Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78764.t1 | XP_013442308.1 | 93.9 | 66 | 4 | 0 | 1 | 66 | 1 | 66 | 3.10E-23 | 117.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78764.t1 | Q9SLF7 | 75.8 | 66 | 16 | 0 | 1 | 66 | 1 | 66 | 1.4e-19 | 97.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78764.t1 | B7FMX7 | 93.9 | 66 | 4 | 0 | 1 | 66 | 1 | 66 | 2.3e-23 | 117.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050916 | MS.gene78764 | 0.824232 | 9.02E-54 | -1.69E-46 |
| MS.gene051903 | MS.gene78764 | 0.811116 | 8.22E-51 | -1.69E-46 |
| MS.gene053952 | MS.gene78764 | 0.85526 | 7.11E-62 | -1.69E-46 |
| MS.gene056075 | MS.gene78764 | 0.810977 | 8.81E-51 | -1.69E-46 |
| MS.gene058354 | MS.gene78764 | 0.804993 | 1.65E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene78764 | 0.833187 | 6.16E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene032942 | MS.gene78764 | PPI |
| MS.gene050761 | MS.gene78764 | PPI |
| MS.gene052265 | MS.gene78764 | PPI |
| MS.gene78764 | MS.gene91826 | PPI |
| MS.gene009874 | MS.gene78764 | PPI |
| MS.gene44900 | MS.gene78764 | PPI |
| MS.gene91826 | MS.gene78764 | PPI |
| MS.gene053952 | MS.gene78764 | PPI |
| MS.gene78764 | MS.gene35379 | PPI |
| MS.gene003413 | MS.gene78764 | PPI |
| MS.gene28964 | MS.gene78764 | PPI |
| MS.gene072563 | MS.gene78764 | PPI |
| MS.gene28704 | MS.gene78764 | PPI |
| MS.gene052268 | MS.gene78764 | PPI |
| MS.gene054381 | MS.gene78764 | PPI |
| MS.gene050964 | MS.gene78764 | PPI |
| MS.gene060483 | MS.gene78764 | PPI |
| MS.gene06617 | MS.gene78764 | PPI |
| MS.gene01952 | MS.gene78764 | PPI |
| MS.gene43071 | MS.gene78764 | PPI |
| MS.gene08521 | MS.gene78764 | PPI |
| MS.gene042719 | MS.gene78764 | PPI |
| MS.gene06833 | MS.gene78764 | PPI |
| MS.gene067331 | MS.gene78764 | PPI |
| MS.gene013506 | MS.gene78764 | PPI |
| MS.gene35379 | MS.gene78764 | PPI |
| MS.gene76615 | MS.gene78764 | PPI |
| MS.gene057237 | MS.gene78764 | PPI |
| MS.gene88792 | MS.gene78764 | PPI |
| MS.gene82009 | MS.gene78764 | PPI |
| MS.gene073078 | MS.gene78764 | PPI |
| MS.gene045633 | MS.gene78764 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78764.t1 | MTR_0227s0020 | 96.460 | 113 | 4 | 0 | 1 | 113 | 1 | 113 | 7.83e-71 | 207 |
| MS.gene78764.t1 | MTR_2g085085 | 89.381 | 113 | 12 | 0 | 1 | 113 | 1 | 113 | 1.22e-45 | 143 |
| MS.gene78764.t1 | MTR_1g060600 | 73.451 | 113 | 29 | 1 | 1 | 113 | 1 | 112 | 4.45e-28 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78764.t1 | AT2G27710 | 77.143 | 70 | 16 | 0 | 1 | 70 | 1 | 70 | 3.52e-32 | 108 |
| MS.gene78764.t1 | AT3G44590 | 65.487 | 113 | 37 | 2 | 1 | 113 | 1 | 111 | 7.62e-31 | 106 |
| MS.gene78764.t1 | AT3G44590 | 65.487 | 113 | 37 | 2 | 1 | 113 | 1 | 111 | 7.62e-31 | 106 |
| MS.gene78764.t1 | AT2G27720 | 66.087 | 115 | 37 | 1 | 1 | 113 | 1 | 115 | 4.70e-28 | 99.4 |
| MS.gene78764.t1 | AT2G27710 | 75.385 | 65 | 16 | 0 | 1 | 65 | 1 | 65 | 1.72e-25 | 92.8 |
| MS.gene78764.t1 | AT2G27710 | 75.385 | 65 | 16 | 0 | 1 | 65 | 1 | 65 | 1.72e-25 | 92.8 |
| MS.gene78764.t1 | AT2G27710 | 75.385 | 65 | 16 | 0 | 1 | 65 | 1 | 65 | 1.72e-25 | 92.8 |
| MS.gene78764.t1 | AT2G27720 | 59.843 | 127 | 37 | 2 | 1 | 113 | 1 | 127 | 1.82e-24 | 90.5 |
| MS.gene78764.t1 | AT3G28500 | 55.556 | 117 | 46 | 4 | 1 | 113 | 1 | 115 | 3.59e-24 | 89.4 |
| MS.gene78764.t1 | AT2G27720 | 57.692 | 130 | 38 | 2 | 1 | 113 | 1 | 130 | 2.99e-23 | 87.4 |
| MS.gene78764.t1 | AT2G27720 | 66.667 | 87 | 27 | 1 | 29 | 113 | 5 | 91 | 2.48e-14 | 63.5 |
| MS.gene78764.t1 | AT5G40040 | 57.018 | 114 | 48 | 1 | 1 | 113 | 1 | 114 | 8.82e-14 | 62.8 |
Find 27 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTATTTGTTGGCTGTTT+TGG | 0.077686 | 5.1:+40902304 | MS.gene78764:CDS |
| TCCTCCTTTGCTTCGGCAGC+TGG | 0.312544 | 5.1:-40903085 | None:intergenic |
| AAGTTGGCTTCAGTGCCTTC+TGG | 0.324137 | 5.1:+40903007 | MS.gene78764:CDS |
| GCTGTGGCTGTTGCTGCTTC+CGG | 0.372107 | 5.1:+40903037 | MS.gene78764:CDS |
| GGAAAAGAAGGTTGAAGAAA+AGG | 0.373370 | 5.1:+40903105 | MS.gene78764:CDS |
| GATCATCGCAGCTTATTTGT+TGG | 0.413285 | 5.1:+40902295 | MS.gene78764:CDS |
| GCTGTTGCTGCTTCCGGTGG+AGG | 0.434566 | 5.1:+40903043 | MS.gene78764:CDS |
| GCTTCAGTGCCTTCTGGTGG+AGG | 0.435432 | 5.1:+40903013 | MS.gene78764:CDS |
| AGCAGCTGCAGCACCTCCAC+CGG | 0.469171 | 5.1:-40903056 | None:intergenic |
| CAACTCAACATTTGAGTCCT+CGG | 0.476762 | 5.1:-40902927 | None:intergenic |
| ATCAAAACCATCCTCGCCTC+AGG | 0.486163 | 5.1:+40902356 | MS.gene78764:CDS |
| TGCTAGTGGAAGAGAGAAGT+TGG | 0.493210 | 5.1:+40902991 | MS.gene78764:CDS |
| TGCACCAGCTGCCGAAGCAA+AGG | 0.494374 | 5.1:+40903081 | MS.gene78764:CDS |
| GCCTTCTGGTGGAGGTGCTG+TGG | 0.497647 | 5.1:+40903021 | MS.gene78764:CDS |
| TTGGCTTCAGTGCCTTCTGG+TGG | 0.507222 | 5.1:+40903010 | MS.gene78764:CDS |
| GCCACAGCACCTCCACCAGA+AGG | 0.515129 | 5.1:-40903022 | None:intergenic |
| TTTGATATCATCAGCGGAAG+GGG | 0.519904 | 5.1:-40902339 | None:intergenic |
| AACAAAGAAACTTACATCAT+CGG | 0.541190 | 5.1:-40903134 | None:intergenic |
| CGAAGCAAAGGAGGAAAAGA+AGG | 0.550592 | 5.1:+40903093 | MS.gene78764:CDS |
| GTAAGAACCTGAGGCGAGGA+TGG | 0.551127 | 5.1:-40902363 | None:intergenic |
| GTGGCTGTTGCTGCTTCCGG+TGG | 0.555914 | 5.1:+40903040 | MS.gene78764:CDS |
| TTTGAGTGAAGTCAAGGGCA+AGG | 0.559487 | 5.1:+40902952 | MS.gene78764:CDS |
| ACCAGCTGCCGAAGCAAAGG+AGG | 0.585812 | 5.1:+40903084 | MS.gene78764:CDS |
| TTTGCCGAGCTAATTGCTAG+TGG | 0.606320 | 5.1:+40902977 | MS.gene78764:CDS |
| TCTTCCACTAGCAATTAGCT+CGG | 0.634325 | 5.1:-40902981 | None:intergenic |
| AGAGGTAAGAACCTGAGGCG+AGG | 0.656993 | 5.1:-40902367 | None:intergenic |
| TATAGTTGGAGTTGAAGCCG+AGG | 0.682665 | 5.1:+40902910 | MS.gene78764:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGTTTATATATATTCTT+CGG | + | chr5.1:40902801-40902820 | MS.gene78764:intron | 10.0% |
| !! | AACAATAAATAAATTTGTCA+AGG | - | chr5.1:40902773-40902792 | None:intergenic | 15.0% |
| !!! | TTCTTTGTTATTTTTATAGT+TGG | + | chr5.1:40902896-40902915 | MS.gene78764:intron | 15.0% |
| !! | ATCAAAATTCAATGTTTATC+AGG | - | chr5.1:40902746-40902765 | None:intergenic | 20.0% |
| !! | CAATAATCAAAATCTATGAA+CGG | - | chr5.1:40902696-40902715 | None:intergenic | 20.0% |
| !! | TTACTACATTGAATTGTTAT+CGG | + | chr5.1:40902635-40902654 | MS.gene78764:intron | 20.0% |
| !! | TTGATTGTAATTGAATATAG+TGG | + | chr5.1:40902519-40902538 | MS.gene78764:intron | 20.0% |
| ! | AAACATAACCAAAAAAAGAG+AGG | - | chr5.1:40902388-40902407 | None:intergenic | 25.0% |
| ! | AACAAAAAAATTGCAAAGAG+TGG | - | chr5.1:40902555-40902574 | None:intergenic | 25.0% |
| ! | AACAAAGAAACTTACATCAT+CGG | - | chr5.1:40903137-40903156 | None:intergenic | 25.0% |
| ! | GTTATGTAGTTGAATGAATT+AGG | + | chr5.1:40902495-40902514 | MS.gene78764:intron | 25.0% |
| AATGTTTATCAGGCTTCAAA+AGG | - | chr5.1:40902736-40902755 | None:intergenic | 30.0% | |
| GGAAAAGAAGGTTGAAGAAA+AGG | + | chr5.1:40903105-40903124 | MS.gene78764:CDS | 35.0% | |
| ! | TTTTGATATCATCAGCGGAA+GGG | - | chr5.1:40902343-40902362 | None:intergenic | 35.0% |
| !! | AGCTTATTTGTTGGCTGTTT+TGG | + | chr5.1:40902304-40902323 | MS.gene78764:CDS | 35.0% |
| !! | GCTTATTTGTTGGCTGTTTT+GGG | + | chr5.1:40902305-40902324 | MS.gene78764:CDS | 35.0% |
| !! | TTGCTTTTGAGTGAAGTCAA+GGG | + | chr5.1:40902947-40902966 | MS.gene78764:CDS | 35.0% |
| !!! | ATCTTTGTTTTGCAGGACAT+GGG | + | chr5.1:40903218-40903237 | MS.gene78764:intron | 35.0% |
| !!! | GATGGTTTTGATATCATCAG+CGG | - | chr5.1:40902348-40902367 | None:intergenic | 35.0% |
| !!! | GGTTCTTACCTCTCTTTTTT+TGG | + | chr5.1:40902377-40902396 | MS.gene78764:intron | 35.0% |
| AAAAGAGAGGTAAGAACCTG+AGG | - | chr5.1:40902375-40902394 | None:intergenic | 40.0% | |
| CAAAATCTATGAACGGACAC+CGG | - | chr5.1:40902689-40902708 | None:intergenic | 40.0% | |
| CAACTCAACATTTGAGTCCT+CGG | - | chr5.1:40902930-40902949 | None:intergenic | 40.0% | |
| GATCATCGCAGCTTATTTGT+TGG | + | chr5.1:40902295-40902314 | MS.gene78764:CDS | 40.0% | |
| TCTTCCACTAGCAATTAGCT+CGG | - | chr5.1:40902984-40903003 | None:intergenic | 40.0% | |
| TTTGATATCATCAGCGGAAG+GGG | - | chr5.1:40902342-40902361 | None:intergenic | 40.0% | |
| ! | CTTCTTTTCCTCCTTTGCTT+CGG | - | chr5.1:40903095-40903114 | None:intergenic | 40.0% |
| !! | GTTTTGATATCATCAGCGGA+AGG | - | chr5.1:40902344-40902363 | None:intergenic | 40.0% |
| !! | TATTTGTTGGCTGTTTTGGG+CGG | + | chr5.1:40902308-40902327 | MS.gene78764:CDS | 40.0% |
| !!! | CATCTTTGTTTTGCAGGACA+TGG | + | chr5.1:40903217-40903236 | MS.gene78764:intron | 40.0% |
| !!! | CGTTGTCATCTTTGTTTTGC+AGG | + | chr5.1:40903211-40903230 | MS.gene78764:intron | 40.0% |
| !!! | GTTGCTTTTGAGTGAAGTCA+AGG | + | chr5.1:40902946-40902965 | MS.gene78764:CDS | 40.0% |
| CGAAGCAAAGGAGGAAAAGA+AGG | + | chr5.1:40903093-40903112 | MS.gene78764:CDS | 45.0% | |
| TATAGTTGGAGTTGAAGCCG+AGG | + | chr5.1:40902910-40902929 | MS.gene78764:intron | 45.0% | |
| TGCTAGTGGAAGAGAGAAGT+TGG | + | chr5.1:40902991-40903010 | MS.gene78764:CDS | 45.0% | |
| TTTGAGTGAAGTCAAGGGCA+AGG | + | chr5.1:40902952-40902971 | MS.gene78764:CDS | 45.0% | |
| TTTGCCGAGCTAATTGCTAG+TGG | + | chr5.1:40902977-40902996 | MS.gene78764:CDS | 45.0% | |
| !! | GTTATCGGTTTCGATTTGCC+TGG | + | chr5.1:40902650-40902669 | MS.gene78764:intron | 45.0% |
| ATCAAAACCATCCTCGCCTC+AGG | + | chr5.1:40902356-40902375 | MS.gene78764:CDS | 50.0% | |
| ATCTATGAACGGACACCGGA+CGG | - | chr5.1:40902685-40902704 | None:intergenic | 50.0% | |
| GCTTTCGCTCACTTCTTCAG+AGG | - | chr5.1:40902835-40902854 | None:intergenic | 50.0% | |
| ! | AAGTTGGCTTCAGTGCCTTC+TGG | + | chr5.1:40903007-40903026 | MS.gene78764:CDS | 50.0% |
| AGAGGTAAGAACCTGAGGCG+AGG | - | chr5.1:40902370-40902389 | None:intergenic | 55.0% | |
| GTAAGAACCTGAGGCGAGGA+TGG | - | chr5.1:40902366-40902385 | None:intergenic | 55.0% | |
| TTGGCTTCAGTGCCTTCTGG+TGG | + | chr5.1:40903010-40903029 | MS.gene78764:CDS | 55.0% | |
| ACCAGCTGCCGAAGCAAAGG+AGG | + | chr5.1:40903084-40903103 | MS.gene78764:CDS | 60.0% | |
| ACCGGACGGACACAGATACC+AGG | - | chr5.1:40902671-40902690 | None:intergenic | 60.0% | |
| GCCTGGTATCTGTGTCCGTC+CGG | + | chr5.1:40902667-40902686 | MS.gene78764:intron | 60.0% | |
| GCTTCAGTGCCTTCTGGTGG+AGG | + | chr5.1:40903013-40903032 | MS.gene78764:CDS | 60.0% | |
| TCCTCCTTTGCTTCGGCAGC+TGG | - | chr5.1:40903088-40903107 | None:intergenic | 60.0% | |
| TGCACCAGCTGCCGAAGCAA+AGG | + | chr5.1:40903081-40903100 | MS.gene78764:CDS | 60.0% | |
| ! | GCTGTGGCTGTTGCTGCTTC+CGG | + | chr5.1:40903037-40903056 | MS.gene78764:CDS | 60.0% |
| AGCAGCTGCAGCACCTCCAC+CGG | - | chr5.1:40903059-40903078 | None:intergenic | 65.0% | |
| GCCACAGCACCTCCACCAGA+AGG | - | chr5.1:40903025-40903044 | None:intergenic | 65.0% | |
| ! | GCTGTTGCTGCTTCCGGTGG+AGG | + | chr5.1:40903043-40903062 | MS.gene78764:CDS | 65.0% |
| ! | GTGGCTGTTGCTGCTTCCGG+TGG | + | chr5.1:40903040-40903059 | MS.gene78764:CDS | 65.0% |
| !! | GCCTTCTGGTGGAGGTGCTG+TGG | + | chr5.1:40903021-40903040 | MS.gene78764:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 40902290 | 40903259 | 40902290 | ID=MS.gene78764 |
| chr5.1 | mRNA | 40902290 | 40903259 | 40902290 | ID=MS.gene78764.t1;Parent=MS.gene78764 |
| chr5.1 | exon | 40902290 | 40902377 | 40902290 | ID=MS.gene78764.t1.exon1;Parent=MS.gene78764.t1 |
| chr5.1 | CDS | 40902290 | 40902377 | 40902290 | ID=cds.MS.gene78764.t1;Parent=MS.gene78764.t1 |
| chr5.1 | exon | 40902915 | 40903141 | 40902915 | ID=MS.gene78764.t1.exon2;Parent=MS.gene78764.t1 |
| chr5.1 | CDS | 40902915 | 40903141 | 40902915 | ID=cds.MS.gene78764.t1;Parent=MS.gene78764.t1 |
| chr5.1 | exon | 40903233 | 40903259 | 40903233 | ID=MS.gene78764.t1.exon3;Parent=MS.gene78764.t1 |
| chr5.1 | CDS | 40903233 | 40903259 | 40903233 | ID=cds.MS.gene78764.t1;Parent=MS.gene78764.t1 |
| Gene Sequence |
| Protein sequence |
