Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44191.t1 | XP_003629214.1 | 91.5 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 8.20E-209 | 736.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44191.t1 | Q9SHX9 | 30.4 | 358 | 213 | 10 | 6 | 353 | 3 | 334 | 4.8e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44191.t1 | G7LA99 | 91.5 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 5.9e-209 | 736.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene44191 | MS.gene28904 | PPI |
| MS.gene44191 | MS.gene39445 | PPI |
| MS.gene44190 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene08770 | PPI |
| MS.gene23495 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene66307 | PPI |
| MS.gene44191 | MS.gene023531 | PPI |
| MS.gene012159 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene018718 | PPI |
| MS.gene97013 | MS.gene44191 | PPI |
| MS.gene64423 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene049886 | PPI |
| MS.gene44191 | MS.gene017581 | PPI |
| MS.gene037197 | MS.gene44191 | PPI |
| MS.gene91760 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene012160 | PPI |
| MS.gene44191 | MS.gene017582 | PPI |
| MS.gene28903 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene064059 | PPI |
| MS.gene23497 | MS.gene44191 | PPI |
| MS.gene44191 | MS.gene025209 | PPI |
| MS.gene30094 | MS.gene44191 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44191.t1 | MTR_8g074640 | 91.534 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 0.0 | 706 |
| MS.gene44191.t1 | MTR_8g074610 | 77.105 | 380 | 83 | 4 | 1 | 378 | 20 | 397 | 0.0 | 595 |
| MS.gene44191.t1 | MTR_2g041510 | 76.115 | 381 | 79 | 5 | 1 | 378 | 21 | 392 | 0.0 | 559 |
| MS.gene44191.t1 | MTR_2g041510 | 76.115 | 381 | 79 | 5 | 1 | 378 | 1 | 372 | 0.0 | 559 |
| MS.gene44191.t1 | MTR_5g079830 | 35.593 | 354 | 179 | 12 | 1 | 340 | 1 | 319 | 1.25e-51 | 177 |
| MS.gene44191.t1 | MTR_6g092570 | 34.211 | 380 | 193 | 11 | 2 | 364 | 4 | 343 | 8.24e-50 | 171 |
| MS.gene44191.t1 | MTR_8g085760 | 33.684 | 380 | 201 | 12 | 2 | 354 | 9 | 364 | 8.46e-49 | 169 |
| MS.gene44191.t1 | MTR_7g096720 | 32.682 | 358 | 192 | 11 | 8 | 340 | 15 | 348 | 2.89e-45 | 160 |
| MS.gene44191.t1 | MTR_4g088925 | 32.258 | 372 | 198 | 12 | 14 | 364 | 34 | 372 | 1.74e-39 | 144 |
| MS.gene44191.t1 | MTR_1g026850 | 30.227 | 397 | 222 | 13 | 1 | 376 | 1 | 363 | 2.55e-36 | 135 |
| MS.gene44191.t1 | MTR_3g030050 | 30.166 | 421 | 230 | 14 | 5 | 376 | 15 | 420 | 4.91e-35 | 133 |
| MS.gene44191.t1 | MTR_6g018390 | 29.722 | 360 | 189 | 13 | 5 | 340 | 4 | 323 | 5.94e-30 | 118 |
| MS.gene44191.t1 | MTR_3g058460 | 31.443 | 388 | 222 | 15 | 6 | 376 | 15 | 375 | 3.29e-29 | 116 |
| MS.gene44191.t1 | MTR_3g030360 | 29.620 | 368 | 204 | 14 | 3 | 351 | 2 | 333 | 4.57e-28 | 113 |
| MS.gene44191.t1 | MTR_3g029650 | 25.956 | 366 | 186 | 8 | 5 | 340 | 4 | 314 | 1.86e-27 | 114 |
| MS.gene44191.t1 | MTR_3g029650 | 25.556 | 360 | 228 | 13 | 4 | 342 | 370 | 710 | 8.94e-20 | 91.7 |
| MS.gene44191.t1 | MTR_1g007260 | 27.907 | 387 | 210 | 15 | 5 | 359 | 21 | 370 | 2.79e-26 | 108 |
| MS.gene44191.t1 | MTR_2g047180 | 28.205 | 390 | 235 | 14 | 5 | 376 | 3 | 365 | 4.50e-26 | 108 |
| MS.gene44191.t1 | MTR_4g125090 | 28.354 | 328 | 172 | 11 | 2 | 321 | 41 | 313 | 1.35e-25 | 105 |
| MS.gene44191.t1 | MTR_3g029620 | 31.680 | 363 | 193 | 16 | 5 | 345 | 2 | 331 | 1.96e-25 | 106 |
| MS.gene44191.t1 | MTR_6g027040 | 27.930 | 401 | 235 | 18 | 5 | 378 | 4 | 377 | 1.24e-24 | 103 |
| MS.gene44191.t1 | MTR_1g016570 | 29.070 | 344 | 187 | 12 | 6 | 342 | 21 | 314 | 7.50e-24 | 101 |
| MS.gene44191.t1 | MTR_3g029920 | 29.275 | 386 | 204 | 16 | 4 | 361 | 7 | 351 | 8.86e-24 | 101 |
| MS.gene44191.t1 | MTR_6g027100 | 28.678 | 401 | 232 | 19 | 5 | 378 | 4 | 377 | 9.29e-24 | 102 |
| MS.gene44191.t1 | MTR_3g030440 | 28.530 | 347 | 209 | 12 | 1 | 342 | 1 | 313 | 2.19e-23 | 100 |
| MS.gene44191.t1 | MTR_6g018400 | 26.768 | 396 | 201 | 15 | 3 | 362 | 2 | 344 | 5.13e-23 | 99.4 |
| MS.gene44191.t1 | MTR_3g030480 | 29.226 | 349 | 196 | 11 | 5 | 342 | 4 | 312 | 6.11e-23 | 99.0 |
| MS.gene44191.t1 | MTR_3g030380 | 26.801 | 347 | 211 | 9 | 4 | 342 | 3 | 314 | 3.14e-22 | 97.1 |
| MS.gene44191.t1 | MTR_3g029640 | 28.533 | 368 | 188 | 15 | 5 | 345 | 3 | 322 | 7.29e-22 | 96.3 |
| MS.gene44191.t1 | MTR_6g018360 | 29.086 | 361 | 204 | 17 | 5 | 342 | 4 | 335 | 1.12e-21 | 96.3 |
| MS.gene44191.t1 | MTR_1g049170 | 28.117 | 377 | 199 | 15 | 1 | 342 | 1 | 340 | 2.09e-21 | 95.1 |
| MS.gene44191.t1 | MTR_3g037710 | 36.905 | 168 | 98 | 3 | 180 | 342 | 55 | 219 | 9.68e-21 | 91.3 |
| MS.gene44191.t1 | MTR_3g114990 | 27.000 | 400 | 232 | 16 | 5 | 374 | 22 | 391 | 1.21e-20 | 93.2 |
| MS.gene44191.t1 | MTR_3g029840 | 28.134 | 359 | 194 | 16 | 5 | 342 | 4 | 319 | 1.38e-20 | 92.4 |
| MS.gene44191.t1 | MTR_3g030080 | 27.095 | 358 | 202 | 15 | 6 | 342 | 7 | 326 | 3.28e-20 | 91.3 |
| MS.gene44191.t1 | MTR_3g029950 | 27.824 | 363 | 197 | 16 | 5 | 345 | 4 | 323 | 1.43e-19 | 89.4 |
| MS.gene44191.t1 | MTR_3g114970 | 25.337 | 371 | 231 | 16 | 5 | 363 | 3 | 339 | 2.20e-19 | 89.0 |
| MS.gene44191.t1 | MTR_3g029670 | 24.390 | 369 | 221 | 9 | 5 | 343 | 4 | 344 | 5.91e-19 | 87.8 |
| MS.gene44191.t1 | MTR_3g030355 | 26.812 | 414 | 211 | 18 | 1 | 359 | 1 | 377 | 2.86e-18 | 85.9 |
| MS.gene44191.t1 | MTR_3g091990 | 26.648 | 364 | 212 | 13 | 4 | 340 | 5 | 340 | 2.99e-18 | 86.3 |
| MS.gene44191.t1 | MTR_3g029750 | 23.545 | 378 | 229 | 8 | 5 | 351 | 4 | 352 | 4.20e-18 | 85.5 |
| MS.gene44191.t1 | MTR_3g029820 | 28.934 | 394 | 160 | 17 | 4 | 370 | 7 | 307 | 2.30e-16 | 79.7 |
| MS.gene44191.t1 | MTR_3g047980 | 27.926 | 376 | 220 | 16 | 6 | 376 | 30 | 359 | 7.96e-16 | 78.6 |
| MS.gene44191.t1 | MTR_6g018315 | 26.667 | 390 | 231 | 19 | 18 | 377 | 2 | 366 | 2.66e-15 | 77.0 |
| MS.gene44191.t1 | MTR_3g029900 | 28.846 | 312 | 159 | 14 | 68 | 368 | 177 | 436 | 5.13e-15 | 76.6 |
| MS.gene44191.t1 | MTR_1g016540 | 34.965 | 143 | 75 | 4 | 207 | 342 | 2 | 133 | 4.38e-12 | 64.7 |
| MS.gene44191.t1 | MTR_6g018370 | 33.594 | 128 | 76 | 4 | 192 | 318 | 132 | 251 | 5.84e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44191.t1 | AT2G17030 | 31.135 | 379 | 224 | 13 | 5 | 361 | 2 | 365 | 2.85e-40 | 147 |
| MS.gene44191.t1 | AT1G65770 | 30.252 | 357 | 219 | 11 | 5 | 354 | 2 | 335 | 6.37e-36 | 134 |
| MS.gene44191.t1 | AT4G35733 | 29.600 | 375 | 208 | 12 | 1 | 356 | 1 | 338 | 6.66e-35 | 132 |
| MS.gene44191.t1 | AT2G17036 | 27.614 | 373 | 239 | 11 | 5 | 361 | 2 | 359 | 1.70e-29 | 118 |
| MS.gene44191.t1 | AT2G17036 | 27.614 | 373 | 239 | 11 | 5 | 361 | 1 | 358 | 1.80e-29 | 118 |
| MS.gene44191.t1 | AT3G25750 | 28.760 | 379 | 205 | 14 | 3 | 356 | 2 | 340 | 3.63e-29 | 116 |
| MS.gene44191.t1 | AT1G44080 | 27.688 | 372 | 202 | 10 | 4 | 358 | 3 | 324 | 5.92e-29 | 115 |
| MS.gene44191.t1 | AT1G65740 | 29.041 | 365 | 225 | 12 | 5 | 354 | 2 | 347 | 4.08e-27 | 110 |
| MS.gene44191.t1 | AT3G25750 | 28.117 | 377 | 196 | 13 | 3 | 356 | 2 | 326 | 5.55e-26 | 107 |
| MS.gene44191.t1 | AT2G26160 | 28.531 | 354 | 204 | 12 | 3 | 342 | 2 | 320 | 5.75e-25 | 104 |
| MS.gene44191.t1 | AT1G65760 | 28.686 | 373 | 216 | 15 | 6 | 359 | 5 | 346 | 1.40e-23 | 100 |
| MS.gene44191.t1 | AT2G17690 | 27.423 | 423 | 218 | 14 | 6 | 359 | 3 | 405 | 1.47e-21 | 95.9 |
| MS.gene44191.t1 | AT2G16290 | 24.103 | 390 | 236 | 14 | 5 | 356 | 2 | 369 | 1.50e-18 | 87.0 |
| MS.gene44191.t1 | AT5G60060 | 28.307 | 378 | 219 | 19 | 7 | 361 | 11 | 359 | 3.28e-18 | 85.5 |
| MS.gene44191.t1 | AT1G64840 | 26.891 | 357 | 208 | 16 | 6 | 342 | 4 | 327 | 1.73e-16 | 80.5 |
| MS.gene44191.t1 | AT5G24040 | 23.499 | 383 | 198 | 17 | 5 | 346 | 2 | 330 | 1.11e-13 | 72.0 |
| MS.gene44191.t1 | AT1G57906 | 29.545 | 176 | 84 | 6 | 187 | 329 | 58 | 226 | 3.40e-12 | 65.9 |
Find 87 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAGCTTGTTGTATCTTTC+AGG | 0.173014 | 8.1:+32140957 | MS.gene44191:CDS |
| TTCAACAAGTTCCATATAAT+TGG | 0.175089 | 8.1:-32140817 | None:intergenic |
| AGGGAATTGCATCTACTTTA+AGG | 0.185868 | 8.1:+32141422 | MS.gene44191:CDS |
| TATGGAACTTGTTGAATTAT+AGG | 0.195427 | 8.1:+32140824 | MS.gene44191:CDS |
| AAAGGAAAAGAAGGAGAATT+TGG | 0.229744 | 8.1:-32140559 | None:intergenic |
| GTTTCTGCTAAAGAATTAAT+TGG | 0.230909 | 8.1:+32141393 | MS.gene44191:CDS |
| ATCTTGGACCATAGCTCTTT+GGG | 0.243195 | 8.1:-32140451 | None:intergenic |
| ATCTTTCAGGAAGGTAAATT+AGG | 0.270135 | 8.1:+32140970 | MS.gene44191:CDS |
| TATCTTGGACCATAGCTCTT+TGG | 0.291416 | 8.1:-32140452 | None:intergenic |
| ATGGAACTTGTTGAATTATA+GGG | 0.301160 | 8.1:+32140825 | MS.gene44191:CDS |
| TCACCTCCTTTGTGTGGGTT+TGG | 0.311829 | 8.1:+32141147 | MS.gene44191:CDS |
| TGAAATATGGAGACACAAAA+TGG | 0.318037 | 8.1:+32140998 | MS.gene44191:CDS |
| AAATTAGGATTCATGAAATA+TGG | 0.332072 | 8.1:+32140985 | MS.gene44191:CDS |
| ATCAAGATACTTCCCTATCT+TGG | 0.333662 | 8.1:-32140467 | None:intergenic |
| ACAACAGTTCTCCAATTATA+TGG | 0.333718 | 8.1:+32140806 | MS.gene44191:CDS |
| TTGATAGAGGTAATTGTATA+GGG | 0.347302 | 8.1:-32140608 | None:intergenic |
| CTCACAAAACTTGATGAGTA+TGG | 0.352709 | 8.1:-32140886 | None:intergenic |
| ACTCTCCTTGGATTAATGTT+GGG | 0.357299 | 8.1:+32140929 | MS.gene44191:CDS |
| ATTGATAGAGGTAATTGTAT+AGG | 0.365166 | 8.1:-32140609 | None:intergenic |
| CACTTTGATCAACCAACCTT+TGG | 0.369704 | 8.1:-32140704 | None:intergenic |
| AAAGAGCTATGGTCCAAGAT+AGG | 0.372622 | 8.1:+32140454 | MS.gene44191:CDS |
| ATAAATGGGGAACTATTTCA+TGG | 0.373052 | 8.1:+32141091 | MS.gene44191:CDS |
| CTTGCATTGACAAGACTTTG+TGG | 0.374534 | 8.1:+32141508 | MS.gene44191:CDS |
| AGGTAATTGTATAGGGAGAC+AGG | 0.376170 | 8.1:-32140601 | None:intergenic |
| ACATTAATCCAAGGAGAGTT+AGG | 0.379494 | 8.1:-32140925 | None:intergenic |
| AAAGAATTAATTGGATATCA+AGG | 0.389911 | 8.1:+32141402 | MS.gene44191:CDS |
| GATAATTTGCAGTCGGAGAT+AGG | 0.391629 | 8.1:-32140766 | None:intergenic |
| TTTATATGTTGGATGAAGAA+TGG | 0.404503 | 8.1:+32141301 | MS.gene44191:CDS |
| CCATGATCGGGACGCGGTTG+TGG | 0.420452 | 8.1:+32141266 | MS.gene44191:CDS |
| ATTTGGAAGAGAGGGAGGAA+TGG | 0.422238 | 8.1:-32140542 | None:intergenic |
| TGTCCTATCCTAACTCTCCT+TGG | 0.428803 | 8.1:+32140917 | MS.gene44191:CDS |
| TGATGATGTCATTGTATTCA+AGG | 0.430249 | 8.1:+32141050 | MS.gene44191:CDS |
| TTATATGTTGGATGAAGAAT+GGG | 0.432756 | 8.1:+32141302 | MS.gene44191:CDS |
| GATAAATAGTTGATTGATAG+AGG | 0.439760 | 8.1:-32140621 | None:intergenic |
| CTCTCCTTGGATTAATGTTG+GGG | 0.439895 | 8.1:+32140930 | MS.gene44191:CDS |
| AACATTTGGTGGAATCTTGT+GGG | 0.442266 | 8.1:+32141178 | MS.gene44191:CDS |
| GTTTGGAAACAAGAAACATT+TGG | 0.451619 | 8.1:+32141164 | MS.gene44191:CDS |
| AACTCTCCTTGGATTAATGT+TGG | 0.455668 | 8.1:+32140928 | MS.gene44191:CDS |
| GAAGGAGAATTTGGAAGAGA+GGG | 0.458355 | 8.1:-32140550 | None:intergenic |
| AAGTTGGAACTTGCATTAGT+TGG | 0.460926 | 8.1:-32140652 | None:intergenic |
| AAACATTTGGTGGAATCTTG+TGG | 0.483180 | 8.1:+32141177 | MS.gene44191:CDS |
| CACGTTGGATGAGATGTTAG+AGG | 0.485509 | 8.1:+32141538 | MS.gene44191:CDS |
| TGTTGGAAGACGCCATGATC+GGG | 0.485748 | 8.1:+32141254 | MS.gene44191:CDS |
| AGAAGGAGAATTTGGAAGAG+AGG | 0.489920 | 8.1:-32140551 | None:intergenic |
| CCACAACCGCGTCCCGATCA+TGG | 0.492597 | 8.1:-32141266 | None:intergenic |
| GGTATTTGTAAAGGAAAAGA+AGG | 0.498247 | 8.1:-32140568 | None:intergenic |
| ACAACTGCTGCTTAAAATGA+AGG | 0.500009 | 8.1:-32141361 | None:intergenic |
| CACCTTCTTATACCAAAGGT+TGG | 0.500177 | 8.1:+32140692 | MS.gene44191:CDS |
| TAATGCTCGTATGTACAGCT+TGG | 0.504456 | 8.1:+32141452 | MS.gene44191:CDS |
| GGAGAATTTGGAAGAGAGGG+AGG | 0.512512 | 8.1:-32140547 | None:intergenic |
| AACCAACCTTTGGTATAAGA+AGG | 0.522904 | 8.1:-32140694 | None:intergenic |
| AACATGAGAGGGAACAATGT+TGG | 0.526890 | 8.1:+32141237 | MS.gene44191:CDS |
| TAAAGGTGGTAAATTGAAGT+TGG | 0.535440 | 8.1:-32140668 | None:intergenic |
| TTGGTATAAGAAGGTGCTAA+AGG | 0.538170 | 8.1:-32140685 | None:intergenic |
| AAGAGCTATGGTCCAAGATA+GGG | 0.546977 | 8.1:+32140455 | MS.gene44191:CDS |
| CTCATCTCCCCAAAGAGCTA+TGG | 0.548235 | 8.1:+32140443 | MS.gene44191:CDS |
| GGACTCAACAAACTCTTCAA+AGG | 0.551008 | 8.1:-32140745 | None:intergenic |
| GTGTTTCAAAGTTTATATGT+TGG | 0.555002 | 8.1:+32141290 | MS.gene44191:CDS |
| ATGTTGGAAGACGCCATGAT+CGG | 0.555148 | 8.1:+32141253 | MS.gene44191:CDS |
| GGTAATTGTATAGGGAGACA+GGG | 0.555775 | 8.1:-32140600 | None:intergenic |
| AAGAATTAATTGGATATCAA+GGG | 0.561141 | 8.1:+32141403 | MS.gene44191:CDS |
| ATGGTGCGAGGTATTTGTAA+AGG | 0.569151 | 8.1:-32140577 | None:intergenic |
| TGGAAACAAGAAACATTTGG+TGG | 0.570071 | 8.1:+32141167 | MS.gene44191:CDS |
| TTAGCACCTTCTTATACCAA+AGG | 0.570602 | 8.1:+32140688 | MS.gene44191:CDS |
| CAAAGGTTGGTTGATCAAAG+TGG | 0.574189 | 8.1:+32140705 | MS.gene44191:CDS |
| CACAAAGTCTTGTCAATGCA+AGG | 0.575490 | 8.1:-32141507 | None:intergenic |
| GTTGTTGATAGATACTATGA+AGG | 0.579405 | 8.1:+32141210 | MS.gene44191:CDS |
| ATGAAGGTGAAAACATGAGA+GGG | 0.582267 | 8.1:+32141226 | MS.gene44191:CDS |
| GATGATGTCATTGTATTCAA+GGG | 0.582955 | 8.1:+32141051 | MS.gene44191:CDS |
| AGGATATGATAATTTGCAGT+CGG | 0.584721 | 8.1:-32140773 | None:intergenic |
| CTTTGTGGTCTCATTCACGT+TGG | 0.590681 | 8.1:+32141523 | MS.gene44191:CDS |
| GCTTGTTGTATCTTTCAGGA+AGG | 0.596136 | 8.1:+32140961 | MS.gene44191:CDS |
| GTATAAGAAGGTGCTAAAGG+TGG | 0.604135 | 8.1:-32140682 | None:intergenic |
| AAGACGCCATGATCGGGACG+CGG | 0.613949 | 8.1:+32141260 | MS.gene44191:CDS |
| CAATCCCCAACATTAATCCA+AGG | 0.626001 | 8.1:-32140934 | None:intergenic |
| TCTTGGACCATAGCTCTTTG+GGG | 0.628895 | 8.1:-32140450 | None:intergenic |
| GAACTGTTGTTACTGTCAAG+AGG | 0.629902 | 8.1:-32140793 | None:intergenic |
| TATATGTTGGATGAAGAATG+GGG | 0.631666 | 8.1:+32141303 | MS.gene44191:CDS |
| ATTGTATAGGGAGACAGGGA+TGG | 0.639587 | 8.1:-32140596 | None:intergenic |
| AATCCAAGGAGAGTTAGGAT+AGG | 0.641071 | 8.1:-32140920 | None:intergenic |
| AGGGAGACAGGGATGGTGCG+AGG | 0.646104 | 8.1:-32140589 | None:intergenic |
| ATATGTTGGATGAAGAATGG+GGG | 0.646805 | 8.1:+32141304 | MS.gene44191:CDS |
| TATGAAGGTGAAAACATGAG+AGG | 0.647138 | 8.1:+32141225 | MS.gene44191:CDS |
| TTTCCAAACCCACACAAAGG+AGG | 0.652810 | 8.1:-32141150 | None:intergenic |
| AATGCAAGGATCAAAATCAA+CGG | 0.653913 | 8.1:-32141493 | None:intergenic |
| AGACACAAAATGGAAACTTG+TGG | 0.657681 | 8.1:+32141008 | MS.gene44191:CDS |
| TTGTTTCCAAACCCACACAA+AGG | 0.659541 | 8.1:-32141153 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATTAATTGGATATCA+AGG | + | chr8.1:32141402-32141421 | MS.gene44191:CDS | 20.0% |
| !! | AAATTAGGATTCATGAAATA+TGG | + | chr8.1:32140985-32141004 | MS.gene44191:CDS | 20.0% |
| !! | AAGAATTAATTGGATATCAA+GGG | + | chr8.1:32141403-32141422 | MS.gene44191:CDS | 20.0% |
| !!! | AGTTTTATGTTACTGATAAA+TGG | + | chr8.1:32141076-32141095 | MS.gene44191:CDS | 20.0% |
| !!! | GTTTTATGTTACTGATAAAT+GGG | + | chr8.1:32141077-32141096 | MS.gene44191:CDS | 20.0% |
| !!! | TTTTATGTTACTGATAAATG+GGG | + | chr8.1:32141078-32141097 | MS.gene44191:CDS | 20.0% |
| ! | ATTGATAGAGGTAATTGTAT+AGG | - | chr8.1:32140612-32140631 | None:intergenic | 25.0% |
| ! | GATAAATAGTTGATTGATAG+AGG | - | chr8.1:32140624-32140643 | None:intergenic | 25.0% |
| ! | GTGTTTCAAAGTTTATATGT+TGG | + | chr8.1:32141290-32141309 | MS.gene44191:CDS | 25.0% |
| ! | GTTTCTGCTAAAGAATTAAT+TGG | + | chr8.1:32141393-32141412 | MS.gene44191:CDS | 25.0% |
| ! | TTATATGTTGGATGAAGAAT+GGG | + | chr8.1:32141302-32141321 | MS.gene44191:CDS | 25.0% |
| ! | TTCAACAAGTTCCATATAAT+TGG | - | chr8.1:32140820-32140839 | None:intergenic | 25.0% |
| ! | TTGATAGAGGTAATTGTATA+GGG | - | chr8.1:32140611-32140630 | None:intergenic | 25.0% |
| ! | TTTATATGTTGGATGAAGAA+TGG | + | chr8.1:32141301-32141320 | MS.gene44191:CDS | 25.0% |
| !!! | ATGGAACTTGTTGAATTATA+GGG | + | chr8.1:32140825-32140844 | MS.gene44191:CDS | 25.0% |
| !!! | TATGGAACTTGTTGAATTAT+AGG | + | chr8.1:32140824-32140843 | MS.gene44191:CDS | 25.0% |
| AAAGGAAAAGAAGGAGAATT+TGG | - | chr8.1:32140562-32140581 | None:intergenic | 30.0% | |
| AATGCAAGGATCAAAATCAA+CGG | - | chr8.1:32141496-32141515 | None:intergenic | 30.0% | |
| ACAACAGTTCTCCAATTATA+TGG | + | chr8.1:32140806-32140825 | MS.gene44191:CDS | 30.0% | |
| ATAAATGGGGAACTATTTCA+TGG | + | chr8.1:32141091-32141110 | MS.gene44191:CDS | 30.0% | |
| ATCTTTCAGGAAGGTAAATT+AGG | + | chr8.1:32140970-32140989 | MS.gene44191:CDS | 30.0% | |
| GATGATGTCATTGTATTCAA+GGG | + | chr8.1:32141051-32141070 | MS.gene44191:CDS | 30.0% | |
| GGTATTTGTAAAGGAAAAGA+AGG | - | chr8.1:32140571-32140590 | None:intergenic | 30.0% | |
| GTTTGGAAACAAGAAACATT+TGG | + | chr8.1:32141164-32141183 | MS.gene44191:CDS | 30.0% | |
| TAAAGGTGGTAAATTGAAGT+TGG | - | chr8.1:32140671-32140690 | None:intergenic | 30.0% | |
| TATATGTTGGATGAAGAATG+GGG | + | chr8.1:32141303-32141322 | MS.gene44191:CDS | 30.0% | |
| TGAAATATGGAGACACAAAA+TGG | + | chr8.1:32140998-32141017 | MS.gene44191:CDS | 30.0% | |
| ! | AGGATATGATAATTTGCAGT+CGG | - | chr8.1:32140776-32140795 | None:intergenic | 30.0% |
| ! | GTTGTTGATAGATACTATGA+AGG | + | chr8.1:32141210-32141229 | MS.gene44191:CDS | 30.0% |
| ! | TGATGATGTCATTGTATTCA+AGG | + | chr8.1:32141050-32141069 | MS.gene44191:CDS | 30.0% |
| AACCAACCTTTGGTATAAGA+AGG | - | chr8.1:32140697-32140716 | None:intergenic | 35.0% | |
| AACTCTCCTTGGATTAATGT+TGG | + | chr8.1:32140928-32140947 | MS.gene44191:CDS | 35.0% | |
| AAGTTGGAACTTGCATTAGT+TGG | - | chr8.1:32140655-32140674 | None:intergenic | 35.0% | |
| ACATTAATCCAAGGAGAGTT+AGG | - | chr8.1:32140928-32140947 | None:intergenic | 35.0% | |
| ACTCTCCTTGGATTAATGTT+GGG | + | chr8.1:32140929-32140948 | MS.gene44191:CDS | 35.0% | |
| AGACACAAAATGGAAACTTG+TGG | + | chr8.1:32141008-32141027 | MS.gene44191:CDS | 35.0% | |
| AGGGAATTGCATCTACTTTA+AGG | + | chr8.1:32141422-32141441 | MS.gene44191:CDS | 35.0% | |
| ATATGTTGGATGAAGAATGG+GGG | + | chr8.1:32141304-32141323 | MS.gene44191:CDS | 35.0% | |
| ATCAAGATACTTCCCTATCT+TGG | - | chr8.1:32140470-32140489 | None:intergenic | 35.0% | |
| ATGAAGGTGAAAACATGAGA+GGG | + | chr8.1:32141226-32141245 | MS.gene44191:CDS | 35.0% | |
| CTCACAAAACTTGATGAGTA+TGG | - | chr8.1:32140889-32140908 | None:intergenic | 35.0% | |
| TATGAAGGTGAAAACATGAG+AGG | + | chr8.1:32141225-32141244 | MS.gene44191:CDS | 35.0% | |
| TGGAAACAAGAAACATTTGG+TGG | + | chr8.1:32141167-32141186 | MS.gene44191:CDS | 35.0% | |
| TTAGCACCTTCTTATACCAA+AGG | + | chr8.1:32140688-32140707 | MS.gene44191:CDS | 35.0% | |
| ! | AAACATTTGGTGGAATCTTG+TGG | + | chr8.1:32141177-32141196 | MS.gene44191:CDS | 35.0% |
| ! | AACATTTGGTGGAATCTTGT+GGG | + | chr8.1:32141178-32141197 | MS.gene44191:CDS | 35.0% |
| ! | AATTTTCACCTCCTTTGTGT+GGG | + | chr8.1:32141142-32141161 | MS.gene44191:CDS | 35.0% |
| ! | ACAACTGCTGCTTAAAATGA+AGG | - | chr8.1:32141364-32141383 | None:intergenic | 35.0% |
| !! | TGTAGCTTGTTGTATCTTTC+AGG | + | chr8.1:32140957-32140976 | MS.gene44191:CDS | 35.0% |
| !! | TTGGTATAAGAAGGTGCTAA+AGG | - | chr8.1:32140688-32140707 | None:intergenic | 35.0% |
| AAAGAGCTATGGTCCAAGAT+AGG | + | chr8.1:32140454-32140473 | MS.gene44191:CDS | 40.0% | |
| AACATGAGAGGGAACAATGT+TGG | + | chr8.1:32141237-32141256 | MS.gene44191:CDS | 40.0% | |
| AAGAGCTATGGTCCAAGATA+GGG | + | chr8.1:32140455-32140474 | MS.gene44191:CDS | 40.0% | |
| AATCCAAGGAGAGTTAGGAT+AGG | - | chr8.1:32140923-32140942 | None:intergenic | 40.0% | |
| AGAAGGAGAATTTGGAAGAG+AGG | - | chr8.1:32140554-32140573 | None:intergenic | 40.0% | |
| AGGTAATTGTATAGGGAGAC+AGG | - | chr8.1:32140604-32140623 | None:intergenic | 40.0% | |
| ATCTTGGACCATAGCTCTTT+GGG | - | chr8.1:32140454-32140473 | None:intergenic | 40.0% | |
| CAATCCCCAACATTAATCCA+AGG | - | chr8.1:32140937-32140956 | None:intergenic | 40.0% | |
| CACCTTCTTATACCAAAGGT+TGG | + | chr8.1:32140692-32140711 | MS.gene44191:CDS | 40.0% | |
| CACTTTGATCAACCAACCTT+TGG | - | chr8.1:32140707-32140726 | None:intergenic | 40.0% | |
| CTCTCCTTGGATTAATGTTG+GGG | + | chr8.1:32140930-32140949 | MS.gene44191:CDS | 40.0% | |
| GAACTGTTGTTACTGTCAAG+AGG | - | chr8.1:32140796-32140815 | None:intergenic | 40.0% | |
| GAAGGAGAATTTGGAAGAGA+GGG | - | chr8.1:32140553-32140572 | None:intergenic | 40.0% | |
| GGACTCAACAAACTCTTCAA+AGG | - | chr8.1:32140748-32140767 | None:intergenic | 40.0% | |
| GGTAATTGTATAGGGAGACA+GGG | - | chr8.1:32140603-32140622 | None:intergenic | 40.0% | |
| TAATGCTCGTATGTACAGCT+TGG | + | chr8.1:32141452-32141471 | MS.gene44191:CDS | 40.0% | |
| TATCTTGGACCATAGCTCTT+TGG | - | chr8.1:32140455-32140474 | None:intergenic | 40.0% | |
| TTGTTTCCAAACCCACACAA+AGG | - | chr8.1:32141156-32141175 | None:intergenic | 40.0% | |
| ! | ATGGTGCGAGGTATTTGTAA+AGG | - | chr8.1:32140580-32140599 | None:intergenic | 40.0% |
| ! | CAAAGGTTGGTTGATCAAAG+TGG | + | chr8.1:32140705-32140724 | MS.gene44191:CDS | 40.0% |
| ! | CAATTTTCACCTCCTTTGTG+TGG | + | chr8.1:32141141-32141160 | MS.gene44191:CDS | 40.0% |
| ! | CACAAAGTCTTGTCAATGCA+AGG | - | chr8.1:32141510-32141529 | None:intergenic | 40.0% |
| ! | CTTGCATTGACAAGACTTTG+TGG | + | chr8.1:32141508-32141527 | MS.gene44191:CDS | 40.0% |
| ! | GATAATTTGCAGTCGGAGAT+AGG | - | chr8.1:32140769-32140788 | None:intergenic | 40.0% |
| !! | GCTTGTTGTATCTTTCAGGA+AGG | + | chr8.1:32140961-32140980 | MS.gene44191:CDS | 40.0% |
| !! | GTATAAGAAGGTGCTAAAGG+TGG | - | chr8.1:32140685-32140704 | None:intergenic | 40.0% |
| ATGTTGGAAGACGCCATGAT+CGG | + | chr8.1:32141253-32141272 | MS.gene44191:CDS | 45.0% | |
| ATTGTATAGGGAGACAGGGA+TGG | - | chr8.1:32140599-32140618 | None:intergenic | 45.0% | |
| ATTTGGAAGAGAGGGAGGAA+TGG | - | chr8.1:32140545-32140564 | None:intergenic | 45.0% | |
| CTTTGTGGTCTCATTCACGT+TGG | + | chr8.1:32141523-32141542 | MS.gene44191:CDS | 45.0% | |
| GGATGAAGAATGGGGGAAAT+GGG | + | chr8.1:32141311-32141330 | MS.gene44191:CDS | 45.0% | |
| TCTTGGACCATAGCTCTTTG+GGG | - | chr8.1:32140453-32140472 | None:intergenic | 45.0% | |
| TGGATGAAGAATGGGGGAAA+TGG | + | chr8.1:32141310-32141329 | MS.gene44191:CDS | 45.0% | |
| TGTCCTATCCTAACTCTCCT+TGG | + | chr8.1:32140917-32140936 | MS.gene44191:CDS | 45.0% | |
| TTTCCAAACCCACACAAAGG+AGG | - | chr8.1:32141153-32141172 | None:intergenic | 45.0% | |
| CTCATCTCCCCAAAGAGCTA+TGG | + | chr8.1:32140443-32140462 | MS.gene44191:CDS | 50.0% | |
| GGAGAATTTGGAAGAGAGGG+AGG | - | chr8.1:32140550-32140569 | None:intergenic | 50.0% | |
| TCACCTCCTTTGTGTGGGTT+TGG | + | chr8.1:32141147-32141166 | MS.gene44191:CDS | 50.0% | |
| TGTTGGAAGACGCCATGATC+GGG | + | chr8.1:32141254-32141273 | MS.gene44191:CDS | 50.0% | |
| AAGACGCCATGATCGGGACG+CGG | + | chr8.1:32141260-32141279 | MS.gene44191:CDS | 60.0% | |
| AGGGAGACAGGGATGGTGCG+AGG | - | chr8.1:32140592-32140611 | None:intergenic | 65.0% | |
| CCACAACCGCGTCCCGATCA+TGG | - | chr8.1:32141269-32141288 | None:intergenic | 65.0% | |
| CCATGATCGGGACGCGGTTG+TGG | + | chr8.1:32141266-32141285 | MS.gene44191:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 32140421 | 32141557 | 32140421 | ID=MS.gene44191 |
| chr8.1 | mRNA | 32140421 | 32141557 | 32140421 | ID=MS.gene44191.t1;Parent=MS.gene44191 |
| chr8.1 | exon | 32140421 | 32141557 | 32140421 | ID=MS.gene44191.t1.exon1;Parent=MS.gene44191.t1 |
| chr8.1 | CDS | 32140421 | 32141557 | 32140421 | ID=cds.MS.gene44191.t1;Parent=MS.gene44191.t1 |
| Gene Sequence |
| Protein sequence |
