AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene018718


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018718.t1 MTR_7g096720 97.361 379 10 0 1 379 4 382 0.0 768
MS.gene018718.t1 MTR_4g088925 66.022 362 109 3 18 379 34 381 4.09e-156 444
MS.gene018718.t1 MTR_8g085760 52.196 387 161 9 4 378 9 383 4.43e-125 365
MS.gene018718.t1 MTR_6g092570 43.548 372 167 15 13 375 11 348 3.21e-69 222
MS.gene018718.t1 MTR_5g079830 41.193 352 177 10 13 360 9 334 5.08e-63 207
MS.gene018718.t1 MTR_8g074610 36.554 383 182 17 3 356 20 370 4.77e-50 173
MS.gene018718.t1 MTR_2g041510 35.772 369 182 12 12 356 8 345 3.60e-46 162
MS.gene018718.t1 MTR_2g041510 35.772 369 182 12 12 356 28 365 4.36e-46 162
MS.gene018718.t1 MTR_8g074640 32.440 373 194 11 12 356 8 350 1.41e-45 160
MS.gene018718.t1 MTR_4g125090 38.339 313 139 12 13 322 48 309 2.97e-44 155
MS.gene018718.t1 MTR_7g096760 69.474 95 27 2 225 318 1 94 2.09e-35 127
MS.gene018718.t1 MTR_3g029650 25.858 379 215 11 12 375 7 334 2.98e-17 84.0
MS.gene018718.t1 MTR_3g029650 21.429 378 234 12 4 349 366 712 2.85e-12 68.6
MS.gene018718.t1 MTR_3g029800 23.513 353 208 12 12 345 6 315 1.71e-14 74.3
MS.gene018718.t1 MTR_6g018400 23.056 360 203 13 12 343 7 320 3.74e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018718.t1 AT2G26160 29.714 350 216 11 12 357 7 330 4.24e-34 129
MS.gene018718.t1 AT1G65770 28.571 371 196 11 12 356 5 332 6.26e-34 129
MS.gene018718.t1 AT1G44080 29.830 352 199 9 12 356 7 317 1.53e-31 122
MS.gene018718.t1 AT4G35733 27.606 355 218 8 12 356 8 333 3.29e-28 114
MS.gene018718.t1 AT3G25750 27.095 358 202 12 12 357 7 317 1.73e-23 100
MS.gene018718.t1 AT3G25750 26.722 363 211 13 12 357 7 331 8.92e-23 98.6
MS.gene018718.t1 AT2G17030 24.590 366 216 11 12 345 5 342 4.36e-21 94.4
MS.gene018718.t1 AT1G65740 24.731 372 221 11 12 356 5 344 1.30e-20 92.4
MS.gene018718.t1 AT1G65760 26.146 371 195 12 6 345 1 323 2.10e-20 92.0
MS.gene018718.t1 AT2G17036 24.047 341 221 12 12 334 4 324 1.43e-14 75.1
MS.gene018718.t1 AT2G17036 24.047 341 221 12 12 334 5 325 1.43e-14 75.1
MS.gene018718.t1 AT5G60060 26.136 352 207 17 1 330 1 321 4.95e-12 67.0

Find 62 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACCTGCTGACAAATTT+TGG 0.080030 7.1:+11037866 MS.gene018718:CDS
ACTTTATGTTGTGGATAAAA+TGG 0.170692 7.1:+11037438 MS.gene018718:CDS
GAGTTGACTGTTTCCAATAT+TGG 0.239268 7.1:+11037337 MS.gene018718:CDS
GTGCTGAAAATAGGCATATA+AGG 0.260640 7.1:+11037530 MS.gene018718:CDS
TCCGTAGTGTTTGTAGATTT+TGG 0.264838 7.1:+11036888 MS.gene018718:CDS
AGTCATCATGAAGATAAAAT+TGG 0.275568 7.1:+11037205 MS.gene018718:CDS
TGTACCATTGTGCTGAAAAT+AGG 0.283196 7.1:+11037521 MS.gene018718:CDS
GAGATCAAAGAGATAAAGTT+TGG 0.285701 7.1:-11037086 None:intergenic
TGGTTCAATGCGGTATATTT+TGG 0.305635 7.1:-11036984 None:intergenic
GGGTGAGGTTTCGTTTGTTT+TGG 0.311852 7.1:+11037729 MS.gene018718:CDS
AAAGGAAAATATCATGTTAA+AGG 0.318603 7.1:+11037363 MS.gene018718:CDS
TTGATCAAGAATGGGGTAAA+TGG 0.326226 7.1:+11037689 MS.gene018718:CDS
ATGGTTAGAAGTAAAAGATT+TGG 0.332561 7.1:+11037708 MS.gene018718:CDS
CAAATCTTCTTGAAGATTTG+TGG 0.347804 7.1:+11036819 MS.gene018718:CDS
TGGTTAGAAGTAAAAGATTT+GGG 0.374795 7.1:+11037709 MS.gene018718:CDS
CAATGCTCTTTCCTTGAAGT+TGG 0.381115 7.1:+11037477 MS.gene018718:CDS
CAAAAGGGGTTACTTCTTGA+AGG 0.384442 7.1:+11037642 MS.gene018718:CDS
TTGTGAGAGGCAGGTGGAGG+AGG 0.384893 7.1:-11036923 None:intergenic
AAATCTTCTTGAAGATTTGT+GGG 0.389413 7.1:+11036820 MS.gene018718:CDS
TTTATAAGCTTGATCAAGAA+TGG 0.392244 7.1:+11037680 MS.gene018718:CDS
CTTTATGTTGTGGATAAAAT+GGG 0.396836 7.1:+11037439 MS.gene018718:CDS
TCAACTTCTTGTTCTAATCA+AGG 0.402165 7.1:+11037028 MS.gene018718:CDS
ATGATGAAAGGTATTACAAA+AGG 0.404208 7.1:+11037626 MS.gene018718:CDS
CCAATCCAAAATTTGTCAGC+AGG 0.420447 7.1:-11037871 None:intergenic
TGATGAAAGGTATTACAAAA+GGG 0.432135 7.1:+11037627 MS.gene018718:CDS
TGAAAATTGAACCAACTTCA+AGG 0.432491 7.1:-11037488 None:intergenic
TTGTTGGCTCGAGATTACTA+TGG 0.437431 7.1:+11037772 MS.gene018718:CDS
TGTTTCCAATATTGGAGAAA+AGG 0.445230 7.1:+11037345 MS.gene018718:CDS
TTATAAGCTTGATCAAGAAT+GGG 0.450966 7.1:+11037681 MS.gene018718:CDS
TGATGATATAATACATTACA+AGG 0.467606 7.1:+11037411 MS.gene018718:CDS
TCTACTTCTATTATGAAAGC+AGG 0.486961 7.1:+11037812 MS.gene018718:CDS
TGCTGAAAATAGGCATATAA+GGG 0.494278 7.1:+11037531 MS.gene018718:CDS
CTTACAAATTAATTGATCAC+TGG 0.508472 7.1:-11037910 None:intergenic
ATCACTGGATGGAACAAAGT+TGG 0.510777 7.1:-11037895 None:intergenic
TGATTAGAACAAGAAGTTGA+AGG 0.514698 7.1:-11037025 None:intergenic
ATCAAAGAGATAAAGTTTGG+AGG 0.516104 7.1:-11037083 None:intergenic
GATTAGAACAAGAAGTTGAA+GGG 0.519595 7.1:-11037024 None:intergenic
ATTTATATATTAATGATGAA+AGG 0.523166 7.1:+11037614 MS.gene018718:CDS
CAAGGGACAACTTTATGTTG+TGG 0.530035 7.1:+11037429 MS.gene018718:CDS
ATGCAAAGGTTGTGAGAGGC+AGG 0.537372 7.1:-11036932 None:intergenic
AAGCAATTAGTAGAGTATGA+TGG 0.538440 7.1:+11037574 MS.gene018718:CDS
CAAATTAATTGATCACTGGA+TGG 0.540924 7.1:-11037906 None:intergenic
TGTTGGCTCGAGATTACTAT+GGG 0.552161 7.1:+11037773 MS.gene018718:CDS
AGGTTGTGAGAGGCAGGTGG+AGG 0.559115 7.1:-11036926 None:intergenic
CTTCTTGTTCTAATCAAGGT+GGG 0.559628 7.1:+11037032 MS.gene018718:CDS
GATGAAAGGTATTACAAAAG+GGG 0.559891 7.1:+11037628 MS.gene018718:CDS
AGAAGTAAAAGATTTGGGTG+AGG 0.564111 7.1:+11037714 MS.gene018718:CDS
GGGTTGTGATCATGTGGTGA+TGG 0.571859 7.1:-11037004 None:intergenic
GATGGATATGAATAAGATAG+TGG 0.582410 7.1:+11036784 None:intergenic
GCCAAAATCTACAAACACTA+CGG 0.595515 7.1:-11036889 None:intergenic
CTACTTCTATTATGAAAGCA+GGG 0.600724 7.1:+11037813 MS.gene018718:CDS
ACTTCTTGTTCTAATCAAGG+TGG 0.607280 7.1:+11037031 MS.gene018718:CDS
ATCATGTTAAAGGATGATTG+TGG 0.624512 7.1:+11037373 MS.gene018718:CDS
TATAAGCTTGATCAAGAATG+GGG 0.627945 7.1:+11037682 MS.gene018718:CDS
CAAAGGTTGTGAGAGGCAGG+TGG 0.633368 7.1:-11036929 None:intergenic
GATGATATAATACATTACAA+GGG 0.643392 7.1:+11037412 MS.gene018718:CDS
TAATACGAGAATCCGAGCCG+AGG 0.671509 7.1:+11037114 MS.gene018718:CDS
GTTGAAGGGTTGTGATCATG+TGG 0.682135 7.1:-11037010 None:intergenic
TGGAATGCAAAGGTTGTGAG+AGG 0.687706 7.1:-11036936 None:intergenic
GTATGATGGAGGTCTATATG+TGG 0.691113 7.1:+11037588 MS.gene018718:CDS
CAATTAGTAGAGTATGATGG+AGG 0.693642 7.1:+11037577 MS.gene018718:CDS
CATGTGGTGATGGTTCAATG+CGG 0.842243 7.1:-11036994 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATATATTAATGATGAA+AGG + chr7.1:11037614-11037633 MS.gene018718:CDS 10.0%
!! AAAGGAAAATATCATGTTAA+AGG + chr7.1:11037363-11037382 MS.gene018718:CDS 20.0%
!! GATGATATAATACATTACAA+GGG + chr7.1:11037412-11037431 MS.gene018718:CDS 20.0%
!! TGATGATATAATACATTACA+AGG + chr7.1:11037411-11037430 MS.gene018718:CDS 20.0%
!!! AGTAATCTTGTTTTTTATTG+AGG + chr7.1:11037279-11037298 MS.gene018718:CDS 20.0%
!!! AGTAGAAAATAATTTTGATG+TGG - chr7.1:11036959-11036978 None:intergenic 20.0%
!!! GTAATCTTGTTTTTTATTGA+GGG + chr7.1:11037280-11037299 MS.gene018718:CDS 20.0%
! AAATCTTCTTGAAGATTTGT+GGG + chr7.1:11036820-11036839 MS.gene018718:CDS 25.0%
! ACTTTATGTTGTGGATAAAA+TGG + chr7.1:11037438-11037457 MS.gene018718:CDS 25.0%
! AGTCATCATGAAGATAAAAT+TGG + chr7.1:11037205-11037224 MS.gene018718:CDS 25.0%
! ATGGTTAGAAGTAAAAGATT+TGG + chr7.1:11037708-11037727 MS.gene018718:CDS 25.0%
! CTTTATGTTGTGGATAAAAT+GGG + chr7.1:11037439-11037458 MS.gene018718:CDS 25.0%
! TGGTTAGAAGTAAAAGATTT+GGG + chr7.1:11037709-11037728 MS.gene018718:CDS 25.0%
! TTATAAGCTTGATCAAGAAT+GGG + chr7.1:11037681-11037700 MS.gene018718:CDS 25.0%
! TTTATAAGCTTGATCAAGAA+TGG + chr7.1:11037680-11037699 MS.gene018718:CDS 25.0%
!! ATAAAATGGGAACAATTTTC+TGG + chr7.1:11037452-11037471 MS.gene018718:CDS 25.0%
!! ATTTTCCTTTTCTCCAATAT+TGG - chr7.1:11037353-11037372 None:intergenic 25.0%
!! CATCAAATCCAAAACTTTTT+CGG - chr7.1:11037146-11037165 None:intergenic 25.0%
!!! ATGATGAAAGGTATTACAAA+AGG + chr7.1:11037626-11037645 MS.gene018718:CDS 25.0%
!!! TGATGAAAGGTATTACAAAA+GGG + chr7.1:11037627-11037646 MS.gene018718:CDS 25.0%
AAGCAATTAGTAGAGTATGA+TGG + chr7.1:11037574-11037593 MS.gene018718:CDS 30.0%
ATCAAAGAGATAAAGTTTGG+AGG - chr7.1:11037086-11037105 None:intergenic 30.0%
ATCATGTTAAAGGATGATTG+TGG + chr7.1:11037373-11037392 MS.gene018718:CDS 30.0%
CAAATCTTCTTGAAGATTTG+TGG + chr7.1:11036819-11036838 MS.gene018718:CDS 30.0%
CTACTTCTATTATGAAAGCA+GGG + chr7.1:11037813-11037832 MS.gene018718:CDS 30.0%
GAGATCAAAGAGATAAAGTT+TGG - chr7.1:11037089-11037108 None:intergenic 30.0%
GATTAGAACAAGAAGTTGAA+GGG - chr7.1:11037027-11037046 None:intergenic 30.0%
TATAAGCTTGATCAAGAATG+GGG + chr7.1:11037682-11037701 MS.gene018718:CDS 30.0%
TCAACTTCTTGTTCTAATCA+AGG + chr7.1:11037028-11037047 MS.gene018718:CDS 30.0%
TCTACTTCTATTATGAAAGC+AGG + chr7.1:11037812-11037831 MS.gene018718:CDS 30.0%
TGAAAATTGAACCAACTTCA+AGG - chr7.1:11037491-11037510 None:intergenic 30.0%
TGATTAGAACAAGAAGTTGA+AGG - chr7.1:11037028-11037047 None:intergenic 30.0%
TGCTGAAAATAGGCATATAA+GGG + chr7.1:11037531-11037550 MS.gene018718:CDS 30.0%
TGTTTCCAATATTGGAGAAA+AGG + chr7.1:11037345-11037364 MS.gene018718:CDS 30.0%
! AGACACAAATTTTGCATTGT+TGG + chr7.1:11037756-11037775 MS.gene018718:CDS 30.0%
! GGAAAATACCGAAAAAGTTT+TGG + chr7.1:11037135-11037154 MS.gene018718:CDS 30.0%
!! AATTTTGATGTGGAATGCAA+AGG - chr7.1:11036949-11036968 None:intergenic 30.0%
!! GATGAAAGGTATTACAAAAG+GGG + chr7.1:11037628-11037647 MS.gene018718:CDS 30.0%
!!! AACTTTTTCGGTATTTTCCT+CGG - chr7.1:11037134-11037153 None:intergenic 30.0%
!!! AATCGTAGTGTTCAGTTTTT+TGG - chr7.1:11036855-11036874 None:intergenic 30.0%
ACTTCTTGTTCTAATCAAGG+TGG + chr7.1:11037031-11037050 MS.gene018718:CDS 35.0%
AGAAGTAAAAGATTTGGGTG+AGG + chr7.1:11037714-11037733 MS.gene018718:CDS 35.0%
CAATTAGTAGAGTATGATGG+AGG + chr7.1:11037577-11037596 MS.gene018718:CDS 35.0%
CTTCTTGTTCTAATCAAGGT+GGG + chr7.1:11037032-11037051 MS.gene018718:CDS 35.0%
GAGTTGACTGTTTCCAATAT+TGG + chr7.1:11037337-11037356 MS.gene018718:CDS 35.0%
GCCAAAATCTACAAACACTA+CGG - chr7.1:11036892-11036911 None:intergenic 35.0%
GTGCTGAAAATAGGCATATA+AGG + chr7.1:11037530-11037549 MS.gene018718:CDS 35.0%
TGTACCATTGTGCTGAAAAT+AGG + chr7.1:11037521-11037540 MS.gene018718:CDS 35.0%
TTGATCAAGAATGGGGTAAA+TGG + chr7.1:11037689-11037708 MS.gene018718:CDS 35.0%
! CTAAACCTGCTGACAAATTT+TGG + chr7.1:11037866-11037885 MS.gene018718:CDS 35.0%
! TATGCCTATTTTCAGCACAA+TGG - chr7.1:11037528-11037547 None:intergenic 35.0%
! TCCGTAGTGTTTGTAGATTT+TGG + chr7.1:11036888-11036907 MS.gene018718:CDS 35.0%
! TGGTTCAATGCGGTATATTT+TGG - chr7.1:11036987-11037006 None:intergenic 35.0%
!!! CAATGCGGTATATTTTGGTT+TGG - chr7.1:11036982-11037001 None:intergenic 35.0%
!!! GCACAATGGTACATGTTTTT+TGG - chr7.1:11037514-11037533 None:intergenic 35.0%
ATCACTGGATGGAACAAAGT+TGG - chr7.1:11037898-11037917 None:intergenic 40.0%
CAAAAGGGGTTACTTCTTGA+AGG + chr7.1:11037642-11037661 MS.gene018718:CDS 40.0%
CAAGGGACAACTTTATGTTG+TGG + chr7.1:11037429-11037448 MS.gene018718:CDS 40.0%
CAATGCTCTTTCCTTGAAGT+TGG + chr7.1:11037477-11037496 MS.gene018718:CDS 40.0%
CCAATCCAAAATTTGTCAGC+AGG - chr7.1:11037874-11037893 None:intergenic 40.0%
! CCTGCTGACAAATTTTGGAT+TGG + chr7.1:11037871-11037890 MS.gene018718:CDS 40.0%
! GTATGATGGAGGTCTATATG+TGG + chr7.1:11037588-11037607 MS.gene018718:CDS 40.0%
! TTTTATTGAGGGTCGTTGCA+TGG + chr7.1:11037291-11037310 MS.gene018718:CDS 40.0%
!! TGTTGGCTCGAGATTACTAT+GGG + chr7.1:11037773-11037792 MS.gene018718:CDS 40.0%
!! TTGTTGGCTCGAGATTACTA+TGG + chr7.1:11037772-11037791 MS.gene018718:CDS 40.0%
!!! CTTCTTGAAGGCTGTTTTTG+TGG + chr7.1:11037654-11037673 MS.gene018718:CDS 40.0%
GTTGAAGGGTTGTGATCATG+TGG - chr7.1:11037013-11037032 None:intergenic 45.0%
TGGAATGCAAAGGTTGTGAG+AGG - chr7.1:11036939-11036958 None:intergenic 45.0%
! GGGTGAGGTTTCGTTTGTTT+TGG + chr7.1:11037729-11037748 MS.gene018718:CDS 45.0%
! TTTCGGTATTTTCCTCGGCT+CGG - chr7.1:11037129-11037148 None:intergenic 45.0%
!! CATGTGGTGATGGTTCAATG+CGG - chr7.1:11036997-11037016 None:intergenic 45.0%
ATGCAAAGGTTGTGAGAGGC+AGG - chr7.1:11036935-11036954 None:intergenic 50.0%
GGGTTGTGATCATGTGGTGA+TGG - chr7.1:11037007-11037026 None:intergenic 50.0%
TAATACGAGAATCCGAGCCG+AGG + chr7.1:11037114-11037133 MS.gene018718:CDS 50.0%
CAAAGGTTGTGAGAGGCAGG+TGG - chr7.1:11036932-11036951 None:intergenic 55.0%
AGGTTGTGAGAGGCAGGTGG+AGG - chr7.1:11036929-11036948 None:intergenic 60.0%
TTGTGAGAGGCAGGTGGAGG+AGG - chr7.1:11036926-11036945 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 11036785 11037924 11036785 ID=MS.gene018718
chr7.1 mRNA 11036785 11037924 11036785 ID=MS.gene018718.t1;Parent=MS.gene018718
chr7.1 exon 11036785 11037924 11036785 ID=MS.gene018718.t1.exon1;Parent=MS.gene018718.t1
chr7.1 CDS 11036785 11037924 11036785 ID=cds.MS.gene018718.t1;Parent=MS.gene018718.t1
Gene Sequence

>MS.gene018718

ATGGATATGAATAAGATAGTGGAGTGTTCAAACTCAAATCTTCTTGAAGATTTGTGGGCAATTATTTCCAAAAAACTGAACACTACGATTGATGTTGTTCGATTCCGTAGTGTTTGTAGATTTTGGCGCTCTCTTCTTCCTCCTCCACCTGCCTCTCACAACCTTTGCATTCCACATCAAAATTATTTTCTACTCCAAACCAAAATATACCGCATTGAACCATCACCACATGATCACAACCCTTCAACTTCTTGTTCTAATCAAGGTGGGATCATTAAAGTTTTTCAAAATTCAAATTCCTCCAAACTTTATCTCTTTGATCTCTTCACTAATACGAGAATCCGAGCCGAGGAAAATACCGAAAAAGTTTTGGATTTGATGAACTTTCGAGTTGTTGAGTTGTCTAAAGTATATACTCGAAGTCATCATGAAGATAAAATTGGTTTTAAGTGTGCATCTCGTAATAATGTTTGCAATGTTTGCAATATTTGCAAAGTAATCTTGTTTTTTATTGAGGGTCGTTGCATGGTCTTTGCACTGCATAATGATAAAGAGTTGACTGTTTCCAATATTGGAGAAAAGGAAAATATCATGTTAAAGGATGATTGTGGAGAAAACAAATATTTTGATGATATAATACATTACAAGGGACAACTTTATGTTGTGGATAAAATGGGAACAATTTTCTGGATCAATGCTCTTTCCTTGAAGTTGGTTCAATTTTCACCAAAAAACATGTACCATTGTGCTGAAAATAGGCATATAAGGGTGAATTCTAACATAAACAAGAAGCAATTAGTAGAGTATGATGGAGGTCTATATGTGGTTGATTTATATATTAATGATGAAAGGTATTACAAAAGGGGTTACTTCTTGAAGGCTGTTTTTGTGGAAGTTTATAAGCTTGATCAAGAATGGGGTAAATGGTTAGAAGTAAAAGATTTGGGTGAGGTTTCGTTTGTTTTGGATAAAGACACAAATTTTGCATTGTTGGCTCGAGATTACTATGGGTGTGAAGAGAACTGCATCTACTTCTATTATGAAAGCAGGGCATCTTGTTTCAACTTGAAGAGTTTAGAGTCTAAACCTGCTGACAAATTTTGGATTGGTCCAACTTTGTTCCATCCAGTGATCAATTAA

Protein sequence

>MS.gene018718.t1

MDMNKIVECSNSNLLEDLWAIISKKLNTTIDVVRFRSVCRFWRSLLPPPPASHNLCIPHQNYFLLQTKIYRIEPSPHDHNPSTSCSNQGGIIKVFQNSNSSKLYLFDLFTNTRIRAEENTEKVLDLMNFRVVELSKVYTRSHHEDKIGFKCASRNNVCNVCNICKVILFFIEGRCMVFALHNDKELTVSNIGEKENIMLKDDCGENKYFDDIIHYKGQLYVVDKMGTIFWINALSLKLVQFSPKNMYHCAENRHIRVNSNINKKQLVEYDGGLYVVDLYINDERYYKRGYFLKAVFVEVYKLDQEWGKWLEVKDLGEVSFVLDKDTNFALLARDYYGCEENCIYFYYESRASCFNLKSLESKPADKFWIGPTLFHPVIN