667 | 60 | 26 | 0 | 2 | 61 | 3 | 62 | 5.92e-19 | 77.4 |
| MS.gene032942.t1 | AT4G00810 | 56.667 | 60 | 26 | 0 | 2 | 61 | 3 | 62 | 5.92e-19 | 77.4 |
Find 38 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGTATCTGCACCTGCTTC+TGG | 0.262672 | 5.4:-28325730 | MS.gene032942:CDS |
| AGATAGTAGATTGCAAGTTT+TGG | 0.304388 | 5.4:+28325549 | None:intergenic |
| GTCATCGTCACTTTCTTCCT+TGG | 0.339396 | 5.4:+28325655 | None:intergenic |
| ACGTCACCGTTGAATCTTAC+TGG | 0.348075 | 5.4:-28325837 | MS.gene032942:CDS |
| CAAAATGAGTTTCGGCGAAT+CGG | 0.351766 | 5.4:-28326038 | None:intergenic |
| AAAATGAGTTTCGGCGAATC+GGG | 0.370184 | 5.4:-28326037 | MS.gene032942:CDS |
| ACAATTCACAAAATGAGTTT+CGG | 0.409091 | 5.4:-28326046 | None:intergenic |
| CCAGACTAGGACTCCTTTCA+AGG | 0.415086 | 5.4:-28325584 | MS.gene032942:CDS |
| CCTTGAAAGGAGTCCTAGTC+TGG | 0.418177 | 5.4:+28325584 | None:intergenic |
| TATGAGCAAGCTTAGCGAAT+AGG | 0.439056 | 5.4:+28325809 | None:intergenic |
| TCTGCACCTGCTTCTGGAGG+TGG | 0.452148 | 5.4:-28325724 | MS.gene032942:CDS |
| GATAGCAGACTTACGGGGAT+AGG | 0.472811 | 5.4:+28325974 | None:intergenic |
| GAAGATCGGTACTTTGTTGA+AGG | 0.484661 | 5.4:-28325866 | MS.gene032942:CDS |
| ACCGTTGAATCTTACTGGCC+AGG | 0.494210 | 5.4:-28325832 | MS.gene032942:CDS |
| GCGGCTCCACCTCCAGAAGC+AGG | 0.495490 | 5.4:+28325718 | None:intergenic |
| TCCAGAAGCAGGTGCAGATA+CGG | 0.506287 | 5.4:+28325729 | None:intergenic |
| TTGAACTCCGGCGCTGTTGG+TGG | 0.510539 | 5.4:-28325763 | MS.gene032942:CDS |
| ATAGCAGACTTACGGGGATA+GGG | 0.518236 | 5.4:+28325975 | None:intergenic |
| TAGTTTGTTGTAGCCAGACT+AGG | 0.521961 | 5.4:-28325597 | MS.gene032942:CDS |
| GGAAGAAAGTGACGATGACA+TGG | 0.523361 | 5.4:-28325651 | MS.gene032942:CDS |
| AGATTCAACGGTGACGTTAG+CGG | 0.526016 | 5.4:+28325843 | None:intergenic |
| TTTGTTTAGGCGAAGAAGAT+CGG | 0.532771 | 5.4:-28325880 | MS.gene032942:intron |
| TCCATCGTCGTGGAGGATCA+AGG | 0.532980 | 5.4:+28326000 | None:intergenic |
| GCAAGCTTAGCGAATAGGCC+TGG | 0.533765 | 5.4:+28325814 | None:intergenic |
| TGCGGCACCACCAACAGCGC+CGG | 0.552848 | 5.4:+28325756 | None:intergenic |
| ACCTTGATCCTCCACGACGA+TGG | 0.573503 | 5.4:-28326001 | MS.gene032942:CDS |
| CAGACTAGGACTCCTTTCAA+GGG | 0.583004 | 5.4:-28325583 | MS.gene032942:CDS |
| GCCTGGCCAGTAAGATTCAA+CGG | 0.587082 | 5.4:+28325831 | None:intergenic |
| CGGCAGTTCGGCTACTGTTG+CGG | 0.596856 | 5.4:+28325699 | None:intergenic |
| GTATCTGCACCTGCTTCTGG+AGG | 0.645858 | 5.4:-28325727 | MS.gene032942:CDS |
| AGACTAGGACTCCTTTCAAG+GGG | 0.655500 | 5.4:-28325582 | MS.gene032942:CDS |
| TAGGGATTCCATCGTCGTGG+AGG | 0.656879 | 5.4:+28325993 | None:intergenic |
| GAAGAAAGTGACGATGACAT+GGG | 0.678372 | 5.4:-28325650 | MS.gene032942:CDS |
| GGATAGGGATTCCATCGTCG+TGG | 0.687033 | 5.4:+28325990 | None:intergenic |
| TGAAAAGAAAGAGAACGCCA+AGG | 0.690255 | 5.4:-28325672 | MS.gene032942:CDS |
| GACTAGGACTCCTTTCAAGG+GGG | 0.714058 | 5.4:-28325581 | MS.gene032942:CDS |
| AGGTGCAGATACGGTAACTG+CGG | 0.716144 | 5.4:+28325738 | None:intergenic |
| ATCGTCGTGGAGGATCAAGG+TGG | 0.743990 | 5.4:+28326003 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTCTTTGTTACTGTTT+GGG | - | chr5.4:28325642-28325661 | MS.gene032942:CDS | 20.0% |
| ! | TACAGAAATCGAATTGAATA+GGG | + | chr5.4:28325598-28325617 | None:intergenic | 25.0% |
| ! | TTACAGAAATCGAATTGAAT+AGG | + | chr5.4:28325599-28325618 | None:intergenic | 25.0% |
| !! | CATTTTTCTTTGTTACTGTT+TGG | - | chr5.4:28325641-28325660 | MS.gene032942:CDS | 25.0% |
| ! | AGATAGTAGATTGCAAGTTT+TGG | + | chr5.4:28326001-28326020 | None:intergenic | 30.0% |
| !! | TACTGTTTGGGTTTTTGTTT+AGG | - | chr5.4:28325654-28325673 | MS.gene032942:CDS | 30.0% |
| AATGAGATCTATGAGCGATT+TGG | + | chr5.4:28325625-28325644 | None:intergenic | 35.0% | |
| TTTGTTTAGGCGAAGAAGAT+CGG | - | chr5.4:28325667-28325686 | MS.gene032942:CDS | 35.0% | |
| ! | GTTCTCTTTCTTTTCAACAG+CGG | + | chr5.4:28325871-28325890 | None:intergenic | 35.0% |
| AATAGGGATAGCAGACTTAC+GGG | + | chr5.4:28325582-28325601 | None:intergenic | 40.0% | |
| GAAGAAAGTGACGATGACAT+GGG | - | chr5.4:28325897-28325916 | MS.gene032942:intron | 40.0% | |
| GAAGATCGGTACTTTGTTGA+AGG | - | chr5.4:28325681-28325700 | MS.gene032942:CDS | 40.0% | |
| GAATAGGGATAGCAGACTTA+CGG | + | chr5.4:28325583-28325602 | None:intergenic | 40.0% | |
| TATGAGCAAGCTTAGCGAAT+AGG | + | chr5.4:28325741-28325760 | None:intergenic | 40.0% | |
| TGAAAAGAAAGAGAACGCCA+AGG | - | chr5.4:28325875-28325894 | MS.gene032942:intron | 40.0% | |
| !! | GATGATCTCGTTTTGAACTC+CGG | - | chr5.4:28325772-28325791 | MS.gene032942:CDS | 40.0% |
| !! | TAGTTTGTTGTAGCCAGACT+AGG | - | chr5.4:28325950-28325969 | MS.gene032942:intron | 40.0% |
| ACGTCACCGTTGAATCTTAC+TGG | - | chr5.4:28325710-28325729 | MS.gene032942:CDS | 45.0% | |
| AGACTAGGACTCCTTTCAAG+GGG | - | chr5.4:28325965-28325984 | MS.gene032942:intron | 45.0% | |
| AGATTCAACGGTGACGTTAG+CGG | + | chr5.4:28325707-28325726 | None:intergenic | 45.0% | |
| ATAGCAGACTTACGGGGATA+GGG | + | chr5.4:28325575-28325594 | None:intergenic | 45.0% | |
| ATAGGGATAGCAGACTTACG+GGG | + | chr5.4:28325581-28325600 | None:intergenic | 45.0% | |
| CAGACTAGGACTCCTTTCAA+GGG | - | chr5.4:28325964-28325983 | MS.gene032942:intron | 45.0% | |
| GGAAGAAAGTGACGATGACA+TGG | - | chr5.4:28325896-28325915 | MS.gene032942:intron | 45.0% | |
| ! | GTCATCGTCACTTTCTTCCT+TGG | + | chr5.4:28325895-28325914 | None:intergenic | 45.0% |
| ! | TCTTTTCAACAGCGGCAGTT+CGG | + | chr5.4:28325863-28325882 | None:intergenic | 45.0% |
| ACCGTTGAATCTTACTGGCC+AGG | - | chr5.4:28325715-28325734 | MS.gene032942:CDS | 50.0% | |
| AGGTGCAGATACGGTAACTG+CGG | + | chr5.4:28325812-28325831 | None:intergenic | 50.0% | |
| CCAGACTAGGACTCCTTTCA+AGG | - | chr5.4:28325963-28325982 | MS.gene032942:intron | 50.0% | |
| CCTTGAAAGGAGTCCTAGTC+TGG | + | chr5.4:28325966-28325985 | None:intergenic | 50.0% | |
| GACTAGGACTCCTTTCAAGG+GGG | - | chr5.4:28325966-28325985 | MS.gene032942:intron | 50.0% | |
| GATAGCAGACTTACGGGGAT+AGG | + | chr5.4:28325576-28325595 | None:intergenic | 50.0% | |
| GCCTGGCCAGTAAGATTCAA+CGG | + | chr5.4:28325719-28325738 | None:intergenic | 50.0% | |
| TCCAGAAGCAGGTGCAGATA+CGG | + | chr5.4:28325821-28325840 | None:intergenic | 50.0% | |
| ACCGTATCTGCACCTGCTTC+TGG | - | chr5.4:28325817-28325836 | MS.gene032942:CDS | 55.0% | |
| ACCTTGATCCTCCACGACGA+TGG | - | chr5.4:28325546-28325565 | MS.gene032942:CDS | 55.0% | |
| ATCGTCGTGGAGGATCAAGG+TGG | + | chr5.4:28325547-28325566 | None:intergenic | 55.0% | |
| GATTCAGCCACCCCCTTGAA+AGG | + | chr5.4:28325979-28325998 | None:intergenic | 55.0% | |
| GCAAGCTTAGCGAATAGGCC+TGG | + | chr5.4:28325736-28325755 | None:intergenic | 55.0% | |
| GGATAGGGATTCCATCGTCG+TGG | + | chr5.4:28325560-28325579 | None:intergenic | 55.0% | |
| GTATCTGCACCTGCTTCTGG+AGG | - | chr5.4:28325820-28325839 | MS.gene032942:CDS | 55.0% | |
| TAGGACTCCTTTCAAGGGGG+TGG | - | chr5.4:28325969-28325988 | MS.gene032942:intron | 55.0% | |
| TAGGGATTCCATCGTCGTGG+AGG | + | chr5.4:28325557-28325576 | None:intergenic | 55.0% | |
| TCCATCGTCGTGGAGGATCA+AGG | + | chr5.4:28325550-28325569 | None:intergenic | 55.0% | |
| !! | GTTTTGAACTCCGGCGCTGT+TGG | - | chr5.4:28325781-28325800 | MS.gene032942:CDS | 55.0% |
| CGGCAGTTCGGCTACTGTTG+CGG | + | chr5.4:28325851-28325870 | None:intergenic | 60.0% | |
| TTGAACTCCGGCGCTGTTGG+TGG | - | chr5.4:28325784-28325803 | MS.gene032942:CDS | 60.0% | |
| ! | TCTGCACCTGCTTCTGGAGG+TGG | - | chr5.4:28325823-28325842 | MS.gene032942:CDS | 60.0% |
| GCGGCTCCACCTCCAGAAGC+AGG | + | chr5.4:28325832-28325851 | None:intergenic | 70.0% | |
| TGCGGCACCACCAACAGCGC+CGG | + | chr5.4:28325794-28325813 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 28325513 | 28326056 | 28325513 | ID=MS.gene032942 |
| chr5.4 | mRNA | 28325513 | 28326056 | 28325513 | ID=MS.gene032942.t1;Parent=MS.gene032942 |
| chr5.4 | exon | 28325988 | 28326056 | 28325988 | ID=MS.gene032942.t1.exon1;Parent=MS.gene032942.t1 |
| chr5.4 | CDS | 28325988 | 28326056 | 28325988 | ID=cds.MS.gene032942.t1;Parent=MS.gene032942.t1 |
| chr5.4 | exon | 28325513 | 28325893 | 28325513 | ID=MS.gene032942.t1.exon2;Parent=MS.gene032942.t1 |
| chr5.4 | CDS | 28325513 | 28325893 | 28325513 | ID=cds.MS.gene032942.t1;Parent=MS.gene032942.t1 |
| Gene Sequence |
| Protein sequence |
