Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28903.t1 | XP_003629214.1 | 90.7 | 378 | 34 | 1 | 1 | 378 | 1 | 377 | 4.50E-207 | 730.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28903.t1 | Q9SHX9 | 30.7 | 358 | 212 | 10 | 6 | 353 | 3 | 334 | 8.7e-34 | 146.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28903.t1 | G7LA99 | 90.7 | 378 | 34 | 1 | 1 | 378 | 1 | 377 | 3.2e-207 | 730.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene44190 | MS.gene28903 | PPI |
| MS.gene28903 | MS.gene017581 | PPI |
| MS.gene97013 | MS.gene28903 | PPI |
| MS.gene64423 | MS.gene28903 | PPI |
| MS.gene28903 | MS.gene023531 | PPI |
| MS.gene012159 | MS.gene28903 | PPI |
| MS.gene28903 | MS.gene018718 | PPI |
| MS.gene28903 | MS.gene049886 | PPI |
| MS.gene28903 | MS.gene97013 | PPI |
| MS.gene037197 | MS.gene28903 | PPI |
| MS.gene30094 | MS.gene28903 | PPI |
| MS.gene91760 | MS.gene28903 | PPI |
| MS.gene28903 | MS.gene012160 | PPI |
| MS.gene28903 | MS.gene017582 | PPI |
| MS.gene28903 | MS.gene39445 | PPI |
| MS.gene28903 | MS.gene064059 | PPI |
| MS.gene28903 | MS.gene08770 | PPI |
| MS.gene28903 | MS.gene025209 | PPI |
| MS.gene23497 | MS.gene28903 | PPI |
| MS.gene28903 | MS.gene28904 | PPI |
| MS.gene23495 | MS.gene28903 | PPI |
| MS.gene28903 | MS.gene66307 | PPI |
| MS.gene28903 | MS.gene44191 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28903.t1 | MTR_8g074640 | 90.741 | 378 | 34 | 1 | 1 | 378 | 1 | 377 | 0.0 | 700 |
| MS.gene28903.t1 | MTR_8g074610 | 75.789 | 380 | 88 | 4 | 1 | 378 | 20 | 397 | 0.0 | 586 |
| MS.gene28903.t1 | MTR_2g041510 | 75.328 | 381 | 82 | 5 | 1 | 378 | 21 | 392 | 0.0 | 554 |
| MS.gene28903.t1 | MTR_2g041510 | 75.328 | 381 | 82 | 5 | 1 | 378 | 1 | 372 | 0.0 | 553 |
| MS.gene28903.t1 | MTR_5g079830 | 35.127 | 353 | 182 | 12 | 1 | 340 | 1 | 319 | 2.33e-50 | 174 |
| MS.gene28903.t1 | MTR_6g092570 | 34.400 | 375 | 199 | 11 | 2 | 364 | 4 | 343 | 2.63e-49 | 170 |
| MS.gene28903.t1 | MTR_8g085760 | 34.973 | 366 | 187 | 12 | 2 | 340 | 9 | 350 | 5.38e-49 | 170 |
| MS.gene28903.t1 | MTR_7g096720 | 33.146 | 356 | 193 | 11 | 8 | 340 | 15 | 348 | 2.97e-44 | 157 |
| MS.gene28903.t1 | MTR_4g088925 | 32.853 | 347 | 180 | 13 | 14 | 340 | 34 | 347 | 7.78e-39 | 143 |
| MS.gene28903.t1 | MTR_3g030050 | 31.132 | 424 | 222 | 15 | 5 | 376 | 15 | 420 | 5.14e-36 | 136 |
| MS.gene28903.t1 | MTR_1g026850 | 28.928 | 401 | 222 | 12 | 1 | 376 | 1 | 363 | 4.26e-35 | 132 |
| MS.gene28903.t1 | MTR_6g018390 | 28.947 | 380 | 204 | 14 | 5 | 360 | 4 | 341 | 4.77e-29 | 116 |
| MS.gene28903.t1 | MTR_3g058460 | 32.659 | 346 | 197 | 13 | 6 | 342 | 15 | 333 | 7.95e-29 | 115 |
| MS.gene28903.t1 | MTR_3g029650 | 27.887 | 355 | 193 | 10 | 5 | 340 | 4 | 314 | 1.69e-27 | 114 |
| MS.gene28903.t1 | MTR_3g029650 | 25.414 | 362 | 226 | 11 | 4 | 342 | 370 | 710 | 2.76e-19 | 90.1 |
| MS.gene28903.t1 | MTR_3g030360 | 29.526 | 359 | 200 | 13 | 3 | 342 | 2 | 326 | 1.86e-27 | 111 |
| MS.gene28903.t1 | MTR_4g125090 | 28.354 | 328 | 172 | 11 | 2 | 321 | 41 | 313 | 4.58e-26 | 107 |
| MS.gene28903.t1 | MTR_2g047180 | 30.028 | 353 | 206 | 14 | 5 | 342 | 3 | 329 | 6.37e-26 | 107 |
| MS.gene28903.t1 | MTR_1g007260 | 28.493 | 365 | 197 | 14 | 5 | 342 | 21 | 348 | 8.93e-26 | 107 |
| MS.gene28903.t1 | MTR_3g029620 | 31.405 | 363 | 194 | 16 | 5 | 345 | 2 | 331 | 1.79e-24 | 103 |
| MS.gene28903.t1 | MTR_6g027040 | 27.930 | 401 | 235 | 18 | 5 | 378 | 4 | 377 | 3.70e-24 | 102 |
| MS.gene28903.t1 | MTR_6g027100 | 29.064 | 406 | 224 | 21 | 5 | 378 | 4 | 377 | 1.21e-23 | 102 |
| MS.gene28903.t1 | MTR_3g029920 | 29.016 | 386 | 205 | 16 | 4 | 361 | 7 | 351 | 6.51e-23 | 99.4 |
| MS.gene28903.t1 | MTR_3g030380 | 27.586 | 348 | 207 | 11 | 4 | 342 | 3 | 314 | 8.93e-23 | 98.6 |
| MS.gene28903.t1 | MTR_6g018400 | 27.525 | 396 | 198 | 16 | 3 | 362 | 2 | 344 | 9.85e-23 | 98.6 |
| MS.gene28903.t1 | MTR_3g030480 | 29.971 | 347 | 196 | 10 | 5 | 342 | 4 | 312 | 1.06e-22 | 98.2 |
| MS.gene28903.t1 | MTR_3g030440 | 28.242 | 347 | 210 | 11 | 1 | 342 | 1 | 313 | 1.33e-22 | 98.2 |
| MS.gene28903.t1 | MTR_1g016570 | 28.779 | 344 | 188 | 12 | 6 | 342 | 21 | 314 | 2.12e-22 | 97.4 |
| MS.gene28903.t1 | MTR_6g018360 | 28.532 | 361 | 206 | 16 | 5 | 342 | 4 | 335 | 5.76e-22 | 97.1 |
| MS.gene28903.t1 | MTR_1g049170 | 27.851 | 377 | 200 | 14 | 1 | 342 | 1 | 340 | 1.01e-21 | 95.9 |
| MS.gene28903.t1 | MTR_3g029640 | 28.338 | 367 | 190 | 14 | 5 | 345 | 3 | 322 | 1.58e-21 | 95.1 |
| MS.gene28903.t1 | MTR_3g030080 | 27.374 | 358 | 201 | 15 | 6 | 342 | 7 | 326 | 2.03e-20 | 92.0 |
| MS.gene28903.t1 | MTR_3g114990 | 26.829 | 369 | 211 | 16 | 5 | 345 | 22 | 359 | 2.40e-20 | 92.0 |
| MS.gene28903.t1 | MTR_3g037710 | 36.310 | 168 | 99 | 3 | 180 | 342 | 55 | 219 | 5.64e-20 | 89.4 |
| MS.gene28903.t1 | MTR_3g029840 | 27.855 | 359 | 195 | 16 | 5 | 342 | 4 | 319 | 4.86e-19 | 87.8 |
| MS.gene28903.t1 | MTR_3g029750 | 25.798 | 376 | 223 | 11 | 5 | 351 | 4 | 352 | 6.38e-19 | 87.8 |
| MS.gene28903.t1 | MTR_3g114970 | 25.337 | 371 | 231 | 16 | 5 | 363 | 3 | 339 | 6.72e-19 | 87.4 |
| MS.gene28903.t1 | MTR_3g029670 | 24.661 | 369 | 220 | 9 | 5 | 343 | 4 | 344 | 7.56e-19 | 87.4 |
| MS.gene28903.t1 | MTR_3g029950 | 27.248 | 367 | 196 | 16 | 5 | 346 | 4 | 324 | 1.07e-18 | 87.0 |
| MS.gene28903.t1 | MTR_3g030355 | 26.730 | 419 | 213 | 18 | 1 | 363 | 1 | 381 | 1.20e-18 | 87.0 |
| MS.gene28903.t1 | MTR_3g091990 | 26.648 | 364 | 212 | 12 | 4 | 340 | 5 | 340 | 1.73e-17 | 84.0 |
| MS.gene28903.t1 | MTR_3g029820 | 28.680 | 394 | 161 | 17 | 4 | 370 | 7 | 307 | 7.90e-16 | 78.2 |
| MS.gene28903.t1 | MTR_6g018370 | 27.445 | 317 | 161 | 11 | 4 | 318 | 2 | 251 | 1.13e-15 | 76.6 |
| MS.gene28903.t1 | MTR_3g047980 | 27.926 | 376 | 220 | 16 | 6 | 376 | 30 | 359 | 7.11e-15 | 75.5 |
| MS.gene28903.t1 | MTR_6g018315 | 25.823 | 395 | 228 | 17 | 18 | 377 | 2 | 366 | 9.81e-15 | 75.5 |
| MS.gene28903.t1 | MTR_3g029900 | 29.252 | 294 | 153 | 13 | 68 | 349 | 177 | 427 | 1.07e-13 | 72.8 |
| MS.gene28903.t1 | MTR_1g016540 | 34.965 | 143 | 75 | 4 | 207 | 342 | 2 | 133 | 3.54e-12 | 65.1 |
| MS.gene28903.t1 | MTR_1g007250 | 32.168 | 143 | 81 | 4 | 209 | 348 | 4 | 133 | 7.66e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28903.t1 | AT2G17030 | 30.871 | 379 | 225 | 13 | 5 | 361 | 2 | 365 | 2.62e-39 | 145 |
| MS.gene28903.t1 | AT1G65770 | 30.495 | 364 | 209 | 11 | 5 | 354 | 2 | 335 | 1.85e-38 | 141 |
| MS.gene28903.t1 | AT4G35733 | 29.600 | 375 | 208 | 12 | 1 | 356 | 1 | 338 | 1.34e-34 | 131 |
| MS.gene28903.t1 | AT2G17036 | 27.926 | 376 | 234 | 12 | 5 | 361 | 2 | 359 | 3.65e-30 | 120 |
| MS.gene28903.t1 | AT2G17036 | 27.926 | 376 | 234 | 12 | 5 | 361 | 1 | 358 | 3.67e-30 | 120 |
| MS.gene28903.t1 | AT3G25750 | 29.288 | 379 | 203 | 15 | 3 | 356 | 2 | 340 | 8.09e-30 | 118 |
| MS.gene28903.t1 | AT1G44080 | 28.723 | 376 | 193 | 12 | 4 | 358 | 3 | 324 | 1.30e-28 | 114 |
| MS.gene28903.t1 | AT1G65740 | 29.041 | 365 | 225 | 12 | 5 | 354 | 2 | 347 | 2.66e-27 | 111 |
| MS.gene28903.t1 | AT3G25750 | 28.647 | 377 | 194 | 14 | 3 | 356 | 2 | 326 | 1.27e-26 | 109 |
| MS.gene28903.t1 | AT2G26160 | 28.969 | 359 | 204 | 13 | 3 | 347 | 2 | 323 | 5.75e-25 | 104 |
| MS.gene28903.t1 | AT1G65760 | 28.686 | 373 | 216 | 15 | 6 | 359 | 5 | 346 | 5.04e-24 | 102 |
| MS.gene28903.t1 | AT2G17690 | 27.208 | 419 | 224 | 13 | 6 | 359 | 3 | 405 | 5.79e-23 | 100 |
| MS.gene28903.t1 | AT5G60060 | 28.412 | 359 | 208 | 18 | 7 | 345 | 11 | 340 | 2.12e-17 | 83.2 |
| MS.gene28903.t1 | AT2G16290 | 25.272 | 368 | 227 | 14 | 5 | 340 | 2 | 353 | 3.64e-17 | 82.8 |
| MS.gene28903.t1 | AT1G64840 | 26.611 | 357 | 209 | 16 | 6 | 342 | 4 | 327 | 8.08e-16 | 78.6 |
| MS.gene28903.t1 | AT5G24040 | 23.747 | 379 | 194 | 17 | 5 | 342 | 2 | 326 | 3.44e-13 | 70.5 |
| MS.gene28903.t1 | AT1G57906 | 32.386 | 176 | 79 | 7 | 187 | 329 | 58 | 226 | 5.36e-13 | 68.2 |
Find 86 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAGCTTGTTGTATCTTTC+AGG | 0.173014 | 8.2:+30146788 | MS.gene28903:CDS |
| TTCAACAAGTTCCATATAAT+TGG | 0.175089 | 8.2:-30146648 | None:intergenic |
| AGGGAATTGCATCTACTTTA+AGG | 0.185868 | 8.2:+30147253 | MS.gene28903:CDS |
| TATGGAACTTGTTGAATTAT+AGG | 0.195427 | 8.2:+30146655 | MS.gene28903:CDS |
| GTTTCAGCTAAAGAATTAAT+TGG | 0.219543 | 8.2:+30147224 | MS.gene28903:CDS |
| AAAGGAAAAGAAGGAGAATT+TGG | 0.229744 | 8.2:-30146390 | None:intergenic |
| ATCTTGGACCATAGCTCTTT+GGG | 0.243195 | 8.2:-30146282 | None:intergenic |
| ATCTTTCAGGAAGGTAAATT+AGG | 0.270135 | 8.2:+30146801 | MS.gene28903:CDS |
| TATCTTGGACCATAGCTCTT+TGG | 0.291416 | 8.2:-30146283 | None:intergenic |
| ATGGAACTTGTTGAATTATA+GGG | 0.301160 | 8.2:+30146656 | MS.gene28903:CDS |
| TCACCTCCTTTGTGTGGGTT+TGG | 0.311829 | 8.2:+30146978 | MS.gene28903:CDS |
| ATTGATGGAGGTAATTGTAT+AGG | 0.314786 | 8.2:-30146440 | None:intergenic |
| TGAAATATGGAGACACAAAA+TGG | 0.318037 | 8.2:+30146829 | MS.gene28903:CDS |
| AAATTAGGATTCATGAAATA+TGG | 0.332072 | 8.2:+30146816 | MS.gene28903:CDS |
| ATCAAGATACTTCCCTATCT+TGG | 0.333662 | 8.2:-30146298 | None:intergenic |
| ACAACAGTTCTCCAATTATA+TGG | 0.333718 | 8.2:+30146637 | MS.gene28903:CDS |
| CTCACAAAACTTGATGAGTA+TGG | 0.352709 | 8.2:-30146717 | None:intergenic |
| ACTCTCCTTGGATTAATGTT+GGG | 0.357299 | 8.2:+30146760 | MS.gene28903:CDS |
| CACTTTGATCAACCAACCTT+TGG | 0.369704 | 8.2:-30146535 | None:intergenic |
| GATAATTTGCAGTCGGAGAT+AGG | 0.369983 | 8.2:-30146597 | None:intergenic |
| AAAGAGCTATGGTCCAAGAT+AGG | 0.372622 | 8.2:+30146285 | MS.gene28903:CDS |
| ATAAATGGGGAACTATTTCA+TGG | 0.373052 | 8.2:+30146922 | MS.gene28903:CDS |
| ACATTAATCCAAGGAGAGTT+AGG | 0.379494 | 8.2:-30146756 | None:intergenic |
| TCCAACATTGTTCCCTCTCA+TGG | 0.381515 | 8.2:-30147069 | None:intergenic |
| TTGATGGAGGTAATTGTATA+GGG | 0.382048 | 8.2:-30146439 | None:intergenic |
| AAAGAATTAATTGGATATCA+AGG | 0.389911 | 8.2:+30147233 | MS.gene28903:CDS |
| AGGTAATTGTATAGGGAGAC+AGG | 0.390095 | 8.2:-30146432 | None:intergenic |
| CTTGCATTGACAAGACTTTG+TGG | 0.395522 | 8.2:+30147339 | MS.gene28903:CDS |
| TTTATATGTTGGATGAAGAA+TGG | 0.404503 | 8.2:+30147132 | MS.gene28903:CDS |
| TAAGATAAATAGTTGATTGA+TGG | 0.404685 | 8.2:-30146455 | None:intergenic |
| ATTTGGAAGAGAGGGAGGAA+TGG | 0.422238 | 8.2:-30146373 | None:intergenic |
| TGATGATGTCATTGTATTCA+AGG | 0.430249 | 8.2:+30146881 | MS.gene28903:CDS |
| TTATATGTTGGATGAAGAAT+GGG | 0.432756 | 8.2:+30147133 | MS.gene28903:CDS |
| TGTCATATCCTAACTCTCCT+TGG | 0.439352 | 8.2:+30146748 | MS.gene28903:CDS |
| CTCTCCTTGGATTAATGTTG+GGG | 0.439895 | 8.2:+30146761 | MS.gene28903:CDS |
| AACATTTGGTGGAATCTTGT+GGG | 0.442266 | 8.2:+30147009 | MS.gene28903:CDS |
| GTTTGGAAACAAGAAACATT+TGG | 0.451619 | 8.2:+30146995 | MS.gene28903:CDS |
| AACTCTCCTTGGATTAATGT+TGG | 0.455668 | 8.2:+30146759 | MS.gene28903:CDS |
| GAAGGAGAATTTGGAAGAGA+GGG | 0.458355 | 8.2:-30146381 | None:intergenic |
| CCATGATCAGGACGCGGTTG+TGG | 0.460153 | 8.2:+30147097 | MS.gene28903:CDS |
| AAGTTGGAACTTGCATTAGT+TGG | 0.460926 | 8.2:-30146483 | None:intergenic |
| TGTTGGAAGACGCCATGATC+AGG | 0.461480 | 8.2:+30147085 | MS.gene28903:CDS |
| CCACAACCGCGTCCTGATCA+TGG | 0.476137 | 8.2:-30147097 | None:intergenic |
| AAACATTTGGTGGAATCTTG+TGG | 0.483180 | 8.2:+30147008 | MS.gene28903:CDS |
| AGAAGGAGAATTTGGAAGAG+AGG | 0.489920 | 8.2:-30146382 | None:intergenic |
| GGTATTTGTAAAGGAAAAGA+AGG | 0.498247 | 8.2:-30146399 | None:intergenic |
| ACAACTGCTGCTTAAAATGA+AGG | 0.500009 | 8.2:-30147192 | None:intergenic |
| CACCTTCTTATACCAAAGGT+TGG | 0.500177 | 8.2:+30146523 | MS.gene28903:CDS |
| GGAGAATTTGGAAGAGAGGG+AGG | 0.512512 | 8.2:-30146378 | None:intergenic |
| TAATGTTCGTATGTACAGCT+TGG | 0.516301 | 8.2:+30147283 | MS.gene28903:CDS |
| AACCAACCTTTGGTATAAGA+AGG | 0.522904 | 8.2:-30146525 | None:intergenic |
| TAAAGGTGGTAAATTGAAGT+TGG | 0.535440 | 8.2:-30146499 | None:intergenic |
| ACCATGAGAGGGAACAATGT+TGG | 0.537539 | 8.2:+30147068 | MS.gene28903:CDS |
| TTGGTATAAGAAGGTGCTAA+AGG | 0.538170 | 8.2:-30146516 | None:intergenic |
| GATAAATAGTTGATTGATGG+AGG | 0.541657 | 8.2:-30146452 | None:intergenic |
| AAGAGCTATGGTCCAAGATA+GGG | 0.546977 | 8.2:+30146286 | MS.gene28903:CDS |
| CTCATCTCCCCAAAGAGCTA+TGG | 0.548235 | 8.2:+30146274 | MS.gene28903:CDS |
| GTGTTTCAAAGTTTATATGT+TGG | 0.555002 | 8.2:+30147121 | MS.gene28903:CDS |
| AAGAATTAATTGGATATCAA+GGG | 0.561141 | 8.2:+30147234 | MS.gene28903:CDS |
| CTTTGTGGTTTCATTCACGT+TGG | 0.568595 | 8.2:+30147354 | MS.gene28903:CDS |
| CACAAAGTCTTGTCAATGCA+AGG | 0.568670 | 8.2:-30147338 | None:intergenic |
| ATGGTGCGAGGTATTTGTAA+AGG | 0.569151 | 8.2:-30146408 | None:intergenic |
| TGGAAACAAGAAACATTTGG+TGG | 0.570071 | 8.2:+30146998 | MS.gene28903:CDS |
| TTAGCACCTTCTTATACCAA+AGG | 0.570602 | 8.2:+30146519 | MS.gene28903:CDS |
| CAAAGGTTGGTTGATCAAAG+TGG | 0.574189 | 8.2:+30146536 | MS.gene28903:CDS |
| GGATTCAACAAACTCTTCAA+AGG | 0.574852 | 8.2:-30146576 | None:intergenic |
| GTTGTTGATAGATACTATGA+AGG | 0.579405 | 8.2:+30147041 | MS.gene28903:CDS |
| GATGATGTCATTGTATTCAA+GGG | 0.582955 | 8.2:+30146882 | MS.gene28903:CDS |
| AGGATATGATAATTTGCAGT+CGG | 0.584721 | 8.2:-30146604 | None:intergenic |
| GCTTGTTGTATCTTTCAGGA+AGG | 0.596136 | 8.2:+30146792 | MS.gene28903:CDS |
| GTATAAGAAGGTGCTAAAGG+TGG | 0.604135 | 8.2:-30146513 | None:intergenic |
| ATGAAGGTGAAACCATGAGA+GGG | 0.625558 | 8.2:+30147057 | MS.gene28903:CDS |
| CAATCCCCAACATTAATCCA+AGG | 0.626001 | 8.2:-30146765 | None:intergenic |
| TCTTGGACCATAGCTCTTTG+GGG | 0.628895 | 8.2:-30146281 | None:intergenic |
| GAACTGTTGTTACTGTCAAG+AGG | 0.629902 | 8.2:-30146624 | None:intergenic |
| TATATGTTGGATGAAGAATG+GGG | 0.631666 | 8.2:+30147134 | MS.gene28903:CDS |
| ATTGTATAGGGAGACAGGGA+TGG | 0.639587 | 8.2:-30146427 | None:intergenic |
| AGGGAGACAGGGATGGTGCG+AGG | 0.646104 | 8.2:-30146420 | None:intergenic |
| ATATGTTGGATGAAGAATGG+GGG | 0.646805 | 8.2:+30147135 | MS.gene28903:CDS |
| TTTCCAAACCCACACAAAGG+AGG | 0.652810 | 8.2:-30146981 | None:intergenic |
| AATGCAAGGATCAAAATCAA+CGG | 0.653913 | 8.2:-30147324 | None:intergenic |
| AGACACAAAATGGAAACTTG+TGG | 0.657681 | 8.2:+30146839 | MS.gene28903:CDS |
| GGTAATTGTATAGGGAGACA+GGG | 0.658699 | 8.2:-30146431 | None:intergenic |
| TTGTTTCCAAACCCACACAA+AGG | 0.659541 | 8.2:-30146984 | None:intergenic |
| TATGAAGGTGAAACCATGAG+AGG | 0.666026 | 8.2:+30147056 | MS.gene28903:CDS |
| AAGACGCCATGATCAGGACG+CGG | 0.698931 | 8.2:+30147091 | MS.gene28903:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATTAATTGGATATCA+AGG | + | chr8.2:30147233-30147252 | MS.gene28903:CDS | 20.0% |
| !! | AAATTAGGATTCATGAAATA+TGG | + | chr8.2:30146816-30146835 | MS.gene28903:CDS | 20.0% |
| !! | AAGAATTAATTGGATATCAA+GGG | + | chr8.2:30147234-30147253 | MS.gene28903:CDS | 20.0% |
| !! | TAAGATAAATAGTTGATTGA+TGG | - | chr8.2:30146458-30146477 | None:intergenic | 20.0% |
| !!! | AGTTTTATGTTACTGATAAA+TGG | + | chr8.2:30146907-30146926 | MS.gene28903:CDS | 20.0% |
| !!! | GTTTTATGTTACTGATAAAT+GGG | + | chr8.2:30146908-30146927 | MS.gene28903:CDS | 20.0% |
| !!! | TTTTATGTTACTGATAAATG+GGG | + | chr8.2:30146909-30146928 | MS.gene28903:CDS | 20.0% |
| ! | GTGTTTCAAAGTTTATATGT+TGG | + | chr8.2:30147121-30147140 | MS.gene28903:CDS | 25.0% |
| ! | GTTTCAGCTAAAGAATTAAT+TGG | + | chr8.2:30147224-30147243 | MS.gene28903:CDS | 25.0% |
| ! | TTATATGTTGGATGAAGAAT+GGG | + | chr8.2:30147133-30147152 | MS.gene28903:CDS | 25.0% |
| ! | TTCAACAAGTTCCATATAAT+TGG | - | chr8.2:30146651-30146670 | None:intergenic | 25.0% |
| ! | TTTATATGTTGGATGAAGAA+TGG | + | chr8.2:30147132-30147151 | MS.gene28903:CDS | 25.0% |
| !!! | ATGGAACTTGTTGAATTATA+GGG | + | chr8.2:30146656-30146675 | MS.gene28903:CDS | 25.0% |
| !!! | TATGGAACTTGTTGAATTAT+AGG | + | chr8.2:30146655-30146674 | MS.gene28903:CDS | 25.0% |
| AAAGGAAAAGAAGGAGAATT+TGG | - | chr8.2:30146393-30146412 | None:intergenic | 30.0% | |
| AATGCAAGGATCAAAATCAA+CGG | - | chr8.2:30147327-30147346 | None:intergenic | 30.0% | |
| ACAACAGTTCTCCAATTATA+TGG | + | chr8.2:30146637-30146656 | MS.gene28903:CDS | 30.0% | |
| ATAAATGGGGAACTATTTCA+TGG | + | chr8.2:30146922-30146941 | MS.gene28903:CDS | 30.0% | |
| ATCTTTCAGGAAGGTAAATT+AGG | + | chr8.2:30146801-30146820 | MS.gene28903:CDS | 30.0% | |
| ATTGATGGAGGTAATTGTAT+AGG | - | chr8.2:30146443-30146462 | None:intergenic | 30.0% | |
| GATAAATAGTTGATTGATGG+AGG | - | chr8.2:30146455-30146474 | None:intergenic | 30.0% | |
| GATGATGTCATTGTATTCAA+GGG | + | chr8.2:30146882-30146901 | MS.gene28903:CDS | 30.0% | |
| GGTATTTGTAAAGGAAAAGA+AGG | - | chr8.2:30146402-30146421 | None:intergenic | 30.0% | |
| GTTTGGAAACAAGAAACATT+TGG | + | chr8.2:30146995-30147014 | MS.gene28903:CDS | 30.0% | |
| TAAAGGTGGTAAATTGAAGT+TGG | - | chr8.2:30146502-30146521 | None:intergenic | 30.0% | |
| TATATGTTGGATGAAGAATG+GGG | + | chr8.2:30147134-30147153 | MS.gene28903:CDS | 30.0% | |
| TGAAATATGGAGACACAAAA+TGG | + | chr8.2:30146829-30146848 | MS.gene28903:CDS | 30.0% | |
| TTGATGGAGGTAATTGTATA+GGG | - | chr8.2:30146442-30146461 | None:intergenic | 30.0% | |
| ! | AGGATATGATAATTTGCAGT+CGG | - | chr8.2:30146607-30146626 | None:intergenic | 30.0% |
| ! | GTTGTTGATAGATACTATGA+AGG | + | chr8.2:30147041-30147060 | MS.gene28903:CDS | 30.0% |
| ! | TGATGATGTCATTGTATTCA+AGG | + | chr8.2:30146881-30146900 | MS.gene28903:CDS | 30.0% |
| AACCAACCTTTGGTATAAGA+AGG | - | chr8.2:30146528-30146547 | None:intergenic | 35.0% | |
| AACTCTCCTTGGATTAATGT+TGG | + | chr8.2:30146759-30146778 | MS.gene28903:CDS | 35.0% | |
| AAGTTGGAACTTGCATTAGT+TGG | - | chr8.2:30146486-30146505 | None:intergenic | 35.0% | |
| ACATTAATCCAAGGAGAGTT+AGG | - | chr8.2:30146759-30146778 | None:intergenic | 35.0% | |
| ACTCTCCTTGGATTAATGTT+GGG | + | chr8.2:30146760-30146779 | MS.gene28903:CDS | 35.0% | |
| AGACACAAAATGGAAACTTG+TGG | + | chr8.2:30146839-30146858 | MS.gene28903:CDS | 35.0% | |
| AGGGAATTGCATCTACTTTA+AGG | + | chr8.2:30147253-30147272 | MS.gene28903:CDS | 35.0% | |
| ATATGTTGGATGAAGAATGG+GGG | + | chr8.2:30147135-30147154 | MS.gene28903:CDS | 35.0% | |
| ATCAAGATACTTCCCTATCT+TGG | - | chr8.2:30146301-30146320 | None:intergenic | 35.0% | |
| CTCACAAAACTTGATGAGTA+TGG | - | chr8.2:30146720-30146739 | None:intergenic | 35.0% | |
| GGATTCAACAAACTCTTCAA+AGG | - | chr8.2:30146579-30146598 | None:intergenic | 35.0% | |
| TAATGTTCGTATGTACAGCT+TGG | + | chr8.2:30147283-30147302 | MS.gene28903:CDS | 35.0% | |
| TGGAAACAAGAAACATTTGG+TGG | + | chr8.2:30146998-30147017 | MS.gene28903:CDS | 35.0% | |
| TTAGCACCTTCTTATACCAA+AGG | + | chr8.2:30146519-30146538 | MS.gene28903:CDS | 35.0% | |
| ! | AAACATTTGGTGGAATCTTG+TGG | + | chr8.2:30147008-30147027 | MS.gene28903:CDS | 35.0% |
| ! | AACATTTGGTGGAATCTTGT+GGG | + | chr8.2:30147009-30147028 | MS.gene28903:CDS | 35.0% |
| ! | AATTTTCACCTCCTTTGTGT+GGG | + | chr8.2:30146973-30146992 | MS.gene28903:CDS | 35.0% |
| ! | ACAACTGCTGCTTAAAATGA+AGG | - | chr8.2:30147195-30147214 | None:intergenic | 35.0% |
| !! | TGTAGCTTGTTGTATCTTTC+AGG | + | chr8.2:30146788-30146807 | MS.gene28903:CDS | 35.0% |
| !! | TTGGTATAAGAAGGTGCTAA+AGG | - | chr8.2:30146519-30146538 | None:intergenic | 35.0% |
| AAAGAGCTATGGTCCAAGAT+AGG | + | chr8.2:30146285-30146304 | MS.gene28903:CDS | 40.0% | |
| AAGAGCTATGGTCCAAGATA+GGG | + | chr8.2:30146286-30146305 | MS.gene28903:CDS | 40.0% | |
| AGAAGGAGAATTTGGAAGAG+AGG | - | chr8.2:30146385-30146404 | None:intergenic | 40.0% | |
| AGGTAATTGTATAGGGAGAC+AGG | - | chr8.2:30146435-30146454 | None:intergenic | 40.0% | |
| ATCTTGGACCATAGCTCTTT+GGG | - | chr8.2:30146285-30146304 | None:intergenic | 40.0% | |
| ATGAAGGTGAAACCATGAGA+GGG | + | chr8.2:30147057-30147076 | MS.gene28903:CDS | 40.0% | |
| CAATCCCCAACATTAATCCA+AGG | - | chr8.2:30146768-30146787 | None:intergenic | 40.0% | |
| CACCTTCTTATACCAAAGGT+TGG | + | chr8.2:30146523-30146542 | MS.gene28903:CDS | 40.0% | |
| CACTTTGATCAACCAACCTT+TGG | - | chr8.2:30146538-30146557 | None:intergenic | 40.0% | |
| CTCTCCTTGGATTAATGTTG+GGG | + | chr8.2:30146761-30146780 | MS.gene28903:CDS | 40.0% | |
| CTTTGTGGTTTCATTCACGT+TGG | + | chr8.2:30147354-30147373 | MS.gene28903:CDS | 40.0% | |
| GAACTGTTGTTACTGTCAAG+AGG | - | chr8.2:30146627-30146646 | None:intergenic | 40.0% | |
| GAAGGAGAATTTGGAAGAGA+GGG | - | chr8.2:30146384-30146403 | None:intergenic | 40.0% | |
| GGTAATTGTATAGGGAGACA+GGG | - | chr8.2:30146434-30146453 | None:intergenic | 40.0% | |
| TATCTTGGACCATAGCTCTT+TGG | - | chr8.2:30146286-30146305 | None:intergenic | 40.0% | |
| TATGAAGGTGAAACCATGAG+AGG | + | chr8.2:30147056-30147075 | MS.gene28903:CDS | 40.0% | |
| TGTCATATCCTAACTCTCCT+TGG | + | chr8.2:30146748-30146767 | MS.gene28903:CDS | 40.0% | |
| TTGTTTCCAAACCCACACAA+AGG | - | chr8.2:30146987-30147006 | None:intergenic | 40.0% | |
| ! | ATGGTGCGAGGTATTTGTAA+AGG | - | chr8.2:30146411-30146430 | None:intergenic | 40.0% |
| ! | CAAAGGTTGGTTGATCAAAG+TGG | + | chr8.2:30146536-30146555 | MS.gene28903:CDS | 40.0% |
| ! | CAATTTTCACCTCCTTTGTG+TGG | + | chr8.2:30146972-30146991 | MS.gene28903:CDS | 40.0% |
| ! | CACAAAGTCTTGTCAATGCA+AGG | - | chr8.2:30147341-30147360 | None:intergenic | 40.0% |
| ! | CTTGCATTGACAAGACTTTG+TGG | + | chr8.2:30147339-30147358 | MS.gene28903:CDS | 40.0% |
| ! | GATAATTTGCAGTCGGAGAT+AGG | - | chr8.2:30146600-30146619 | None:intergenic | 40.0% |
| !! | GCTTGTTGTATCTTTCAGGA+AGG | + | chr8.2:30146792-30146811 | MS.gene28903:CDS | 40.0% |
| !! | GTATAAGAAGGTGCTAAAGG+TGG | - | chr8.2:30146516-30146535 | None:intergenic | 40.0% |
| ACCATGAGAGGGAACAATGT+TGG | + | chr8.2:30147068-30147087 | MS.gene28903:CDS | 45.0% | |
| ATTGTATAGGGAGACAGGGA+TGG | - | chr8.2:30146430-30146449 | None:intergenic | 45.0% | |
| ATTTGGAAGAGAGGGAGGAA+TGG | - | chr8.2:30146376-30146395 | None:intergenic | 45.0% | |
| GGATGAAGAATGGGGGAAAT+GGG | + | chr8.2:30147142-30147161 | MS.gene28903:CDS | 45.0% | |
| TCCAACATTGTTCCCTCTCA+TGG | - | chr8.2:30147072-30147091 | None:intergenic | 45.0% | |
| TCTTGGACCATAGCTCTTTG+GGG | - | chr8.2:30146284-30146303 | None:intergenic | 45.0% | |
| TGGATGAAGAATGGGGGAAA+TGG | + | chr8.2:30147141-30147160 | MS.gene28903:CDS | 45.0% | |
| TTTCCAAACCCACACAAAGG+AGG | - | chr8.2:30146984-30147003 | None:intergenic | 45.0% | |
| CTCATCTCCCCAAAGAGCTA+TGG | + | chr8.2:30146274-30146293 | MS.gene28903:CDS | 50.0% | |
| GGAGAATTTGGAAGAGAGGG+AGG | - | chr8.2:30146381-30146400 | None:intergenic | 50.0% | |
| TCACCTCCTTTGTGTGGGTT+TGG | + | chr8.2:30146978-30146997 | MS.gene28903:CDS | 50.0% | |
| TGTTGGAAGACGCCATGATC+AGG | + | chr8.2:30147085-30147104 | MS.gene28903:CDS | 50.0% | |
| AAGACGCCATGATCAGGACG+CGG | + | chr8.2:30147091-30147110 | MS.gene28903:CDS | 55.0% | |
| CCACAACCGCGTCCTGATCA+TGG | - | chr8.2:30147100-30147119 | None:intergenic | 60.0% | |
| CCATGATCAGGACGCGGTTG+TGG | + | chr8.2:30147097-30147116 | MS.gene28903:CDS | 60.0% | |
| AGGGAGACAGGGATGGTGCG+AGG | - | chr8.2:30146423-30146442 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 30146252 | 30147388 | 30146252 | ID=MS.gene28903 |
| chr8.2 | mRNA | 30146252 | 30147388 | 30146252 | ID=MS.gene28903.t1;Parent=MS.gene28903 |
| chr8.2 | exon | 30146252 | 30147388 | 30146252 | ID=MS.gene28903.t1.exon1;Parent=MS.gene28903.t1 |
| chr8.2 | CDS | 30146252 | 30147388 | 30146252 | ID=cds.MS.gene28903.t1;Parent=MS.gene28903.t1 |
| Gene Sequence |
| Protein sequence |
