AlfalfaGEDB | MS.gene88792

M. sativa cultivar XinJiangDaYe / MS.gene88792

KEGG: K02942
GO: -
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88792.t1	XP_024638676.1	90.9	66	6	0	1	66	1	66	2.50E-25	125.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88792.t1	P52855	63.5	63	23	0	1	63	1	63	6.2e-17	88.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88792.t1	A0A396I1J6	90.9	66	6	0	1	66	1	66	1.8e-25	125.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene002442	MS.gene88792	PPI
MS.gene88792	MS.gene35379	PPI
MS.gene88792	MS.gene01952	PPI
MS.gene060483	MS.gene88792	PPI
MS.gene06617	MS.gene88792	PPI
MS.gene01952	MS.gene88792	PPI
MS.gene43071	MS.gene88792	PPI
MS.gene08521	MS.gene88792	PPI
MS.gene88792	MS.gene032942	PPI
MS.gene042719	MS.gene88792	PPI
MS.gene06833	MS.gene88792	PPI
MS.gene067331	MS.gene88792	PPI
MS.gene013506	MS.gene88792	PPI
MS.gene88792	MS.gene06617	PPI
MS.gene76615	MS.gene88792	PPI
MS.gene057237	MS.gene88792	PPI
MS.gene88792	MS.gene047578	PPI
MS.gene82009	MS.gene88792	PPI
MS.gene073078	MS.gene88792	PPI
MS.gene045633	MS.gene88792	PPI
MS.gene069339	MS.gene88792	PPI
MS.gene88792	MS.gene045633	PPI
MS.gene88792	MS.gene057237	PPI
MS.gene009874	MS.gene88792	PPI
MS.gene88792	MS.gene01091	PPI
MS.gene44900	MS.gene88792	PPI
MS.gene88792	MS.gene91826	PPI
MS.gene88792	MS.gene78764	PPI
MS.gene88792	MS.gene97876	PPI
MS.gene88792	MS.gene053952	PPI
MS.gene88792	MS.gene01425	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88792.t1	MTR_3g092600	93.443	61	4	0	1	61	1	61	2.67e-35	118
MS.gene88792.t1	MTR_2g066750	93.443	61	4	0	1	61	1	61	2.67e-35	118
MS.gene88792.t1	MTR_8g024350	63.889	72	25	1	1	72	1	71	1.74e-25	93.6
MS.gene88792.t1	MTR_7g118060	61.017	59	23	0	1	59	1	59	3.48e-19	77.8
MS.gene88792.t1	MTR_7g005740	61.290	62	5	1	1	62	1	43	7.02e-16	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88792.t1	AT5G24510	64.789	71	24	1	1	71	1	70	1.41e-26	96.7
MS.gene88792.t1	AT5G47700	63.158	57	21	0	5	61	6	62	1.99e-20	80.9
MS.gene88792.t1	AT5G47700	63.158	57	21	0	5	61	6	62	1.99e-20	80.9
MS.gene88792.t1	AT1G01100	61.404	57	22	0	5	61	6	62	3.43e-20	79.7
MS.gene88792.t1	AT1G01100	61.404	57	22	0	5	61	6	62	5.47e-20	79.7
MS.gene88792.t1	AT1G01100	61.404	57	22	0	5	61	6	62	5.47e-20	79.7
MS.gene88792.t1	AT1G01100	61.404	57	22	0	5	61	6	62	5.47e-20	79.7
MS.gene88792.t1	AT4G00810	59.649	57	23	0	5	61	6	62	2.87e-19	77.8
MS.gene88792.t1	AT4G00810	59.649	57	23	0	5	61	6	62	2.87e-19	77.8

Find 39 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGTATTTGCACTTGCTTC+TGG	0.230104	5.4:-55355874	MS.gene88792:CDS
AGATAGTAGATTGCAAGTTT+TGG	0.304388	5.4:+55355696	None:intergenic
GTCATCGTCACTTTCTTCCT+TGG	0.329724	5.4:+55355802	None:intergenic
ACGTCACTGTTGAATCTTAC+TGG	0.343339	5.4:-55355981	MS.gene88792:CDS
GATAGCAGACTTACGGTGAT+AGG	0.389185	5.4:+55356118	None:intergenic
CAAAATGAGTTCCAACGAAT+CGG	0.399254	5.4:-55356182	None:intergenic
TTTGCACTTGCTTCTGGAGG+TGG	0.412215	5.4:-55355868	MS.gene88792:CDS
CCAGACTAGGACTCCTTTCA+AGG	0.415086	5.4:-55355731	MS.gene88792:CDS
CCTTGAAAGGAGTCCTAGTC+TGG	0.418177	5.4:+55355731	None:intergenic
TCCAGAAGCAAGTGCAAATA+CGG	0.439030	5.4:+55355873	None:intergenic
TCTGAGCAAGCTTAGCGAAT+AGG	0.441352	5.4:+55355953	None:intergenic
AACTGCAGCCGTTGAAAAGA+AGG	0.478736	5.4:-55355831	MS.gene88792:CDS
GAAGATCGGTACTTTGTTGA+AGG	0.484661	5.4:-55356010	MS.gene88792:CDS
AAAATGAGTTCCAACGAATC+GGG	0.497361	5.4:-55356181	MS.gene88792:CDS
CGGCTGCAGTTCGGTTGTTG+CGG	0.504668	5.4:+55355843	None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	5.4:-55355907	MS.gene88792:CDS
ACTGTTGAATCTTACTGGCC+AGG	0.517027	5.4:-55355976	MS.gene88792:CDS
TAGTTTGTTGTAGCCAGACT+AGG	0.521961	5.4:-55355744	MS.gene88792:CDS
GGAAGAAAGTGACGATGACA+TGG	0.523361	5.4:-55355798	MS.gene88792:CDS
GCAAGCTTAGCGAATAGGCC+TGG	0.531593	5.4:+55355958	None:intergenic
TTTGTTTAGGCGAAGAAGAT+CGG	0.532771	5.4:-55356024	MS.gene88792:intron
TCCATCGTCGTGGAGGATCA+AGG	0.532980	5.4:+55356144	None:intergenic
GGCGGCACCACCAACAGCGC+CGG	0.535107	5.4:+55355900	None:intergenic
ATAGCAGACTTACGGTGATA+GGG	0.561399	5.4:+55356119	None:intergenic
GTAAATGCAACCCGATTCGT+TGG	0.564773	5.4:+55356171	None:intergenic
ACCTTGATCCTCCACGACGA+TGG	0.573503	5.4:-55356145	MS.gene88792:CDS
TGAAAAGAAGGAGGAAGCCA+AGG	0.574510	5.4:-55355819	MS.gene88792:CDS
CAGACTAGGACTCCTTTCAA+GGG	0.583004	5.4:-55355730	MS.gene88792:CDS
AGATTCAACAGTGACGTTAG+CGG	0.589927	5.4:+55355987	None:intergenic
GTATTTGCACTTGCTTCTGG+AGG	0.593602	5.4:-55355871	MS.gene88792:CDS
AGCAAGTGCAAATACGGCAA+CGG	0.622430	5.4:+55355879	None:intergenic
AGACTAGGACTCCTTTCAAG+GGG	0.655500	5.4:-55355729	MS.gene88792:CDS
AAGTGCAAATACGGCAACGG+CGG	0.656796	5.4:+55355882	None:intergenic
TGATAGGGATTCCATCGTCG+TGG	0.665821	5.4:+55356134	None:intergenic
TAGGGATTCCATCGTCGTGG+AGG	0.668252	5.4:+55356137	None:intergenic
TGCAGCCGTTGAAAAGAAGG+AGG	0.676132	5.4:-55355828	MS.gene88792:CDS
GAAGAAAGTGACGATGACAT+GGG	0.678372	5.4:-55355797	MS.gene88792:CDS
GACTAGGACTCCTTTCAAGG+GGG	0.714058	5.4:-55355728	MS.gene88792:CDS
ATCGTCGTGGAGGATCAAGG+TGG	0.743990	5.4:+55356147	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACAGAAATAGAATTGAATA+GGG	+	chr5.4:55355760-55355779	None:intergenic	20.0%
!!	TTACAGAAATAGAATTGAAT+AGG	+	chr5.4:55355761-55355780	None:intergenic	20.0%
!!!	ATTTTTCTTTGTTACTGTTT+GGG	-	chr5.4:55355804-55355823	MS.gene88792:CDS	20.0%
!!	CATTTTTCTTTGTTACTGTT+TGG	-	chr5.4:55355803-55355822	MS.gene88792:CDS	25.0%
!	AGATAGTAGATTGCAAGTTT+TGG	+	chr5.4:55356160-55356179	None:intergenic	30.0%
!!	TACTGTTTGGGTTTTTGTTT+AGG	-	chr5.4:55355816-55355835	MS.gene88792:CDS	30.0%
	AATGAGATCTATGAGCGATT+TGG	+	chr5.4:55355787-55355806	None:intergenic	35.0%
	TTTGTTTAGGCGAAGAAGAT+CGG	-	chr5.4:55355829-55355848	MS.gene88792:CDS	35.0%
	ACGTCACTGTTGAATCTTAC+TGG	-	chr5.4:55355872-55355891	MS.gene88792:CDS	40.0%
	AGATTCAACAGTGACGTTAG+CGG	+	chr5.4:55355869-55355888	None:intergenic	40.0%
	ATAGCAGACTTACGGTGATA+GGG	+	chr5.4:55355737-55355756	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr5.4:55356056-55356075	MS.gene88792:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr5.4:55355843-55355862	MS.gene88792:CDS	40.0%
	GAATAGGGATAGCAGACTTA+CGG	+	chr5.4:55355745-55355764	None:intergenic	40.0%
	TCCAGAAGCAAGTGCAAATA+CGG	+	chr5.4:55355983-55356002	None:intergenic	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr5.4:55355934-55355953	MS.gene88792:CDS	40.0%
!!	TAGTTTGTTGTAGCCAGACT+AGG	-	chr5.4:55356109-55356128	MS.gene88792:intron	40.0%
	AACTGCAGCCGTTGAAAAGA+AGG	-	chr5.4:55356022-55356041	MS.gene88792:intron	45.0%
	ACTGTTGAATCTTACTGGCC+AGG	-	chr5.4:55355877-55355896	MS.gene88792:CDS	45.0%
	AGACTAGGACTCCTTTCAAG+GGG	-	chr5.4:55356124-55356143	MS.gene88792:intron	45.0%
	AGCAAGTGCAAATACGGCAA+CGG	+	chr5.4:55355977-55355996	None:intergenic	45.0%
	CAGACTAGGACTCCTTTCAA+GGG	-	chr5.4:55356123-55356142	MS.gene88792:intron	45.0%
	GATAGCAGACTTACGGTGAT+AGG	+	chr5.4:55355738-55355757	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr5.4:55356055-55356074	MS.gene88792:intron	45.0%
	GTATTTGCACTTGCTTCTGG+AGG	-	chr5.4:55355982-55356001	MS.gene88792:CDS	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr5.4:55355903-55355922	None:intergenic	45.0%
	TGAAAAGAAGGAGGAAGCCA+AGG	-	chr5.4:55356034-55356053	MS.gene88792:intron	45.0%
!	GGCTTCCTCCTTCTTTTCAA+CGG	+	chr5.4:55356033-55356052	None:intergenic	45.0%
!	GTAAATGCAACCCGATTCGT+TGG	+	chr5.4:55355685-55355704	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr5.4:55356054-55356073	None:intergenic	45.0%
!	TCTTTTCAACGGCTGCAGTT+CGG	+	chr5.4:55356022-55356041	None:intergenic	45.0%
	AAGTGCAAATACGGCAACGG+CGG	+	chr5.4:55355974-55355993	None:intergenic	50.0%
	CCAGACTAGGACTCCTTTCA+AGG	-	chr5.4:55356122-55356141	MS.gene88792:intron	50.0%
	CCTTGAAAGGAGTCCTAGTC+TGG	+	chr5.4:55356125-55356144	None:intergenic	50.0%
	GACTAGGACTCCTTTCAAGG+GGG	-	chr5.4:55356125-55356144	MS.gene88792:intron	50.0%
	GCCGTATTTGCACTTGCTTC+TGG	-	chr5.4:55355979-55355998	MS.gene88792:CDS	50.0%
	TGATAGGGATTCCATCGTCG+TGG	+	chr5.4:55355722-55355741	None:intergenic	50.0%
	TGCAGCCGTTGAAAAGAAGG+AGG	-	chr5.4:55356025-55356044	MS.gene88792:intron	50.0%
!	TTTGCACTTGCTTCTGGAGG+TGG	-	chr5.4:55355985-55356004	MS.gene88792:CDS	50.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr5.4:55355708-55355727	MS.gene88792:CDS	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr5.4:55355709-55355728	None:intergenic	55.0%
	GATTCAGCCACCCCCTTGAA+AGG	+	chr5.4:55356138-55356157	None:intergenic	55.0%
	GCAAGCTTAGCGAATAGGCC+TGG	+	chr5.4:55355898-55355917	None:intergenic	55.0%
	TAGGACTCCTTTCAAGGGGG+TGG	-	chr5.4:55356128-55356147	MS.gene88792:intron	55.0%
	TAGGGATTCCATCGTCGTGG+AGG	+	chr5.4:55355719-55355738	None:intergenic	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr5.4:55355712-55355731	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr5.4:55355943-55355962	MS.gene88792:CDS	55.0%
	CGGCTGCAGTTCGGTTGTTG+CGG	+	chr5.4:55356013-55356032	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr5.4:55355946-55355965	MS.gene88792:CDS	60.0%
	GGCGGCACCACCAACAGCGC+CGG	+	chr5.4:55355956-55355975	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	55355675	55356200	55355675	ID=MS.gene88792
chr5.4	mRNA	55355675	55356200	55355675	ID=MS.gene88792.t1;Parent=MS.gene88792
chr5.4	exon	55356132	55356200	55356132	ID=MS.gene88792.t1.exon1;Parent=MS.gene88792.t1
chr5.4	CDS	55356132	55356200	55356132	ID=cds.MS.gene88792.t1;Parent=MS.gene88792.t1
chr5.4	exon	55355675	55356037	55355675	ID=MS.gene88792.t1.exon2;Parent=MS.gene88792.t1
chr5.4	CDS	55355675	55356037	55355675	ID=cds.MS.gene88792.t1;Parent=MS.gene88792.t1

Gene Sequence

>MS.gene88792

ATGAGTTCCAACGAATCGGGTTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCTATCACCGTAAGTCTGCTATCCCTATTCAATTCTATTTCTGTAATCTCCAAATCGCTCATAGATCTCATTTTTCTTTGTTACTGTTTGGGTTTTTGTTTAGGCGAAGAAGATCGGTACTTTGTTGAAGGCCGCTAACGTCACTGTTGAATCTTACTGGCCAGGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCGCCGTTGCCGTATTTGCACTTGCTTCTGGAGGTGGAGCCGCAACAACCGAACTGCAGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAAGTTTCTATGTATAGTTTGTTGTAGCCAGACTAGGACTCCTTTCAAGGGGGTGGCTGAATCCAAAACTTGCAATCTACTATCTCTTTATAAATTGTTTATCTAA

Protein sequence

>MS.gene88792.t1

MSSNESGCIYATLILHDDGIPITAKKIGTLLKAANVTVESYWPGLFAKLAQSKNVDDLVLNSGAVGGAAVAVFALASGGGAATTELQPLKRRRKPRKKVTMTWALVCLIKFLCIVCCSQTRTPFKGVAESKTCNLLSLYKLFI