AlfalfaGEDB | MS.gene042719

M. sativa cultivar XinJiangDaYe / MS.gene042719

KEGG: K02943
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006414 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044249 GO:0044260 GO:0071704 GO:0110165 GO:1901576
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042719.t1	XP_013442308.1	93.9	66	4	0	1	66	1	66	3.10E-23	117.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042719.t1	Q9SLF7	75.8	66	16	0	1	66	1	66	1.4e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042719.t1	B7FMX7	93.9	66	4	0	1	66	1	66	2.3e-23	117.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene042719	MS.gene44900	PPI
MS.gene042719	MS.gene013506	PPI
MS.gene042719	MS.gene003413	PPI
MS.gene042719	MS.gene009874	PPI
MS.gene042719	MS.gene032942	PPI
MS.gene042719	MS.gene06617	PPI
MS.gene073078	MS.gene042719	PPI
MS.gene042719	MS.gene88792	PPI
MS.gene042719	MS.gene43071	PPI
MS.gene042719	MS.gene28704	PPI
MS.gene042719	MS.gene28964	PPI
MS.gene042719	MS.gene073078	PPI
MS.gene042719	MS.gene045633	PPI
MS.gene042719	MS.gene057237	PPI
MS.gene042719	MS.gene050964	PPI
MS.gene042719	MS.gene060483	PPI
MS.gene042719	MS.gene052265	PPI
MS.gene042719	MS.gene050761	PPI
MS.gene042719	MS.gene91826	PPI
MS.gene042719	MS.gene78764	PPI
MS.gene042719	MS.gene053952	PPI
MS.gene042719	MS.gene08521	PPI
MS.gene042719	MS.gene072563	PPI
MS.gene042719	MS.gene82009	PPI
MS.gene042719	MS.gene052268	PPI
MS.gene43071	MS.gene042719	PPI
MS.gene042719	MS.gene067331	PPI
MS.gene042719	MS.gene76615	PPI
MS.gene042719	MS.gene35379	PPI
MS.gene042719	MS.gene01952	PPI
MS.gene042719	MS.gene054381	PPI
MS.gene042719	MS.gene06833	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042719.t1	MTR_0227s0020	96.460	113	4	0	1	113	1	113	7.83e-71	207
MS.gene042719.t1	MTR_2g085085	89.381	113	12	0	1	113	1	113	1.22e-45	143
MS.gene042719.t1	MTR_1g060600	73.451	113	29	1	1	113	1	112	4.45e-28	99.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042719.t1	AT2G27710	77.143	70	16	0	1	70	1	70	3.52e-32	108
MS.gene042719.t1	AT3G44590	65.487	113	37	2	1	113	1	111	7.62e-31	106
MS.gene042719.t1	AT3G44590	65.487	113	37	2	1	113	1	111	7.62e-31	106
MS.gene042719.t1	AT2G27720	66.087	115	37	1	1	113	1	115	4.70e-28	99.4
MS.gene042719.t1	AT2G27710	75.385	65	16	0	1	65	1	65	1.72e-25	92.8
MS.gene042719.t1	AT2G27710	75.385	65	16	0	1	65	1	65	1.72e-25	92.8
MS.gene042719.t1	AT2G27710	75.385	65	16	0	1	65	1	65	1.72e-25	92.8
MS.gene042719.t1	AT2G27720	59.843	127	37	2	1	113	1	127	1.82e-24	90.5
MS.gene042719.t1	AT3G28500	55.556	117	46	4	1	113	1	115	3.59e-24	89.4
MS.gene042719.t1	AT2G27720	57.692	130	38	2	1	113	1	130	2.99e-23	87.4
MS.gene042719.t1	AT2G27720	66.667	87	27	1	29	113	5	91	2.48e-14	63.5
MS.gene042719.t1	AT5G40040	57.018	114	48	1	1	113	1	114	8.82e-14	62.8

Find 28 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTTATTTGTTGGCTGTTT+TGG	0.077686	5.2:+43293575	MS.gene042719:CDS
TCCTCCTTTGCTTCGGCAGC+TGG	0.312544	5.2:-43294322	None:intergenic
GCTGTGGCTGTTGCTGCTTC+CGG	0.372107	5.2:+43294274	MS.gene042719:CDS
GGAAAAGAAGGTTGAAGAAA+AGG	0.373370	5.2:+43294342	MS.gene042719:CDS
GATCATCGCAGCTTATTTGT+TGG	0.413285	5.2:+43293566	MS.gene042719:CDS
GCTGTTGCTGCTTCCGGTGG+AGG	0.434566	5.2:+43294280	MS.gene042719:CDS
TGCTAGTGGCAGAGAGAAGT+TGG	0.462166	5.2:+43294228	MS.gene042719:CDS
AGCAGCTGCAGCACCTCCAC+CGG	0.469171	5.2:-43294293	None:intergenic
CAACTCAACATTTGAGTCCT+CGG	0.476762	5.2:-43294164	None:intergenic
ATCAAAACCATCCTCGCCTC+AGG	0.486163	5.2:+43293627	MS.gene042719:CDS
TGCACCAGCTGCCGAAGCAA+AGG	0.494374	5.2:+43294318	MS.gene042719:CDS
AAGTTGGCTTCAGTGCCATC+TGG	0.501913	5.2:+43294244	MS.gene042719:CDS
GGGATTTAGCTTGTTCGACT+AGG	0.506077	5.2:+43294475	MS.gene042719:CDS
GCCATCTGGTGGAGGTGCTG+TGG	0.508789	5.2:+43294258	MS.gene042719:CDS
TTTGATATCATCAGCGGAAG+GGG	0.519904	5.2:-43293610	None:intergenic
AACAAAGAAACTTACATCAT+CGG	0.541190	5.2:-43294371	None:intergenic
GCTTCAGTGCCATCTGGTGG+AGG	0.543882	5.2:+43294250	MS.gene042719:CDS
CGAAGCAAAGGAGGAAAAGA+AGG	0.550592	5.2:+43294330	MS.gene042719:CDS
GTAAGAACCTGAGGCGAGGA+TGG	0.551127	5.2:-43293634	None:intergenic
TTGGCTTCAGTGCCATCTGG+TGG	0.551596	5.2:+43294247	MS.gene042719:CDS
GTGGCTGTTGCTGCTTCCGG+TGG	0.555914	5.2:+43294277	MS.gene042719:CDS
TTTGAGTGAAGTCAAGGGCA+AGG	0.559487	5.2:+43294189	MS.gene042719:CDS
ACCAGCTGCCGAAGCAAAGG+AGG	0.585812	5.2:+43294321	MS.gene042719:CDS
GCCACAGCACCTCCACCAGA+TGG	0.592642	5.2:-43294259	None:intergenic
TCTGCCACTAGCAATTAGCT+CGG	0.640840	5.2:-43294218	None:intergenic
TTTGCCGAGCTAATTGCTAG+TGG	0.651963	5.2:+43294214	MS.gene042719:CDS
AGAGGTAAGAACCTGAGGCG+AGG	0.656993	5.2:-43293638	None:intergenic
TATAGTTGGAGTTGAAGCCG+AGG	0.682665	5.2:+43294147	MS.gene042719:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTTGTTATTTTTATAGT+TGG	+	chr5.2:43294133-43294152	MS.gene042719:intron	15.0%
!!	ATCAAAATTCAATGTTTATC+AGG	-	chr5.2:43293985-43294004	None:intergenic	20.0%
!!	TTGATTGTAATTGAATATAG+TGG	+	chr5.2:43293793-43293812	MS.gene042719:intron	20.0%
!!!	TTTCTACATTGAATTGTTTT+CGG	+	chr5.2:43293909-43293928	MS.gene042719:intron	20.0%
!	AAACAAAAAATTGCAAAGAG+TGG	-	chr5.2:43293829-43293848	None:intergenic	25.0%
!	AAACATAACCAAAAAAAGAG+AGG	-	chr5.2:43293659-43293678	None:intergenic	25.0%
!	AACAAAGAAACTTACATCAT+CGG	-	chr5.2:43294374-43294393	None:intergenic	25.0%
!	GCTTCAATAATCAAAATCTA+CGG	-	chr5.2:43293963-43293982	None:intergenic	25.0%
!	GTTATGTAGTTGAATGAATT+AGG	+	chr5.2:43293769-43293788	MS.gene042719:intron	25.0%
!!	ATCCTTGACAAATCAATTTT+TGG	+	chr5.2:43294007-43294026	MS.gene042719:intron	25.0%
!!	GACCAAAAATTGATTTGTCA+AGG	-	chr5.2:43294012-43294031	None:intergenic	30.0%
	GGAAAAGAAGGTTGAAGAAA+AGG	+	chr5.2:43294342-43294361	MS.gene042719:CDS	35.0%
!	TTTTGATATCATCAGCGGAA+GGG	-	chr5.2:43293614-43293633	None:intergenic	35.0%
!!	AGCTTATTTGTTGGCTGTTT+TGG	+	chr5.2:43293575-43293594	MS.gene042719:CDS	35.0%
!!	GCTTATTTGTTGGCTGTTTT+GGG	+	chr5.2:43293576-43293595	MS.gene042719:CDS	35.0%
!!	TTGCTTTTGAGTGAAGTCAA+GGG	+	chr5.2:43294184-43294203	MS.gene042719:CDS	35.0%
!!!	ATCTTTGTTTTGCAGGACAT+GGG	+	chr5.2:43294455-43294474	MS.gene042719:intron	35.0%
!!!	CATTGTCATCTTTGTTTTGC+AGG	+	chr5.2:43294448-43294467	MS.gene042719:intron	35.0%
!!!	GATGGTTTTGATATCATCAG+CGG	-	chr5.2:43293619-43293638	None:intergenic	35.0%
!!!	GGTTCTTACCTCTCTTTTTT+TGG	+	chr5.2:43293648-43293667	MS.gene042719:intron	35.0%
	AAAAGAGAGGTAAGAACCTG+AGG	-	chr5.2:43293646-43293665	None:intergenic	40.0%
	CAACTCAACATTTGAGTCCT+CGG	-	chr5.2:43294167-43294186	None:intergenic	40.0%
	GATCATCGCAGCTTATTTGT+TGG	+	chr5.2:43293566-43293585	MS.gene042719:CDS	40.0%
	TTTGATATCATCAGCGGAAG+GGG	-	chr5.2:43293613-43293632	None:intergenic	40.0%
!	CTTCTTTTCCTCCTTTGCTT+CGG	-	chr5.2:43294332-43294351	None:intergenic	40.0%
!!	GTTTTGATATCATCAGCGGA+AGG	-	chr5.2:43293615-43293634	None:intergenic	40.0%
!!	TATTTGTTGGCTGTTTTGGG+CGG	+	chr5.2:43293579-43293598	MS.gene042719:CDS	40.0%
!!!	CATCTTTGTTTTGCAGGACA+TGG	+	chr5.2:43294454-43294473	MS.gene042719:intron	40.0%
!!!	GTTGCTTTTGAGTGAAGTCA+AGG	+	chr5.2:43294183-43294202	MS.gene042719:CDS	40.0%
	CGAAGCAAAGGAGGAAAAGA+AGG	+	chr5.2:43294330-43294349	MS.gene042719:CDS	45.0%
	TATAGTTGGAGTTGAAGCCG+AGG	+	chr5.2:43294147-43294166	MS.gene042719:intron	45.0%
	TCTGCCACTAGCAATTAGCT+CGG	-	chr5.2:43294221-43294240	None:intergenic	45.0%
	TTTGAGTGAAGTCAAGGGCA+AGG	+	chr5.2:43294189-43294208	MS.gene042719:CDS	45.0%
	TTTGCCGAGCTAATTGCTAG+TGG	+	chr5.2:43294214-43294233	MS.gene042719:CDS	45.0%
!	GCTTTAGCTCACTTCTTCAG+AGG	-	chr5.2:43294041-43294060	None:intergenic	45.0%
	ATCAAAACCATCCTCGCCTC+AGG	+	chr5.2:43293627-43293646	MS.gene042719:CDS	50.0%
	TGCTAGTGGCAGAGAGAAGT+TGG	+	chr5.2:43294228-43294247	MS.gene042719:CDS	50.0%
!	AAGTTGGCTTCAGTGCCATC+TGG	+	chr5.2:43294244-43294263	MS.gene042719:CDS	50.0%
!!	GGTGTCGATTTGCCTGGTAT+CGG	+	chr5.2:43293930-43293949	MS.gene042719:intron	50.0%
!!!	GTTTTCGGTGTCGATTTGCC+TGG	+	chr5.2:43293924-43293943	MS.gene042719:intron	50.0%
	AGAGGTAAGAACCTGAGGCG+AGG	-	chr5.2:43293641-43293660	None:intergenic	55.0%
	GTAAGAACCTGAGGCGAGGA+TGG	-	chr5.2:43293637-43293656	None:intergenic	55.0%
	TACGGACAGACACCGATACC+AGG	-	chr5.2:43293945-43293964	None:intergenic	55.0%
	TTGGCTTCAGTGCCATCTGG+TGG	+	chr5.2:43294247-43294266	MS.gene042719:CDS	55.0%
	ACCAGCTGCCGAAGCAAAGG+AGG	+	chr5.2:43294321-43294340	MS.gene042719:CDS	60.0%
	GCTTCAGTGCCATCTGGTGG+AGG	+	chr5.2:43294250-43294269	MS.gene042719:CDS	60.0%
	TCCTCCTTTGCTTCGGCAGC+TGG	-	chr5.2:43294325-43294344	None:intergenic	60.0%
	TGCACCAGCTGCCGAAGCAA+AGG	+	chr5.2:43294318-43294337	MS.gene042719:CDS	60.0%
!	GCTGTGGCTGTTGCTGCTTC+CGG	+	chr5.2:43294274-43294293	MS.gene042719:CDS	60.0%
	AGCAGCTGCAGCACCTCCAC+CGG	-	chr5.2:43294296-43294315	None:intergenic	65.0%
	GCCACAGCACCTCCACCAGA+TGG	-	chr5.2:43294262-43294281	None:intergenic	65.0%
!	GCTGTTGCTGCTTCCGGTGG+AGG	+	chr5.2:43294280-43294299	MS.gene042719:CDS	65.0%
!	GTGGCTGTTGCTGCTTCCGG+TGG	+	chr5.2:43294277-43294296	MS.gene042719:CDS	65.0%
!!	GCCATCTGGTGGAGGTGCTG+TGG	+	chr5.2:43294258-43294277	MS.gene042719:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	43293561	43294496	43293561	ID=MS.gene042719
chr5.2	mRNA	43293561	43294496	43293561	ID=MS.gene042719.t1;Parent=MS.gene042719
chr5.2	exon	43293561	43293648	43293561	ID=MS.gene042719.t1.exon1;Parent=MS.gene042719.t1
chr5.2	CDS	43293561	43293648	43293561	ID=cds.MS.gene042719.t1;Parent=MS.gene042719.t1
chr5.2	exon	43294152	43294378	43294152	ID=MS.gene042719.t1.exon2;Parent=MS.gene042719.t1
chr5.2	CDS	43294152	43294378	43294152	ID=cds.MS.gene042719.t1;Parent=MS.gene042719.t1
chr5.2	exon	43294470	43294496	43294470	ID=MS.gene042719.t1.exon3;Parent=MS.gene042719.t1
chr5.2	CDS	43294470	43294496	43294470	ID=cds.MS.gene042719.t1;Parent=MS.gene042719.t1

Gene Sequence

>MS.gene042719

ATGAAGATCATCGCAGCTTATTTGTTGGCTGTTTTGGGCGGTAACGCTGCCCCTTCCGCTGATGATATCAAAACCATCCTCGCCTCAGGTTCTTACCTCTCTTTTTTTGGTTATGTTTTCATTAATCTGTTCTGTTTCTTTGTCTGTGTTCAGTGTCTGTTTTTTTTTTTTTGTTTTTGTTTGTAAAATTGATAAGTATTGAAAATATGTTATGTAGTTGAATGAATTAGGTTTGATTGTAATTGAATATAGTGGCGCAAGATTTCCACTCTTTGCAATTTTTTGTTTTAAAATATAATTTAAAAATACAATTTAAGAGCATTTCTAGATGTCTGACACAACACATAATTTCTACATTGAATTGTTTTCGGTGTCGATTTGCCTGGTATCGGTGTCTGTCCGTAGATTTTGATTATTGAAGCCTGATAAACATTGAATTTTGATTTATCCTTGACAAATCAATTTTTGGTCACTGAGCCTCTGAAGAAGTGAGCTAAAGCAAAACAATTGACATTTGATGCAACTTAAAACAGAAATAATTGAATTTTGAATGTCTTGAATGAATTTGCTTTTTCTTTGTTATTTTTATAGTTGGAGTTGAAGCCGAGGACTCAAATGTTGAGTTGCTTTTGAGTGAAGTCAAGGGCAAGGATTTTGCCGAGCTAATTGCTAGTGGCAGAGAGAAGTTGGCTTCAGTGCCATCTGGTGGAGGTGCTGTGGCTGTTGCTGCTTCCGGTGGAGGTGCTGCAGCTGCTGCTGCACCAGCTGCCGAAGCAAAGGAGGAAAAGAAGGTTGAAGAAAAGGAAGAATCCGATGATGTAAGTTTCTTTGTTATTTATAGTTGTTATTTAGTGAATTCATGACTCTCTACTAACATATGACTGTATCATTGTCATCTTTGTTTTGCAGGACATGGGATTTAGCTTGTTCGACTAG

Protein sequence

>MS.gene042719.t1

MKIIAAYLLAVLGGNAAPSADDIKTILASVGVEAEDSNVELLLSEVKGKDFAELIASGREKLASVPSGGGAVAVAASGGGAAAAAAPAAEAKEEKKVEEKEESDDDMGFSLFD