Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013506.t1 | XP_013464220.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.70E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013506.t1 | P52855 | 68.3 | 63 | 20 | 0 | 1 | 63 | 1 | 63 | 6.7e-19 | 94.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013506.t1 | A0A072VJD3 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.9e-27 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43071 | MS.gene013506 | PPI |
| MS.gene042719 | MS.gene013506 | PPI |
| MS.gene06833 | MS.gene013506 | PPI |
| MS.gene013506 | MS.gene032942 | PPI |
| MS.gene013506 | MS.gene069339 | PPI |
| MS.gene013506 | MS.gene06617 | PPI |
| MS.gene013506 | MS.gene88792 | PPI |
| MS.gene013506 | MS.gene047578 | PPI |
| MS.gene013506 | MS.gene002442 | PPI |
| MS.gene073078 | MS.gene013506 | PPI |
| MS.gene009874 | MS.gene013506 | PPI |
| MS.gene013506 | MS.gene045633 | PPI |
| MS.gene44900 | MS.gene013506 | PPI |
| MS.gene013506 | MS.gene057237 | PPI |
| MS.gene013506 | MS.gene060483 | PPI |
| MS.gene013506 | MS.gene01091 | PPI |
| MS.gene013506 | MS.gene91826 | PPI |
| MS.gene013506 | MS.gene78764 | PPI |
| MS.gene013506 | MS.gene97876 | PPI |
| MS.gene013506 | MS.gene053952 | PPI |
| MS.gene013506 | MS.gene08521 | PPI |
| MS.gene013506 | MS.gene01425 | PPI |
| MS.gene013506 | MS.gene82009 | PPI |
| MS.gene013506 | MS.gene067331 | PPI |
| MS.gene013506 | MS.gene76615 | PPI |
| MS.gene013506 | MS.gene35379 | PPI |
| MS.gene013506 | MS.gene01952 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013506.t1 | MTR_3g092600 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.12e-74 | 215 |
| MS.gene013506.t1 | MTR_2g066750 | 98.182 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 1.07e-73 | 214 |
| MS.gene013506.t1 | MTR_8g024350 | 64.545 | 110 | 37 | 2 | 1 | 110 | 1 | 108 | 1.42e-33 | 112 |
| MS.gene013506.t1 | MTR_7g118060 | 62.712 | 59 | 22 | 0 | 1 | 59 | 1 | 59 | 1.00e-20 | 80.5 |
| MS.gene013506.t1 | MTR_7g005740 | 66.129 | 62 | 2 | 1 | 1 | 62 | 1 | 43 | 1.52e-19 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013506.t1 | AT5G24510 | 57.391 | 115 | 40 | 4 | 1 | 110 | 1 | 111 | 4.14e-36 | 119 |
| MS.gene013506.t1 | AT5G47700 | 57.658 | 111 | 45 | 1 | 2 | 110 | 3 | 113 | 7.99e-29 | 100 |
| MS.gene013506.t1 | AT5G47700 | 57.658 | 111 | 45 | 1 | 2 | 110 | 3 | 113 | 7.99e-29 | 100 |
| MS.gene013506.t1 | AT1G01100 | 60.185 | 108 | 42 | 1 | 4 | 110 | 5 | 112 | 4.26e-28 | 99.0 |
| MS.gene013506.t1 | AT1G01100 | 60.185 | 108 | 42 | 1 | 4 | 110 | 5 | 112 | 4.26e-28 | 99.0 |
| MS.gene013506.t1 | AT1G01100 | 60.185 | 108 | 42 | 1 | 4 | 110 | 5 | 112 | 4.26e-28 | 99.0 |
| MS.gene013506.t1 | AT1G01100 | 50.459 | 109 | 39 | 1 | 2 | 110 | 3 | 96 | 2.14e-27 | 96.7 |
| MS.gene013506.t1 | AT4G00810 | 54.955 | 111 | 48 | 1 | 2 | 110 | 3 | 113 | 2.87e-27 | 97.1 |
| MS.gene013506.t1 | AT4G00810 | 54.955 | 111 | 48 | 1 | 2 | 110 | 3 | 113 | 2.87e-27 | 97.1 |
Find 32 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGAGTTCCGGCGAAT+TGG | 0.294649 | 3.4:-84696695 | None:intergenic |
| GTCATCGTCACTTTCTTCCT+TGG | 0.329724 | 3.4:+84695449 | None:intergenic |
| GATAATAGACTTACGGTGAT+TGG | 0.337914 | 3.4:+84696631 | None:intergenic |
| ACGTCACCGTTGAATCTTAC+TGG | 0.348075 | 3.4:-84696489 | MS.gene013506:CDS |
| AAAATGAGTTCCGGCGAATT+GGG | 0.368280 | 3.4:-84696694 | MS.gene013506:CDS |
| TCTGAGCAAGCTTAGCGAAT+AGG | 0.392998 | 3.4:+84696461 | None:intergenic |
| GCCGTATCTGCACCCGCTGC+TGG | 0.401410 | 3.4:-84696382 | MS.gene013506:CDS |
| TCTGCACCCGCTGCTGGTGG+TGG | 0.439771 | 3.4:-84696376 | MS.gene013506:CDS |
| ATAATAGACTTACGGTGATT+GGG | 0.445449 | 3.4:+84696632 | None:intergenic |
| GCGGCTCCACCACCAGCAGC+GGG | 0.451994 | 3.4:+84696370 | None:intergenic |
| GAAGATCGGTACTTTGTTGA+AGG | 0.461021 | 3.4:-84696518 | MS.gene013506:CDS |
| ACCTGCCGCCGTTGAAAAGA+AGG | 0.476631 | 3.4:-84696335 | MS.gene013506:intron |
| TGCGGCTCCACCACCAGCAG+CGG | 0.489051 | 3.4:+84696369 | None:intergenic |
| TTGAACTCCGGCGCTGTTGG+TGG | 0.510539 | 3.4:-84696415 | MS.gene013506:CDS |
| ACAATTCACAAAATGAGTTC+CGG | 0.522809 | 3.4:-84696703 | None:intergenic |
| GGAAGAAAGTGACGATGACA+TGG | 0.523361 | 3.4:-84695445 | MS.gene013506:CDS |
| AGCGGCACCACCAACAGCGC+CGG | 0.527844 | 3.4:+84696408 | None:intergenic |
| ACCAGCAGCGGGTGCAGATA+CGG | 0.530473 | 3.4:+84696381 | None:intergenic |
| TCCATCGTCGTGGAGGATCA+AGG | 0.532980 | 3.4:+84696657 | None:intergenic |
| GCAAGCTTAGCGAATAGGCT+TGG | 0.542190 | 3.4:+84696466 | None:intergenic |
| TTTGTTTAGGCGGAGAAGAT+CGG | 0.543289 | 3.4:-84696532 | MS.gene013506:intron |
| GGCAGGTTCGGCAGCTGCTG+CGG | 0.548908 | 3.4:+84696351 | None:intergenic |
| GTAAATGCAGCCCAATTCGC+CGG | 0.571217 | 3.4:+84696684 | None:intergenic |
| ACCTTGATCCTCCACGACGA+TGG | 0.573503 | 3.4:-84696658 | MS.gene013506:CDS |
| GTATCTGCACCCGCTGCTGG+TGG | 0.603091 | 3.4:-84696379 | MS.gene013506:CDS |
| GCTTGGCCAGTAAGATTCAA+CGG | 0.629260 | 3.4:+84696483 | None:intergenic |
| TTGGGATTCCATCGTCGTGG+AGG | 0.650788 | 3.4:+84696650 | None:intergenic |
| GTGTTTGTAGGAGGAAGCCA+AGG | 0.654949 | 3.4:-84695466 | MS.gene013506:intron |
| TGATTGGGATTCCATCGTCG+TGG | 0.662981 | 3.4:+84696647 | None:intergenic |
| GGGTGCAGATACGGCAACAG+CGG | 0.665524 | 3.4:+84696390 | None:intergenic |
| GAAGAAAGTGACGATGACAT+GGG | 0.678372 | 3.4:-84695444 | MS.gene013506:CDS |
| ATCGTCGTGGAGGATCAAGG+TGG | 0.743990 | 3.4:+84696660 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTATATGTTTTTATCTTA+AGG | + | chr3.4:84695981-84696000 | None:intergenic | 10.0% |
| !! | ATGAAAATTTGTAGTTAATT+TGG | - | chr3.4:84696182-84696201 | MS.gene013506:intron | 15.0% |
| !! | AAAGTAAAAAATGTGGATAT+AGG | + | chr3.4:84696479-84696498 | None:intergenic | 20.0% |
| !! | AAGTAAAAAATGTGGATATA+GGG | + | chr3.4:84696478-84696497 | None:intergenic | 20.0% |
| !! | AATTGTGTTGAAATTAGAAA+CGG | - | chr3.4:84695882-84695901 | MS.gene013506:intron | 20.0% |
| !!! | ATATCCACATTTTTTACTTT+TGG | - | chr3.4:84696479-84696498 | MS.gene013506:CDS | 20.0% |
| !!! | ATTTTTCTTTTGTTACTGTT+TGG | - | chr3.4:84695554-84695573 | MS.gene013506:intron | 20.0% |
| !!! | TATCCACATTTTTTACTTTT+GGG | - | chr3.4:84696480-84696499 | MS.gene013506:CDS | 20.0% |
| !!! | TTTTTCTTTTGTTACTGTTT+GGG | - | chr3.4:84695555-84695574 | MS.gene013506:intron | 20.0% |
| ! | ATACCCAAAAGTAAAAAATG+TGG | + | chr3.4:84696486-84696505 | None:intergenic | 25.0% |
| ! | ATTGTGTTGAAATTAGAAAC+GGG | - | chr3.4:84695883-84695902 | MS.gene013506:intron | 25.0% |
| ! | TTAAATAAGCCAATCATAAC+TGG | - | chr3.4:84696452-84696471 | MS.gene013506:CDS | 25.0% |
| !! | AAACAATACCTTCTTTTCAA+CGG | + | chr3.4:84695792-84695811 | None:intergenic | 25.0% |
| !!! | GATTGTTGTTTTTCTTCATT+GGG | - | chr3.4:84696223-84696242 | MS.gene013506:intron | 25.0% |
| !!! | TAAGTTAACTTGTTTAGCTT+GGG | - | chr3.4:84696370-84696389 | MS.gene013506:CDS | 25.0% |
| !!! | TGATTGTTGTTTTTCTTCAT+TGG | - | chr3.4:84696222-84696241 | MS.gene013506:intron | 25.0% |
| !!! | TTAAGTTAACTTGTTTAGCT+TGG | - | chr3.4:84696369-84696388 | MS.gene013506:CDS | 25.0% |
| AATGTAAGCTTATGCATAAG+TGG | + | chr3.4:84696323-84696342 | None:intergenic | 30.0% | |
| AGATGCAAGTTCTACAAAAT+AGG | + | chr3.4:84696081-84696100 | None:intergenic | 30.0% | |
| ATAATAGACTTACGGTGATT+GGG | + | chr3.4:84695487-84695506 | None:intergenic | 30.0% | |
| ATGTAAGCTTATGCATAAGT+GGG | + | chr3.4:84696322-84696341 | None:intergenic | 30.0% | |
| GAAGAGAGATAATAGACTTA+CGG | + | chr3.4:84695495-84695514 | None:intergenic | 30.0% | |
| TCATTTCATACTGTGTTTGT+AGG | - | chr3.4:84696638-84696657 | MS.gene013506:intron | 30.0% | |
| TGTTAAATGAATGCTAGTTC+TGG | - | chr3.4:84695930-84695949 | MS.gene013506:intron | 30.0% | |
| ! | ATTTTGTAGAACTTGCATCT+AGG | - | chr3.4:84696081-84696100 | MS.gene013506:intron | 30.0% |
| ! | CGATTTGGAGATTATAGAAA+TGG | + | chr3.4:84695522-84695541 | None:intergenic | 30.0% |
| AATGAGATCTATGAGCGATT+TGG | + | chr3.4:84695537-84695556 | None:intergenic | 35.0% | |
| CTTCATTGGGATTTGAGAAT+TGG | - | chr3.4:84696236-84696255 | MS.gene013506:intron | 35.0% | |
| GATAATAGACTTACGGTGAT+TGG | + | chr3.4:84695488-84695507 | None:intergenic | 35.0% | |
| TGTAATGACGAGTTACTGAT+TGG | - | chr3.4:84695853-84695872 | MS.gene013506:intron | 35.0% | |
| TTTCATACTGTGTTTGTAGG+AGG | - | chr3.4:84696641-84696660 | MS.gene013506:intron | 35.0% | |
| ! | ATTTTGTGCTGTGATCAAGT+TGG | - | chr3.4:84696048-84696067 | MS.gene013506:intron | 35.0% |
| ! | TCTTTTGTTACTGTTTGGGT+TGG | - | chr3.4:84695559-84695578 | MS.gene013506:intron | 35.0% |
| ! | TTTTGTGCTGTGATCAAGTT+GGG | - | chr3.4:84696049-84696068 | MS.gene013506:intron | 35.0% |
| !!! | GTTTGGGTTGGTTTTTGTTT+AGG | - | chr3.4:84695571-84695590 | MS.gene013506:intron | 35.0% |
| ATGCAACTCAGAGCAAAATG+AGG | + | chr3.4:84696140-84696159 | None:intergenic | 40.0% | |
| GAAGAAAGTGACGATGACAT+GGG | - | chr3.4:84696672-84696691 | MS.gene013506:CDS | 40.0% | |
| GAAGATCGGTACTTTGTTGA+AGG | - | chr3.4:84695598-84695617 | MS.gene013506:intron | 40.0% | |
| GATATAGGGCCAGTTATGAT+TGG | + | chr3.4:84696464-84696483 | None:intergenic | 40.0% | |
| TCTGTTTAGCGACACTGTTT+GGG | + | chr3.4:84696425-84696444 | None:intergenic | 40.0% | |
| TTTGTTTAGGCGGAGAAGAT+CGG | - | chr3.4:84695584-84695603 | MS.gene013506:intron | 40.0% | |
| ! | CAATACCTTCTTTTCAACGG+CGG | + | chr3.4:84695789-84695808 | None:intergenic | 40.0% |
| ! | TTTGTGCTGTGATCAAGTTG+GGG | - | chr3.4:84696050-84696069 | MS.gene013506:intron | 40.0% |
| !! | GATGATCTCGTTTTGAACTC+CGG | - | chr3.4:84695689-84695708 | MS.gene013506:intron | 40.0% |
| !!! | TGGGTTGGTTTTTGTTTAGG+CGG | - | chr3.4:84695574-84695593 | MS.gene013506:intron | 40.0% |
| ACGTCACCGTTGAATCTTAC+TGG | - | chr3.4:84695627-84695646 | MS.gene013506:intron | 45.0% | |
| GCTTGGCCAGTAAGATTCAA+CGG | + | chr3.4:84695636-84695655 | None:intergenic | 45.0% | |
| GGAAGAAAGTGACGATGACA+TGG | - | chr3.4:84696671-84696690 | MS.gene013506:CDS | 45.0% | |
| GTCTGTTTAGCGACACTGTT+TGG | + | chr3.4:84696426-84696445 | None:intergenic | 45.0% | |
| TCTGAGCAAGCTTAGCGAAT+AGG | + | chr3.4:84695658-84695677 | None:intergenic | 45.0% | |
| ! | GTCATCGTCACTTTCTTCCT+TGG | + | chr3.4:84696670-84696689 | None:intergenic | 45.0% |
| GCAAGCTTAGCGAATAGGCT+TGG | + | chr3.4:84695653-84695672 | None:intergenic | 50.0% | |
| GTAAATGCAGCCCAATTCGC+CGG | + | chr3.4:84695435-84695454 | None:intergenic | 50.0% | |
| GTGTTTGTAGGAGGAAGCCA+AGG | - | chr3.4:84696650-84696669 | MS.gene013506:CDS | 50.0% | |
| TGATTGGGATTCCATCGTCG+TGG | + | chr3.4:84695472-84695491 | None:intergenic | 50.0% | |
| ACCTGCCGCCGTTGAAAAGA+AGG | - | chr3.4:84695781-84695800 | MS.gene013506:intron | 55.0% | |
| ACCTTGATCCTCCACGACGA+TGG | - | chr3.4:84695458-84695477 | MS.gene013506:CDS | 55.0% | |
| ATCGTCGTGGAGGATCAAGG+TGG | + | chr3.4:84695459-84695478 | None:intergenic | 55.0% | |
| TCCATCGTCGTGGAGGATCA+AGG | + | chr3.4:84695462-84695481 | None:intergenic | 55.0% | |
| TTGGGATTCCATCGTCGTGG+AGG | + | chr3.4:84695469-84695488 | None:intergenic | 55.0% | |
| ! | ACCTTCTTTTCAACGGCGGC+AGG | + | chr3.4:84695785-84695804 | None:intergenic | 55.0% |
| ! | CTTTTCAACGGCGGCAGGTT+CGG | + | chr3.4:84695780-84695799 | None:intergenic | 55.0% |
| !! | GTTTTGAACTCCGGCGCTGT+TGG | - | chr3.4:84695698-84695717 | MS.gene013506:intron | 55.0% |
| ACCAGCAGCGGGTGCAGATA+CGG | + | chr3.4:84695738-84695757 | None:intergenic | 60.0% | |
| GGGTGCAGATACGGCAACAG+CGG | + | chr3.4:84695729-84695748 | None:intergenic | 60.0% | |
| TTGAACTCCGGCGCTGTTGG+TGG | - | chr3.4:84695701-84695720 | MS.gene013506:intron | 60.0% | |
| GTATCTGCACCCGCTGCTGG+TGG | - | chr3.4:84695737-84695756 | MS.gene013506:intron | 65.0% | |
| AGCGGCACCACCAACAGCGC+CGG | + | chr3.4:84695711-84695730 | None:intergenic | 70.0% | |
| GCCGTATCTGCACCCGCTGC+TGG | - | chr3.4:84695734-84695753 | MS.gene013506:intron | 70.0% | |
| GGCAGGTTCGGCAGCTGCTG+CGG | + | chr3.4:84695768-84695787 | None:intergenic | 70.0% | |
| TCTGCACCCGCTGCTGGTGG+TGG | - | chr3.4:84695740-84695759 | MS.gene013506:intron | 70.0% | |
| TGCGGCTCCACCACCAGCAG+CGG | + | chr3.4:84695750-84695769 | None:intergenic | 70.0% | |
| GCGGCTCCACCACCAGCAGC+GGG | + | chr3.4:84695749-84695768 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 84695425 | 84696713 | 84695425 | ID=MS.gene013506 |
| chr3.4 | mRNA | 84695425 | 84696713 | 84695425 | ID=MS.gene013506.t1;Parent=MS.gene013506 |
| chr3.4 | exon | 84696645 | 84696713 | 84696645 | ID=MS.gene013506.t1.exon1;Parent=MS.gene013506.t1 |
| chr3.4 | CDS | 84696645 | 84696713 | 84696645 | ID=cds.MS.gene013506.t1;Parent=MS.gene013506.t1 |
| chr3.4 | exon | 84696336 | 84696545 | 84696336 | ID=MS.gene013506.t1.exon2;Parent=MS.gene013506.t1 |
| chr3.4 | CDS | 84696336 | 84696545 | 84696336 | ID=cds.MS.gene013506.t1;Parent=MS.gene013506.t1 |
| chr3.4 | exon | 84695425 | 84695478 | 84695425 | ID=MS.gene013506.t1.exon3;Parent=MS.gene013506.t1 |
| chr3.4 | CDS | 84695425 | 84695478 | 84695425 | ID=cds.MS.gene013506.t1;Parent=MS.gene013506.t1 |
| Gene Sequence |
| Protein sequence |
