AlfalfaGEDB | MS.gene01952

M. sativa cultivar XinJiangDaYe / MS.gene01952

KEGG: K02942
GO: -
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01952.t1	XP_013464220.1	92.3	65	5	0	1	65	1	65	3.00E-25	124.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01952.t1	P52855	63.5	63	23	0	1	63	1	63	4.3e-17	88.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01952.t1	A0A072VJD3	92.3	65	5	0	1	65	1	65	2.1e-25	124.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01952	MS.gene032942	PPI
MS.gene76615	MS.gene01952	PPI
MS.gene057237	MS.gene01952	PPI
MS.gene88792	MS.gene01952	PPI
MS.gene01952	MS.gene35379	PPI
MS.gene43071	MS.gene01952	PPI
MS.gene01952	MS.gene88792	PPI
MS.gene01952	MS.gene047578	PPI
MS.gene06833	MS.gene01952	PPI
MS.gene067331	MS.gene01952	PPI
MS.gene045633	MS.gene01952	PPI
MS.gene069339	MS.gene01952	PPI
MS.gene01952	MS.gene06617	PPI
MS.gene44900	MS.gene01952	PPI
MS.gene82009	MS.gene01952	PPI
MS.gene01952	MS.gene01091	PPI
MS.gene073078	MS.gene01952	PPI
MS.gene01952	MS.gene78764	PPI
MS.gene009874	MS.gene01952	PPI
MS.gene01952	MS.gene045633	PPI
MS.gene01952	MS.gene057237	PPI
MS.gene002442	MS.gene01952	PPI
MS.gene060483	MS.gene01952	PPI
MS.gene06617	MS.gene01952	PPI
MS.gene01952	MS.gene91826	PPI
MS.gene01952	MS.gene97876	PPI
MS.gene01952	MS.gene053952	PPI
MS.gene01952	MS.gene01425	PPI
MS.gene08521	MS.gene01952	PPI
MS.gene042719	MS.gene01952	PPI
MS.gene013506	MS.gene01952	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01952.t1	MTR_3g092600	95.082	61	3	0	1	61	1	61	8.79e-37	120
MS.gene01952.t1	MTR_2g066750	95.082	61	3	0	1	61	1	61	8.79e-37	120
MS.gene01952.t1	MTR_8g024350	65.753	73	24	1	1	73	1	72	6.61e-27	95.5
MS.gene01952.t1	MTR_7g118060	59.322	59	24	0	1	59	1	59	2.30e-19	76.6
MS.gene01952.t1	MTR_7g005740	64.516	62	3	2	1	62	1	43	6.31e-18	74.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01952.t1	AT5G24510	52.577	97	42	3	1	97	1	93	3.94e-28	98.6
MS.gene01952.t1	AT5G47700	60.345	58	23	0	4	61	5	62	7.32e-21	80.5
MS.gene01952.t1	AT5G47700	60.345	58	23	0	4	61	5	62	7.32e-21	80.5
MS.gene01952.t1	AT1G01100	58.621	58	24	0	4	61	5	62	1.50e-20	79.0
MS.gene01952.t1	AT1G01100	58.621	58	24	0	4	61	5	62	2.49e-20	79.0
MS.gene01952.t1	AT1G01100	58.621	58	24	0	4	61	5	62	2.49e-20	79.0
MS.gene01952.t1	AT1G01100	58.621	58	24	0	4	61	5	62	2.49e-20	79.0
MS.gene01952.t1	AT4G00810	58.621	58	24	0	4	61	5	62	3.71e-20	78.6
MS.gene01952.t1	AT4G00810	58.621	58	24	0	4	61	5	62	3.71e-20	78.6

Find 31 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGATCTTCTCTTCGATGTT+TGG	0.215978	2.2:-59996332	None:intergenic
CGATCTTCTCTTCGATGTTT+GGG	0.265122	2.2:-59996331	None:intergenic
GTCATCCTCACTTTCTTCCT+TGG	0.284207	2.2:-59996559	None:intergenic
CAAAATGAGTTCCGGAGAAT+TGG	0.289659	2.2:+59996268	None:intergenic
GCCGTATCTGCACCTGTTGC+TGG	0.332352	2.2:+59996482	MS.gene01952:CDS
ACGTCACCGTTGAATCTTAC+TGG	0.348075	2.2:+59996375	MS.gene01952:CDS
TCTGAGCAAGCTTAGCGAAT+AGG	0.392998	2.2:-59996403	None:intergenic
AAAATGAGTTCCGGAGAATT+GGG	0.430121	2.2:+59996269	None:intergenic
GAAGATCGGCACTTTGTTGA+AGG	0.430819	2.2:+59996346	MS.gene01952:CDS
GCGGCTCCACCACCAGCAAC+AGG	0.449126	2.2:-59996494	None:intergenic
TCTGCACCTGTTGCTGGTGG+TGG	0.462388	2.2:+59996488	MS.gene01952:CDS
GGCAGGTTCGGCTGCTGTTG+CGG	0.503488	2.2:-59996513	None:intergenic
ACAATTCACAAAATGAGTTC+CGG	0.509067	2.2:+59996260	None:intergenic
ATAAATGCAGCCCAATTCTC+CGG	0.526621	2.2:-59996279	None:intergenic
GCAAGCTTAGCGAATAGGCT+TGG	0.542190	2.2:-59996398	None:intergenic
TGTTTGGGATTCCATCGTCA+TGG	0.556483	2.2:-59996316	None:intergenic
ACCAGCAACAGGTGCAGATA+CGG	0.561513	2.2:-59996483	None:intergenic
TCCATCGTCATGGAGGATCA+AGG	0.576194	2.2:-59996306	None:intergenic
CCAAACATCGAAGAGAAGAT+CGG	0.576478	2.2:+59996332	MS.gene01952:CDS
GCCGCCGTTGAAAAGAAAAG+AGG	0.578625	2.2:+59996533	MS.gene01952:CDS
GGCGGCACCACCGATAGTGC+CGG	0.582079	2.2:-59996456	None:intergenic
GGAAACCAAGGAAGAAAGTG+AGG	0.603561	2.2:+59996554	MS.gene01952:CDS
ACCTTGATCCTCCATGACGA+TGG	0.606345	2.2:+59996305	MS.gene01952:CDS
GCTTGGCCAGTAAGATTCAA+CGG	0.629260	2.2:-59996381	None:intergenic
TTGAACTCCGGCACTATCGG+TGG	0.629987	2.2:+59996449	MS.gene01952:CDS
GTATCTGCACCTGTTGCTGG+TGG	0.632454	2.2:+59996485	MS.gene01952:CDS
GAAAAGAAAAGAGGAAACCA+AGG	0.641863	2.2:+59996542	MS.gene01952:CDS
AGGTGCAGATACGGCAACGG+CGG	0.646368	2.2:-59996474	None:intergenic
AACAGGTGCAGATACGGCAA+CGG	0.662101	2.2:-59996477	None:intergenic
TTGGGATTCCATCGTCATGG+AGG	0.683729	2.2:-59996313	None:intergenic
ATCGTCATGGAGGATCAAGG+TGG	0.748536	2.2:-59996303	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTTCCTCTTTTCTTTTCAA+CGG	-	chr2.2:59996540-59996559	None:intergenic	30.0%
	GAAAAGAAAAGAGGAAACCA+AGG	+	chr2.2:59996542-59996561	MS.gene01952:CDS	35.0%
	ATAAATGCAGCCCAATTCTC+CGG	-	chr2.2:59996282-59996301	None:intergenic	40.0%
	CCAAACATCGAAGAGAAGAT+CGG	+	chr2.2:59996332-59996351	MS.gene01952:CDS	40.0%
	CGATCTTCTCTTCGATGTTT+GGG	-	chr2.2:59996334-59996353	None:intergenic	40.0%
!	TCCTCTTTTCTTTTCAACGG+CGG	-	chr2.2:59996537-59996556	None:intergenic	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	+	chr2.2:59996437-59996456	MS.gene01952:CDS	40.0%
	ACGTCACCGTTGAATCTTAC+TGG	+	chr2.2:59996375-59996394	MS.gene01952:CDS	45.0%
	CCGATCTTCTCTTCGATGTT+TGG	-	chr2.2:59996335-59996354	None:intergenic	45.0%
	GAAGATCGGCACTTTGTTGA+AGG	+	chr2.2:59996346-59996365	MS.gene01952:CDS	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	-	chr2.2:59996384-59996403	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	-	chr2.2:59996406-59996425	None:intergenic	45.0%
!	TGTTTGGGATTCCATCGTCA+TGG	-	chr2.2:59996319-59996338	None:intergenic	45.0%
!!	GTTTTGAACTCCGGCACTAT+CGG	+	chr2.2:59996446-59996465	MS.gene01952:CDS	45.0%
	AACAGGTGCAGATACGGCAA+CGG	-	chr2.2:59996480-59996499	None:intergenic	50.0%
	ACCAGCAACAGGTGCAGATA+CGG	-	chr2.2:59996486-59996505	None:intergenic	50.0%
	ACCTTGATCCTCCATGACGA+TGG	+	chr2.2:59996305-59996324	MS.gene01952:CDS	50.0%
	ATCGTCATGGAGGATCAAGG+TGG	-	chr2.2:59996306-59996325	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	-	chr2.2:59996401-59996420	None:intergenic	50.0%
	GCCGCCGTTGAAAAGAAAAG+AGG	+	chr2.2:59996533-59996552	MS.gene01952:CDS	50.0%
	TCCATCGTCATGGAGGATCA+AGG	-	chr2.2:59996309-59996328	None:intergenic	50.0%
	TTGGGATTCCATCGTCATGG+AGG	-	chr2.2:59996316-59996335	None:intergenic	50.0%
!	CTTTTCTTTTCAACGGCGGC+AGG	-	chr2.2:59996533-59996552	None:intergenic	50.0%
	GTATCTGCACCTGTTGCTGG+TGG	+	chr2.2:59996485-59996504	MS.gene01952:CDS	55.0%
	TTGAACTCCGGCACTATCGG+TGG	+	chr2.2:59996449-59996468	MS.gene01952:CDS	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	-	chr2.2:59996528-59996547	None:intergenic	55.0%
	AGGTGCAGATACGGCAACGG+CGG	-	chr2.2:59996477-59996496	None:intergenic	60.0%
	GCCGTATCTGCACCTGTTGC+TGG	+	chr2.2:59996482-59996501	MS.gene01952:CDS	60.0%
	TCTGCACCTGTTGCTGGTGG+TGG	+	chr2.2:59996488-59996507	MS.gene01952:CDS	60.0%
	GGCAGGTTCGGCTGCTGTTG+CGG	-	chr2.2:59996516-59996535	None:intergenic	65.0%
	GCGGCTCCACCACCAGCAAC+AGG	-	chr2.2:59996497-59996516	None:intergenic	70.0%
	GGCGGCACCACCGATAGTGC+CGG	-	chr2.2:59996459-59996478	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	59996272	59996574	59996272	ID=MS.gene01952
chr2.2	mRNA	59996272	59996574	59996272	ID=MS.gene01952.t1;Parent=MS.gene01952
chr2.2	exon	59996272	59996574	59996272	ID=MS.gene01952.t1.exon1;Parent=MS.gene01952.t1
chr2.2	CDS	59996272	59996574	59996272	ID=cds.MS.gene01952.t1;Parent=MS.gene01952.t1

Gene Sequence	>MS.gene01952 ATGAGTTCCGGAGAATTGGGCTGCATTTATGCCACCTTGATCCTCCATGACGATGGAATCCCAAACATCGAAGAGAAGATCGGCACTTTGTTGAAGGCTGCTAACGTCACCGTTGAATCTTACTGGCCAAGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCACTATCGGTGGTGCCGCCGTTGCCGTATCTGCACCTGTTGCTGGTGGTGGAGCCGCAACAGCAGCCGAACCTGCCGCCGTTGAAAAGAAAAGAGGAAACCAAGGAAGAAAGTGA
Protein sequence	>MS.gene01952.t1 MSSGELGCIYATLILHDDGIPNIEEKIGTLLKAANVTVESYWPSLFAKLAQSKNVDDLVLNSGTIGGAAVAVSAPVAGGGAATAAEPAAVEKKRGNQGRK