Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012159.t1 | XP_003629214.1 | 91.5 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 1.40E-208 | 735.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012159.t1 | Q9SHX9 | 30.0 | 353 | 221 | 9 | 6 | 353 | 3 | 334 | 8.1e-32 | 139.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012159.t1 | G7LA99 | 91.5 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 1.0e-208 | 735.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012159 | MS.gene037197 | PPI |
| MS.gene012159 | MS.gene012160 | PPI |
| MS.gene012159 | MS.gene017582 | PPI |
| MS.gene012159 | MS.gene39445 | PPI |
| MS.gene012159 | MS.gene064059 | PPI |
| MS.gene012159 | MS.gene28903 | PPI |
| MS.gene012159 | MS.gene08770 | PPI |
| MS.gene012159 | MS.gene025209 | PPI |
| MS.gene64423 | MS.gene012159 | PPI |
| MS.gene012159 | MS.gene64423 | PPI |
| MS.gene012159 | MS.gene44190 | PPI |
| MS.gene012159 | MS.gene28904 | PPI |
| MS.gene012159 | MS.gene66307 | PPI |
| MS.gene037197 | MS.gene012159 | PPI |
| MS.gene30094 | MS.gene012159 | PPI |
| MS.gene012159 | MS.gene44191 | PPI |
| MS.gene012159 | MS.gene30094 | PPI |
| MS.gene91760 | MS.gene012159 | PPI |
| MS.gene012159 | MS.gene017581 | PPI |
| MS.gene23497 | MS.gene012159 | PPI |
| MS.gene012159 | MS.gene023531 | PPI |
| MS.gene012159 | MS.gene23495 | PPI |
| MS.gene012159 | MS.gene018718 | PPI |
| MS.gene012159 | MS.gene049886 | PPI |
| MS.gene012159 | MS.gene97013 | PPI |
| MS.gene44190 | MS.gene012159 | PPI |
| MS.gene012159 | MS.gene23497 | PPI |
| MS.gene23495 | MS.gene012159 | PPI |
| MS.gene012159 | MS.gene91760 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012159.t1 | MTR_8g074640 | 91.534 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 0.0 | 705 |
| MS.gene012159.t1 | MTR_8g074610 | 76.842 | 380 | 84 | 4 | 1 | 378 | 20 | 397 | 0.0 | 591 |
| MS.gene012159.t1 | MTR_2g041510 | 75.853 | 381 | 80 | 5 | 1 | 378 | 21 | 392 | 0.0 | 556 |
| MS.gene012159.t1 | MTR_2g041510 | 75.853 | 381 | 80 | 5 | 1 | 378 | 1 | 372 | 0.0 | 556 |
| MS.gene012159.t1 | MTR_5g079830 | 35.593 | 354 | 179 | 12 | 1 | 340 | 1 | 319 | 2.91e-52 | 179 |
| MS.gene012159.t1 | MTR_6g092570 | 34.400 | 375 | 199 | 11 | 2 | 364 | 4 | 343 | 9.97e-50 | 171 |
| MS.gene012159.t1 | MTR_8g085760 | 33.684 | 380 | 201 | 12 | 2 | 354 | 9 | 364 | 8.28e-49 | 169 |
| MS.gene012159.t1 | MTR_7g096720 | 33.146 | 356 | 193 | 11 | 8 | 340 | 15 | 348 | 3.17e-45 | 160 |
| MS.gene012159.t1 | MTR_4g088925 | 31.830 | 377 | 193 | 12 | 14 | 364 | 34 | 372 | 6.50e-40 | 145 |
| MS.gene012159.t1 | MTR_1g026850 | 29.723 | 397 | 224 | 12 | 1 | 376 | 1 | 363 | 6.31e-36 | 134 |
| MS.gene012159.t1 | MTR_3g030050 | 30.238 | 420 | 231 | 15 | 5 | 376 | 15 | 420 | 7.15e-35 | 133 |
| MS.gene012159.t1 | MTR_6g018390 | 29.722 | 360 | 189 | 13 | 5 | 340 | 4 | 323 | 5.76e-30 | 118 |
| MS.gene012159.t1 | MTR_3g058460 | 30.928 | 388 | 224 | 14 | 6 | 376 | 15 | 375 | 2.92e-29 | 117 |
| MS.gene012159.t1 | MTR_3g030360 | 29.620 | 368 | 204 | 14 | 3 | 351 | 2 | 333 | 4.14e-28 | 113 |
| MS.gene012159.t1 | MTR_3g029650 | 25.956 | 366 | 186 | 8 | 5 | 340 | 4 | 314 | 1.69e-27 | 114 |
| MS.gene012159.t1 | MTR_3g029650 | 25.405 | 370 | 216 | 15 | 4 | 342 | 370 | 710 | 7.86e-20 | 91.7 |
| MS.gene012159.t1 | MTR_1g007260 | 27.907 | 387 | 210 | 15 | 5 | 359 | 21 | 370 | 2.65e-26 | 108 |
| MS.gene012159.t1 | MTR_2g047180 | 27.692 | 390 | 237 | 13 | 5 | 376 | 3 | 365 | 5.60e-26 | 107 |
| MS.gene012159.t1 | MTR_4g125090 | 28.354 | 328 | 172 | 11 | 2 | 321 | 41 | 313 | 1.59e-25 | 105 |
| MS.gene012159.t1 | MTR_3g029620 | 31.680 | 363 | 193 | 16 | 5 | 345 | 2 | 331 | 1.77e-25 | 106 |
| MS.gene012159.t1 | MTR_6g027040 | 27.930 | 401 | 235 | 18 | 5 | 378 | 4 | 377 | 1.15e-24 | 104 |
| MS.gene012159.t1 | MTR_1g016570 | 29.070 | 344 | 187 | 12 | 6 | 342 | 21 | 314 | 7.07e-24 | 101 |
| MS.gene012159.t1 | MTR_6g027100 | 28.678 | 401 | 232 | 19 | 5 | 378 | 4 | 377 | 1.04e-23 | 102 |
| MS.gene012159.t1 | MTR_3g029920 | 29.275 | 386 | 204 | 16 | 4 | 361 | 7 | 351 | 1.25e-23 | 101 |
| MS.gene012159.t1 | MTR_6g018400 | 26.768 | 396 | 201 | 15 | 3 | 362 | 2 | 344 | 4.26e-23 | 99.8 |
| MS.gene012159.t1 | MTR_3g030440 | 28.818 | 347 | 208 | 12 | 1 | 342 | 1 | 313 | 4.99e-23 | 99.4 |
| MS.gene012159.t1 | MTR_3g030480 | 29.513 | 349 | 195 | 11 | 5 | 342 | 4 | 312 | 1.53e-22 | 97.8 |
| MS.gene012159.t1 | MTR_3g030380 | 26.724 | 348 | 210 | 10 | 4 | 342 | 3 | 314 | 2.37e-22 | 97.4 |
| MS.gene012159.t1 | MTR_3g029640 | 29.076 | 368 | 186 | 16 | 5 | 345 | 3 | 322 | 7.65e-22 | 95.9 |
| MS.gene012159.t1 | MTR_6g018360 | 28.611 | 360 | 207 | 16 | 5 | 342 | 4 | 335 | 2.53e-21 | 95.1 |
| MS.gene012159.t1 | MTR_1g049170 | 28.721 | 383 | 189 | 17 | 1 | 342 | 1 | 340 | 2.63e-21 | 94.7 |
| MS.gene012159.t1 | MTR_3g114990 | 26.799 | 403 | 229 | 17 | 5 | 374 | 22 | 391 | 7.46e-21 | 93.6 |
| MS.gene012159.t1 | MTR_3g030080 | 27.095 | 358 | 202 | 15 | 6 | 342 | 7 | 326 | 8.10e-21 | 93.2 |
| MS.gene012159.t1 | MTR_3g037710 | 36.905 | 168 | 98 | 3 | 180 | 342 | 55 | 219 | 1.20e-20 | 90.9 |
| MS.gene012159.t1 | MTR_3g029840 | 28.134 | 359 | 194 | 16 | 5 | 342 | 4 | 319 | 1.34e-20 | 92.4 |
| MS.gene012159.t1 | MTR_3g029950 | 27.824 | 363 | 197 | 16 | 5 | 345 | 4 | 323 | 1.49e-19 | 89.4 |
| MS.gene012159.t1 | MTR_3g114970 | 25.067 | 371 | 232 | 15 | 5 | 363 | 3 | 339 | 2.58e-19 | 88.6 |
| MS.gene012159.t1 | MTR_3g029670 | 24.390 | 369 | 221 | 9 | 5 | 343 | 4 | 344 | 6.56e-19 | 87.8 |
| MS.gene012159.t1 | MTR_3g030355 | 26.812 | 414 | 211 | 18 | 1 | 359 | 1 | 377 | 2.65e-18 | 85.9 |
| MS.gene012159.t1 | MTR_3g029750 | 23.545 | 378 | 229 | 8 | 5 | 351 | 4 | 352 | 3.94e-18 | 85.5 |
| MS.gene012159.t1 | MTR_3g091990 | 26.648 | 364 | 212 | 12 | 4 | 340 | 5 | 340 | 4.23e-18 | 85.5 |
| MS.gene012159.t1 | MTR_3g029820 | 28.426 | 394 | 162 | 17 | 4 | 370 | 7 | 307 | 2.34e-16 | 79.7 |
| MS.gene012159.t1 | MTR_3g047980 | 27.926 | 376 | 220 | 16 | 6 | 376 | 30 | 359 | 1.06e-15 | 78.2 |
| MS.gene012159.t1 | MTR_3g029900 | 28.846 | 312 | 159 | 14 | 68 | 368 | 177 | 436 | 5.62e-15 | 76.6 |
| MS.gene012159.t1 | MTR_6g018315 | 26.221 | 389 | 234 | 18 | 18 | 377 | 2 | 366 | 1.16e-14 | 75.1 |
| MS.gene012159.t1 | MTR_1g016540 | 35.664 | 143 | 74 | 5 | 207 | 342 | 2 | 133 | 1.14e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012159.t1 | AT2G17030 | 30.607 | 379 | 226 | 12 | 5 | 361 | 2 | 365 | 5.24e-40 | 146 |
| MS.gene012159.t1 | AT1G65770 | 30.252 | 357 | 219 | 11 | 5 | 354 | 2 | 335 | 5.58e-36 | 135 |
| MS.gene012159.t1 | AT4G35733 | 29.600 | 375 | 208 | 12 | 1 | 356 | 1 | 338 | 4.94e-35 | 132 |
| MS.gene012159.t1 | AT2G17036 | 27.614 | 373 | 239 | 11 | 5 | 361 | 2 | 359 | 1.75e-29 | 118 |
| MS.gene012159.t1 | AT2G17036 | 27.614 | 373 | 239 | 11 | 5 | 361 | 1 | 358 | 2.05e-29 | 117 |
| MS.gene012159.t1 | AT3G25750 | 28.760 | 379 | 205 | 14 | 3 | 356 | 2 | 340 | 3.22e-29 | 116 |
| MS.gene012159.t1 | AT1G44080 | 27.688 | 372 | 202 | 10 | 4 | 358 | 3 | 324 | 7.54e-29 | 115 |
| MS.gene012159.t1 | AT1G65740 | 28.767 | 365 | 226 | 12 | 5 | 354 | 2 | 347 | 4.56e-26 | 108 |
| MS.gene012159.t1 | AT3G25750 | 28.117 | 377 | 196 | 13 | 3 | 356 | 2 | 326 | 5.38e-26 | 107 |
| MS.gene012159.t1 | AT2G26160 | 28.531 | 354 | 204 | 12 | 3 | 342 | 2 | 320 | 1.34e-24 | 103 |
| MS.gene012159.t1 | AT1G65760 | 28.686 | 373 | 216 | 15 | 6 | 359 | 5 | 346 | 2.78e-23 | 100 |
| MS.gene012159.t1 | AT2G17690 | 27.423 | 423 | 218 | 14 | 6 | 359 | 3 | 405 | 3.68e-21 | 94.7 |
| MS.gene012159.t1 | AT2G16290 | 24.103 | 390 | 236 | 14 | 5 | 356 | 2 | 369 | 1.91e-18 | 86.7 |
| MS.gene012159.t1 | AT5G60060 | 28.307 | 378 | 219 | 19 | 7 | 361 | 11 | 359 | 2.56e-18 | 85.9 |
| MS.gene012159.t1 | AT1G64840 | 26.891 | 357 | 208 | 16 | 6 | 342 | 4 | 327 | 1.41e-16 | 80.9 |
| MS.gene012159.t1 | AT5G24040 | 23.280 | 378 | 205 | 16 | 5 | 346 | 2 | 330 | 1.71e-14 | 74.7 |
| MS.gene012159.t1 | AT1G57906 | 29.545 | 176 | 84 | 6 | 187 | 329 | 58 | 226 | 9.21e-12 | 64.7 |
Find 87 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAGCTTGTTGTATCTTTC+AGG | 0.173014 | 8.4:+30175082 | MS.gene012159:CDS |
| TTCAACAAGTTCCATATAAT+TGG | 0.175089 | 8.4:-30174942 | None:intergenic |
| AGGGAATTGCATCTACTTTA+AGG | 0.185868 | 8.4:+30175547 | MS.gene012159:CDS |
| TATGGAACTTGTTGAATTAT+AGG | 0.195427 | 8.4:+30174949 | MS.gene012159:CDS |
| AAAGGAAAAGAAGGAGAATT+TGG | 0.229744 | 8.4:-30174684 | None:intergenic |
| GTTTCTGCTAAAGAATTAAT+TGG | 0.230909 | 8.4:+30175518 | MS.gene012159:CDS |
| ATCTTGGACCATAGCTCTTT+GGG | 0.243195 | 8.4:-30174576 | None:intergenic |
| ATCTTTCAGGAAGGTAAATT+AGG | 0.270135 | 8.4:+30175095 | MS.gene012159:CDS |
| TATCTTGGACCATAGCTCTT+TGG | 0.291416 | 8.4:-30174577 | None:intergenic |
| ATGGAACTTGTTGAATTATA+GGG | 0.301160 | 8.4:+30174950 | MS.gene012159:CDS |
| TCACCTCCTTTGTGTGGGTT+TGG | 0.311829 | 8.4:+30175272 | MS.gene012159:CDS |
| TGAAATATGGAGACACAAAA+TGG | 0.318037 | 8.4:+30175123 | MS.gene012159:CDS |
| AAATTAGGATTCATGAAATA+TGG | 0.332072 | 8.4:+30175110 | MS.gene012159:CDS |
| ATCAAGATACTTCCCTATCT+TGG | 0.333662 | 8.4:-30174592 | None:intergenic |
| ACAACAGTTCTCCAATTATA+TGG | 0.333718 | 8.4:+30174931 | MS.gene012159:CDS |
| TTGATAGAGGTAATTGTATA+GGG | 0.347302 | 8.4:-30174733 | None:intergenic |
| CTCACAAAACTTGATGAGTA+TGG | 0.352709 | 8.4:-30175011 | None:intergenic |
| ACTCTCCTTGGATTAATGTT+GGG | 0.357299 | 8.4:+30175054 | MS.gene012159:CDS |
| ATTGATAGAGGTAATTGTAT+AGG | 0.365166 | 8.4:-30174734 | None:intergenic |
| CACTTTGATCAACCAACCTT+TGG | 0.369704 | 8.4:-30174829 | None:intergenic |
| AAAGAGCTATGGTCCAAGAT+AGG | 0.372622 | 8.4:+30174579 | MS.gene012159:CDS |
| ATAAATGGGGAACTATTTCA+TGG | 0.373052 | 8.4:+30175216 | MS.gene012159:CDS |
| CTTGCATTGACAAGACTTTG+TGG | 0.374534 | 8.4:+30175633 | MS.gene012159:CDS |
| AGGTAATTGTATAGGGAGAC+AGG | 0.376170 | 8.4:-30174726 | None:intergenic |
| ACATTAATCCAAGGAGAGTT+AGG | 0.379494 | 8.4:-30175050 | None:intergenic |
| AAAGAATTAATTGGATATCA+AGG | 0.389911 | 8.4:+30175527 | MS.gene012159:CDS |
| GATAATTTGCAGTCGGAGAT+AGG | 0.391629 | 8.4:-30174891 | None:intergenic |
| TTTATATGTTGGATGAAGAA+TGG | 0.404503 | 8.4:+30175426 | MS.gene012159:CDS |
| CCATGATTGGGACGCGGTTG+TGG | 0.410483 | 8.4:+30175391 | MS.gene012159:CDS |
| ATTTGGAAGAGAGGGAGGAA+TGG | 0.422238 | 8.4:-30174667 | None:intergenic |
| TGTCCTATCCTAACTCTCCT+TGG | 0.428803 | 8.4:+30175042 | MS.gene012159:CDS |
| TGATGATGTCATTGTATTCA+AGG | 0.430249 | 8.4:+30175175 | MS.gene012159:CDS |
| TTATATGTTGGATGAAGAAT+GGG | 0.432756 | 8.4:+30175427 | MS.gene012159:CDS |
| GATAAATAGTTGATTGATAG+AGG | 0.439760 | 8.4:-30174746 | None:intergenic |
| CTCTCCTTGGATTAATGTTG+GGG | 0.439895 | 8.4:+30175055 | MS.gene012159:CDS |
| AACATTTGGTGGAATCTTGT+GGG | 0.442266 | 8.4:+30175303 | MS.gene012159:CDS |
| GTTTGGAAACAAGAAACATT+TGG | 0.451619 | 8.4:+30175289 | MS.gene012159:CDS |
| AACTCTCCTTGGATTAATGT+TGG | 0.455668 | 8.4:+30175053 | MS.gene012159:CDS |
| GAAGGAGAATTTGGAAGAGA+GGG | 0.458355 | 8.4:-30174675 | None:intergenic |
| AAGTTGGAACTTGCATTAGT+TGG | 0.460926 | 8.4:-30174777 | None:intergenic |
| ATGTTGGAAGACGCCATGAT+TGG | 0.481058 | 8.4:+30175378 | MS.gene012159:CDS |
| AAACATTTGGTGGAATCTTG+TGG | 0.483180 | 8.4:+30175302 | MS.gene012159:CDS |
| CACGTTGGATGAGATGTTAG+AGG | 0.485509 | 8.4:+30175663 | MS.gene012159:CDS |
| AGAAGGAGAATTTGGAAGAG+AGG | 0.489920 | 8.4:-30174676 | None:intergenic |
| TGTTGGAAGACGCCATGATT+GGG | 0.493159 | 8.4:+30175379 | MS.gene012159:CDS |
| GGTATTTGTAAAGGAAAAGA+AGG | 0.498247 | 8.4:-30174693 | None:intergenic |
| ACAACTGCTGCTTAAAATGA+AGG | 0.500009 | 8.4:-30175486 | None:intergenic |
| CACCTTCTTATACCAAAGGT+TGG | 0.500177 | 8.4:+30174817 | MS.gene012159:CDS |
| CCACAACCGCGTCCCAATCA+TGG | 0.500362 | 8.4:-30175391 | None:intergenic |
| TAATGCTCGTATGTACAGCT+TGG | 0.504456 | 8.4:+30175577 | MS.gene012159:CDS |
| GGAGAATTTGGAAGAGAGGG+AGG | 0.512512 | 8.4:-30174672 | None:intergenic |
| AACCAACCTTTGGTATAAGA+AGG | 0.522904 | 8.4:-30174819 | None:intergenic |
| AACATGAGAGGGAACAATGT+TGG | 0.526890 | 8.4:+30175362 | MS.gene012159:CDS |
| TAAAGGTGGTAAATTGAAGT+TGG | 0.535440 | 8.4:-30174793 | None:intergenic |
| TTGGTATAAGAAGGTGCTAA+AGG | 0.538170 | 8.4:-30174810 | None:intergenic |
| AAGAGCTATGGTCCAAGATA+GGG | 0.546977 | 8.4:+30174580 | MS.gene012159:CDS |
| CTCATCTCCCCAAAGAGCTA+TGG | 0.548235 | 8.4:+30174568 | MS.gene012159:CDS |
| GGACTCAACAAACTCTTCAA+AGG | 0.551008 | 8.4:-30174870 | None:intergenic |
| GTGTTTCAAAGTTTATATGT+TGG | 0.555002 | 8.4:+30175415 | MS.gene012159:CDS |
| GGTAATTGTATAGGGAGACA+GGG | 0.555775 | 8.4:-30174725 | None:intergenic |
| AAGAATTAATTGGATATCAA+GGG | 0.561141 | 8.4:+30175528 | MS.gene012159:CDS |
| ATGGTGCGAGGTATTTGTAA+AGG | 0.569151 | 8.4:-30174702 | None:intergenic |
| TGGAAACAAGAAACATTTGG+TGG | 0.570071 | 8.4:+30175292 | MS.gene012159:CDS |
| TTAGCACCTTCTTATACCAA+AGG | 0.570602 | 8.4:+30174813 | MS.gene012159:CDS |
| CAAAGGTTGGTTGATCAAAG+TGG | 0.574189 | 8.4:+30174830 | MS.gene012159:CDS |
| CACAAAGTCTTGTCAATGCA+AGG | 0.575490 | 8.4:-30175632 | None:intergenic |
| GTTGTTGATAGATACTATGA+AGG | 0.579405 | 8.4:+30175335 | MS.gene012159:CDS |
| ATGAAGGTGAAAACATGAGA+GGG | 0.582267 | 8.4:+30175351 | MS.gene012159:CDS |
| GATGATGTCATTGTATTCAA+GGG | 0.582955 | 8.4:+30175176 | MS.gene012159:CDS |
| AGGATATGATAATTTGCAGT+CGG | 0.584721 | 8.4:-30174898 | None:intergenic |
| CTTTGTGGTCTCATTCACGT+TGG | 0.590681 | 8.4:+30175648 | MS.gene012159:CDS |
| GCTTGTTGTATCTTTCAGGA+AGG | 0.596136 | 8.4:+30175086 | MS.gene012159:CDS |
| GTATAAGAAGGTGCTAAAGG+TGG | 0.604135 | 8.4:-30174807 | None:intergenic |
| CAATCCCCAACATTAATCCA+AGG | 0.626001 | 8.4:-30175059 | None:intergenic |
| TCTTGGACCATAGCTCTTTG+GGG | 0.628895 | 8.4:-30174575 | None:intergenic |
| GAACTGTTGTTACTGTCAAG+AGG | 0.629902 | 8.4:-30174918 | None:intergenic |
| TATATGTTGGATGAAGAATG+GGG | 0.631666 | 8.4:+30175428 | MS.gene012159:CDS |
| ATTGTATAGGGAGACAGGGA+TGG | 0.639587 | 8.4:-30174721 | None:intergenic |
| AATCCAAGGAGAGTTAGGAT+AGG | 0.641071 | 8.4:-30175045 | None:intergenic |
| AGGGAGACAGGGATGGTGCG+AGG | 0.646104 | 8.4:-30174714 | None:intergenic |
| ATATGTTGGATGAAGAATGG+GGG | 0.646805 | 8.4:+30175429 | MS.gene012159:CDS |
| TATGAAGGTGAAAACATGAG+AGG | 0.647138 | 8.4:+30175350 | MS.gene012159:CDS |
| TTTCCAAACCCACACAAAGG+AGG | 0.652810 | 8.4:-30175275 | None:intergenic |
| AATGCAAGGATCAAAATCAA+CGG | 0.653913 | 8.4:-30175618 | None:intergenic |
| AGACACAAAATGGAAACTTG+TGG | 0.657681 | 8.4:+30175133 | MS.gene012159:CDS |
| TTGTTTCCAAACCCACACAA+AGG | 0.659541 | 8.4:-30175278 | None:intergenic |
| AAGACGCCATGATTGGGACG+CGG | 0.659617 | 8.4:+30175385 | MS.gene012159:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATTAATTGGATATCA+AGG | + | chr8.4:30175527-30175546 | MS.gene012159:CDS | 20.0% |
| !! | AAATTAGGATTCATGAAATA+TGG | + | chr8.4:30175110-30175129 | MS.gene012159:CDS | 20.0% |
| !! | AAGAATTAATTGGATATCAA+GGG | + | chr8.4:30175528-30175547 | MS.gene012159:CDS | 20.0% |
| !!! | AGTTTTATGTTACTGATAAA+TGG | + | chr8.4:30175201-30175220 | MS.gene012159:CDS | 20.0% |
| !!! | GTTTTATGTTACTGATAAAT+GGG | + | chr8.4:30175202-30175221 | MS.gene012159:CDS | 20.0% |
| !!! | TTTTATGTTACTGATAAATG+GGG | + | chr8.4:30175203-30175222 | MS.gene012159:CDS | 20.0% |
| ! | ATTGATAGAGGTAATTGTAT+AGG | - | chr8.4:30174737-30174756 | None:intergenic | 25.0% |
| ! | GATAAATAGTTGATTGATAG+AGG | - | chr8.4:30174749-30174768 | None:intergenic | 25.0% |
| ! | GTGTTTCAAAGTTTATATGT+TGG | + | chr8.4:30175415-30175434 | MS.gene012159:CDS | 25.0% |
| ! | GTTTCTGCTAAAGAATTAAT+TGG | + | chr8.4:30175518-30175537 | MS.gene012159:CDS | 25.0% |
| ! | TTATATGTTGGATGAAGAAT+GGG | + | chr8.4:30175427-30175446 | MS.gene012159:CDS | 25.0% |
| ! | TTCAACAAGTTCCATATAAT+TGG | - | chr8.4:30174945-30174964 | None:intergenic | 25.0% |
| ! | TTGATAGAGGTAATTGTATA+GGG | - | chr8.4:30174736-30174755 | None:intergenic | 25.0% |
| ! | TTTATATGTTGGATGAAGAA+TGG | + | chr8.4:30175426-30175445 | MS.gene012159:CDS | 25.0% |
| !!! | ATGGAACTTGTTGAATTATA+GGG | + | chr8.4:30174950-30174969 | MS.gene012159:CDS | 25.0% |
| !!! | TATGGAACTTGTTGAATTAT+AGG | + | chr8.4:30174949-30174968 | MS.gene012159:CDS | 25.0% |
| AAAGGAAAAGAAGGAGAATT+TGG | - | chr8.4:30174687-30174706 | None:intergenic | 30.0% | |
| AATGCAAGGATCAAAATCAA+CGG | - | chr8.4:30175621-30175640 | None:intergenic | 30.0% | |
| ACAACAGTTCTCCAATTATA+TGG | + | chr8.4:30174931-30174950 | MS.gene012159:CDS | 30.0% | |
| ATAAATGGGGAACTATTTCA+TGG | + | chr8.4:30175216-30175235 | MS.gene012159:CDS | 30.0% | |
| ATCTTTCAGGAAGGTAAATT+AGG | + | chr8.4:30175095-30175114 | MS.gene012159:CDS | 30.0% | |
| GATGATGTCATTGTATTCAA+GGG | + | chr8.4:30175176-30175195 | MS.gene012159:CDS | 30.0% | |
| GGTATTTGTAAAGGAAAAGA+AGG | - | chr8.4:30174696-30174715 | None:intergenic | 30.0% | |
| GTTTGGAAACAAGAAACATT+TGG | + | chr8.4:30175289-30175308 | MS.gene012159:CDS | 30.0% | |
| TAAAGGTGGTAAATTGAAGT+TGG | - | chr8.4:30174796-30174815 | None:intergenic | 30.0% | |
| TATATGTTGGATGAAGAATG+GGG | + | chr8.4:30175428-30175447 | MS.gene012159:CDS | 30.0% | |
| TGAAATATGGAGACACAAAA+TGG | + | chr8.4:30175123-30175142 | MS.gene012159:CDS | 30.0% | |
| ! | AGGATATGATAATTTGCAGT+CGG | - | chr8.4:30174901-30174920 | None:intergenic | 30.0% |
| ! | GTTGTTGATAGATACTATGA+AGG | + | chr8.4:30175335-30175354 | MS.gene012159:CDS | 30.0% |
| ! | TGATGATGTCATTGTATTCA+AGG | + | chr8.4:30175175-30175194 | MS.gene012159:CDS | 30.0% |
| AACCAACCTTTGGTATAAGA+AGG | - | chr8.4:30174822-30174841 | None:intergenic | 35.0% | |
| AACTCTCCTTGGATTAATGT+TGG | + | chr8.4:30175053-30175072 | MS.gene012159:CDS | 35.0% | |
| AAGTTGGAACTTGCATTAGT+TGG | - | chr8.4:30174780-30174799 | None:intergenic | 35.0% | |
| ACATTAATCCAAGGAGAGTT+AGG | - | chr8.4:30175053-30175072 | None:intergenic | 35.0% | |
| ACTCTCCTTGGATTAATGTT+GGG | + | chr8.4:30175054-30175073 | MS.gene012159:CDS | 35.0% | |
| AGACACAAAATGGAAACTTG+TGG | + | chr8.4:30175133-30175152 | MS.gene012159:CDS | 35.0% | |
| AGGGAATTGCATCTACTTTA+AGG | + | chr8.4:30175547-30175566 | MS.gene012159:CDS | 35.0% | |
| ATATGTTGGATGAAGAATGG+GGG | + | chr8.4:30175429-30175448 | MS.gene012159:CDS | 35.0% | |
| ATCAAGATACTTCCCTATCT+TGG | - | chr8.4:30174595-30174614 | None:intergenic | 35.0% | |
| ATGAAGGTGAAAACATGAGA+GGG | + | chr8.4:30175351-30175370 | MS.gene012159:CDS | 35.0% | |
| CTCACAAAACTTGATGAGTA+TGG | - | chr8.4:30175014-30175033 | None:intergenic | 35.0% | |
| TATGAAGGTGAAAACATGAG+AGG | + | chr8.4:30175350-30175369 | MS.gene012159:CDS | 35.0% | |
| TGGAAACAAGAAACATTTGG+TGG | + | chr8.4:30175292-30175311 | MS.gene012159:CDS | 35.0% | |
| TTAGCACCTTCTTATACCAA+AGG | + | chr8.4:30174813-30174832 | MS.gene012159:CDS | 35.0% | |
| ! | AAACATTTGGTGGAATCTTG+TGG | + | chr8.4:30175302-30175321 | MS.gene012159:CDS | 35.0% |
| ! | AACATTTGGTGGAATCTTGT+GGG | + | chr8.4:30175303-30175322 | MS.gene012159:CDS | 35.0% |
| ! | AATTTTCACCTCCTTTGTGT+GGG | + | chr8.4:30175267-30175286 | MS.gene012159:CDS | 35.0% |
| ! | ACAACTGCTGCTTAAAATGA+AGG | - | chr8.4:30175489-30175508 | None:intergenic | 35.0% |
| !! | TGTAGCTTGTTGTATCTTTC+AGG | + | chr8.4:30175082-30175101 | MS.gene012159:CDS | 35.0% |
| !! | TTGGTATAAGAAGGTGCTAA+AGG | - | chr8.4:30174813-30174832 | None:intergenic | 35.0% |
| AAAGAGCTATGGTCCAAGAT+AGG | + | chr8.4:30174579-30174598 | MS.gene012159:CDS | 40.0% | |
| AACATGAGAGGGAACAATGT+TGG | + | chr8.4:30175362-30175381 | MS.gene012159:CDS | 40.0% | |
| AAGAGCTATGGTCCAAGATA+GGG | + | chr8.4:30174580-30174599 | MS.gene012159:CDS | 40.0% | |
| AATCCAAGGAGAGTTAGGAT+AGG | - | chr8.4:30175048-30175067 | None:intergenic | 40.0% | |
| AGAAGGAGAATTTGGAAGAG+AGG | - | chr8.4:30174679-30174698 | None:intergenic | 40.0% | |
| AGGTAATTGTATAGGGAGAC+AGG | - | chr8.4:30174729-30174748 | None:intergenic | 40.0% | |
| ATCTTGGACCATAGCTCTTT+GGG | - | chr8.4:30174579-30174598 | None:intergenic | 40.0% | |
| CAATCCCCAACATTAATCCA+AGG | - | chr8.4:30175062-30175081 | None:intergenic | 40.0% | |
| CACCTTCTTATACCAAAGGT+TGG | + | chr8.4:30174817-30174836 | MS.gene012159:CDS | 40.0% | |
| CACTTTGATCAACCAACCTT+TGG | - | chr8.4:30174832-30174851 | None:intergenic | 40.0% | |
| CTCTCCTTGGATTAATGTTG+GGG | + | chr8.4:30175055-30175074 | MS.gene012159:CDS | 40.0% | |
| GAACTGTTGTTACTGTCAAG+AGG | - | chr8.4:30174921-30174940 | None:intergenic | 40.0% | |
| GAAGGAGAATTTGGAAGAGA+GGG | - | chr8.4:30174678-30174697 | None:intergenic | 40.0% | |
| GGACTCAACAAACTCTTCAA+AGG | - | chr8.4:30174873-30174892 | None:intergenic | 40.0% | |
| GGTAATTGTATAGGGAGACA+GGG | - | chr8.4:30174728-30174747 | None:intergenic | 40.0% | |
| TAATGCTCGTATGTACAGCT+TGG | + | chr8.4:30175577-30175596 | MS.gene012159:CDS | 40.0% | |
| TATCTTGGACCATAGCTCTT+TGG | - | chr8.4:30174580-30174599 | None:intergenic | 40.0% | |
| TTGTTTCCAAACCCACACAA+AGG | - | chr8.4:30175281-30175300 | None:intergenic | 40.0% | |
| ! | ATGGTGCGAGGTATTTGTAA+AGG | - | chr8.4:30174705-30174724 | None:intergenic | 40.0% |
| ! | CAAAGGTTGGTTGATCAAAG+TGG | + | chr8.4:30174830-30174849 | MS.gene012159:CDS | 40.0% |
| ! | CAATTTTCACCTCCTTTGTG+TGG | + | chr8.4:30175266-30175285 | MS.gene012159:CDS | 40.0% |
| ! | CACAAAGTCTTGTCAATGCA+AGG | - | chr8.4:30175635-30175654 | None:intergenic | 40.0% |
| ! | CTTGCATTGACAAGACTTTG+TGG | + | chr8.4:30175633-30175652 | MS.gene012159:CDS | 40.0% |
| ! | GATAATTTGCAGTCGGAGAT+AGG | - | chr8.4:30174894-30174913 | None:intergenic | 40.0% |
| !! | GCTTGTTGTATCTTTCAGGA+AGG | + | chr8.4:30175086-30175105 | MS.gene012159:CDS | 40.0% |
| !! | GTATAAGAAGGTGCTAAAGG+TGG | - | chr8.4:30174810-30174829 | None:intergenic | 40.0% |
| ATGTTGGAAGACGCCATGAT+TGG | + | chr8.4:30175378-30175397 | MS.gene012159:CDS | 45.0% | |
| ATTGTATAGGGAGACAGGGA+TGG | - | chr8.4:30174724-30174743 | None:intergenic | 45.0% | |
| ATTTGGAAGAGAGGGAGGAA+TGG | - | chr8.4:30174670-30174689 | None:intergenic | 45.0% | |
| CTTTGTGGTCTCATTCACGT+TGG | + | chr8.4:30175648-30175667 | MS.gene012159:CDS | 45.0% | |
| GGATGAAGAATGGGGGAAAT+GGG | + | chr8.4:30175436-30175455 | MS.gene012159:CDS | 45.0% | |
| TCTTGGACCATAGCTCTTTG+GGG | - | chr8.4:30174578-30174597 | None:intergenic | 45.0% | |
| TGGATGAAGAATGGGGGAAA+TGG | + | chr8.4:30175435-30175454 | MS.gene012159:CDS | 45.0% | |
| TGTCCTATCCTAACTCTCCT+TGG | + | chr8.4:30175042-30175061 | MS.gene012159:CDS | 45.0% | |
| TGTTGGAAGACGCCATGATT+GGG | + | chr8.4:30175379-30175398 | MS.gene012159:CDS | 45.0% | |
| TTTCCAAACCCACACAAAGG+AGG | - | chr8.4:30175278-30175297 | None:intergenic | 45.0% | |
| CTCATCTCCCCAAAGAGCTA+TGG | + | chr8.4:30174568-30174587 | MS.gene012159:CDS | 50.0% | |
| GGAGAATTTGGAAGAGAGGG+AGG | - | chr8.4:30174675-30174694 | None:intergenic | 50.0% | |
| TCACCTCCTTTGTGTGGGTT+TGG | + | chr8.4:30175272-30175291 | MS.gene012159:CDS | 50.0% | |
| AAGACGCCATGATTGGGACG+CGG | + | chr8.4:30175385-30175404 | MS.gene012159:CDS | 55.0% | |
| CCACAACCGCGTCCCAATCA+TGG | - | chr8.4:30175394-30175413 | None:intergenic | 60.0% | |
| CCATGATTGGGACGCGGTTG+TGG | + | chr8.4:30175391-30175410 | MS.gene012159:CDS | 60.0% | |
| AGGGAGACAGGGATGGTGCG+AGG | - | chr8.4:30174717-30174736 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 30174546 | 30175682 | 30174546 | ID=MS.gene012159 |
| chr8.4 | mRNA | 30174546 | 30175682 | 30174546 | ID=MS.gene012159.t1;Parent=MS.gene012159 |
| chr8.4 | exon | 30174546 | 30175682 | 30174546 | ID=MS.gene012159.t1.exon1;Parent=MS.gene012159.t1 |
| chr8.4 | CDS | 30174546 | 30175682 | 30174546 | ID=cds.MS.gene012159.t1;Parent=MS.gene012159.t1 |
| Gene Sequence |
| Protein sequence |
