AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97013


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97013.t1 MTR_7g096720 97.120 382 11 0 1 382 1 382 0.0 772
MS.gene97013.t1 MTR_4g088925 66.022 362 109 3 21 382 34 381 3.97e-156 444
MS.gene97013.t1 MTR_8g085760 51.786 392 165 9 2 381 4 383 3.37e-125 366
MS.gene97013.t1 MTR_6g092570 43.548 372 167 15 16 378 11 348 3.48e-69 222
MS.gene97013.t1 MTR_5g079830 41.193 352 177 10 16 363 9 334 4.78e-63 207
MS.gene97013.t1 MTR_8g074610 36.554 383 182 17 6 359 20 370 4.32e-50 173
MS.gene97013.t1 MTR_2g041510 35.772 369 182 12 15 359 8 345 4.04e-46 162
MS.gene97013.t1 MTR_2g041510 35.772 369 182 12 15 359 28 365 6.16e-46 162
MS.gene97013.t1 MTR_8g074640 32.440 373 194 11 15 359 8 350 1.78e-45 160
MS.gene97013.t1 MTR_4g125090 38.339 313 139 12 16 325 48 309 2.43e-44 156
MS.gene97013.t1 MTR_7g096760 69.474 95 27 2 228 321 1 94 2.22e-35 127
MS.gene97013.t1 MTR_3g029650 25.858 379 215 11 15 378 7 334 3.23e-17 84.0
MS.gene97013.t1 MTR_3g029650 21.429 378 234 12 7 352 366 712 2.67e-12 68.9
MS.gene97013.t1 MTR_3g029800 23.513 353 208 12 15 348 6 315 1.57e-14 74.7
MS.gene97013.t1 MTR_6g018400 23.056 360 203 13 15 346 7 320 3.49e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97013.t1 AT2G26160 29.714 350 216 11 15 360 7 330 3.90e-34 129
MS.gene97013.t1 AT1G65770 28.571 371 196 11 15 359 5 332 6.06e-34 129
MS.gene97013.t1 AT1G44080 29.830 352 199 9 15 359 7 317 1.88e-31 122
MS.gene97013.t1 AT4G35733 27.606 355 218 8 15 359 8 333 3.10e-28 114
MS.gene97013.t1 AT3G25750 27.095 358 202 12 15 360 7 317 1.35e-23 100
MS.gene97013.t1 AT3G25750 26.722 363 211 13 15 360 7 331 8.34e-23 99.0
MS.gene97013.t1 AT2G17030 24.590 366 216 11 15 348 5 342 3.89e-21 94.7
MS.gene97013.t1 AT1G65740 24.731 372 221 11 15 359 5 344 1.23e-20 92.8
MS.gene97013.t1 AT1G65760 26.146 371 195 12 9 348 1 323 2.08e-20 92.0
MS.gene97013.t1 AT2G17036 24.047 341 221 12 15 337 5 325 1.73e-14 74.7
MS.gene97013.t1 AT2G17036 24.047 341 221 12 15 337 4 324 1.81e-14 74.7
MS.gene97013.t1 AT5G60060 26.136 352 207 17 4 333 1 321 4.74e-12 67.4

Find 63 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACCTGCTGACAAATTT+TGG 0.080030 7.4:+12838927 MS.gene97013:CDS
ACTTTATGTTGTGGATAAAA+TGG 0.170692 7.4:+12838499 MS.gene97013:CDS
GAGTTGACTGTTTCCAATAT+TGG 0.239268 7.4:+12838398 MS.gene97013:CDS
GTGCTGAAAATAGGCATATA+AGG 0.260640 7.4:+12838591 MS.gene97013:CDS
TCCGTAGTGTTTGTAGATTT+TGG 0.264838 7.4:+12837949 MS.gene97013:CDS
AGTCATCATGAAGATAAAAT+TGG 0.275568 7.4:+12838266 MS.gene97013:CDS
TGTACCATTGTGCTGAAAAT+AGG 0.283196 7.4:+12838582 MS.gene97013:CDS
GAGATCAAAGAGATAAAGTT+TGG 0.285701 7.4:-12838147 None:intergenic
TGGTTCAATGCGGTATATTT+TGG 0.305635 7.4:-12838045 None:intergenic
GGGTGAGGTTTCGTTTGTTT+TGG 0.311852 7.4:+12838790 MS.gene97013:CDS
AAAGGAAAATATCATGTTAA+AGG 0.318603 7.4:+12838424 MS.gene97013:CDS
TTGATCAAGAATGGGGTAAA+TGG 0.326226 7.4:+12838750 MS.gene97013:CDS
ATGGTTAGAAGTAAAAGATT+TGG 0.332561 7.4:+12838769 MS.gene97013:CDS
CAAATCTTCTTGAAGATTTG+TGG 0.347804 7.4:+12837880 MS.gene97013:CDS
TGGTTAGAAGTAAAAGATTT+GGG 0.374795 7.4:+12838770 MS.gene97013:CDS
CAATGCTCTTTCCTTGAAGT+TGG 0.381115 7.4:+12838538 MS.gene97013:CDS
CAAAAGGGGTTACTTCTTGA+AGG 0.384442 7.4:+12838703 MS.gene97013:CDS
TTGTGAGAGGCAGGTGGAGG+AGG 0.384893 7.4:-12837984 None:intergenic
AAATCTTCTTGAAGATTTGT+GGG 0.389413 7.4:+12837881 MS.gene97013:CDS
TTTATAAGCTTGATCAAGAA+TGG 0.392244 7.4:+12838741 MS.gene97013:CDS
CTTTATGTTGTGGATAAAAT+GGG 0.396836 7.4:+12838500 MS.gene97013:CDS
TCAACTTCTTGTTCTAATCA+AGG 0.402165 7.4:+12838089 MS.gene97013:CDS
ATGATGAAAGGTATTACAAA+AGG 0.404208 7.4:+12838687 MS.gene97013:CDS
CCAATCCAAAATTTGTCAGC+AGG 0.420447 7.4:-12838932 None:intergenic
TGATGAAAGGTATTACAAAA+GGG 0.432135 7.4:+12838688 MS.gene97013:CDS
TGAAAATTGAACCAACTTCA+AGG 0.432491 7.4:-12838549 None:intergenic
TTGTTGGCTCGAGATTACTA+TGG 0.437431 7.4:+12838833 MS.gene97013:CDS
TGTTTCCAATATTGGAGAAA+AGG 0.445230 7.4:+12838406 MS.gene97013:CDS
TTATAAGCTTGATCAAGAAT+GGG 0.450966 7.4:+12838742 MS.gene97013:CDS
TGATGATATAATACATTACA+AGG 0.467606 7.4:+12838472 MS.gene97013:CDS
TCTACTTCTATTATGAAAGC+AGG 0.486961 7.4:+12838873 MS.gene97013:CDS
TGCTGAAAATAGGCATATAA+GGG 0.494278 7.4:+12838592 MS.gene97013:CDS
CTTACAAATTAATTGATCAC+TGG 0.508472 7.4:-12838971 None:intergenic
ATCACTGGATGGAACAAAGT+TGG 0.510777 7.4:-12838956 None:intergenic
TGATTAGAACAAGAAGTTGA+AGG 0.514698 7.4:-12838086 None:intergenic
ATCAAAGAGATAAAGTTTGG+AGG 0.516104 7.4:-12838144 None:intergenic
GATTAGAACAAGAAGTTGAA+GGG 0.519595 7.4:-12838085 None:intergenic
ATTTATATATTAATGATGAA+AGG 0.523166 7.4:+12838675 MS.gene97013:CDS
CAAGGGACAACTTTATGTTG+TGG 0.530035 7.4:+12838490 MS.gene97013:CDS
ATGCAAAGGTTGTGAGAGGC+AGG 0.537372 7.4:-12837993 None:intergenic
AAGCAATTAGTAGAGTATGA+TGG 0.538440 7.4:+12838635 MS.gene97013:CDS
CAAATTAATTGATCACTGGA+TGG 0.540924 7.4:-12838967 None:intergenic
TGTTGGCTCGAGATTACTAT+GGG 0.552161 7.4:+12838834 MS.gene97013:CDS
AGGTTGTGAGAGGCAGGTGG+AGG 0.559115 7.4:-12837987 None:intergenic
CTTCTTGTTCTAATCAAGGT+GGG 0.559628 7.4:+12838093 MS.gene97013:CDS
GATGAAAGGTATTACAAAAG+GGG 0.559891 7.4:+12838689 MS.gene97013:CDS
AGAAGTAAAAGATTTGGGTG+AGG 0.564111 7.4:+12838775 MS.gene97013:CDS
GGGTTGTGATCATGTGGTGA+TGG 0.571859 7.4:-12838065 None:intergenic
GATGGATATGAATAAGATAG+TGG 0.582410 7.4:+12837845 MS.gene97013:CDS
GCCAAAATCTACAAACACTA+CGG 0.595515 7.4:-12837950 None:intergenic
CTACTTCTATTATGAAAGCA+GGG 0.600724 7.4:+12838874 MS.gene97013:CDS
ACTTCTTGTTCTAATCAAGG+TGG 0.607280 7.4:+12838092 MS.gene97013:CDS
ATCATGTTAAAGGATGATTG+TGG 0.624512 7.4:+12838434 MS.gene97013:CDS
TATAAGCTTGATCAAGAATG+GGG 0.627945 7.4:+12838743 MS.gene97013:CDS
CAAAGGTTGTGAGAGGCAGG+TGG 0.633368 7.4:-12837990 None:intergenic
GATGATATAATACATTACAA+GGG 0.643392 7.4:+12838473 MS.gene97013:CDS
TAATACGAGAATCCGAGCTG+AGG 0.670321 7.4:+12838175 MS.gene97013:CDS
GTTGAAGGGTTGTGATCATG+TGG 0.682135 7.4:-12838071 None:intergenic
TGGAATGCAAAGGTTGTGAG+AGG 0.687706 7.4:-12837997 None:intergenic
GTATGATGGAGGTCTATATG+TGG 0.691113 7.4:+12838649 MS.gene97013:CDS
CAATTAGTAGAGTATGATGG+AGG 0.693642 7.4:+12838638 MS.gene97013:CDS
AGAGTGTGCTATGAATGCGA+TGG 0.712794 7.4:+12837827 None:intergenic
CATGTGGTGATGGTTCAATG+CGG 0.842243 7.4:-12838055 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATATATTAATGATGAA+AGG + chr7.4:12838675-12838694 MS.gene97013:CDS 10.0%
!! AAAGGAAAATATCATGTTAA+AGG + chr7.4:12838424-12838443 MS.gene97013:CDS 20.0%
!! GATGATATAATACATTACAA+GGG + chr7.4:12838473-12838492 MS.gene97013:CDS 20.0%
!! TGATGATATAATACATTACA+AGG + chr7.4:12838472-12838491 MS.gene97013:CDS 20.0%
!!! AGTAATCTTGTTTTTTATTG+AGG + chr7.4:12838340-12838359 MS.gene97013:CDS 20.0%
!!! AGTAGAAAATAATTTTGATG+TGG - chr7.4:12838020-12838039 None:intergenic 20.0%
!!! GTAATCTTGTTTTTTATTGA+GGG + chr7.4:12838341-12838360 MS.gene97013:CDS 20.0%
! AAATCTTCTTGAAGATTTGT+GGG + chr7.4:12837881-12837900 MS.gene97013:CDS 25.0%
! ACTTTATGTTGTGGATAAAA+TGG + chr7.4:12838499-12838518 MS.gene97013:CDS 25.0%
! AGTCATCATGAAGATAAAAT+TGG + chr7.4:12838266-12838285 MS.gene97013:CDS 25.0%
! ATGGTTAGAAGTAAAAGATT+TGG + chr7.4:12838769-12838788 MS.gene97013:CDS 25.0%
! CTTTATGTTGTGGATAAAAT+GGG + chr7.4:12838500-12838519 MS.gene97013:CDS 25.0%
! TGGTTAGAAGTAAAAGATTT+GGG + chr7.4:12838770-12838789 MS.gene97013:CDS 25.0%
! TTATAAGCTTGATCAAGAAT+GGG + chr7.4:12838742-12838761 MS.gene97013:CDS 25.0%
! TTTATAAGCTTGATCAAGAA+TGG + chr7.4:12838741-12838760 MS.gene97013:CDS 25.0%
!! ATAAAATGGGAACAATTTTC+TGG + chr7.4:12838513-12838532 MS.gene97013:CDS 25.0%
!! ATTTTCCTTTTCTCCAATAT+TGG - chr7.4:12838414-12838433 None:intergenic 25.0%
!! CATCAAATCCAAAACTTTTT+CGG - chr7.4:12838207-12838226 None:intergenic 25.0%
!!! ATGATGAAAGGTATTACAAA+AGG + chr7.4:12838687-12838706 MS.gene97013:CDS 25.0%
!!! TGATGAAAGGTATTACAAAA+GGG + chr7.4:12838688-12838707 MS.gene97013:CDS 25.0%
AAGCAATTAGTAGAGTATGA+TGG + chr7.4:12838635-12838654 MS.gene97013:CDS 30.0%
ATCAAAGAGATAAAGTTTGG+AGG - chr7.4:12838147-12838166 None:intergenic 30.0%
ATCATGTTAAAGGATGATTG+TGG + chr7.4:12838434-12838453 MS.gene97013:CDS 30.0%
CAAATCTTCTTGAAGATTTG+TGG + chr7.4:12837880-12837899 MS.gene97013:CDS 30.0%
CTACTTCTATTATGAAAGCA+GGG + chr7.4:12838874-12838893 MS.gene97013:CDS 30.0%
GAGATCAAAGAGATAAAGTT+TGG - chr7.4:12838150-12838169 None:intergenic 30.0%
GATGGATATGAATAAGATAG+TGG + chr7.4:12837845-12837864 MS.gene97013:CDS 30.0%
GATTAGAACAAGAAGTTGAA+GGG - chr7.4:12838088-12838107 None:intergenic 30.0%
TATAAGCTTGATCAAGAATG+GGG + chr7.4:12838743-12838762 MS.gene97013:CDS 30.0%
TCAACTTCTTGTTCTAATCA+AGG + chr7.4:12838089-12838108 MS.gene97013:CDS 30.0%
TCTACTTCTATTATGAAAGC+AGG + chr7.4:12838873-12838892 MS.gene97013:CDS 30.0%
TGAAAATTGAACCAACTTCA+AGG - chr7.4:12838552-12838571 None:intergenic 30.0%
TGATTAGAACAAGAAGTTGA+AGG - chr7.4:12838089-12838108 None:intergenic 30.0%
TGCTGAAAATAGGCATATAA+GGG + chr7.4:12838592-12838611 MS.gene97013:CDS 30.0%
TGTTTCCAATATTGGAGAAA+AGG + chr7.4:12838406-12838425 MS.gene97013:CDS 30.0%
! AGACACAAATTTTGCATTGT+TGG + chr7.4:12838817-12838836 MS.gene97013:CDS 30.0%
! GGAAAATACCGAAAAAGTTT+TGG + chr7.4:12838196-12838215 MS.gene97013:CDS 30.0%
!! AATTTTGATGTGGAATGCAA+AGG - chr7.4:12838010-12838029 None:intergenic 30.0%
!! GATGAAAGGTATTACAAAAG+GGG + chr7.4:12838689-12838708 MS.gene97013:CDS 30.0%
!!! AATCGTAGTGTTCAGTTTTT+TGG - chr7.4:12837916-12837935 None:intergenic 30.0%
ACTTCTTGTTCTAATCAAGG+TGG + chr7.4:12838092-12838111 MS.gene97013:CDS 35.0%
AGAAGTAAAAGATTTGGGTG+AGG + chr7.4:12838775-12838794 MS.gene97013:CDS 35.0%
CAATTAGTAGAGTATGATGG+AGG + chr7.4:12838638-12838657 MS.gene97013:CDS 35.0%
CTTCTTGTTCTAATCAAGGT+GGG + chr7.4:12838093-12838112 MS.gene97013:CDS 35.0%
GAGTTGACTGTTTCCAATAT+TGG + chr7.4:12838398-12838417 MS.gene97013:CDS 35.0%
GCCAAAATCTACAAACACTA+CGG - chr7.4:12837953-12837972 None:intergenic 35.0%
GTGCTGAAAATAGGCATATA+AGG + chr7.4:12838591-12838610 MS.gene97013:CDS 35.0%
TGTACCATTGTGCTGAAAAT+AGG + chr7.4:12838582-12838601 MS.gene97013:CDS 35.0%
TTGATCAAGAATGGGGTAAA+TGG + chr7.4:12838750-12838769 MS.gene97013:CDS 35.0%
! CTAAACCTGCTGACAAATTT+TGG + chr7.4:12838927-12838946 MS.gene97013:CDS 35.0%
! TATGCCTATTTTCAGCACAA+TGG - chr7.4:12838589-12838608 None:intergenic 35.0%
! TCCGTAGTGTTTGTAGATTT+TGG + chr7.4:12837949-12837968 MS.gene97013:CDS 35.0%
! TGGTTCAATGCGGTATATTT+TGG - chr7.4:12838048-12838067 None:intergenic 35.0%
!!! CAATGCGGTATATTTTGGTT+TGG - chr7.4:12838043-12838062 None:intergenic 35.0%
!!! GCACAATGGTACATGTTTTT+TGG - chr7.4:12838575-12838594 None:intergenic 35.0%
ATCACTGGATGGAACAAAGT+TGG - chr7.4:12838959-12838978 None:intergenic 40.0%
CAAAAGGGGTTACTTCTTGA+AGG + chr7.4:12838703-12838722 MS.gene97013:CDS 40.0%
CAAGGGACAACTTTATGTTG+TGG + chr7.4:12838490-12838509 MS.gene97013:CDS 40.0%
CAATGCTCTTTCCTTGAAGT+TGG + chr7.4:12838538-12838557 MS.gene97013:CDS 40.0%
CCAATCCAAAATTTGTCAGC+AGG - chr7.4:12838935-12838954 None:intergenic 40.0%
! CCTGCTGACAAATTTTGGAT+TGG + chr7.4:12838932-12838951 MS.gene97013:CDS 40.0%
! GTATGATGGAGGTCTATATG+TGG + chr7.4:12838649-12838668 MS.gene97013:CDS 40.0%
! TTTCGGTATTTTCCTCAGCT+CGG - chr7.4:12838190-12838209 None:intergenic 40.0%
! TTTTATTGAGGGTCGTTGCA+TGG + chr7.4:12838352-12838371 MS.gene97013:CDS 40.0%
!! TGTTGGCTCGAGATTACTAT+GGG + chr7.4:12838834-12838853 MS.gene97013:CDS 40.0%
!! TTGTTGGCTCGAGATTACTA+TGG + chr7.4:12838833-12838852 MS.gene97013:CDS 40.0%
!!! CTTCTTGAAGGCTGTTTTTG+TGG + chr7.4:12838715-12838734 MS.gene97013:CDS 40.0%
GTTGAAGGGTTGTGATCATG+TGG - chr7.4:12838074-12838093 None:intergenic 45.0%
TAATACGAGAATCCGAGCTG+AGG + chr7.4:12838175-12838194 MS.gene97013:CDS 45.0%
TGGAATGCAAAGGTTGTGAG+AGG - chr7.4:12838000-12838019 None:intergenic 45.0%
! GGGTGAGGTTTCGTTTGTTT+TGG + chr7.4:12838790-12838809 MS.gene97013:CDS 45.0%
!! CATGTGGTGATGGTTCAATG+CGG - chr7.4:12838058-12838077 None:intergenic 45.0%
ATGCAAAGGTTGTGAGAGGC+AGG - chr7.4:12837996-12838015 None:intergenic 50.0%
GGGTTGTGATCATGTGGTGA+TGG - chr7.4:12838068-12838087 None:intergenic 50.0%
CAAAGGTTGTGAGAGGCAGG+TGG - chr7.4:12837993-12838012 None:intergenic 55.0%
AGGTTGTGAGAGGCAGGTGG+AGG - chr7.4:12837990-12838009 None:intergenic 60.0%
TTGTGAGAGGCAGGTGGAGG+AGG - chr7.4:12837987-12838006 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 12837837 12838985 12837837 ID=MS.gene97013
chr7.4 mRNA 12837837 12838985 12837837 ID=MS.gene97013.t1;Parent=MS.gene97013
chr7.4 exon 12837837 12838985 12837837 ID=MS.gene97013.t1.exon1;Parent=MS.gene97013.t1
chr7.4 CDS 12837837 12838985 12837837 ID=cds.MS.gene97013.t1;Parent=MS.gene97013.t1
Gene Sequence

>MS.gene97013

ATGAATGCGATGGATATGAATAAGATAGTGGAGTGTTCAAACTCAAATCTTCTTGAAGATTTGTGGGCAATTATTTCCAAAAAACTGAACACTACGATTGATGTTGTTCGATTCCGTAGTGTTTGTAGATTTTGGCGCTCTCTTCTTCCTCCTCCACCTGCCTCTCACAACCTTTGCATTCCACATCAAAATTATTTTCTACTCCAAACCAAAATATACCGCATTGAACCATCACCACATGATCACAACCCTTCAACTTCTTGTTCTAATCAAGGTGGGATCATTAAAGTTTTTCAAAATTCAAATTCCTCCAAACTTTATCTCTTTGATCTCTTCACTAATACGAGAATCCGAGCTGAGGAAAATACCGAAAAAGTTTTGGATTTGATGAACTTTCGAGTTGTTGAGTTGTCTAAAGTATATACTCGAAGTCATCATGAAGATAAAATTGGTTTTAAGTGTGCATCTCGTAATAATGTTTGCAATGTTTGCAATATTTGCAAAGTAATCTTGTTTTTTATTGAGGGTCGTTGCATGGTCTTTGCACTGCATAATGATAAAGAGTTGACTGTTTCCAATATTGGAGAAAAGGAAAATATCATGTTAAAGGATGATTGTGGAGAAAACAAATATTTTGATGATATAATACATTACAAGGGACAACTTTATGTTGTGGATAAAATGGGAACAATTTTCTGGATCAATGCTCTTTCCTTGAAGTTGGTTCAATTTTCACCAAAAAACATGTACCATTGTGCTGAAAATAGGCATATAAGGGTGAATTCTAACATAAACAAGAAGCAATTAGTAGAGTATGATGGAGGTCTATATGTGGTTGATTTATATATTAATGATGAAAGGTATTACAAAAGGGGTTACTTCTTGAAGGCTGTTTTTGTGGAAGTTTATAAGCTTGATCAAGAATGGGGTAAATGGTTAGAAGTAAAAGATTTGGGTGAGGTTTCGTTTGTTTTGGATAAAGACACAAATTTTGCATTGTTGGCTCGAGATTACTATGGGTGTGAAGAGAACTGCATCTACTTCTATTATGAAAGCAGGGCATCTTGTTTCAACTTGAAGAGTTTAGAGTCTAAACCTGCTGACAAATTTTGGATTGGTCCAACTTTGTTCCATCCAGTGATCAATTAA

Protein sequence

>MS.gene97013.t1

MNAMDMNKIVECSNSNLLEDLWAIISKKLNTTIDVVRFRSVCRFWRSLLPPPPASHNLCIPHQNYFLLQTKIYRIEPSPHDHNPSTSCSNQGGIIKVFQNSNSSKLYLFDLFTNTRIRAEENTEKVLDLMNFRVVELSKVYTRSHHEDKIGFKCASRNNVCNVCNICKVILFFIEGRCMVFALHNDKELTVSNIGEKENIMLKDDCGENKYFDDIIHYKGQLYVVDKMGTIFWINALSLKLVQFSPKNMYHCAENRHIRVNSNINKKQLVEYDGGLYVVDLYINDERYYKRGYFLKAVFVEVYKLDQEWGKWLEVKDLGEVSFVLDKDTNFALLARDYYGCEENCIYFYYESRASCFNLKSLESKPADKFWIGPTLFHPVIN