AlfalfaGEDB | MS.gene045633

M. sativa cultivar XinJiangDaYe / MS.gene045633

KEGG: K02942
GO: -
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045633.t1	XP_013464220.1	87.7	65	8	0	1	65	1	65	1.50E-22	115.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045633.t1	P52855	60.3	63	25	0	1	63	1	63	5.9e-15	82.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045633.t1	A0A072VJD3	87.7	65	8	0	1	65	1	65	1.1e-22	115.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene045633	MS.gene97876	PPI
MS.gene045633	MS.gene053952	PPI
MS.gene44900	MS.gene045633	PPI
MS.gene045633	MS.gene01425	PPI
MS.gene82009	MS.gene045633	PPI
MS.gene073078	MS.gene045633	PPI
MS.gene045633	MS.gene032942	PPI
MS.gene009874	MS.gene045633	PPI
MS.gene045633	MS.gene35379	PPI
MS.gene045633	MS.gene01952	PPI
MS.gene002442	MS.gene045633	PPI
MS.gene045633	MS.gene047578	PPI
MS.gene060483	MS.gene045633	PPI
MS.gene06617	MS.gene045633	PPI
MS.gene08521	MS.gene045633	PPI
MS.gene045633	MS.gene06617	PPI
MS.gene042719	MS.gene045633	PPI
MS.gene013506	MS.gene045633	PPI
MS.gene045633	MS.gene88792	PPI
MS.gene76615	MS.gene045633	PPI
MS.gene057237	MS.gene045633	PPI
MS.gene88792	MS.gene045633	PPI
MS.gene045633	MS.gene01091	PPI
MS.gene01952	MS.gene045633	PPI
MS.gene43071	MS.gene045633	PPI
MS.gene06833	MS.gene045633	PPI
MS.gene067331	MS.gene045633	PPI
MS.gene045633	MS.gene057237	PPI
MS.gene069339	MS.gene045633	PPI
MS.gene045633	MS.gene91826	PPI
MS.gene045633	MS.gene78764	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045633.t1	MTR_3g092600	86.885	61	8	0	1	61	1	61	2.20e-31	108
MS.gene045633.t1	MTR_2g066750	86.885	61	8	0	1	61	1	61	2.20e-31	108
MS.gene045633.t1	MTR_8g024350	63.014	73	26	1	1	73	1	72	2.90e-24	90.5
MS.gene045633.t1	MTR_7g118060	59.322	59	24	0	1	59	1	59	7.94e-18	74.3
MS.gene045633.t1	MTR_7g005740	56.452	62	8	1	1	62	1	43	1.32e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045633.t1	AT5G24510	50.000	94	46	1	1	94	1	93	3.93e-24	90.1
MS.gene045633.t1	AT5G47700	56.667	60	26	0	2	61	3	62	3.87e-18	75.1
MS.gene045633.t1	AT5G47700	56.667	60	26	0	2	61	3	62	3.87e-18	75.1
MS.gene045633.t1	AT1G01100	56.667	60	26	0	2	61	3	62	4.81e-18	74.3
MS.gene045633.t1	AT1G01100	56.667	60	26	0	2	61	3	62	7.68e-18	74.3
MS.gene045633.t1	AT1G01100	56.667	60	26	0	2	61	3	62	7.68e-18	74.3
MS.gene045633.t1	AT1G01100	56.667	60	26	0	2	61	3	62	7.68e-18	74.3
MS.gene045633.t1	AT4G00810	55.000	60	27	0	2	61	3	62	2.01e-17	73.2
MS.gene045633.t1	AT4G00810	55.000	60	27	0	2	61	3	62	2.01e-17	73.2

Find 39 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGACTAGGACTCCTTTCC+AGG	0.246566	5.3:-29253597	MS.gene045633:CDS
CTCATCGTCACTTTCTTCCT+TGG	0.284631	5.3:+29253668	None:intergenic
AGATAGTAGATTGCAAGTTT+TGG	0.304388	5.3:+29253562	None:intergenic
ACGTCATCGTTGAATCTTAC+TGG	0.332894	5.3:-29253850	MS.gene045633:CDS
CAAAATGAGTTTCGGCGAAT+CGG	0.351766	5.3:-29254051	None:intergenic
AAAATGAGTTTCGGCGAATC+GGG	0.370184	5.3:-29254050	MS.gene045633:CDS
GCCGTATCTGCACCTGCCTC+TGG	0.406228	5.3:-29253743	MS.gene045633:CDS
ACAATTCACAAAATGAGTTT+CGG	0.409091	5.3:-29254059	None:intergenic
CCTGGAAAGGAGTCCTAGTC+TGG	0.424028	5.3:+29253597	None:intergenic
TATGAGCAAGCTTAGCGAAT+AGG	0.439056	5.3:+29253822	None:intergenic
CGGCAGTTCGGCTGCTGTTG+CGG	0.464200	5.3:+29253712	None:intergenic
GATAGCAGACTTACGGGGAT+AGG	0.472811	5.3:+29253987	None:intergenic
ATCGTTGAATCTTACTGGCC+AGG	0.475602	5.3:-29253845	MS.gene045633:CDS
TCTGCACCTGCCTCTGGAGG+TGG	0.475703	5.3:-29253737	MS.gene045633:CDS
GCGGCTCCACCTCCAGAGGC+AGG	0.482646	5.3:+29253731	None:intergenic
GAAGATCGGTACTTTGTTGA+AGG	0.484661	5.3:-29253879	MS.gene045633:CDS
GGAAGAAAGTGACGATGAGA+TGG	0.490298	5.3:-29253664	MS.gene045633:CDS
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	5.3:-29253776	MS.gene045633:CDS
TCCAGAGGCAGGTGCAGATA+CGG	0.511601	5.3:+29253742	None:intergenic
ATAGCAGACTTACGGGGATA+GGG	0.518236	5.3:+29253988	None:intergenic
TAGTTTGTTGTAGCCAGACT+AGG	0.521961	5.3:-29253610	MS.gene045633:CDS
TTTGTTTAGGCGAAGAAGAT+CGG	0.532771	5.3:-29253893	MS.gene045633:intron
TCCATCGTCGTGGAGGATCA+AGG	0.532980	5.3:+29254013	None:intergenic
GCAAGCTTAGCGAATAGGCC+TGG	0.533765	5.3:+29253827	None:intergenic
AGATTCAACGATGACGTTAG+CGG	0.535885	5.3:+29253856	None:intergenic
TGAAAAGAAAGAGGAAGCCA+AGG	0.547427	5.3:-29253685	MS.gene045633:CDS
TGCGGCACCACCAACAGCGC+CGG	0.548111	5.3:+29253769	None:intergenic
TGCCGCTGTTGAAAAGAAAG+AGG	0.552889	5.3:-29253694	MS.gene045633:CDS
ACCTTGATCCTCCACGACGA+TGG	0.573503	5.3:-29254014	MS.gene045633:CDS
CAGACTAGGACTCCTTTCCA+GGG	0.586881	5.3:-29253596	MS.gene045633:CDS
GAAGAAAGTGACGATGAGAT+GGG	0.590119	5.3:-29253663	MS.gene045633:CDS
TGTTGCGGCTCCACCTCCAG+AGG	0.619698	5.3:+29253727	None:intergenic
GTATCTGCACCTGCCTCTGG+AGG	0.631929	5.3:-29253740	MS.gene045633:CDS
GACTAGGACTCCTTTCCAGG+GGG	0.634640	5.3:-29253594	MS.gene045633:CDS
AGGTGCAGATACGGCAACTG+CGG	0.650374	5.3:+29253751	None:intergenic
TAGGGATTCCATCGTCGTGG+AGG	0.656879	5.3:+29254006	None:intergenic
AGACTAGGACTCCTTTCCAG+GGG	0.677366	5.3:-29253595	MS.gene045633:CDS
GGATAGGGATTCCATCGTCG+TGG	0.687033	5.3:+29254003	None:intergenic
ATCGTCGTGGAGGATCAAGG+TGG	0.738070	5.3:+29254016	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTCTTTGTTACTGTTT+GGG	-	chr5.3:29253670-29253689	MS.gene045633:CDS	20.0%
!	TACAGAAATCGAATTGAATA+GGG	+	chr5.3:29253626-29253645	None:intergenic	25.0%
!	TTACAGAAATCGAATTGAAT+AGG	+	chr5.3:29253627-29253646	None:intergenic	25.0%
!!	CATTTTTCTTTGTTACTGTT+TGG	-	chr5.3:29253669-29253688	MS.gene045633:CDS	25.0%
	AATGAAATCTATGAGCGATT+TGG	+	chr5.3:29253653-29253672	None:intergenic	30.0%
!	AGATAGTAGATTGCAAGTTT+TGG	+	chr5.3:29254029-29254048	None:intergenic	30.0%
!!	TACTGTTTGGGTTTTTGTTT+AGG	-	chr5.3:29253682-29253701	MS.gene045633:CDS	30.0%
	TTTGTTTAGGCGAAGAAGAT+CGG	-	chr5.3:29253695-29253714	MS.gene045633:CDS	35.0%
!	TTCCTCTTTCTTTTCAACAG+CGG	+	chr5.3:29253899-29253918	None:intergenic	35.0%
	AATAGGGATAGCAGACTTAC+GGG	+	chr5.3:29253610-29253629	None:intergenic	40.0%
	ACGTCATCGTTGAATCTTAC+TGG	-	chr5.3:29253738-29253757	MS.gene045633:CDS	40.0%
	AGATTCAACGATGACGTTAG+CGG	+	chr5.3:29253735-29253754	None:intergenic	40.0%
	GAAGAAAGTGACGATGAGAT+GGG	-	chr5.3:29253925-29253944	MS.gene045633:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr5.3:29253709-29253728	MS.gene045633:CDS	40.0%
	GAATAGGGATAGCAGACTTA+CGG	+	chr5.3:29253611-29253630	None:intergenic	40.0%
	TATGAGCAAGCTTAGCGAAT+AGG	+	chr5.3:29253769-29253788	None:intergenic	40.0%
	TGAAAAGAAAGAGGAAGCCA+AGG	-	chr5.3:29253903-29253922	MS.gene045633:intron	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr5.3:29253800-29253819	MS.gene045633:CDS	40.0%
!!	TAGTTTGTTGTAGCCAGACT+AGG	-	chr5.3:29253978-29253997	MS.gene045633:intron	40.0%
	ATAGCAGACTTACGGGGATA+GGG	+	chr5.3:29253603-29253622	None:intergenic	45.0%
	ATAGGGATAGCAGACTTACG+GGG	+	chr5.3:29253609-29253628	None:intergenic	45.0%
	ATCGTTGAATCTTACTGGCC+AGG	-	chr5.3:29253743-29253762	MS.gene045633:CDS	45.0%
	GGAAGAAAGTGACGATGAGA+TGG	-	chr5.3:29253924-29253943	MS.gene045633:intron	45.0%
	TGCCGCTGTTGAAAAGAAAG+AGG	-	chr5.3:29253894-29253913	MS.gene045633:intron	45.0%
!	CTCATCGTCACTTTCTTCCT+TGG	+	chr5.3:29253923-29253942	None:intergenic	45.0%
!	TCTTTTCAACAGCGGCAGTT+CGG	+	chr5.3:29253891-29253910	None:intergenic	45.0%
	AGACTAGGACTCCTTTCCAG+GGG	-	chr5.3:29253993-29254012	MS.gene045633:intron	50.0%
	CAGACTAGGACTCCTTTCCA+GGG	-	chr5.3:29253992-29254011	MS.gene045633:intron	50.0%
	GATAGCAGACTTACGGGGAT+AGG	+	chr5.3:29253604-29253623	None:intergenic	50.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr5.3:29253574-29253593	MS.gene045633:CDS	55.0%
	AGGTGCAGATACGGCAACTG+CGG	+	chr5.3:29253840-29253859	None:intergenic	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr5.3:29253575-29253594	None:intergenic	55.0%
	CCAGACTAGGACTCCTTTCC+AGG	-	chr5.3:29253991-29254010	MS.gene045633:intron	55.0%
	CCTGGAAAGGAGTCCTAGTC+TGG	+	chr5.3:29253994-29254013	None:intergenic	55.0%
	GACTAGGACTCCTTTCCAGG+GGG	-	chr5.3:29253994-29254013	MS.gene045633:intron	55.0%
	GCAAGCTTAGCGAATAGGCC+TGG	+	chr5.3:29253764-29253783	None:intergenic	55.0%
	GGATAGGGATTCCATCGTCG+TGG	+	chr5.3:29253588-29253607	None:intergenic	55.0%
	TAGGGATTCCATCGTCGTGG+AGG	+	chr5.3:29253585-29253604	None:intergenic	55.0%
	TCCAGAGGCAGGTGCAGATA+CGG	+	chr5.3:29253849-29253868	None:intergenic	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr5.3:29253578-29253597	None:intergenic	55.0%
!	TTTTGGATTCAGCCACCCCC+TGG	+	chr5.3:29254012-29254031	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr5.3:29253809-29253828	MS.gene045633:CDS	55.0%
	GATTCAGCCACCCCCTGGAA+AGG	+	chr5.3:29254007-29254026	None:intergenic	60.0%
	GTATCTGCACCTGCCTCTGG+AGG	-	chr5.3:29253848-29253867	MS.gene045633:CDS	60.0%
	TAGGACTCCTTTCCAGGGGG+TGG	-	chr5.3:29253997-29254016	MS.gene045633:intron	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr5.3:29253812-29253831	MS.gene045633:CDS	60.0%
	CGGCAGTTCGGCTGCTGTTG+CGG	+	chr5.3:29253879-29253898	None:intergenic	65.0%
	GCCGTATCTGCACCTGCCTC+TGG	-	chr5.3:29253845-29253864	MS.gene045633:CDS	65.0%
	TGTTGCGGCTCCACCTCCAG+AGG	+	chr5.3:29253864-29253883	None:intergenic	65.0%
!	TCTGCACCTGCCTCTGGAGG+TGG	-	chr5.3:29253851-29253870	MS.gene045633:CDS	65.0%
	TGCGGCACCACCAACAGCGC+CGG	+	chr5.3:29253822-29253841	None:intergenic	70.0%
	GCGGCTCCACCTCCAGAGGC+AGG	+	chr5.3:29253860-29253879	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	29253541	29254069	29253541	ID=MS.gene045633
chr5.3	mRNA	29253541	29254069	29253541	ID=MS.gene045633.t1;Parent=MS.gene045633
chr5.3	exon	29254001	29254069	29254001	ID=MS.gene045633.t1.exon1;Parent=MS.gene045633.t1
chr5.3	CDS	29254001	29254069	29254001	ID=cds.MS.gene045633.t1;Parent=MS.gene045633.t1
chr5.3	exon	29253541	29253906	29253541	ID=MS.gene045633.t1.exon2;Parent=MS.gene045633.t1
chr5.3	CDS	29253541	29253906	29253541	ID=cds.MS.gene045633.t1;Parent=MS.gene045633.t1

Gene Sequence

>MS.gene045633

ATGAGTTTCGGCGAATCGGGCTGCATTTGCGCCACCTTGATCCTCCACGACGATGGAATCCCTATCCCCGTAAGTCTGCTATCCCTATTCAATTCGATTTCTGTAATCTCCAAATCGCTCATAGATTTCATTTTTCTTTGTTACTGTTTGGGTTTTTGTTTAGGCGAAGAAGATCGGTACTTTGTTGAAGGCCGCTAACGTCATCGTTGAATCTTACTGGCCAGGCCTATTCGCTAAGCTTGCTCATAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCGCAGTTGCCGTATCTGCACCTGCCTCTGGAGGTGGAGCCGCAACAGCAGCCGAACTGCCGCTGTTGAAAAGAAAGAGGAAGCCAAGGAAGAAAGTGACGATGAGATGGGCTTTAGTTTGTTTGATTAAGTTTCTCTGTATAGTTTGTTGTAGCCAGACTAGGACTCCTTTCCAGGGGGTGGCTGAATCCAAAACTTGCAATCTACTATCTCTTTATAAATTGTTTATCTAA

Protein sequence

>MS.gene045633.t1

MSFGESGCICATLILHDDGIPIPAKKIGTLLKAANVIVESYWPGLFAKLAHSKNVDDLVLNSGAVGGAAVAVSAPASGGGAATAAELPLLKRKRKPRKKVTMRWALVCLIKFLCIVCCSQTRTPFQGVAESKTCNLLSLYKLFI