AlfalfaGEDB | MS.gene44190

M. sativa cultivar XinJiangDaYe / MS.gene44190

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF03478.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44190.t1	XP_003629211.1	94.7	397	21	0	1	397	1	397	3.70E-228	800.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44190.t1	Q3EBZ2	29.9	405	237	13	23	395	1	390	3.0e-37	157.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44190.t1	G7LA96	94.7	397	21	0	1	397	1	397	2.7e-228	800.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050645	MS.gene44190	0.822937	1.81E-53	-1.69E-46
MS.gene052637	MS.gene44190	0.827433	1.57E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene012159	MS.gene44190	PPI
MS.gene037197	MS.gene44190	PPI
MS.gene23495	MS.gene44190	PPI
MS.gene23497	MS.gene44190	PPI
MS.gene30094	MS.gene44190	PPI
MS.gene44190	MS.gene28903	PPI
MS.gene44190	MS.gene025209	PPI
MS.gene44190	MS.gene28904	PPI
MS.gene44190	MS.gene44191	PPI
MS.gene44190	MS.gene30094	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44190.t1	MTR_8g074610	94.710	397	21	0	1	397	1	397	0.0	761
MS.gene44190.t1	MTR_2g041510	76.500	400	83	5	1	397	1	392	0.0	592
MS.gene44190.t1	MTR_2g041510	76.053	380	81	4	20	397	1	372	0.0	581
MS.gene44190.t1	MTR_8g074640	74.142	379	95	3	20	397	1	377	0.0	579
MS.gene44190.t1	MTR_6g092570	33.333	375	203	10	21	383	4	343	1.90e-49	171
MS.gene44190.t1	MTR_8g085760	32.648	389	214	12	21	383	9	375	3.22e-49	171
MS.gene44190.t1	MTR_5g079830	33.427	356	184	9	20	359	1	319	1.38e-47	167
MS.gene44190.t1	MTR_7g096720	35.215	372	180	16	20	359	6	348	3.06e-46	163
MS.gene44190.t1	MTR_4g088925	33.780	373	191	12	33	383	34	372	3.75e-45	160
MS.gene44190.t1	MTR_3g030050	31.163	430	227	15	16	395	10	420	1.43e-43	157

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44190.t1	AT2G17030	29.877	405	237	13	23	395	1	390	3.16e-41	150
MS.gene44190.t1	AT2G17036	28.571	406	235	16	23	395	1	384	4.85e-36	136
MS.gene44190.t1	AT2G17036	28.642	405	234	16	24	395	1	383	7.76e-36	135
MS.gene44190.t1	AT1G65770	28.610	367	212	13	23	372	1	334	2.56e-34	130
MS.gene44190.t1	AT4G35733	29.124	388	214	15	20	384	1	350	1.88e-33	128
MS.gene44190.t1	AT2G26160	30.312	353	193	14	25	361	5	320	1.64e-29	117
MS.gene44190.t1	AT1G44080	28.533	368	210	13	24	381	4	328	2.25e-29	117
MS.gene44190.t1	AT3G25750	27.688	372	220	11	22	376	2	341	1.14e-27	112
MS.gene44190.t1	AT3G25750	28.378	370	206	13	22	376	2	327	2.03e-26	108
MS.gene44190.t1	AT2G17690	28.910	422	215	15	25	379	3	406	1.08e-25	108

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Find 72 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACTTAATTGGATATCA+AGG	0.406036	8.1:+32152268	MS.gene44190:CDS
AAAGTAGTGAAGACATGGGA+AGG	0.643211	8.1:+32152092	MS.gene44190:CDS
AAATTAGGATTCATGAAACA+TGG	0.629944	8.1:+32151848	MS.gene44190:CDS
AAGACTTAATTGGATATCAA+GGG	0.598333	8.1:+32152269	MS.gene44190:CDS
AAGCATTTGATGGAGTCATG+TGG	0.661502	8.1:+32152040	MS.gene44190:CDS
AAGGGAACTGCATCTACTTT+AGG	0.247416	8.1:+32152287	MS.gene44190:CDS
AAGTGTTGTTACTGCTAAGA+GGG	0.570047	8.1:-32151655	None:intergenic
AATGGAAAAGAAGGAGAGTT+TGG	0.332443	8.1:-32151425	None:intergenic
ACAACACTTCTACCATGCTA+TGG	0.549319	8.1:+32151669	MS.gene44190:CDS
ACACTAGTCCAAGGAGAGTT+AGG	0.390020	8.1:-32151788	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAAATGGGGAACTATTTCA+TGG	+	chr8.1:32151954-32151973	MS.gene44190:CDS	30.0%
ATCTTTCAAGAGGGTAAATT+AGG	+	chr8.1:32151833-32151852	MS.gene44190:CDS	30.0%
CAATGATATAAACGGTTGAT+TGG	-	chr8.1:32151496-32151515	None:intergenic	30.0%
GTCTCAACTAAAGACTTAAT+TGG	+	chr8.1:32152259-32152278	MS.gene44190:CDS	30.0%
AATGGAAAAGAAGGAGAGTT+TGG	-	chr8.1:32151428-32151447	None:intergenic	35.0%
ATAGAACTATGGCCTAAGAT+AGG	+	chr8.1:32151320-32151339	MS.gene44190:CDS	35.0%
ATAGGACTCAACAAACTTAC+AGG	-	chr8.1:32151614-32151633	None:intergenic	35.0%
ATCTGATTCTTCTTCATCCA+TGG	+	chr8.1:32151268-32151287	MS.gene44190:CDS	35.0%
CTCAACTTCCTATAGAACTA+TGG	+	chr8.1:32151309-32151328	MS.gene44190:CDS	35.0%
GACGATGTTATTGTGTTCAA+GGG	+	chr8.1:32151914-32151933	MS.gene44190:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	32151230	32152423	32151230	ID=MS.gene44190
chr8.1	mRNA	32151230	32152423	32151230	ID=MS.gene44190.t1;Parent=MS.gene44190
chr8.1	exon	32151230	32152423	32151230	ID=MS.gene44190.t1.exon1;Parent=MS.gene44190.t1
chr8.1	CDS	32151230	32152423	32151230	ID=cds.MS.gene44190.t1;Parent=MS.gene44190.t1

Gene Sequence

>MS.gene44190

ATGATATCACTCTTCAGCAAAATCAACAAATCTTTGAGATCTGATTCTTCTTCATCCATGGATGAGATAGTTGATTGGTCTCAACTTCCTATAGAACTATGGCCTAAGATAGGAAAATATCTTGATAAACATATAGATGTTCTCAGATTTCGCAGTGTCTGCGAATCATTTCGTTCTTCCATTCCTCCATCTCATCCAAACTCTCCTTCTTTTCCATTACAAATACCTCACCCTGTCTACGACTCGCTAAACAACTACATCAACCAATCAACCGTTTATATCATTGAACCAACTGATGCAAGTTCCAACTCCAATTTAGAACCTTTAGCACCTTCTTCTTCCAAAGGTTGGCTGATTAAGGTAGAAGAAACAAAGAATCAACCTGTAAGTTTGTTGAGTCCTATCTCTGACCGAAAATTATCATACCCTCTTAGCAGTAACAACACTTCTACCATGCTATGGAACTTGTTGGATCATAGGGTCGTTGAGCTATGCAAATCATATAAAATTGAAAAAACTACACGTTTCTCTAGTTCTGCTATCAAAGTTGTGTTCTATCCTAACTCTCCTTGGACTAGTGTTGAGGAATGTGTAGCTTGTTGTATCTTTCAAGAGGGTAAATTAGGATTCATGAAACATGGTGATGAAAAATGGACACTTGTGGATAACAAGAATTTCTACTATGACGATGTTATTGTGTTCAAGGGTCAGTTTTATGTTACTGATAAATGGGGAACTATTTCATGGATTGATGTCTCTACTTTGAGATTGATACAATTCTCACCTCCTTTGTGTGGATTTGGAAACAAGAAGCATTTGATGGAGTCATGTGGAAGTCTTTACGTCGTTGACAGATACTGTGAAAGTAGTGAAGACATGGGAAGGAACTATGTTAGAAGACGCTACGATCGAGACACTGATGTGGAATGTTTCAAAGTTTATAAGTTGGATGAAGAATGGGGAAAATGGGTTGATGTGAAAAGCTTGAGAGATAGAGCTTTCATTTTGAGCGACAGTTGTAACTTCTCTGTCTCAACTAAAGACTTAATTGGATATCAAGGGAACTGCATCTACTTTAGGGATACTTATGATGTTCGTATGTATAATTTGGATGATCATAGCATCACTATGGTTAATTTTAATCCTTGTATTGACAAGACTTTGTGGTCTCGTTCACCTTGGATGAGATGTTAG

Protein sequence

>MS.gene44190.t1

MISLFSKINKSLRSDSSSSMDEIVDWSQLPIELWPKIGKYLDKHIDVLRFRSVCESFRSSIPPSHPNSPSFPLQIPHPVYDSLNNYINQSTVYIIEPTDASSNSNLEPLAPSSSKGWLIKVEETKNQPVSLLSPISDRKLSYPLSSNNTSTMLWNLLDHRVVELCKSYKIEKTTRFSSSAIKVVFYPNSPWTSVEECVACCIFQEGKLGFMKHGDEKWTLVDNKNFYYDDVIVFKGQFYVTDKWGTISWIDVSTLRLIQFSPPLCGFGNKKHLMESCGSLYVVDRYCESSEDMGRNYVRRRYDRDTDVECFKVYKLDEEWGKWVDVKSLRDRAFILSDSCNFSVSTKDLIGYQGNCIYFRDTYDVRMYNLDDHSITMVNFNPCIDKTLWSRSPWMRC