AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43071


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43071.t1 MTR_2g085085 96.460 113 4 0 1 113 1 113 4.48e-70 205
MS.gene43071.t1 MTR_0227s0020 87.611 113 14 0 1 113 1 113 6.53e-38 124
MS.gene43071.t1 MTR_1g060600 73.451 113 29 1 1 113 1 112 1.06e-29 103
MS.gene43071.t1 MTR_2g085105 96.552 29 1 0 1 29 1 29 1.01e-12 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43071.t1 AT2G27710 77.143 70 16 0 1 70 1 70 9.01e-32 108
MS.gene43071.t1 AT3G44590 67.257 113 35 2 1 113 1 111 8.06e-30 103
MS.gene43071.t1 AT3G44590 67.257 113 35 2 1 113 1 111 8.06e-30 103
MS.gene43071.t1 AT2G27720 66.087 115 37 1 1 113 1 115 1.86e-27 97.8
MS.gene43071.t1 AT2G27710 75.385 65 16 0 1 65 1 65 2.36e-25 92.4
MS.gene43071.t1 AT2G27710 75.385 65 16 0 1 65 1 65 2.36e-25 92.4
MS.gene43071.t1 AT2G27710 75.385 65 16 0 1 65 1 65 2.36e-25 92.4
MS.gene43071.t1 AT3G28500 55.652 115 49 2 1 113 1 115 3.03e-25 92.0
MS.gene43071.t1 AT2G27720 59.843 127 37 2 1 113 1 127 6.44e-24 89.0
MS.gene43071.t1 AT2G27720 57.692 130 38 2 1 113 1 130 1.39e-22 85.9
MS.gene43071.t1 AT5G40040 57.895 114 47 1 1 113 1 114 2.22e-16 69.3
MS.gene43071.t1 AT2G27720 66.667 87 27 1 29 113 5 91 5.49e-13 60.1

Find 34 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTACTTGTTGGCCGTTT+TGG 0.128749 2.4:+14184710 MS.gene43071:CDS
GCTGATCTTAAACAAATTCT+TGG 0.267351 2.4:+14184756 MS.gene43071:CDS
GTTGCTGTGTCTGCTGCAAC+TGG 0.362343 2.4:+14185960 MS.gene43071:CDS
TCTTCCTTTGCTGCTTCAGC+AGG 0.364467 2.4:-14186005 None:intergenic
CTTAAACAAATTCTTGGATC+AGG 0.372982 2.4:+14184762 MS.gene43071:CDS
GTTATCTTTGTGCAGGATAT+GGG 0.399106 2.4:+14186134 MS.gene43071:intron
AGAGAAGAAGGTGGAAGAGA+AGG 0.434796 2.4:+14186025 MS.gene43071:CDS
GATTATCGCAGCTTACTTGT+TGG 0.445399 2.4:+14184701 MS.gene43071:CDS
GTGTCTGCTGCAACTGGAGG+AGG 0.445905 2.4:+14185966 MS.gene43071:CDS
TGCGCCTGCTGAAGCAGCAA+AGG 0.454235 2.4:+14186001 MS.gene43071:CDS
GATCAGCAGCTGAAGGGGAT+TGG 0.469713 2.4:-14184740 None:intergenic
CCTTGCCCTTGACTTCACTT+AGG 0.488662 2.4:-14185872 None:intergenic
TGCCAGTGGAAGAGAAAAGT+TGG 0.491656 2.4:+14185911 MS.gene43071:CDS
CACAGCAACAGCACCACCAC+CGG 0.500128 2.4:-14185946 None:intergenic
AGCAGCAAAGGAAGAGAAGA+AGG 0.518126 2.4:+14186013 MS.gene43071:CDS
GCTTCAGTTCCATCCGGTGG+TGG 0.522209 2.4:+14185933 MS.gene43071:CDS
GGATTGGTTGCCTCCCAAAA+CGG 0.534645 2.4:-14184724 None:intergenic
CAACTCAATGTTGCCATCCT+CGG 0.536919 2.4:-14185847 None:intergenic
GCAACAGCACCACCACCGGA+TGG 0.539099 2.4:-14185942 None:intergenic
TGTTTAAGATCAGCAGCTGA+AGG 0.541739 2.4:-14184747 None:intergenic
TCTTCCACTGGCAATAAGCT+CGG 0.547245 2.4:-14185901 None:intergenic
GTTTAAGATCAGCAGCTGAA+GGG 0.564265 2.4:-14184746 None:intergenic
TTTAAGATCAGCAGCTGAAG+GGG 0.564880 2.4:-14184745 None:intergenic
AAGTTGGCTTCAGTTCCATC+CGG 0.567453 2.4:+14185927 MS.gene43071:CDS
GTTGGAGTTGAGGCCGAGGA+TGG 0.573673 2.4:+14185834 MS.gene43071:intron
GTTGCTCCTAAGTGAAGTCA+AGG 0.590863 2.4:+14185866 MS.gene43071:CDS
GCTGTGTCTGCTGCAACTGG+AGG 0.592795 2.4:+14185963 MS.gene43071:CDS
TATGATTGTAGTTGGAGTTG+AGG 0.599920 2.4:+14185824 MS.gene43071:intron
TTTGCCGAGCTTATTGCCAG+TGG 0.606365 2.4:+14185897 MS.gene43071:CDS
TTGGCTTCAGTTCCATCCGG+TGG 0.607108 2.4:+14185930 MS.gene43071:CDS
CCTAAGTGAAGTCAAGGGCA+AGG 0.616488 2.4:+14185872 MS.gene43071:CDS
TGTAGTTGGAGTTGAGGCCG+AGG 0.617745 2.4:+14185830 MS.gene43071:intron
TTGCTCCTAAGTGAAGTCAA+GGG 0.680870 2.4:+14185867 MS.gene43071:CDS
AGCAAAGGAAGAGAAGAAGG+TGG 0.683753 2.4:+14186016 MS.gene43071:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGATTAAAGATTAAAT+TGG - chr2.4:14184800-14184819 None:intergenic 10.0%
!! ATAAGATTAAAGATTAAATT+GGG - chr2.4:14184799-14184818 None:intergenic 10.0%
!!! AAATAATTTTAATTCAAGTT+TGG + chr2.4:14185193-14185212 MS.gene43071:intron 10.0%
!!! AATAATTTTAATTCAAGTTT+GGG + chr2.4:14185194-14185213 MS.gene43071:intron 10.0%
!! ATGAAAATTGATCAAAATAT+TGG + chr2.4:14185275-14185294 MS.gene43071:intron 15.0%
!! ATTAAAATCAAACCAATATA+TGG - chr2.4:14185550-14185569 None:intergenic 15.0%
!! TTAAAATCAAACCAATATAT+GGG - chr2.4:14185549-14185568 None:intergenic 15.0%
!!! AAAAAAAGTGACTTTATTTT+TGG + chr2.4:14185798-14185817 MS.gene43071:intron 15.0%
!!! AAAAAAGTGACTTTATTTTT+GGG + chr2.4:14185799-14185818 MS.gene43071:intron 15.0%
!!! TTTAACTTTAATTCAAGTTT+GGG + chr2.4:14185227-14185246 MS.gene43071:intron 15.0%
!! AATAGTTTATGCAAATTCAT+AGG + chr2.4:14185463-14185482 MS.gene43071:intron 20.0%
!! ATGGATCTACTAAAATAATT+CGG - chr2.4:14184895-14184914 None:intergenic 20.0%
!! TAAAATCAAACCAATATATG+GGG - chr2.4:14185548-14185567 None:intergenic 20.0%
!!! AAATATTGGTTTATTTGGAT+TGG + chr2.4:14185289-14185308 MS.gene43071:intron 20.0%
!!! AATTTTGAAACTTACATCAT+CGG - chr2.4:14186057-14186076 None:intergenic 20.0%
!!! ATATTCTACACTTTTACTTA+TGG - chr2.4:14184914-14184933 None:intergenic 20.0%
!!! GATCAAAATATTGGTTTATT+TGG + chr2.4:14185284-14185303 MS.gene43071:intron 20.0%
!!! GGATTTGTTTAATTGATAAT+CGG + chr2.4:14185062-14185081 MS.gene43071:intron 20.0%
!!! GTATATTTTTTTACTGTTGA+AGG + chr2.4:14185685-14185704 MS.gene43071:intron 20.0%
!!! GTTTAACTTTAATTCAAGTT+TGG + chr2.4:14185226-14185245 MS.gene43071:intron 20.0%
!!! TGCTAATTTAATTCAAGTTT+AGG + chr2.4:14185041-14185060 MS.gene43071:intron 20.0%
!!! TTCATTTTTACAGATGTATT+AGG + chr2.4:14185609-14185628 MS.gene43071:intron 20.0%
! TAAAATCGATGAATGAACAT+GGG + chr2.4:14185091-14185110 MS.gene43071:intron 25.0%
! TTAAAATCGATGAATGAACA+TGG + chr2.4:14185090-14185109 MS.gene43071:intron 25.0%
!! TAGTAGTATTGTTTGTTACT+TGG + chr2.4:14186079-14186098 MS.gene43071:intron 25.0%
!! TTTTACTGTTGAAGGAAAAA+TGG + chr2.4:14185693-14185712 MS.gene43071:intron 25.0%
!!! ATTTTAATTCAAGTTTGGGT+TGG + chr2.4:14185198-14185217 MS.gene43071:intron 25.0%
!!! GCTTAGTTTTATACATTGTT+TGG + chr2.4:14185570-14185589 MS.gene43071:intron 25.0%
!!! TTTTAATTCAAGTTTGGGTT+GGG + chr2.4:14185199-14185218 MS.gene43071:intron 25.0%
AAATAATCAATCCTAGACTG+TGG + chr2.4:14184996-14185015 MS.gene43071:intron 30.0%
AATAATCAATCCTAGACTGT+GGG + chr2.4:14184997-14185016 MS.gene43071:intron 30.0%
CTTAAACAAATTCTTGGATC+AGG + chr2.4:14184762-14184781 MS.gene43071:CDS 30.0%
GCTGATCTTAAACAAATTCT+TGG + chr2.4:14184756-14184775 MS.gene43071:CDS 30.0%
TATCTACTGTTAGACTGTTA+AGG + chr2.4:14185325-14185344 MS.gene43071:intron 30.0%
!!! GCATTTTTTGTTCGTTTTTC+CGG + chr2.4:14184853-14184872 MS.gene43071:intron 30.0%
AATCAAACAGCCCCATATAT+TGG + chr2.4:14185535-14185554 MS.gene43071:intron 35.0%
GTTATCTTTGTGCAGGATAT+GGG + chr2.4:14186134-14186153 MS.gene43071:intron 35.0%
TATGATTGTAGTTGGAGTTG+AGG + chr2.4:14185824-14185843 MS.gene43071:intron 35.0%
TCGTTTGAGCTTAACTGTTT+GGG + chr2.4:14185130-14185149 MS.gene43071:intron 35.0%
TGTTATCTTTGTGCAGGATA+TGG + chr2.4:14186133-14186152 MS.gene43071:intron 35.0%
TTTGGGTCTATGATTGTAGT+TGG + chr2.4:14185816-14185835 MS.gene43071:intron 35.0%
CGGATACTAATCATCGAAAC+CGG - chr2.4:14184875-14184894 None:intergenic 40.0%
CTCGTTTGAGCTTAACTGTT+TGG + chr2.4:14185129-14185148 MS.gene43071:intron 40.0%
CTTCATTATACCCACAGTCT+AGG - chr2.4:14185010-14185029 None:intergenic 40.0%
GATTATCGCAGCTTACTTGT+TGG + chr2.4:14184701-14184720 MS.gene43071:CDS 40.0%
GCGTAATGTTATCTTTGTGC+AGG + chr2.4:14186127-14186146 MS.gene43071:intron 40.0%
TGAATGAACATGGGTCTGTT+TGG + chr2.4:14185100-14185119 MS.gene43071:intron 40.0%
TTGCTCCTAAGTGAAGTCAA+GGG + chr2.4:14185867-14185886 MS.gene43071:CDS 40.0%
! GTTTAAGATCAGCAGCTGAA+GGG - chr2.4:14184749-14184768 None:intergenic 40.0%
! TGTTTAAGATCAGCAGCTGA+AGG - chr2.4:14184750-14184769 None:intergenic 40.0%
! TTTAAGATCAGCAGCTGAAG+GGG - chr2.4:14184748-14184767 None:intergenic 40.0%
AGAGAAGAAGGTGGAAGAGA+AGG + chr2.4:14186025-14186044 MS.gene43071:CDS 45.0%
AGCAAAGGAAGAGAAGAAGG+TGG + chr2.4:14186016-14186035 MS.gene43071:CDS 45.0%
AGCAGCAAAGGAAGAGAAGA+AGG + chr2.4:14186013-14186032 MS.gene43071:CDS 45.0%
CAACTCAATGTTGCCATCCT+CGG - chr2.4:14185850-14185869 None:intergenic 45.0%
GAACATGGGTCTGTTTGGAT+TGG + chr2.4:14185105-14185124 MS.gene43071:intron 45.0%
GTTGCTCCTAAGTGAAGTCA+AGG + chr2.4:14185866-14185885 MS.gene43071:CDS 45.0%
TCTTCCACTGGCAATAAGCT+CGG - chr2.4:14185904-14185923 None:intergenic 45.0%
TGCCAGTGGAAGAGAAAAGT+TGG + chr2.4:14185911-14185930 MS.gene43071:CDS 45.0%
! AAGTTGGCTTCAGTTCCATC+CGG + chr2.4:14185927-14185946 MS.gene43071:CDS 45.0%
! AGCCAACTTTTCTCTTCCAC+TGG - chr2.4:14185916-14185935 None:intergenic 45.0%
!! AGCTTACTTGTTGGCCGTTT+TGG + chr2.4:14184710-14184729 MS.gene43071:CDS 45.0%
!! GCTTACTTGTTGGCCGTTTT+GGG + chr2.4:14184711-14184730 MS.gene43071:CDS 45.0%
CCTAAGTGAAGTCAAGGGCA+AGG + chr2.4:14185872-14185891 MS.gene43071:CDS 50.0%
CCTTGCCCTTGACTTCACTT+AGG - chr2.4:14185875-14185894 None:intergenic 50.0%
TTTGCCGAGCTTATTGCCAG+TGG + chr2.4:14185897-14185916 MS.gene43071:CDS 50.0%
! TCTTCCTTTGCTGCTTCAGC+AGG - chr2.4:14186008-14186027 None:intergenic 50.0%
!! GGATTGGTTGCCTCCCAAAA+CGG - chr2.4:14184727-14184746 None:intergenic 50.0%
!! TACTTGTTGGCCGTTTTGGG+AGG + chr2.4:14184714-14184733 MS.gene43071:CDS 50.0%
GATCAGCAGCTGAAGGGGAT+TGG - chr2.4:14184743-14184762 None:intergenic 55.0%
GTTGCTGTGTCTGCTGCAAC+TGG + chr2.4:14185960-14185979 MS.gene43071:CDS 55.0%
TGTAGTTGGAGTTGAGGCCG+AGG + chr2.4:14185830-14185849 MS.gene43071:intron 55.0%
TTGGCTTCAGTTCCATCCGG+TGG + chr2.4:14185930-14185949 MS.gene43071:CDS 55.0%
CACAGCAACAGCACCACCAC+CGG - chr2.4:14185949-14185968 None:intergenic 60.0%
GCTGTGTCTGCTGCAACTGG+AGG + chr2.4:14185963-14185982 MS.gene43071:CDS 60.0%
GCTTCAGTTCCATCCGGTGG+TGG + chr2.4:14185933-14185952 MS.gene43071:CDS 60.0%
GTGTCTGCTGCAACTGGAGG+AGG + chr2.4:14185966-14185985 MS.gene43071:CDS 60.0%
GTTGGAGTTGAGGCCGAGGA+TGG + chr2.4:14185834-14185853 MS.gene43071:intron 60.0%
TGCGCCTGCTGAAGCAGCAA+AGG + chr2.4:14186001-14186020 MS.gene43071:CDS 60.0%
GCAACAGCACCACCACCGGA+TGG - chr2.4:14185945-14185964 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 14184696 14186175 14184696 ID=MS.gene43071
chr2.4 mRNA 14184696 14186175 14184696 ID=MS.gene43071.t1;Parent=MS.gene43071
chr2.4 exon 14184696 14184783 14184696 ID=MS.gene43071.t1.exon1;Parent=MS.gene43071.t1
chr2.4 CDS 14184696 14184783 14184696 ID=cds.MS.gene43071.t1;Parent=MS.gene43071.t1
chr2.4 exon 14185835 14186061 14185835 ID=MS.gene43071.t1.exon2;Parent=MS.gene43071.t1
chr2.4 CDS 14185835 14186061 14185835 ID=cds.MS.gene43071.t1;Parent=MS.gene43071.t1
chr2.4 exon 14186149 14186175 14186149 ID=MS.gene43071.t1.exon3;Parent=MS.gene43071.t1
chr2.4 CDS 14186149 14186175 14186149 ID=cds.MS.gene43071.t1;Parent=MS.gene43071.t1
Gene Sequence

>MS.gene43071

ATGAAGATTATCGCAGCTTACTTGTTGGCCGTTTTGGGAGGCAACCAATCCCCTTCAGCTGCTGATCTTAAACAAATTCTTGGATCAGGTTCGTATAAATCCCAATTTAATCTTTAATCTTATTCATTTTGTGTTTTTTATTTTATTTTATTATTACGCATTTTTTGTTCGTTTTTCCGGTTTCGATGATTAGTATCCGAATTATTTTAGTAGATCCATAAGTAAAAGTGTAGAATATGAATGTACTTTCAATTAGAGATTGCGAGTATACTAAAGTTTAGTTTTTTCTTTTTTTGCAAAAAATAATCAATCCTAGACTGTGGGTATAATGAAGTGAATGTTTTTTGCTAATTTAATTCAAGTTTAGGATTTGTTTAATTGATAATCGGATCAATTAAAATCGATGAATGAACATGGGTCTGTTTGGATTGGACTCGTTTGAGCTTAACTGTTTGGGAGAATTTATATGAAAACATCTTATAACAATTTTTTTGCAAAAATAATTTTAATTCAAGTTTGGGTTGGGATTTGTTTAACTTTAATTCAAGTTTGGGATTTGTTTCACTGATATTCATTTCAATGAAAATTGATCAAAATATTGGTTTATTTGGATTGGACTTATTTGAGCTTATCTACTGTTAGACTGTTAAGGAGAATTTATGAAAACATACAATTTTTCATAAGTTTTTTTCAAATACAGCTTATATTAAAACAACTTATAGCAAATACAAAAACAAGTTAACATTTTTTTAATCTTTTGCTATTCAAATAGTTTATGCAAATTCATAGGCTCTTATCATGATATAAGTGCTTGTGCTATAAGCTGTAATAAGCTGTTTAATCAAACAGCCCCATATATTGGTTTGATTTTAATGCTTAGTTTTATACATTGTTTGGTAATATTTACGAAGTTTTCATTTTTACAGATGTATTAGGTTTTTGTTGAATTTTCAATCAGTAAATCGTGCATATGTTTGTCTGCTTTTTCTGTATATTTTTTTACTGTTGAAGGAAAAATGGTTTTCTTTTTCAGAAAATGATGTAATATTTTTTTACACTATGAAGATGAATTAAGAGATTAGAACATTCAATTTTGTGTGTAAAAAAAAGTGACTTTATTTTTGGGTCTATGATTGTAGTTGGAGTTGAGGCCGAGGATGGCAACATTGAGTTGCTCCTAAGTGAAGTCAAGGGCAAGGACTTTGCCGAGCTTATTGCCAGTGGAAGAGAAAAGTTGGCTTCAGTTCCATCCGGTGGTGGTGCTGTTGCTGTGTCTGCTGCAACTGGAGGAGGTGCTGCAGCTGCTGCGCCTGCTGAAGCAGCAAAGGAAGAGAAGAAGGTGGAAGAGAAGGAAGAATCCGATGATGTAAGTTTCAAAATTTATAGTAGTATTGTTTGTTACTTGGTAAATTCATGACTATGCTAATGTTTGCGTAATGTTATCTTTGTGCAGGATATGGGATTCAGCTTATTTGATTAA

Protein sequence

>MS.gene43071.t1

MKIIAAYLLAVLGGNQSPSAADLKQILGSVGVEAEDGNIELLLSEVKGKDFAELIASGREKLASVPSGGGAVAVSAATGGGAAAAAPAEAAKEEKKVEEKEESDDDMGFSLFD