AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64423

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64423.t1 MTR_2g041510 89.474 399 32 4 1 396 1 392 0.0 699
MS.gene64423.t1 MTR_2g041510 89.974 379 29 3 20 396 1 372 0.0 687
MS.gene64423.t1 MTR_8g074610 79.397 398 79 3 1 396 1 397 0.0 633
MS.gene64423.t1 MTR_8g074640 74.603 378 94 2 20 396 1 377 0.0 572
MS.gene64423.t1 MTR_8g085760 34.596 396 196 14 21 382 9 375 1.16e-53 182
MS.gene64423.t1 MTR_6g092570 35.027 374 197 10 21 382 4 343 1.15e-50 174
MS.gene64423.t1 MTR_7g096720 35.389 373 183 13 27 369 15 359 3.42e-47 166
MS.gene64423.t1 MTR_5g079830 32.432 370 200 8 20 374 1 335 1.91e-46 164
MS.gene64423.t1 MTR_4g088925 33.690 374 189 12 33 382 34 372 1.27e-45 161
MS.gene64423.t1 MTR_3g030050 31.100 418 229 12 18 388 9 414 3.53e-41 150
MS.gene64423.t1 MTR_6g018390 31.362 389 210 16 24 388 4 359 5.15e-33 127
MS.gene64423.t1 MTR_2g047180 27.979 386 228 13 24 388 3 359 3.26e-32 125
MS.gene64423.t1 MTR_1g026850 30.239 377 205 15 20 373 1 342 9.47e-32 124
MS.gene64423.t1 MTR_6g027100 29.925 401 210 17 24 388 4 369 8.88e-31 122
MS.gene64423.t1 MTR_1g007260 27.525 396 226 15 7 374 5 367 3.86e-30 120
MS.gene64423.t1 MTR_3g030360 28.426 394 204 14 22 388 2 344 1.30e-28 115
MS.gene64423.t1 MTR_3g029650 28.652 356 188 11 24 358 4 314 9.37e-28 115
MS.gene64423.t1 MTR_3g029650 26.131 398 229 14 16 375 364 734 2.49e-26 111
MS.gene64423.t1 MTR_3g029640 28.084 381 206 12 24 380 3 339 1.04e-27 112
MS.gene64423.t1 MTR_6g027040 27.889 398 222 16 24 388 4 369 1.23e-27 112
MS.gene64423.t1 MTR_3g058460 32.759 348 193 17 25 360 15 333 2.72e-27 112
MS.gene64423.t1 MTR_3g030380 28.455 369 218 11 23 377 3 339 7.00e-27 110
MS.gene64423.t1 MTR_6g018360 28.261 368 197 14 24 360 4 335 5.61e-26 108
MS.gene64423.t1 MTR_6g018400 27.720 386 209 13 22 380 2 344 1.35e-25 107
MS.gene64423.t1 MTR_3g029620 27.487 382 217 13 24 380 2 348 4.05e-25 105
MS.gene64423.t1 MTR_1g016570 27.411 394 212 15 16 388 13 353 6.38e-25 105
MS.gene64423.t1 MTR_3g030480 27.838 370 215 12 24 377 4 337 6.91e-25 104
MS.gene64423.t1 MTR_3g030355 27.500 400 233 16 20 381 1 381 1.24e-24 104
MS.gene64423.t1 MTR_3g030440 27.005 374 221 12 20 377 1 338 1.49e-23 100
MS.gene64423.t1 MTR_4g125090 28.221 326 168 10 21 336 41 310 1.74e-23 100
MS.gene64423.t1 MTR_3g114970 25.676 370 230 13 24 381 3 339 3.70e-23 99.8
MS.gene64423.t1 MTR_1g049170 26.290 407 232 17 20 388 1 377 4.48e-23 100
MS.gene64423.t1 MTR_3g030080 27.557 352 207 12 25 360 7 326 5.12e-23 99.8
MS.gene64423.t1 MTR_3g029920 29.032 403 209 18 18 388 2 359 8.50e-23 99.4
MS.gene64423.t1 MTR_3g091990 25.436 401 236 15 23 387 5 378 9.38e-22 96.7
MS.gene64423.t1 MTR_3g029840 26.648 364 192 14 24 360 4 319 1.61e-21 95.1
MS.gene64423.t1 MTR_3g030560 26.425 386 221 14 23 377 2 355 2.86e-21 94.7
MS.gene64423.t1 MTR_3g114990 25.833 360 225 11 24 363 22 359 3.94e-21 94.7
MS.gene64423.t1 MTR_3g029950 27.665 394 201 19 24 383 4 347 6.92e-21 93.6
MS.gene64423.t1 MTR_3g037710 32.381 210 117 6 180 375 45 243 7.35e-21 92.0
MS.gene64423.t1 MTR_3g029670 26.214 412 216 17 24 385 4 377 1.91e-20 92.4
MS.gene64423.t1 MTR_6g018315 25.897 390 225 13 37 391 2 362 3.36e-20 92.0
MS.gene64423.t1 MTR_3g029750 25.587 383 214 13 24 369 4 352 3.80e-19 88.6
MS.gene64423.t1 MTR_3g092000 24.607 382 213 14 23 360 5 355 4.59e-19 88.6
MS.gene64423.t1 MTR_3g047980 30.504 377 196 18 25 388 30 353 1.82e-16 80.5
MS.gene64423.t1 MTR_3g029820 26.980 404 176 17 18 393 2 314 5.05e-16 79.0
MS.gene64423.t1 MTR_5g042230 36.184 152 83 5 209 358 13 152 5.49e-14 70.9
MS.gene64423.t1 MTR_1g016540 32.240 183 94 7 224 388 2 172 1.01e-13 69.7
MS.gene64423.t1 MTR_3g029900 32.370 173 81 6 217 386 297 436 1.36e-13 72.4
MS.gene64423.t1 MTR_6g018370 35.659 129 73 4 209 336 132 251 4.81e-13 68.9
MS.gene64423.t1 MTR_1g007250 34.177 158 81 6 226 374 4 147 4.66e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64423.t1 AT1G65770 29.508 366 221 11 24 378 2 341 2.78e-39 144
MS.gene64423.t1 AT4G35733 27.979 386 205 11 20 377 1 341 1.36e-37 139
MS.gene64423.t1 AT2G17030 30.933 375 207 15 24 371 2 351 2.25e-37 140
MS.gene64423.t1 AT2G26160 32.033 359 192 15 22 365 2 323 1.33e-34 130
MS.gene64423.t1 AT3G25750 28.238 386 199 13 22 375 2 341 5.21e-33 127
MS.gene64423.t1 AT3G25750 29.111 371 201 13 22 375 2 327 7.45e-32 124
MS.gene64423.t1 AT1G44080 29.620 368 207 11 23 380 3 328 2.50e-31 122
MS.gene64423.t1 AT2G17036 27.704 379 230 13 24 379 1 358 1.03e-29 119
MS.gene64423.t1 AT2G17036 27.704 379 230 13 24 379 2 359 1.09e-29 119
MS.gene64423.t1 AT1G65740 30.000 370 224 14 24 377 2 352 1.14e-28 115
MS.gene64423.t1 AT1G65760 28.378 370 216 14 25 377 5 342 1.24e-25 107
MS.gene64423.t1 AT2G17690 27.896 423 215 16 25 377 3 405 3.07e-24 103
MS.gene64423.t1 AT2G16290 26.355 406 229 16 24 382 2 384 1.59e-21 95.9
MS.gene64423.t1 AT5G60060 28.610 374 207 20 15 363 2 340 2.87e-19 89.0
MS.gene64423.t1 AT2G24250 27.298 359 184 19 25 358 15 321 5.44e-17 82.4
MS.gene64423.t1 AT2G24250 27.298 359 184 19 25 358 15 321 5.44e-17 82.4
MS.gene64423.t1 AT5G24040 24.665 373 197 14 24 360 2 326 2.03e-15 77.4
MS.gene64423.t1 AT1G57906 31.515 165 76 5 215 347 67 226 5.66e-14 71.2

Find 77 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGAATTGCATCTACTTTA+AGG 0.156851 2.4:+48638603 MS.gene64423:CDS
TATAAAATTGAAAAGATTAA+AGG 0.167135 2.4:+48638046 MS.gene64423:CDS
TAATGTTCGTATGTATAATT+TGG 0.171010 2.4:+48638633 MS.gene64423:CDS
GTTTCGGCTAAAGAATTAAT+TGG 0.188705 2.4:+48638574 MS.gene64423:CDS
TCACCTCCTTTGTGTGGTTT+TGG 0.214421 2.4:+48638325 MS.gene64423:CDS
AAAGGAAAAGAAGGAGAATT+TGG 0.264948 2.4:-48637743 None:intergenic
TATGGAACATGTTGGATTAT+AGG 0.282617 2.4:+48638002 MS.gene64423:CDS
AGAAGAAGAAGGTGCTAAAC+TGG 0.284874 2.4:-48637855 None:intergenic
TTCCAAAGGTTGGTTGATTA+AGG 0.293669 2.4:+48637883 MS.gene64423:CDS
ATGGAACATGTTGGATTATA+GGG 0.306390 2.4:+48638003 MS.gene64423:CDS
ACAACACTTCCCTGATGATA+TGG 0.323704 2.4:+48637984 MS.gene64423:CDS
AGTTGGTTGAATAAGATAAA+TGG 0.329748 2.4:-48637816 None:intergenic
CTTCTTCTTCTTCCAAAGGT+TGG 0.349032 2.4:+48637873 MS.gene64423:CDS
ATCTTTCAAGAAGATATAAT+AGG 0.368485 2.4:+48638148 MS.gene64423:CDS
ATAAATGGGGAACTATTTCA+TGG 0.373052 2.4:+48638269 MS.gene64423:CDS
TAGTTGTAACTTCTCCGTTT+CGG 0.375415 2.4:+48638558 MS.gene64423:CDS
TACCTTAATCAACCAACCTT+TGG 0.382386 2.4:-48637885 None:intergenic
TAAAACATGGTGATGAGAAA+TGG 0.387502 2.4:+48638176 MS.gene64423:CDS
ACACTACTCCAAGGAGAGTT+AGG 0.387864 2.4:-48638103 None:intergenic
CCACAACCGCGTATCGATCT+TGG 0.394717 2.4:-48638447 None:intergenic
CTCAACTCCCTACAGAACTA+TGG 0.406799 2.4:+48637627 MS.gene64423:CDS
ATTTATCAGTAACATAAAAC+TGG 0.411203 2.4:-48638252 None:intergenic
AAAGAATTAATTGGATATCA+AGG 0.411865 2.4:+48638583 MS.gene64423:CDS
CGATGATATTATTGTGTTCA+AGG 0.411971 2.4:+48638228 MS.gene64423:CDS
GAATAAGATAAATGGTTGAT+TGG 0.414572 2.4:-48637808 None:intergenic
TGTTCTATCCTAACTCTCCT+TGG 0.425110 2.4:+48638095 MS.gene64423:CDS
CTTGCATCGACAACACTTTG+TGG 0.427663 2.4:+48638689 MS.gene64423:CDS
CTATAAGTTGGATGAAGAAT+GGG 0.428624 2.4:+48638483 MS.gene64423:CDS
CCAACATGTTCCATATCATC+AGG 0.435629 2.4:-48637994 None:intergenic
TTGGTTGAGGTAGTTGTTTG+TGG 0.440994 2.4:-48637789 None:intergenic
AACATTTGGTGGAATCTTGT+GGG 0.442266 2.4:+48638356 MS.gene64423:CDS
ACAGAACTATGGCCTAAGAT+AGG 0.442853 2.4:+48637638 MS.gene64423:CDS
TCTATAAGTTGGATGAAGAA+TGG 0.449108 2.4:+48638482 MS.gene64423:CDS
AATTAATTCTTTAGCCGAAA+CGG 0.462912 2.4:-48638572 None:intergenic
CCAAGATCGATACGCGGTTG+TGG 0.468738 2.4:+48638447 MS.gene64423:CDS
GATAAATGGTTGATTGGTTG+AGG 0.469906 2.4:-48637802 None:intergenic
AATTATCATATCCTCTTAGC+AGG 0.479331 2.4:+48637960 MS.gene64423:CDS
AAACATTTGGTGGAATCTTG+TGG 0.483180 2.4:+48638355 MS.gene64423:CDS
ATCTTAGGCCATAGTTCTGT+AGG 0.489468 2.4:-48637635 None:intergenic
GGAGAATTTGGAAGAGAATG+AGG 0.493609 2.4:-48637731 None:intergenic
AGAAGGTGCTAAACTGGAGT+TGG 0.502318 2.4:-48637849 None:intergenic
CACCTCGGCAGAGATGTTAG+AGG 0.525382 2.4:+48638719 MS.gene64423:CDS
GGTATTTGCAAAGGAAAAGA+AGG 0.529929 2.4:-48637752 None:intergenic
TAGAACACAACTTTCTTCAC+AGG 0.530312 2.4:-48638079 None:intergenic
GTGTTTCAAAGTCTATAAGT+TGG 0.530775 2.4:+48638471 MS.gene64423:CDS
TTTGTGGAGATGTTGATGCC+AGG 0.530799 2.4:-48637773 None:intergenic
ACAACTAGGGCTCAAAATGA+AGG 0.531781 2.4:-48638542 None:intergenic
CCAATCTACTTTCTCATCCA+TGG 0.539558 2.4:-48637603 None:intergenic
CTCTCCTTGGAGTAGTGTTG+AGG 0.544030 2.4:+48638108 MS.gene64423:CDS
ATTTGGAAGAGAATGAGGAA+TGG 0.545115 2.4:-48637726 None:intergenic
ATCTGATTCAACTTCAGCCA+TGG 0.552026 2.4:+48637586 MS.gene64423:CDS
TGGAAACAAGAAACATTTGG+TGG 0.554210 2.4:+48638345 MS.gene64423:CDS
CACAAAGTGTTGTCGATGCA+AGG 0.554548 2.4:-48638688 None:intergenic
GATGATATTATTGTGTTCAA+GGG 0.555110 2.4:+48638229 MS.gene64423:CDS
CCTGATGATATGGAACATGT+TGG 0.555978 2.4:+48637994 MS.gene64423:CDS
AACGTGAGAAGGAACTATGT+TGG 0.556354 2.4:+48638415 MS.gene64423:CDS
AAGAATTAATTGGATATCAA+GGG 0.561141 2.4:+48638584 MS.gene64423:CDS
CCATGGATGAGAAAGTAGAT+TGG 0.566456 2.4:+48637603 MS.gene64423:CDS
GAAGTGTTGTTCCTGCTAAG+AGG 0.569462 2.4:-48637971 None:intergenic
TCTTAGGCCATAGTTCTGTA+GGG 0.578657 2.4:-48637634 None:intergenic
TATAAGTTGGATGAAGAATG+GGG 0.579436 2.4:+48638484 MS.gene64423:CDS
GAAACGGAGAAGTTACAACT+AGG 0.595840 2.4:-48638556 None:intergenic
ATAGGATTCAACAAACTCAA+AGG 0.597227 2.4:-48637926 None:intergenic
GTCCTCTAACATCTCTGCCG+AGG 0.609853 2.4:-48638721 None:intergenic
TTGATGCCAGGTATTTGCAA+AGG 0.610176 2.4:-48637761 None:intergenic
ACGAAAGCGAAAACGTGAGA+AGG 0.630900 2.4:+48638404 MS.gene64423:CDS
CTTTGTGGTGTCATGCACCT+CGG 0.641274 2.4:+48638704 MS.gene64423:CDS
GAGTTGGAACTTGCATCAGT+TGG 0.641493 2.4:-48637833 None:intergenic
CAACATGTTCCATATCATCA+GGG 0.642106 2.4:-48637993 None:intergenic
CAATCCTCAACACTACTCCA+AGG 0.642424 2.4:-48638112 None:intergenic
TTTCCAAAACCACACAAAGG+AGG 0.646363 2.4:-48638328 None:intergenic
AAACGGAGAAGTTACAACTA+GGG 0.649640 2.4:-48638555 None:intergenic
ATAATAGGATTCATAAAACA+TGG 0.653150 2.4:+48638163 MS.gene64423:CDS
GATGCAAGGATTAAAATCAA+CGG 0.664976 2.4:-48638674 None:intergenic
TTGTTTCCAAAACCACACAA+AGG 0.670548 2.4:-48638331 None:intergenic
ATAAGTTGGATGAAGAATGG+GGG 0.674708 2.4:+48638485 MS.gene64423:CDS
AAGACACCAAGATCGATACG+CGG 0.821666 2.4:+48638441 MS.gene64423:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAATTGAAAAGATTAA+AGG + chr2.4:48638046-48638065 MS.gene64423:CDS 10.0%
!! AAAGAATTAATTGGATATCA+AGG + chr2.4:48638583-48638602 MS.gene64423:CDS 20.0%
!! AAGAATTAATTGGATATCAA+GGG + chr2.4:48638584-48638603 MS.gene64423:CDS 20.0%
!! ATAATAGGATTCATAAAACA+TGG + chr2.4:48638163-48638182 MS.gene64423:CDS 20.0%
!! ATCTTTCAAGAAGATATAAT+AGG + chr2.4:48638148-48638167 MS.gene64423:CDS 20.0%
!! ATTTATCAGTAACATAAAAC+TGG - chr2.4:48638255-48638274 None:intergenic 20.0%
!! TAATGTTCGTATGTATAATT+TGG + chr2.4:48638633-48638652 MS.gene64423:CDS 20.0%
!!! AGGATATGATAATTTTCTTT+CGG - chr2.4:48637954-48637973 None:intergenic 20.0%
!!! AGTTTTATGTTACTGATAAA+TGG + chr2.4:48638254-48638273 MS.gene64423:CDS 20.0%
!!! GTTTTATGTTACTGATAAAT+GGG + chr2.4:48638255-48638274 MS.gene64423:CDS 20.0%
!!! TTTTATGTTACTGATAAATG+GGG + chr2.4:48638256-48638275 MS.gene64423:CDS 20.0%
! AATTAATTCTTTAGCCGAAA+CGG - chr2.4:48638575-48638594 None:intergenic 25.0%
! AGTTGGTTGAATAAGATAAA+TGG - chr2.4:48637819-48637838 None:intergenic 25.0%
! GAATAAGATAAATGGTTGAT+TGG - chr2.4:48637811-48637830 None:intergenic 25.0%
! GATGATATTATTGTGTTCAA+GGG + chr2.4:48638229-48638248 MS.gene64423:CDS 25.0%
!! TCAAGATATTTTCCTATCTT+AGG - chr2.4:48637653-48637672 None:intergenic 25.0%
!!! TTTTGGAAACAAGAAACATT+TGG + chr2.4:48638342-48638361 MS.gene64423:CDS 25.0%
AAAGGAAAAGAAGGAGAATT+TGG - chr2.4:48637746-48637765 None:intergenic 30.0%
AATTATCATATCCTCTTAGC+AGG + chr2.4:48637960-48637979 MS.gene64423:CDS 30.0%
ATAAATGGGGAACTATTTCA+TGG + chr2.4:48638269-48638288 MS.gene64423:CDS 30.0%
ATAGGATTCAACAAACTCAA+AGG - chr2.4:48637929-48637948 None:intergenic 30.0%
ATGGAACATGTTGGATTATA+GGG + chr2.4:48638003-48638022 MS.gene64423:CDS 30.0%
CGATGATATTATTGTGTTCA+AGG + chr2.4:48638228-48638247 MS.gene64423:CDS 30.0%
GATGCAAGGATTAAAATCAA+CGG - chr2.4:48638677-48638696 None:intergenic 30.0%
GTGTTTCAAAGTCTATAAGT+TGG + chr2.4:48638471-48638490 MS.gene64423:CDS 30.0%
GTTTCGGCTAAAGAATTAAT+TGG + chr2.4:48638574-48638593 MS.gene64423:CDS 30.0%
TATGGAACATGTTGGATTAT+AGG + chr2.4:48638002-48638021 MS.gene64423:CDS 30.0%
! CTATAAGTTGGATGAAGAAT+GGG + chr2.4:48638483-48638502 MS.gene64423:CDS 30.0%
! GATAATTTTCTTTCGGTGAT+AGG - chr2.4:48637947-48637966 None:intergenic 30.0%
! TAAAACATGGTGATGAGAAA+TGG + chr2.4:48638176-48638195 MS.gene64423:CDS 30.0%
! TATAAGTTGGATGAAGAATG+GGG + chr2.4:48638484-48638503 MS.gene64423:CDS 30.0%
! TCTATAAGTTGGATGAAGAA+TGG + chr2.4:48638482-48638501 MS.gene64423:CDS 30.0%
AAACGGAGAAGTTACAACTA+GGG - chr2.4:48638558-48638577 None:intergenic 35.0%
AGGGAATTGCATCTACTTTA+AGG + chr2.4:48638603-48638622 MS.gene64423:CDS 35.0%
ATTTGGAAGAGAATGAGGAA+TGG - chr2.4:48637729-48637748 None:intergenic 35.0%
CAACATGTTCCATATCATCA+GGG - chr2.4:48637996-48638015 None:intergenic 35.0%
GGTATTTGCAAAGGAAAAGA+AGG - chr2.4:48637755-48637774 None:intergenic 35.0%
TACCTTAATCAACCAACCTT+TGG - chr2.4:48637888-48637907 None:intergenic 35.0%
TAGAACACAACTTTCTTCAC+AGG - chr2.4:48638082-48638101 None:intergenic 35.0%
TAGTTGTAACTTCTCCGTTT+CGG + chr2.4:48638558-48638577 MS.gene64423:CDS 35.0%
TCTTCTTCTTCTTCTTCCAA+AGG + chr2.4:48637869-48637888 MS.gene64423:CDS 35.0%
TGGAAACAAGAAACATTTGG+TGG + chr2.4:48638345-48638364 MS.gene64423:CDS 35.0%
TTGGAAGAAGAAGAAGAAGA+AGG - chr2.4:48637869-48637888 None:intergenic 35.0%
TTGTTTCCAAAACCACACAA+AGG - chr2.4:48638334-48638353 None:intergenic 35.0%
! AAACATTTGGTGGAATCTTG+TGG + chr2.4:48638355-48638374 MS.gene64423:CDS 35.0%
! AACATTTGGTGGAATCTTGT+GGG + chr2.4:48638356-48638375 MS.gene64423:CDS 35.0%
! ATAAGTTGGATGAAGAATGG+GGG + chr2.4:48638485-48638504 MS.gene64423:CDS 35.0%
! GATAAATGGTTGATTGGTTG+AGG - chr2.4:48637805-48637824 None:intergenic 35.0%
! TCTTTTCCTTTGCAAATACC+TGG + chr2.4:48637755-48637774 MS.gene64423:CDS 35.0%
! TTCCAAAGGTTGGTTGATTA+AGG + chr2.4:48637883-48637902 MS.gene64423:CDS 35.0%
AACGTGAGAAGGAACTATGT+TGG + chr2.4:48638415-48638434 MS.gene64423:CDS 40.0%
ACAACACTTCCCTGATGATA+TGG + chr2.4:48637984-48638003 MS.gene64423:CDS 40.0%
ACAACTAGGGCTCAAAATGA+AGG - chr2.4:48638545-48638564 None:intergenic 40.0%
ACAGAACTATGGCCTAAGAT+AGG + chr2.4:48637638-48637657 MS.gene64423:CDS 40.0%
ATCTGATTCAACTTCAGCCA+TGG + chr2.4:48637586-48637605 MS.gene64423:CDS 40.0%
CCAACATGTTCCATATCATC+AGG - chr2.4:48637997-48638016 None:intergenic 40.0%
CCAATCTACTTTCTCATCCA+TGG - chr2.4:48637606-48637625 None:intergenic 40.0%
CCATGGATGAGAAAGTAGAT+TGG + chr2.4:48637603-48637622 MS.gene64423:CDS 40.0%
CCTGATGATATGGAACATGT+TGG + chr2.4:48637994-48638013 MS.gene64423:CDS 40.0%
CTTCTTCTTCTTCCAAAGGT+TGG + chr2.4:48637873-48637892 MS.gene64423:CDS 40.0%
GAAACGGAGAAGTTACAACT+AGG - chr2.4:48638559-48638578 None:intergenic 40.0%
GGAGAATTTGGAAGAGAATG+AGG - chr2.4:48637734-48637753 None:intergenic 40.0%
TGTTCTATCCTAACTCTCCT+TGG + chr2.4:48638095-48638114 MS.gene64423:CDS 40.0%
TTGATGCCAGGTATTTGCAA+AGG - chr2.4:48637764-48637783 None:intergenic 40.0%
TTGGTTGAGGTAGTTGTTTG+TGG - chr2.4:48637792-48637811 None:intergenic 40.0%
TTTCCAAAACCACACAAAGG+AGG - chr2.4:48638331-48638350 None:intergenic 40.0%
! ATCTTAGGCCATAGTTCTGT+AGG - chr2.4:48637638-48637657 None:intergenic 40.0%
! CAATTTTCACCTCCTTTGTG+TGG + chr2.4:48638319-48638338 MS.gene64423:CDS 40.0%
! TCTTAGGCCATAGTTCTGTA+GGG - chr2.4:48637637-48637656 None:intergenic 40.0%
!! AGAAGAAGAAGGTGCTAAAC+TGG - chr2.4:48637858-48637877 None:intergenic 40.0%
AAGACACCAAGATCGATACG+CGG + chr2.4:48638441-48638460 MS.gene64423:CDS 45.0%
ACACTACTCCAAGGAGAGTT+AGG - chr2.4:48638106-48638125 None:intergenic 45.0%
ACGAAAGCGAAAACGTGAGA+AGG + chr2.4:48638404-48638423 MS.gene64423:CDS 45.0%
CAATCCTCAACACTACTCCA+AGG - chr2.4:48638115-48638134 None:intergenic 45.0%
CTCAACTCCCTACAGAACTA+TGG + chr2.4:48637627-48637646 MS.gene64423:CDS 45.0%
GAGTTGGAACTTGCATCAGT+TGG - chr2.4:48637836-48637855 None:intergenic 45.0%
GGATGAAGAATGGGGGAAAT+GGG + chr2.4:48638492-48638511 MS.gene64423:CDS 45.0%
TGGATGAAGAATGGGGGAAA+TGG + chr2.4:48638491-48638510 MS.gene64423:CDS 45.0%
TTTGTGGAGATGTTGATGCC+AGG - chr2.4:48637776-48637795 None:intergenic 45.0%
! CTTGCATCGACAACACTTTG+TGG + chr2.4:48638689-48638708 MS.gene64423:CDS 45.0%
! TCACCTCCTTTGTGTGGTTT+TGG + chr2.4:48638325-48638344 MS.gene64423:CDS 45.0%
!! AGAAGGTGCTAAACTGGAGT+TGG - chr2.4:48637852-48637871 None:intergenic 45.0%
!! CACAAAGTGTTGTCGATGCA+AGG - chr2.4:48638691-48638710 None:intergenic 45.0%
!! GAAGTGTTGTTCCTGCTAAG+AGG - chr2.4:48637974-48637993 None:intergenic 45.0%
CTCTCCTTGGAGTAGTGTTG+AGG + chr2.4:48638108-48638127 MS.gene64423:CDS 50.0%
!! CTTTGTGGTGTCATGCACCT+CGG + chr2.4:48638704-48638723 MS.gene64423:CDS 50.0%
CCAAGATCGATACGCGGTTG+TGG + chr2.4:48638447-48638466 MS.gene64423:CDS 55.0%
! CCACAACCGCGTATCGATCT+TGG - chr2.4:48638450-48638469 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 48637548 48638738 48637548 ID=MS.gene64423
chr2.4 mRNA 48637548 48638738 48637548 ID=MS.gene64423.t1;Parent=MS.gene64423
chr2.4 exon 48637548 48638738 48637548 ID=MS.gene64423.t1.exon1;Parent=MS.gene64423.t1
chr2.4 CDS 48637548 48638738 48637548 ID=cds.MS.gene64423.t1;Parent=MS.gene64423.t1
Gene Sequence

>MS.gene64423

ATGATATCACTCTTTAGCAAAGTCAACAAATCTTTGAGATCTGATTCAACTTCAGCCATGGATGAGAAAGTAGATTGGTCTCAACTCCCTACAGAACTATGGCCTAAGATAGGAAAATATCTTGATAATCACATAGATGTTCTCAGATTTCGCAGTGTATGCGAATCATTTCGTTCTTCCATTCCTCATTCTCTTCCAAATTCTCCTTCTTTTCCTTTGCAAATACCTGGCATCAACATCTCCACAAACAACTACCTCAACCAATCAACCATTTATCTTATTCAACCAACTGATGCAAGTTCCAACTCCAGTTTAGCACCTTCTTCTTCTTCTTCTTCCAAAGGTTGGTTGATTAAGGTAGAAGAATCTAAAAATCAACCTTTGAGTTTGTTGAATCCTATCACCGAAAGAAAATTATCATATCCTCTTAGCAGGAACAACACTTCCCTGATGATATGGAACATGTTGGATTATAGGGTCATTGAGTTATGCAAATCATATAAAATTGAAAAGATTAAAGGTTTTTCAAGTCCTGTGAAGAAAGTTGTGTTCTATCCTAACTCTCCTTGGAGTAGTGTTGAGGATTGTGTTTCTTGTTGTATCTTTCAAGAAGATATAATAGGATTCATAAAACATGGTGATGAGAAATGGAAACTTGTCGATGAAAACAATTTCTACTACGATGATATTATTGTGTTCAAGGGCCAGTTTTATGTTACTGATAAATGGGGAACTATTTCATGGATTGATATTTCTTCTTTGAAGTTGATACAATTTTCACCTCCTTTGTGTGGTTTTGGAAACAAGAAACATTTGGTGGAATCTTGTGGGAATCTTTATGTCATTGATAGATACTACGAAAGCGAAAACGTGAGAAGGAACTATGTTGGAAGAAGACACCAAGATCGATACGCGGTTGTGGAGTGTTTCAAAGTCTATAAGTTGGATGAAGAATGGGGGAAATGGGTTGATGTGAAAAATTTGAAAGATAGAGCCTTCATTTTGAGCCCTAGTTGTAACTTCTCCGTTTCGGCTAAAGAATTAATTGGATATCAAGGGAATTGCATCTACTTTAAGGATTCTTTTAATGTTCGTATGTATAATTTGGATGATAGTAGAATCACTACCGTTGATTTTAATCCTTGCATCGACAACACTTTGTGGTGTCATGCACCTCGGCAGAGATGTTAG
Protein sequence

>MS.gene64423.t1

MISLFSKVNKSLRSDSTSAMDEKVDWSQLPTELWPKIGKYLDNHIDVLRFRSVCESFRSSIPHSLPNSPSFPLQIPGINISTNNYLNQSTIYLIQPTDASSNSSLAPSSSSSSKGWLIKVEESKNQPLSLLNPITERKLSYPLSRNNTSLMIWNMLDYRVIELCKSYKIEKIKGFSSPVKKVVFYPNSPWSSVEDCVSCCIFQEDIIGFIKHGDEKWKLVDENNFYYDDIIVFKGQFYVTDKWGTISWIDISSLKLIQFSPPLCGFGNKKHLVESCGNLYVIDRYYESENVRRNYVGRRHQDRYAVVECFKVYKLDEEWGKWVDVKNLKDRAFILSPSCNFSVSAKELIGYQGNCIYFKDSFNVRMYNLDDSRITTVDFNPCIDNTLWCHAPRQRC