Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28904.t1 | XP_003629211.1 | 95 | 378 | 19 | 0 | 1 | 378 | 20 | 397 | 1.20E-220 | 775.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28904.t1 | Q3EBZ2 | 30.4 | 405 | 235 | 13 | 4 | 376 | 1 | 390 | 6.9e-39 | 162.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28904.t1 | G7LA96 | 95.0 | 378 | 19 | 0 | 1 | 378 | 20 | 397 | 8.7e-221 | 775.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049567 | MS.gene28904 | 0.803242 | 3.83E-49 | -1.69E-46 |
| MS.gene049958 | MS.gene28904 | 0.802353 | 5.84E-49 | -1.69E-46 |
| MS.gene050780 | MS.gene28904 | 0.811722 | 6.07E-51 | -1.69E-46 |
| MS.gene051207 | MS.gene28904 | 0.801059 | 1.08E-48 | -1.69E-46 |
| MS.gene051492 | MS.gene28904 | 0.80712 | 5.90E-50 | -1.69E-46 |
| MS.gene052208 | MS.gene28904 | 0.807402 | 5.14E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene28904 | 0.810148 | 1.33E-50 | -1.69E-46 |
| MS.gene053094 | MS.gene28904 | 0.80345 | 3.47E-49 | -1.69E-46 |
| MS.gene053643 | MS.gene28904 | 0.801065 | 1.07E-48 | -1.69E-46 |
| MS.gene053647 | MS.gene28904 | 0.81904 | 1.43E-52 | -1.69E-46 |
| MS.gene05410 | MS.gene28904 | 0.814744 | 1.32E-51 | -1.69E-46 |
| MS.gene05414 | MS.gene28904 | 0.815963 | 7.06E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene28904 | 0.826627 | 2.45E-54 | -1.69E-46 |
| MS.gene05425 | MS.gene28904 | 0.823947 | 1.05E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene28904 | -0.813678 | 2.27E-51 | -1.69E-46 |
| MS.gene05439 | MS.gene28904 | 0.804322 | 2.28E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene28904 | 0.802984 | 4.33E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene28904 | 0.807013 | 6.22E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene44191 | MS.gene28904 | PPI |
| MS.gene44190 | MS.gene28904 | PPI |
| MS.gene049886 | MS.gene28904 | PPI |
| MS.gene23495 | MS.gene28904 | PPI |
| MS.gene97013 | MS.gene28904 | PPI |
| MS.gene28904 | MS.gene017581 | PPI |
| MS.gene28904 | MS.gene023531 | PPI |
| MS.gene012159 | MS.gene28904 | PPI |
| MS.gene28904 | MS.gene018718 | PPI |
| MS.gene64423 | MS.gene28904 | PPI |
| MS.gene037197 | MS.gene28904 | PPI |
| MS.gene30094 | MS.gene28904 | PPI |
| MS.gene91760 | MS.gene28904 | PPI |
| MS.gene28904 | MS.gene012160 | PPI |
| MS.gene28904 | MS.gene017582 | PPI |
| MS.gene28904 | MS.gene39445 | PPI |
| MS.gene28903 | MS.gene28904 | PPI |
| MS.gene28904 | MS.gene064059 | PPI |
| MS.gene28904 | MS.gene08770 | PPI |
| MS.gene28904 | MS.gene025209 | PPI |
| MS.gene23497 | MS.gene28904 | PPI |
| MS.gene28904 | MS.gene66307 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28904.t1 | MTR_8g074610 | 94.974 | 378 | 19 | 0 | 1 | 378 | 20 | 397 | 0.0 | 750 |
| MS.gene28904.t1 | MTR_2g041510 | 76.316 | 380 | 80 | 4 | 1 | 378 | 21 | 392 | 0.0 | 585 |
| MS.gene28904.t1 | MTR_2g041510 | 76.316 | 380 | 80 | 4 | 1 | 378 | 1 | 372 | 0.0 | 584 |
| MS.gene28904.t1 | MTR_8g074640 | 74.142 | 379 | 95 | 3 | 1 | 378 | 1 | 377 | 0.0 | 580 |
| MS.gene28904.t1 | MTR_8g085760 | 33.162 | 389 | 212 | 12 | 2 | 364 | 9 | 375 | 1.41e-51 | 177 |
| MS.gene28904.t1 | MTR_6g092570 | 34.133 | 375 | 200 | 10 | 2 | 364 | 4 | 343 | 6.61e-51 | 174 |
| MS.gene28904.t1 | MTR_5g079830 | 34.746 | 354 | 182 | 10 | 1 | 340 | 1 | 319 | 2.23e-50 | 174 |
| MS.gene28904.t1 | MTR_7g096720 | 34.973 | 366 | 189 | 14 | 1 | 340 | 6 | 348 | 8.96e-48 | 166 |
| MS.gene28904.t1 | MTR_4g088925 | 34.853 | 373 | 187 | 12 | 14 | 364 | 34 | 372 | 1.51e-47 | 166 |
| MS.gene28904.t1 | MTR_3g030050 | 30.969 | 423 | 226 | 14 | 4 | 376 | 14 | 420 | 3.13e-44 | 158 |
| MS.gene28904.t1 | MTR_2g047180 | 28.721 | 383 | 242 | 10 | 5 | 376 | 3 | 365 | 2.40e-33 | 128 |
| MS.gene28904.t1 | MTR_6g018390 | 29.888 | 358 | 193 | 14 | 4 | 340 | 3 | 323 | 1.27e-31 | 123 |
| MS.gene28904.t1 | MTR_1g016570 | 27.320 | 388 | 216 | 11 | 6 | 376 | 21 | 359 | 2.04e-31 | 122 |
| MS.gene28904.t1 | MTR_1g026850 | 29.032 | 403 | 219 | 16 | 1 | 376 | 1 | 363 | 7.51e-30 | 118 |
| MS.gene28904.t1 | MTR_6g027100 | 27.873 | 409 | 223 | 14 | 5 | 377 | 4 | 376 | 8.33e-30 | 119 |
| MS.gene28904.t1 | MTR_1g007260 | 27.034 | 381 | 215 | 12 | 5 | 356 | 21 | 367 | 2.23e-29 | 117 |
| MS.gene28904.t1 | MTR_3g029650 | 28.291 | 357 | 189 | 11 | 5 | 340 | 4 | 314 | 2.42e-29 | 120 |
| MS.gene28904.t1 | MTR_3g029650 | 25.065 | 383 | 238 | 11 | 5 | 357 | 371 | 734 | 2.23e-22 | 99.4 |
| MS.gene28904.t1 | MTR_6g027040 | 28.288 | 403 | 231 | 17 | 5 | 378 | 4 | 377 | 3.23e-29 | 117 |
| MS.gene28904.t1 | MTR_3g058460 | 29.231 | 390 | 228 | 15 | 6 | 376 | 15 | 375 | 3.36e-28 | 114 |
| MS.gene28904.t1 | MTR_3g030360 | 27.273 | 396 | 219 | 15 | 3 | 376 | 2 | 350 | 2.78e-27 | 110 |
| MS.gene28904.t1 | MTR_6g018360 | 27.346 | 373 | 215 | 13 | 5 | 355 | 4 | 342 | 1.67e-26 | 110 |
| MS.gene28904.t1 | MTR_3g030080 | 26.629 | 353 | 210 | 11 | 6 | 342 | 7 | 326 | 2.75e-25 | 105 |
| MS.gene28904.t1 | MTR_1g049170 | 25.352 | 426 | 225 | 15 | 1 | 376 | 1 | 383 | 2.86e-25 | 105 |
| MS.gene28904.t1 | MTR_3g030380 | 26.738 | 374 | 219 | 12 | 5 | 360 | 4 | 340 | 1.29e-24 | 103 |
| MS.gene28904.t1 | MTR_4g125090 | 27.381 | 336 | 165 | 11 | 2 | 321 | 41 | 313 | 4.76e-24 | 101 |
| MS.gene28904.t1 | MTR_3g029620 | 26.842 | 380 | 225 | 13 | 4 | 362 | 1 | 348 | 5.21e-24 | 102 |
| MS.gene28904.t1 | MTR_3g114990 | 28.291 | 357 | 219 | 12 | 5 | 344 | 22 | 358 | 1.43e-23 | 101 |
| MS.gene28904.t1 | MTR_3g029640 | 26.772 | 381 | 212 | 15 | 5 | 362 | 3 | 339 | 2.46e-23 | 100 |
| MS.gene28904.t1 | MTR_3g037710 | 31.034 | 261 | 130 | 8 | 121 | 357 | 9 | 243 | 3.05e-23 | 98.2 |
| MS.gene28904.t1 | MTR_3g030480 | 26.330 | 376 | 216 | 13 | 5 | 360 | 4 | 338 | 3.71e-23 | 99.8 |
| MS.gene28904.t1 | MTR_3g091990 | 27.072 | 362 | 211 | 13 | 5 | 340 | 6 | 340 | 1.71e-22 | 98.6 |
| MS.gene28904.t1 | MTR_3g030355 | 26.812 | 414 | 219 | 18 | 1 | 363 | 1 | 381 | 2.67e-22 | 97.8 |
| MS.gene28904.t1 | MTR_6g018400 | 26.257 | 358 | 203 | 10 | 3 | 338 | 2 | 320 | 3.26e-22 | 97.1 |
| MS.gene28904.t1 | MTR_3g029840 | 26.593 | 361 | 195 | 15 | 6 | 342 | 5 | 319 | 4.11e-22 | 96.7 |
| MS.gene28904.t1 | MTR_3g030440 | 25.789 | 380 | 221 | 13 | 1 | 360 | 1 | 339 | 1.10e-21 | 95.5 |
| MS.gene28904.t1 | MTR_3g029670 | 27.612 | 402 | 216 | 16 | 5 | 363 | 4 | 373 | 1.42e-21 | 95.5 |
| MS.gene28904.t1 | MTR_3g029920 | 28.392 | 398 | 217 | 15 | 5 | 375 | 8 | 364 | 1.60e-21 | 95.5 |
| MS.gene28904.t1 | MTR_6g018315 | 25.888 | 394 | 229 | 14 | 18 | 377 | 2 | 366 | 4.51e-21 | 94.4 |
| MS.gene28904.t1 | MTR_3g029950 | 27.467 | 375 | 185 | 15 | 5 | 346 | 4 | 324 | 1.13e-20 | 92.8 |
| MS.gene28904.t1 | MTR_3g092000 | 25.679 | 405 | 216 | 16 | 5 | 362 | 6 | 372 | 2.82e-20 | 92.0 |
| MS.gene28904.t1 | MTR_3g030560 | 26.238 | 404 | 232 | 17 | 5 | 376 | 3 | 372 | 6.58e-20 | 90.5 |
| MS.gene28904.t1 | MTR_3g114970 | 25.581 | 387 | 210 | 16 | 5 | 363 | 3 | 339 | 1.17e-19 | 89.7 |
| MS.gene28904.t1 | MTR_3g029750 | 25.326 | 383 | 216 | 12 | 5 | 351 | 4 | 352 | 7.78e-19 | 87.4 |
| MS.gene28904.t1 | MTR_3g047980 | 28.191 | 376 | 219 | 14 | 6 | 376 | 30 | 359 | 9.22e-18 | 84.3 |
| MS.gene28904.t1 | MTR_3g029900 | 31.214 | 173 | 83 | 5 | 199 | 368 | 297 | 436 | 5.47e-15 | 76.6 |
| MS.gene28904.t1 | MTR_1g016540 | 29.630 | 189 | 103 | 6 | 206 | 376 | 2 | 178 | 6.43e-13 | 67.0 |
| MS.gene28904.t1 | MTR_5g042230 | 33.553 | 152 | 87 | 5 | 191 | 340 | 13 | 152 | 7.14e-12 | 64.7 |
| MS.gene28904.t1 | MTR_6g018370 | 34.884 | 129 | 74 | 4 | 191 | 318 | 132 | 251 | 1.80e-11 | 64.3 |
| MS.gene28904.t1 | MTR_1g007250 | 32.639 | 144 | 80 | 4 | 208 | 348 | 4 | 133 | 3.16e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28904.t1 | AT2G17030 | 30.370 | 405 | 235 | 13 | 4 | 376 | 1 | 390 | 1.90e-43 | 155 |
| MS.gene28904.t1 | AT2G17036 | 28.431 | 408 | 233 | 15 | 4 | 376 | 1 | 384 | 1.82e-37 | 139 |
| MS.gene28904.t1 | AT2G17036 | 28.079 | 406 | 235 | 14 | 5 | 376 | 1 | 383 | 3.14e-37 | 139 |
| MS.gene28904.t1 | AT1G65770 | 28.954 | 373 | 203 | 12 | 4 | 353 | 1 | 334 | 4.59e-35 | 132 |
| MS.gene28904.t1 | AT4G35733 | 28.608 | 388 | 216 | 15 | 1 | 365 | 1 | 350 | 4.57e-33 | 127 |
| MS.gene28904.t1 | AT1G44080 | 27.717 | 368 | 213 | 12 | 5 | 362 | 4 | 328 | 8.26e-30 | 117 |
| MS.gene28904.t1 | AT2G26160 | 29.745 | 353 | 195 | 14 | 6 | 342 | 5 | 320 | 5.44e-29 | 115 |
| MS.gene28904.t1 | AT3G25750 | 26.146 | 371 | 227 | 11 | 3 | 357 | 2 | 341 | 1.74e-26 | 108 |
| MS.gene28904.t1 | AT3G25750 | 27.838 | 370 | 208 | 13 | 3 | 357 | 2 | 327 | 2.86e-25 | 105 |
| MS.gene28904.t1 | AT2G17690 | 28.436 | 422 | 217 | 15 | 6 | 360 | 3 | 406 | 1.86e-24 | 104 |
| MS.gene28904.t1 | AT1G65740 | 27.926 | 376 | 215 | 14 | 4 | 353 | 1 | 346 | 6.05e-24 | 102 |
| MS.gene28904.t1 | AT1G65760 | 28.142 | 366 | 211 | 14 | 6 | 353 | 5 | 336 | 2.75e-23 | 100 |
| MS.gene28904.t1 | AT2G16290 | 26.485 | 404 | 234 | 17 | 4 | 364 | 1 | 384 | 4.52e-20 | 91.7 |
| MS.gene28904.t1 | AT5G24040 | 23.450 | 371 | 211 | 13 | 4 | 344 | 1 | 328 | 6.98e-15 | 75.9 |
| MS.gene28904.t1 | AT1G64840 | 24.650 | 357 | 216 | 13 | 6 | 342 | 4 | 327 | 2.94e-13 | 70.9 |
Find 72 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGTTCGTATGTATAATT+TGG | 0.212475 | 8.2:+30154215 | MS.gene28904:CDS |
| AATGGAAAAGAAGGAGAATT+TGG | 0.229744 | 8.2:-30153322 | None:intergenic |
| TCACCTCCTTTGTGTGGATT+TGG | 0.230521 | 8.2:+30153907 | MS.gene28904:CDS |
| AAGGGAACTGCATCTACTTT+AGG | 0.247416 | 8.2:+30154184 | MS.gene28904:CDS |
| ATCTTTCAAGAGGGTAAATT+AGG | 0.264749 | 8.2:+30153730 | MS.gene28904:CDS |
| GTCTCAACTAAAGACTTAAT+TGG | 0.265830 | 8.2:+30154156 | MS.gene28904:CDS |
| TGGATGAAGAATGGGGAAAA+TGG | 0.270862 | 8.2:+30154073 | MS.gene28904:CDS |
| TTCCAAAGGTTGGCTGATTA+AGG | 0.291497 | 8.2:+30153465 | MS.gene28904:CDS |
| AGGTGCTAAAGGTTCTAAAT+TGG | 0.350268 | 8.2:-30153437 | None:intergenic |
| ATAGGACTCAACAAACTTAC+AGG | 0.370665 | 8.2:-30153508 | None:intergenic |
| ATAAATGGGGAACTATTTCA+TGG | 0.373052 | 8.2:+30153851 | MS.gene28904:CDS |
| AGGGAACTGCATCTACTTTA+GGG | 0.379511 | 8.2:+30154185 | MS.gene28904:CDS |
| TATGGAACTTGATGGATCAT+AGG | 0.381154 | 8.2:+30153584 | MS.gene28904:CDS |
| CTTGCATTGACAAGACTTTG+TGG | 0.381970 | 8.2:+30154271 | MS.gene28904:CDS |
| CAATGATATAAACGGTTGAT+TGG | 0.387856 | 8.2:-30153390 | None:intergenic |
| ACACTAGTCCAAGGAGAGTT+AGG | 0.390020 | 8.2:-30153685 | None:intergenic |
| TTATAAGTTGGATGAAGAAT+GGG | 0.394790 | 8.2:+30154065 | MS.gene28904:CDS |
| TACCTTAATCAGCCAACCTT+TGG | 0.397147 | 8.2:-30153467 | None:intergenic |
| TTTATAAGTTGGATGAAGAA+TGG | 0.397683 | 8.2:+30154064 | MS.gene28904:CDS |
| CACCTTCTTCTTCCAAAGGT+TGG | 0.399560 | 8.2:+30153455 | MS.gene28904:CDS |
| AAAGACTTAATTGGATATCA+AGG | 0.406036 | 8.2:+30154165 | MS.gene28904:CDS |
| GGATGATCATAGAATCACTA+TGG | 0.406868 | 8.2:+30154236 | MS.gene28904:CDS |
| GAAGGAGAATTTGGATGAGA+TGG | 0.414677 | 8.2:-30153313 | None:intergenic |
| TGGAAACAAGAAGCATTTGA+TGG | 0.415140 | 8.2:+30153927 | MS.gene28904:CDS |
| ATCTTAGGCCATAGCTCTAT+AGG | 0.421126 | 8.2:-30153214 | None:intergenic |
| TACCATGCTATGGAACTTGA+TGG | 0.430964 | 8.2:+30153576 | MS.gene28904:CDS |
| AGTTGGTTCAATGATATAAA+CGG | 0.439030 | 8.2:-30153398 | None:intergenic |
| TGACGATGTTATTGTGTTCA+AGG | 0.452162 | 8.2:+30153810 | MS.gene28904:CDS |
| ATAGAGCTATGGCCTAAGAT+AGG | 0.461985 | 8.2:+30153217 | MS.gene28904:CDS |
| ATGTTTCAAAGTTTATAAGT+TGG | 0.473392 | 8.2:+30154053 | MS.gene28904:CDS |
| GGATGAAGAATGGGGAAAAT+GGG | 0.473828 | 8.2:+30154074 | MS.gene28904:CDS |
| CTTTGTGGTCTCGTTCACCT+TGG | 0.488154 | 8.2:+30154286 | MS.gene28904:CDS |
| TGTTCTATCCTAACTCTCCT+TGG | 0.489941 | 8.2:+30153677 | MS.gene28904:CDS |
| TTGTTTAGCGAGTCATAGAC+GGG | 0.490768 | 8.2:-30153358 | None:intergenic |
| GGAGAATTTGGATGAGATGG+AGG | 0.496344 | 8.2:-30153310 | None:intergenic |
| TTGGAAGAAGAAGGTGCTAA+AGG | 0.501406 | 8.2:-30153448 | None:intergenic |
| ACGGGGTGAGGTATTTGTAA+TGG | 0.513372 | 8.2:-30153340 | None:intergenic |
| GGTATTTGTAATGGAAAAGA+AGG | 0.516150 | 8.2:-30153331 | None:intergenic |
| ATTTGGATGAGATGGAGGAA+TGG | 0.516975 | 8.2:-30153305 | None:intergenic |
| TAAAGGTTCTAAATTGGAGT+TGG | 0.521984 | 8.2:-30153431 | None:intergenic |
| GAAGTGTTGTTACTGCTAAG+AGG | 0.538395 | 8.2:-30153553 | None:intergenic |
| CAATTCTCACCTCCTTTGTG+TGG | 0.538436 | 8.2:+30153901 | MS.gene28904:CDS |
| CTATGAAAGTAGTGAAGACA+TGG | 0.542033 | 8.2:+30153984 | MS.gene28904:CDS |
| CTCAACTTCCTATAGAGCTA+TGG | 0.544377 | 8.2:+30153206 | MS.gene28904:CDS |
| ATGGAACTTGATGGATCATA+GGG | 0.547361 | 8.2:+30153585 | MS.gene28904:CDS |
| CCAATCAACTATCTCATCCA+TGG | 0.548037 | 8.2:-30153182 | None:intergenic |
| CCATGGATGAGATAGTTGAT+TGG | 0.548664 | 8.2:+30153182 | None:intergenic |
| GTTGTTTAGCGAGTCATAGA+CGG | 0.549312 | 8.2:-30153359 | None:intergenic |
| ACAACACTTCTACCATGCTA+TGG | 0.549319 | 8.2:+30153566 | MS.gene28904:CDS |
| TTAGCACCTTCTTCTTCCAA+AGG | 0.559083 | 8.2:+30153451 | MS.gene28904:CDS |
| ATCCATCAAGTTCCATAGCA+TGG | 0.559240 | 8.2:-30153578 | None:intergenic |
| AGCCAACCTTTGGAAGAAGA+AGG | 0.565959 | 8.2:-30153457 | None:intergenic |
| GACGATGTTATTGTGTTCAA+GGG | 0.569592 | 8.2:+30153811 | MS.gene28904:CDS |
| CACAAAGTCTTGTCAATGCA+AGG | 0.569640 | 8.2:-30154270 | None:intergenic |
| AAGTGTTGTTACTGCTAAGA+GGG | 0.570047 | 8.2:-30153552 | None:intergenic |
| AGCGAGTCATAGACGGGGTG+AGG | 0.574176 | 8.2:-30153352 | None:intergenic |
| TATAAGTTGGATGAAGAATG+GGG | 0.581944 | 8.2:+30154066 | MS.gene28904:CDS |
| CTCTCCTTGGACTAGTGTTG+AGG | 0.583954 | 8.2:+30153690 | MS.gene28904:CDS |
| GAGTTGGAACTTGCATCAGT+TGG | 0.584443 | 8.2:-30153415 | None:intergenic |
| AGCTTGTTGTATCTTTCAAG+AGG | 0.595503 | 8.2:+30153720 | MS.gene28904:CDS |
| GTCATCTAACATCTCATCCA+AGG | 0.596455 | 8.2:-30154303 | None:intergenic |
| AAAGTAGTGAAGACATGGGA+AGG | 0.596556 | 8.2:+30153989 | MS.gene28904:CDS |
| AAGACTTAATTGGATATCAA+GGG | 0.598333 | 8.2:+30154166 | MS.gene28904:CDS |
| GCTTGTTGTATCTTTCAAGA+GGG | 0.601791 | 8.2:+30153721 | MS.gene28904:CDS |
| TTTCCAAATCCACACAAAGG+AGG | 0.638096 | 8.2:-30153910 | None:intergenic |
| CTACGATCGAGACACTGATG+TGG | 0.646731 | 8.2:+30154029 | MS.gene28904:CDS |
| AAATTAGGAATCATGAAACA+TGG | 0.647692 | 8.2:+30153745 | MS.gene28904:CDS |
| CATTCCTCAACACTAGTCCA+AGG | 0.647862 | 8.2:-30153694 | None:intergenic |
| TTGTTTCCAAATCCACACAA+AGG | 0.650065 | 8.2:-30153913 | None:intergenic |
| AAGCATTTGATGGAGTCATG+TGG | 0.661502 | 8.2:+30153937 | MS.gene28904:CDS |
| TATGAAAGTAGTGAAGACAT+GGG | 0.714849 | 8.2:+30153985 | MS.gene28904:CDS |
| TGTTTAGCGAGTCATAGACG+GGG | 0.771740 | 8.2:-30153357 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTTCAAAGTTTATAAGT+TGG | + | chr8.2:30154053-30154072 | MS.gene28904:CDS | 20.0% |
| !!! | AGTTTTATGTTACTGATAAA+TGG | + | chr8.2:30153836-30153855 | MS.gene28904:CDS | 20.0% |
| !!! | GTTTTATGTTACTGATAAAT+GGG | + | chr8.2:30153837-30153856 | MS.gene28904:CDS | 20.0% |
| !!! | TTTTATGTTACTGATAAATG+GGG | + | chr8.2:30153838-30153857 | MS.gene28904:CDS | 20.0% |
| ! | AAAGACTTAATTGGATATCA+AGG | + | chr8.2:30154165-30154184 | MS.gene28904:CDS | 25.0% |
| ! | AAATTAGGAATCATGAAACA+TGG | + | chr8.2:30153745-30153764 | MS.gene28904:CDS | 25.0% |
| ! | AAGACTTAATTGGATATCAA+GGG | + | chr8.2:30154166-30154185 | MS.gene28904:CDS | 25.0% |
| ! | TGATGTTCGTATGTATAATT+TGG | + | chr8.2:30154215-30154234 | MS.gene28904:CDS | 25.0% |
| !! | AGTTGGTTCAATGATATAAA+CGG | - | chr8.2:30153401-30153420 | None:intergenic | 25.0% |
| !! | TCAAGATATTTTCCTATCTT+AGG | - | chr8.2:30153232-30153251 | None:intergenic | 25.0% |
| !! | TTATAAGTTGGATGAAGAAT+GGG | + | chr8.2:30154065-30154084 | MS.gene28904:CDS | 25.0% |
| !! | TTTATAAGTTGGATGAAGAA+TGG | + | chr8.2:30154064-30154083 | MS.gene28904:CDS | 25.0% |
| !!! | TAAGAGGGTATGATAATTTT+CGG | - | chr8.2:30153540-30153559 | None:intergenic | 25.0% |
| AATGGAAAAGAAGGAGAATT+TGG | - | chr8.2:30153325-30153344 | None:intergenic | 30.0% | |
| ATAAATGGGGAACTATTTCA+TGG | + | chr8.2:30153851-30153870 | MS.gene28904:CDS | 30.0% | |
| ATCTTTCAAGAGGGTAAATT+AGG | + | chr8.2:30153730-30153749 | MS.gene28904:CDS | 30.0% | |
| CAATGATATAAACGGTTGAT+TGG | - | chr8.2:30153393-30153412 | None:intergenic | 30.0% | |
| GTCTCAACTAAAGACTTAAT+TGG | + | chr8.2:30154156-30154175 | MS.gene28904:CDS | 30.0% | |
| TATGAAAGTAGTGAAGACAT+GGG | + | chr8.2:30153985-30154004 | MS.gene28904:CDS | 30.0% | |
| ! | GGTATTTGTAATGGAAAAGA+AGG | - | chr8.2:30153334-30153353 | None:intergenic | 30.0% |
| ! | TATAAGTTGGATGAAGAATG+GGG | + | chr8.2:30154066-30154085 | MS.gene28904:CDS | 30.0% |
| ! | TGAAACATGGTGATGAAAAA+TGG | + | chr8.2:30153758-30153777 | MS.gene28904:CDS | 30.0% |
| !! | TAAAGGTTCTAAATTGGAGT+TGG | - | chr8.2:30153434-30153453 | None:intergenic | 30.0% |
| ATAGGACTCAACAAACTTAC+AGG | - | chr8.2:30153511-30153530 | None:intergenic | 35.0% | |
| CTATGAAAGTAGTGAAGACA+TGG | + | chr8.2:30153984-30154003 | MS.gene28904:CDS | 35.0% | |
| GACGATGTTATTGTGTTCAA+GGG | + | chr8.2:30153811-30153830 | MS.gene28904:CDS | 35.0% | |
| GGATGATCATAGAATCACTA+TGG | + | chr8.2:30154236-30154255 | MS.gene28904:CDS | 35.0% | |
| TGACGATGTTATTGTGTTCA+AGG | + | chr8.2:30153810-30153829 | MS.gene28904:CDS | 35.0% | |
| TGATGAAAAATGGACACTTG+TGG | + | chr8.2:30153768-30153787 | MS.gene28904:CDS | 35.0% | |
| TTGTTTCCAAATCCACACAA+AGG | - | chr8.2:30153916-30153935 | None:intergenic | 35.0% | |
| ! | AAGTGTTGTTACTGCTAAGA+GGG | - | chr8.2:30153555-30153574 | None:intergenic | 35.0% |
| ! | ATGGAACTTGATGGATCATA+GGG | + | chr8.2:30153585-30153604 | MS.gene28904:CDS | 35.0% |
| ! | GATAATTTTCGGTCAGAGAT+AGG | - | chr8.2:30153529-30153548 | None:intergenic | 35.0% |
| ! | TATGGAACTTGATGGATCAT+AGG | + | chr8.2:30153584-30153603 | MS.gene28904:CDS | 35.0% |
| !! | AGCTTGTTGTATCTTTCAAG+AGG | + | chr8.2:30153720-30153739 | MS.gene28904:CDS | 35.0% |
| !! | AGGTGCTAAAGGTTCTAAAT+TGG | - | chr8.2:30153440-30153459 | None:intergenic | 35.0% |
| !! | GCTTGTTGTATCTTTCAAGA+GGG | + | chr8.2:30153721-30153740 | MS.gene28904:CDS | 35.0% |
| !! | TGGAAACAAGAAGCATTTGA+TGG | + | chr8.2:30153927-30153946 | MS.gene28904:CDS | 35.0% |
| AAAGTAGTGAAGACATGGGA+AGG | + | chr8.2:30153989-30154008 | MS.gene28904:CDS | 40.0% | |
| AAGCATTTGATGGAGTCATG+TGG | + | chr8.2:30153937-30153956 | MS.gene28904:CDS | 40.0% | |
| AAGGGAACTGCATCTACTTT+AGG | + | chr8.2:30154184-30154203 | MS.gene28904:CDS | 40.0% | |
| ACAACACTTCTACCATGCTA+TGG | + | chr8.2:30153566-30153585 | MS.gene28904:CDS | 40.0% | |
| AGGGAACTGCATCTACTTTA+GGG | + | chr8.2:30154185-30154204 | MS.gene28904:CDS | 40.0% | |
| ATAGAGCTATGGCCTAAGAT+AGG | + | chr8.2:30153217-30153236 | MS.gene28904:CDS | 40.0% | |
| ATCCATCAAGTTCCATAGCA+TGG | - | chr8.2:30153581-30153600 | None:intergenic | 40.0% | |
| ATTTGGATGAGATGGAGGAA+TGG | - | chr8.2:30153308-30153327 | None:intergenic | 40.0% | |
| CTCAACTTCCTATAGAGCTA+TGG | + | chr8.2:30153206-30153225 | MS.gene28904:CDS | 40.0% | |
| GAAGGAGAATTTGGATGAGA+TGG | - | chr8.2:30153316-30153335 | None:intergenic | 40.0% | |
| GGATGAAGAATGGGGAAAAT+GGG | + | chr8.2:30154074-30154093 | MS.gene28904:CDS | 40.0% | |
| GTTGTTTAGCGAGTCATAGA+CGG | - | chr8.2:30153362-30153381 | None:intergenic | 40.0% | |
| TACCATGCTATGGAACTTGA+TGG | + | chr8.2:30153576-30153595 | MS.gene28904:CDS | 40.0% | |
| TACCTTAATCAGCCAACCTT+TGG | - | chr8.2:30153470-30153489 | None:intergenic | 40.0% | |
| TGGATGAAGAATGGGGAAAA+TGG | + | chr8.2:30154073-30154092 | MS.gene28904:CDS | 40.0% | |
| TGTTCTATCCTAACTCTCCT+TGG | + | chr8.2:30153677-30153696 | MS.gene28904:CDS | 40.0% | |
| TTAGCACCTTCTTCTTCCAA+AGG | + | chr8.2:30153451-30153470 | MS.gene28904:CDS | 40.0% | |
| TTGTTTAGCGAGTCATAGAC+GGG | - | chr8.2:30153361-30153380 | None:intergenic | 40.0% | |
| TTTCCAAATCCACACAAAGG+AGG | - | chr8.2:30153913-30153932 | None:intergenic | 40.0% | |
| ! | ATCTTAGGCCATAGCTCTAT+AGG | - | chr8.2:30153217-30153236 | None:intergenic | 40.0% |
| ! | CACAAAGTCTTGTCAATGCA+AGG | - | chr8.2:30154273-30154292 | None:intergenic | 40.0% |
| ! | CTTGCATTGACAAGACTTTG+TGG | + | chr8.2:30154271-30154290 | MS.gene28904:CDS | 40.0% |
| ! | TTCCAAAGGTTGGCTGATTA+AGG | + | chr8.2:30153465-30153484 | MS.gene28904:CDS | 40.0% |
| !! | GAAGTGTTGTTACTGCTAAG+AGG | - | chr8.2:30153556-30153575 | None:intergenic | 40.0% |
| !! | TTGGAAGAAGAAGGTGCTAA+AGG | - | chr8.2:30153451-30153470 | None:intergenic | 40.0% |
| ACACTAGTCCAAGGAGAGTT+AGG | - | chr8.2:30153688-30153707 | None:intergenic | 45.0% | |
| CAATTCTCACCTCCTTTGTG+TGG | + | chr8.2:30153901-30153920 | MS.gene28904:CDS | 45.0% | |
| CACCTTCTTCTTCCAAAGGT+TGG | + | chr8.2:30153455-30153474 | MS.gene28904:CDS | 45.0% | |
| CATTCCTCAACACTAGTCCA+AGG | - | chr8.2:30153697-30153716 | None:intergenic | 45.0% | |
| GAGTTGGAACTTGCATCAGT+TGG | - | chr8.2:30153418-30153437 | None:intergenic | 45.0% | |
| GGAGAATTTGGATGAGATGG+AGG | - | chr8.2:30153313-30153332 | None:intergenic | 45.0% | |
| TCACCTCCTTTGTGTGGATT+TGG | + | chr8.2:30153907-30153926 | MS.gene28904:CDS | 45.0% | |
| TGTTTAGCGAGTCATAGACG+GGG | - | chr8.2:30153360-30153379 | None:intergenic | 45.0% | |
| ! | ACGGGGTGAGGTATTTGTAA+TGG | - | chr8.2:30153343-30153362 | None:intergenic | 45.0% |
| ! | AGCCAACCTTTGGAAGAAGA+AGG | - | chr8.2:30153460-30153479 | None:intergenic | 45.0% |
| CTACGATCGAGACACTGATG+TGG | + | chr8.2:30154029-30154048 | MS.gene28904:CDS | 50.0% | |
| CTTTGTGGTCTCGTTCACCT+TGG | + | chr8.2:30154286-30154305 | MS.gene28904:CDS | 50.0% | |
| !! | CTCTCCTTGGACTAGTGTTG+AGG | + | chr8.2:30153690-30153709 | MS.gene28904:CDS | 50.0% |
| AGCGAGTCATAGACGGGGTG+AGG | - | chr8.2:30153355-30153374 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 30153184 | 30154320 | 30153184 | ID=MS.gene28904 |
| chr8.2 | mRNA | 30153184 | 30154320 | 30153184 | ID=MS.gene28904.t1;Parent=MS.gene28904 |
| chr8.2 | exon | 30153184 | 30154320 | 30153184 | ID=MS.gene28904.t1.exon1;Parent=MS.gene28904.t1 |
| chr8.2 | CDS | 30153184 | 30154320 | 30153184 | ID=cds.MS.gene28904.t1;Parent=MS.gene28904.t1 |
| Gene Sequence |
| Protein sequence |
