AlfalfaGEDB | MS.gene82009

M. sativa cultivar XinJiangDaYe / MS.gene82009

KEGG: K02942
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006414 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044249 GO:0044260 GO:0071704 GO:0110165 GO:1901576
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82009.t1	XP_013444548.1	100	60	0	0	1	60	1	60	7.90E-24	119.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82009.t1	Q8LCW9	61.7	60	23	0	2	61	3	62	6.1e-17	88.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82009.t1	I3S0D9	100.0	60	0	0	1	60	1	60	5.7e-24	119.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049387	MS.gene82009	0.820791	5.69E-53	-1.69E-46
MS.gene050134	MS.gene82009	0.84088	6.66E-58	-1.69E-46
MS.gene050791	MS.gene82009	0.802856	4.60E-49	-1.69E-46
MS.gene051984	MS.gene82009	0.82794	1.19E-54	-1.69E-46
MS.gene052666	MS.gene82009	0.862453	5.01E-64	-1.69E-46
MS.gene053224	MS.gene82009	0.826777	2.25E-54	-1.69E-46
MS.gene057272	MS.gene82009	0.832366	9.85E-56	-1.69E-46
MS.gene057321	MS.gene82009	0.869683	2.58E-66	-1.69E-46
MS.gene057643	MS.gene82009	0.847899	8.63E-60	-1.69E-46
MS.gene059340	MS.gene82009	0.810483	1.13E-50	-1.69E-46
MS.gene060483	MS.gene82009	0.93494	1.85E-96	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene82009	MS.gene08521	PPI
MS.gene06833	MS.gene82009	PPI
MS.gene82009	MS.gene045633	PPI
MS.gene76615	MS.gene82009	PPI
MS.gene82009	MS.gene057237	PPI
MS.gene069339	MS.gene82009	PPI
MS.gene82009	MS.gene060483	PPI
MS.gene82009	MS.gene067331	PPI
MS.gene82009	MS.gene76615	PPI
MS.gene82009	MS.gene91826	PPI
MS.gene82009	MS.gene97876	PPI
MS.gene44900	MS.gene82009	PPI
MS.gene82009	MS.gene053952	PPI
MS.gene82009	MS.gene01425	PPI
MS.gene073078	MS.gene82009	PPI
MS.gene82009	MS.gene032942	PPI
MS.gene009874	MS.gene82009	PPI
MS.gene82009	MS.gene35379	PPI
MS.gene82009	MS.gene01952	PPI
MS.gene82009	MS.gene88792	PPI
MS.gene82009	MS.gene047578	PPI
MS.gene82009	MS.gene06617	PPI
MS.gene042719	MS.gene82009	PPI
MS.gene013506	MS.gene82009	PPI
MS.gene82009	MS.gene002442	PPI
MS.gene82009	MS.gene01091	PPI
MS.gene43071	MS.gene82009	PPI
MS.gene82009	MS.gene78764	PPI
MS.gene08521	MS.gene82009	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82009.t1	MTR_8g024350	98.148	108	2	0	1	108	1	108	1.26e-72	211
MS.gene82009.t1	MTR_3g092600	60.000	110	42	1	1	108	1	110	4.73e-29	101
MS.gene82009.t1	MTR_2g066750	60.909	110	41	1	1	108	1	110	4.73e-29	101
MS.gene82009.t1	MTR_7g118060	66.102	59	20	0	1	59	1	59	1.53e-23	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82009.t1	AT5G24510	57.391	115	38	4	1	108	1	111	1.06e-37	123
MS.gene82009.t1	AT1G01100	51.402	107	39	1	2	108	3	96	7.04e-32	108
MS.gene82009.t1	AT4G00810	53.153	111	48	1	2	108	3	113	2.94e-31	107
MS.gene82009.t1	AT4G00810	53.153	111	48	1	2	108	3	113	2.94e-31	107
MS.gene82009.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene82009.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene82009.t1	AT1G01100	53.636	110	48	1	2	108	3	112	6.42e-31	106
MS.gene82009.t1	AT5G47700	53.153	111	48	1	2	108	3	113	9.37e-31	105
MS.gene82009.t1	AT5G47700	53.153	111	48	1	2	108	3	113	9.37e-31	105

Find 28 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGGAGGCAGCAGCAGCAAC+TGG	0.216487	8.2:-71465150	None:intergenic
ACGTTAATGTTGAAGCTTAC+TGG	0.234944	8.2:+71465034	MS.gene82009:CDS
TGGTGCTCCGGTTTCTGTTT+CGG	0.321080	8.2:+71465125	MS.gene82009:CDS
GTCGTCGTCACTCTCATCCT+TGG	0.357062	8.2:-71465300	None:intergenic
ATCATCAAGGTTCTTCTTCT+CGG	0.364681	8.2:-71465079	None:intergenic
ACTGGGGCCGAAACAGAAAC+CGG	0.462684	8.2:-71465132	None:intergenic
TCGTCGTCACTCTCATCCTT+GGG	0.464875	8.2:-71465299	None:intergenic
TGCCGCTGGAGGTGGTGCTC+CGG	0.491299	8.2:+71465113	MS.gene82009:CDS
ATATTGAATGCCGCTGGAGG+TGG	0.496313	8.2:+71465105	MS.gene82009:CDS
GCAAGTTTAGCGAATAAACT+TGG	0.520693	8.2:-71465057	None:intergenic
TGCTCCTATCGAGGAGAAGA+AGG	0.520970	8.2:+71465182	MS.gene82009:CDS
TGGAGGCAGCAGCAGCAACT+GGG	0.544694	8.2:-71465149	None:intergenic
AGGAGCAGCAACTGCAGTGG+AGG	0.545209	8.2:-71465166	None:intergenic
GGATGAGAGTGACGACGACA+TGG	0.547177	8.2:+71465304	MS.gene82009:CDS
GTTTGCAGCTTTCAACAAAG+TGG	0.560297	8.2:-71465013	None:intergenic
GATTTGATATTGAATGCCGC+TGG	0.580711	8.2:+71465099	MS.gene82009:CDS
AACCGGAGCACCACCTCCAG+CGG	0.583475	8.2:-71465115	None:intergenic
CGGCGATGTTATCATCATGA+AGG	0.588233	8.2:-71464297	None:intergenic
CATTCAATATCAAATCATCA+AGG	0.592309	8.2:-71465092	None:intergenic
TGCAGTTGCTGCTCCTATCG+AGG	0.607768	8.2:+71465173	MS.gene82009:CDS
GGAGGCAGCAGCAGCAACTG+GGG	0.621755	8.2:-71465148	None:intergenic
CCTACCTTCTTCTCCTCGAT+AGG	0.622145	8.2:-71465186	None:intergenic
GATAGGAGCAGCAACTGCAG+TGG	0.632714	8.2:-71465169	None:intergenic
TGTAGTGTGAAAATGTCGAT+CGG	0.636502	8.2:+71464244	None:intergenic
ATTATCAAAGGATACAGTGA+CGG	0.653291	8.2:-71464317	None:intergenic
GATGAGAGTGACGACGACAT+GGG	0.653294	8.2:+71465305	MS.gene82009:CDS
GTACTTACAGGAAGAACCCA+AGG	0.654187	8.2:+71465283	MS.gene82009:intron
TTGATATTGAATGCCGCTGG+AGG	0.659375	8.2:+71465102	MS.gene82009:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAACCTAATATTTTTTTA+AGG	-	chr8.2:71464705-71464724	None:intergenic	10.0%
!!!	AAAAATATTAGGTTTTTTAA+AGG	+	chr8.2:71464708-71464727	MS.gene82009:intron	10.0%
!!!	AAAATCAATTTTTATATAGT+GGG	+	chr8.2:71464858-71464877	MS.gene82009:intron	10.0%
!!!	TAAAATCAATTTTTATATAG+TGG	+	chr8.2:71464857-71464876	MS.gene82009:intron	10.0%
!!	AAAAATTCAGTAATAATTTG+AGG	-	chr8.2:71464573-71464592	None:intergenic	15.0%
!!	ATTACATTATCAATTATCAA+AGG	-	chr8.2:71464332-71464351	None:intergenic	15.0%
!!	TTAGTCCTTAAAAAAATATT+AGG	+	chr8.2:71464697-71464716	MS.gene82009:intron	15.0%
!!!	AAAAATTGATTTTAGAACTT+TGG	-	chr8.2:71464851-71464870	None:intergenic	15.0%
!!!	ACTAAATTAACAATTTTCTA+CGG	+	chr8.2:71464818-71464837	MS.gene82009:intron	15.0%
!!!	ATTATTACTGAATTTTTTAC+TGG	+	chr8.2:71464576-71464595	MS.gene82009:intron	15.0%
!!!	TTGAAAAAAAGAGATTTTTT+TGG	+	chr8.2:71464424-71464443	MS.gene82009:intron	15.0%
!!	AAATTCAGAATATAATTCCT+AGG	+	chr8.2:71464634-71464653	MS.gene82009:intron	20.0%
!!!	TCTTATCTAATTGGTATTTT+TGG	+	chr8.2:71465258-71465277	MS.gene82009:intron	20.0%
!	CATTCAATATCAAATCATCA+AGG	-	chr8.2:71465095-71465114	None:intergenic	25.0%
!	TAATGAATTTAGAAGATGCT+AGG	+	chr8.2:71464392-71464411	MS.gene82009:intron	25.0%
!!	ATTAACAATTTTCTACGGTT+AGG	+	chr8.2:71464823-71464842	MS.gene82009:intron	25.0%
!!	TTAACAATTTTCTACGGTTA+GGG	+	chr8.2:71464824-71464843	MS.gene82009:intron	25.0%
!!!	AGATTTTTTTGGATGATACA+AGG	+	chr8.2:71464435-71464454	MS.gene82009:intron	25.0%
!!!	ATTTTTTTGGATGATACAAG+GGG	+	chr8.2:71464437-71464456	MS.gene82009:intron	25.0%
!!!	GAATTTTGTTTTGGTGAATA+AGG	+	chr8.2:71464893-71464912	MS.gene82009:intron	25.0%
!!!	GATTTTTTTGGATGATACAA+GGG	+	chr8.2:71464436-71464455	MS.gene82009:intron	25.0%
!!!	TTTTTATATAGTGGGAATCT+CGG	+	chr8.2:71464866-71464885	MS.gene82009:intron	25.0%
	AATGACAATTAACAACTCCT+AGG	-	chr8.2:71464654-71464673	None:intergenic	30.0%
	ATTATCAAAGGATACAGTGA+CGG	-	chr8.2:71464320-71464339	None:intergenic	30.0%
	ATTTAGAAGATGCTAGGAAT+TGG	+	chr8.2:71464398-71464417	MS.gene82009:intron	30.0%
	TAAAGTTCTTGGATAGAGTT+AGG	+	chr8.2:71464757-71464776	MS.gene82009:intron	30.0%
	TCCTTAAGTCATTGTTGATA+GGG	+	chr8.2:71464979-71464998	MS.gene82009:intron	30.0%
	TGTTGGTCATCTTATCTAAT+TGG	+	chr8.2:71465249-71465268	MS.gene82009:intron	30.0%
	TTTAGAAGATGCTAGGAATT+GGG	+	chr8.2:71464399-71464418	MS.gene82009:intron	30.0%
!!!	AGATAGCGTGTTTTAAAGAT+TGG	+	chr8.2:71464952-71464971	MS.gene82009:intron	30.0%
!!!	CTCGGTTTTGAATTTTGTTT+TGG	+	chr8.2:71464884-71464903	MS.gene82009:intron	30.0%
!!!	GTATTTTTGGTTGTACTTAC+AGG	+	chr8.2:71465271-71465290	MS.gene82009:intron	30.0%
	ACCCTATCAACAATGACTTA+AGG	-	chr8.2:71464983-71465002	None:intergenic	35.0%
	ACGTTAATGTTGAAGCTTAC+TGG	+	chr8.2:71465034-71465053	MS.gene82009:CDS	35.0%
	ATCATCAAGGTTCTTCTTCT+CGG	-	chr8.2:71465082-71465101	None:intergenic	35.0%
	CTCCTTAAGTCATTGTTGAT+AGG	+	chr8.2:71464978-71464997	MS.gene82009:intron	35.0%
	GAACTTTGTCTTCTGTTTGT+TGG	+	chr8.2:71465232-71465251	MS.gene82009:intron	35.0%
	GCAAGTTTAGCGAATAAACT+TGG	-	chr8.2:71465060-71465079	None:intergenic	35.0%
	TGAGTGCTAGTTAAAGTTCT+TGG	+	chr8.2:71464746-71464765	MS.gene82009:intron	35.0%
	TTTGGTGAATAAGGAAAGTG+TGG	+	chr8.2:71464902-71464921	MS.gene82009:intron	35.0%
!	AATAAGGAAAGTGTGGTGTA+AGG	+	chr8.2:71464909-71464928	MS.gene82009:intron	35.0%
!	ATAAGGAAAGTGTGGTGTAA+GGG	+	chr8.2:71464910-71464929	MS.gene82009:intron	35.0%
	GATGATACAAGGGGCTATAT+AGG	+	chr8.2:71464446-71464465	MS.gene82009:intron	40.0%
	GATTTGATATTGAATGCCGC+TGG	+	chr8.2:71465099-71465118	MS.gene82009:CDS	40.0%
	GTTTGCAGCTTTCAACAAAG+TGG	-	chr8.2:71465016-71465035	None:intergenic	40.0%
!	AGGAGAAGAAGGTAGGTTTT+TGG	+	chr8.2:71465193-71465212	MS.gene82009:intron	40.0%
!!!	TCGATCAGAAGTGTCGTTTT+AGG	-	chr8.2:71464480-71464499	None:intergenic	40.0%
	CGGCGATGTTATCATCATGA+AGG	-	chr8.2:71464300-71464319	None:intergenic	45.0%
	CTTCTGATCGAAGTTGTGTC+TGG	+	chr8.2:71464489-71464508	MS.gene82009:intron	45.0%
	GTACTTACAGGAAGAACCCA+AGG	+	chr8.2:71465283-71465302	MS.gene82009:intron	45.0%
	TTGATATTGAATGCCGCTGG+AGG	+	chr8.2:71465102-71465121	MS.gene82009:CDS	45.0%
!!!	TTTTTTTATTTTCTTATTTT+AGG	+	chr8.2:71464795-71464814	MS.gene82009:intron	5.0%
	ATATTGAATGCCGCTGGAGG+TGG	+	chr8.2:71465105-71465124	MS.gene82009:CDS	50.0%
	CCTACCTTCTTCTCCTCGAT+AGG	-	chr8.2:71465189-71465208	None:intergenic	50.0%
	CCTATCGAGGAGAAGAAGGT+AGG	+	chr8.2:71465186-71465205	MS.gene82009:intron	50.0%
	GATGAGAGTGACGACGACAT+GGG	+	chr8.2:71465305-71465324	MS.gene82009:CDS	50.0%
	TCGTCGTCACTCTCATCCTT+GGG	-	chr8.2:71465302-71465321	None:intergenic	50.0%
	TGCTCCTATCGAGGAGAAGA+AGG	+	chr8.2:71465182-71465201	MS.gene82009:CDS	50.0%
	TGGTGCTCCGGTTTCTGTTT+CGG	+	chr8.2:71465125-71465144	MS.gene82009:CDS	50.0%
	TGGTGTGTGTCAGTGCTTCA+TGG	+	chr8.2:71464596-71464615	MS.gene82009:intron	50.0%
	ACTGGGGCCGAAACAGAAAC+CGG	-	chr8.2:71465135-71465154	None:intergenic	55.0%
	GATAGGAGCAGCAACTGCAG+TGG	-	chr8.2:71465172-71465191	None:intergenic	55.0%
	GGATGAGAGTGACGACGACA+TGG	+	chr8.2:71465304-71465323	MS.gene82009:CDS	55.0%
	GTCGTCGTCACTCTCATCCT+TGG	-	chr8.2:71465303-71465322	None:intergenic	55.0%
	TGCAGTTGCTGCTCCTATCG+AGG	+	chr8.2:71465173-71465192	MS.gene82009:CDS	55.0%
	AGGAGCAGCAACTGCAGTGG+AGG	-	chr8.2:71465169-71465188	None:intergenic	60.0%
	TGGAGGCAGCAGCAGCAACT+GGG	-	chr8.2:71465152-71465171	None:intergenic	60.0%
	GGAGGCAGCAGCAGCAACTG+GGG	-	chr8.2:71465151-71465170	None:intergenic	65.0%
	GTGGAGGCAGCAGCAGCAAC+TGG	-	chr8.2:71465153-71465172	None:intergenic	65.0%
!	AACCGGAGCACCACCTCCAG+CGG	-	chr8.2:71465118-71465137	None:intergenic	65.0%
	TGCCGCTGGAGGTGGTGCTC+CGG	+	chr8.2:71465113-71465132	MS.gene82009:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	71464256	71465346	71464256	ID=MS.gene82009
chr8.2	mRNA	71464256	71465346	71464256	ID=MS.gene82009.t1;Parent=MS.gene82009
chr8.2	exon	71464256	71464324	71464256	ID=MS.gene82009.t1.exon1;Parent=MS.gene82009.t1
chr8.2	CDS	71464256	71464324	71464256	ID=cds.MS.gene82009.t1;Parent=MS.gene82009.t1
chr8.2	exon	71465000	71465203	71465000	ID=MS.gene82009.t1.exon2;Parent=MS.gene82009.t1
chr8.2	CDS	71465000	71465203	71465000	ID=cds.MS.gene82009.t1;Parent=MS.gene82009.t1
chr8.2	exon	71465293	71465346	71465293	ID=MS.gene82009.t1.exon3;Parent=MS.gene82009.t1
chr8.2	CDS	71465293	71465346	71465293	ID=cds.MS.gene82009.t1;Parent=MS.gene82009.t1

Gene Sequence

>MS.gene82009

ATGTCGATCGGAGAAGTTGCTTGCAGCTACTCACTTATGATCCTTCATGATGATAACATCGCCGTCACTGTATCCTTTGATAATTGATAATGTAATTAATTTAACTATGATTTTCTTTTGTTAGTATATGTTTTTTTAATGAATTTAGAAGATGCTAGGAATTGGGTTTTGAAAAAAAGAGATTTTTTTGGATGATACAAGGGGCTATATAGGATTATGAACCTAAAACGACACTTCTGATCGAAGTTGTGTCTGGTGTCTTTTGCGTGTTTTGTCAGCGCACTCACATTCAATTACATTTGATTAATTCATTTCCTCAAATTATTACTGAATTTTTTACTGGTGTGTGTCAGTGCTTCATGGTCTCTGATGCAAGTGAAATTCAGAATATAATTCCTAGGAGTTGTTAATTGTCATTTAGATTCAATTATTGAAATCAAATTAGTCCTTAAAAAAATATTAGGTTTTTTAAAGGTGAAACTAGTTTTGATGAGTGCTAGTTAAAGTTCTTGGATAGAGTTAGGATAATGTTTTTGTGATTTTTTTATTTTCTTATTTTAGGACTAAATTAACAATTTTCTACGGTTAGGGACCAAAGTTCTAAAATCAATTTTTATATAGTGGGAATCTCGGTTTTGAATTTTGTTTTGGTGAATAAGGAAAGTGTGGTGTAAGGGTTTGAAGTTGAATATTTGAAGATAGCGTGTTTTAAAGATTGGTTTCTCCTTAAGTCATTGTTGATAGGGTGAGAAAATCTCCACTTTGTTGAAAGCTGCAAACGTTAATGTTGAAGCTTACTGGCCAAGTTTATTCGCTAAACTTGCCGAGAAGAAGAACCTTGATGATTTGATATTGAATGCCGCTGGAGGTGGTGCTCCGGTTTCTGTTTCGGCCCCAGTTGCTGCTGCTGCCTCCACTGCAGTTGCTGCTCCTATCGAGGAGAAGAAGGTAGGTTTTTGGTGAAAAAGTTGTTGTTGAACTTTGTCTTCTGTTTGTTGGTCATCTTATCTAATTGGTATTTTTGGTTGTACTTACAGGAAGAACCCAAGGATGAGAGTGACGACGACATGGGATTCAGCTTGTTTGATTAG

Protein sequence

>MS.gene82009.t1

MSIGEVACSYSLMILHDDNIAVTGEKISTLLKAANVNVEAYWPSLFAKLAEKKNLDDLILNAAGGGAPVSVSAPVAAAASTAVAAPIEEKKEEPKDESDDDMGFSLFD